CN1761867A - Analysis device and method for cell count in the analysis device - Google Patents
Analysis device and method for cell count in the analysis device Download PDFInfo
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- CN1761867A CN1761867A CN 200480007270 CN200480007270A CN1761867A CN 1761867 A CN1761867 A CN 1761867A CN 200480007270 CN200480007270 CN 200480007270 CN 200480007270 A CN200480007270 A CN 200480007270A CN 1761867 A CN1761867 A CN 1761867A
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Abstract
It is possible to optimally control window movement by storing in a window memory the information indicating that movement has been performed by a distance in accordance with the window size, thereby preventing duplicate detection of the same cell. There is no need of acquiring data each time the window size is modified. Thus, it is possible to provide a cell analysis device capable of obtaining a desired number of cells by one data acquisition and performing a highly-accurate analysis in a short time.
Description
Technical field
The present invention also belongs to the technology of analysis of cells in field of medical, relate to the method for cell count in analytical equipment and the analytical equipment, it is the sample that injects the cell that contains various size on disc, with this disc of rayed, come the cell in the test carried out according to its reflection of light or transmitted light that cell size is differentiated and counting.
Background technology
In field of medical, the cell size is differentiated and the conventional art 1 of the analytical equipment of counting usefulness as the cell in the sample is carried out, also use a kind of analytical equipment that utilizes CD, in this analytical equipment, light source is simultaneously followed the tracks of on the track of disc, one in the face of injecting the sample irradiates light on the disc, and detecting device detects its reflected light or transmitted light.The signal that detects is kept in the memory buffer by AD converter.The calibration marker that has expression sense of rotation benchmark on disc, the data that detecting device detects are designated benchmark with correction and form sequence.When not having cell in sample, the light intensity that detecting device detects be certain, and different therewith is, when having cell, because interference of light, the level that detecting device detects reduces, by discern the level variation of such detecting device, judge have acellular.
In addition, this method is to judge have acellularly with one dimension (on the track), with Fig. 1 and Fig. 2 the method for judging cell with two dimension is described below.
Fig. 1 represents the key diagram of the analytical approach of analytical equipment in the past, Fig. 2 represent from toward analytical equipment analyze the key diagram of the method for the cell vary in size.
At first, judge to have acellularly with one dimension, when being judged as cell, each cell storage is one ' 1 ' in storer, be judged as when not having cell, in storer every certain sampling interval storage ' 0 '.The state of memory inside at that time as shown in Figure 1.The one dimension cell recognition data of the cell 104 that exists on the disc track 102 are to make the corresponding form storage of each road 102 and each bit of data bus on the sample storer 101.At this moment, on sample storer 101 configuration and the big or small corresponding m of cell 104 capable * window 103 of n row, tangentially reach the orbital direction displacement of a bit of a bit of one side respectively, one side is scanning on aforementioned memory 101.When scanning, when all row in window 103 existed ' 1 ', being judged as in two dimension had cell.At this moment, whole ' 1 ' be rewritten as ' 0 ' with what exist in the window, this is the processing of carrying out for the same cell of duplicate detection after preventing.At this moment.Size as the cell of detected object is decided by window size.When wanting to change the cell that will detect big or small, can change the size of window 103 in view of the above.
In addition, as the conventional art 2 of analytical equipment, below with description of drawings a kind of method for cell count in the past, it is that the cell of analyzing the various size of injecting on the disc of formation is counted the cell of the size of certain limit respectively by size.
Figure 11 represents the key diagram of the cell detection method in the past the method for cell count, the determination object object of analyzing in Figure 11 (a) expression cell computing method in the past on the disc is the key diagram of the position relation of cell and track and laser, and Figure 11 (b) is illustrated in and uses window to differentiate the size of cell, and the key diagram of the method counted respectively by size in the past the method for cell count.
In Figure 11 (a), the 201st, analyzing the determination object object that injects on the disc is cell, the 202nd, analyze the track on the disc, the 203rd, the laser that on analysis disc, relatively moves.Analytical equipment in the past is to analyze disc injection sample, in the cell 201 of the various size of formation that in sample, exists, analyze the number of specific cells, in such analytical equipment, analyze on the disc identical with CD such as CD-ROM, be carved with spiral track 202, when analyzing the disc rotation, control, make laser 203 on track 202, relatively move.
On the other hand, the determination object object is the width of cell 201 greater than track 202, exists across many tracks 202, and whether when laser 203 is mobile on track 202, depending on has cell 201 on the track 202, produces signal in the laser light receiving unit and changes.By this signal transformation is handled, when being judged to be cell 201, " 1 " is deposited in storer, when situation in addition, " 0 " is deposited in storer,, detect " 1 " length longitudinally based on these data arrays, differentiate by so a plurality of cells being carried out size, and carry out a counting number by size respectively.
As the size differentiation of carrying out cell like this and by the method for cell count that its size is counted cell respectively is to adopt following method, promptly adopt square window, want the size of trying to achieve for each, switch window, the detection assay target object is cell and counts respectively by size.
In above such method for cell count, for example from a plurality of cells of 1～11 size of occupied orbital, when wanting to detect the number of cells of 6 sizes of occupied orbital, shown in Figure 11 (b), at first use the window of 6 * X1 size, along the displacement one by one of directions X one side, one side scans, and each capable number that all comprises the place of " 1 " of window is counted.
Then, with the window of the size of 7 * X1, along the displacement one by one of directions X one side, one side scans, and the number that each row of window is all comprised the position of " 1 " is counted.
By like this, obtain across the cell that exists more than or equal to 6 tracks and across the number of the cell that exists more than or equal to 7 tracks, can obtain the number of the cell that occupies 6 track sizes according to its difference.
In addition, here X1 is the round values that adopts greater than the offset scope of and " 1 " that signal detection errors cause inhomogeneous because of the disc rotation, even the position of " 1 " is offset in each track, also can detect as " 1 " that goes out from same cell detection.
In addition, conventional art 3 as analytical equipment, be to adopt Outlier Detection wave filter FD to detect isolated point to view data, in the regulation zone, utilize the number of the isolated point that detects, whether differentiate view data is halftone dot image, export it according to the halftone dot image method of discrimination and differentiate the result,, in storer, carry out window scanning for view data is handled.
When Figure 16 represents cell recognition to the date storage method in the storer.In Figure 16, every track 312 of disc is obtained the data of the cell 311 of determination object, make the position of data after its binaryzation and data bus corresponding, deposit memory area 313 in according to the order of sampling.Here,, then store,, then store as " 0 " if cell is unrecognized as " 1 " if cell is identified on the track 312 that passes through.
As shown in figure 17, differentiate and counting mode, at first as previously mentioned, in having stored the cell memory of data, the fixed-size window 314 of a * b (being 3 * 8 as an example in the drawings) is scanned as the cell size here.Here, a is the situation of approximately occupying a bar track size corresponding to the cell size of determination object, b is based on following situation, promptly when being offset because of the sample position in the track of beating, if be same cell and also can searching in the continuous b sample at the recognition data " 1 " of cell.In addition, as scan method, be to make sample of the each skew of window, and make window offset downward one at every turn along the position direction along address direction.This method is, if " 1 " has a position continuously on the position direction in the window, then its identification is counted as a cell, and " 1 " in this window all is replaced into " 0 ", repeats these operations then.
