CN1775054A - Method extracting fluorine from tea for determining fluorine content of tea - Google Patents
Method extracting fluorine from tea for determining fluorine content of tea Download PDFInfo
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- CN1775054A CN1775054A CN 200510122654 CN200510122654A CN1775054A CN 1775054 A CN1775054 A CN 1775054A CN 200510122654 CN200510122654 CN 200510122654 CN 200510122654 A CN200510122654 A CN 200510122654A CN 1775054 A CN1775054 A CN 1775054A
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Abstract
The present invention relates to a method for extracting fluorine from tea so as to measure fluorine content in the tea, also can be used for extracting some component from other plant. Said method includes the following steps: firstly, drying tea leaf, soaking whole tea leaf or crushed tea leaf in deionized water with 100 deg.C, then promptly placing said tea leaf into ultrasonic device wate bath with 40-80 deg.C and processing for 5-10 min, so that the extraction of fluorine content in the tea leaf is implemented, then using ion-selective electrode analysis process to measure its fluorine content.
Description
Technical field
The present invention relates to a kind of extracting method that is used for the tealeaves fluorine content measurement, also can be used for the extraction of other some composition of plant.
Background technology
Tea tree has the function of fluorine in the enrichment environment.Tea is one of the most popular beverage, and drinking tea also is one of main source of human body absorption fluorine.It is useful that human body is suitably taken in fluorine, but excessive absorption fluorine is harmful to health, can cause fluorine poisoning, causes tooth enamel spot and pigementation, can cause loss of tooth when serious, causes fluorosis of bone.
At present the preprocess method that adopts Fluoride ion selective electrode method to measure water dissolvable fluorine content in the tealeaves mainly contains: pulverize boiling water bath method, full leaf infusion method, hydrochloric acid infusion method and alkaline leaching etc., but exist that pretreatment time is long, recovery rate is low, need with problems such as chemicals.Huang Wenyao, Li Yang, Li Mingjian, Deng. three kinds of distinct methods are measured the comparison of fluorine content in the brick tea. the sick magazine of learning of place of china, 2002,21 (1): the different extracting method fluorine content measurement value of 61-62. report is: pulverize boiling water bath method＞full leaf infusion method＞hydrochloric acid infusion method, full leaf infusion method is than pulverizing boiling water bath method low about 30%; Ma Lifeng, Shi Yuan value, Ruan Jianyun etc. China's tealeaves fluorine content status research [J]. agriculture environmental protection .2002,21 (6): 537-539. reports that the fluorine content that full leaf infusion method is wanted to extract after 45 minutes just tends towards stability.
Summary of the invention
The objective of the invention is to propose a kind of extracting method that is used for the tealeaves fluorine content measurement, the fluorine content measurement that heatable ultrasonic unit is used for tealeaves is extracted, and makes it to have tangible reinforcement extraction effect.
Realize the technical scheme of above-mentioned purpose:
At first with tea-drying, with the tealeaves that 100 ℃ deionized waters brew the full leaf of tea or pulverize, to put into immediately then and be heated to 40-80 ℃, the supersonic oscillations frequency is 40KHz, handled in the ultrasonic unit water-bath 5～10 minutes, and promptly finished the extraction of tealeaves fluorine content.Just can carry out later measurement operation step afterwards by the method that ion selective electrode method is measured fluorine content in the tealeaves.
The processing time is 5 minutes in the preferable water-bath.
Beneficial effect of the present invention is as follows:
1. the present invention has tangible reinforcement extraction effect, can shorten extraction time, only needs about 5 minutes, and extraction effect is equivalent to pulverize the boiling water bath method, greater than full leaf infusion method and hydrochloric acid infusion method.
2. when the present invention is used for the extraction of tealeaves fluorine content measurement, the granule size of tealeaves does not have remarkable influence to the extraction of fluorine ion, the full leaf of tea is more approaching with the detected fluorine content of tea dust that pulverizes, crosses 40 mesh sieves, both differ about 5%, can save the time-consuming process of effort again that tealeaves is pulverized in less demanding occasion.
The specific embodiment
The following examples will be further detailed the present invention, but therefore not limit the present invention.
The ultrasonic unit maximum heating temperature that heating used herein is adjustable is that 80 ℃, supersonic oscillations frequency are 40KHz, and exporting ultrasonic electrical power is 100W.
Embodiment 1
Take by weighing 1.01g in 80 ℃ of baking ovens dry 40 minutes and pulverize, cross the tealeaves of 40 mesh sieves, place the conical flask of 250mL drying, add 200mL, 100 ℃ deionized water, in 60 ℃ ultrasound bath, handled 5 minutes then, be cooled to room temperature with running water again, be transferred in the 250mL volumetric flask without filtration, be settled to scale, mixing.Treat the tealeaves post precipitation, draw the 10mL supernatant and place the 50mL volumetric flask, add 25mL total ionic strength adjustment buffer degree regulator solution, be diluted to scale with deionized water, mixing.The tealeaves fluorine content that records is 184.5mg/kg.
