CN1782092A - System for controlling pathogenic microorganism and stimulant and monitoring antiseptic use - Google Patents

Abstract

The present invention discloses products capable of killing harmful pathogenic microbes and protecting skin against damage of harmful irritant matter. The present invention provides antimicrobial toilet soap, antibacterial detergent composition, anti-bacterial skin care detergent liquid composition for local use on skin and glove inside capable of keeping moisture skin and shielding pollutant and irritant and one kind of verifying and monitoring kit. The present invention also provides method for detecting whether the employees are protected and are following the usage of the composition. The said products are applied jointly to form one system capable of monitoring pathogenic microbes and irritants. The present invention also provides great amount of other compositions, including scabies lotion, opacifier, spermicide, etc.

Description

The system that control pathogenic micro-organism and stimulator and monitoring antibacterials are used

Invention field

The present invention and U.S. Patent No. 5,676,994,5,882,713 and 6,238, the prepared a large amount of specificity group compounds of 677 disclosed methods are relevant, draw at this and to be reference, relate to the pathogen that is used for controlling in nursing and the cooking environments and the system of stimulator, and monitoring and verify test kit and method.

Background of invention

Skin is the largest organ of human body.Surprisingly skin almost can be contacted health with it anything separate.Skin also is that we resist outside contact and the effective first road barrier of coarse thing injury in the environment of our institute's live and work of protection, it has been generally acknowledged that making skin deliquescing and moistening best method is to use washing lotion and/or emulsifiable paste every day.The objectionable impurities that is touched in the skin of avoiding us and the daily life contacts no less important, and these materials comprise pollutent, toxin, bacterium and the pathogenic organisms in common homebrew, body fluid, perfumed soap, washing agent, acid, strong chemical products, the water.

Only the U.S. just has above 76,000,000 food poisoning case, wherein 300,000 hospitalizings every year.Have every year people more than 5,000 to die from the disease that food causes, wherein surpass 70% case and come from the catering service operation, nearly 40% case is all by seldom washing one's hands and crossed contamination causes.In the pathogenic micro-organism of being found in health care and catering service and stimulator, most important clinically family is exactly staphylococcus (Staphylococcus), and staphylococcus is divided into two main monoids: streptococcus aureus and non-streptococcus aureus.Streptococcus aureus is the virulence factor of soft tissue infection and toxin shock syndromes.Streptococcus aureus and other staphylococcic differences are: the zymoplasm test of streptococcus aureus is positive, and every other staphylococcus is then negative.Staphylococcic pathogenic effect is mainly relevant with the toxin that it produces.Most of toxin are to produce in the stationary phase of growth curve of bacteria.The toxin that streptococcus aureus produces can cause food poisoning rapidly, and this food poisoning causes spasm, and serious also can causing vomitted.Infection can track also not by the meat of the good pollution of culinary art.These microorganisms also secrete leueocidin.Leueocidin is a kind ofly can kill white corpuscle and cause the toxin of purulence and acne.Especially, streptococcus aureus (S.aureus) is found to be pneumonia, meningitis, furuncle, sacroiliitis, the virulence factor of osteomyelitis (chronic infection of bone).Most of streptococcus aureuses are penicillin tolerances, but vancomycin and NAFCILLIN can effectively be killed most of strains system.In non-streptococcus aureus, epidermidis strain (S.epidermidis) is the most important clinically.This microorganism is a kind of pathogenic former on the normal human subject skin of colonizing in.That be subject to this infectation of bacteria is following four class drug use persons: newborn infant, the elderly, the people of use conduit or other artificial apparatus.Be easy to after the infection treat with vancomycin and Rifampin.

Suis (Streptococcus) is formed by look like chain globular gram-positive microorganism at microscopically.The suis family member can be aerobic, anaerobic and microaerophilic.The feature of this class biology is grapelike profile and thick cell walls.Streptococcus pyogenes (Streptococcus pyogenes) produces a lot of toxin, comprises the erythrocytic hemolysin that breaks.Infection can be acute and cause the violent pain of epidermis of skin, infects also to become general.

The A group---first group comprises streptococcus pyogenes.The pathogenesis that this special opportunism pathogenic agent is about 90% pharyngitis.A kind of common form of pharyngitis is " septic sore throat (Strep throat) ", and its feature is throat swelling inflammation, grows the fritter that is full of pus on the tonsilla, and worsens into pneumonia.Also can develop into the fever that rheumatosis causes under some situation.The other diseases relevant with streptococcus pyogenes also has tetter such as pustulosis, phlegmon and erysipelas.

The B group---this B group only comprises a kind of bacterium, i.e. agalasisa bacterium (S.Agalactiae).This bacterium is the virulence factor of cow mazoitis always since a lot of years.Recently, find that it is a cause of disease of the urogenical infection that spreads through sex intercourse of women.

D group---this D type suis is another important clinically bacterium, known it can cause multiple disease.Although a lot of bacteriums are harmless, pathogenic bacterial strain can cause the complication in human digestive road.D type suis is divided into two classes recently: faecalis (Enterococcus) and non-faecalis (non-Enterococcus).Faecalis comprises that streptococcus faecium (E.faecalis) and streptococcus faecium belong to (E.faecium), and the former is the cause of disease of urinary tract infection; The latter can tolerate a lot of common microbiotic.Septicemia, endocarditis and ecphyaditis are microbial by D type hammer equally.D type suis also is one of normal human excretory flora.

Intestinal bacteria (E.Coli) are the research tools of whole world microorganism and genetics experiments chamber, also are the modal bacteriums of clinical laboratory.Except being one of the virulence factor of human urinary tract infection, intestinal bacteria almost also with the disease-related at each position of human body.Pneumonia, meningitis and traveller's diarrhoea all are the diseases that the intestinal bacteria pathogenic strain may cause.As the part of human intestine's normal flora, intestinal bacteria have played the part of crucial effects in food digestion, and it does not have to make vitamin K in the swill of digestion from large intestine.But, thereby colibacillary pathogenic strain can produce the serious diarrhoea that the very strong toxin of virulence causes the people of institute's has age.In fact the infection that causes with the antibiotic therapy intestinal bacteria may allow patient be in the lethal serious shock of possibility.This is because more bacteriotoxin is released when cell is killed.

A kind of aerobic, shaft-like, free-moving Gram-negative bacteria of energy, Rhodopseudomonas can produce intracellular toxin.It has the antibiotic resistance of height, can produce the blue-greenish colour crude granule.This pathogen is liked moist environment, and it has rich in protein.It is the opportunist of a height, but infection of burn person, diabetic subject; Most wound be can attack, serious respiratory tract disease and wound infection caused; Also can cause the hot tub dermatitis.