But, as shown in Figure 2, in conventional art 1 as described above, in sample, mix to have and want that the cell that detects and size are under the situation of the cell 106 that detects of the twice of cell 105 and not wanting, when scanning, do not want that the cell 106 that detects wants that as two the cell 105 that detects counts with the 107 pairs of sample storeies 101 of window that meet the size of wanting the cell 105 that detects.For example, when not wanting that in cell 105 100 of the existence of wanting to detect there are 50 in the cell 106 that detects,, then become 100+50 * 2=200 such result if detect with window 107.
In order to obtain the quantity of wanting the cell 105 that detects, just must obtain the quantity of not wanting the cell 106 that detects, from sum, deduct again.For this reason, now will be to scanning with the window 108 that meets the size of not wanting the cell 106 that detects in the sample storer 101.But,, therefore can not utilize because the data in the test storer 101 are rewritten after with aforementioned window 107 scannings.For this reason, must obtain data once more again.But the problem that exists like that is to be doubled analysis time, and because analysis condition is inequality, might enlarge analytical error.
In addition, in the assay method that the method for cell count that utilizes conventional art 2 carries out, the window after the counting moves, and the offset of " 1 " of and each track big at X1 hour might repeat to read once detected sequence.
In addition, when wanting to detect the cell that occupies 6 track sizes,, in window scanning next time, will be detected once more for example even occupy the cell that the cell of 7 tracks and above size was once detecting.
Therefore, in method for cell count in the past, about with the windows detecting and the position of once having counted, by " 1 " is transformed to " 0 ", just can not repeat to read as " 1 ", but therefore when the size of switch window detects, must utilize and once more all row be carried out window and scan, redeterminate whether cell is arranged on the track, the problem of existence is that mensuration will be spent more time-consuming.
In addition, in the method for conventional art 3, because using the cell size for target is the window of fixing 314, each cell detection will be rewritten as " 0 " in the storer, therefore for the bigger cell of size, then as shown in figure 18, must change window size is window 315, at this moment owing to can not utilize storer again, therefore must sample again.Therefore the problem that exists is, because condition determination is inequality, so might enlarge counting error, also spends more minute.
Therefore, the object of the present invention is to provide the method for cell count in a kind of analytical equipment and the analytical equipment, it is for the cell on the track, do not need repeatedly to redeterminate whether this cell is arranged, can obtain by a secondary data, short time and differentiate cell size accurately and counting, can improve the counting precision of desirable cell, shorten minute simultaneously.
Summary of the invention
The described analytical equipment in the present invention the 1st aspect, be inject on the disc contain the sample of cell and to this disc irradiates light after obtain the analytical equipment of the cell number of sample according to this reflection of light or transmitted light, have: the one dimension cell recognition unit that carries out cell recognition according to the variation of described reflection of light light or transmitted light with one dimension; Storage is illustrated in and coils the examination storer that the living corresponding bit in each road has acellular the 1st data to use according to the recognition result of described one dimension cell recognition unit; To the window with any size on the described sample storer is that unit scans, comes the two-dimentional cell decision unit with two-dimentional recognizing cells by confirming described the 1st data; It is that unit is attached to the data extra cell in the described sample storer with each window that the expression that utilizes described two-dimentional cell recognition to discern is had acellular the 2nd data; Utilize described the 2nd data to differentiate the cell size judgement unit of cell size; And control the window mobile control unit that described window moves, it constitutes size and the number thereof of obtaining cell by described each window of sample storer additional representation being had acellular the 2nd data, utilizing a secondary data to obtain.
In addition, the described analytical equipment in the present invention the 2nd aspect, be inject on the disc contain the sample of cell and to this disc irradiates light after obtain the analytical equipment of the cell number of sample according to this reflection of light or transmitted light, have: the one dimension cell recognition unit that carries out cell recognition according to the variation of described reflection of light light or transmitted light with one dimension; Storage is illustrated in the sample storer that the corresponding bit in each road with disc has acellular the 1st data to use according to the recognition result of described one dimension cell recognition unit; To the window with any size on the described sample storer is that unit scans, comes the two-dimentional cell recognition unit with two-dimentional recognizing cells by confirming described the 1st data; In the scanning of described test storer, switch the window switch unit of described window size arbitrarily; Differentiate the big or small judgement unit of cell of the cell size of discerning according to the scanning result that uses one or two and plural window size with described two-dimentional cell recognition unit; And in the data delete unit of differentiating deletion described the 1st data in back with described cell size judgement unit, it constitutes when cell has been confirmed in the scanning that utilizes described sample storer, scan again and differentiate the cell size by changing window size, size and the number thereof utilizing a secondary data to obtain to obtain cell.
In addition, the described analytical equipment in the present invention the 3rd aspect is aspect the 1st or the described analytical equipment in the 2nd aspect in, make the employing cycle to change according to the cell in described sample size.
In addition, the described analytical equipment in the present invention the 4th aspect, be in the described analytical equipment in the 1st aspect, the cell compartment storer at the interval that has when described sample storer being scanned, exists between torage cell and the cell with described window, and have when switching described window size and once more the sample storer is scanned, according to only carrying out swept memory and jump over control module in the zone that exists of pair cell from described cell compartment canned data.
According to the above, can obtain by a secondary data and obtain desirable cell number, can analyze accurately with the short time.
In addition, method for cell count in the described analytical equipment in the present invention the 5th aspect, to carry out planar alignment with horizontal X and vertical Y according to the two-value data of " 0 " or " 1 " that has or not the cell of analyzing the multiple size of injecting on the disc of formation to obtain, from storing the storer that carries out the data array of planar alignment like this, utilization with the directions X of described data array as row, the scanning window of representing and can moving along described horizontal X and vertical Y with the size of row * X, read the described data array in this zone, and carry out computing according to these data, judge and have or not described cell, differentiate this cell size, respectively the number of described cell is counted by the cell size, method for cell count in the described analytical equipment is to adopt following method, it sets described scanning window is by judging whether all be the 1st window of " 0 " with the size of 1 * X1 (X1 is the constant of integer range) in this zone, be positioned at described the 1st window at the next line of described the 1st window and judge in this zone whether be the 2nd window of " 1 " with 1 * 1 size in the centre position of directions X, and each row of judging this zone with Y * X1 (Y is the variable of integer range) size in the next line position that is positioned at described the 2nd window whether minimum comprise the scanning window of the 3rd window composition of " 1 ", and differentiate described cell size with this scanning window.
As mentioned above, with scanning window scanning and reading of data array the time, since the 2nd window judge be expert at * whether be " 1 " in the zone of the size of X=1 * 1, therefore can not judge the data in same place, can not repeat to judge same data to the cell on the track, can differentiate the cell size, and respectively number be counted by size.
In addition, the method for cell count in the described analytical equipment in the present invention the 6th aspect is in the method for cell count in the described analytical equipment in the 5th aspect, and X1 is set at the value of the offset scope that causes greater than the error because of the sampling starting point.
As mentioned above, because the 1st window and the 3rd window carry out data judging with the X1 greater than the offset scope as X,, also can detect the position of " 1 " even therefore the sampling starting point is offset.
