CN1772403A - Method of utilizing rumen microbe in degrading waste plant matter effectively - Google Patents
Method of utilizing rumen microbe in degrading waste plant matter effectively Download PDFInfo
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- CN1772403A CN1772403A CN 200410065682 CN200410065682A CN1772403A CN 1772403 A CN1772403 A CN 1772403A CN 200410065682 CN200410065682 CN 200410065682 CN 200410065682 A CN200410065682 A CN 200410065682A CN 1772403 A CN1772403 A CN 1772403A
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- fermentation tank
- buffer solution
- bed material
- anaerobic digestion
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Abstract
The present invention relates to the biotechnology of treating plant waste and utilizing the relevant resource. The method of utilizing rumen microbe in degrading plant waste includes the following steps: 1. crushing or pulping the plant waste into base material; 2. taking out rumen microbe from rumen of ruminant, culturing and domesticating in a fermenting tank to eliminate methane bacteria, and inoculating to anaerobic digestion reactor with the base material and mineral buffering liquid; 3. running the anaerobic digestion reactor to hydrolyze and acidifying the plant waste; and 4. collecting gaseous product, liquid phase product and solid phase product. The degradation process of the present invention releases hydrogen ions in hydrogen gas form and produces no methane, being favorable to environment protection. The liquid phase product is short chain organic acid as hydrogen producing material, and the solid phase product has rich protein and may be treated into organic fertilizer.
Description
Technical field:
The present invention relates to the biotechnology of botanical waste processing and recycling thereof.
Background technology:
The a large amount of botanical waste of the annual generation of China, for example the annual production of various agricultural crop straws is just more than 600,000,000 tons in the rural area, and existing treating method mostly is to burn on the spot, and its result usually causes boundless smog, severe contamination environment, also wasted preciousness and Limited resources.In the city, people's house refuse is the primary pollution source in city equally, also exists a large amount of botanical wastes in the house refuse.From the angle of protection environment, how handling and utilizing these botanical wastes is current very popular topic.
Existing treatment technology comprises two kinds of chemical method and biological technique methods.Wherein biological technique method is owing to environmental friendliness, with low cost coming into one's own.2003 the 48th the 4th phases of volume of Britain magazine " hydroscience and technology " (Water Science and Technology) have been reported the method for utilizing rumen microorganism anaerobic degradation straw refuse.This method is earlier with crushed stalk, intermittent then joining makes the botanical waste degraded in the anaerobic digestion reaction vessel of inoculating rumen microorganism, its primary product is acetate, propionic acid, butyric acid and methane, and the burning of methane and discharging still can cause serious greenhouse effects.
Summary of the invention:
The objective of the invention is to, provide a kind of and utilize rumen microorganism to come degrading plant matter discarded object efficiently, and make the method that does not contain methane in the catabolite through domestication.
Purpose of the present invention realizes in the following manner.
The method of utilizing rumen microorganism to come degrading plant matter discarded object of the present invention, comprise that (1) is broken or pull an oar into standby bed material with botanical waste, (2) in ruminant tumor gastric, take out rumen microorganism, and be inoculated in the anaerobic digestion reaction vessel that fills botanical waste bed material and mineral matter buffer solution, (3) start the operation anaerobic digestion reaction vessel, make the phyteral hydrolysis acidification, (4) collect gas, liquid product and the solid product that produces.It is characterized in that, describedly in ruminant tumor gastric, take out rumen microorganism, at first must be inoculated into and cultivate in the fermentation tank and tame, its inoculum concentration is the 5-30% of fermentation tank reaction volume, fill botanical waste bed material and mineral matter buffer solution in this fermentation tank, the concentration of phyteral bed material is 10-40g/L.The domestication condition is: temperature is 30-43 ℃ in the fermentation tank, stir speed (S.S.) 50-100 rev/min, the interpolation speed of botanical waste bed material is 20-40g/L/d, the time of staying of mineral matter buffer solution in fermentation tank is 12-24 hour, keep that pH is 6.0-7.0 in the fermentation tank, the time of staying 48-72 of botanical waste bed material in fermentation tank hour by regulating in the buffer solution concentration sodium hydroxide.(domestication of expression rumen microorganism is finished) inoculates in the anaerobic digestion reaction vessel that fills botanical waste bed material and mineral matter buffer solution and enters the routine operation program when detecting less than methane up to only detect hydrogen and carbon dioxide continuous two weeks in fermentation tank institute aerogenesis body composition.