Hepatitis virus A, B, C type all can cause acute and chronic infection, and a distinguished feature is secular viremia, lasts up to the several months in acute infection, sustainablely in chronic infection reach several years or even throughout one's life.Hepatitis is by people's body fluid and contact transmission.

Skin pain, disease, the modal dermopathic cause of disease such as injured are to be exposed to for a long time in the environment of high temperature, radiation, chemical substance (as washing agent of solvent, alkali, acid, the usefulness of washing one's hands etc.), biological substance (body fluid, virus, bacterium, mould) and plant material (as the oil of Caulis Hederae Sinensis, Oak Tree, lacquer tree).It is wise avoiding as far as possible contacting with such stimulator.If have to around the known material that can cause skin problem or use these materials; their company should help to discern those Hazardous substancess so; advise safe work habit to them, and provide personal safety equipment to keep fit and be subjected to excellent protection with the skin that assists in ensuring that them.

Therefore, a kind of product of needs and system are controlled at pathogen and the stimulator in nursing and the cooking environments.

Summary of the invention

Therefore, the present invention relates to completely new product, it not only can kill harmful microorganism, can also protect skin to avoid the injury of objectionable impurities and stimulator.

The invention provides antimicrobial perfumed soap and the antibacterium surface cleaner composition of washing one's hands, the skin care washing lotion composition that uses on the skin and antibacterial part of using inside and outside the gloves also is provided, and this washing lotion can make skin keep moistening and shield unwanted pollutent and stimulator.The present invention also provides the detection employee whether to be subjected to protection, whether observes checking and the monitoring reagent box and the method for working method.This product is a system by combined utilization, is used for monitoring the pathogen and the stimulator of microorganism property.

Composition of the present invention pathogen of combating microorganisms character and continue 4-6 hour immediately after use.Even composition of the present invention in addition still can provide protection after washing 2-3 time with gentle perfumed soap and/or spirit solvent.

System of the present invention is made up of perfumed soap of the present invention, Liquid soap and surface cleaner, contains multiple antimicrobial " mixing ", can and kill pathogen with the diverse ways attack, makes pathogen unlikely to these products generation tolerances.

The present invention further provides many other compositions, comprised anti-scabies washing lotion, opalizer, spermicide, lipstick, antioxidant, anti-ageing/anti wrinkling agent and insect repellant.

Brief description of drawings

Fig. 1 has described by U.S. Patent No. 5,676, and 994,5,882,713 and 6,238,677 disclosed methods prepare method for compositions of the present invention.Wherein high temperature and violent turbulent flow can cause in the Wen chamber: the fully dissolving of a starch in water; B starch and oil thoroughly mix; The reduction of c shearing force inductive starch molecule amount;

Fig. 2 is a sem photograph, and it has shown prevailingly by United States Patent (USP) NO.5,676,994,5,882,713 and 6,238, and the product that 677 disclosed methods produce.It is that the scanning electron microscope of the starch granules separated the solution (Fantesk) of the dilution of making from W-Gum and soybean oil is shone.

Fig. 3 is a synoptic diagram, shows the different methods of making variant production composition of the present invention;

Fig. 4 is a column diagram, shows that the respondent is to whether oily evaluation of product of the present invention;

Fig. 5 is a column diagram, shows the evaluation of respondent to the quality of product of the present invention;

Fig. 6 is a column diagram, shows the evaluation of respondent to the viscosity of product of the present invention;

Fig. 7 is a synoptic diagram, and the aquation that has shown this product and other products is to the percentile evaluation of fat;

Fig. 8 is a linear graph, has shown the stamina after the use of product of the present invention;

Fig. 9 is a column diagram, shows when Viola crystallina is applied on the rough skin (contrast), has 100% painted; Quantity painted when being coated onto on the skin with other several commodity just drops to about 40-50%;

Figure 10 is a column diagram, shows when Viola crystallina is applied on the rough skin (contrast), has 100% painted; But when being coated onto on the skin with a kind of product of the present invention, nearly 20% is caught color;

Figure 11 is a column diagram, has shown the tolerance of product of the present invention to washing;

Figure 12 is a column diagram, has shown the tolerance that product of the present invention is washed alcohol;

Figure 13 is a column diagram, is presented at the lethality with this product residue after perfumed soap and the washing hand;

Figure 14 is a column diagram, is presented at the residual lethality behind perfumed soap and another product of washing;

Figure 15 is a linear graph, has shown after with product of the present invention the per-cent of skin wet;

Figure 16 has shown that two kinds of products of the present invention used the level of interpenetration of the corticoid on the corpse skin back 24 hours with comparing by a kind of product of former method manufacturing; After during 24 hours clinical important, hydrocortisone (HC) is to the bulking property evaluation of the level of interpenetration of human body skin: the product (CORTISONE 10 of PFIZER company) of skin care prescription (64E and 64B) and market sales volume first is compared;

Figure 17 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of streptococcus aureus (Staphylococcus Aureus).Experimentize chamber test is selected 1,000,000 bacterium-streptococcus aureuses and exposes to reach 15 minutes.Its kill rate is to calculate by the number that bacterium after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 18 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of formic acid tolerance streptococcus aureus.Experimentize chamber test is selected the streptococcus aureus of 1,000,000 bacteriums-formic acid tolerance and exposes to reach 15 minutes.Its kill rate is to calculate by the number that bacterium after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 19 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of Rhodopseudomonas pseudomonas bacteria (Pseudomonas Aeruginosa).Experimentize chamber test is selected 1,000,000 bacteriums-Rhodopseudomonas pseudomonas bacteria and exposes to reach 15 minutes.Its kill rate is to calculate by the number that bacterium after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 20 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of intestinal bacteria.Experimentize chamber test is selected 1,000,000 bacterium-intestinal bacteria and exposes to reach 15 minutes.Its kill rate is to calculate by the number that bacterium after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 21 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of streptococcus pyogenes.Experimentize chamber test selects 1,000,000 bacteriums-hinder streptococcus pyogenes and expose to reach 15 minutes.Its kill rate is to calculate by the number that bacterium after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 22 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of HIV.Experimentize chamber test is selected 1,000,000 bacterium-HIV1 and exposes to reach 15 minutes.Its kill rate is to calculate by the number that bacterium after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 23 is a column diagram, has shown that product of the present invention kills and wounds B, the time dependent per-cent that kills of hepatitis C virus.The test of extrapolating, the HIV1 that original position is selected extrapolates and exposes and reaches 15 minutes.Its kill rate is to calculate by the situation that virus after handling regrows, and the kill rate of handling after 15 minutes is 100%;