In addition, method for cell count in the described analytical equipment in the present invention the 7th aspect, be aspect the 5th or the described analytical equipment in the 6th aspect in method for cell count in, (Y2 and Y3 are integers if the cell that detects size is for Y2～Y3, the scope of Y2＜Y3), Y=Y2-1, utilize scanning window to begin the described data array in this zone is read, with the term harmonization of scanning window the time, successively Y is changed into Y2 in consistent location, Y2+1, carry out whether consistent judgement with the range of condition of described Y, up to become inconsistent in condition or Y=Y3 before, the described data array in this zone is read.
As mentioned above, the 3rd window does not need to read blank scope when scanning window switches, and can shorten and detect institute's time spent.
In addition, method for cell count in the described analytical equipment in the present invention the 8th aspect, be aspect the 5th in the method for cell count to the described analytical equipment of the 7th aspect either side, to the track irradiating laser on the disk for analyzing that injects cell, utilization is changed by the light quantity of light time by photodetector, judge have acellular.
As mentioned above, owing to only utilize laser radiation, by changed by the light quantity of light time, judge have on the track acellular, when therefore cell exists in orbit, only one " 1 " is deposited in storer, so the complicacy of the data processing can avoid there is a plurality of " 1 " time for a cell.
In addition, method for cell count in the described analytical equipment in the present invention the 9th aspect, the two-value numerical value of " 0 " or " 1 " that will obtain according to the cell of the multiple size of formation that has or not the analysis disc to inject carries out planar alignment with horizontal X and vertical Y, from storing the storer that carries out the data array of planar alignment like this, utilization with the directions X of described data array as row, the scanning window of representing and can moving along described horizontal X and vertical Y with the size of row * X, read the described data array in this zone, and carry out computing according to these data, judge and have or not described cell, differentiate this cell size, respectively the number of described cell is counted by the cell size, method for cell count in the described analytical equipment is to adopt following method, it sets described scanning window is by judging whether all be the 1st window of " 0 " with the size of 1 * X1 (X1 is the constant of integer) in this zone, be positioned at described the 1st window at the next line of described the 1st window and judge in this zone whether be the 2nd window of " 1 " with 1 * 1 size in the centre position of directions X, judge that with Y1 * X1 (Y1 is the variable of integer) size the capable whether minimum of each of this zone comprises the 3rd window of " 1 " in the next line position that is positioned at described the 2nd window, and judge that with the size of 1 * X1 (X1 is the variable of integer) whether this zone all is the scanning window that the 4th window of " 0 " is formed, and differentiate described cell size with this scanning window in the next line position that is positioned at described the 3rd window.
As mentioned above, with scanning window scanning and reading of data array the time, since the 2nd window judge be expert at * whether be " 1 " in the zone of the size of X=1 * 1, therefore can not judge the data in same place, can not repeat to judge same data to the cell on the track, only use the window run-down for a detected magnitude of obtaining, can differentiate the cell size, and respectively number be counted by size.
In addition, the method for cell count in the described analytical equipment in the present invention the 10th aspect is aspect the 9th in the method for cell count in the described analytical equipment, and X1 is set at the value of the offset scope that causes greater than the error because of the sampling starting point.
As mentioned above, because the 1st window and the 3rd window carry out data judging with the X1 greater than the offset scope as X,, also can detect the position of " 1 " even therefore the sampling starting point is offset.
In addition, method for cell count in the described analytical equipment in the present invention the 11st aspect, be aspect the 9th or the 10th in the method for cell count in the described analytical equipment, to the track irradiating laser on the disk for analyzing that injects cell, utilization is changed by the light quantity of light time by photodetector, judge have acellular.
As mentioned above, owing to only utilize laser radiation, by changed by the light quantity of light time, judge have on the track acellular, when therefore cell exists in orbit, only one " 1 " is deposited in storer, so the complicacy of the data processing can avoid there is a plurality of " 1 " time for a cell.
In addition, the described analytical equipment in the present invention the 12nd aspect, be at disk for analyzing irradiating and detecting light to the injection cell, and according to being subjected to photodetector to wash in a pan in the analytical equipment that data count described cell, the storer of first storage usefulness of data bus is given in the cell information analysis with binaryzation that every track on the described disk for analyzing is obtained, the window that can in described memory area, move, described window is moved the window mobile control unit of control, come the cell size decision unit of recognizing cells and decision size according to the arrangement of " 1 " in the described window, after the described cell recognition this counting added 1 cell count unit, and the storage rewriting unit that after described cell recognition, " 1 " is rewritten as " 0 ".
As mentioned above, differentiate and counting in order to carry out the cell size, because carrying out a window scanning on storer can finish, therefore whole cell recognition is all carried out under identical conditions, can improve counting precision and shorten minute,, therefore also not need to utilize again storer owing to do not need to change the window size that the bigger cell of identification is used, by once gathering and the storer interscan, just can carry out the cell size and differentiate and counting.
In addition, the described analytical equipment in the present invention the 13rd aspect, be aspect the 12nd in the described analytical equipment, in the window mobile control unit, has the window that in memory area, makes 1 * 1 size window scanning element along address direction scanning, in described window scanning, judge " 1 " identifying unit that has or not " 1 ", detect the cell size counter of " 1 " just this quantity being counted with described identifying unit at every turn, enlarge the window control module of window up to the place of " 1 " found with described identifying unit, detect " 1 " just makes the sweep interval displacement of " 1 " along the position direction shift unit with described identifying unit at every turn, and the region of search control module that makes the scope of window scanning in the sweep interval of restriction displacement.
As mentioned above, find that at first " 1 " is just big or small to the place that " 1 " is arranged along position direction extended window successively therefrom, by like this, can determine to a cell window size, be the cell size of orbital direction, owing to do not need to change the window size that the bigger cell of identification is used, therefore do not need to utilize again storer yet,, just can carry out the cell size and differentiate and counting by once gathering and the storer interscan.
In addition, the described analytical equipment in the present invention the 14th aspect is aspect the 12nd or the described analytical equipment in the 13rd aspect in, in region of search control module, extrapolate along the size of address direction diffusion, with its region of search according to desirable cell size as next bit.
As mentioned above, by only searching for the scope of appointment from " 1 " of initial discovery, can prevent that with other cell recognition be same cell, owing to do not need to change the window size that the bigger cell of identification is used, therefore do not need to utilize again storer yet, by once gathering and the storer interscan, just can carry out the cell size and differentiate and counting.
In addition, the scope of appointment can prevent that with other cell recognition be same cell, owing to do not need to change the window size that the bigger cell of identification is used, therefore do not need to utilize again storer yet,, just can carry out the cell size and differentiate and counting by once gathering and the storer interscan.
In addition, method for cell count in the described analytical equipment in the present invention the 15th aspect, be the method for cell count of the analytical equipment described cell counted to the disk for analyzing irradiating and detecting light that injects cell and according to the data that are subjected to light with photodetector, the method for employing is included in the window that makes 1 * 1 size in the memory area along address direction scanning and detect the operation 1 of " 1 "; With detected " 1 " is the operation 2 that the center expands to window the size of 1 * X6 (X6 is the constant of integer range); The window of described 1 * X6 is set if having " 1 " then to enlarge window at next bit up to described the next operation 3 at this window; Repeat described operation 2 and operation 3 processing, in window, do not detect the operation 4 of " 1 "; Do not finish then that described window enlarges and if the Y direction size of this window is the operation 5 that setting is then counted as cell if in described window, detect " 1 "; And the operation 6 that " 1 " in the described expansion window all is rewritten as " 0 " and begins repetition from the processing of described operation 1.