Mineral matter buffer solution described in the present invention is that wear fragrant buffer solution, agate examined mineral matter buffer solutions such as buffer solution, the fragrant buffer solution of wearing consist of NaHCO
35g/L, Na
2HPO
41.9g/L, KCl 0.5g/L, NaCl 0.5g/L, MgCl
2.6H
2O 0.13g/L, CaCl
20.05g/L, urea 0.5g/L; Agate is examined the Na that consists of of buffer solution
2CO
33.1g/L, K
2CO
34.0g/L, Na
2HPO
40.9g/L, K
2HPO
41.1g/L, MgCl
2.6H
2O 0.10g/L, CaCl
20.04g/L, urea 0.5g/L.
The present invention utilizes the rumen microorganism of domestication, with agricultural solid residue, water plant and phyteral organic waste efficient degradation in anaerobic digestion reaction vessel, degradation efficiency reaches 55-75%, and the gas of generation is hydrogen and carbon dioxide, can obtain pure hydrogen after simple purification is handled; The liquid product that produces is mainly short chain volatile organic acids such as acetate, propionic acid, butyric acid, and these organic acids can be as the raw material of further producing hydrogen; The solid product that produces can become colory fertilizer owing to be rich in albumen after compost treatment.Can find out that thus the present invention has produced the effect that turns waste into wealth, manufacturing cost is very cheap.
The rumen microorganism of using among the present invention is to extract from the ruminant body, it is the very capable mixed microorganism flora of occurring in nature decomposition of cellulose, agricultural crop straw, pasture and water and domestic organic garbage all had very strong capacity of decomposition, therefore, handle with respect to the anaerobic activated sludge method, degradation efficiency of the present invention has improved 30-50%.But, if directly being inoculated into anaerobic digestion reaction vessel, it uses, will contain a large amount of methane in the gas that degraded produces, and the burning of methane and release will produce serious greenhouse effects, destroy ecological environment.At first rumen microorganism has been carried out preliminary treatment among the present invention, promptly cultivate and tame processing, high concentration bed material and higher hydrolysis acidification speed have suppressed the growth and the breeding of methane backeria, add breeding cycle generation that methane backeria itself is long, make the interior methane backeria of reactor be cushioned the liquid wash-out gradually and come out methane backeria complete obiteration in final reactor.Therefore, the hydrogen ion that discharges in the degradation process finally discharges with the form of hydrogen, has avoided producing methane in the anaerobic digestion process, thereby has helped protecting environment more.
The specific embodiment
Below by embodiment technical scheme of the present invention is further described.
Embodiment 1:
1, the stalk behind the collection reaping corn, airing was the particle of diameter less than 5mm with pulverizer with crushed stalk after 5 days.
2, getting the 300ml rumen fluid from the fistula goat transfers to rapidly in 5 liters of fermentation tanks, add corn stalk pellets 80g and 3.7L mineral matter buffer solution, keep by regulating in the buffer solution concentration sodium hydroxide that pH is 6.0-6.4 in the fermentation tank, 32 ℃ of temperature, stir speed (S.S.) are 60 rev/mins.The interpolation speed of powder of straw remained 30g/L/d, and the time of staying of buffer solution in reactor dropped to the 6th day 18 hours gradually and remain unchanged from 24 hours, and the time of staying of stalk particle in reactor is 60 hours.Detect the composition that produces composition in the gas every day, and when detecting less than methane when having only hydrogen and carbon dioxide in continuous two all gas componants, methanogen is removed fully, and the rumen microorganism domestication is finished.
