CN104713909A - Simple method for authenticating fluorine injury of plants - Google Patents
Simple method for authenticating fluorine injury of plants Download PDFInfo
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- CN104713909A CN104713909A CN201510168620.0A CN201510168620A CN104713909A CN 104713909 A CN104713909 A CN 104713909A CN 201510168620 A CN201510168620 A CN 201510168620A CN 104713909 A CN104713909 A CN 104713909A
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- fluorine
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- oil repellent
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Abstract
The invention relates to a simple method for authenticating fluorine injury of plants. The method comprises the steps of detecting fluorine contents of injured plant leaves and same normal plant leaves by virtue of a fluorine ion selective electrode method, and judging whether the plant injury is caused by fluoride by virtue of comparison of fluorine contents of the leaves. The simple method can be utilized for simply, rapidly and accurately judging whether the plants are injured by fluoride and authenticating the fluorine injury at the initial stage, so that the fluorine injury can be timely controlled, and the irreversible fluorine injury of the plants is prevented.
Description
Technical field
The present invention relates to the method for plant identification fluorine injury, be specifically related to a kind of passing through and detect plant leaf blade Oil repellent and judge the method whether plant injures by fluoride accurately, fast.
Background technology
In recent years, the case of fluoride harm plant happens occasionally.In the qualification to plant fluorine evil; the typical injury symptom that usual meeting shows according to plant judges; but due to the impact of fluorinated species and floristic difference and environmental factor; the symptom that plant shows after being subject to fluoride injury is also not quite identical; even kindred plant is also variant by the symptom showed after fluoride injury under various circumstances, and the injury symptom therefore only according to plant performance can not determine whether plant injures by fluoride.Moreover when judging that plant is subject to fluoride injury by typical fluorine injury symptom, fluorine evil often enters the middle and later periods, now take corresponding control measures late for it again.Existing large quantity research shows, plant leaf blade is the main accumulation place of fluoride, and plant just there will be injury symptom when fluoride is accumulated to a certain amount of in plant leaf blade, therefore can judge whether plant injures by fluoride by detecting plant leaf blade Oil repellent.
Fluoride ion selective electrode method is the common method that food service industry is used for detecting Fluorine in Foods ion, but there are no the method being used for the report of detection of the Oil repellent of plant own.
Summary of the invention
Technical matters to be solved by this invention is: for the deficiencies in the prior art, provides the simple and easy method that a kind of plant identification fluorine injures, and judges whether plant is subject to fluoride injury quickly and accurately by detecting the fluorine of plant leaf blade.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:, a kind of plant identification fluorine injury simple and easy method, be characterized in, the method is the Oil repellent adopting Fluoride ion selective electrode method to detect impaired plant leaf blade and normal kindred plant blade, judges whether plant is subject to fluorine damage by contrast blade Oil repellent.
Particularly, the method plucks plant leaf blade to be measured, clean, air-dry, in 75-85 DEG C of dry 1.5-2.5h, pulverized 40 mesh sieves; In 1g blade powder, add the hydrochloric acid sealing lixiviate 1-1.5h of 10mL 3% (V/V), constantly shake gently during lixiviate; The total ionic strength damping fluid 25mL that the sodium acetate solution adding isopyknic 3.0mol/L volumetric molar concentration again mixes with the sodium citrate solution of 0.75mol/L volumetric molar concentration, add water and be settled to 50mL, shake up, its potential value is measured with fluorine ion detector, potential value is substituted into fluoride typical curve and try to achieve Oil repellent, and the Oil repellent of this Oil repellent with the normal kindred plant blade of trying to achieve according to said method is compared.
Compared with prior art, advantage of the present invention is: the Fluoride ion selective electrode method of food service industry be applied in the detection of the Oil repellent of plant own, judges whether plant injury is caused by fluoride by the detection to impaired plant leaf blade and normal kindred plant blade Oil repellent, comparison.Can judge whether plant is subject to fluorine injury simply, accurately and rapidly, and just can identify at the fluorine injury initial stage, fluorine evil be controlled in time, prevents plant from damaged by irreversible fluorine.
Embodiment
The simple and easy method of plant identification fluorine injury of the present invention plucks plant leaf blade to be measured, clean, air-dry, is placed in air dry oven at 75-85 DEG C of dry 1.5-2.5h, then pulverized 40 mesh sieves; With the above-mentioned blade powder of 1g in 50mL volumetric flask, add the hydrochloric acid sealing lixiviate 1-1.5h of 10mL 3% (V/V), during lixiviate, constantly shake volumetric flask gently; Add 25mL total ionic strength damping fluid (sodium acetate solution of isopyknic 3.0mol/L volumetric molar concentration mixes with the sodium citrate solution of 0.75mol/L volumetric molar concentration) again, add water and be settled to 50mL, shake up, its potential value is measured with fluorine ion detector, potential value is substituted into fluoride typical curve and try to achieve Oil repellent, and the Oil repellent of this Oil repellent with the normal kindred plant blade of trying to achieve according to the method is compared, the Oil repellent of impaired plant leaf blade is higher than the Oil repellent of normal kindred plant blade.
Embodiment 1
After use for laboratory 10mmol/L potassium fluoride solution cultivates plant seedlings (peanut, radish, corn, paddy rice, paddy rice two leaf stage seedling) 48h, all there is injury symptom in various degree in all plant seedlings, illustrates that fluoride result in plant injury.Show impaired plant leaf blade Oil repellent testing result, all impaired plant leaf blade Oil repellent, all higher than normal value, see the following form 1, illustrate whether plant leaf blade Oil repellent can be subject to the judge index of fluorine damage as plant.