As mentioned above,, do not need repeatedly to redeterminate whether this cell is arranged, can obtain, short time and differentiate cell size accurately and counting by a secondary data for the cell on the track.
As mentioned above, according to the present invention, by optimizing the amount of movement of window, not repeating same bit in the feasible scanning scans, even the mixing with cells of different sizes exists, also can obtain and obtain desirable cell number by a secondary data, can analyze accurately with the short time.
In addition, by window size additional cell recognition result in the sample storer, can differentiate the size of cell, even the mixing with cells of different sizes exists for each scanning, also can obtain and obtain desirable cell number, can analyze accurately with the short time by a secondary data.
Have, freely change window size by one side in scanning, one side scans, can differentiate the size of cell, even the mixing with cells of different sizes exists, also can obtain and obtain desirable cell number by a secondary data, can analyze accurately with the short time.
In addition, 1 * 1 judgement window of the judgement window of the detection 0 that utilization is provided with at the 1st row of scanning window and the detection 1 that is provided with at the 2nd row of scanning window, cell on the track is detected reliably the starting position of data, even by not deleting the data in the window like this, can not repeat to judge same data yet, can differentiate cell size and count.
Therefore,, do not need repeatedly to redeterminate whether this cell is arranged for the cell on the track, can be with the short time and correctly differentiate cell size and count.
In addition,, therefore do not need to utilize again storer yet,, just can carry out the cell size and differentiate and counting by once gathering and the storer interscan owing to do not need to change the window size that the bigger cell of identification is used.
Therefore,, can count, therefore can improve counting precision and shorten minute a plurality of cells owing to the storer interscan of passing through once.In addition,, therefore not only can know the cell number of every kind of size, and the cell that can remove unwanted size comes display image etc. owing to can correctly judge the size of cell.
Description of drawings
Fig. 1 represents the key diagram of the analytical approach of analytical equipment in the past.
Fig. 2 represents to analyze in the past the analytical equipment key diagram of the method for the cell that varies in size.
Fig. 3 represents the block scheme of the analytical equipment of the invention process form 1.
Fig. 4 represents window scanning sequency figure of the present invention.
Fig. 5 represents the block scheme of the analytical equipment of the invention process form 2.
Fig. 6 represents the block scheme of the analytical equipment of the invention process form 2.
Fig. 7 represents the key diagram of the cell detection method in the method for cell count of the invention process form 4.
Fig. 8 represents the key diagram of the window scanning of this example 4.
Fig. 9 represents the key diagram of the cell detection method in the method for cell count of the invention process form 5.
Figure 10 represents the key diagram of the window scanning of this example 5.
Figure 11 represents the key diagram of the cell detection method in the past the method for cell count.
Figure 12 represents the block scheme of the analytical equipment of the invention process form 6.
Figure 13 represents that the cell size in the analytical equipment of this example 6 is differentiated and the synoptic diagram of counting mode.
Figure 14 represents that the cell size in the analytical equipment of this example 6 is differentiated and the synoptic diagram of counting mode.
Figure 15 represents that the cell size in the analytical equipment of this example 6 is differentiated and the synoptic diagram of counting mode.
Figure 16 represents to reach the synoptic diagram of the date storage method in the storer in the analytical equipment of the invention process form 6 in the past.
Figure 17 represents that the cell size in this routine in the past analytical equipment is differentiated and the synoptic diagram of counting mode.
Figure 18 represents that the cell size in this routine in the past analytical equipment is differentiated and the synoptic diagram of counting mode.
Figure 19 represents that the cell size in the analytical equipment of the invention process form 7 is differentiated and the synoptic diagram of counting mode.
Figure 20 represents that the cell size in the analytical equipment of this example 7 is differentiated and the synoptic diagram of counting mode.
Figure 21 represents that the cell size in the analytical equipment of this example 7 is differentiated and the synoptic diagram of counting mode.
Embodiment
Following one side is with reference to accompanying drawing, and one side specifies the analytical equipment of expression the invention process form and the method for cell count of analytical equipment.
(example 1)
The analytical equipment of example 1 at first is described with Fig. 3 and Fig. 4.
Fig. 3 represents the block scheme of the analytical equipment of the invention process form 1, and Fig. 4 represents window scanning sequency figure of the present invention.In Fig. 3, the 109th, translucent CD, the 110th, the optical pickup that the CD irradiates light is used, the 111st, the laser of optical pickup 110 irradiations, the 112nd, receive laser 111 that sees through disc 109 and the photodetector A that is transformed to electric signal, the 113rd, deviation prism, the 114th, accept CD 109 laser light reflected 111 and be transformed to the photodetector B of electric signal, the 115th, according to signal from photodetector A112 and photodetector B114, one dimension cell recognition unit with the one dimension recognizing cells, the 116th, according to from the information of window 107 and window 108 two-dimentional cell recognition unit with two-dimentional recognizing cells, the 118th, the window transfer length calculation section of the next amount of movement of calculation window 107 and window 108, the 119th, the window storer that the memory window amount of movement is used, the 117th, judge the identifying unit as a result that the result that judged by two-dimentional cell recognition unit 116 is whether correct, the 120th, the window mobile control unit that moves of control window 107 and window 108 according to the content of window storer 119.
For above such analytical equipment that constitutes, below its action of explanation and effect.
At first, not shown sample is injected on the CD 109.After the injection, CD 109 is with the certain speed rotation, and optical pickup 110 is all the time to CD 109 irradiating lasers 111 therebetween.The part of laser 111 sees through CD 109, accepts with photodetector A112.In addition, a part is by CD 109 reflections, and this reflected light is accepted with photodetector B114 with deviation prism 113 refractions.Open shown in the 2002-22651 as the Jap.P. spy, though photodetector A112 is more certain than being always with the signal of photodetector B114, but when having cell in sample, transmitted light is subjected to the clean of cell and changes, thereby the signal ratio of photodetector A112 and photodetector B114 changes.In one dimension cell recognition unit 115,, judge to have to be difficult the cell that one dimension is seen according to the variation of this signal ratio.Here, when being judged as cell, for the corresponding bit of each track of disc, put one ' 1 ' for a cell, put ' 0 ' in addition bit simultaneously, will be somebody's turn to do ' 1 ' ' 0 ' signal and deposit storer in certain sampling interval.
Then, the relevant two-dimentional cell recognition of narration.Here, suppose in same sample exist want the cell 105 that detects and 2 times of sizes thereof do not want the cell 106 (with reference to Fig. 2) that detects.
At first, in window transfer length calculation section 118, window moves each time, make that the window of scanning is not overlapping according to window size calculation window size calculation window amount of movement at that time, and the zone of not scanning.Current if establishing the size of window 107 is 3 row * 3 row, then for the identical cell of repeat count not, window 107 place of moving is the local now 3bit tangentially of distance, the place of direction along ng a path 3bit in addition next time.This amount of movement deposits window storer 119 in.Specifically, the tangent line of window storer 119 partly stores 3.Then, next tangentially during mobile 1bit, this value is subtracted 1, become 2 at window.Then, when mobile 3bit, just become 0.In addition, the corresponding location storage 3 of the tangent position with sample storer 101 of the amount of movement of orbital direction on window storer 119.This is also identical with the tangent line part, and when the window direction along ng a path moved 1bit, this value subtracted 1, became 2, when mobile 3bit, became 0.