Figure 24 is a column diagram, has shown that product of the present invention kills and wounds the time dependent per-cent that kills of other pathogen.The pathogen of being tested comprises: bleb SV1/2, chlamydozoan, RSV, cytomegalovirus, adenovirus, RESP, SYNC virus, enterovirus, rhinovirus, polioencephalitis, rubella, measles, parotitis, rotavirus;

Figure 25 shown 4 volunteers received 24 hours airtight Finn cell (including aseptic solution or 1%-SLS, 2%-SLS and 4%-SLS) handle the clinical stimulation score chart of back skin site;

Figure 26 has shown with two kinds of different starch-oil mixt prescription DC-64A and DC-64E pre-treatment human skin and after 15 minutes, measured the efficient that this product prevents ICD in 24 hours with SLS closed processes people's skin.The frequency that the result does the clinical stimulation of X-coordinate, the appearance of given score with clinical stimulation mark is the ordinate zou mapping;

Figure 27 is a column diagram, has shown that prevention stimulates the inductive skin hydration, and specific practice is pretreated skin with 1%SLS or 2%SLS and water or separately with 1%SLS or 2% closed processes people's skin 24 hours.The ratio of the reaction of various processing and the reaction of water treatment is made X-coordinate, and standardized 1% hydration is an ordinate zou;

Figure 28 is a column diagram, shown undressed, handle with alcohol, with the bacterial growth number on the instrument in general hospital's environment of the surface cleaner compositions-treated of spraying of the present invention.The ZERO-UU=0-time is not cleaned and is untreated; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value is selected from 36m ²The selected regional numerical value in surface-at random all through overcorrection;

Figure 29 is a column diagram, has shown that the medical worker of general hospital is not washing one's hands, washing one's hands, passes through on hand when washing one's hands with the surface cleaner composition of spraying of the present invention gauged bacterial growth number with alcohol.The ZERO-UU=0-time is not cleaned and is untreated; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value be selected from one on hand-selected at random regional numerical value is all through overcorrection;

Figure 30 is a column diagram, the medical worker who has shown the general hospital do not wash one's hands, with alcohol wash one's hands, when washing one's hands with the surface cleaner composition of spraying of the present invention on hand without the bacterial growth number of overcorrection.The ZERO-UU=0-time is cleaning and processing not; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value be selected from one on hand-observed selected at random regional numerical value STD@1: 100 without gauged DIL;

Figure 31 is a column diagram, shown undressed, handle with alcohol, with the lip-deep bacterial growth number of surgery device of the surface cleaner compositions-treated of spraying of the present invention; The ZERO-UU=0-time is cleaning and processing not; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value is selected from 36m ²The selected regional numerical value in surface-at random all through overcorrection;

Figure 32 is a column diagram, shown undressed, handle with alcohol, with the gauged bacterial growth number of the ground process in general hospital of the surface cleaner compositions-treated of spraying of the present invention.The ZERO-UU=0-time is cleaning and processing not; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value is selected from 64m ²The selected ceramic tile numerical value in surface-at random all through overcorrection;

Figure 33 is a column diagram, shown undressed, handle with alcohol, with the gauged bacterial growth number of the lip-deep process of surgery device of the surface cleaner compositions-treated of spraying of the present invention; The ZERO-UU=0-time is cleaning and processing not; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value is selected from 64m ²The selected ceramic tile numerical value in surface-at random all through overcorrection;

Figure 34 is a column diagram, shown undressed, handle with alcohol, with the ground bacterial growth number in general hospital of the surface cleaner compositions-treated of spraying of the present invention without overcorrection.The ZERO-UU=0-time is cleaning and processing not; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value is selected from 64m ²-selected at random ceramic tile numerical value;

Figure 35 is a column diagram, shown undressed, handle with alcohol, with the ground bacterial growth number of Surgical Operating Room of the surface cleaner compositions-treated of spraying of the present invention without overcorrection.The ZERO-UU=0-time is cleaning and processing not; The ZERO-ALC=0-time is cleaned with alcohol; DC=handles with skin care product SC.Numerical value is selected from 64m ²The selected ceramic tile numerical value in surface-at random.

Embodiment

The present invention has prepared the composition of two kinds of different topical application, and called after composition DC-64A and DC-64E.The former is a kind of washing lotion, and it is to be prepared from the method for 10 parts of Vaseline gels of 100 parts of waxy W-Gums mixing by spraying-culinary art; This lotion in back is to be prepared into the method for 50 parts of Vaseline gels of 100 parts of waxy W-Gums mixing by spraying-culinary art.Like this spraying of Xing Chenging-culinary art emulsion with the gas blower drying, be milled into smalls (＜0.5 millimeter) and be stored in (＜5%) under the low humidity condition.Pressed powder is with the dissolving of aseptic deionized water melt into 20% (w/v), and the flowable liquids of generation can directly use.Product of the present invention is presented among Fig. 2.The every other product that this institute uses all from Sigma chemical company buy (St.Louis, MO).Fig. 1 show that the method be used for making product of the present invention, Fig. 3 show to have given birth to and produce circulation.

Safety date sheet (the Material Safety Data Sheet of following a part of material, MSDS) shown is the composition of antimicrobial fluid of the present invention, comprising every kind of composition preferably use mass percent, be the scope of every kind of spendable mass percent of composition in the parenthesis.

Pure water-87.75% (80.0-99.5%)

Waxy starches or polysaccharide (natural or synthetic)-7% (0.5-15.0%)

Vaseline or other mineral oil-3% (0.125-12.0%)

Other plant/vegetable oil or extract-0.25% (0.01-8.0%)

Pectin or other viscosity stabilizers-0.5% (0.01-3.0%)

Xanthine or other viscosity intensifiers-0.5% (0.01-3.0%)

D-alpha-tocopherol or other antioxidants or stablizer-0.15% (0.01-5.0%)

Phenylformic acid-0.1%-can select (0.01-1.0%)

Boric acid-0.1%-can select (0.01-1.0%)

Magnesium chloride-0.05% (0.01-1.0%)

Benzoin methyl acid phenolic ester-0.1% (0.01-1.0%)

Propyl group M-nitro benzoic acid phenolic ester-0.1% (0.01-1.0%)

Citricidal-0.1％(0.01-3.0％)

Benzalkonium chloride or other are demarcated the quartemary ammonium compound-0.35% (0.01-2.0%) in the concentration

3N-butyl PO ₄-0.125% (0.01-1.0%)

Can contain-benzoyl peroxide compound-1.0%@q.s. (0.05-3.0%)

