CN107048349A - A kind of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent - Google Patents
A kind of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent Download PDFInfo
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- CN107048349A CN107048349A CN201710165371.9A CN201710165371A CN107048349A CN 107048349 A CN107048349 A CN 107048349A CN 201710165371 A CN201710165371 A CN 201710165371A CN 107048349 A CN107048349 A CN 107048349A
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- liquid
- morchella esculenta
- mycelium
- morchellaconica
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- 240000002769 Morchella esculenta Species 0.000 title claims abstract description 30
- 235000002779 Morchella esculenta Nutrition 0.000 title claims abstract description 30
- 150000001875 compounds Chemical class 0.000 title claims abstract description 22
- 150000004676 glycans Chemical class 0.000 claims abstract description 53
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 52
- 239000005017 polysaccharide Substances 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 51
- 241000723418 Carya Species 0.000 claims abstract description 40
- 210000003462 vein Anatomy 0.000 claims abstract description 30
- 241000221639 Morchella conica Species 0.000 claims abstract description 28
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 25
- 239000002002 slurry Substances 0.000 claims abstract description 19
- 238000001035 drying Methods 0.000 claims abstract description 14
- 229920001353 Dextrin Polymers 0.000 claims abstract description 9
- 239000004375 Dextrin Substances 0.000 claims abstract description 9
- 235000019425 dextrin Nutrition 0.000 claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 239000000470 constituent Substances 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 24
- 230000004151 fermentation Effects 0.000 claims description 24
- 235000013339 cereals Nutrition 0.000 claims description 21
- 235000015099 wheat brans Nutrition 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 15
- 229920002472 Starch Polymers 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000008107 starch Substances 0.000 claims description 10
- 235000019698 starch Nutrition 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 244000068988 Glycine max Species 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 claims description 9
- 239000000084 colloidal system Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 238000001802 infusion Methods 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 210000001367 artery Anatomy 0.000 claims description 2
- 239000008400 supply water Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 1
- 239000003292 glue Substances 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 6
- 235000013305 food Nutrition 0.000 abstract description 5
- 239000008187 granular material Substances 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 150000001413 amino acids Chemical class 0.000 abstract description 4
- 230000002929 anti-fatigue Effects 0.000 abstract description 4
- 230000000259 anti-tumor effect Effects 0.000 abstract description 4
- 230000033228 biological regulation Effects 0.000 abstract description 4
- 239000003963 antioxidant agent Substances 0.000 abstract description 3
- 230000003078 antioxidant effect Effects 0.000 abstract description 3
- 235000006708 antioxidants Nutrition 0.000 abstract description 3
- 230000001900 immune effect Effects 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 13
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 11
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 10
- -1 fucose Chemical compound 0.000 description 10
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 9
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 9
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 7
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 7
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 241001494479 Pecora Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000008346 aqueous phase Substances 0.000 description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013402 health food Nutrition 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 2
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 238000001212 derivatisation Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- JEXVQSWXXUJEMA-UHFFFAOYSA-N pyrazol-3-one Chemical compound O=C1C=CN=N1 JEXVQSWXXUJEMA-UHFFFAOYSA-N 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229920001491 Lentinan Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- BQFCCCIRTOLPEF-UHFFFAOYSA-N chembl1976978 Chemical compound CC1=CC=CC=C1N=NC1=C(O)C=CC2=CC=CC=C12 BQFCCCIRTOLPEF-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000019788 craving Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- FYGDTMLNYKFZSV-MRCIVHHJSA-N dextrin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1O[C@@H]1[C@@H](CO)OC(O[C@@H]2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-MRCIVHHJSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229940115286 lentinan Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a kind of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent, the product is made by the constituent of following parts by weight：25 parts of balck vein hickory chick polysaccharide liquid, 36 parts of Morchellaconica polysaccharide liquid, 35 parts of dextrin, 24 parts of FOS；Operation drying is held using vacuum Lapie after the slurry sterilizing being mixed to prepare in preparation；Compound Morchella esculenta (L.) Pers mycelium polyoses grain agent product of the present invention, with the bioactivity such as anti-oxidant, antitumor, immunological regulation, antifatigue；Because containing other materials such as protein, amino acid, enriching the nutrition of granule, being a kind of health-care nutritional food.