Then, use 107 pairs of sample storeies of window of the same size, 101 enterprising line scannings with the cell of wanting to detect 105.Here, utilize the control of window mobile control unit 120, tangentially reach orbital direction respectively and carry out window scanning, here hypothesis as shown in Figure 4, sweep trace as televisor, at first scan along the end to end of switching direction from sample storer 101, direction along ng a path skew 1bit is undertaken by said sequence then.
Two dimension cell recognition unit 116 checks whether have ' 1 ' through some bit of the window 107 of being everlasting in window 107 tangential scannings, if when existing, then being judged as in two dimension has cell.Then, identifying unit 117 is with reference to the content of window storer 119 as a result, when being ' 0 ' with data in the corresponding window storer in the present place of window, when promptly being only limited to window and moving to recognizing cells once regional unduplicated regional, it is correct to be judged as the result who is judged as cell with two-dimentional recognition unit 116.
Here, also the window storer can be set at the white space of sample storer.
In addition, also the content of sample storer can be carried out reversible compression here, or deposit the sampling interval that the sample storer is used in, save the sample memory span by increasing.
Have, when amount of movement was 3bit, the scan mode of explanation was that one side makes bit skew of bit of window again, and the content of an acknowledgement window storer scans but also can whenever jump over 3bit quickly.
As mentioned above, in this example, deposit the window storer in owing to will represent the information that only moves with the corresponding distance of window size, move by such Optimal Control window, thereby can prevent the same cell of duplicate detection, do not need to change window size at every turn and all obtain data again, therefore can obtain and obtain desirable cell number, the analytical equipment of analyzing accurately with the short time can be provided by a secondary data.
(example 2)
The analytical equipment of example 2 is described with Fig. 5 below.
Fig. 5 represents the block scheme of the analytical equipment of the invention process form 2.In Fig. 5, the 122nd, to being judged as the data extra cell of the part additional data of cell with two-dimentional cell recognition unit 116, the 121st, differentiate the cell size judgement unit of cell size according to data extra cell 122 additional data.Be deletion window transfer length calculation section 118 and window storer 119, supplemental data extra cell 122 and cell size judgement unit 121 with the difference of the formation of example 1.
Below its action of explanation and effect.
At first, identical with example 1, carry out two-dimentional cell recognition.At this moment, with data extra cell 122 to the window of each scanning bit additional data to the sample storer 101 that is identified as cell.For example, when window 107 had been identified as cell, the left side additional data to ' 1 ' in the window 107 became ' 11 ', and when window 108 had been identified as cell, two left side additional datas to ' 1 ' in the window 108 became ' 101 '.In addition, in the method, when window 107 and 108 all has been identified as cell, become ' 111 '.In addition, utilize window mobile control unit 120 to confirm to have or not this data, thereby adjust the window amount of movement, the control window moves, and makes that the window of scanning is not overlapping, and the zone of not scanning.
Then, confirm data, and differentiate actual cell size based on this with each additional window size of data extra cell 122 with cell size judgement unit 121.For example, suppose that with the place that is identified as cell in window 108 scannings be the part that has been judged as cell with window 107.In this case, in esse cell is a cell with window 108 corresponding sizes.But, because earlier the window 107 of scanning is 1/2 a size of window 108, therefore ought to be with this cell recognition two with window 107 corresponding cells.For this reason, the numerical value with the counting of two cells of window 107 identification must subtract 2.
In example 1, be the amount of movement of control window, make not multiple scanning, and in example 2, by detecting cell with each window, thereby the sample storer is added specific data.By like this, can prevent multiple scanning, simultaneously the cell number of different sizes is counted.
In addition, also can be when swept memory at first, when even there is one ' 1 ' in the same tangent position of each track, deposit not shown cell compartment storer in ' 1 ', deposit not shown cell compartment storer in ' 0 ' in addition, control by utilizing storer to jump over control module like this, make and only scan the zone that cell exists, the sample memorizer information that saves the non-existent tangent position of visit cell when scanning again carries out the invalidation that two dimension is discerned.
As mentioned above, in this example,, can keep the resume which window has carried out two-dimentional cell recognition, thereby can prevent the same cell of duplicate detection by additional data.By like this, all obtain data again owing to do not need to change window size at every turn, therefore can obtain and obtain desirable cell number by a secondary data, the analytical equipment of analyzing accurately with the short time can be provided.
(example 3)
The analytical equipment of example 3 is described with Fig. 6 below.
Fig. 6 represents the block scheme of the analytical equipment of the invention process form 3.In Fig. 6, the 123rd, in scanning way, switch the window switch unit of the window that sample storer 101 is scanned.Be with the difference of the formation of example 2, append window switch unit 123, data extra cell 122 is replaced into data delete unit 124.
Below its action of explanation and effect.
At first, identical with example 1 and example 2, carry out two-dimentional cell recognition.Then, window 107 scans on a bit ground a bit on the sample storer 101 along cutting a direction.Then, when two-dimentional cell recognition unit, certain place 116 recognizing cells, window switch unit 123 switches to window 108 with window 107, and two-dimentional cell recognition unit 116 carries out cell recognition once more.At this moment, window 107 and window 108 two aspect can both recognizing cells the time, cell size judgement unit 121 judges that these cells are the cells with window 108 corresponding sizes.In addition, only using window 107 can recognizing cells the time, cell size judgement unit 121 judges that these cells are the cells with window 107 corresponding sizes.Then, identical with conventional art, data delete unit 124 is rewritten as ' 0 ' with whole ' 1 ' in the detection window, and this is the processing in order to prevent that the same cell of duplicate detection from now on from carrying out.In addition, the window of establishing scanning at first in this explanation is a window 107, but this promptly uses window 108 also passable.But, opposite with aforesaid processing in this case, must be in place that can not recognizing cells with two-dimentional cell recognition unit 116 switch window one by one.
As mentioned above, in this example, because in window scanning, switch window size before the data in rewriteeing the sample storage, scan again, know the cell number that goes out with windows detecting one by one, all do not obtain data again so do not need to change window size at every turn, therefore can obtain by a secondary data and obtain desirable cell number, the analytical equipment of analyzing accurately with the short time can be provided.
(example 4)
The following describes the method for cell count of the invention process form 4.
Fig. 7 represents the key diagram of the cell detection method in the method for cell count of this example 4, and Fig. 7 (a) is the key diagram of position cell and track and laser relation for the determination object object on the analysis disc in the method for cell count of this example 4.Fig. 7 (b) is for differentiating the key diagram of the big or small method of also counting respectively by size of cell with window in the method for cell count of this example 4.
In Fig. 7 (a), the 201st, analyzing the determination object object that injects on the disc is cell, the 202nd, analyze the track on the disc, the 203rd, analyzing the laser that relatively moves on the disc.The analytical equipment of this example 4 is to analyze disc injection sample, in the cell 201 of the various size of formation that in sample, exists, analyze the number of specific cells, in such analytical equipment, analyze on the disc identical with CD such as CD-ROM, be carved with spiral track 202, when analyzing the disc rotation, control, make laser 203 on track 202, relatively move.