Male, women healthy volunteer has 26: in the research of A item 4 people (the pilot evaluation of dosage range) are arranged, 22 people (the main evaluation) are arranged in the research of B item, comprise standard: the volunteer fills in agreement, volunteer's minimal ages is 18 years old, there are men and women among the volunteer, the volunteer has the physique condition of drug regulation, supposes that they did not accept any one medicine of listing in the exclusion standard; Exclusion standard: the women does not necessarily have pregnancy, if pregnancy must be practised contraception.In order to prevent dermopathicly to send out, following a few class volunteers are left out again: be in inflammatory acne active period, that can not be controlled by pharmacological agent or infect the patient; The back has other to be in the skin patient of nonmobile phase; Suffered from eczema, psoriasic patient recently.Adhesive tape, washing agent, human immunodeficiency virus, hepatitis allergies and in 6 months exclusion condition irritated to immunosuppressant therapy (chemotherapy that comprises cancer) and that other doctors see fit all be to get rid of the factor.In addition, the pharmacological agent that is excluded comprises that prednisone, diuretic(s), steroid are used in the back in one month, or other eliminating factors of doctor's approval.At last, lung allergy sufferers or the extremely sensitive person of washing agent is left out.

Carry out skin patch test with sodium lauryl sulphate

Figure 26 and 27 has shown some results of this test.Anticipate or do not anticipate with emulsion of the present invention, use sodium lauryl sulphate (SLS, known stimulator) to carry out skin patch test again.Detect by an unaided eye and skin electric capacity instrument (model C M825 Courageand Kahazaka, Cologne, Germany) stimulation is marked.Variable is the concentration of SLS and whether carries out pre-treatment with emulsion.The mode standard of using ICD (stimulating the contact dermatitis) in these researchs is the closed skin patch test that washing agent sodium lauryl sulphate (SLS) stimulates.The closed skin patch test of SLS is proved to be a good method.SLS skin patch test is used for estimating the effectiveness of skin care fat always, with 1,2 or the aqueous solution of 4%SLS molten long-pending be that use reaches 24 hours in the Finn cell of " especially big " of 18mm for the 0.3ml diameter.With the water dissolution of high purity (＞99%) SLS with the USP level, the aqueous solution that is made into is placed in the sizeable filter disc, inject Finn cell (Epitest, Hermal pharmaceutical test chamber, Oak Hill, New York) then.(Epitest) is fixed to this cell on the skin of back with adhesive tape.Experimenter's skin of test site of can not getting wet in 24 hours.Move the test cell after 24 hours, with the naked eye to stimulating scoring.Tested preceding 15 minutes at SLS Finn cell, on skin of back, coat emulsion.The target zones at back is defined as the circular cell of 2.0 centimetres of diameters.Emulsion measures out with 1.0 milliliters transfer pipet.Coat 0.3ml emulsion with glove finger 2.0 centimetres circular cell.

With the naked eye grade scoring is carried out in stimulation

Between changing, erythema oedema and epidermis be divided into four grades in proportion: 0, not change, and to 4+, erythema oedema, epidermis erosion.The result of macroscopic score shows that the protection effect of skin care breast in SLS skin patch experimental study has significant statistics difference.

The humidity evaluation in skin patch experiment site

The electric capacity instrument(model C M825 Courage and Kahazaka, Cologne, Germany) all is used for testing after skin is handled by the airtight skin patch experiment of 2%SLS the capacitance variations with respect to the skin of the hydrated state of skin all the time.The experimenter stops to test in back 5 minutes, the electric capacity in whole 12 sites that every experimenter of electricity consumption looks mensuration accepts to handle.Each site is measured 10 times, calculates mean value and deviation by the software program that the supplier provides.Skin Hydration value all is to be 5 by instrument with background reading (dried paper handkerchief), and wetted paper handkerchief reading is 110 to proofread and correct.Carry out a large amount of checking research and draw numerical value reliable and stable on the statistics to guarantee suitable test condition.These comprise the temperature (24-25 ℃) when allowing the experimenter adapt to experiment in 30 minutes in advance, control relative humidity (50-60%), and the experimenter provides repeatably distinctive datum readings under such condition.

This research is divided into preliminary stage, A part, and later stage, B part.A partly is a preliminary study, determines the optimized scope of the SLS that skin irritation inflammation institute uses.B partly is the main body evaluation, and test emulsion protection skin is avoided the ability that SLS stimulates.With solution numbering, the technician does not know the concentration of SLS, but the unlikely technician of allowing can't see the skin site that is subject to processing, so the people of evaluating skin patch experiment do not know processing mode, does not know the application model of test soln.Same result allows three people's readings, the comparative evaluation result.

The A part, the experimenter is received in the following processing that their back carries out.Be to coat 0.3ml emulsion on the 2cm skin at diameter earlier, after 15 minutes, the Finn cell of the SLS that is filled with 0.3ml tied up on the skin site.Following treatment group is pressed matrix random assignment at random: 1 group and 2 groups, and SLS-1.0%; 3 groups and 4 groups, SLS-2.0%; 5 groups and 6 groups, SLS-4%; 7 groups and 8 groups, the solvent of SLS (water).The experimenter can not allow test site contact water in 24 hours.Take away the Finn cell after 24 hours, with the naked eye classification (referring to following table 1) is carried out in stimulation.All test site all are filmed and are equipped with card.Above-mentioned evaluation is used for determining producing irritant reaction intensity when washing lotion pre-treatment of no use be 2 or 3 SLS concentration.

Table 1. carries out the fractionated standard with vision to irritant reaction

0=does not change, and does not have erythema

1+=slight erythema occurs in most treatment site

2+=tangible erythema occurs in all treatment site, and has slight oedema

Oedema appears in 3+=, and skin is blistered

The 4+=epidermis is corroded

The solution of two kinds of concentration having determined above B part: B has partly used.Have 22 experimenters.In order to control experimenter's self variation.Each experimenter has chosen 12 sites altogether and has carried out repeated test.All sites all is selected on the skin of back between waist and the clavicle.Following treatment group is pressed matrix random assignment at random: 1) handle with 1%SLS; 2) use the DC-64E pre-treatment, handle with 1%SLS again; 3) handle with 2%SLS; 4): use the DC-64E pre-treatment, handle with 2%SLS again; 5) use the DC-64A pre-treatment, handle with 1%SLS again; 6) use the DC-64A pre-treatment, use the solvent (water) of SLS to handle again.With swab the emulsion of 0.3ml is coated onto in the border circular areas that diameter is 2cm earlier.After 15 minutes, the SLS solution of 0.3ml is injected into 18mm Finn cell as follows.The technician uses all treatment group of Finn cell matrix random assignment.The experimenter can not allow test site contact water in 24 hours.Take away the Finn cell after 24 hours, classification is carried out in stimulation with vision scoring and skin electric capacity instrument (Corneometer).The investigator who skin site is marked with vision and skin electric capacity instrument knows nothing treatment site.All test site all are filmed and are equipped with card.

