CN1777418B - Composition for slimming - Google Patents
Composition for slimming Download PDFInfo
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- CN1777418B CN1777418B CN2004800107268A CN200480010726A CN1777418B CN 1777418 B CN1777418 B CN 1777418B CN 2004800107268 A CN2004800107268 A CN 2004800107268A CN 200480010726 A CN200480010726 A CN 200480010726A CN 1777418 B CN1777418 B CN 1777418B
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- theanine
- fat
- catechin
- carnitine
- caffeine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/06—Preparations for care of the skin for countering cellulitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
Abstract
The present invention relates to a composition for slimming, more particularly, to a slimming composition containing theanine and at least one selected from the group consisting of caffeine, genistein, L-carnitine and catechin. The composition of the present invention contains theanine and each of caffeine, genistein, L-carnitine, catechin or mixtures thereof, and has properties of decomposing fats, hydrolyzing lipid and removing cellulites.
Description
Technical field
The invention relates to a kind of Weight reducing compound, more specifically about a kind of Weight reducing compound that contains theanine (theanine) and be selected from least a material in the group of being formed by caffeine, genistein (genistein), L-carnitine and catechin (catechin).Compositions of the present invention contains one or more in theanine and caffeine, genistein, L-carnitine and the catechin, have reduce fat, the characteristic of hydrolyze lipids and removal liparitosis.
Background technology
Human body has about 20000000000 adipose cells that store in vivo and release energy.Energy storage and release in vivo has complicated mechanism, and when energy in large supply during in consumption, energy will store as neutral fat (lipid) in adipose cell (lipocyte); When the needs energy, fat will be hydrolyzed into as the fatty acid of energy and glucose.Broken and unnecessary energy accumulation when getting up when the energy balance of above-mentioned mechanism, obesity will occur, adipose cell increases or the number of adipose cell increases as a result.
It is reported that approximately the modern of 30-40% suffers from obesity, and because obesity often is accompanied by infirmities of age such as hypertension, hyperlipidemia, arteriosclerosis, heart disease, diabetes etc., so the treatment of obesity is most important.In addition, people pay close attention to the treatment of obesity and the maintenance of the good health and the bodily form is not only for health, also for beauty.For beauty, to can effectively remove unnecessary subcutaneous fat or improve the tenacity of skin or the demand of elastic fat-reducing and anti-liparitosis cosmetics also growing.
Because the accumulation of fat and metabolic waste thereof forms liparitosis in skin and subcutaneous fat, liparitosis makes pachylosis must look like orange peel.Even obesity is not the immediate cause that produces liparitosis, but liparitosis will increase when the volume of adipose cell or number become big, with steatolysis in the adipose cell and removal, is very effective for keeping the good bodily form and skin therefore.
Therefore, for healthy or beautiful, people have carried out a large amount of researchs about various treatment of obesity methods.The method of conventional therapy obesity comprises bantingism, exercise regimen, surgical operation therapy or the pharmacotherapy that reduces the energy intake or increase energy consumption.Yet because these methods can not solve problem of obesity fully and have serious adverse, therefore effectiveness and safety still are difficult to be guaranteed.In addition, for beauty, the treatment of obesity not only will be removed fat, should also be noted that and improve skin, so said method is still inadequate.Therefore, need badly find have with existing material type like or the better effect and the novel substance of being free from side effects.
Consider the cause of disease of obesity and the disease of bringing out thereof, it is more important than reducing body weight simply to reduce body fat.Therefore, need find a kind of nonessential steatolysis or incendiary method that promotes cylinder accumulation.
The present inventor studies the safe material that searching can be promoted steatolysis or hydrolysis and promote fat combustion, and finds that finally theanine, caffeine, genistein, L-carnitine and catechin have effective hydrolyze lipids, remove liparitosis and lipolytic performance; And find that theanine, caffeine, genistein, L-carnitine and catechin can help health to keep better elastic and tenacity.
