CN1699358A - Method for extracting theaflavin - Google Patents
Method for extracting theaflavin Download PDFInfo
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- CN1699358A CN1699358A CN 200510075693 CN200510075693A CN1699358A CN 1699358 A CN1699358 A CN 1699358A CN 200510075693 CN200510075693 CN 200510075693 CN 200510075693 A CN200510075693 A CN 200510075693A CN 1699358 A CN1699358 A CN 1699358A
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- theoflavin
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Abstract
The invention discloses a process for extracting theaflavine, which comprises kneading fresh tea leaves, watering and grinding plasm, then carrying out fermentation and oxidation so as to obtain solution containing theaflavine, obtaining centrifugate through centrifugation deslagging, ultrafiltrating the centrifugate with ceramic film, finally processing the obtained filtrate through solvent extraction or membrane separating process.
Description
Technical field
The present invention relates to a kind of method of from tealeaves, extracting theoflavin.
Background technology
Theoflavin by theoflavin, theoflavin-3-gallic acid ester, theoflavin-3 '-gallic acid ester, theoflavin-3.3 '-four monomer components of gallic acid ester form, because it has anti-oxidant, antibiotic, deodorizing, removing free radical, anti-ageing, functions such as treatment cardiovascular disorder, be widely used in every field such as medicine, healthcare products, foodstuff additive, the method for extracting theoflavin at present mainly contains solvent extraction partition method, chemical reagent oxidizes method, polyphenol oxidase enzyme process.The solvent extraction partition method mainly is to carry out alcohol with ethanol to analyse separation, and resulting theoflavin content is lower, is not suitable for medicine and healthcare products and uses.The chemical reagent oxidizes method is by the control reaction conditions, with chemical reagent tea-polyphenol is transformed, though the theoflavin product yield height that obtains, chemical reagent can't reclaim.The polyphenol oxidase enzyme process is to utilize polyphenoloxidase to carry out oxidizing reaction under corresponding conditions, and the production process cost is higher, and the finished product content of gained is lower.
Summary of the invention
The extracting method that the purpose of this invention is to provide a kind of theoflavin.
Designed scheme of the present invention is: Fresh Folium Camelliae sinensis is added the water mill slurry after kneading, the oxidation of fermenting then obtains containing the solution of theoflavin, obtain centrifugate by centrifugal slagging-off, centrifugate gained filtrate behind ceramic membrane ultrafitration adopts solvent extration or membrane separation process to handle; Described solvent extration is that filtrate is used organic solvent extraction, and wherein solvent phase distills the theoflavin concentrated solution that obtains and changes molten with pure water again, the spray-dried theoflavin that obtains with citric acid solution wash-out caffeine.Described membrane separation process is that filtrate is separated with nanofiltration membrane, holds back the concentrated solution that obtains and handles with extraction process or resin adsorption method; Described extraction process is with the concentrated solution organic solvent extraction, and wherein solvent phase distills the theoflavin concentrated solution that obtains and changes molten with pure water again with citric acid solution wash-out caffeine, and spray-dried or lyophilize obtains theoflavin; Described resin adsorption method is that concentrated solution is diluted, with macroporous adsorbent resin theoflavin is adsorbed, with ethanol or propyl alcohol or the mixture wash-out of the two, Fractional Collections, distillating recovering solvent recycles, and theoflavin concentrated solution spraying drying or lyophilize are obtained theoflavin.Described organic solvent is an ethyl acetate.
The extracting method technology of a kind of theoflavin provided by the present invention is simple, and cost is low, meets environmental protection requirement; product yield height; the content height, and can satisfy the needs of industries such as medicine, healthcare products, food by the different different series product of processing condition output content of control.
Description of drawings
Fig. 1 is the process flow sheet of method for extracting theaflavin of the present invention.
Embodiment
Below the present invention is described in more detail.
The extracting method of the theoflavin that the present invention is designed comprises fermenting process and produces process.
