CN1718918A - Method of removing resin in paper pulp by multienzyme synergistic action - Google Patents
Method of removing resin in paper pulp by multienzyme synergistic action Download PDFInfo
- Publication number
- CN1718918A CN1718918A CN 200510035390 CN200510035390A CN1718918A CN 1718918 A CN1718918 A CN 1718918A CN 200510035390 CN200510035390 CN 200510035390 CN 200510035390 A CN200510035390 A CN 200510035390A CN 1718918 A CN1718918 A CN 1718918A
- Authority
- CN
- China
- Prior art keywords
- paper pulp
- resin
- lipase
- lignin degradation
- degradation enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Paper (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention utilizes the combination action or composite action of lignin degradation enzyme and lipase to remove resin from paper pulp. Its resin removed rate is above 90%, and said method can obviously reduce COD pollution load in waste water.
Description
Technical field
The present invention relates to the method that a kind of multienzyme synergism effect removes resin in the paper pulp, specifically relate to a kind of collaborative i.e. associating that utilizes lignin degradation enzyme and lipase or compound action to remove the method for resin in mechanical pulp, sulfite pulp and the chemi-mechanical pulp.
Background technology
Resin is a kind of composition of paper making raw material, generally accounts for about 2～6% of timber dry weight.The resinae material is to be present in paper making raw material and to follow major pollutants in the whole process of production, difficult degradation is not easy to handle, and these pollutants enter in the waste water with the paper machine system, though making after the wastewater treatment can qualified discharge but can't further clean reuse, has wasted abundant water resource.The existence of resinae material causes pulp-making waste-water to be difficult to handle back cleaning reuse; Be present in the resinae material of copying in the paper system and can cause " pitch trouble ", promptly cause paper machine broken end, paper whiteness and intensity decreases and cause phenomenon such as the serious obstruction of machinery equipment.At present, paper mill is removed resin and is realized on a kind of electrolytic film, need to consume a large amount of electric energy, and this method is a kind of method that shifts the resin composition, the resin composition is not removed; Also having a kind of method is to add some dispersants such as kaolin, and the resinae material is disperseed, and it is agglomerating to be not easy to condense on paper or equipment surface.The existing removal methods of domestic paper mill not only will expend a large amount of electric energy, and is a kind of method that shifts resin, does not have degradative resin, and a large amount of resin compositions still can enter and copy in paper and the waste water system, makes waste water be difficult to reuse up to standard.
Proposed in recent years both at home and abroad with fungi comprise Ceratocystis fimbriata Strains (Ophiostoma, piliferum) and whiterot fungi remove wood chip or remove the patented technology of resin in the paper pulp as penetrating arteries and veins side bacterium (Phlebia radiate) with lipase, have certain degreasing effect.But there are long action time (the fungi effect needs more than 3 days), degreasing effect few (lipase acts on degreasing rate＜50% separately).Therefore, it is very necessary developing the deresinate new technology with independent intellectual property right.
Summary of the invention
The objective of the invention is to shortcoming, provide a kind of multienzyme synergism effect to remove the method for resin in the paper pulp at the prior art existence.Lignin degradation enzyme in this method has peroxidation to the resinae material, and the resin enzyme has hydrolysis to the resinae material.Compare with present defatting technology, have better degreasing effect, thereby eliminate paper industry " pitch trouble " problem that the resinae material causes, realize the pulping process cleaner production.
For achieving the above object, the present invention has taked following technical scheme:
The multienzyme synergism effect removes the method for resin in the paper pulp, it is characterized in that removing resin in the paper pulp for the synergy by lignin degradation enzyme and lipase or compound action.Described paper pulp is mechanical pulp, sulfite pulp or chemi-mechanical pulp.