Summary of the invention
The invention provides the new purposes of theanine, described theanine can promote the decomposition of neutral fat and can promote the expression of β 3 adrenoceptors and help fat burning; The new purposes of caffeine, described caffeine can suppress the expression of phosphodiesterase and promote fatty decomposition, and described phosphodiesterase is a kind of enzyme that fat decomposes in adipose cell that suppresses; The new purposes of genistein, described genistein can promote the decomposition and the burning of fat; The new purposes of L-carnitine mixture, described L-carnitine mixture can promote the lipolytic function of genistein; The new purposes of catechin, described catechin can suppress the differentiation of adipose cell (lipocyte).
The present invention also provides a kind of external use to lose weight compositions, and said composition contains at least a material in theanine and caffeine, genistein, L-carnitine and the catechin.Compositions provided by the invention have reduce fat, hydrolyze lipids, removal liparitosis and the characteristic that reduces the pachylosis that causes by liparitosis, thereby can strengthen or recover the tenacity and the elasticity of skin.
In addition, the invention provides the method for removing body fat, this method reduces fat by use above-mentioned Weight reducing compound on skin.
In order to realize purpose of the present invention, Weight reducing compound of the present invention contains theanine and is selected from least a material in the group of being made up of caffeine, genistein, L-carnitine and catechin, and said composition is promoted the decomposition of fat by the metabolism that promotes adipose cell.
To describe the present invention in detail below.
Term " fat-reducing " is meant and suppresses and alleviate obesity and reduce liparitosis and the bodily form is improved, makes skin become tough and tensile, smooth, flexible.
Theanine is a kind of aminoacid that presents the peculiar taste of green tea, it is reported, when a people takes theanine, α ripple (α-ray) will increase, make people's body and mind loosen, be emotionally stable (Nippon Nogeikagaku Kaishi.72 (2), 153-157,1998).Theanine of the present invention comprises L type and synthetic L-theanine, D-theanine and the DL-theanine that extracts from green tea, and the theanine of any kind as used herein.
As the positive control of lipoclasis accelerant and celebrated caffeine is a kind of methylxanthine that demonstrates steatolysis character, methylxanthine can suppress the expression of phosphodiesterase, the cyclic adenosine monophosphate (cAMP) in the increase cell, described phosphodiesterase is the important substance (Astrup in the steatolysis, A etc., Am J.Clin.Nutr.51:759,1990).
Genistein is a kind of isoflavone that contains in the Semen sojae atricolor of being everlasting, and is and the similar phytohormone of the estrogen with various physiologically actives.It is reported that genistein has lipid metabolism (J.Steroid Biochem Mol Bio1.75 (4-5): 265-71,2000) and reduction blood cholesterol levels (J.Nutr.Jan in the control lipocyte; 126 (1): 43-50,1996) characteristic.
The L-carnitine is a synthetic essential nutrients in liver or kidney, generally contains in food especially red meat.It is reported that when the L-carnitine was not enough, the fatty acid concentration in the mitochondrion will reduce, therefore the growing amount of energy will reduce, and the L-carnitine has multiple function, as (the Robert Crayhon such as function of anti-aging effects, blood fat reducing, enhancing heart, M.S., Camitine miracle).
Catechin is an important component of green tea, has multiple pharmacological effect, as antioxidation, antitumaous effect or inhibition heart disease.So far Bao Dao multiple catechin comprises (+) catechin (C), (-) epicatechin (EC), (-) epicatechin-3-gallate (EGCG), (+) epicatechin (EGC), (-) epicatechin gallate (ECG).Catechin of the present invention is extracted from green tea, and its main component includes but not limited to EC, EGC, EGCG, ECG.
The method of extracting effective ingredient of the present invention does not limit the various known method in the use this area that can have no to limit.
Compositions of the present invention contains one or more in theanine and caffeine, genistein, L-carnitine and the catechin, has the good efficacy of neutral fat in the cell that reduces fat.More specifically, compositions of the present invention promotes fatty decomposition by the triglyceride in the lipocyte (adipose cell) being hydrolyzed into free fatty and glycerol.This is because compositions of the present invention can be promoted the expression of β 3 adrenoceptors in the 3T3-L1 cell that is divided into lipocyte, thereby strengthen and keep the hydrolysis of triglyceride, and said composition also has the expression of the decomposition of enhancement and fat or the relevant enzyme that burns.
