CN1771916A - Microcapsule preparing process utilizing waste beer yeast cell as wall material - Google Patents
Microcapsule preparing process utilizing waste beer yeast cell as wall material Download PDFInfo
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- CN1771916A CN1771916A CN 200510015618 CN200510015618A CN1771916A CN 1771916 A CN1771916 A CN 1771916A CN 200510015618 CN200510015618 CN 200510015618 CN 200510015618 A CN200510015618 A CN 200510015618A CN 1771916 A CN1771916 A CN 1771916A
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Abstract
The present invention discloses process of preparing microcapsule with waste beer yeast cell as wall material. Waste beer yeast recovered from fermentation plant is prepared into microcapsule through the steps of washing, sieving, centrifuging, etc. to purify; vacuum drying or spray drying to obtain dry yeast grains of 0.5-2 size and water content of 2 %; adding capsule core material in 1-4 times the dry yeast mass and vibrating at 30-80 deg.c for 4-12 hr to make the capsule core material penetrate into yeast cell; washing and drying. The present invention has the advantages of low cost, high core material embedding rate, fast embedding, and simple post washing and drying.
Description
Technical field
The present invention relates to utilize waste beer yeast cell to prepare the method for microcapsule, belong to a kind of microcapsule technology of preparing as the wall material.
Background technology
Microcapsule technology is to utilize natural or synthetic macromolecular compound to prepare microcapsule as filmogen.The material that is enclosed in microcapsule inside is called capsule-core, and the outside film that is formed by filmogen is called cyst wall.By micro encapsulation, can make the core material avoid the influence of external environment condition, reduce volatility, improve stability, and micro encapsulation also has the shielding abnormal flavour, reduces advantages such as toxicity and sustained release.Chemical method, physical method and physico-chemical process three classes are adopted in the preparation of microcapsule usually.But these methods generally all exist preparation process complexity, controlled condition precision prescribed height, cost height, constant product quality is poor, particularly suitable wall material is selected limitation such as difficulty.
The seventies in 20th century, the Shank invention utilizes yeast cells to prepare the technology (U.S., USP 4001480,1977) of microcapsule as natural wall material.Because the spherical in shape or elliposoidal of yeast cells exists with dispersive unicellular state, diameter is between several microns to tens of microns, and cell wall that it is complete and membrane structure have certain intensity and permeability, are ideal wall materials therefore.Compare with traditional microcapsule preparation method, for preparing microcapsule, the wall material has following advantage (Nelson G.Application ofmicroencapsulation in textiles.International Journal of Pharmaceutics.2002 with the yeast cells, 242:55.): in the preparation process, do not need or seldom introduce other chemical reagent, the problem that does not have dissolvent residual or remove is fit to the coating of medicine and food additive very much; The microcapsule product size homogeneous that obtains, nontoxic, biocompatibility good, easily biological-degradable.
But adopt this method to prepare microcapsule, generally used yeast is all had specific (special) requirements: need make in the cell fat content reach 40% (mass fraction) above (U.S., USP4001480,1977) through the specific culture condition as yeast; Yeast cells need keep biological activity (EUROPEAN PATENT OFFICE, EP0242135A2,1987 in the microcapsule preparation process; The U.S., USP 5288632,1994); And normally use with the form of wet thallus, the cell water content height, in bulk, bad dispersibility bond between the yeast cells, the hydration shell of cell surface can influence fat-soluble capsule-core and permeate in yeast cells, and subsequent drying, pelletize operation also relatively difficulty (U.S., USP 4001480,1977; Britain, GP 2162147,1986; EUROPEAN PATENT OFFICE, EP 0085805A1,1982; EUROPEAN PATENT OFFICE, EP 0242135A2,1987; The U.S., USP 5288632,1994).In addition with the yeast cells of fresh cultured as feedstock production microcapsule cost than higher.
Summary of the invention
Prepare the problem that exists in the microcapsule process at the above yeast cells that utilizes as the wall material, the objective of the invention is to propose a kind of method of utilizing waste beer yeast cell to prepare microcapsule as the wall material.