On the other hand, the determination object object is the width of cell 201 greater than track 202, exists across many tracks 202, and whether when laser 203 is mobile on track 202, depending on has cell 201 on the track 202, produces signal in the laser light receiving unit and changes.By this signal transformation is handled, when being judged to be cell 201, " 1 " is deposited in storer 204, when situation in addition, " 0 " is deposited in storer 204, shown in Fig. 7 (b), store as data array.
In addition, about the scanning window in the method for cell count of this example 4, wherein go up directions X with the data array of storer 204 as row, window size is represented with row * X, shown in Fig. 7 (b), data array to storer 204, setting is by the 1st window 205A, the scanning window 205 that the 2nd window 205B and the 3rd window 205C form, the 1st window 205A judges whether all be " 0 " with the size of 1 * X1 (X1 is the constant of integer) in this zone, the 2nd window 205B is that the next line at the 1st window 205A is positioned at the 1st window 205A and judges in this zone whether be " 1 " in the centre position of directions X with 1 * 1 size, and whether minimum the 3rd window 205C judge each of this zone capable comprise " 1 " in the next line position that is positioned at the 2nd window 205B with Y * X1 (Y is the variable of integer) size.
The method that adopts is, if such scanning window 205 can move along horizontal X and vertical Y of data array, utilize this scanning window 205, from the sampling starting point is that the upper left corner of data array begins scanning, to skew one by one, if arrive the last of row, then with the left end of next line as beginning, window is moved, one side skew one by one successively from left to right again, the place of the term harmonization in a region retrieval and each window.
In addition, according to the cell size of obtaining, the size of vertical Y of window 205C is then different.Order when the expression detection occupies the cell of six track sizes as an example among Fig. 8.
At first, if the size of the window 205C in the scanning window 205 is 5 * X1 (X1=7 in Fig. 8 (a)), shown in Fig. 8 (a), window is scanned to the right, if arrive the last of this row, then with the left end of next line as beginning, window is moved, skew one by one successively from left to right again, the place that retrieval satisfies condition.
When finishing whole range of search, the testing result of utilizing scanning window 205 to obtain, expression occupies the number of 6 tracks and above cell.In the data array of Fig. 8, during with above-mentioned scanning window 205 retrieval, shown in Fig. 8 (b) like that, detect three places as the place that satisfies condition, then occupying 6 and above cell has three.
Then, the size of establishing window 205C is 6 * X1, and is same as described above, the skew one by one successively from a left side, the place that retrieval satisfies condition.Like this, when finishing whole range of search, the testing result of utilizing scanning window 205 to obtain, expression occupies the number of 7 tracks and above cell.In the data array of Fig. 8, shown in Fig. 8 (c) like that, detect two places as the place that satisfies condition, then occupying 7 and above cell has two.
According to the above, obtain existence across 6 tracks and above cell and there is number across 7 tracks and above cell, can obtain the number of the cell that occupies 6 track sizes according to their difference.By so as can be known, in the data array of Fig. 8, there is one in the cell that occupies 6 sizes.Here, establish X1 and be round values greater than the error range of data array.
At Fig. 8 (b) and beyond the detection position (c), owing to do not satisfy each window 205A, 205B of scanning window 205, the testing conditions of 205C, therefore do not need as in the past, the deleted data in order not repeat reading of data is as long as no longer carry out data determination.
(example 5)
The following describes the method for cell count of the invention process form 5.
Fig. 9 represents the key diagram of the cell detection method in the method for cell count of this example 5, and Fig. 9 (a) represents that the determination object object on the analysis disc in the method for cell count of this example 5 is the key diagram of the position relation of cell and track and laser.Fig. 9 (b) represents in the method for cell count of this example 5 to differentiate the cell size with window and the key diagram of the method counted respectively by size.
In Fig. 9 (a), the 201st, it is cell that the side of injecting on the analysis disc is decided target object, the 202nd, analyze the track on the disc, the 203rd, analyzing the laser that relatively moves on the disc.
In addition, in the method for cell count of this example 5, because the processing till storage data array in storer 204 is identical with example 4, therefore the explanation is here omitted.
About the scanning window in the method for cell count of this example 5, wherein go up directions X with the data array of storer 204 as row, window size is represented with row * X, shown in Fig. 9 (b), data array to storer 204, setting is by the 1st window 206A, the 2nd window 206B, the scanning window 206 that the 3rd window 206C and the 4th window 206D form, the 1st window 206A judges whether all be " 0 " with the size of 1 * X1 (X1 is the variable of integer) in this zone, the 2nd window 206B is that the next line at the 1st window 206A is positioned at the 1st window 206A and judges in this zone whether be " 1 " in the centre position of directions X with 1 * 1 size, whether minimum the 3rd window 206C judge each of this zone capable comprise " 1 " in the next line position that is positioned at the 2nd window 206B with Y1 * X1 (Y1 is the variable of integer) size, and the 4th window 206D judges with the size of 1 * X1 (X1 is the variable of integer) whether this zone all is " 0 " in the next line position that is positioned at the 3rd window 206C.
The method that adopts is, if such scanning window 206 can move along horizontal X and vertical Y of data array, utilize this scanning window 206, from the sampling starting point is that the upper left corner of data array begins scanning, to skew one by one, if arrive the last of row, then with the left end of next line as beginning, window is moved, one side skew one by one successively from left to right again, the place of the term harmonization in a region retrieval and each window.
In addition, according to the cell size of obtaining, the size of vertical Y of window 206C is then different.Order when the expression detection occupies the cell of six track sizes as an example among Figure 10.
At first, if the size of the window 206C in the scanning window 206 is 5 * X1 (X1=7 in Figure 10 (a)), shown in Figure 10 (a), window 206 is scanned to the right from the upper left corner of data array, if arrive the last of this row, then with the left end of next line as beginning, window is moved, skew one by one successively from left to right again, the place that retrieval satisfies condition.
When finishing whole range of search, the testing result of utilizing scanning window 206 to obtain, expression occupies the number of 6 tracks and above cell.In the data array of Figure 10, during with 206 retrievals of above-mentioned scanning window,, shown in Figure 10 (b), detect a place with scanning window 206 as the place that satisfies condition, the cell that then occupies six has one.
Except the detection position that utilizes scanning window 206 shown in Figure 10 (b), owing to satisfy the testing conditions of scanning container 06, therefore need be in order not repeat reading of data deleted data, therefore need be in order not repeat reading of data deleted data, as long as do not carry out data determination again.
Its result for the cell on the track, does not need repeatedly to redeterminate whether this cell is arranged, can be with the short time and correctly differentiate cell size and count.
(example 6)
The following describes the method for cell count of the invention process form 6.
Adopt and in the past the identical method of method to the method for memory area 313 store data inside as shown in Figure 16.