Plant leaf blade Oil repellent after table 1 10mmol/L potassium fluoride solution cultivation 48h
| Peanut seedling | Radish seedling | Corn seedling | Rice seedling | Paddy rice two leaf stage seedling | |
| Normal blade Oil repellent (mg/kg) | 1.50 | 0.50 | 1.00 | 2.49 | 1.99 |
| Damaged blade Oil repellent (mg/kg) | 17.98 | 21.04 | 27.98 | 52.80 | 47.94 |
Embodiment 2
Build new hollow brick field more than 3000, adjacent nectarine garden fruit tree with Yueyang to lose and receive, to the testing result of nectarine blade Oil repellent be: damaged blade Oil repellent is 37.30mg/kg, contrasting normal nectarine blade Oil repellent is 11.39mg/kg, judges that the fluorine-containing coal smoke that this brick field discharges causes nectarine to lose the main cause received.
Embodiment 3
Near the fluorine chemistry factory of Chenzhou, Activities of Some Plants (sweet osmanthus, podocarpus, corn, metasequoia and photinia glabra) is impaired, impaired plant leaf blade Oil repellent testing result is shown, all impaired plant leaf blade Oil repellent are all higher than normal value, see the following form 2, judge that the fluorine-containing discarded object that fluorine chemistry factory discharges is the main cause causing neighbouring plant impaired.
Plant leaf blade Oil repellent testing result near the fluorine chemistry factory of table 2 Chenzhou
| Sweet osmanthus | Podocarpus | Corn | Metasequoia | Photinia glabra | |
| Normal blade Oil repellent (mg/kg) | 127.41 | 103.09 | 46.09 | 494.26 | 122.13 |
| Damaged blade Oil repellent (mg/kg) | 164.28 | 122.13 | 494.26 | 857.31 | 352.18 |
Claims (2)
1. a simple and easy method for plant identification fluorine injury, is characterized in that, the method is the Oil repellent adopting Fluoride ion selective electrode method to detect impaired plant leaf blade and normal kindred plant blade, judges whether plant is subject to fluorine damage by contrast blade Oil repellent.
2. the simple and easy method of a kind of plant identification fluorine injury as claimed in claim 1, it is characterized in that, the pre-treatment of the method, is pluck plant leaf blade to be measured, clean, air-dry, in 75-85 DEG C of dry 1.5-2.5h, pulverizes 40 mesh sieves; In 1g blade powder, add the hydrochloric acid sealing lixiviate 1-1.5h of 10mL 3% volumetric concentration, constantly shake gently during lixiviate; The total ionic strength damping fluid 25mL that the sodium acetate solution adding isopyknic 3.0mol/L volumetric molar concentration again mixes with the sodium citrate solution of 0.75mol/L volumetric molar concentration, adds water and is settled to 50mL, shake up, be used further to fluorine ion detector and measure its potential value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510168620.0A CN104713909A (en) | 2015-04-10 | 2015-04-10 | Simple method for authenticating fluorine injury of plants |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510168620.0A CN104713909A (en) | 2015-04-10 | 2015-04-10 | Simple method for authenticating fluorine injury of plants |
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| CN104713909A true CN104713909A (en) | 2015-06-17 |
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| CN201510168620.0A Pending CN104713909A (en) | 2015-04-10 | 2015-04-10 | Simple method for authenticating fluorine injury of plants |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2432156A1 (en) * | 2000-12-19 | 2002-06-27 | Nicast Ltd. | Method and apparatus for manufacturing polymer fiber shells via electrospinning |
| CN1775054A (en) * | 2005-11-30 | 2006-05-24 | 江苏工业学院 | Method extracting fluorine from tea for determining fluorine content of tea |
| CN102175745A (en) * | 2011-03-14 | 2011-09-07 | 天津师范大学 | Method for quickly detecting fluorine content in specific vegetables |
| US20120149569A1 (en) * | 2005-03-04 | 2012-06-14 | Monsanto Technology Llc | Mitigating Necrosis in Transgenic Glyphosate-Tolerant Cotton Plants Treated with Herbicidal Glyphosate Formulations |
| CN103383320A (en) * | 2013-07-11 | 2013-11-06 | 哈尔滨医科大学 | Brick tea fluorine component analysis quality control samples and preparation method thereof |
| CN103424443A (en) * | 2013-05-28 | 2013-12-04 | 中国水稻研究所 | Ion-selective electrode method for measuring fluoride content in grains, vegetables and fruits |
-
2015
- 2015-04-10 CN CN201510168620.0A patent/CN104713909A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2432156A1 (en) * | 2000-12-19 | 2002-06-27 | Nicast Ltd. | Method and apparatus for manufacturing polymer fiber shells via electrospinning |
| US20120149569A1 (en) * | 2005-03-04 | 2012-06-14 | Monsanto Technology Llc | Mitigating Necrosis in Transgenic Glyphosate-Tolerant Cotton Plants Treated with Herbicidal Glyphosate Formulations |
| CN1775054A (en) * | 2005-11-30 | 2006-05-24 | 江苏工业学院 | Method extracting fluorine from tea for determining fluorine content of tea |
| CN102175745A (en) * | 2011-03-14 | 2011-09-07 | 天津师范大学 | Method for quickly detecting fluorine content in specific vegetables |
| CN103424443A (en) * | 2013-05-28 | 2013-12-04 | 中国水稻研究所 | Ion-selective electrode method for measuring fluoride content in grains, vegetables and fruits |
| CN103383320A (en) * | 2013-07-11 | 2013-11-06 | 哈尔滨医科大学 | Brick tea fluorine component analysis quality control samples and preparation method thereof |
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Application publication date: 20150617 |