CN1686469A - Medicament for treating coronary heart disease and its preparation method - Google Patents
Medicament for treating coronary heart disease and its preparation method Download PDFInfo
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Abstract
A Chinese medicine for treating coronary heart disease is prepared from ginseng, epimedium and one or more of safflower, Chuan-xiong rhizome, notoginseng, pueraria root, red sage root, gingko leaf, etc. Its preparing process is also disclosed.
Description
Technical field:
Field of medicaments of the present invention particularly relates to a kind of Chinese medicine preparation for the treatment of coronary heart disease and preparation method thereof, and said preparation is made up of Chinese medicine Radix Ginseng, Herba Epimedii and Chinese medicine with blood circulation promoting and blood stasis dispelling function, forms through extracting processing and preparing.
Background technology:
Cardiovascular disease is human disease and main causes of death as coronary heart disease, myocardial infarction, accounts for 1/3rd of general mortality rate, has surpassed cancer at its mortality rate of China.And along with China along with the raising of living standards of the people and the aging of population structure, the patient colony of cardiovascular and cerebrovascular disease is huge day by day.At present, the Chinese medicine and western medicine of treatment coronary heart disease is a lot, but how many Western medicine has some side effect.
Medicine of the present invention is applicable to the heart, spleen, insufficiency of kidney-YANG, blood stasis numbness resistance heart arteries and veins and the coronary heart disease that causes.Kidney is hidden fire from the gater of life, and basic for the whole body yang-energy, the yang-energy of other internal organs then can not be inspired, be rouse oneself to kidney yang deficiency, so hypoactivity of spleen-YANG, being difficult of pectoral qi given birth to, and deficiency of heart-YANG is agitated unable and hematogenous blockage.So sick this kidney yang deficiency can not rouse oneself yang-energy, the deficiency of vital energy can not agitate blood capable, should be based on the QI invigorating tonifying YANG, and being aided with the blood circulation promoting and blood stasis dispelling Tongxinluo is Therapeutic Method.Therefore, be monarch drug with Herba Epimedii, with kidney invigorating and YANG supporting, Radix Ginseng is a ministerial drug, QI of five ZANG-organs such as big reinforcing the heart, spleen, kidney, but supplementing QI to revive YANG like this, assistant is with having the Chinese medicine of blood circulation promoting and blood stasis dispelling function with the disperse blood stasis and dredge collateral pain relieving again.
The retrieval clinical report, Chinese traditional treatment coronary heart disease is the treatment rule with the blood circulation promoting and blood stasis dispelling, use treatment by Chinese herbs and obtained good effect, but emphasize blood circulation promoting and blood stasis dispelling for a long time simply, then can injure the human righteousness, the clinical curative effect instability that occurs, it is lower electrocardiogram improvement rate to occur, takes for a long time and causes the phenomenons such as breathing hard, recur the frequency height of having palpitation.Medicine of the present invention is in the QI invigorating tonifying YANG, on the basis of reinforcing body resistance, adopts the Therapeutic Method of blood circulation promoting and blood stasis dispelling, has avoided the appearance of above-mentioned phenomenon, stable curative effect.
Modern pharmacological research, the Radix Ginseng in the medicine of the present invention, Herba Epimedii all have the increase coronary flow, the effect that resists myocardial ischemia, the Chinese medicine with blood circulation promoting and blood stasis dispelling function also more has above effect, and this function for medicine of the present invention provides scientific basis.
Existing medicine belongs to a bit cures the symptoms, not the disease, and some uses expensive composition, and some is cut and interrupt using owing to uncertain therapeutic efficacy in application process.In view of this, the inventor develop a kind of taking convenience, effect steadily, determined curative effect, dosage form is stable, quality controllable and the treatment coronary heart disease pure Chinese medicinal preparation that has no side effect, to satisfy the demand of extensive patients.