Embodiment 2
Take by weighing 1.00g in 80 ℃ of baking ovens dry 40 minutes without the tealeaves that pulverizes (with embodiment 1 with a kind of), place the conical flask of 250mL drying, add 200mL, 100 ℃ deionized water, in 80 ℃ ultrasound bath, handled 5 minutes then, be cooled to room temperature with running water again, be transferred in the 250mL volumetric flask without filtration, be settled to scale, mixing.Treat the tealeaves post precipitation, draw the 10mL supernatant and place the 50mL volumetric flask, add 25mL total ionic strength adjustment buffer degree regulator solution, be diluted to scale with deionized water, mixing.The tealeaves fluorine content that records is 178.0mg/kg.
Embodiment 3
Take by weighing 1.01g in 80 ℃ of baking ovens dry 40 minutes and pulverize, cross 40 mesh sieves tealeaves (with embodiment 1 with a kind of), place the conical flask of 250mL drying, add 200mL, 100 ℃ deionized water, in 40 ℃ ultrasound bath, handled 5 minutes then, be cooled to room temperature with running water again, be transferred in the 250mL volumetric flask without filtration, be settled to scale, mixing.Treat the tealeaves post precipitation, draw the 10mL supernatant and place the 50mL volumetric flask, add 25mL total ionic strength adjustment buffer degree regulator solution, be diluted to scale with deionized water, mixing.The tealeaves fluorine content that records is 182.3mg/kg.
Relation between different excusing from death ripple times and the fluorine content:
Take the different excusing from death ripple time to carry out the extraction of fluorine content respectively embodiment 1 described tealeaves, the result is as follows:
| Time/min | 5 | 10 | 15 | 20 | 30 | 40 | 50 |
| Fluorine content/mg/kg | 184.5 | 184.0 | 183.6 | 185.2 | 185.9 | 184.7 | 186.1 |
Claims (2)
1. the extracting method of fluorine in the tealeaves that is used for measuring tealeaves fluorine content, it is characterized in that at first with tea-drying, the tealeaves that 100 ℃ deionized waters are brewed the full leaf of tea or pulverize, put into the ultrasonic unit water-bath that is heated to 40-80 ℃ then immediately and handled 5～10 minutes, promptly finished the extraction of tealeaves fluorine content.
2. the extracting method of fluorine is characterized in that the processing time is 5 minutes in the preferable water-bath in a kind of tealeaves that is used for measuring tealeaves fluorine content according to claim 1.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101226542A CN100353852C (en) | 2005-11-30 | 2005-11-30 | Method extracting fluorine from tea for determining fluorine content of tea |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005101226542A CN100353852C (en) | 2005-11-30 | 2005-11-30 | Method extracting fluorine from tea for determining fluorine content of tea |
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| Publication Number | Publication Date |
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| CN1775054A true CN1775054A (en) | 2006-05-24 |
| CN100353852C CN100353852C (en) | 2007-12-12 |
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| CNB2005101226542A Expired - Fee Related CN100353852C (en) | 2005-11-30 | 2005-11-30 | Method extracting fluorine from tea for determining fluorine content of tea |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366424B (en) * | 2008-09-16 | 2011-01-05 | 无锡太阳绿宝科技有限公司 | Preparation technique for high-fluorine tea extract |
| CN101334379B (en) * | 2007-06-29 | 2012-07-25 | 上海宝钢工业检测公司 | Water fluorine content determination method |
| CN103383320A (en) * | 2013-07-11 | 2013-11-06 | 哈尔滨医科大学 | Brick tea fluorine component analysis quality control samples and preparation method thereof |
| CN104713909A (en) * | 2015-04-10 | 2015-06-17 | 湖南农业大学 | Simple method for authenticating fluorine injury of plants |
| CN104970147A (en) * | 2015-04-17 | 2015-10-14 | 中华全国供销合作总社杭州茶叶研究所 | Preparation method of high-fluorine high-chlorophyll tea leaf extract |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1025105C (en) * | 1989-01-10 | 1994-06-22 | 席国华 | Method of making gum against carious tooth |
-
2005
- 2005-11-30 CN CNB2005101226542A patent/CN100353852C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101334379B (en) * | 2007-06-29 | 2012-07-25 | 上海宝钢工业检测公司 | Water fluorine content determination method |
| CN101366424B (en) * | 2008-09-16 | 2011-01-05 | 无锡太阳绿宝科技有限公司 | Preparation technique for high-fluorine tea extract |
| CN103383320A (en) * | 2013-07-11 | 2013-11-06 | 哈尔滨医科大学 | Brick tea fluorine component analysis quality control samples and preparation method thereof |
| CN104713909A (en) * | 2015-04-10 | 2015-06-17 | 湖南农业大学 | Simple method for authenticating fluorine injury of plants |
| CN104970147A (en) * | 2015-04-17 | 2015-10-14 | 中华全国供销合作总社杭州茶叶研究所 | Preparation method of high-fluorine high-chlorophyll tea leaf extract |
| CN104970147B (en) * | 2015-04-17 | 2018-05-04 | 中华全国供销合作总社杭州茶叶研究所 | A kind of preparation method of high fluorine high chlorophyll tea extract |
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| Publication number | Publication date |
|---|---|
| CN100353852C (en) | 2007-12-12 |
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Granted publication date: 20071212 Termination date: 20101130 |