Therefore, when being applied in the compositions that contains at least a material in theanine and caffeine, genistein, L-carnitine and the catechin of the present invention on the skin, said composition will help to make health to become solid, smooth, flexible by optionally removing subcutaneous fat.
In addition, compositions of the present invention has the effect of accumulation of fat in good inhibition adipose cell (lipocyte) differentiation and the adipose cell.It is the increase that compositions of the present invention can suppress adipose cell volume and number.This is because compositions of the present invention can reduce the activity of glycerol-3-phosphate ester dehydrogenase (GPDH) in the 3T3-L1 cell that is divided into lipocyte, and described GPDH is the marked member of adipose cell differentiation.Therefore, when being administered to the compositions that contains at least a material in theanine and caffeine, genistein, L-carnitine and the catechin of the present invention on the skin, just can pass through to suppress the generation and the increase of adipose cell, thereby suppress the increase of body fat effectively.
Except reducing fat, can also increase on the liparitosis position that causes by compositions of the present invention being administered to by adipose cell, with the topical composition of compositions of the present invention as anti-liparitosis, be used for treating rough skin, make skin become tough and tensile, smooth and flexible.Especially the composition owing to said composition extracts from green tea and Semen sojae atricolor, so it can not cause side effect or injury to skin.
Except decomposing and remove the fat of excess accumulation in mature fat cell, compositions of the present invention also helps combustion of free fatty or suppresses obesity.With traditional only pay close attention to a characteristic as the differentiation adipose cell promote fat-splitting method or compositions opposite, the fat that compositions of the present invention can reduce fat and generate in the cell, and can thoroughly remove the by-product of decomposition and prevent that fat from accumulating again, thereby a kind of method and composition of new direct prevent obesity is provided.
Can use component theanine, caffeine, genistein, L-carnitine and the catechin of the method mixing present composition of any routine, those skilled in the art can easily change these methods.In addition, can use some additives unqualifiedly, mix the additive that is more prone to such as making.
In the present invention, at least a amount of substance in theanine and caffeine, genistein, L-carnitine and the catechin preferably accounts for the 0.0001-20 weight % of described composition total weight, but is not limited to this.
Topical composition of the present invention contains at least a material in theanine and caffeine, genistein, L-carnitine and the catechin, therefore, in the time of on being administered to skin, said composition can reduce subcutaneous fat makes the bodily form slender, and has by decomposing subcutaneous fat and remove liparitosis and make the tough and tensile effect of the bodily form.
The external preparation that contains the compositions of at least a material in theanine and caffeine, genistein, L-carnitine and the catechin of the present invention is not limited to that said composition is used for decomposing and burning fat, makes the tough and tensile resilient situation of skin.For example, except comprising washing liquid, ointment, gel, frost, paster, spray etc., described preparation also comprises skin soft agent, nutritional solution, nutrition washing liquid, massage cream, nourishing cream, Liniment, gel, adhering skin type preparation.
In above-mentioned skin application formulations, except effective ingredient theanine, caffeine, genistein, L-carnitine and catechin, those skilled in the art can select and add other any appropriate ingredients.By adding appropriate ingredients, can realize cooperative effect.
Description of drawings
Fig. 1 is the effect of neutral fat (triglyceride) is decomposed in expression from the composition theanine in the green tea a chart;
Fig. 2 is the chart of expression from the effect of the control of the composition theanine in green tea β 3 adrenoceptors expression,
A: contrast
B: handle with 0.005% caffeine
C: handle with 0.005% theanine;
Fig. 3 is expression stops the effect of adipose cell differentiation from the composition catechin in the green tea a chart;
Fig. 4 is the chart that expression theanine and catechin suppress the cumulative synergistic experimental result of neutral fat in the adipose cell;
Fig. 5 is that expression theanine, caffeine, genistein and L-carnitine are promoted the chart of fat-splitting synergistic experimental result;
Fig. 6 is the chart that expression Weight reducing compound of the present invention makes the speed of subcutaneous fat minimizing;
Fig. 7 is that the chart that skin firmness is improved is used after the Weight reducing compound that contains theanine, caffeine, genistein, L-carnitine and catechin of the present invention in expression.
