CN1781518A - Medicinal composition for treating milk cow mammitis and its preparing method - Google Patents
Medicinal composition for treating milk cow mammitis and its preparing method Download PDFInfo
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- CN1781518A CN1781518A CN 200410073373 CN200410073373A CN1781518A CN 1781518 A CN1781518 A CN 1781518A CN 200410073373 CN200410073373 CN 200410073373 CN 200410073373 A CN200410073373 A CN 200410073373A CN 1781518 A CN1781518 A CN 1781518A
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Abstract
The medicine composition for treating mammitis of milk cow is prepared with Gansu sage, forsythia, Chinese goldthread and honeysuckle, and through extraction and separation to obtain extracts, and dissolving in propylene glycol and injection water to prepare injection. It has powerful functions of resisting bacteria, diminishing inflammation and promoting blood circulation to disperse blood clots, and can cure mammitis of milk cow quickly.
Description
Technical field
The invention belongs to a kind of treatment mammitis of cow pharmaceutical composition and this preparation of drug combination method thereof.
Background technology
Mammitis of cow is one of three big important diseases of milch cow.The annual economic loss that causes because of mammitis of cow reaches multi-million dollar in the whole world, also influences the quality of milk output and milk product thereof simultaneously.For a long time,, mainly both at home and abroad adopt antibiotic and chemical synthetic drug, but above outstanding problem shows as medicine residual in milk to the treatment of mammitis of cow; Secondly, life-time service can cause that the cow breast flora produces serious drug resistance and to consequences such as public health health work the mischief.The tradition of China's useful since ancient times treatment by Chinese herbs domestic animal mastitis, but all be confined to the route of administration that herbal mixture is fried or pulverizing gavages.
Summary of the invention
Based on the deficiencies in the prior art, purpose of the present invention aims to provide a kind of pharmaceutical composition and this preparation of drug combination technology for the treatment of mammitis of cow.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of pharmaceutical composition for the treatment of mammitis of cow is with crude drug Salvia przewalskii, Fructus Forsythiae, Rhizoma Coptidis, Flos Lonicerae; Adjuvant propylene glycol, water for injection are made.
Purpose of the present invention can also be achieved through the following technical solutions:
A kind of pharmaceutical composition for the treatment of mammitis of cow is that adjuvant propylene glycol, water for injection are made with Salvia przewalskii, Fructus Forsythiae, Rhizoma Coptidis, Flos Lonicerae extraction separation thing,
Calculate with 1000 milliliters of medicinal liquids, component is:
| The fat-soluble extraction separation thing of TANSHINONES extraction separation thing Fructus Forsythiae Rhizoma Coptidis water solublity extraction separation thing Flos Lonicerae water solublity extraction separation thing in the Salvia przewalskii | 0.40～0.44g/1000ml 50.0～55.0g1000/ml 25.0～27.5g/1000ml 25.0～27.5g/1000ml | In TANSHINONES extraction separation thing in ester dissolubility total extract in the water solublity total extract in the water solublity total extract |
| The distilled water propylene glycol | 70％ 30％ | By volume is calculated by volume and is calculated |
Pharmaceutical composition extraction, separation, the preparation technology of treatment mammitis of cow:
1. with the 1kg Salvia przewalskii, fine powder is broken, adds the 1500ml dichloromethane, adds 500ml95% ethanol then, and merceration 3 hours filters, and collects and gets filtrate.Method is extracted 3 times according to this, merge extractive liquid,, and distillating recovering solvent gets liposoluble extract.Extract restrains with silica gel 500 and mixes, and inserts silicagel column then, uses petroleum ether: methanol=be solvent at 80: 20, and eluting is collected the red stripes eluent between 36h～100h, reclaims solvent, gets solid constituent, dry for standby under 60 ℃ of conditions;
2. with the 1kg Fructus Forsythiae, fine powder is broken, adds the 2000ml dichloromethane, and merceration is 4 hours under the room temperature, filters, and collects filtrate.Method is extracted 3 times according to this, merge extractive liquid,, and the heating distillating recovering solvent gets fat-soluble solid constituent.Extract mixes with silica gel 500 gram, and upper prop then use petroleum ether: ethyl acetate=be solvent at 90: 10, and eluting is collected eluent, reclaims solvent, and it is standby to get solid constituent;
3. with the 1kg Rhizoma Coptidis, coarse pulverization adds the 3000ml ordinary water, and 95 ℃ of heating extraction 2h filter, and method is extracted 3 times according to this, and merge extractive liquid, carries out precipitate with ethanol with 85% ethanol then, the centrifuging and taking supernatant.Continuous 3 times, reclaim supernatant, the second alcohol and water is reclaimed in distillation, gets the solid constituent extract.Extract restrains with silica gel 500 and mixes, and column chromatography is used methanol: ethyl acetate=be solvent at 75: 25, and eluting is collected eluent, reclaims solvent, gets solid constituent, dry for standby;