The method that described multienzyme synergism effect removes resin in the paper pulp specifically comprises the steps: for the synergy by lignin degradation enzyme and lipase removes resin in the paper pulp
(1) the lignin degradation enzyme acts on paper pulp separately;
Mix with a kind of in the lignin degradation enzyme or by 1: 1 weight ratio wantonly two kinds or join in the paper pulp by three kinds of weight ratio mixing in 1: 1: 1, after paper pulp fully mixes, react 30～240min in the citric acid of 0.1～100mmol/L of pH2～6.5 or acetate buffer solution, reaction temperature is 18～50 ℃;
Total addition of lignin degradation enzyme is 0.001～5% of a paper pulp dry weight;
Described lignin degradation enzyme is respectively the lignin peroxidase of active 100～15000IU/ml, manganese peroxidase and the activity of active 100～15000IU/ml is the laccase of 100～15000IU/ml;
(2) after the lignin degradation enzyme reaction, add the lipase reaction that accounts for paper pulp dry weight 0.001～5% again, lipase active 10～20000IU/ml; Be reflected in the 0.025mol/L phosphate buffer of pH3～7.5 and carry out, action time 30～240min, room temperature.
The method that above-mentioned multienzyme synergism effect removes resin in the paper pulp also can remove the resin in the paper pulp for the compound action by lignin degradation enzyme and lipase, specifically comprises the steps:
Any and lipase in the lignin degradation enzyme are joined in the paper pulp lipase active 10～20000IU/ml simultaneously by 1: 1 weight ratio; Be reflected in the citric acid of 0.1～100mmol/L of pH2～6.5 or the acetate buffer solution and carry out, action time 30～240min, temperature is 28～45 ℃;
Described lignin degradation enzyme is respectively the lignin peroxidase of active 100～15000IU/ml, manganese peroxidase and the activity of active 100～15000IU/ml is the laccase of 100～15000IU/ml;
Total addition of lignin degradation enzyme is 0.001～5% of a paper pulp dry weight.
Lignin degradation enzyme described in the technique scheme is from whiterot fungi, and whiterot fungi produces consist of (g/L) of enzyme fluid nutrient medium: 0.1～1g K
2HPO
4, 1～10g KH
2PO
4, 0.24～2.4g MgSO
4, 0.1～1.0g CaCl
2, 0.01～0.1g NaCl, 0.1～1.0g FeSO
47H
2O
2, 0.01～0.1g ZnSO
47H
2O
2, 0.02～0.2gCuSO
45H
2O
2, 0.08～0.4g MnSO
4H
2O
2, 0.01～0.1gCoCl
2, 0.01～0.1g puridoxine hydrochloride and add 1～5% glucose and 0.1～1.0% ammonium tartrate as carbon source and nitrogenous source; During cultivation, pH2～6, temperature are 18～39 ℃;
Screening obtains the dote of whiterot fungi from forest through isolation and selection culture mediums (aperture＞60 purpose wood powder culture mediums), and further pass through ultraviolet radiation mutagenesis, by the hereditary and selection in 7～10 generations, the bacterial strain that filters out is the microorganism of public use.Use the domestication of previous crops adaptability.
Described lipase is fruit trichosporon cutaneum, penicillium expansum, geotrichum candidum or rhizopus, and trichosporon cutaneum, penicillium expansum, geotrichum candidum and rhizopus screen in the spontaneous bacterium colony from wood chips such as masson pine, and use Co
60The width of cloth is according to 1～30min or 40Wt ultraviolet ray irradiation 1～5min; The bacterial strain that does not produce blue metabolite that obtains behind 10～18 generation hereditary and selections is the microorganism of public use.Use the domestication of previous crops adaptability.
The fermentation condition of above-mentioned lipase can be following two kinds:
(1) full fat soybean 1～4%, MgSO
40.01 K～1%,
2HPO
4～1%, 0.1 (NH4)
2SO
40.2～2%, peanut oil 0.1～2%, the pH nature.