The indication of medicine of the present invention is an angina pectoris, and the traditional Chinese medical science belongs to " cardiopalmus ", " thoracic obstruction " or " angina pectoris " etc., dialectical genus deficient and weak YANG QI, heart arteries and veins stasis of blood resistance person, disease is seen: sensation of oppression over the chest with shortness of breath, chest pain radiating to the back very then, cardiopalmus, aversion to cold and cold limbs, the soreness of waist, weak, pale complexion, pale or dark purple tongue, deep-thready pulse or faint pulse being about to disappear.
The bright medicine of we is not suitable for coronary heart disease, " cardiopalmus ", " thoracic obstruction " that Chinese medical discrimination belongs to deficiency of heart-yin.
Summary of the invention:
The invention provides a kind of Chinese medicine preparation for the treatment of coronary heart disease, said preparation is characterised in that with the Herba Epimedii to be monarch drug, and Radix Ginseng is a ministerial drug, and assistant forms through extracting processing and preparing with the Chinese medicine with blood circulation promoting and blood stasis dispelling function.The Chinese medicine that wherein has a blood blood stasis dispelling function can be selected from one or more of Flos Carthami, Rhizoma Chuanxiong, Radix Notoginseng, Radix Puerariae, Herba Erigerontis, Radix Salviae Miltiorrhizae, Folium Ginkgo.Said preparation is made by following Chinese medicinal raw materials:
Chinese medicine preparation of the present invention, in per 1000 dosage units, the weight portion of used raw material of Chinese medicine is as follows: 4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs and be selected from the Chinese medicine with blood circulation promoting and blood stasis dispelling function of following weight portion: 2000～8000 parts on Flos Carthami, 2000～8000 parts of Rhizoma Chuanxiongs, 2000～8000 parts of Radix Notoginseng, 2000～8000 parts of Radix Puerariaes, 2000～8000 parts of Herba Erigerontiss, 2000～8000 parts of Radix Salviae Miltiorrhizaes, 2000～8000 parts of Folium Ginkgos.
Preferred Chinese medicine preparation of the present invention, in per 1000 dosage units, the weight portion of used raw material of Chinese medicine is as follows: 6000～12000 parts of Herba Epimedii, 4000～8000 parts of Radix Ginsengs and be selected from the Chinese medicine with blood circulation promoting and blood stasis dispelling function of following weight portion: 3000～6000 parts on Flos Carthami, 3000～6000 parts of Rhizoma Chuanxiongs, 3000～6000 parts of Radix Notoginseng, 3000～6000 parts of Radix Puerariaes, 3000～6000 parts of Herba Erigerontiss, 3000～6000 parts of Radix Salviae Miltiorrhizaes, 3000～6000 parts of Folium Ginkgos.
Particularly preferred Chinese medicine preparation of the present invention, in per 1000 dosage units, the weight portion of used raw material of Chinese medicine is as follows: 8000 parts of Herba Epimedii, 5000 parts of Radix Ginsengs and be selected from the Chinese medicine with blood circulation promoting and blood stasis dispelling function of following weight portion: 4000 parts on Flos Carthami, 4000 parts of Rhizoma Chuanxiongs, 4000 parts of Radix Notoginseng, 4000 parts of Radix Puerariaes, 4000 parts of Herba Erigerontiss, 4000 parts of Radix Salviae Miltiorrhizaes, 4000 parts of Folium Ginkgos.
Chinese medicine preparation prescription 1 proportioning of the present invention (weight portion) is in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts on Flos Carthami;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts on Flos Carthami;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts on Flos Carthami.
Chinese medicine preparation prescription 2 proportionings of the present invention (weight portion) are in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts of Rhizoma Chuanxiongs;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts of Rhizoma Chuanxiongs;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts of Rhizoma Chuanxiongs.
Chinese medicine preparation prescription 3 proportionings of the present invention (weight portion) are in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts of Radix Notoginseng;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts of Radix Notoginseng;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts of Radix Notoginseng.
Chinese medicine preparation prescription 4 proportionings of the present invention (weight portion) are in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts of Radix Puerariaes;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts of Radix Puerariaes;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts of Radix Puerariaes.