4. Flos Lonicerae 1kg adds the 2500ml ordinary water, and 95 ℃ of heating extraction 2h cross leaching filtrate, and method is extracted 3 times according to this, and merge extractive liquid, carries out precipitate with ethanol with 85% ethanol then, crosses the leaching supernatant.Continuous 3 times, merge supernatant, the second alcohol and water is reclaimed in distillation, gets the solid constituent extract.Extract restrains with silica gel 500 and mixes, and column chromatography is used methanol: ethyl acetate=be solvent at 60: 40, and eluting is collected eluent, reclaims solvent, gets solid constituent, and dry back is standby;
5. calculate according to the preparation of 1000ml medicinal liquid, accurately take by weighing TANSHINONES extraction separation thing 0.40～0.44g and Fructus Forsythiae extraction separation thing 50.0～55.0g, add propylene glycol 300ml, stir 1h according to constituent content in the prescription.Get and add Rhizoma Coptidis, each 25.0～27.5g of Flos Lonicerae extraction separation thing, use the 700ml dissolved in distilled water, stir 30min, partially mixed with the propylene glycol dissolving then, be settled to 1000ml with distilled water, stir 30min, G3 hangs down and holds funnel filtration, bottling, every bottle of 50ml, with 10 pounds of pressure high-temperature sterilization 30min, promptly get the purpose product.
The physicochemical property of pharmaceutical composition:
Appearance luster: pink clear liquid.
Proportion: 1.08
PH value: 5.5 ± 1.0
The beneficial effect of advantage of the present invention and generation is:
Pharmaceutical composition of the present invention belongs to the injection of pure treatment by Chinese herbs mammitis of cow.It is raw material that medicine adopts natural plant, and to each single the effective elements of the medicine process extraction separation, collect medicine and have active chemical compound of particular organisms and compound group, carry out prescription then, through distinctive dissolution with solvents, cooperate and make the medicine for treatment thing that meets the injection standard.It overcomes traditional antibiotic and the chemicals problem such as residual in milk product, bring into play the powerful effect of Chinese herbal medicine anti-inflammation, blood circulation promoting and blood stasis dispelling in disease treatment simultaneously, and make the new medicament of the treatment mammitis of cow that is suitable for clinical use with brand-new preparation technology.The present invention also changed traditional compound recipe fried, pulverize and to gavage or the Therapeutic Method of the combination of Chinese and Western medicine, simple and convenient, nontoxic, fairly obvious for treatment mammitis of cow effect.
The specific embodiment
Embodiment 1
Calculate according to the preparation of 1000ml medicinal liquid, take by weighing TANSHINONES extraction separation thing 0.42g and Fructus Forsythiae extraction separation thing 52.5g, add propylene glycol 300ml, stir 1h according to constituent content precision in the prescription.Add Rhizoma Coptidis, each 26.25g of Flos Lonicerae extraction separation thing again, use the 700ml dissolved in distilled water, stir 30min, partially mixed with the propylene glycol dissolving then, be settled to 1000ml with distilled water, stir 30min, G3 hangs down and holds funnel filtration, bottling, every bottle of 50ml, with 10 pounds of steam high-temperature sterilization 30min, promptly get the purpose product.This product is preserved under room temperature, lucifuge, shady and cool condition, and effect duration is 2 years.
Embodiment 2
Test method: 40 Kunming white mouses, body weight 20 ± 2g is equally divided into 5 groups, and 8 every group, male and female half and half, the medicament that per os gavages embodiment 1 preparation carries out acute toxicity test.
Result of the test, after high dose group mice this product was irritated stomach, mice occurred squeezing for sleeping in, movement disorder, does not eat symptoms such as rapid breathing; Half an hour, the symptom of body appearred turning round in the rear section, and the part health is shaken, and is all dead in the 12h, the 4th group in one day dead three, second day dead two.Mice to death is cutd open inspection, and the stomach enlargement has yellow liquid, the duodenum chap, and full of liquid, the kidney enlargement, the splenomegaly of part mice, conscience lung and remaining tissue are normal, do not have other macroscopic pathological changes.Yellow liquid is less in the dead mice harmonization of the stomach of the low dose group duodenum, and duodenum is chap still, the enlargement of spleen part stomach, and the kidney enlargement, conscience lungs etc. are normal, do not have other macroscopic variations.