Statistical study

In the skin patch experiment of doing with SLS, vision points-scoring system and the test of electric capacity instrument all provide the sensitivity that has remarkable meaning on the statistics.In the past barrier cream frost and SLS skin patch experiment have only just provided the result who has remarkable meaning on the statistics with 10 experimenters' vision points-scoring system.Research B has used 22 experimenters, and the have remarkable statistical significance result (stimulating difference according to 20%) of (P＜0.05, α＞80%) is provided equally.The vision scoring is not a variable data, analyzes by chi square test is carried out in the pairing of treatment site and non-treatment site.Capacitance data is with analysis of variance (ANOVA), treatment site and non-treatment site matched T-examine and determine and analyze.

Adverse events

During the whole research, main investigator monitors each experimenter, because the intolerance on the program has appearred in some experimenter, irrational clinically symptom has appearred in other experimenters.Should be noted that the adverse events in this research is as expected owing to the reaction to SLS.All incidents of getting rid of in this research process all are documented in the suitable case report volume, and are filmed card fully, and continue to follow the tracks of up to satisfied solution is arranged.If expect outer or when the investigator has to experimental arrangement that very the reaction of trouble takes place, handle no matter which kind of has been accepted, the investigator provides suitable therapeutic treatment.Have in three cases of untoward reaction in the application to material, we advise that the experimenter stops experiment or the experimenter has stopped experiment voluntarily.That is done during studying is used for proving that testing or estimate any test or evaluation that the significance difference is arranged corresponding early stage all must defer to certain medical science at interval, eliminates up to abnormality.There are not serious or unexpected untoward reaction generation.

SLS concentration range research (A part) proves that 1% and 2% SLS just is enough to induce erythema and the corrosion that clinical evaluation is an intermediate range.And the reaction of 4% SLS inductive is too strong, can not carry out the research (Figure 25) of back, in part B, has selected two main evaluations of SLS concentration work that irritant reaction is 2-3.As expection, the intensity of irritant reaction and the concentration of SLS linear (correlation coefficient r=0.93, p＜0.05).

The main body evaluation result summary of the clinical stimulation evaluation portion of all experimenters sees Table 2,22 initial experimenters in this research, only has 20 to finish agreement.Post 1 and 2 is represented two independently skin site (left side at back and the right), and they handle with 1%SLS.Average clinical stimulation mark is 2.6 ± 0.8 and 2.5 ± 1.1.(post 5 and 6) 0.3 ± 0.6 and 0.2 ± 0.4 of this and two independently water treatment skin site formed striking contrast.Obtained similar result with the 2%SLS processing, clinical stimulation mark is 3.2 ± 1.0 and 2.9 ± 1.4 (posts 3 and 4).These data show: with respect to 1%SLS and water treatment, handle with 2%SLS, the seriousness of skin reaction strengthens.Table 2 has been listed and has been accepted preceding 15 minutes of 1%SLS (post 7 and 8) or 2%SLS (post 9 and 10) closed processes, with the validity of emulsion DC-64A pre-treatment skin site.The average score of DC-64A-1%SLS treatment group is 1.3 ± 0.1 and 1.3 ± 0.8.These results have proved the skin effect that emulsion DC-64A opposing 1%SLS stimulates.Equally, before accepting the 1%SLS processing,, drawn the protection effect (average clinical stimulation mark 1.5 ± 0.9 and 1.5 ± 0.9) of highly significant with emulsion DC-64E pre-treatment skin site.When handling, also observed the protection effect (average clinical scores 2.0 ± 1.3 and 1.8 ± 1.3) of DC-64E with 2%SLS.Last column of table 2 has been summed up the average clinical scores after each treatment group is rounded up to nearest integer.Average score reaction be the situation (No. 12 experimenters) of having removed after the value.

The allergy (clinical stimulation mark) that table 2 causes with DC-64A and the airtight skin of DC-64E washing lotion prevention tensio-active agent

M/I	SLS- 1％	SLS- 1％	SLS- 2％	SLS- 2％	Wat er	Wat er	64A +1％ sls	64A + 1％sl s	64E+ 1％sl s	64E+ 1％sl s	64E+ 2％sl s	64E+ 2％sl s
													1/ afh	2	3	4	4	0	0	2	3	0	3	3	2
2/ hjh	3	3	4	4	0	0	0	0	1	1	1	3
													3/ sds	2	1	4	4	1	0	2	1	1	1	1	2
4/trf	3	4	4	4	0	0	2	1	2	2	3	0
													6/aa m	2	2	0	0	2	1	0	1	3	1	3	3
7/n- r	3	3	3	1	0	0	1	2	2	1	2	2
													8/ paw	3	3	3	1	0	1	0	1	1	0	1	0
9/jes	2	3	3	4	0	0	1	0	1	1	4	3

11/ dal	3	3	3	3	0	0	3	2	2	2	3	3
													12/kt p	0	0	2	1	0	0	2	1	1	1	1	1
13/s ms	2	3	4	4	0	0	1	1	2	2	3	4
													14/ jah	2	2	2	3	0	1	0	1	0	1	1	0
15/ dkl	3	4	2	3	0	0	1	1	1	1	3	0
													16l/s g	2	1	4	1	2	1	1	1	3	3	1	2
17/ ljl	3	3	3	2	0	0	3	2	1	3	1	2
													18/ d-s	3	2	4	4	0	0	3	2	3	2	3	3
19/k kg	4	2	4	4	0	0	1	2	1	1	3	0
													20/tt m	3	1	3	3	0	0	1	2	1	0	0	2
21/ lea	3	3	4	4	0	0	1	0	2	2	1	1
													22/ tal	3	4	4	4	0	0	1	2	2	2	4	3

X ±sd N＝2 0	2.4± 1.0	2.5 ±1.1	3.2± 1.0	2.9± 1.4	0.3± 0.6	0.2± 0.4	1.3± 1.0	1.3± 0.8	1.5± 0.9	1.5± 0.9	2.0± 1.3	1.8± 1.3
													Scor e	2	3	3	3	0	0	1	1	2	2	2	2

M, experimenter's numbering; I, initial letter; 64A+1%sls, 64E-1%sls, 64E-2%sls=carry out the Derm-Care that washing lotion is handled before SLS handles ^TM