The specific embodiment
Describe the present invention in more detail below by embodiment and EXPERIMENTAL EXAMPLE, but scope of the present invention is not limited to this.Describing these EXPERIMENTAL EXAMPLE and EXPERIMENTAL EXAMPLE is in order to demonstrate the invention, those skilled in the art know that scope of the present invention is not limited to these embodiment and EXPERIMENTAL EXAMPLE.
Theanine used in following examples and the EXPERIMENTAL EXAMPLE is available from Taiyokagaku (Japan), and caffeine, genistein and L-carnitine are available from Sigma (U.S.).
The extraction of preparation embodiment 1 catechin
2 kilograms of green teas were soaked 5 hours in 10 liters of 80 ℃ of water, obtain extracting solution and collect gained solution.In addition, filtering residue was soaked 3 hours in 5 liters of 80 ℃ of water, obtain solution once more, and twice solution is mixed.With filter paper solution is filtered, and obtain ethyl acetate extract, handle with chloroform afterwards and remove caffeine, concentrate then with the ethyl acetate processing.With the solution that obtains by the agarose gel post, and with the mixture drip washing of dichloromethane and methanol (1:1), then with leacheate at 40 ℃ of concentrated down catechin powder that obtain.
The separation of reference example 1 adipose cell (lipocyte)
To be cut into small pieces from the isolated epididymal adipose of male SD rat, and add 0.1 weight % collagenase (in the DMEM culture fluid, not adding phenol red), and after 2 hours, filter and obtain lipocyte (adipose cell) 37 ℃ of cultivations.
Then, in order to confirm that every kind of composition to the promotion ability that the neutral fat in the lipocyte of male SD rat decomposes, uses the above-mentioned lipocyte that obtains to experimentize.With 1 * 10
6Individual cells/well is containing 0.5% bovine serum albumin (BAS) and the Da Erbaikeshi improvement Yi Geershi culture medium of fatty acids (DMEM (Dulceco ' s modified eagles medium) or not in cultivated and use in each experiment 2 hours.
The differentiation of reference example 2 adipose cells (lipocyte)
With the fibroblast cell line 3T3-L1 cell of rat with 1 * 10
5Individual cells/well is inoculated in the culture plate in 6 holes, and is containing cultivation in the Da Erbaikeshi improvement Yi Geershi culture medium (DMEM, GIBCO BRL, Life Technologes) of 10% hyclone (FBS).Cultivate two days later, change culture medium into new DMEM (containing 10%FBS), cultivated again two days.Then cultured cell is placed on contain 1 mcg/ml insulin, 0.5 mM/liter IBMX and the new DMEM of 0.25 micromoles per liter dexamethasone (dexamethasone) in (containing 10 weight %FBS) induce differentiation, two days later, culture medium is changed into the new DMEM of insulin-containing and cultivating 5 days.Cultivate after 5 days, change culture medium into normal DMEM (containing 10%FBS) and observation, become adipose cell (lipocyte) up to cell.
Reference example 3 fat-splitting assay methods
By measuring the concentration that is separated to the glycerol the culture medium from adipose cell, the degree of decomposition of observation fat.Use is from Sigma (St.Louis, MO, U.S.A.) the GPO-trinder test kit of Gou Maiing, utilization color development reaction method quantitative assay glycerol, and the light absorption value when using enzyme immunoassay plate reading (ELISA reader) to measure 540 nanometers, then the data of contrast are made as 100% result who calculates various compositions.Contrast is to cultivate under the condition of not testing material or comparison material, on the other hand, theanine, caffeine, genistein, L-carnitine and catechin is added in the sample, and the concentration of each effective ingredient is 10 micromoles per liter.