The result of the test statistics sees Table 1, as calculated, and the oral LD of this product mice
50Be the 36.80g/kg body weight.LD
5095% credible 32.12～41.47g/kg body weight that is limited to.According to toxicology poisonous substance grade scale, these product should be actual nontoxic (in the toxicologic study, the oral LD of the mice of medicine
50During more than or equal to 10g, be regarded as actual nontoxic).
The oral this product acute toxicity test of table 1 mice statistical result
| Grouping | Dosage (ml/kgB.W.) | Number of animals (only) | Death toll (only) | Mortality rate (%) |
| 1 | 19.60 | 8 | 0 | 0 |
| 2 | 24.13 | 8 | 1 | 12.5 |
| 3 | 29.67 | 8 | 3 | 37.5 |
| 4 | 36.50 | 8 | 5 | 62.5 |
| 5 | 45.0 | 8 | 8 | 100.0 |
Embodiment 3
The antiinflammatory test of pharmaceutical composition of the present invention
Test method: adopt caused by dimethylbenzene xylene white mice auricle edema method.With body weight is that the white mice of 20 ± 2g is divided into three groups at random, i.e. medicine group, medicine matched group and blank group, eight every group.Medicine is used by embodiment 1 method and is prepared medicament, and dosage is 0.25ml/; Medicine matched group medication hydrocortisone, dosage are 0.1ml/ only (concentration is 1mg/m1); Intraperitoneal injection, continuous use 4 days, every day 1 time.The blank group does not process.8h after the last administration under slight etherization, is coated in the two sides, left ear front and back of respectively organizing white mice, the shared 0.05ml of every Mus with causing scorching liquid dimethylbenzene.Be coated with back 1 hour and put to death mice.Two ears about every Mus is cut with the round auricle that the card punch of diameter 5mm is laid same position, are weighed on analytical balance.The weight that the weight of the left auricle of every Mus deducts the auris dextra sheet is the swelling degree.The swelling degree of matched group and medicine group, control drug group is carried out statistical analysis.
Table 3 this product xylol causes the influence of white mice ear swelling
| Group | Number of animals (only) | Dosage (ml/ only) | Weightening finish (mg) (X ± SD) | Suppression ratio (%) |
| Blank group medicine matched group this product group | 8 8 8 | - 0.1 0.25 | 13.05±2.30 9.12±3.58* 9.22±2.23* | - 34％ 29％ |
Annotate:
*P＜0.05, significant difference
What antiinflammatory was tested the results are shown in Table 3.The result shows that the antiphlogistic effects of this product and hydrocortisone is suitable, and the significant difference (p＜0.05) of comparing with the blank group has good antiinflammatory action.
Embodiment 4
Pharmaceutical composition antagonism main pathogen of bovine mastitis of the present invention test
Test method: get 6 kinds of pathogen that the inclined-plane is preserved, getting a platinum erbium respectively is inoculated in and trains 24h in the ordinary broth, after smear for microscopic examination is defined as single culture, bacterium liquid adopts doubling dilution, to dilute 5 times by the medicament of embodiment 1 method preparation with water for injection, matched group penicillin content is 50,000 units per ml.Adopt cup-plate method, in the culture dish that is covered with the plain agar culture medium, add experimental bacteria 0.1ml, the L rod is applied, and 6 sterilizing stainless steel pipes (Φ 6mm) are evenly placed wherein, adds in every tubule to be subjected to test solution 0.3ml, behind 37 ℃ of cultivation 24h, measure antibacterial circle diameter D (mm).
Antibacterial tests result shows, this product all has good fungistatic effect to the main malignant bacteria streptococcus agalactiae of mastitis, streptococcus dysgalactiae, gold-coloured staphylococci, escherichia coli, streptococcus uberis and klebsiella, and the bacteriostasis of above antibacterial all is better than matched group medicine penicillin.