The present invention is achieved by the following technical solutions, it is characterized in that may further comprise the steps:
(1) purification of beer waste yeast: the beer waste yeast that fermentation plant is reclaimed is crossed 60～80 mesh sieves with 3～5 times sterilized water washing, removes the bigger impurity of particle diameter, with the centrifugal 10min of 2000～5000r/min, and the collection yeast; By sterilized water post rinse yeast 1～2 time of same step, up to gained supernatant colorless and odorless, yeast is till the canescence.
(2) yeast drying: get step (1) gained yeast, through vacuum drying or spray drying, make the dry yeast granule of the about 0.5～2mm of diameter, water content is lower than 2%.
(3) microcapsule preparation: take by weighing a certain amount of dry yeast, put into the ground triangular flask, press the capsule-core that the dry yeast mass ratio adds 1～4 times,, put into the water bath with thermostatic control agitator after adding a cover; At 30～80 ℃, frequency of oscillation is under 60～120r/min condition, vibration 4～12h; Sucking filtration reclaims the capsule-core that does not wrap into yeast cells inside; Utilize the residual capsule-core of absolute ethanol washing yeast cell surface; The dry yeast cells that has been embedded with capsule-core in 30～50 ℃ of vacuum driers; Collect yeast cells, promptly make microcapsule.
Above-mentioned core materials is the liquid or solid state, the direct and dry yeast cell contact preparation microcapsule of the capsule-core of liquid; The solid capsule-core need at first be dissolved in dehydrated alcohol or the acetone, and then carries out the microcapsule preparation.The core materials that is embedded in the yeast cells comprises volatile oil, spices and essence and the fat-soluble medicine that extracts from Chinese herbal medicine.
Characteristics of the present invention: for not having specific (special) requirements as fat content in the activity of the yeast cells of microcapsule wall material and the cell; Utilize the waste yeast of fermentation plant, so raw material is cheap and easy to get, the microcapsule preparation cost is reduced greatly; Adopt dry yeast to make the wall material, making the microcapsule preparation process is to carry out in non-water environment, capsule-core embedding rate height, and embedding speed is fast, and subsequent washing, dry run are carried out easily.
The specific embodiment
The beer waste yeast that embodiment 1 reclaims fermentation plant is crossed 80 orders (diameter) sieve with 3 times sterilized water washing, removes the bigger impurity of particle diameter, with the centrifugal 10min of 3000r/min, and the collection thalline; By sterilized water post rinse thalline 2 times of same step; Collect yeast sedimentation, add water and make 10% suspension; Utilize centrifugal type atomizer, rotating speed is 9500r/min, yeast cream is sent into carried out drying in the drying machine, makes the dry yeast granule of the about 0.5～2mm of diameter, and water content is lower than 2%.
Precision takes by weighing the 2g dry yeast, puts into the ground triangular flask, adds 2.0g cinnamic aldehyde liquid, puts into the water bath with thermostatic control agitator after adding a cover; At 50 ℃ (rotating speed 80r/min) vibration 4h, can enter yeast cells inside by the infiltration diffusion, form microcapsule; Sucking filtration also reclaims the cinnamic aldehyde that does not wrap into; The quintessence oil that the absolute ethanol washing cell surface is residual; 30 ℃ of vacuum drying 20min; Collect microcapsule, weigh.The cinnamic aldehyde embedding rate can reach 38.74%.The cinnamic aldehyde embedding rate is meant the ratio (down together) of capsule-core (cinnamic aldehyde) quality with the whole microcapsule quality of embedding in the yeast cells microcapsule.
With nail cover applying light cinnamic aldehyde microcapsule, have one special cinnamic aldehyde abnormal smells from the patient and emit.Under optical microscope, the yeast microcapsule that is embedded with cinnamic aldehyde is the sphere or the elliposoidal of rule, and substantially all between 2.0～6.0 μ m, cell centre can be observed glittering oil droplet and exist for big or small homogeneous, diameter.