Description
Technical field
Processed the present invention relates to the biofermentation of higher fungus and health food, relate generally to balck vein hickory chick mycelium many
Sugar and Morchellaconica compound polyose granule, belong to food and field of health care food.
Background technology
Polysaccharide in animal, plant and fungi often has some specific functions, is one of modern functions food research
Key areas.Wherein, effect of fungi polysaccharide is especially notable, such as well-known lentinan.The source one of fungi polysaccharide
Individual is to have fructification extraction, and this can consume substantial amounts of edible mushroom, and another preferable method is extracted by mycelium, so more
Economical and efficient, but be not that each edible mushroom can cultivate its mycelium.
Hickory chick is a kind of wild rare edible and medicinal fungi, nutritious, rich in protein, polysaccharide, vitamin, amino acid
With several mineral materials and abundant aliphatic acid, local flavor is delicious, very popular.Studies have reported that, hickory chick has drop blood
The effect such as fat, immune, the antifatigue, radioresistance of regulation, antitumor.Due to being limited by hickory chick cultivation technique, hickory chick is wild
Resource-constrained so that hickory chick holds at high price.There are some researches show the Morchella esculenta (L.) Pers mycelium nutritional ingredient of liquid fermentation and culture
It is similar to fructification, thus it is possible to make different hickory chick products to meet people by liquid fermentation large-scale production mycelium
Craving to hickory chick health-care efficacy.
Morchella esculenta (L.) Pers polysaccharide has the bioactivity such as anti-oxidant, antitumor, immunological regulation, antifatigue, enhancing body function, because
This, extraction polysaccharide is paid close attention to by scholars from hickory chick.Conventional method is in the majority with solvent extraction polysaccharide, and it is many that the method is extracted
Sugar has dissolvent residual phenomenon, so as to influence polysaccharide quality and edible safety.
Granule is a kind of physical aspect of product, and the production of some food, health food and medicine usually uses particle
The form of expression of agent.In production technology, generally various materials are mixed, mix is added to swing extruding into half dry material
Extruder grain in comminutor, then wet granular drying is formed.This method products obtained therefrom particle is close, more difficult when being dissolved in water
It is molten, it is more difficult to dissolving when in raw material containing macromolecular colloid materials such as polysaccharide or protein.
The content of the invention
One kind is provided and can be industrialized on a large scale it is an object of the invention to overcome above-mentioned prior art defect
Production, and the higher Morchella esculenta (L.) Pers mycelium polyoses grain agent of effectiveness can be produced at low cost out；Present invention research is found
Vigorous, yield height, if by two kinds is grown using the black tripe hickory chick of liquid fermentation technology culture and Morchellaconica mycelium
Morchella esculenta (L.) Pers polysaccharide, which is combined proportioning, can preferably play the physiologically active of both granuloses.Carried with colloid mill homogenization process
The dissolution rate of high mycelium polysaccharides, solves solvent extraction polysaccharide problem of solvent residual, meanwhile, make full use of other in mycelium
Nutritional ingredient, produce nutritious granule；In production technology, realize that Lapie holds operation, institute to slurry using vacuum
Obtain and many holes are produced inside particle, specific volume reduces, be conducive to moisture rapid osmotic during dissolving to promote dissolving, instant edible.
Compound Morchella esculenta (L.) Pers mycelium polyoses grain agent of the present invention is made by the constituent of following parts by weight：Balck vein hickory chick is more
2-5 parts of liquid glucose, 3-6 parts of Morchellaconica polysaccharide liquid, 3-5 parts of dextrin, 2-4 parts of FOS；In production technology, vacuum is utilized
Lapie is carried out to slurry and holds many holes of generation inside operation drying, gained particle, specific volume reduces, and is conducive to moisture fast during dissolving
Speed infiltration is to promote to dissolve, instant edible.
Wherein balck vein hickory chick polysaccharide liquid and Morchellaconica polysaccharide liquid are by by balck vein hickory chick strain and pinnacle sheep tripe
Bacterium strain is activated respectively, and mycelium is obtained through liquid deep layer fermenting；Mycelium is scrubbed, colloid mill is homogenized and is made.