Table 4 this product is to the inhibition effect antibacterial circle diameter of mammitis of cow The main pathogenic fungi: mm
| Antibacterial | This product (mm) | Penicillin matched group (mm) |
| Streptococcus agalactiae 32327 streptococcus dysgalactiaes 2531 gold-coloured staphylococcis 84184 escherichia coli 88168 streptococcus uberises 78170 Crays uncle 219 | 17.4 18.9 14.8 18.1 12.4 10.8 | 10.2 9.5 7.8 2.1 9.1 5.5 |
Embodiment 5
Clinical testing data of the present invention
Select to suffer from lactation period clinic mastitis both mammary gland red and swollen heat pain appears, milk is observed and the breast district unusual, that milk yield descends occurred as the therapeutic test case, totally 1960 morbidity breast districts, wherein acute mastitis breast district is 1260; 610 in subacute type mastitis breast district; 90 in chronic mammitis breast district.1260 breast districts are divided into two groups at random, 1. test group: 1120 breast districts, use this product treatment.2. matched group: 840 breast districts, use the penicillin and streptomycin treatment.
Test method: earlier the indoor milk of ill breast is extracted, behind 75% cotton ball soaked in alcohol sterilization nipple, adopted the milk duct injection method, medicament by the preparation of embodiment 1 method once injects 50ml, massages breast then gently, and morning and afternoon respectively once, 3 days is a course of treatment, 2 courses of treatment of logotype.Matched group adds streptomycin 1g with penicillin 800,000 units, is dissolved in the 50ml aquesterilisa, injects for 1 time as stated above, and the course of treatment is with the medicine group.
According to treatment back clinical symptoms, lactogenic etc. curative effect is divided into:
1. clinical recovery: the breast red and swollen heat pain disappears, the milk outward appearance is normal, recover normal lactation;
2. effective: breast inflammation obviously alleviates, the milk outward appearance is normal substantially, milk yield obviously increases;
3. invalid: milk outward appearance and milk yield can not be recovered normally after the treatment of two courses of treatment, with no much variation persons before the treatment.
Result of the test: the clinical mastitis therapeutic effect of milch cow is shown, this medicament and mycillin group treatment mastitis effective percentage, cure rate the highest with this product, especially to acute and subacute mastitis is the most remarkable with this product.Chronic mammitis this product is also had good therapeutic effect, and mycillin is not because tradition life-time service clinically has effect substantially.This product just can reduce the temperature of breast rapidly after 1～2 medication, alleviate the swelling degree, and burning pain alleviates.Ordinary circumstance just can make breast become soft next course of treatment, and it is normal that milk and milk production are recovered.
Table 5 this product treatment milch cow clinic mastitis effect
| Medicine | Type | Breast district number | Cure rate/% | Effective percentage/% | Inefficiency/% | Total effective rate/% | Total/% |
| This product | Acute subacute chronic | 720 340 60 | 81.9 82.4 50.0 | 12.5 14.7 16.7 | 5.6 2.9 33.3 | 94.4 97.1 66.7 | 93.8 |
| Penicillin and streptomycin | Acute subacute chronic | 540 270 30 | 56.8 70.4 0 | 16.7 14.8 0 | 37.5 14.8 0 | 62.5 85.2 0 | 79.3 |
The using method of treatment mammitis of cow
The cow breast perfusion, each morbidity breast each 50ml in district, used 3～5 days continuously at every day 1 time.
Claims (3)
1, a kind of pharmaceutical composition for the treatment of mammitis of cow is characterized in that with Chinese herbal medicine be Salvia przewalskii, Fructus Forsythiae, Rhizoma Coptidis, Flos Lonicerae; Adjuvant is that propylene glycol, water for injection are made.