The purification of embodiment 2 beer waste yeasts and dry run are with embodiment 1, and precision takes by weighing the 2g dry yeast, put into the ground triangular flask, add the 2g Oleum Caryophylli, put into the water bath with thermostatic control agitator after adding a cover; At 70 ℃ (rotating speed 80r/min) vibration 9h; Sucking filtration also reclaims the Oleum Caryophylli that does not wrap into; The quintessence oil that the absolute ethanol washing cell surface is residual; 30 ℃ of vacuum drying 20min; Collect microcapsule, weigh.The Oleum Caryophylli embedding rate can reach 41.26%.The purification of embodiment 3 beer waste yeasts and dry run are with embodiment 1.Precision takes by weighing the 2.0g dry yeast, puts into the ground triangular flask, adds the 2.5g rosemary ethereal oil, puts into the water bath with thermostatic control agitator after adding a cover; At 60 ℃ (rotating speed 100r/min) vibration 6h, can form microcapsule; Sucking filtration also reclaims the quintessence oil that does not wrap into; The quintessence oil that the absolute ethanol washing cell surface is residual; 30 ℃ of vacuum drying 20min; Collect microcapsule, weigh.The rosemary ethereal oil embedding rate can reach 46.78%.With nail cover applying light rosemary ethereal oil microcapsule, have one strong rosemary ethereal oil and emit.
The purification of embodiment 4 beer waste yeasts and dry run are with embodiment 1.Precision takes by weighing the 2.0g dry yeast, puts into the ground triangular flask, adds the alcoholic solution of 2ml 1mol/L phenyl salicytate, puts into the water bath with thermostatic control agitator after adding a cover; At 70 ℃ (rotating speed 100r/min) vibration 6h, can form microcapsule; The solution that the absolute ethanol washing cell surface is residual; 40 ℃ of vacuum drying 60min; Collect microcapsule, weigh.The phenyl salicytate embedding rate can reach 67.64%.
Claims (3)
1. one kind is utilized waste beer yeast cell to prepare the method for microcapsule as the wall material, it is characterized in that may further comprise the steps:
(1) purification of beer waste yeast: use the sterilized water of 3～5 times of mass ratioes to wash the beer waste yeast that fermentation plant reclaims, cross 60～80 mesh sieves, remove the bigger impurity of particle diameter,, collect yeast with the centrifugal 10min of 2000～5000r/min; By sterilized water post rinse yeast 1～2 time of same step, up to gained supernatant colorless and odorless, yeast is till the canescence;
(2) yeast drying: get step (1) gained yeast, through vacuum drying or spray drying, make the dry yeast granule of the about 0.5～2mm of diameter, water content is lower than 2%;
(3) microcapsule preparation: take by weighing a certain amount of dry yeast, put into the ground triangular flask, press the capsule-core that the dry yeast mass ratio adds 1～4 times, put into the water bath with thermostatic control agitator after adding a cover; At 30～80 ℃, frequency of oscillation is the 4～12h that vibrates under 60～120r/min condition; Sucking filtration reclaims the capsule-core that does not wrap into yeast cells inside; Utilize the residual capsule-core of absolute ethanol washing yeast cell surface; Dry in 30～50 ℃ of vacuum driers; Collect yeast cells, promptly make microcapsule.
2. the waste beer yeast cell that utilizes as claimed in claim 1 prepares the method for microcapsule as the wall material, and the core materials that it is characterized in that being embedded in the dry yeast is the liquid or solid state, and the capsule-core of liquid directly and dry yeast cell contact preparation microcapsule; The solid capsule-core need at first be dissolved in dehydrated alcohol or the acetone, and then carries out the microcapsule preparation.
3. the waste beer yeast cell that utilizes as claimed in claim 1 prepares the method for microcapsule as the wall material, and the core materials that it is characterized in that being embedded in the yeast cells comprises volatile oil, spices and essence and the fat-soluble medicine that extracts from Chinese herbal medicine.