As shown in figure 13, at first establishing the window size that can move in memory area is 1 * 1, makes this window 301 scan (window scanning element) in memory area.As the window scan method in the window scanning element, be the 1st row the 1st row in the memory area, move along address direction successively, if the whole ends of scan in the zone of address direction then along one of position direction displacement, scan from the 1st leu again.Simultaneously utilize " 1 " identifying unit to judge and have or not " 1 ".One side is carried out this scanning, when the data by the zone are " 0 ", then keep former state and passes through, if find it is " 1 ", then temporarily stops there.At this moment, set in advance the cell size and used counter, every discovery is " 1 " once, and counter just adds 1.
As benchmark, the interval that makes search next " 1 " is along one (shift unit) of position direction displacement with " 1 " found here.Here, extrapolate along the size of address direction expansion, with its region of search (region of search control module) according to desirable cell size as next bit.In addition, along the size of the region of search of address direction expansion be fixed as with initial " 1 " as benchmark along address direction ± m the sample scope of (the m value depends on desirable cell size).But, excessive as if the m value is set at, then, therefore must stipulate and the corresponding m value of target cell size owing to might also be identified as same cell by attached other near cell.
Utilize the window scanning element in above such region of search that determines, 303 to scan, utilize " 1 " identifying unit to search for other " 1 "." if 1 ", then utilize the window size control module to make window expand as 302 and reach the place (Figure 14) that " 1 " is arranged, in the region of search of the next bit of this window, search for other " 1 " again.
Repeat these operations, in the region of search of next bit, be provided with the place of " 1 ", just finish the expansion of window.Be the function in the window mobile control unit so far.
Decide cell size (cell size decision unit) according to the big or small value of cell at that time, if the cell size of target is then counted Figure 15 by the cell count unit with it) with counter." 1 " in the window 304 of counting latter end utilizes the storage rewriting unit all to be rewritten as " 0 ", searches for " 1 " from the window 301 with 1 * 1 again.With this storage rewriting unit, can be according to purposes, only " 1 " with the cell data of specific size is rewritten as " 0 ", perhaps keeps same as before " 1 ".
If repeat these operations, then the scanning of the window in the memory area finishes usually, and the quantity of target cell is also counted.
In addition, in example 6, because when recognizing cells, its size also can determine, therefore also can count every kind of cell size, or only the cell of desired size be counted.
(example 7)
The method for cell count of the invention process forms 7 is described with Figure 19～21 below.
Adopt and in the past the identical method of method to the method for memory area 313 store data inside as shown in Figure 16.
As shown in figure 19, at first establishing the window size that can move in memory area is 1 * 1, and this window 305 is scanned in memory area.As the window scan method in the window scanning element, be the 1st row the 1st row in the memory area, move along address direction successively, if the whole ends of scan in the zone of address direction then along one of position direction displacement, scan from the 1st leu again.Simultaneously utilize " 1 " identifying unit to judge and have or not " 1 ".One side is carried out this scanning, when the data by the zone are " 0 ", then keep former state and passes through, if find it is " 1 ", then temporarily stops there.At this moment, set in advance the cell size and used counter, every discovery is " 1 " once, and counter just adds 1.
With find here ' 1 ' as benchmark, extrapolate along the size of address direction expansion according to desirable cell size, with window 305 these sizes of expansion, as position direction search window 306.In addition, be fixed as along the size of the region of search of address direction expansion that (the m value depends on desirable cell size along address direction ± m sample as benchmark with initial ' 1 ', then, therefore must stipulate and the corresponding m value of target cell size owing to might also be identified as same cell by attached other near cell.
Then, will search for the interval of the next one ' 1 ' along one of position direction displacement.If have ' 1 ' in the window after displacement,, continue this operation then again with one of window 306 downward single place shift.If do not have ' 1 ', then finish the displacement of window 306 along the position directions, along window so far 306 to pass through that part gathers be a window 307.That is, be in every each in the window 307 and comprise ' 1 ' state.
Position size according to window 307 decides the cell size, if the cell size of target is then counted it by the cell count unit." 1 " in the window 307 of counting latter end utilizes the storage rewriting unit all to be rewritten as " 0 ", searches for " 1 " from the window 305 with 1 * 1 again.With this storage rewriting unit, can be according to purposes, only " 1 " with the cell data of specific size is rewritten as " 0 ", perhaps keeps same as before " 1 ".
If repeat these operations, then the scanning of the window in the memory area finishes usually, and the quantity of target cell is also counted.
In addition, in example, because when recognizing cells, its size also can determine, therefore also can count every kind of cell size, or only the cell of desired size be counted.
Claims (15)
1. analytical equipment, inject on the disc contain the sample of cell and to this disc irradiates light after obtain the cell number of sample according to this reflection of light or transmitted light, it is characterized in that having
Carry out the one dimension cell recognition unit of cell recognition with one dimension according to the variation of described reflection of light light or transmitted light;
Storage is according to the recognition result of described one dimension cell recognition unit, is illustrated in the sample storer that the corresponding bit in each road with disc has acellular the 1st data to use;
To the window with any size on the described sample storer is that unit scans, by confirming described the 1st data, with the two-dimentional cell recognition unit of two-dimentional recognizing cells;
It is that unit is attached to the data extra cell in the described sample storer with each window that the expression that utilizes described two-dimentional cell recognition to discern is had acellular the 2nd data;
Utilize described the 2nd data to differentiate the cell size judgement unit of cell size; And
Control the window mobile control unit that described window moves,
By described each window of sample storer additional representation being had acellular the 2nd data, utilizes a secondary data to obtain size and the number thereof of obtaining cell.
2. analytical equipment, inject on the disc contain the sample of cell and to this disc irradiates light after obtain the cell number of sample according to this reflection of light or transmitted light, it is characterized in that having
According to the variation of described reflection of light light or transmitted light, carry out the one dimension cell recognition unit of cell recognition with one dimension;
Storage is illustrated in the sample storer that the corresponding bit in each road with disc has acellular the 1st data to use according to the recognition result of described one dimension cell recognition unit;
To the window with any size on the described sample storer is that unit scans, and comes the two-dimentional cell recognition unit with two-dimentional recognizing cells by confirming described the 1st data;
In the scanning of described test storer, switch the window switch unit of described window size arbitrarily;
The cell size judgement unit of the cell size that differentiation is discerned according to the scanning result that uses one or two and plural window size with described two-dimentional cell recognition unit; And
In the data delete unit of differentiating deletion described the 1st data in back with described cell size judgement unit,
When cell has been confirmed in the scanning that utilizes described sample storer, scan again and differentiate the cell size by changing window size, size and the number thereof utilizing a secondary data to obtain to obtain cell.
3. analytical equipment as claimed in claim 1 or 2 is characterized in that,
Make the employing cycle to change according to the size of the cell in the described sample.
4. analytical equipment as claimed in claim 1 is characterized in that,
The cell compartment storer at the interval that has when described sample storer being scanned, exists between torage cell and the cell with described window, and have when switching described window size and once more the sample storer is scanned, according to from described cell compartment canned data only the storer that scans of the zone that exists of pair cell jump over control module.
