CN1748784A - Process for producing ampeptide element - Google Patents

Abstract

The technological process of producing amino peptide essence includes sorting hog nail, washing, hydrolysis, drying, crushing, superfine grinding, lixiviation to separate and resin exchange to purify, and has high bioavailability.

Description

Process for producing ampeptide element

Technical field:

What the present invention relates to is pharmaceutical technology, particularly the ampeptide elemente production method.

The prior art situation:

Ampeptide elemente is the treatment idiopathic thrombocytopenic purpura of the development eighties, leukopenia, and aplastic anemia also can be used for psoriatic biochemical drug.

Domestic existing process for producing ampeptide element is to adopt high-temperature hydrolysis process fully at present, and the hydrolyzed product convection drying is pulverized, and is processed into finished product.This process simple coarse fails effective ingredient in the raw material is carried out purification, causes the finished product active constituent content low, and bioavailability is low, and total nitrogen is 13%, and amino nitrogen accounts for 2% of total nitrogen.

Summary of the invention

The objective of the invention is to improve, the process for producing ampeptide element that a kind of method is easy, improve the active constituent content of bioavailability, raising product is provided at the deficiency of prior art ampeptide elemente production technology.

The inventive method is in former technology: Unguis Sus domestica is selected cleaning---on the basis of hydrolysis---drying---pulverizing, adopts micronizing, lixiviate separation, resins exchange technical method to reach purification, improves bioavailability.

Process of the present invention is:

(1), Unguis Sus domestica is selected, is cleaned;

(2), hydrolysis;

(3), drying;

(4), pulverize;

(5), micronizing: above-mentioned medicated powder is pressed 1: 2-3 adds purified water, stirs, and last colloid barreling mill gets the superfine powder suspension;

(6), lixiviate separates:

A, with above-mentioned mixed liquor in 1: the ratio of 7-8 adds 5% sodium hydroxide, stirs at interval lixiviate 20-24 hour; Filter then;

B, residue is added 5% sodium hydroxide again carry out lixiviate, filtration;

C, merging filtrate precipitate;

D, drying; With above-mentioned precipitate dried under reduced pressure, must make with extra care the ampeptide elemente crude product;

(7), resins exchange:

A, with above-mentioned crude product water dissolution, transfer pH value 2.5, adsorb with styrene type cation exchange resin, control flow velocity 500～1000ML/h treats that effluent adds when the ninhydrin solution heated and boiled shows bluish violet to receive effluent, till effluent PH5-6;

B, again with the ammonia eluting, receive eluent, to eluent PH14, and eluent adds the ninhydrin solution heated and boiled and do not show bluish violet, and eluent adds 10% sodium hydroxide and be alkaline solution, adds 2 5% copper-baths tests and do not show till the purples;

C, merging effluent and eluent;

(8), concentrate: with above-mentioned amalgamation liquid in 90 ℃ of left and right sides evaporation and concentration, to the thick paste shape;

(9), drying: must make with extra care ampeptide elemente.

Though technology of the present invention has increased the processing technique step on the basis of former technology, the product effective ingredient polypeptide, the amino acid content that make through this technology improve, and bioavailability improves, and product quality has than much progress than former handicraft product.

Survey report is as follows:

Project	20040401	20040402	20040403
				Character	Faint yellow	Faint yellow	Faint yellow
Differentiate	Be positive reaction	Be positive reaction	Be positive reaction
				Microbial limit	Up to specification	Up to specification	Up to specification
Total nitrogen	26.72％	26.52％	26.94％
				Amino nitrogen	2.58％	2.53％	2.50％

Embodiment:

(1), Unguis Sus domestica is selected, is cleaned;

(2), hydrolysis;

(3), drying;

(4), pulverize;

(5), micronizing: above-mentioned medicated powder was added purified water by 1: 2, stir, last colloid barreling mill gets the superfine powder suspension;

(6), lixiviate separates:

A, above-mentioned mixed liquor is added 5% sodium hydroxide in 1: 8 ratio, stirred lixiviate 24 hours every one hour; Filter then;

B, residue is added 5% sodium hydroxide again carry out lixiviate, filtration;

C, merging filtrate precipitate;

D, drying with above-mentioned precipitate dried under reduced pressure, must be made with extra care the ampeptide elemente crude product;

(7), resins exchange:

A, with above-mentioned crude product water dissolution, transfer pH value 2.5, adsorb with styrene type cation exchange resin, control flow velocity 500～1000ML/h treats that effluent adds when the ninhydrin solution heated and boiled shows bluish violet to receive effluent, till effluent PH5-6;

B, again with the ammonia eluting, receive eluent, to eluent PH14, and eluent adds the ninhydrin solution heated and boiled and do not show bluish violet, and eluent adds 10% sodium hydroxide and be alkaline solution, adds 2 5% copper-baths tests and do not show till the purples;

C, merging effluent and eluent;

(8), concentrate: with above-mentioned amalgamation liquid in 90 ℃ of left and right sides evaporation and concentration, to the thick paste shape;

(9), drying:, must make with extra care ampeptide elemente with drying under reduced pressure case drying.

Claims (1)

1, process for producing ampeptide element is characterized in that:

(1), Unguis Sus domestica is selected, is cleaned;

(2), hydrolysis;

(3), drying;

(4), pulverize;

(5), micronizing: above-mentioned medicated powder is pressed 1: 2-3 adds purified water, stirs, and last colloid barreling mill gets the superfine powder suspension;

(6), lixiviate separates:

A, with above-mentioned mixed liquor in 1: the ratio of 7-8 adds 5% sodium hydroxide, stirs at interval lixiviate 20-24 hour; Filter then;

B, residue is added 5% sodium hydroxide again carry out lixiviate, filtration;

C, merging filtrate precipitate;

D, drying; With above-mentioned precipitate dried under reduced pressure, must make with extra care the ampeptide elemente crude product;

(7), resins exchange:

A, with above-mentioned crude product water dissolution, transfer pH value 2.5, adsorb with styrene type cation exchange resin, control flow velocity 500～1000ML/h treats that effluent adds when the ninhydrin solution heated and boiled shows bluish violet to receive effluent, till effluent PH5-6;

B, again with the ammonia eluting, receive eluent, to eluent PH14, and eluent adds the ninhydrin solution heated and boiled and do not show bluish violet, and eluent adds 10% sodium hydroxide and be alkaline solution, adds 2 5% copper-baths tests and do not show till the purples;

C, merging effluent and eluent;

(8), concentrate: with above-mentioned amalgamation liquid in 90 ℃ of left and right sides evaporation and concentration, to the thick paste shape;

(9), drying: must make with extra care ampeptide elemente.