SLS handles caused skin hydration and strengthens

Following table 3 is summaries of all 20 experimenter's skin capacitive readings.According to former research and our internal contrast, we think that the aquation of skin strengthens to the skin capacitive reading that raises.The several letters in front of experimenter's numbering and name have been listed on hurdle 1 and hurdle 2 respectively.The skin electric capacity of every experimenter's sacral midline left and right sides measuring in 24 hours before airtight patch experiment the results are shown in B1 and B2 hurdle.Clearly experimenter's skin capacitive reading interindividual variation is between 46 to 76, and however, intraindividual difference has significant consistence (left side average=58 ± 8 less than 5% of average between the data on left side and right side; Right side average=58 ± 8).The average of the skin site of water treatment (hurdle 5 and hurdle 6) is 47 ± 10 and 46 ± 9.The value that is noted that two people foreclose (No. 6 and No. 13 experimenters).The average of the skin site of water treatment is less than radix, but and do not have difference (P＞0.06) on the statistical significance between the average of the skin site handled of 1%SLS, the latter's average is 104 ± 14 and 98 ± 19.5 experimenters do not add up interior, because their value is low especially.Under every kind of situation, the low especially reading of registration always be subjected to the corrosive skin site on the death of the face tissue that occurs relevant.The average of the skin site that 2%SLS handles is that 100 ± 20 and 94 ± 29.5 experimenter forecloses being in once more, because their reading and 1%SLS treatment group reading are in fact closely similar.In a word, 1% and the skin site handled of 2%SLS represented very high skin hydration level significantly.

The skin hydration (verification reading) that table 3DC-64A and DC-64E washing lotion prevention SLS cause

(Coineomiet icauiags)

		B	B	SL- 1％	SL- 1％	SL- 2％	SL- 2％	W	W	64 A- 1％	64 A- 1％	64 E- 1％	64 E- 1％	64 E- 2％	64E- 2％
																M	I	L	R	1	2	3	4	5	6	7	8	9	10	11	12
1	afh	54± 2	67± 3	114 ±8	116 ±11	121 ±10	122 ±10	62± 6	55± 6	52± 5	76± 5	50± 3	88± 4	67± 5	84± 10
																2	hjh	49± 3	58± 2	55± 3	55± 6	101 ±3	108 ±15	47± 3	57± 2	58± 3	53± 1	45± 3	43± 4	72± 5	53± 8
3	sds	61± 4	53± 3	25± 3	38± 5	114 ±7	96± 16	59± 5	46± 4	57± 5	38± 8	47± 3	24± 2	36± 6	41± 7
																4	trf	55± 2	59± 1	90± 14	109 ±9	115 ±6	121 ±0	49± 3	47± 5	54± 4	59± 3	55± 6	57± 6	48± 8	41± 4
6	aam	76± 4	74± 2	81± 4	63± 5	76± 5	59± 4	98± 6	65± 4	67± 7	72± 4	78± 6	97± 11	104 ±11	83± 5
																7	m-r	53± 2	61± 3	82± 11	99± 11	74± 10	45± 15	25± 1	33± 2	32± 3	38± 6	25± 1	30± 3	52± 7	59± 5
8	paw	55± 2	52± 1	95± 10	104 ±8	52± 4	33± 4	46± 4	42± 5	45± 3	30± 4	39± 9	55± 6	49± 7	45± 5
																9	jes	58± 2	52± 2	109 ±12	117 ±8	87± 8	66± 6	45± 6	46± 4	57± 10	30± 5	46± 4	49± 4	58± 6	76± 13
11	dal	58± 2	59± 4	120 ±1	121 ±2	84± 6	93± 8	42± 4	61± 5	90± 6	96± 9	103 ±12	75± 11	79± 7	65± 4
																13	sms	59± 3	64± 2	118 ±3	120 ±1	121 ±1	121 ±1	113 ±5	75± 5	118 ±5	121 ±6	112 ±6	113 ±11	90± 7	110± 10
14	jah	50± 1	47± 3	117 ±3	100 ±8	44± 4	101 ±6	47± 2	38± 13	25± 1	28± 2	36± 3	30± 2	29± 4	34± 11

15	dkl	76± 1	70± 2	104 ±9	102 ±6	67± 4	92± 11	52± 3	43± 5	30± 8	29± 3	42± 5	65± 24	107 ±18	66± 5
																16	lsg	62± 2	58± 2	33± 6	41± 7	121 ±2	30± 2	64± 6	50± 3	25± 1	30± 5	27± 2	52± 5	33± 4	38± 9
17	ljl	66± 10	67± 4	109 ±5	75± 5	47± 5	87± 6	36± 2	50± 6	71± 5	30± 2	39± 2	71± 7	32± 2	63± 8
																18	d-s	56± 2	56± 1	116 ±10	106 ±7	113 ±7	113 ±8	45± 2	43± 3	79± 5	79± 8	75± 9	81± 5	99± 14	119± 4
19	kkg	46± 3	43± 2	81± 5	70± 10	47± 8	98± 12	39± 6	28± 1	30± 3	25± 3	39± 7	37± 6	40± 4	36± 2
																20	ttm	55± 2	54± 2	116 ±3	43± 9	112 ±1	102 ±6	34± 3	34± 3	29± 4	46± 1	45± 4	63± 2	30± 5	59± 4
21	lea	58± 4	55± 1	38± 9	81± 12	68± 8	33± 6	46± 5	41± 1	47± 3	29± 5	39± 6	61± 4	30± 4	41± 6
																22	tal	60± 1	63± 3	67± 7	73± 6	109 ±15	119 ±0	54± 2	55± 1	33± 3	64± 7	46± 11	73± 4	74± 12	73± 4
23	ktp	53± 3	56± 2	104 ± 15	82± 8	100 ±4	76± 13	55± 6	52± 4	77± 5	47± 7	32± 2	71± 19	45± 8	35± 6

The M=numbering; The I=initial letter; The B=radix; SL=SDS; W=water; The 64A/64E washing lotion

Handle the mean level (ML) (48 ± 18 and 47 ± 21) that skin site has reduced the skin hydration of the sacral midline left and right sides significantly with emulsion DC-64A in advance.These average slaking velues do not comprise 2 experimenters (No. 6 and No. 13 experimenters') value.Skin site with the emulsion DC-64E pre-treatment left and right sides has also reduced the skin hydration level (45 ± 14 and 45 ± 14) that is caused by 1%SLS significantly.Two experimenters above-mentioned (No. 6 and No. 13 experimenters) are left out once more.At last, also reduced the stronger skin hydration level (54 ± 22 and 55 ± 17) that causes by 2%SLS significantly with the skin site of the prescription DC-64E pre-treatment left and right sides.