EXPERIMENTAL EXAMPLE 1 theanine promotes fat-splitting effect
In order to measure the effect that theanine promotes the neutral fat decomposition of adipose cell, use 3T3-L1 adipose cell in reference example 2 differentiation.
With 3T3-L1 adipose cell washed twice, contain the 0.5 weight % bovine serum albumin (BAS) and the DMEM of fatty acids not toward wherein adding with phosphate buffered saline (PBS) (PBS) then.Buy theanine (greater than 97%) from KuridaKogyo (Japan), use is from Sigma (St.Louis, MO, U.S.A.) the GPO-trinder test kit of Gou Maiing, utilization color development reaction method quantitative assay glycerol, and the light absorption value when using enzyme immunoassay plate reading (ELISA reader) to measure 540 nanometers.Under the condition of not testing material or comparison material, cultivate contrast, then the data of contrast are made as 100% result who calculates various compositions.In addition, will be used as positive control, by measuring the concentration that is separated to the glycerol the culture medium from adipose cell, the degree of decomposition of observation fat by the sample that same concentration of caffeine was handled.The result as shown in Figure 1.
As can be seen from Figure 1, compared with the control, the sample of being handled by the theanine that extracts from green tea, the glycerol concentration that is separated to the culture medium from adipose cell has increased.In addition, theanine can not cause cytotoxicity when high concentration, and lipolytic effect is better than known positive comparative control caffeine with the effect of reducing fat.
The effect that EXPERIMENTAL EXAMPLE 2 theanine control β 3 adrenoceptors are expressed
In order to confirm that theanine promotes the mechanism that neutral fat decomposes, and uses the 3T3-L1 adipose cell of differentiation in reference example 2.Use 0.005% theanine (greater than 97%) and contrast material (caffeine, contrast) to handle cell, after 24 hours, from cell, extract RNA and carry out reverse transcriptional PCR (RT-PCR) available from KuridaKogyo (Japan).The result as shown in Figure 2.The RT-PCR test kit is available from TaKaRa, and the primer of β 3 adrenoceptors is available from Bioneer.
As can be seen from Figure 2, with the theanine processing expression of β 3 adrenoceptors has been increased, and the expression of β 3 adrenoceptors is fat-splitting signals in the adipose cell, confirms that therefore the lipolytic effect of theanine is because the increase of expression of receptor.
EXPERIMENTAL EXAMPLE 3 catechins suppress the effect of adipose cell differentiation
In order to measure the effect that catechin promotes that neutral fat decomposes in the adipose cell, use fibroblast cell line to cultivate and measure the experiment of absorption value.
The 3T3-L1 adipose cell of differentiation in the reference example 2 is washed three times with phosphate buffered saline (PBS) (PBS), and with extract buffer (20 mMs/liter Tris (Tris), 1 mM/liter ethylenediaminetetraacetic acid (EDTA) and 1 mM/liter 2 mercapto ethanol) gather in the crops.On the ice base with the cell of results by G26 pin six times, 4 ℃, 15000 centrifugal accelerations (Xg) centrifugal 3 minutes down, collect supernatant and obtain cell extract.
In order to measure the expression of glycerol-3-phosphate dehydrogenase (GPDH), to contain 0.1 mol triethanolamine, 2.5 mMs/rise EDTA, 0.1 mM/rise 2 mercapto ethanol, 125 micromoles per liter nicotinamide adenine dinucleotide (NADH, reduced form) and the GPDH of 100 micromoles per liter dehydrogenation acetone phosphate (DHAP) chemical examination buffer join in the above-mentioned cell extract, under 340 nanometers, measure the minimizing of absorption value then in 2 minutes.With every milligram dA/ minute was that unit represents variable quantity.Under the condition of not testing material or comparison material, cultivate contrast, then the data of contrast are made as 100% result who calculates various compositions.The result as shown in Figure 3.
