CN1707247A - Method for measuring content of aloe-polysaccharose - Google Patents
Method for measuring content of aloe-polysaccharose Download PDFInfo
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- CN1707247A CN1707247A CN 200410048001 CN200410048001A CN1707247A CN 1707247 A CN1707247 A CN 1707247A CN 200410048001 CN200410048001 CN 200410048001 CN 200410048001 A CN200410048001 A CN 200410048001A CN 1707247 A CN1707247 A CN 1707247A
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- aloe
- aloe polysaccharide
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- new method
- mensuration
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Abstract
The present invention provides one new method of determining aloe polysaccharide content. Theoretically, the present invention is based on visual light spectrophotometric process. Under alkali condition, aloe polysaccharide solution, one kind of developer and one kind of color assistant react to produce one kind of stable ternary color compound. By referring idle reagent, the absorbance of the solution is measured in a spectrophotometric process. The determining method has high selectivity, good linear relativity of work curves, wide application range and other advantages, and may be used in measuring aloe polysaccharide content in fresh aloe leaf and aloe product.
Description
Technical field
The present invention relates to the assay method of aloe product.
Background technology
Aloe is the Liliaceae per nnial herb, is that raw material is extensive day by day in the application of industries such as cosmetics, food, beverage and health products with bright leaf and aloe products in recent years.Aloe polysaccharide is the main biological activity of aloe, and its content is one of important physical and chemical inspection index of aloe product.The mensuration of aloe polysaccharide, what domestic and international application was maximum is Fehling reagent method and Dubois test method(s).These methods all are according to aloe polysaccharide is converted into monose, and then the principle of mensuration contents of monosaccharides, thereby monose such as compound sugar such as some the common adulterants in the middle of the aloe product such as polysaccharide, sucrose such as maltodextrin and glucose can not effectively distinguish, and the mensuration of aloe polysaccharide has been produced interference.The visible spectrophotometry of aloe polysaccharide and Congo red colour developing also has report, the selectivity of this method is better, monose such as compound sugar such as polysaccharide such as maltodextrin, sucrose and glucose to measure disturb less, but require the aloe polysaccharide content of test product can not be too high, can not be low excessively.When polyoses content is higher and since polysaccharide show acid can neutralization part alkali, thereby the pH value of solution is reduced greatly, drop to a certain degree, Congo redly will show black even become turbid; When polyoses content was low, the sensitivity of method was lower, error at measurment is bigger.So the application of this method also has been subjected to very big restriction.
Summary of the invention
The objective of the invention is to find the new method of the mensuration aloe polysaccharide content that a kind of selectivity is good, measurement range is wider, thereby measure the content of aloe polysaccharide in the aloe product.
Purpose of the present invention can be realized by following proposal:
1. the preparation of sodium hydroxide solution
Weighing sodium hydroxide 0.8g is in the 100mL volumetric flask, and thin up shakes up to scale.
2. the preparation of chromogenic reagent solution
Take by weighing Congo red 7mg in the 50mL volumetric flask, thin up shakes up to scale.
3. help the preparation of toner solution
Draw 100g/L human immunoglobulin 1.2mL in the 100mL volumetric flask, thin up shakes up to scale.
4. aloe polysaccharide Determination on content
Get the 1mL chromogenic reagent solution in test tube, add 5mL sodium hydroxide solution and 1mL and help toner solution, add the sample solution that 3mL contains aloe polysaccharide again, shake up.After 5 minutes, reagent blank is made reference, the 1cm cuvette, and the 525nm place measures solution absorbency.