In a word, the result shows that use starch provided by the present invention-oil mixt emulsion has fabulous skin care characteristic.The process of exclusive oil-in-water dispersion has formed one deck polymer protective film on complete skin.This layer protective membrane stoped the skin (even under airtight condition) of the stimulator transdermal of water miscible picture SLS and so on.The protective effect with the dispersive droplet that flocks together is based on it and can keeps skin directly to contact the skin wet in territory, and this has obtained proof.The protective effect of saccharan is that it has formed one deck hydration layer, and this hydration layer absorbs moisture, prevents that the skin contact district from being permeated by water miscible stimulant.Do not have the protection of barrier cream, the skin site of handling with SLS has clinical stimulation mark of remarkable enhanced and skin hydration.Generally speaking, clinical response result and skin hydration result are starch-oil mixt emulsion provides strong evidence as a kind of protective effect of barrier cream.According to above result, our suggestion has the ability to prevent the untoward reaction that skin takes place when running into many present skin cares and Wound care products as the starch-oil mixt of emulsion of the present invention and so on.

A composition of the present invention is a kind ofly can not only kill harmful microorganism, and can protect skin to avoid the product of objectionable impurities and stimulator injury.Antimicrobial compound contains such composition: it can moistening skin forms simultaneously, and one deck be cannot see, undetectable macromolecule membrane, this layer film covers the skin appearance layer, in it is enclosed in the natural wettable layer of skin, outside undesired pollutent and stimulator be blocked in.When instruct using, this antimicrobial compound when not reusing sustainable 4 to 6 hours.Shown in Figure 11-13, even product of the present invention still had the protection effect after washing with gentle perfumed soap and spirit solvent.Once use, the dispersive droplet will pass exodermis (stratum corneum) thereby strengthen its fat protection to water moderate stimulation thing.Saccharan matrix adheres to the stratum corneum outside that grease covers and forms the protection barrier, so just formed a sealing the barrier of opposing nocuity surrounding material.

An antimicrobial compound of the present invention is a kind of brand-new starch-oily suspended mixture, and it is by spraying-heating and pressure process preparation, is made up of oil, starch, polysaccharide.Specific preparation of compositions has been become the skin of protecting to greatest extent, improve that can provide to safeguard and moistening suitable emulsion, by vegetables oil, mineral oil or Vaseline and high-molecular weight starch are mixed, disperse to finish this process to skin.Using the oil of specific blend and starch is that this antimicrobial compound feels very soft and the reason of texture is arranged very much.This composition has produced a kind of skin mildness and has kept system, this washing agent with most of oil-containing water mixtures is different, the most important thing is that these compositions can mix, and do not need to use the chemical stabilizer that exists in any irritating tensio-active agent or emulsifying agent (surface-active agents) or other the most of skin care products.Compare with the washing agent of most of oil-containing-aqueous emulsions, this composition is also produced the slick barrier of a kind of height.It is also important that it is the component insensitivity that antimicrobial component of the present invention is stablized the active activeconstituents of pH value competence exertion to the needs that add, this specific character makes that it can be compatible with the skin analeptic with VITAMIN, antioxidant.

A large amount of physiology and human test show that composition of the present invention has formed the high molecular protective film of one deck on skin.The protection effect can compare favourably with the product of many first-class famous skin care brands.The skin hydration test of reflection protection effect (minimizing of moisture of skin loss) is the result be summarised among Fig. 7.The performance of the present composition has exceeded the product of every other test.In order to obtain the protection effect, just other products of great majority must be coated with the last layer grease effectively, have so just caused residual one deck oily grease on the skin.Yet prescription of the present invention can not stay the oily grease on skin.This point can remaining greasy Sebumeter device to test proves (see figure 7) on the skin with measuring by the laboratory.

A kind of composition of the present invention is the skin moisturizing washing lotion, and it is formulated with the natural moisture preserving agent, and with respect to the skin moisture-keeping product on other markets, it has very outstanding moistening and hydration characteristics.This product also is based on the emulsion technology and utilizes a kind of special complete stabilize oil aqueous emulsion transparent and polysaccharide-Simethicone that do not have greasy feeling formulated, and it can preserve moisture and protect skin, has fabulous feel again.Fig. 4 and 6 has shown the marking that the respondent provides greasy, the quality and the viscosity aspect of product of the present invention.

Anti-bacterial lotion of the present invention is that specialized designs is come the enantiopathy pathogenic microorganism.Composition of the present invention has very wide antimicrobial spectrum, and communicable streptococcus aureus, Colibacter, suis, HIV, intestinal bacteria and Rhodopseudomonas pseudomonas bacteria are all had antibacterial effect.See Figure 17-24, shown with the kill rate of composition of the present invention to pathogen.

Product of the present invention occurs with multiple different form.Protection persistency when using with spray method is presented among Fig. 8.The viscose glue paste composite is seen Figure 15 to the effect of skin wet.Be the MSDS tabulation of the composition of cleanser/hand composition of the present invention below, it has shown the massfraction that each composition preferably uses, and is the scope of every kind of spendable mass percent of composition in the parenthesis.

Pure water-96.35% (85.0-99.5%)

N, two (2-OH-ethyl) laurylamides-1.0% (0.25-5.0%) of N-

The sodium sulfonate of C14-C16 paraffin-0.5% (0.05-3.0%)

Polyoxyethylene glycol (1-4) bay ether SO4 (sodium salt)-0.35% (0.03-3.5%)

Polyquaternium-7 (Polyquaternium-7)-0.30% (0.015-2.5%)

Chlohexidine-1% (0.25-4.0%)

Vegetable fatty acid (selectable)-0.25% (0.02-1.5%)

Antioxidant-0.2% (0.05-1.0%)

Phenol-0.3% (0.015-1.75%)

Be the MSDS tabulation of composition of the surface cleaner composition of spraying of the present invention below, it has shown the massfraction that each composition preferably uses, and is the scope of every kind of spendable mass percent of composition in the parenthesis.

Pure water-96.35% (85.0-99.5%)

5H ₂The O water glass

Nonoxynol-9 or congener-0.25% (0.02-2.5%)

Butyl glycol ether-2.0% (0.05-5.5%)

50% Barquat 50 solution-0.60 (0.05-3.5%)

Barlox 12-0.25％(0.01-1.5％)

May contain-benzoyl peroxide compound-1.0%@q.s. (0.05-3.0%)

Below composition can join in the top composition with stable and strengthen residual lethality surperficial pathogen, shown the massfraction that each composition preferably uses below, be the scope of every kind of spendable mass percent of composition in the parenthesis:

Waxy starches or other polysaccharide (natural or synthetic)-1% (0.05-2.5%)

Vaseline or other mineral oil-0.5% (0.125-2.0%)

Other plant/vegetable oil or extract-0.15% (0.01-2.0%)

Jelly or other viscosity stabilizers-0.05% (0.01-1.0%)

Xanthine or other viscosity intensifiers-0.05% (0.01-3.0%)

D-alpha-tocopherol or other antioxidants or stablizer-0.05% (0.01-1.0%)

Phenylformic acid-0.1%-selectable (0.01-1.0%)

Boric acid-0.1%-selectable (0.01-1.0%).