3, the rumen microorganism after domestication is finished is inoculated in the anaerobic digestion reaction vessel, keeps 1.2L/ branch clock rate to charge into nitrogen 20 minutes, drives away residual oxygen in the reactor, guarantees the anaerobic state in the reactor.Overall reactor is amassed 4.9L, and wherein the reaction zone volume is 2.5 liters.Corn stalk pellets is 23g/L with the concentration of buffer solution dilution, and the temperature of control reactor is 32 ℃, keeps by regulating in the buffer solution concentration sodium hydroxide that pH is 6.0-6.4 in the fermentation tank, and 18 hours buffer solution time of staying, stir speed (S.S.) is 60 rev/mins.The interpolation speed of bed material is increased to 30g/L/d after 7 days gradually from 10g/L/d.Residue after the degraded is discharged through outlet, is increased to 1000mL/d (being equivalent to 60 hours substrate time of staying) after 7 days gradually from the 250mL/d of beginning.When reaching balance, the degradation efficiency of corn stalk pellets reaches 72%, and the production efficiency of short chain organic acid reaches the 0.42g/g maize straw.Acetate 0.17g/g maize straw wherein, propionic acid 0.11g/g maize straw, butyric acid 0.10g/g maize straw, remaining is ethanol, isobutyric acid, valeric acid and isovaleric acid.Hydrogen content reaches 32% in the gas componant, and hydrogen output is the 70ml/g maize straw.
Embodiment 2:
1, collects family domestic garbage, reject the inorganic matter that wherein comprises, as stone, iron filings etc.It is less than the particle below the 6mm that cool solarization was pulverized after 5 days.
2, getting the 700ml rumen fluid from the fistula goat transfers to rapidly in 5 liters of fermentation tanks, add comminuted plants matter organic waste 80g and 3.3L mineral matter buffer solution, keep by regulating in the buffer solution concentration sodium hydroxide that pH is 6.3-6.7 in the fermentation tank, 37 ℃ of temperature, stir speed (S.S.) are 90 rev/mins.The interpolation speed of comminuted plants matter organic waste remains 40g/L/d, the time of staying of buffer solution in reactor dropped to the 6th day 12 hours gradually and remains unchanged from 24 hours, the time of staying of comminuted plants matter organic waste in reactor is 60 hours.Detect the composition that produces composition in the gas every day, and when detecting less than methane when having only hydrogen and carbon dioxide in continuous two all gas componants, methanogen is removed fully, and the rumen microorganism domestication is finished.
3, the rumen microorganism after domestication is finished is inoculated in the anaerobic digestion reaction vessel, and the speed that kept 2.0L/ minute charged into argon gas 15 minutes, drives away residual oxygen in the reactor, guarantees the anaerobic state in the reactor.Overall reactor is amassed 4.9L, and wherein the reaction zone volume is 2.5 liters.The phyteral organic waste is 15g/L with the concentration of buffer solution dilution, and the temperature of control reactor is 37 ℃, keeps by regulating in the buffer solution concentration sodium hydroxide that pH is 6.3-6.7 in the fermentation tank, and 12 hours buffer solution time of staying, stir speed (S.S.) is 90 rev/mins.The interpolation speed of bed material is increased to 30g/L/d after 7 days gradually from 10g/L/d.Residue after the degraded is discharged through outlet, is increased to 1000mL/d (being equivalent to 60 hours substrate time of staying) after 7 days gradually from the 250mL/d of beginning.When reaching balance, the degradation efficiency of phyteral rubbish reaches 67%, the production efficiency of short chain organic acid reaches 0.35g/g phyteral organic waste, acetate 0.18g/g phyteral organic waste wherein, propionic acid 0.08g/g phyteral organic waste, butyric acid 0.07g/g phyteral organic waste, remaining is isobutyric acid, valeric acid, isovaleric acid and ethanol etc.Hydrogen content reaches 19% in the gas componant, and hydrogen yield is a 55ml/g phyteral organic waste.
Embodiment 3:
1, the fresh stem or leaf of cattail of harvesting from the pond is broken into the slurry of length less than 8mm with beater.
2, getting the 1200ml rumen fluid from the fistula goat transfers to rapidly in 5 liters of fermentation tanks, the stem or leaf of cattail 80g and the 2.8L mineral matter buffer solution that add pulpous state, keep by regulating in the buffer solution concentration sodium hydroxide that pH is 6.3-6.8 in the fermentation tank, 41 ℃ of temperature, stir speed (S.S.) are 100 rev/mins.The interpolation speed of the pulpous state stem or leaf of cattail remained 20g/L/d, and the time of staying of buffer solution in reactor dropped to the 6th day 16 hours gradually and remain unchanged from 24 hours, and the time of staying of the stem or leaf of cattail in reactor is 60 hours.Detect the composition that produces composition in the gas every day, and when detecting less than methane when having only hydrogen and carbon dioxide in continuous two all gas componants, methanogen is removed fully, and the rumen microorganism domestication is finished.