Chinese medicine preparation prescription 5 proportionings of the present invention (weight portion) are in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts of Herba Erigerontiss;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts of Herba Erigerontiss;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts of Herba Erigerontiss.
Chinese medicine preparation prescription 6 proportionings of the present invention (weight portion) are in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts of Radix Salviae Miltiorrhizaes;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts of Radix Salviae Miltiorrhizaes;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts of Radix Salviae Miltiorrhizaes.
Chinese medicine preparation prescription 7 proportionings of the present invention (weight portion) are in crude drug:
4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs, 2000～8000 parts of Folium Ginkgos;
Preferably Herba Epimedii is 6000～12000 parts, 4000～8000 parts of Radix Ginsengs, 3000～6000 parts of Folium Ginkgos;
More preferably Herba Epimedii is 8000 parts, 5000 parts of Radix Ginsengs, 4000 parts of Folium Ginkgos.
In more than forming, the weight of medicine is calculated with crude drug, and per 1 part can be 1 gram, also can be kilogram or ton, if be unit with gram, this prescription composition can be made into 1000 doses of pharmaceutical preparatioies.Described 1000 doses of fingers, the final drug preparation of making, as make 1000 of capsule preparations, 1000 in tablet, granule 1000g, oral liquid 1000ml etc., also can make big packing as granule, as 100～500 bags, specifically can be 100 bags, 125 bags, 200 bags, 250 bags, 500 bags etc., every bag can be used as taking dose 1 time.
More than form, can be made into the preparation of 50～1000 taking doses, as capsule, make 1000, each taking dose can be 1～8, can take altogether 125～1000 times.As granule, make 125 bags, take 1～2 bag at every turn, can take altogether 62.5～125 times.
More than form to be by weight as proportioning, when producing, can increase or reduce according to corresponding proportion, as large-scale production can be unit with the kilogram, or be unit with the ton, small-scale production can be unit with the milligram also, weight can increase or reduce, but the constant rate of the raw medicinal herbs weight proportion between each composition.
The ratio of above weight proportion obtains through science screening, for especial patient, and as serious symptom or light disease, fat or modest patient, the proportioning of the amount of can corresponding adjustment forming increases or reduces being no more than 100%, and drug effect is constant.
Chinese medicine preparation of the present invention is to process through extraction or other modes by the raw material of Chinese medicine that above-mentioned prescription is formed, and makes pharmaceutically active substance, subsequently, with this material is raw material, adds the medicine acceptable carrier when needing, and makes according to the routine techniques of galenic pharmacy.Described active substance can obtain by extracting raw material of Chinese medicine respectively, also can obtain by the co-extracted raw material of Chinese medicine, also can obtain by other modes, as: by pulverize, squeeze, calcine, grind, sieve, percolation, extraction, water are carried, alcohol extraction, ester are carried, methods such as ketone is carried, chromatography obtain, these active substances can be the material of extractum form, can be that dry extract also can be a fluid extract, make different concentration according to the different needs decision of preparation.
Pharmaceutically active substance in the pharmaceutical preparation of the present invention, its shared percentage by weight in preparation can be 0.1～99.9%, all the other are the medicine acceptable carrier.Pharmaceutical preparation of the present invention exists with unit dosage form, and described unit dosage form is meant the unit of preparation, as every of tablet, capsular every capsules, every of injection etc., in the unit dose, the amount that contains active substance is 5～800mg, preferably 20～500mg.
Pharmaceutical preparation of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, capsule, oral liquid, syrup, granule, pill, powder, unguentum, sublimed preparation, injection, suppository, cream, spray, drop pill, patch, slow releasing preparation, controlled release preparation.
Pharmaceutical preparation of the present invention, the preparation of its oral administration can contain excipient commonly used, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet in case of necessity.
The filler that is suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant, for example magnesium stearate.The acceptable wetting agent of appropriate drug comprises sodium lauryl sulphate.