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| CNB2005100156186A CN100389754C (en) | 2005-10-25 | 2005-10-25 | Microcapsule preparing process utilizing waste beer yeast cell as wall material |
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Cited By (10)
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| CN104226211A (en) * | 2014-09-17 | 2014-12-24 | 天津春发生物科技集团有限公司 | Method for preparing essence microcapsules with biology-spray drying method |
| CN104336574A (en) * | 2014-11-10 | 2015-02-11 | 天津市春升清真食品有限公司 | Method for preparing yeast microcapsule by biology-low temperature freeze-drying method |
| CN104759242A (en) * | 2015-03-31 | 2015-07-08 | 浙江工业大学 | Method for synthesizing metal-organic-framework hollow-structured capsule by utilizing saccharomycete |
| CN106578141A (en) * | 2016-12-20 | 2017-04-26 | 乌鲁木齐上善元生物科技有限公司 | Conjugated linoleic acid microcapsule and preparation method thereof |
| CN108017981A (en) * | 2017-12-29 | 2018-05-11 | 唐林元 | A kind of preparation method of quick-drying type metal anticorrosive paint |
| CN109548864A (en) * | 2019-01-10 | 2019-04-02 | 山东理工大学 | A kind of preparation method of slow-release sterilizing anti-staling agent for fruit and vegetable |
| CN110937844A (en) * | 2019-12-02 | 2020-03-31 | 浙江工业大学 | Beer waste yeast microcapsule and application thereof in preparation of self-repairing cement-based material |
| CN112023842A (en) * | 2020-08-29 | 2020-12-04 | 汕头市奇伟实业有限公司 | Thyme essential oil microcapsule, antiseptic composition and preparation method thereof |
| CN113528093A (en) * | 2021-06-18 | 2021-10-22 | 东南大学 | Yeast cell wall-coated phase-change material microcapsule and preparation method and application thereof |
| CN115212184A (en) * | 2022-08-23 | 2022-10-21 | 怀化学院 | Perilla seed oil nano-particles and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB1548022A (en) * | 1976-10-06 | 1979-07-04 | Wyeth John & Brother Ltd | Pharmaceutial dosage forms |
| CN1136834C (en) * | 2001-08-14 | 2004-02-04 | 广东省微生物研究所 | Method for preparing microencapsulated yeast multienzyme cluster |
| CN1589776A (en) * | 2003-09-01 | 2005-03-09 | 中国科学院大连化学物理研究所 | Microencapsulated saccharomyces and its application |
| CN1589777A (en) * | 2003-09-01 | 2005-03-09 | 中国科学院大连化学物理研究所 | Microencapsulated saccharomyces and its application |
| CN1616658A (en) * | 2003-11-10 | 2005-05-18 | 中国科学院大连化学物理研究所 | Method for preparing fixed yeast cell micro capsule |
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| CN104226211A (en) * | 2014-09-17 | 2014-12-24 | 天津春发生物科技集团有限公司 | Method for preparing essence microcapsules with biology-spray drying method |
| CN104336574A (en) * | 2014-11-10 | 2015-02-11 | 天津市春升清真食品有限公司 | Method for preparing yeast microcapsule by biology-low temperature freeze-drying method |
| CN104336574B (en) * | 2014-11-10 | 2019-08-20 | 天津春发生物科技集团有限公司 | A kind of method that biology-frozen drying method prepares yeast microcapsules |
| CN104759242A (en) * | 2015-03-31 | 2015-07-08 | 浙江工业大学 | Method for synthesizing metal-organic-framework hollow-structured capsule by utilizing saccharomycete |
| CN106578141A (en) * | 2016-12-20 | 2017-04-26 | 乌鲁木齐上善元生物科技有限公司 | Conjugated linoleic acid microcapsule and preparation method thereof |
| CN108017981A (en) * | 2017-12-29 | 2018-05-11 | 唐林元 | A kind of preparation method of quick-drying type metal anticorrosive paint |
| CN109548864A (en) * | 2019-01-10 | 2019-04-02 | 山东理工大学 | A kind of preparation method of slow-release sterilizing anti-staling agent for fruit and vegetable |
| CN110937844A (en) * | 2019-12-02 | 2020-03-31 | 浙江工业大学 | Beer waste yeast microcapsule and application thereof in preparation of self-repairing cement-based material |
| CN112023842A (en) * | 2020-08-29 | 2020-12-04 | 汕头市奇伟实业有限公司 | Thyme essential oil microcapsule, antiseptic composition and preparation method thereof |
| CN113528093A (en) * | 2021-06-18 | 2021-10-22 | 东南大学 | Yeast cell wall-coated phase-change material microcapsule and preparation method and application thereof |
| CN115212184A (en) * | 2022-08-23 | 2022-10-21 | 怀化学院 | Perilla seed oil nano-particles and preparation method and application thereof |
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| CN100389754C (en) | 2008-05-28 |
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