2, according to claims 1 described a kind of pharmaceutical composition for the treatment of mammitis of cow, it is characterized in that adjuvant propylene glycol, water for injection are made with Salvia przewalskii, Fructus Forsythiae, Rhizoma Coptidis, Flos Lonicerae extraction separation thing,
Calculate with 1000 milliliters of medicinal liquids, component is:
TANSHINONES extraction separation thing 0.40～0.44g/1000ml is in TANSHINONES extraction separation thing in the Salvia przewalskii
The fat-soluble extraction separation thing of Fructus Forsythiae 50.0～55.0g1000/ml is in ester dissolubility total extract
Rhizoma Coptidis water solublity extraction separation thing 25.0～27.5g/1000ml is in the water solublity total extract
Flos Lonicerae water solublity extraction separation thing 25.0～27.5g/1000ml is in the water solublity total extract
Distilled water 70% by volume is calculated
Propylene glycol 30% by volume is calculated
3, as claims 1 or 2 described a kind of preparation of drug combination technologies for the treatment of mammitis of cow, its separating step is:
1. with the 1kg Salvia przewalskii, fine powder is broken, adds the 1500ml dichloromethane, adds 500ml95% ethanol then, and merceration 3 hours filters, and collects and gets filtrate.Method is extracted 3 times according to this, merge extractive liquid,, and distillating recovering solvent gets liposoluble extract.Extract restrains with silica gel 500 and mixes, and inserts silicagel column then, uses petroleum ether: methanol=be solvent at 80: 20, and eluting is collected the red stripes eluent between 36h～100h, reclaims solvent, gets solid constituent, dry for standby under 60 ℃ of conditions;
2. with the 1kg Fructus Forsythiae, fine powder is broken, adds the 2000ml dichloromethane, and merceration is 4 hours under the room temperature, filters, and collects filtrate.Method is extracted 3 times according to this, merge extractive liquid,, and the heating distillating recovering solvent gets fat-soluble solid constituent.Extract mixes with silica gel 500 gram, and upper prop then use petroleum ether: ethyl acetate=be solvent at 90: 10, and eluting is collected eluent, reclaims solvent, gets the solid constituent drying for standby;
3. with the 1kg Rhizoma Coptidis, coarse pulverization adds the 3000ml ordinary water, and 95 ℃ of heating extraction 2h filter, and method is extracted 3 times according to this, and merge extractive liquid, carries out precipitate with ethanol with 85% ethanol then, the centrifuging and taking supernatant.Continuous 3 times, reclaim supernatant, the second alcohol and water is reclaimed in distillation, gets the solid constituent extract.Extract restrains with silica gel 500 and mixes, and column chromatography is used methanol: ethyl acetate=be solvent at 75: 25, and eluting is collected eluent, reclaims solvent, gets solid constituent, dry for standby;
4. Flos Lonicerae 1kg adds the 2500ml ordinary water, and 95 ℃ of heating extraction 2h cross leaching filtrate, and method is extracted 3 times according to this, and merge extractive liquid, carries out precipitate with ethanol with 85% ethanol then, crosses the leaching supernatant.Continuous 3 times, merge supernatant, the second alcohol and water is reclaimed in distillation, gets the solid constituent extract.Extract restrains with silica gel 500 and mixes, and column chromatography is used methanol: ethyl acetate=be solvent at 60: 40, and eluting is collected eluent, reclaims solvent, gets solid constituent, and dry back is standby;
5. calculate according to the preparation of 1000ml medicinal liquid, accurately take by weighing TANSHINONES extraction separation thing 0.40～0.44g and Fructus Forsythiae extraction separation thing 50.0～55.0g, add propylene glycol 300ml, stir 1h according to constituent content in the prescription.Get and add Rhizoma Coptidis, each 25.0～27.5g of Flos Lonicerae extraction separation thing, use the 700ml dissolved in distilled water, stir 30min, partially mixed with the propylene glycol dissolving then, be settled to 1000ml with distilled water, stir 30min, G3 hangs down and holds the funnel filtration, bottling with 10 pounds of pressure high-temperature sterilization 30min, promptly gets the purpose product.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100733738A CN100384444C (en) | 2004-11-30 | 2004-11-30 | Medicinal composition for treating milk cow mammitis and its preparing method |
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|---|---|---|---|
| CNB2004100733738A CN100384444C (en) | 2004-11-30 | 2004-11-30 | Medicinal composition for treating milk cow mammitis and its preparing method |
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| CN1781518A true CN1781518A (en) | 2006-06-07 |
| CN100384444C CN100384444C (en) | 2008-04-30 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112618598A (en) * | 2020-12-31 | 2021-04-09 | 广西大学 | Preparation and quality detection of traditional Chinese medicine compound Xiaolinian for preventing and treating cow mastitis |
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| CN1217686C (en) * | 2003-04-01 | 2005-09-07 | 赵林 | Preparation method of dairy cattle mammitis anhinflammation powder |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112618598A (en) * | 2020-12-31 | 2021-04-09 | 广西大学 | Preparation and quality detection of traditional Chinese medicine compound Xiaolinian for preventing and treating cow mastitis |
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Assignee: Hubei Wudang Animal Pharmaceutical Co., Ltd. Assignor: Lanzhou Inst. of Animal Husbandary & Veterinary Medicine, Chinese Academy of Agr Contract record no.: 2011420000091 Denomination of invention: Combination of lmedication for treatig mastitis of cow and preparation method Granted publication date: 20080430 License type: Exclusive License Open date: 20060607 Record date: 20110517 |
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