(2) dried silkworm chrysalis meal 1～10%, sucrose 0.5～2%, olive oil 0.1～1.5%, (NH
4)
2SO
40.1 MgSO～2%,
47H
2O 0.05-2%, K
2HPO
40.01～1%, the pH nature.
The best condition of enzyme production of described lipase is: initial pH6.5～9.5,30 ℃ of cultivation 12～60h, produce alkaline fat enzyme activity 10～20000IU/ml.
Compared with the prior art, the beneficial effect that the present invention has is: the resin in the paper pulp is carried out effective elimination, and the total removal efficiency of resin surpasses 90%, and pulp-making waste-water can clean reuse after the one-level biochemical treatment.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment:
Embodiment 1
Raw material: Masson Pine TMP slurry, TG mean value 1.86%.Lignin degradation enzyme (for the lignin peroxidase of active 153IU/ml) and lipase (active is 178IU/ml) are joined in the paper pulp by 1: 1 liquor ratio, join in the paper pulp, total addition is 0.01% (v/w) of paper pulp dry weight; After fully mixing with paper pulp, react 40min in the citric acid of the 0.2mmol/L of pH6.5 or acetate buffer solution, temperature is 39 ℃; After the ligninase reaction, add the lipase that accounts for paper pulp dry weight 0.01%, lipase active 178IU/ml again; Be reflected in the 0.25mol/L phosphate buffer of pH7.2 and carry out, action time 50min; Room temperature.
The result is as shown in table 1:
The paper pulp degreasing result of table 1. after embodiment 1 described condition is handled
| The enforcement time | TG content (%) before enzyme is handled | TG content (%) before enzyme is handled | Resin removal efficiency (%) |
| 2004.09.10 2004.11.12 2004.12.14 2005.01.25 | 1.86 1.88 1.83 1.85 | 0.14 0.12 0.15 0.11 | 92.5 93.6 91.8 94.1 |
Annotate: TG content is the content of resinous substances in the paper pulp.
Embodiment 2
Raw material: Masson Pine TMP slurry, TG mean value 1.86%.Lignin degradation enzyme (for the lignin peroxidase of active 267IU/ml) and lipase (active is 303IU/ml) join in the paper pulp by 1: 1 liquor ratio, and total addition is 0.03% (v/w) of paper pulp dry weight; Be reflected in the 0.3mmol/L citrate buffer solution of pH6.5 and carry out, action time 50min, temperature is 35 ℃.
The result is as shown in table 2:
The paper pulp degreasing result of table 1. after embodiment 1 described condition is handled
| The enforcement time | TG content (%) before enzyme is handled | TG content (%) before enzyme is handled | Resin removal efficiency (%) |
| 2004.09.11 2004.11.13 2004.12.15 2005.01.26 | 1.85 1.87 1.89 1.86 | 0.13 0.09 0.11 0.08 | 93.0 95.2 94.2 95.7 |
Annotate: TG content is the content of resinous substances in the paper pulp.
Embodiment 3
Raw material: masson pine sulfonation machine slurry, TG mean value 1.93%.Lignin degradation enzyme (for the lignin peroxidase of active 219IU/ml) and lipase (active is 248IU/ml) join in the paper pulp by 1: 1 liquor ratio, and total addition is 0.03% (v/w) of paper pulp dry weight; Be reflected in the 0.15mmol/L citrate buffer solution of pH 6.5 and carry out, action time 55min, temperature is 36 ℃.
The result is as shown in table 2:
The paper pulp degreasing result of table 1. after embodiment 1 described condition is handled
| The enforcement time | TG content (%) before enzyme is handled | TG content (%) before enzyme is handled | Resin removal efficiency (%) |
| 2004.09.11 2004.11.13 2004.12.15 2005.01.26 | 1.93 1.92 1.96 1.94 | 0.12 0.17 0.13 0.15 | 93.8 91.2 93.4 92.3 |
Annotate: TG content is the content of resinous substances in the paper pulp.
