CN1786180A - Method of improving byaluronic acid yield and molecular weight by fermentation process - Google Patents
Method of improving byaluronic acid yield and molecular weight by fermentation process Download PDFInfo
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- CN1786180A CN1786180A CN 200510122618 CN200510122618A CN1786180A CN 1786180 A CN1786180 A CN 1786180A CN 200510122618 CN200510122618 CN 200510122618 CN 200510122618 A CN200510122618 A CN 200510122618A CN 1786180 A CN1786180 A CN 1786180A
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- hyaluronic acid
- molecular weight
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- yeast powder
- fermentation
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention relates to a method of improving fermentation method producing hyaluronic acid yields and molecular weight. It belongs to hyaluronic acid fermentation technique field. Its steps is as follows: adopting streptococcus zooepidemicus H23 as start strain; producing hyaluronic acid by liquid fermentation; fermenting culture medium and using yeast powder as single nitrogen source; the yields and molecular weight of the hyaluronic acid is greatly increased at 180-220rpm rotate speed. It is applied to hyaluronic acid industrialization production. The invention can increase fermentation method producing hyaluronic acid yields and molecular weight, economize energy consumption, reduce production cost, and improve hyaluronic acid production quality.
Description
Technical field
The present invention is a kind of method that improves fermentative Production hyaluronic acid volume of production and molecular weight, relates to producing hyaluronic fermentation technical field.
Background technology
(Hyaluronic acid HA), has another name called hyaluronic acid or glass acid to hyaluronic acid, is a kind of macromole mucopolysaccharide, extensively is present in the reticular tissue of organism.The physiological action of HA is different in different tissues, as mainly show as water retention in skin; In knuckle synovia, be mainly lubrication; Mainly be to regulate permeability in vessel wall.In addition, HA is as polyanion electrolyte, on the molecule with a large amount of negative charges, can regulate the concentration of negative ions on every side, the activity that suppresses plurality of enzymes, be widely used in ophthalmology viscosurgery, treatment joint disease, soft tissue repair and as pharmaceutical carrier etc., particularly prevent and reduce after the surgical operation and obtained bigger progress in the tissue adhesion.
Adopt the Production by Microorganism Fermentation hyaluronic acid, the source of having widened HA has greatly promoted research and the application of HA.How traditional zymotic method production hyaluronic acid selects yeast powder and extractum carnis simultaneously for use is nitrogenous source, and adopts high mixing speed, and gained hyaluronic acid volume of production and molecular weight are all lower, have influenced the quality of final hyaluronic acid product.
Summary of the invention
The purpose of this invention is to provide a kind of method, simplification production technique that improves hyaluronic output of fermentative Production and molecular weight, to adapt to industrial needs.Use the present invention can improve the traditional zymotic method and produce hyaluronic zymotechnique, improve hyaluronic acid volume of production and molecular weight, improve the hyaluronic acid product quality.
Technical scheme of the present invention: adopt streptococcus zooepidemicus (Streptococcus zooepidemicus) H23 as starting strain, this bacterial classification is in " chemical industry journal " the 54th the 3rd phase of volume, P350-356, in March, 2003 is open, is provided by biotechnology institute of Southern Yangtze University; Produce hyaluronic acid through liquid fermenting, it is that single nitrogenous source, fermenting process employing mixing speed are 180～220rpm that fermention medium is selected 8.0～12.0g/L yeast powder for use, to improve hyaluronic output and molecular weight.
The used fermention medium of fermentative Production hyaluronic acid consists of: glucose 65.8g/L, yeast powder 8.0～12.0g/L, Na
2HPO
412H
2O6.2g/L, K
2SO
41.3g/L, MgSO
47H
2O2.0g/L, FeSO
47H
2O0.005g/L; Processing condition are: 37 ℃ of temperature, air flow 1.2vvm, best mixing speed are 200rpm.
Beneficial effect of the present invention: fermenting process adopts low mixing speed, hyaluronic output and molecular weight have been improved greatly, help industrial energy-saving and cost-reducing, the inventive method has been improved the traditional zymotic method and has been produced hyaluronic fermentation process, adopt separately yeast powder be nitrogenous source to reduce production costs, industrial production is of great importance.