Can fill by mixing, the method that tabletting etc. are commonly used prepares solid oral composition.Mix repeatedly active substance is distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid for example can be aqueous or oily suspensions, solution, Emulsion, syrup or elixir, perhaps can be a kind of available water before use or other suitable composite dry products of carrier.This liquid preparation can contain conventional additive, such as suspending agent, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat, emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if desired, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this chemical compound can be suspended or dissolving.The preparation of solution is normally by being dissolved in active substance in a kind of carrier filter-sterilized before it is packed into a kind of suitable bottle or ampoule, sealing then.For example a kind of local anesthetic of adjuvant, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after the bottle of packing into that this compositions is freezing, and under vacuum, water is removed.
Pharmaceutical preparation of the present invention, when being prepared into medicament, optionally add suitable medicine acceptable carrier, described medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, the EDTA disodium, EDTA calcium sodium, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, the phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Pharmaceutical preparation of the present invention, active component can extract a few flavor medicines respectively, and extraction also can mix.When extracting respectively, method is as follows:
The preparation method of Radix Ginseng active component: take by weighing the ginseng crude drug, add 65～75% ethanol extractions twice, extracting solution has reclaimed ethanol, leaves standstill centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse ethanol is resolved, and desorbed solution concentrates after drying, and dried cream adds 90～95% ethanol extraction, and the extracting solution concentrate drying gets the Radix Ginseng active component;
The preparation method of Herba Epimedii active component: take by weighing epimedium herb, add 65～75% ethanol extractions twice, extracting solution has reclaimed ethanol, adds to leave standstill centrifugally after 2～3 times of water gagings stir, and centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 60～70% ethanol, and desorbed solution concentrates after drying, and dried cream adds 90～95% ethanol extraction, and the extracting solution concentrate drying gets the Herba Epimedii active component;
The preparation method of Flos Carthami active component: take by weighing Flos Carthami, add water temperature and soak secondary, warm macerating liquid is centrifugal, and centrifugal liquid is crossed non-polar macroporous resin, carries out eluting, and eluent is crossed detached dowel, gets the Flos Carthami active component;
The preparation method of Rhizoma Chuanxiong active component: take by weighing the Rhizoma Chuanxiong medical material, the AB-8 macroporous resin adsorption is crossed in the centrifugal while hot back of extracting in water twice, extracting solution, washes with water to remove assortedly, resolves desorbed solution concentrating under reduced pressure, dry Rhizoma Chuanxiong active component with 50～80% ethanol;
The preparation method of pseudo-ginseng activity composition: get Radix Notoginseng and add 65～75% ethanol extractions, decompression recycling ethanol, D is crossed in centrifugal back
101Macroporous resin column absorption washes with water and removes assortedly, resolves with 50～80% ethanol, and the desorbed solution concentrating under reduced pressure must the pseudo-ginseng activity composition after eluent concentrates;
The preparation method of Radix Puerariae active component: get Radix Puerariae and add 65～75% ethanol extractions twice, decompression recycling ethanol adds 2～5 times of water gagings and is heated to 45～65 ℃ of fully dissolvings to doing, filtered while hot, and 45～55 ℃ of conditions of filtrate were descended D
101Macroporous resin column absorption washes with water and removes assortedly, and reuse 60～70% ethanol are resolved, and desorbed solution is crossed polyamide column after having reclaimed ethanol, uses the Different concentrations of alcohol gradient elution, and recrystallization got the Radix Puerariae active component after eluent was concentrated;
The preparation method of Herba Erigerontis active component: it is an amount of to get Herba Erigerontis, with 75% ethanol merceration of 3～4 times of amounts three times, the cooling bath concentrating under reduced pressure becomes extractum, and heating aqueous alkali adjust pH is to about 6.5, filter while hot, filtrate adds sulphuric acid adjust pH to 2.0～2.5, leaves standstill 48 hours, separates out precipitate, the precipitate washing with alcohol, use recrystallizing methanol, the crystal vacuum drying is volatilized methanol, promptly get the Herba Erigerontis active component;
The preparation of Folium Ginkgo active component: take by weighing Folium Ginkgo, add 65～75% ethanol extraction secondary, extracting solution has reclaimed ethanol, adds to leave standstill centrifugally after water stirs, and centrifugal liquid is crossed DM
130Macroporous resin, the water eluting, reuse ethanol is resolved, and desorbed solution concentrates the back spray drying, and dried cream adds the height ethanol extraction, and the extracting solution concentrate drying gets dry extract; And adopt extraction to remove the harmful substance ginkgoic acid to get the Folium Ginkgo active component;
The preparation of Radix Salviae Miltiorrhizae active component: take by weighing Radix Salviae Miltiorrhizae, adding water temperature soaks, warm macerating liquid is centrifugal, centrifugal liquid is evaporated to 1/5 of original volume, adds acid and transfers PH to 2～3, the ethyl acetate extraction of usefulness equivalent 2 times, acetic acid ethyl fluid is evaporated on a small quantity, leave standstill and take off a layer slag, lower floor's slag recrystallization gets the salvianolic acid constituents.