The present invention through the following steps that realize：
（1）Prepare liquid deep layer fermenting culture medium：Wheat bran 30g/L-50g/L, soluble starch 15g/L-25 g/L, analysis for soybean powder
8g/L-12 g/L, sucrose 8g/L-12 g/L, wherein wheat bran filter to obtain wheat bran juice, in wheat bran with a certain amount of distilled water infusion
Soluble starch, analysis for soybean powder, sucrose are added in juice, mixing is supplied water, stirred, dispenses, 250mL conical flask liquid amounts
160mL, 121 DEG C of sterilizing 30min；
（2）Actication of culture：The balck vein hickory chick strain and Morchellaconica strain of preservation are inoculated in PDA culture medium, 25 respectively
3-5d is cultivated in DEG C constant incubator, it is standby；
（3）Fermented and cultured：Strain after activation is inoculated in liquid deep layer fermenting culture medium, 21 DEG C -29 DEG C of fermentation temperature,
4d-12d is cultivated under fermentation rotating speed 150r/min-180r/min；
（4）Fermentation liquor treatment：Take step（3）Obtained zymotic fluid, uses two layers of filtered through gauze, mycelium distilled water cyclic washing
3-5 times, mycelium is homogenized with colloid mill, obtains mycelia somaplasm, as balck vein hickory chick polysaccharide liquid and Morchellaconica polysaccharide liquid；
（5）The preparation of Morchella esculenta (L.) Pers mycelium polyoses grain agent：By weight, 2-5 parts of balck vein hickory chick polysaccharide liquid, pinnacle sheep are taken
3-6 parts of tripe granulose liquid, 3-5 parts of dextrin, 2-4 parts of FOS, mix to obtain slurry, and slurry is mixed and 80-90 DEG C is heated to
Keep sterilizing for 3-5 minutes, be cooled to normal temperature, be sufficiently stirred for being mixed into air, sabot is put into vacuum drying chamber and taken out in 55-65 DEG C
Vacuum 4-6 minutes, makes it produce bubble and evaporation section moisture, improves slurry viscosity, releases vacuum, slurry is stirred again
Mix and be mixed into air, be then placed in drying box and be dried in vacuo in 55-65 DEG C, slurry reexpands to form inner porosity
And drying is completed, crushed after being dried, packaging produce compound Morchella esculenta (L.) Pers mycelium polyoses grain agent product.
Balck vein hickory chick strain and Morchellaconica strain are conventional commercial strain in the present invention.
The beneficial effects of the invention are as follows：
（1）Using the inventive method, Morchella esculenta (L.) Pers mycelium polyose is set to develop and improve hickory chick with industrialized production
Mycelial value；
（2）It is homogenized using colloid mill, polysaccharide is fully discharged in mycelial cell, biological effectiveness is more notable；
（3）The present invention research show, the artificial culture biomass of mycelium of Morchellaconica and balck vein hickory chick（Yield）It is high
In the hickory chick of other kinds, therefore with industrialized developing value；
（4）Two kinds of hickory chicks are combined so that polysaccharide component is more in polyose, and content is higher, and effect is more preferable；The present invention's
Research has shown that：Polysaccharide containing different molecular weight in Morchellaconica mycelium, using gel filteration determining wherein content compared with
The molecular weight of two kinds of high holosaccharides PA-1 and PA-2, PA-1 molecular weight is that 24.38KD, PA-2 molecular weight are 87.03KD；It is black
Content higher two kinds of polysaccharide PB-1 and PB-2 are isolated in wheat Morchella esculenta (L.) Pers mycelium, are divided using gel filteration determining PB-1
Son amount is that 13.00KD, PB-2 molecular weight are 39.54KD；Further determine tetra- kinds of PA-1 and PA-2, PB-1 and PB-2 simultaneously
The monose composition of holosaccharide；Measurement result shows：In PA-1 containing mannose, glucose, galactolipin, xylan, arabinose,
6 kinds of monose such as fucose, PA-2 contains 7 kinds of mannose, rhamnose, glucose, galactolipin, xylose, arabinose, fucose etc.