Realize further describing of the object of the invention:
We's ratio juris is to utilize aloe polysaccharide and developer is Congo red and help the toner human immunoglobulin to generate a kind of stable ternary colored compound under strong alkaline condition, thereby reaches the purpose of mensuration aloe polysaccharide content.Aloe polysaccharide and developer are Congo red and help toner human immunoglobulin's effect more single-minded, under determined condition, 5 times of the quantity of common adulterant beta-schardinger dextrin-, starch, sucrose and the glucose etc. of aloe product still do not have influence to this method when aloe polysaccharide; The maltodextrin of equivalent does not have influence yet, and the maltodextrin of 5 times of amounts only has less influence.By the new method of measuring aloe polysaccharide content, measure polyoses content in 100: 1 aloe gel goods with calibration curve method, 5 groups of replicate determinations and carry out recovery analysis.The measurement result relative standard deviation is less than 5%, the recovery 90～96%.This shows that the new method of measuring aloe polysaccharide content has advantages such as instrument is simple, method of operating is easily grasped, analysis speed is fast, measurement result is accurate.That this method is applicable to is easy, fast, optionally measure the content of aloe polysaccharide in Fresh leaf of aloe and the aloe products, the detection that is particularly useful for palming off aloe products He mingles aloe products.Remedy the deficiency of aloe polysaccharide analytical approach in the existing aloe products, had very high practical value.
Claims (5)
1. the new method of measuring aloe polysaccharide is characterized in that control, the developer of pH value and helps the selection and the determination step of toner.
2. the new method of mensuration aloe polysaccharide according to claim 1, its feature are that also the consumption of sodium hydroxide solution is 5mL (concentration is 0.2M) with the pH value of NaOH control solution.
3. the new method of mensuration aloe polysaccharide according to claim 1, its feature are that also used developer is Congo red, and the consumption of developer is that (concentration is 2.0 * 10 to 1mL
-4M).
4. the new method of mensuration aloe polysaccharide according to claim 1, its feature are that also the used toner that helps is the human immunoglobulin, and the consumption that helps toner is 1mL (concentration is 1.2g/L).
5. the new method of mensuration aloe polysaccharide according to claim 1, its feature is that also determination step is: get the 1mL chromogenic reagent solution in test tube, add 5mL sodium hydroxide solution and 1mL and help toner solution, add the sample solution that 3mL contains aloe polysaccharide again, shake up.After 5 minutes, reagent blank is made reference, the 1cm cuvette, and the 525nm place measures solution absorbency.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410048001 CN1707247A (en) | 2004-06-11 | 2004-06-11 | Method for measuring content of aloe-polysaccharose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410048001 CN1707247A (en) | 2004-06-11 | 2004-06-11 | Method for measuring content of aloe-polysaccharose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1707247A true CN1707247A (en) | 2005-12-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410048001 Pending CN1707247A (en) | 2004-06-11 | 2004-06-11 | Method for measuring content of aloe-polysaccharose |
Country Status (1)
| Country | Link |
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| CN (1) | CN1707247A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316481A (en) * | 2014-11-12 | 2015-01-28 | 福州大学 | Determination method for grain beta-glucan content by Congo red method |
| CN108107010A (en) * | 2017-12-21 | 2018-06-01 | 无限极（中国）有限公司 | A kind of method for detecting lentinan content |
| CN112147092A (en) * | 2020-05-29 | 2020-12-29 | 江西师范大学 | Method for detecting acidic polysaccharide in water body |
| CN118067647A (en) * | 2024-04-17 | 2024-05-24 | 甘肃华隆芯材料科技有限公司 | Method for detecting hexafluoroacetone trihydrate content |
-
2004
- 2004-06-11 CN CN 200410048001 patent/CN1707247A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316481A (en) * | 2014-11-12 | 2015-01-28 | 福州大学 | Determination method for grain beta-glucan content by Congo red method |
| CN108107010A (en) * | 2017-12-21 | 2018-06-01 | 无限极（中国）有限公司 | A kind of method for detecting lentinan content |
| CN112147092A (en) * | 2020-05-29 | 2020-12-29 | 江西师范大学 | Method for detecting acidic polysaccharide in water body |
| CN118067647A (en) * | 2024-04-17 | 2024-05-24 | 甘肃华隆芯材料科技有限公司 | Method for detecting hexafluoroacetone trihydrate content |
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