The beneficial effect of following experimentation explanation medicine of the present invention:
Experimentation one, Herba Epimedii, Radix Ginseng, safflower capsules (hereinafter to be referred as celestial being ginseng Air Bladder pseudosciaenae seu Acipenser capsule) are to the protective effect of myocardial ischemia
Get the Wistar rat, be divided into 3 groups at random, be i.e. model control group, celestial ginseng flower Capsules group, XINNAOSHUTONG JIAONANG matched group.Model control group is irritated stomach and is given distilled water (4ml/kg), and all the other each groups are irritated the corresponding medicinal liquid of stomach respectively by table 1 dosage.Be administered once every day, continuous 6 days.Behind the last administration 1h, urethane (5%, face upward the position and fix by 20ml/kg) anesthetized rat, cut off animal skin along midsternal line, at left border of sternum 6～7 intercostal openings, extrude heart rapidly after, ligation at once left side heart coronary artery anterior descending branch root, heart is put back to the thoracic cavity, close thoracic cavity and stitching, take out heart behind the 6h ,-20 ℃ of preservations are spent the night.In the experimentation, respectively at (normally), ligation before the operation at once, 2h, 6h recording ecg after the ligation, observe that the ST section changes and myocardial ischemia improvement situation.The results are shown in Table 1.
The celestial ginseng of table 1 Air Bladder pseudosciaenae seu Acipenser capsule is to the protective effect of myocardial ischemia
| Group | ????N | Dosage mg/kg | ST section change absolute value (x ± s) | Myocardial ischemia scope ratio | ||
| Ligation at once | ????2h | ???6h | ||||
| The celestial ginseng flower of model group Capsules group XINNAOSHUTONG JIAONANG group | ????14 ????18 ????17 | ????- ????150 ????100 | 2.43±1.03 2.45±1.25 2.47±1.34 | 2.24±1.14 1.25±1.27 *#△1.76±1.24 | 2.54±1.06 1.13±1.18 **#△1.62±1.28 * | 0.35±0.23 0.14±0.06 **#△0.19±0.07 ** |
Annotate: compare with model group,
*P＜0.05,
*P＜0.01.
Compare with the XINNAOSHUTONG JIAONANG group,
#P＜0.05.
As shown in Table 1, celestial ginseng Air Bladder pseudosciaenae seu Acipenser capsule is significantly improved to the ECG ST section ischemic change behind the ligation 2h, celestial ginseng Air Bladder pseudosciaenae seu Acipenser capsule, XINNAOSHUTONG JIAONANG group are also significantly improved its variation during 6h, and celestial ginseng flower Capsules group is better than XINNAOSHUTONG JIAONANG matched group (P＜0.05, P＜0.05) in the improvement situation of each time point.Celestial ginseng flower Capsules group also is better than the XINNAOSHUTONG JIAONANG group aspect the myocardial ischemia scope alleviating.