3, the rumen microorganism after domestication is finished is inoculated in the anaerobic digestion reaction vessel, keeps 2.0L/ branch clock rate to charge into argon gas 20 minutes, drives away residual oxygen in the reactor, guarantees the anaerobic state in the reactor.Overall reactor is amassed 4.9L, and wherein the reaction zone volume is 2.5 liters.The stem or leaf of cattail is 20g/L with the concentration of buffer solution dilution, and the temperature of control reactor is 41 ℃, keeps by regulating in the buffer solution concentration sodium hydroxide that pH is 6.3-6.8 in the fermentation tank, and 16 hours buffer solution time of staying, stir speed (S.S.) is 100 rev/mins.The interpolation speed of bed material is increased to 30g/L/d after 7 days gradually from 10g/L/d.Residue after the degraded is discharged through outlet, is increased to 1000mL/d (being equivalent to 60 hours substrate time of staying) after 7 days gradually from the 250mL/d of beginning.When reaching balance.The degradation efficiency of the stem or leaf of cattail reaches 57%, and the production efficiency of short chain organic acid reaches the 0.37g/g stem or leaf of cattail.The acetate 0.18g/g stem or leaf of cattail wherein, the propionic acid 0.10g/g stem or leaf of cattail, the butyric acid 0.07g/g stem or leaf of cattail, remaining is ethanol, isobutyric acid, valeric acid and isovaleric acid.Hydrogen content is 20% in the gas, the hydrogen yield 50ml/g stem or leaf of cattail.
Claims (1)
1. method of utilizing rumen microorganism to come degrading plant matter discarded object, comprise that (1) is broken or pull an oar into standby bed material with botanical waste, (2) in ruminant tumor gastric, take out rumen microorganism, and be inoculated in the anaerobic digestion reaction vessel that fills botanical waste bed material and mineral matter buffer solution, (3) start the operation anaerobic digestion reaction vessel, make the phyteral hydrolysis acidification, (4) collect gas, liquid product and the solid product that produces; It is characterized in that, describedly in ruminant tumor gastric, take out rumen microorganism, at first must be inoculated into and cultivate in the fermentation tank and tame, its inoculum concentration is the 5-30% of fermentation tank reaction volume, fill botanical waste bed material and mineral matter buffer solution in this fermentation tank, the concentration of phyteral bed material is 10-40g/L; The domestication condition is: the interpolation speed of botanical waste bed material is 20-40g/L/d, the time of staying of mineral matter buffer solution in fermentation tank is 12-24 hour, keep by regulating in the buffer solution concentration sodium hydroxide that pH is 6.0-7.0 in the fermentation tank, temperature is 30-43 ℃, stir speed (S.S.) 50-100 rev/min, the time of staying 48-72 of bed material in fermentation tank hour, when detecting less than methane, inoculate in the anaerobic digestion reaction vessel that fills botanical waste bed material and mineral matter buffer solution and enter the routine operation program up in fermentation tank institute aerogenesis body composition, only detecting hydrogen and carbon dioxide continuous two weeks.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102077089A (en) * | 2008-06-25 | 2011-05-25 | 瓦洛雷克斯公司 | Method for evaluating the amount of methane produced by a dairy ruminant and method for decreasing and controlling this amount |
| CN102284465A (en) * | 2011-04-25 | 2011-12-21 | 华南师范大学 | Biological treatment method for efficiently degrading kitchen garbage grease |
| US8329455B2 (en) | 2011-07-08 | 2012-12-11 | Aikan North America, Inc. | Systems and methods for digestion of solid waste |
| CN103026225A (en) * | 2010-06-29 | 2013-04-03 | 瓦洛雷克斯公司 | Method for evaluating the quantity of methane produced by a ruminant used for meat production |
| CN102056684B (en) * | 2008-06-05 | 2013-08-14 | 威立雅环境服务公司 | Method and plant for processing waste and producing methane |