As shown in Figure 3, compared with the control, in cell differentiation procedure, catechin added to and can significantly suppress differentiation in the 3T3-L1 cell.
EXPERIMENTAL EXAMPLE 4 theanine and catechin suppress the effect of neutral fat
Influence the effect that neutral fat generates in order to measure theanine and catechin, use fibroblast cell line to cultivate and measure the experiment of absorption value.
(U.S.A.) the GPO-trinder test kit of Gou Maiing carries out the color development reaction to the neutral fat in the cell extract identical with EXPERIMENTAL EXAMPLE 3 that obtains for St.Louis, MO, and the light absorption value when using ELISA reader to measure 540 nanometers from Sigma in use.The result as shown in Figure 4.
As can be seen from Figure 4, compared with the control, when using theanine and catechin to handle the 3T3-L1 cell in cell differentiation procedure, the neutral fat amount reduces, and when above-mentioned two kinds of compositions used together, the minimizing meeting of cumulative neutral fat was more obvious.
EXPERIMENTAL EXAMPLE 5 theanine, caffeine, genistein and the lipolytic cooperative effect of L-carnitine
For whether theanine, caffeine, genistein and the L-carnitine that confirms to have separately different mechanisms when reducing fat shows cooperative effect when reducing fat, with above-mentioned substance each and mix handling by the big resin fat of reference example 1 isolating SD cell.When individual processing, the concentration of theanine, caffeine, genistein and L-carnitine is 40 micromoles per liter, when handling together, considers concentration balance, and the concentration of every kind of composition is 10 micromoles per liter.The result as shown in Figure 5.
As can be seen from Figure 5, compare with individual processing, when using four kinds of compositions to handle together, amount of isolated glycerol significantly reduces.Consider the decomposition results of fat of EXPERIMENTAL EXAMPLE 1-4, the increase that obtains among Fig. 5 is not only because the amount of effective ingredient increases, but also is because the cooperative effect that the interaction between the mechanism of each composition causes.
EXPERIMENTAL EXAMPLE 6 theanine, caffeine, genistein, L-carnitine and catechin are to skin irritant mensuration
By observing edema and the erythema on the New Zealand white rabbit skin, measure the skin irritation that causes by theanine, caffeine, genistein, L-carnitine and catechin.
Excipient, 10% theanine, caffeine, genistein, L-carnitine, catechin or its mixture are administered on the skin of New Zealand white rabbit, and smear 24 day every day, uses altogether 8 times.After using,, calculate total skin irritation index by score value addition with edema and erythema.Measure skin irritation index according to table 1, the result is as shown in table 2.According to (P.I.I.) (Draize of DraizeShi present situation skin irritation index (Draize ' s skin Primary Irritation Index), J.H., Appraisal of the safety of chemical in foods, drugs and cosmetics) the calculating skin irritation index.
Table 1
Table 2
| Composition | Present situation skin irritation index (0-4) |
| Excipient | 0.6 |
| 10% theanine solution | 0.8 |
| 10% caffeine solution | 0.7 |
| 10% genistein solution | 0.8 |
| 10%L-carnitine solution | 0.7 |
| 10% catechin solution | 0.8 |
| 10% theanine, caffeine, genistein, L-carnitine solution | 0.9 |
| 10% theanine, caffeine, genistein, L-carnitine, catechin solution | 0.8 |
As can be seen from Table 2, compared with the control, theanine, caffeine, genistein, L-carnitine and catechin do not cause skin irritation.
Embodiment 1-8 and comparative example 1-2
According to following table 3 and 4, fat-reducing/anti-liparitosis washing liquid of preparation embodiment 1-8 and comparative example 1-2.
Table 3
Table 4
The effect of weight reducing of EXPERIMENTAL EXAMPLE 7 compositionss
In order to verify the effect of weight reducing of the compositions for preparing among embodiment 1-8 and the comparative example 1-2, select the adult female (10 every group) in 100 20-30 years, these women do not have metabolic problems and BMI (body-mass index, (body weight (kilogram)/height (rice))
2) be 23-25.By massage the washing liquid compositions for preparing among embodiment 1-8 and the comparative example 1-2 is administered to femoribus internus then, every day 2 times, used for 8 weeks.Before using washing liquid and after using for 8 weeks, measure subcutaneous fat thickness, the checking effect.