Embodiment
Embodiment 1
Adopt streptococcus zooepidemicus (Streptococcus zooepidemicus) H23 as starting strain, produce hyaluronic acid through liquid fermenting, it is that single nitrogenous source, fermention medium consist of: glucose 65.8g/L that fermention medium is selected 8.0～12.0g/L yeast powder for use, yeast powder 8.0～12.0g/L, Na
2HPO
412H
2O6.2g/L, K
2SO
41.3g/L, MgSO
47H
2O2.0g/L, FeSO
47H
2O0.005g/L; Processing condition are: 37 ℃ of temperature, and air flow 1.2vvm, it is about 200rpm that fermenting process adopts mixing speed, and the gained hyaluronic acid volume of production reaches 6.0g/L, and molecular weight reaches 2.00 * 10
6Da.
Reference examples
Adopt streptococcus zooepidemicus (Streptococcus zooepidemicus) H23 as starting strain, produce hyaluronic acid through liquid fermenting, it is that other components of compound nitrogen source, fermention medium are with embodiment 1 that fermention medium is selected 8.0～12.0g/L yeast powder and 8.0～12.0g/L extractum carnis for use, it is about 650rpm that fermenting process adopts mixing speed, hyaluronic acid volume of production is about 3.8g/L, and molecular weight is about 1.21 * 10
6Da, two indexs all are lower than embodiment 1.
Claims (2)
1. method that improves fermentative Production hyaluronic acid volume of production and molecular weight, it is characterized in that adopting streptococcus zooepidemicus (Streptococcus zooepidemicus) H23 as starting strain, this bacterial classification is open at " chemical industry journal " the 54th volume the 3rd phase 350-356 page or leaf, produce hyaluronic acid through liquid fermenting, it is that single nitrogenous source, fermenting process employing mixing speed are 180～220rpm that fermention medium is selected 8.0～12.0g/L yeast powder for use, to improve hyaluronic output and molecular weight.
2. method according to claim 1 is characterized in that fermention medium consists of: glucose 65.8g/L, yeast powder 8.0～12.0g/L, Na
2HPO
412H
2O 6.2g/L, K
2SO
41.3g/L, MgSO
47H
2O2.0g/L, FeSO
47H
2O 0.005g/L; Processing condition are: mixing speed 200rpm, 37 ℃ of temperature, air flow 1.2vvm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510122618 CN1786180A (en) | 2005-11-23 | 2005-11-23 | Method of improving byaluronic acid yield and molecular weight by fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510122618 CN1786180A (en) | 2005-11-23 | 2005-11-23 | Method of improving byaluronic acid yield and molecular weight by fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1786180A true CN1786180A (en) | 2006-06-14 |
Family
ID=36783793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510122618 Pending CN1786180A (en) | 2005-11-23 | 2005-11-23 | Method of improving byaluronic acid yield and molecular weight by fermentation process |
Country Status (1)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101512006B (en) * | 2006-07-06 | 2012-04-11 | 利莱恩斯生命科学有限公司 | Efficient process for purification of high molecular weight hyaluronic acid |
| CN101935678B (en) * | 2009-06-30 | 2014-03-26 | 上海佰加壹医药有限公司 | Method for producing hyaluronic acid fermentation liquor |
| CN107198673A (en) * | 2017-05-26 | 2017-09-26 | 山东姣梨坊生物科技有限公司 | Marine organisms care compositions and its production method |
| CN108410927A (en) * | 2017-12-09 | 2018-08-17 | 新疆阜丰生物科技有限公司 | A kind of method of fermentation method production high molecular weight hyaluronic acid |
| CN109536550A (en) * | 2018-12-06 | 2019-03-29 | 上海景峰制药有限公司 | A kind of Sodium Hyaluronate and preparation method thereof |
-
2005
- 2005-11-23 CN CN 200510122618 patent/CN1786180A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101512006B (en) * | 2006-07-06 | 2012-04-11 | 利莱恩斯生命科学有限公司 | Efficient process for purification of high molecular weight hyaluronic acid |
| CN101935678B (en) * | 2009-06-30 | 2014-03-26 | 上海佰加壹医药有限公司 | Method for producing hyaluronic acid fermentation liquor |
| CN107198673A (en) * | 2017-05-26 | 2017-09-26 | 山东姣梨坊生物科技有限公司 | Marine organisms care compositions and its production method |
| CN108410927A (en) * | 2017-12-09 | 2018-08-17 | 新疆阜丰生物科技有限公司 | A kind of method of fermentation method production high molecular weight hyaluronic acid |
| CN109536550A (en) * | 2018-12-06 | 2019-03-29 | 上海景峰制药有限公司 | A kind of Sodium Hyaluronate and preparation method thereof |
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