Monose, PB-1 and PB-2 are containing mannose, glucose, galactolipin, xylose, fucose this five kinds of monose；
（5）In polyose, the not only Morchella esculenta (L.) Pers polysaccharide containing Multiple components, also containing the nutrition such as protein and amino acid into
Point, it is a kind of nutraceutical with healthcare function；
（6）Lapie is carried out to slurry using vacuum and holds many holes of generation inside operation, gained particle, specific volume reduces, during dissolving
Be conducive to moisture rapid osmotic to promote dissolving, instant edible；
Compound Morchella esculenta (L.) Pers mycelium polyoses grain agent product of the present invention, with anti-oxidant, antitumor, immunological regulation, antifatigue etc.
Bioactivity；Because containing other materials such as protein, amino acid, enriching the nutrition of granule, being a kind of nutritional health food.
Brief description of the drawings
The HPLC that Fig. 1 is polysaccharide PA-1 in Morchellaconica determines collection of illustrative plates；
The HPLC that Fig. 2 is polysaccharide PA-2 in Morchellaconica determines collection of illustrative plates；
The HPLC that Fig. 3 is polysaccharide PB-1 in balck vein hickory chick determines collection of illustrative plates；
The HPLC that Fig. 4 is polysaccharide PB-2 in balck vein hickory chick determines collection of illustrative plates.
Embodiment
Specific embodiment of the present invention is given below, and the invention will be further described, but protection scope of the present invention is not limited
In the content.
Embodiment 1：The Morchella esculenta (L.) Pers mycelium polyoses grain agent of this compound consists of thing and is made：Balck vein hickory chick polysaccharide
Liquid 2g, Morchellaconica polysaccharide liquid 3g, dextrin 3g, FOS 2g.
（1）Prepare liquid deep layer fermenting culture medium：Wheat bran 30g, soluble starch 15g, analysis for soybean powder 8g, sucrose 8g；Wherein
30g wheat bran 500mL distilled water infusion, filters to obtain wheat bran juice, and soluble starch, analysis for soybean powder, sucrose are added in wheat bran juice,
Mixing, supplies water to 1000mL, stirs, and dispenses, 250mL conical flask liquid amounts 160mL, 121 DEG C of sterilizing 30min；
（2）Actication of culture：The balck vein hickory chick strain and Morchellaconica strain of preservation are inoculated in PDA culture medium, 25 respectively
3d is cultivated in DEG C constant incubator, it is standby；
（3）Fermented and cultured：Two strains after activation are inoculated in liquid deep layer fermenting culture medium respectively, wherein black arteries and veins sheep tripe
The fermentation condition of bacterium is：23 DEG C of fermentation temperature, ferment rotating speed 160r/min, cultivates 8d, pH7；The fermentation condition of Morchellaconica
For：23 DEG C of fermentation temperature, ferment rotating speed 180r/min, cultivates 12d, pH9；
（4）Fermentation liquor treatment：The zymotic fluid for taking step (3) to obtain, uses two layers of filtered through gauze, mycelium distilled water cyclic washing
3 times, mycelium is homogenized with colloid mill, obtains mycelium polysaccharides liquid；
（5）The preparation of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent：Take balck vein hickory chick polysaccharide liquid 2g, Morchellaconica polysaccharide liquid
3 g, the g of dextrin 3, the g of FOS 2, are well mixed and are heated to 80 DEG C of holdings and sterilize for 3 minutes, be cooled to normal temperature, be sufficiently stirred for
Air is mixed into, sabot is put into vacuum drying chamber and vacuumized 5 minutes in 60 DEG C, it is produced bubble and evaporation section moisture, carry
High phosphoric acid, releases vacuum, slurry is stirred is mixed into air again, is then placed in drying box and carries out vacuum in 60 DEG C
Dry, slurry reexpands to form inner porosity and complete drying, and crushed after being dried, packaging produce compound Morciiella Esculeuta Mycelia
Body polyoses grain agent product.After measured, many sugar amounts that every 100g products contain are 227mg.
Embodiment 2：The Morchella esculenta (L.) Pers mycelium polyoses grain agent of this compound consists of thing and is made：Balck vein hickory chick polysaccharide
Liquid 5g, Morchellaconica polysaccharide liquid 4g, dextrin 5g, FOS 3g.