Use has hyperacoustic Ultrasound-EuB415US scanner and measures the thickness of subcutaneous fat (unit: millimeter), use t method of inspection (Student t test) deal with data as positive verification, relatively use the result of compositions front and back, analytic statistics significance (significance p＜0.05).The result as shown in Figure 6.
As shown in Figure 6, compare with the comparative example 2 that only contains theanine with the comparative example 1 that does not contain effective composition, the embodiment 1-8 that contains plurality of active ingredients shows the more significant effect that reduces subcutaneous fat thickness.The embodiment 1 that particularly contains whole 5 kinds of effective ingredient shows obvious effect.
The tenacity effect of EXPERIMENTAL EXAMPLE 8 compositionss
In order to verify the tenacity effect of the compositions for preparing among embodiment 1-8 and the comparative example 1-2, skin firmness.Observer 1-9 is skin firmness marking, relatively uses the score value of compositions front and back, with Wilcoxon check analysis statistical significance (significance α=0.05).The result as shown in Figure 7.
As can be seen from Figure 7, the embodiment 1 that contains effective ingredient has increased skin firmness.
As mentioned above, compositions of the present invention contains theanine, caffeine, genistein, L-carnitine and catechin, and have neutral fat in the cell that reduces fat, by the triglyceride in the lipocyte (adipose cell) being hydrolyzed into free fatty and glycerol promotes fat-splitting good action, and can suppress fat, reduce liparitosis, make the skin and the bodily form tough and tensile more smooth.Therefore, compositions of the present invention can be used to lose weight and the cosmetic composition of anti-liparitosis, promotes the development of cosmetics industry.
Although invention has been described with reference to embodiment, within the scope of the invention, those skilled in the art can make amendment to the present invention, and the invention of modification also obviously within the scope of the present invention.
Claims (6)
1. external use to lose weight compositions, said composition contain theanine and L-carnitine as effective ingredient, and perhaps said composition contains theanine, genistein and L-carnitine as effective ingredient, and said composition promotes the steatolysis in the adipose cell.
2. compositions according to claim 1, wherein, described effective ingredient also contains at least a material that is selected from the group of being made up of caffeine and catechin.
3. compositions according to claim 1, described theanine are to be selected from least a in the group of being made up of L-theanine, D-theanine and DL-theanine.
4. compositions according to claim 2, described catechin is for being selected from least a in the group of being made up of (+) catechin, (-) epicatechin, (-) epicatechin-3-gallate, (+) epicatechin and (-) epicatechin gallate.
5. compositions according to claim 1, described theanine is promoted the expression of β 3 adrenoceptors, promotes steatolysis.