Figure 28-35 has shown respectively by column diagram: the bacterial growth number on the instrument in general hospital's environment, medical worker's bacterial growth number on hand through gauged hospital, medical worker's bacterial growth number on hand without the hospital of overcorrection, the lip-deep bacterial growth number of surgery device, through the ground bacterial growth number in gauged general hospital, through the lip-deep bacterial growth number of gauged surgery device, without the ground bacterial growth number in the general hospital of overcorrection, without the ground bacterial growth number of the Surgical Operating Room of overcorrection.Sordid, the undressed instrument of choosing, on hand, on the surface and the number of bacteria on the floor, choose the clean above-mentioned number of bacteria of handling through spray composite of the present invention of handling with alcohol everywhere.

The present invention also is one and is used for judging whether the employee has been subjected to good protection whether press effective checking and monitoring reagent box and method that the correct using method of this product is used.This test kit comprises the bottle that a valve protection cap can be taken away, and is a core below the valve protection cap, as a big marking pen.The bottle the inside is the material of blue dyeing soluble in water (Viola crystallina).As long as use product of the present invention and dry by instructing, the tip of applicator just can contact any local surfaces, and moves 1 to 1  inch along the surface.The local 5-8 of contact can stay blueness after second, wipes with a thin paper then.If this product correctly is coated on the skin, should be unable to stay color.If blue vestige still exists, then surface skin is not coated skin care product.Can be applied to any part on hand after this test is reused at random, therefore not need to consider that being coated onto same zone by preordering method just can carry out the test of back, be coated onto the back of the hand and be coated onto the back of the hand, finger side, thumb bottom Anywhere.Fig. 9-14 for example understands how to carry out violet staining with this product.

Figure 16 for example understands this product after having formed protective layer on the skin, and discharging more than the product by former technology manufacturing, multiple medicines thing (being hydrocortisone in this example) sees through skin.

Under the situation that the specific embodiment of the present invention is illustrated and described, different modifications and composition are also without departing from the spirit and scope of the present invention.Though the present invention has described relevant with health care and cooking environments part, disclosed composition may be used in any environment.The Equivalent of modification of the present invention, combination and composition of the present invention and system is included within the scope of the present invention also claimed.

Claims (14)

1, a kind of system that monitors pathogenic micro-organism and stimulator, this system comprises step:

A. wash skin with antibacterial perfumed soap of washing one's hands;

B. use the antibiotic skin-care washing lotion; With

C. at the crust in the zone antimicrobial surface cleaner of spraying.

2, be used for detecting the user and whether abide by antimicrobial checking and the monitoring reagent box that the using method of perfumed soap or skin care washing lotion uses of washing one's hands, this test kit comprises: the removable bottle of valve protection cap, core and be diluted in dyestuff in the water.

3, a kind of emulsion composition of cleaning skin, said composition comprises:

Mass percent is approximately the pure water of 85-99.5%, and mass percent is approximately the N of 0.25-5%, and N-pair-(2-OH-ethyl) laurylamide, mass percent is approximately the C of 0.05-3% _14-16The semi-annular jade pendant acid sodium of paraffin, mass percent is approximately the polyvinyl alcohol bay ether semi-annular jade pendant acid sodium of 0.03-3.5%, mass percent is approximately the polyquaternium-7 of 0.015-2.5%, mass percent is approximately the chlohexidine of 0.25-4%, and mass percent is approximately the phenol that 0.05-1.75% antioxidant and mass percent are approximately 0.015-1.75%.

4, emulsion composition as claimed in claim 3, it also contains the vegetable fatty acid of mass percent for about 0.02-1.5%.

5, a kind of emulsion composition that is topically applied to skin, said composition comprises: mass percent is approximately the pure water of 80.0-99.5%, mass percent is approximately waxy starches or the polysaccharide of 0.5-15.0%, mass percent is approximately Vaseline or other mineral oil of 0.125-12.0%, mass percent is approximately other plant or vegetable oil or the extract of 0.01-8.0%, mass percent is approximately the viscosity stabilizer of 0.01-3.0%, mass percent is approximately the viscosity intensifier of 0.01-3.0%, mass percent is approximately antioxidant or the stablizer of 0.01-5.0%, the magnesium chloride of the 0.01-1.0% that mass percent is approximately, mass percent is approximately the benzoin methyl acid phenolic ester of 0.01-1.0%, mass percent is approximately the Citricidal of 0.01-3.0%, mass percent is approximately the Benzalkonium Chloride 80(BKC80) of 0.01-2.0%, and mass percent is approximately the 3N-butyl PO of 0.01-1.0% ₄

6, emulsion composition as claimed in claim 5, it also contains the phenylformic acid of mass percent for about 0.01-1%.

7, emulsion composition as claimed in claim 5, it also contains the boric acid of mass percent for about 0.01-1%.

8, emulsion composition as claimed in claim 5, it also contains the benzoyl peroxide of mass percent for about 0.05-3%.

9, a kind of spray composite that is used in the surface, said composition comprises:

Mass percent is approximately the pure water of 85.0-99.5%, water glass, mass percent is approximately the Nonoxynol-9 of 0.02-2.5%, mass percent is approximately the butyl glycol ether of 0.05-5.5%, mass percent is approximately the Barquat 50 of 0.05-3.5%, and mass percent is approximately the Barlox 12 of 0.05-3.0%.

10, emulsion composition as claimed in claim 9, it also contains mass percent and is about 0.05-3.0% benzoyl peroxide.

11, be used for judging whether the human consumer abides by antimicrobial checking and the monitoring method that the using method of perfumed soap or emulsion for skin care uses of washing one's hands, the method comprising the steps of: allow the tip of the applicator that contains dyestuff contact any local surfaces, tip according to applicator is moved 1 to 1.5 inch along above-mentioned surface, dry described dyestuff 5-8 second, with a described surface of thin paper wiping, whether observe at the coloured vestige in above-mentioned surface.

12, method as claimed in claim 11, wherein said local surfaces is a staff.

13, method as claimed in claim 11, wherein said dyestuff is a blue dyes.

14, method as claimed in claim 13, wherein said blue dyes is a Viola crystallina.

Images