Claims (9)
1, a kind of multienzyme synergism effect removes the method for resin in the paper pulp, it is characterized in that removing resin in the paper pulp for the synergy by lignin degradation enzyme and lipase or compound action.
2, multienzyme synergism effect according to claim 1 removes the method for resin in the paper pulp, it is characterized in that described paper pulp is mechanical pulp, sulfite pulp or chemi-mechanical pulp.
3, multienzyme synergism effect according to claim 1 removes the method for resin in the paper pulp, it is characterized in that specifically comprising the steps: for the synergy by lignin degradation enzyme and lipase removes resin in the paper pulp
(1) the lignin degradation enzyme acts on paper pulp separately;
Mix with a kind of in the lignin degradation enzyme or by 1: 1 weight ratio wantonly two kinds or join in the paper pulp by three kinds of weight ratio mixing in 1: 1: 1, after paper pulp fully mixes, react 30～240min in the citric acid of 0.1～100mmol/L of pH 2～6.5 or acetate buffer solution, reaction temperature is 18～50 ℃;
Total addition of lignin degradation enzyme is 0.001～5% of a paper pulp dry weight;
Described lignin degradation enzyme is respectively the lignin peroxidase of active 100～15000IU/ml, manganese peroxidase and the activity of active 100～15000IU/ml is the laccase of 100～15000IU/ml;
(2) after the lignin degradation enzyme reaction, add the lipase reaction that accounts for paper pulp dry weight 0.001～5% again, lipase active＞100IU/ml; Be reflected in 0.025～2.5mol/L phosphate buffer of pH3～7.5 and carry out, action time 30～240min, room temperature condition.
4, multienzyme synergism effect according to claim 1 removes the method for resin in the paper pulp, it is characterized in that specifically comprising the steps: for the compound action by lignin degradation enzyme and lipase removes resin in the paper pulp
Any and lipase in the lignin degradation enzyme are joined in the paper pulp lipase active 10～20000IU/ml simultaneously by 1: 1 weight ratio; Be reflected in the citric acid of 0.1～100mmol/L of pH2～6.5 or the acetate buffer solution and carry out, action time 30～240min, temperature is 28～45 ℃;
Described lignin degradation enzyme is respectively the lignin peroxidase of active 100～15000IU/ml, manganese peroxidase and the activity of active 100～15000IU/ml is the laccase of 100～15000IU/ml;
Total addition of lignin degradation enzyme is 0.001～5% of a paper pulp dry weight.
5, remove the method for resin in the paper pulp according to claim 3 or 4 described multienzyme synergism effects, it is characterized in that described lignin degradation enzyme from whiterot fungi, what whiterot fungi produced the enzyme fluid nutrient medium consists of (g/L): 0.1～1gK
2HPO
4, 1～10g KH
2PO
4, 0.24～2.4g MgSO
4, 0.1～1.0g CaCl
2, 0.01～0.1g NaCl, 0.1～1.0g FeSO
47H
2O
2, 0.01～0.1g ZnSO
47H
2O
2, 0.02～0.2g CuSO
45H
2O
2, 0.08～0.4g MnSO
4H
2O
2, 0.01～0.1g CoCl
2, 0.01～0.1g puridoxine hydrochloride and add 1～5% glucose and 0.1～1.0% ammonium tartrate as carbon source and nitrogenous source;
During cultivation, pH2～6, temperature are 18～39 ℃.
6, remove the method for resin in the paper pulp according to claim 3 or 4 described multienzyme synergism effects, it is characterized in that described lipase is fruit trichosporon cutaneum, penicillium expansum, geotrichum candidum or rhizopus.
7, multienzyme synergism effect according to claim 6 removes the method for resin in the paper pulp, it is characterized in that the fermentation condition of described lipase is:
Full fat soybean 1～4%, MgSO
40.01 K～1%,
2HPO
4～1%, 0.1 (NH4)
2SO
40.2～2%, peanut oil 0.1～2%, pH nature.