（1）Prepare liquid deep layer fermenting culture medium：Wheat bran 40g, the g of soluble starch 20, the g of analysis for soybean powder 10, the g of sucrose 10；
40g wheat bran 500mL distilled water infusions after wherein weighing, filter to obtain wheat bran juice, and soluble starch, Huang are added in wheat bran juice
Bean powder, sucrose, mixing, supply water to 1000mL, stir, and dispense, 250mL conical flask liquid amounts 160mL, 121 DEG C go out
Bacterium 30min；
（2）Actication of culture：The balck vein hickory chick strain and Morchellaconica strain of preservation are inoculated in PDA culture medium, 25 respectively
4d is cultivated in DEG C constant incubator, it is standby；
（3）Fermented and cultured：Strain after activation is inoculated in liquid deep layer fermenting culture medium, wherein the fermentation of balck vein hickory chick
Condition is：27 DEG C of fermentation temperature, ferment rotating speed 180r/min, cultivates 8d, pH9；The fermentation condition of Morchellaconica is：Fermentation temperature
23 DEG C of degree, ferment rotating speed 180r/min, cultivates 10d, pH8；
（4）Fermentation liquor treatment：The zymotic fluid for taking step (3) to obtain, uses two layers of filtered through gauze, mycelium distilled water cyclic washing
4 times, mycelium is homogenized with colloid mill, obtains mycelium polysaccharides liquid；
（5）The preparation of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent：By weight, balck vein hickory chick polysaccharide liquid 4g, pinnacle sheep are taken
Tripe granulose liquid 4g, dextrin 5g, FOS 3g, are well mixed and are heated to 85 DEG C of holdings and sterilize for 4 minutes, be cooled to normal temperature,
It is sufficiently stirred for being mixed into air, sabot is put into vacuum drying chamber and vacuumized 4 minutes in 55 DEG C, it is produced bubble and evaporation part
Divide moisture, improve phosphoric acid, release vacuum, slurry is stirred is mixed into air again, be then placed in drying box in 55
DEG C it is dried in vacuo, slurry reexpands to form inner porosity and complete drying, and crushed after being dried, packaging produce compound
Morchella esculenta (L.) Pers mycelium polyoses grain agent product.After measured, many sugar amounts that every 100g products contain are 192mg.
Embodiment 3：The Morchella esculenta (L.) Pers mycelium polyoses grain agent of this compound consists of thing and is made：Balck vein hickory chick polysaccharide
Liquid 3g, Morchellaconica polysaccharide liquid 6g, dextrin 4g, FOS 4g.
（1）Prepare liquid deep layer fermenting culture medium：Wheat bran 50g, the g of soluble starch 25, the g of analysis for soybean powder 12, the g of sucrose 12；
The distilled water infusion of wheat bran 500mL after weighing, filters to obtain wheat bran juice, in wheat bran juice add soluble starch, analysis for soybean powder,
Sucrose, mixing, supplies water to 1000mL, stirs, and dispenses, 250mL conical flask liquid amounts 160mL, 121 DEG C of sterilizings
30min；
（2）Actication of culture：The balck vein hickory chick strain and Morchellaconica strain of preservation are inoculated in PDA culture medium, 25 respectively
5d is cultivated in DEG C constant incubator, it is standby；
（3）Fermented and cultured：Strain after activation is inoculated in liquid deep layer fermenting culture medium, wherein the fermentation of balck vein hickory chick
Condition is：23 DEG C of fermentation temperature, ferment rotating speed 180r/min, cultivates 10d, pH8；The fermentation condition of Morchellaconica is：Fermentation
25 DEG C of temperature, ferment rotating speed 160r/min, cultivates 8d, pH8；
（4）Fermentation liquor treatment：The zymotic fluid for taking step (3) to obtain, uses two layers of filtered through gauze, mycelium distilled water cyclic washing
5 times, mycelium is homogenized with colloid mill, obtains mycelium polysaccharides liquid；
（5）The preparation of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent：By weight, balck vein hickory chick polysaccharide liquid 3g, pinnacle sheep are taken
Tripe granulose liquid 6g, dextrin 4g, FOS 4g, are well mixed and are heated to 90 DEG C of holdings and sterilize for 5 minutes, be cooled to normal temperature,
It is sufficiently stirred for being mixed into air, sabot is put into vacuum drying chamber and vacuumized 6 minutes in 65 DEG C, it is produced bubble and evaporation part
Divide moisture, improve phosphoric acid, release vacuum, slurry is stirred is mixed into air again, be then placed in drying box in 65
DEG C it is dried in vacuo, slurry reexpands to form inner porosity and complete drying, and crushed after being dried, packaging produce compound
Morchella esculenta (L.) Pers mycelium polyoses grain agent product.After measured, many sugar amounts that every 100g products contain are 208mg.