6. compositions according to claim 1 and 2, described content of effective are the 0.0001-20 weight % of total composition.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020030026015 | 2003-04-24 | ||
| KR10-2003-0026015 | 2003-04-24 | ||
| KR10-2003-0026015A KR100513616B1 (en) | 2003-04-24 | 2003-04-24 | Composition for external application to the skin containing the active ingredients of green tea |
| KR1020030098859 | 2003-12-29 | ||
| KR10-2003-0098859 | 2003-12-29 | ||
| KR1020030098859A KR100998990B1 (en) | 2003-12-29 | 2003-12-29 | Composition for slimming |
| PCT/KR2004/000947 WO2004093865A1 (en) | 2003-04-24 | 2004-04-23 | Composition for slimming |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910209491XA Division CN101690722B (en) | 2003-04-24 | 2004-04-23 | Composition for slimming |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1777418A CN1777418A (en) | 2006-05-24 |
| CN1777418B true CN1777418B (en) | 2010-09-08 |
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| CN2004800107268A Expired - Fee Related CN1777418B (en) | 2003-04-24 | 2004-04-23 | Composition for slimming |
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| KR (1) | KR100513616B1 (en) |
| CN (1) | CN1777418B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100896500B1 (en) * | 2006-12-28 | 2009-05-08 | (주)코바스 | Transdermal composition for removing cellulite, cataplasma comprising the same and method for preparing thereof |
| KR101700418B1 (en) * | 2010-07-02 | 2017-01-31 | (주)아모레퍼시픽 | Composition of skin external application for slimming containing Broussonetia kazinoki extracts |
| KR101956155B1 (en) * | 2011-11-23 | 2019-06-25 | (주)아모레퍼시픽 | Skin external composition comprising theanine derivative |
| WO2013077574A1 (en) * | 2011-11-23 | 2013-05-30 | 주식회사 아모레퍼시픽 | Topical composition containing theanine derivatives for use on skin |
| KR101656682B1 (en) * | 2015-06-01 | 2016-09-12 | (주)아모레퍼시픽 | Composition for improving diabetes mellitus and for promoting the differentiation of human mesenchymal stem cell to adipocytic cells containing (-)catechin, (-)epicatechin gallate, (-)gallocatechin |
| CN107029237B (en) * | 2016-02-04 | 2021-06-25 | 康建胜 | Application of thermogenesis enhancing compound in enhancing thermogenesis of noradrenaline compound induced brown fat cells |
| CN109674696A (en) * | 2018-03-27 | 2019-04-26 | 上海同柏生物科技有限公司 | One kind is for stomach fat degradation technique and its preparation and application |
| WO2021056248A1 (en) * | 2019-09-25 | 2021-04-01 | 上海同柏生物科技有限公司 | Technology for facial fat degradation and firming and application of technology |
| FR3126870A1 (en) * | 2021-09-16 | 2023-03-17 | Gozen K. K. Japan | Pharmaceutical or cosmetic composition intended to improve cellulite and varicose veins, characterized in that it comprises a combination of theanine and polysaccharides derived from matcha. |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002265359A (en) * | 2001-03-07 | 2002-09-18 | Ito En Ltd | Kainic acid type glutamate receptor-shielding agent, ampa type glutamate receptor shielding agent, brain- protecting agent and food or drink having brain- protecting function |
| US6462051B1 (en) * | 2001-04-25 | 2002-10-08 | Ito En, Ltd. | Composition for reducing mental fatigue, composition for maintaining and enhancing concentration, and composition for maintaining and enhancing mental vigor |
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2003
- 2003-04-24 KR KR10-2003-0026015A patent/KR100513616B1/en active IP Right Grant
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002265359A (en) * | 2001-03-07 | 2002-09-18 | Ito En Ltd | Kainic acid type glutamate receptor-shielding agent, ampa type glutamate receptor shielding agent, brain- protecting agent and food or drink having brain- protecting function |
| US6462051B1 (en) * | 2001-04-25 | 2002-10-08 | Ito En, Ltd. | Composition for reducing mental fatigue, composition for maintaining and enhancing concentration, and composition for maintaining and enhancing mental vigor |
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| Title |
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| ZHANG G. ET AL.Effects of dietary powdered green tea and theanine on tumorgrowth and endogenoushyperlipidemia in hepatoma-bearing rats..BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY66 4.2002,66(4),711-716. * |
| ZHANG G. ET AL.Effects of dietary powdered green tea and theanine on tumorgrowth and endogenoushyperlipidemia in hepatoma-bearing rats。.BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY66 4.2002,66(4),711 - 716. * |
| 吕毅等.茶氨酸的生理作用及合成.茶 叶 科 学23 1.2003,23(1),1-5. * |
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| 杨焱等.茶叶中儿茶素的提取和纯化工艺研究综述.茶业通报19 4.1997,19(4),34-36. * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100513616B1 (en) | 2005-09-09 |
| KR20040092538A (en) | 2004-11-04 |
| CN1777418A (en) | 2006-05-24 |
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