8, multienzyme synergism effect according to claim 6 removes the method for resin in the paper pulp, it is characterized in that the fermentation condition of described lipase is:
Dried silkworm chrysalis meal 1～10%, sucrose 0.5～2%, olive oil 0.1～1.5%, (NH
4)
2SO
40.1 MgSO～2%,
47H
2O 0.05-2%,, K
2HPO
40.01～1%.
9, multienzyme synergism effect according to claim 6 removes the method for resin in the paper pulp, it is characterized in that the condition of enzyme production of described lipase is: initial pH cultivates 12～60h for 6.5～9.5,30 ℃, produces alkaline fat enzyme activity 10～20000IU/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510035390 CN1718918B (en) | 2005-06-24 | 2005-06-24 | Method of removing resin in paper pulp by multienzyme synergistic action |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510035390 CN1718918B (en) | 2005-06-24 | 2005-06-24 | Method of removing resin in paper pulp by multienzyme synergistic action |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1718918A true CN1718918A (en) | 2006-01-11 |
| CN1718918B CN1718918B (en) | 2010-04-14 |
Family
ID=35930854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510035390 Expired - Fee Related CN1718918B (en) | 2005-06-24 | 2005-06-24 | Method of removing resin in paper pulp by multienzyme synergistic action |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1718918B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174504A (en) * | 2011-01-14 | 2011-09-07 | 华南理工大学 | Granular carrier immobilized lipase for treating papermaking white water and preparation method thereof |
| CN102174505A (en) * | 2011-01-14 | 2011-09-07 | 华南理工大学 | Preparation method of granular carrier immobilized bienzyme for treating papermaking white water |
| CN103061184A (en) * | 2012-12-26 | 2013-04-24 | 泰兴市一鸣生物制品有限公司 | Method of controlling secondary gummy substances by using lipase |
| CN103074795A (en) * | 2012-12-26 | 2013-05-01 | 泰兴市一鸣生物制品有限公司 | Method for controlling secondary stickies through lipase |
| CN103289982A (en) * | 2012-03-01 | 2013-09-11 | 烟台大学 | Preparation of novel immobile zymophore |
| CN106223090A (en) * | 2016-07-29 | 2016-12-14 | 联盛纸业（龙海）有限公司 | A kind of wood chip defibrator process slurry substitutes OCC and joins the production method copying high-grade single spreading coated duplex board with grey back |
| CN106223091A (en) * | 2016-07-29 | 2016-12-14 | 联盛纸业（龙海）有限公司 | A kind of brush wood wood chip defibrator process slurry substitutes OCC slurry and joins the production method copying high-grade cardboard |
-
2005
- 2005-06-24 CN CN 200510035390 patent/CN1718918B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174504A (en) * | 2011-01-14 | 2011-09-07 | 华南理工大学 | Granular carrier immobilized lipase for treating papermaking white water and preparation method thereof |
| CN102174505A (en) * | 2011-01-14 | 2011-09-07 | 华南理工大学 | Preparation method of granular carrier immobilized bienzyme for treating papermaking white water |
| CN103289982A (en) * | 2012-03-01 | 2013-09-11 | 烟台大学 | Preparation of novel immobile zymophore |
| CN103289982B (en) * | 2012-03-01 | 2015-09-30 | 烟台大学 | A kind of preparation of novel fixed enzyme vector |
| CN103061184A (en) * | 2012-12-26 | 2013-04-24 | 泰兴市一鸣生物制品有限公司 | Method of controlling secondary gummy substances by using lipase |
| CN103074795A (en) * | 2012-12-26 | 2013-05-01 | 泰兴市一鸣生物制品有限公司 | Method for controlling secondary stickies through lipase |
| CN103074795B (en) * | 2012-12-26 | 2015-04-29 | 泰兴市一鸣生物制品有限公司 | Method for controlling secondary stickies through lipase |
| CN106223090A (en) * | 2016-07-29 | 2016-12-14 | 联盛纸业（龙海）有限公司 | A kind of wood chip defibrator process slurry substitutes OCC and joins the production method copying high-grade single spreading coated duplex board with grey back |