1) fermenting process adds the water mill slurry with Fresh Folium Camelliae sinensis after kneading, the material-water ratio example is 1: 1-20, pack into then in the fermentor tank, in temperature is 30-40 ℃, pH value is bubbling air or oxygen under the condition of 5-6, stir, spontaneous fermentation 20-60 minute, obtain theoflavin solution, in order to improve the utilization ratio of Fresh Folium Camelliae sinensis, the aqueous extract or the tea-polyphenol that can add stem tea again carry out mixed fermentation, follow theoflavin solution by centrifugal slagging-off, tea grounds obtains fertilizer through the microbial fermentation drying, and centrifugate is carried out ultrafiltration through ceramic membrane and obtained filtrate; The membrane pore size of described ceramic membrane is 0.1-1 μ m.
2) producing process adopts solvent extration or membrane separation process to handle above-mentioned gained filtrate to obtain theoflavin.Described solvent extration is that filtrate is used organic solvent extraction, wherein solvent phase distills and reclaims organic solvent with 2-5% citric acid solution wash-out caffeine, and the theoflavin concentrated solution that obtains changes molten with pure water again, the spray-dried theoflavin that obtains, said organic solvent is an ethyl acetate.Described membrane separation process is that filtrate is separated with nanofiltration membrane, the fenestra of nanofiltration membrane be molecular weight between 200-10000 dalton, hold back the concentrated solution that obtains and handle with extraction process or resin adsorption method; Described extraction process is with the concentrated solution organic solvent extraction, and wherein solvent phase distills the theoflavin concentrated solution that obtains and changes molten with pure water again with 2-5% citric acid solution wash-out caffeine, and spray-dried or lyophilize obtains theoflavin; Described resin adsorption method is that concentrated solution is diluted to solid-to-liquid ratio is 1: the 10-20 upper prop, with macroporous adsorbent resin theoflavin is adsorbed, then with ethanol or propyl alcohol or the mixture wash-out of the two, Fractional Collections, distillating recovering solvent recycles, and theoflavin concentrated solution spraying drying or lyophilize are obtained theoflavin.Described organic solvent is an ethyl acetate.
Claims (6)
1, a kind of method for extracting theaflavin, it is characterized in that: Fresh Folium Camelliae sinensis is added the water mill slurry after kneading, the material-water ratio example is 1: 1-20, in the fermentor tank of packing into then, in temperature is 30-40 ℃, pH value is bubbling air or oxygen under the condition of 5-6, stirs spontaneous fermentation 20-60 minute, obtain theoflavin solution, then with theoflavin solution by centrifugal slagging-off, tea grounds obtains fertilizer through the microbial fermentation drying, centrifugate is carried out ultrafiltration through ceramic membrane and is obtained filtrate; Adopt solvent extration to handle gained filtrate, be about to filtrate and use organic solvent extraction, wherein solvent phase distills and reclaims organic solvent with 2-5% citric acid solution wash-out caffeine, the theoflavin concentrated solution that obtains changes molten with pure water again, the spray-dried theoflavin that obtains.
2, method for extracting theaflavin according to claim 1 is characterized in that: the aqueous extract or the tea-polyphenol that add stem tea during the fermentation carry out mixed fermentation.
3, method for extracting theaflavin according to claim 1 is characterized in that: the membrane pore size of described ceramic membrane is 0.1-1 μ m.
4, method for extracting theaflavin according to claim 1 is characterized in that: said organic solvent is an ethyl acetate.
5, method for extracting theaflavin according to claim 1, it is characterized in that: centrifugate is carried out the filtrate that ultrafiltration obtains through ceramic membrane and can be adopted membrane separation process to handle, being about to filtrate separates with nanofiltration membrane, the fenestra of nanofiltration membrane is that molecular weight is between 200-10000 dalton, holding back the concentrated solution that obtains handles with extraction process, be about to the concentrated solution organic solvent extraction, wherein solvent phase is with 2-5% citric acid solution wash-out caffeine, the theoflavin concentrated solution that distillation obtains changes molten with pure water again, and spray-dried or lyophilize obtains theoflavin.