Experimentation two, Herba Epimedii, Radix Ginseng, Rhizoma Chuanxiong injection (hereinafter to be referred as celestial being ginseng Rhizoma Chuanxiong injection) are to the protective effect of myocardial ischemia
Get the Wistar rat, be divided into 3 groups at random, be i.e. model control group, celestial ginseng Rhizoma Chuanxiong injection group, XUESHUANTONG ZHUSHEYE matched group.Model control group intraperitoneal injection of saline (4ml/kg), all the other each groups are pressed the table 2 dosage corresponding medicinal liquid of lumbar injection respectively, and be administered once every day, continuous 6 days.All the other experimental techniques are described with " experimentation one ".The results are shown in Table 2.
The celestial ginseng of table 2 Rhizoma Chuanxiong injection is to the protective effect of myocardial ischemia
| Group | ??N | Dosage mg/kg | ST section change absolute value (x ± s) | Myocardial ischemia scope ratio | ||
| Ligation at once | ??2h | ????6h | ||||
| The celestial ginseng of model group Rhizoma Chuanxiong injection group XUESHUANTONG ZHUSHEYE group | ??15 ??19 ??17 | ????- ????100 ????80 | ??2.45±1.07 ??2.43±1.15 ??2.45±1.12 | 2.28±1.16 1.27±1.14 *#△1.73±1.22 | 2.62±1.26 1.08±1.20 **#△1.66±1.18 * | 0.36±0.34 0.13±0.07 **#△0.20±0.03 ** |
Annotate: compare with model group,
*P＜0.05,
*P＜0.01.
Compare with the XUESHUANTONG ZHUSHEYE group,
#P＜0.05.
As seen from the above table, celestial ginseng Rhizoma Chuanxiong injection is significantly improved to the ECG ST section ischemic change behind the ligation 2h, celestial ginseng Rhizoma Chuanxiong injection, XUESHUANTONG ZHUSHEYE group are also significantly improved its variation during 6h, and celestial ginseng Rhizoma Chuanxiong injection group is better than XUESHUANTONG ZHUSHEYE matched group (P＜0.05, P＜0.05) in the improvement situation of each time point.Celestial ginseng Rhizoma Chuanxiong injection group also is better than the XUESHUANTONG ZHUSHEYE group aspect the myocardial ischemia scope alleviating.
Experimentation three, Herba Epimedii, Radix Ginseng, Radix Notoginseng Tabellae (hereinafter to be referred as celestial Radix Notoginseng sheet) are to the protective effect of myocardial ischemia
Get the Wistar rat, be divided into 3 groups at random, be i.e. model control group, celestial Radix Notoginseng sheet, XINNAOSHUTONG JIAONANG matched group.Model control group is irritated stomach distilled water (4ml/kg), and all the other each groups are irritated the corresponding medicinal liquid of stomach respectively by table 3 dosage, and be administered once every day, continuous 6 days.All the other experimental techniques are described with " experimentation one ".The results are shown in Table 3.
The celestial Radix Notoginseng sheet of table 3 is to the protective effect of myocardial ischemia
| Group | ??N | Dosage mg/kg | ST section change absolute value (x ± s) | Myocardial ischemia scope ratio | ||
| Ligation at once | ??2h | ????6h | ||||
| The celestial Radix Notoginseng sheet of model group XINNAOSHUTONG JIAONANG group | ?13 ?18 ?16 | ????- ????150 ????100 | ????2.48±1.13 ????2.46±1.18 ????2.42±1.12 | 2.32±1.21 1.29±1.07 *#△1.69±1.15 | 2.67±1.24 1.04±1.03 **#△1.61±1.14 * | 0.35±0.68 0.12±0.07 **#△0.18±0.23 ** |
Annotate: compare with model group,
*P＜0.05,
*P＜0.01.
Compare with the XINNAOSHUTONG JIAONANG group,
#P＜0.05.
As shown in Table 3, celestial Radix Notoginseng sheet is significantly improved to the ECG ST section ischemic change behind the ligation 2h, celestial Radix Notoginseng sheet, XINNAOSHUTONG JIAONANG group are also significantly improved its variation during 6h, and celestial Radix Notoginseng sheet is better than XINNAOSHUTONG JIAONANG matched group (P＜0.05, P＜0.05) in the improvement situation of each time point.Celestial Radix Notoginseng sheet also is better than the XINNAOSHUTONG JIAONANG group aspect the myocardial ischemia scope alleviating.