| CN106223091A (en) * | 2016-07-29 | 2016-12-14 | 联盛纸业（龙海）有限公司 | A kind of brush wood wood chip defibrator process slurry substitutes OCC slurry and joins the production method copying high-grade cardboard |
| CN106223091B (en) * | 2016-07-29 | 2017-12-15 | 联盛纸业（龙海）有限公司 | A kind of brush wood wood chip defibrator process slurry substitutes OCC slurries with the production method for copying high-grade cardboard |
| CN106223090B (en) * | 2016-07-29 | 2017-12-15 | 联盛纸业（龙海）有限公司 | A kind of wood chip defibrator process slurry substitutes OCC and matches somebody with somebody the production method for copying high-grade one side coating coated duplex board with grey back |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1718918B (en) | 2010-04-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1718918B (en) | Method of removing resin in paper pulp by multienzyme synergistic action | |
| Chen et al. | Biological pretreatment of lignocellulosics: potential, progress and challenges | |
| Clausen | Bacterial associations with decaying wood: a review | |
| Hakala et al. | Evaluation of novel wood-rotting polypores and corticioid fungi for the decay and biopulping of Norway spruce (Picea abies) wood | |
| Farrell et al. | Solving pitch problems in pulp and paper processes by the use of enzymes or fungi | |
| CN102634856A (en) | Method for preparing natural bamboo fibers in pectin removal manner by aid of compound microorganism bactericide | |
| CN108813161B (en) | Pretreatment method of straw biological feed raw material | |
| EP0584176A1 (en) | Process, using enhanced-action laccase enzymes, for the delignification or bleaching of lignocellulose-containing material or for the treatment of waste water | |
| Wuyep et al. | Production and regulation of lignin degrading enzymes from Lentinus squarrosulus (mont.) Singer and Psathyrella atroumbonata Pegler | |
| CN1421402A (en) | Microbial prepn for treating paper-making effluent and microbe culturing method | |
| Adaskaveg et al. | Effects of incubation time and temperature on in vitro selective delignification of silver leaf oak by Ganoderma colossum | |
| Yadav et al. | Ligninolytic microbes and their role in effluent management of pulp and paper industry | |
| CN103087966A (en) | Efficient degrading microbial inoculum for regenerated papermaking wastewater and preparation method thereof | |
| CN103642712B (en) | A kind of composite flora for paper waste of degrading and preparation method thereof | |
| Kannan et al. | Enzymology of ligno-cellulose degradation by Pleurotus sajor-caju during growth on paper-mill sludge | |
| EP1880053B1 (en) | Chlorine dioxide treatment compositions and processes | |
| CN1928074A (en) | Microorganism bacterium agent for degrading lignocellulose and method of degrading lignocellulose using the same | |
| Singh et al. | White and brown rot fungi as decomposers of lignocellulosic materials and their role in waste and pollution control | |
| Rameshaiah et al. | Applications of ligninolytic enzymes from a white-rot fungus Trametes versicolor. | |
| Sakdaronnarong et al. | Improving enzymatic saccharification of sugarcane bagasse by biological/physico-chemical pretreatment using Trametes versicolor and Bacillus sp. | |
| CN1115437C (en) | Method for all chlorine free bleaching paper pulp by biological technology | |
| Arora | Biodelignification of wheat straw by different fungal associations | |
| Shintani et al. | Decolorization of kraft pulp bleaching effluent by a newly isolated fungus, Geotrichum candidum Dec 1 | |
| Bogale | Biotechnological applications of white rot fungi: a review | |
| US5431820A (en) | Method for treating liquid waste after pulp bleaching |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100414 Termination date: 20120624 |