| CN103484502A (en) * | 2013-09-22 | 2014-01-01 | 大连理工大学 | Method for producing methane by using anerobic fermentation of rumen microorganism |
| CN103798523A (en) * | 2014-03-10 | 2014-05-21 | 南昌资环生态科技有限公司 | Preparation method and application of homological rumen micro-ecological regulating agent |
| CN106086087A (en) * | 2016-08-18 | 2016-11-09 | 吉林中之林农业科技有限公司 | A kind of microbial co culture prepares the system of biomass monomer |
| CN106978446A (en) * | 2017-04-01 | 2017-07-25 | 长兴微羽智能科技有限公司 | A kind of method that utilization stalk prepares hydrogen |
| WO2018033146A1 (en) * | 2016-08-18 | 2018-02-22 | 侯哲生 | System for preparing biomass monomer by means of microbial coculture |
| CN110229851A (en) * | 2019-06-20 | 2019-09-13 | 南京农业大学 | A method of producing methane and acetic acid |
| CN110791533A (en) * | 2019-11-14 | 2020-02-14 | 北京科技大学 | Method for improving acid production of kitchen waste saccharified residue through fermentation by using rumen microorganisms |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8400764A (en) * | 1984-03-09 | 1985-10-01 | Stichting Katholieke Univ | METHOD FOR PRODUCING METHANE FROM SOLID VEGETABLE MATERIAL |
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2004
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102056684B (en) * | 2008-06-05 | 2013-08-14 | 威立雅环境服务公司 | Method and plant for processing waste and producing methane |
| CN102077089B (en) * | 2008-06-25 | 2013-10-23 | 瓦洛雷克斯公司 | Method for evaluating amount of methane produced by dairy ruminant and method for decreasing and controlling this amount |
| CN102077089A (en) * | 2008-06-25 | 2011-05-25 | 瓦洛雷克斯公司 | Method for evaluating the amount of methane produced by a dairy ruminant and method for decreasing and controlling this amount |
| CN103026225A (en) * | 2010-06-29 | 2013-04-03 | 瓦洛雷克斯公司 | Method for evaluating the quantity of methane produced by a ruminant used for meat production |
| CN103026225B (en) * | 2010-06-29 | 2015-07-29 | 瓦洛雷克斯公司 | The appraisal procedure of the methane content produced by the ruminant for the production of meat |
| CN102284465A (en) * | 2011-04-25 | 2011-12-21 | 华南师范大学 | Biological treatment method for efficiently degrading kitchen garbage grease |
| CN102284465B (en) * | 2011-04-25 | 2013-02-13 | 华南师范大学 | Biological treatment method for efficiently degrading kitchen garbage grease |
| US9328323B2 (en) | 2011-07-08 | 2016-05-03 | Aikan North America, Inc. | Systems and methods for digestion of solid waste |
| US8492134B2 (en) | 2011-07-08 | 2013-07-23 | Aikan North America, Inc. | Systems and methods for digestion of solid waste |
| US8329455B2 (en) | 2011-07-08 | 2012-12-11 | Aikan North America, Inc. | Systems and methods for digestion of solid waste |
| CN103484502A (en) * | 2013-09-22 | 2014-01-01 | 大连理工大学 | Method for producing methane by using anerobic fermentation of rumen microorganism |
| CN103484502B (en) * | 2013-09-22 | 2015-04-08 | 大连理工大学 | Method for producing methane by using anerobic fermentation of rumen microorganism |
| CN103798523A (en) * | 2014-03-10 | 2014-05-21 | 南昌资环生态科技有限公司 | Preparation method and application of homological rumen micro-ecological regulating agent |
| CN106086087A (en) * | 2016-08-18 | 2016-11-09 | 吉林中之林农业科技有限公司 | A kind of microbial co culture prepares the system of biomass monomer |
| WO2018033146A1 (en) * | 2016-08-18 | 2018-02-22 | 侯哲生 | System for preparing biomass monomer by means of microbial coculture |
| CN106978446A (en) * | 2017-04-01 | 2017-07-25 | 长兴微羽智能科技有限公司 | A kind of method that utilization stalk prepares hydrogen |
| CN110229851A (en) * | 2019-06-20 | 2019-09-13 | 南京农业大学 | A method of producing methane and acetic acid |
| CN110791533A (en) * | 2019-11-14 | 2020-02-14 | 北京科技大学 | Method for improving acid production of kitchen waste saccharified residue through fermentation by using rumen microorganisms |
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