5. the method for cell count in the analytical equipment is characterized in that,
Described analytical equipment will carry out planar alignment with horizontal X and vertical Y according to the two-value data of " 0 " or " 1 " that has or not the cell of analyzing the multiple size of injecting on the disc of formation to obtain, from storing the storer that carries out the data array of planar alignment like this, utilization with the directions X of described data array as row, represent size and the scanning window that can move along described horizontal X and vertical Y with row * X, read the described data array in this zone, and carry out computing according to these data, judge and have or not described cell, differentiate this cell size, respectively the number of described cell is counted by the cell size;
Described method for cell count, setting described scanning window is by judging whether all be the 1st window of " 0 " with the size of 1 * X1 (X1 is the constant of integer range) in this zone, be positioned at described the 1st window at the next line of described the 1st window and judge in this zone whether be the 2nd window of " 1 " with 1 * 1 size in the centre position of directions X, and each row of judging this zone with Y * X1 (Y is the variable of integer range) size in the next line position that is positioned at described the 2nd window minimum scanning window that comprises the 3rd window composition of " 1 " whether, and differentiate described cell size with this scanning window.
6. the method for cell count in the analytical equipment as claimed in claim 5 is characterized in that,
X1 is set at the value of the offset scope that causes greater than the error because of the sampling starting point.
7. as the method for cell count in claim 5 or the 6 described analytical equipments, it is characterized in that,
(Y2 and Y3 are integers if the cell that detects size is for Y2～Y3, the scope of Y2＜Y3), Y=Y2-1, utilize scanning window to begin the described data array in this zone is read, with the term harmonization of scanning window the time, consistent location successively Y is changed into Y2, Y2+1 ..., carry out whether consistent judgement with the range of condition of described Y, up to become inconsistent in condition or Y=Y3 before, the described data array in this zone is read.
8. as the method for cell count in each described analytical equipment of claim 5 to 7, it is characterized in that,
To the track irradiating laser on the disk for analyzing that injects cell, utilize changed by the light quantity of light time by photodetector, judge have acellular.
9. the method for cell count in the analytical equipment is characterized in that,
The two-value numerical value of " 0 " or " 1 " that will obtain according to the cell of the multiple size of formation that has or not the analysis disc to inject carries out planar alignment with horizontal X and vertical Y, from storing the storer that carries out the data array of planar alignment like this, utilization with the directions X of described data array as row, the scanning window of representing and can moving along described horizontal X and vertical Y with the size of row * X, read the described data array in this zone, and carry out computing according to these data, judge and have or not described cell, differentiate this cell size, respectively the number of described cell is counted by the cell size
Method for cell count in the described analytical equipment, setting described scanning window is by judging whether all be the 1st window of " 0 " with the size of 1 * X1 (X1 is the variable of integer) in this zone, be positioned at described the 1st window at the next line of described the 1st window and judge in this zone whether be the 2nd window of " 1 " with 1 * 1 size in the centre position of directions X, judge that with Y1 * X1 (Y1 is the variable of integer) size the capable whether minimum of each of this zone comprises the 3rd window of " 1 " in the next line position that is positioned at described the 2nd window, and judge that with the size of 1 * X1 (X1 is the variable of integer) whether this zone all is the scanning window that the 4th window of " 0 " is formed, and differentiate described cell size with this scanning window in the next line position that is positioned at described the 3rd window.
10. the method for cell count in the analytical equipment as claimed in claim 9 is characterized in that,
X1 is greater than the value because of the offset scope that causes of error of sampling starting point.
11. the method for cell count as in claim 9 or the 10 described analytical equipments is characterized in that,
To the track irradiating laser on the disk for analyzing that injects cell, utilize changed by the light quantity of light time by photodetector, judge have acellular.
12. an analytical equipment is counted described cell to the disk for analyzing irradiating and detecting light of injection cell and according to be subjected to wash in a pan data with photodetector, it is characterized in that, comprises
The cell information distribution of the binaryzation that every track on the described disk for analyzing is obtained is given the storer of first storage usefulness of data bus, the window that can in described memory area, move, described window is moved the window mobile control unit of control, come the cell size decision unit of recognizing cells and decision size according to the arrangement of " 1 " in the described window, after the described cell recognition this counting added 1 cell count unit, and the storage rewriting unit that after described cell recognition, " 1 " is rewritten as " 0 ".
13. analytical equipment as claimed in claim 12 is characterized in that,
In the window mobile control unit, has the window that in memory area, makes 1 * 1 size window scanning element along address direction scanning, in described window scanning, judge " 1 " identifying unit that has or not " 1 ", detect the cell size counter of " 1 " just this quantity being counted with described identifying unit at every turn, enlarge the window control module of window up to the place of " 1 " found with described identifying unit, detect " 1 " just makes the sweep interval displacement of " 1 " along the position direction shift unit with described identifying unit at every turn, and the region of search control module that makes the scope of window scanning in the sweep interval of restriction displacement.
14. as claim 12 or 13 described analytical equipments, it is characterized in that,
In region of search control module, extrapolate along the size of address direction diffusion, with its region of search as next bit according to desirable cell size.
15. the method for cell count in the analytical equipment is characterized in that,
Be the method for cell count of the analytical equipment described cell counted to the disk for analyzing irradiating and detecting light that injects cell and according to the data that are subjected to light with photodetector, comprise
In memory area, make the operation 1 of the window of 1 * 1 size along address direction scanning and detection " 1 ";
With detected " 1 " is the operation 2 that the center expands to window the size of 1 * X6 (X6 is the constant of integer range);
The window of described 1 * X6 is set if having " 1 " then to enlarge window at next bit up to described the next operation 3 at this window;
Repeat described operation 2 and operation 3 processing, in window, do not detect the operation 4 of " 1 ";
Do not finish then that described window enlarges and if the Y direction size of this window is the operation 5 that setting is then counted as cell if in described window, detect " 1 "; And
The operation 6 that " 1 " in the described expansion window all is rewritten as " 0 " and begins repetition from the processing of described operation 1.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP080295/2003 | 2003-03-24 | ||
| JP2003080295A JP2004287939A (en) | 2003-03-24 | 2003-03-24 | Analysis device |
| JP116411/2003 | 2003-04-22 | ||
| JP365383/2003 | 2003-10-27 |
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| CN1761867A true CN1761867A (en) | 2006-04-19 |
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| CN 200480007270 Pending CN1761867A (en) | 2003-03-24 | 2004-03-22 | Analysis device and method for cell count in the analysis device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821599B (en) * | 2007-10-09 | 2011-10-19 | 诺维茨公司 | Automated cell density adjustment method for producing analysis plate |
| CN101382500B (en) * | 2007-09-04 | 2012-05-09 | 索尼株式会社 | Light irradiation method, light irradiation device and particle analysis device |
| CN113066121A (en) * | 2019-12-31 | 2021-07-02 | 深圳迈瑞生物医疗电子股份有限公司 | Image analysis system and method for identifying repeat cells |
-
2003
- 2003-03-24 JP JP2003080295A patent/JP2004287939A/en active Pending
-
2004
- 2004-03-22 CN CN 200480007270 patent/CN1761867A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101382500B (en) * | 2007-09-04 | 2012-05-09 | 索尼株式会社 | Light irradiation method, light irradiation device and particle analysis device |
| CN101821599B (en) * | 2007-10-09 | 2011-10-19 | 诺维茨公司 | Automated cell density adjustment method for producing analysis plate |
| CN113066121A (en) * | 2019-12-31 | 2021-07-02 | 深圳迈瑞生物医疗电子股份有限公司 | Image analysis system and method for identifying repeat cells |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004287939A (en) | 2004-10-14 |
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