6, method for extracting theaflavin according to claim 5, it is characterized in that: concentrated solution can adopt resin adsorption method to handle, being about to concentrated solution, to be diluted to solid-to-liquid ratio be 1: the 10-20 upper prop, with macroporous adsorbent resin theoflavin is adsorbed, then with ethanol or propyl alcohol or the mixture wash-out of the two, Fractional Collections, distillating recovering solvent recycles, and theoflavin concentrated solution spraying drying or lyophilize are obtained theoflavin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510075693 CN1289492C (en) | 2005-06-15 | 2005-06-15 | Method for extracting theaflavin |
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| CN 200510075693 CN1289492C (en) | 2005-06-15 | 2005-06-15 | Method for extracting theaflavin |
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| CN1699358A true CN1699358A (en) | 2005-11-23 |
| CN1289492C CN1289492C (en) | 2006-12-13 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096693B (en) * | 2007-07-26 | 2010-04-21 | 桂林莱茵生物科技股份有限公司 | Method for preparing theaflavin and thearubigin from fresh green tea |
| CN101974248A (en) * | 2010-09-10 | 2011-02-16 | 云南农业大学 | Method for separating tea pigment components |
| CN101081844B (en) * | 2006-06-01 | 2011-08-17 | 北京工商大学 | Method for large-batch separating preparation of high-purity theaflavine monomer |
| CN101691591B (en) * | 2009-09-30 | 2011-11-23 | 浙江派诺生物技术有限公司 | Industrial preparation method of theaflavin |
| CN102827896A (en) * | 2012-09-14 | 2012-12-19 | 向华 | Method for extracting theaflavin and theanine from tea leaves |
| CN104672195A (en) * | 2015-02-28 | 2015-06-03 | 中国农业科学院茶叶研究所 | Method of preparing theaflavins by virtue of centrifugal extraction and separation |
| CN109852646A (en) * | 2018-12-14 | 2019-06-07 | 成都华高生物制品有限公司 | A method of theaflavin is prepared using fresh tea leaf |
| CN111732566A (en) * | 2020-08-04 | 2020-10-02 | 大湾汉唯(广州)医药科技集团有限公司 | Method for extracting theaflavin |
| CN116076599A (en) * | 2023-03-13 | 2023-05-09 | 北京协同创新食品科技有限公司 | Tea extraction method and system |
-
2005
- 2005-06-15 CN CN 200510075693 patent/CN1289492C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101081844B (en) * | 2006-06-01 | 2011-08-17 | 北京工商大学 | Method for large-batch separating preparation of high-purity theaflavine monomer |
| CN101096693B (en) * | 2007-07-26 | 2010-04-21 | 桂林莱茵生物科技股份有限公司 | Method for preparing theaflavin and thearubigin from fresh green tea |
| CN101691591B (en) * | 2009-09-30 | 2011-11-23 | 浙江派诺生物技术有限公司 | Industrial preparation method of theaflavin |
| CN101974248A (en) * | 2010-09-10 | 2011-02-16 | 云南农业大学 | Method for separating tea pigment components |
| CN102827896A (en) * | 2012-09-14 | 2012-12-19 | 向华 | Method for extracting theaflavin and theanine from tea leaves |
| CN102827896B (en) * | 2012-09-14 | 2014-03-26 | 广州市一杰医药科技有限公司 | Method for extracting theaflavin and theanine from tea leaves |
| CN104672195A (en) * | 2015-02-28 | 2015-06-03 | 中国农业科学院茶叶研究所 | Method of preparing theaflavins by virtue of centrifugal extraction and separation |
| CN109852646A (en) * | 2018-12-14 | 2019-06-07 | 成都华高生物制品有限公司 | A method of theaflavin is prepared using fresh tea leaf |
| CN111732566A (en) * | 2020-08-04 | 2020-10-02 | 大湾汉唯(广州)医药科技集团有限公司 | Method for extracting theaflavin |
| CN111732566B (en) * | 2020-08-04 | 2020-11-27 | 大湾汉唯(广州)医药科技集团有限公司 | Method for extracting theaflavin |
| CN116076599A (en) * | 2023-03-13 | 2023-05-09 | 北京协同创新食品科技有限公司 | Tea extraction method and system |
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| Publication number | Publication date |
|---|---|
| CN1289492C (en) | 2006-12-13 |
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