The monose composition measuring method and result of Morchella esculenta (L.) Pers polysaccharide in above-described embodiment：
1st, assay method
The derivative of monose mark product（PMP derivatization methods）：10mg monose mark product are weighed respectively, be dissolved in 5mL pure water, 10 are diluted respectively
Times, 1mL0.5mol/LPMP agent is added thereto（The pyrazolone of 1- phenyl -3- methyl -5）, 1mL 0.3mol/L NaOH solutions,
In 30min derived from 70 DEG C of baking ovens, room temperature is cooled to, 1mL 0.3mol/L Hcl solution is added and neutralizes.Then, it is separately added into
3mL chloroforms shake, and stand, discard chloroform phase, the chloroform for adding same volume extracts three times to upper strata aqueous phase repeatedly
It is colourless, by aqueous phase with after 0.45 μm of organic membrane filtration, analyzed for HPLC sample introductions.
The derivative of sample（PMP derivatization methods）：Polysaccharide 10mg after purification is accurately weighed respectively in ampere bottle, is added
2mol/L trifluoroacetic acid 2mL, uses alcohol blast burner tube sealing after mixing, 4h is hydrolyzed in 110 DEG C of baking ovens, is cooled down after taking-up, uses
0.3mol/L NaOH solutions adjust pH to neutrality, are settled to 10mL, centrifuge 10min in 5000r/min, take 1mL supernatants to add respectively
Enter 0.5mol/LPMP agent（The pyrazolone of 1- phenyl -3- methyl -5）Neutralized with each 1mL of 0.3mol/L Hcl.Then, it is separately added into
3mL chloroforms shake, and stand, discard chloroform phase, the chloroform for adding same volume extracts three times to upper strata aqueous phase repeatedly
It is colourless, by aqueous phase with after 0.45 μm of organic membrane filtration, analyzed for HPLC sample introductions.
Chromatographic condition：Mobile phase, solvent orange 2 A（The phosphate buffer of 15% acetonitrile+85%（0.05mol/L KH2PO4-NaOH,
pH6.9））, solvent B（The phosphate buffer of 40% acetonitrile+60%（0.05mol/L KH2PO4-NaOH, pH6.9））；Chromatographic column,
Sephax C18-H；Gradient mode, 0 → 35min, respective concentration gradient is 0% → 40% solvent B；Flow velocity, 1mL/min；Detection
Wavelength, 250nm；Sample size, 20 μ L；Column temperature, 25 DEG C.
Testing result is shown in Fig. 1,2,3,4；The monose composition for showing PA-1 in Fig. 1 is mannose, glucose, galactolipin, wood
Glycan, arabinose, fucose, its proportion of composing are 12.59:103.69:13.58:12.41:10:137.76；PA-2 in Fig. 2
Monose composition be mannose, rhamnose, glucose, galactolipin, xylose, arabinose, fucose, its proportion of composing be 55:
10:19.6:44.7:18.2:34.2:25.3；In Fig. 3 PB-1 contain mannose, glucose, galactolipin, xylose, fucose this five
Monose is planted, its mol ratio is 0.80: 0.20: 1.36: 0.21: 1.00；PB-2 contains mannose, glucose, gala in Fig. 4
This five kinds of monose of sugar, xylose, fucose, its mol ratio is 1.61: 0.45: 1.00: 0.43: 1.16.
Claims (4)
1. a kind of compound Morchella esculenta (L.) Pers mycelium polyoses grain agent, it is characterised in that be made by the constituent of following parts by weight：Black arteries and veins
2-5 parts of Morchella esculenta (L.) Pers polysaccharide liquid, 3-6 parts of Morchellaconica polysaccharide liquid, 3-5 parts of dextrin, 2-4 parts of FOS, using true in preparation
Empty Lapie holds operation drying.
2. compound Morchella esculenta (L.) Pers mycelium polyoses grain agent according to claim 1, it is characterised in that balck vein hickory chick polysaccharide
Liquid and Morchellaconica polysaccharide liquid are sent out by the way that balck vein hickory chick strain and Morchellaconica strain are activated respectively through deep liquid
Ferment obtains mycelium；Mycelium is scrubbed, colloid mill is homogenized and is made.
3. compound Morchella esculenta (L.) Pers mycelium polyoses grain agent according to claim 2, it is characterised in that balck vein hickory chick polysaccharide
Liquid and Morchellaconica polysaccharide liquid are specifically prepared according to the following steps：
（1）Prepare liquid deep layer fermenting culture medium：Wheat bran 30g/L-50g/L, soluble starch 15g/L-25 g/L, analysis for soybean powder
8g/L-12 g/L, sucrose 8g/L-12 g/L；Wherein wheat bran first uses distilled water infusion, filters to obtain wheat bran juice, adds in wheat bran juice
Enter soluble starch, analysis for soybean powder, sucrose, mix, supply water, stir, dispense, sterilizing；
（2）Actication of culture：Balck vein hickory chick strain and Morchellaconica strain are inoculated in PDA plate culture medium respectively, 25
Incubated 3-5d at DEG C；
（3）Fermented and cultured：Strain after activation is inoculated in liquid deep layer fermenting culture medium, 21 DEG C -29 DEG C of fermentation temperature,
4-12d is cultivated under rotating speed 150r/min-180r/min；
（4）Fermentation liquor treatment：Take step（3）Obtained filtering fermentation liquor, mycelium distilled water cyclic washing 3-5 times, uses glue
Body mill homogenate, obtains balck vein hickory chick polysaccharide liquid and Morchellaconica polysaccharide liquid.
4. compound Morchella esculenta (L.) Pers mycelium polyoses grain agent according to claim 1, it is characterised in that vacuum Lapie holds operation
It is after the slurry sterilizing for being mixed to prepare constituent, to be sufficiently stirred for being mixed into air, sabot is put into vacuum drying chamber in 55-65
DEG C vacuumize 4-6 minutes, it is produced bubble and evaporation section moisture, improve slurry viscosity, release vacuum, slurry is entered again
Row stirring is mixed into air, is then placed in drying box and is dried in vacuo in 55-65 DEG C, slurry reexpands to form internal porous
Structure simultaneously completes drying, crushed after being dried, packaging, produces compound Morchella esculenta (L.) Pers mycelium polyoses grain agent product.
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| CN108815180A (en) * | 2018-05-23 | 2018-11-16 | 安徽大学 | PM 2.5-induced lung injury antagonist and preparation method thereof |
| CN109336987A (en) * | 2018-08-30 | 2019-02-15 | 吉林大学 | A kind of hickory chick and bolete compound Thick many candies and preparation method and medical application |
| CN109329894A (en) * | 2018-09-18 | 2019-02-15 | 江苏农林职业技术学院 | A kind of hickory chick granule and preparation method thereof |
| CN110463987A (en) * | 2019-09-23 | 2019-11-19 | 襄阳职业技术学院 | A kind of hickory chick granular preparation and preparation method |
| CN112094359A (en) * | 2020-09-28 | 2020-12-18 | 广东轻工职业技术学院 | Extraction method of morchella polysaccharide, morchella polysaccharide drink and preparation method of morchella polysaccharide drink |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108815180A (en) * | 2018-05-23 | 2018-11-16 | 安徽大学 | PM 2.5-induced lung injury antagonist and preparation method thereof |
| CN109336987A (en) * | 2018-08-30 | 2019-02-15 | 吉林大学 | A kind of hickory chick and bolete compound Thick many candies and preparation method and medical application |
| CN109329894A (en) * | 2018-09-18 | 2019-02-15 | 江苏农林职业技术学院 | A kind of hickory chick granule and preparation method thereof |
| CN110463987A (en) * | 2019-09-23 | 2019-11-19 | 襄阳职业技术学院 | A kind of hickory chick granular preparation and preparation method |
| CN112094359A (en) * | 2020-09-28 | 2020-12-18 | 广东轻工职业技术学院 | Extraction method of morchella polysaccharide, morchella polysaccharide drink and preparation method of morchella polysaccharide drink |
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