The specific embodiment
Further specify the present invention by the following examples, but not as limitation of the present invention.
Embodiment 1
It is characterized in that containing three kinds of Chinese medicines, Herba Epimedii, Radix Ginseng and Flos Carthami.
The preparation method of injection is as follows:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, must the Herba Epimedii active component behind the intact ethanol of extracting solution reclaim under reduced pressure;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, water eluting, reuse 70% ethanol are resolved, desorbed solution concentrates the back spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, extracting solution be concentrated into no alcohol distinguish the flavor of the Radix Ginseng active component;
The preparation method of Flos Carthami active component: take by weighing Flos Carthami 4000g, add 12 times of amounts, 10 times of water gagings respectively in 80 ℃ of warm macerating secondaries, warm macerating liquid is centrifugal by tubular type, and centrifugal liquid is crossed non-polar macroporous resin, carries out eluting, and eluent is crossed detached dowel, gets the Flos Carthami active component;
Get above-mentioned three kinds of active component and add water for injection, heated and stirred makes dissolving, and adjust pH adds active carbon heating 15 minutes, cooling filters, and adds the volume that the injection water is settled to regulation, with the 0.2 μ m microporous filter membrane fine straining of sterilizing, degerming, bottle 1000 (600mg/ props up).
The preparation method of capsule preparations:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, and the extracting solution reclaim under reduced pressure is finished the ethanol after drying and is got Herba Epimedii active component 228g;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse 70% ethanol is resolved, the concentrated back of desorbed solution spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, and extracting solution is concentrated into does not have the dry Radix Ginseng active component 167g of getting of alcohol flavor;
The preparation method of Flos Carthami active component: take by weighing Flos Carthami 4000g, add 12 times of amounts, 10 times of water gagings respectively in 80 ℃ of warm macerating secondaries, warm macerating liquid is centrifugal by tubular type, centrifugal liquid is crossed non-polar macroporous resin, carry out eluting, eluent is crossed detached dowel, gets Flos Carthami active component 42.5g;
Get that above three kinds of active component are pulverized, behind the mix homogeneously, add a certain amount of adjuvant mix homogeneously and incapsulate, make 1000 capsules (0.5g/ grain), reinstall in the bottle after aluminum-plastic packaged and seal.
The preparation method of granular preparation is as follows:
The preparation method of tablet is as follows:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, and the extracting solution reclaim under reduced pressure is finished the ethanol after drying and is got Herba Epimedii active component 219g;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse 70% ethanol is resolved, the concentrated back of desorbed solution spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, and extracting solution is concentrated into does not have the dry Radix Ginseng active component 171g of getting of alcohol flavor;
The preparation method of Flos Carthami active component: take by weighing Flos Carthami 4000g, add 12 times of amounts, 10 times of water gagings respectively in 80 ℃ of warm macerating secondaries, warm macerating liquid is centrifugal by tubular type, centrifugal liquid is crossed non-polar macroporous resin, carry out eluting, eluent is crossed detached dowel, gets Flos Carthami active component 41.7g;
After getting above three kinds of active component pulverizing, mix homogeneously, add a certain amount of adjuvant mix homogeneously, make soft material in right amount with ethanol, the system granule, cold drying, tabletting behind the granulate gets 1000 (0.5g/ sheet).
Embodiment 2
It is characterized in that containing three kinds of Chinese medicines, Herba Epimedii, Radix Ginseng and Rhizoma Chuanxiong.
The preparation method of injection is as follows:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, must the Herba Epimedii active component behind the intact ethanol of extracting solution reclaim under reduced pressure;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, water eluting, reuse 70% ethanol are resolved, desorbed solution concentrates the back spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, extracting solution be concentrated into no alcohol distinguish the flavor of the Radix Ginseng active component;
The preparation method of Rhizoma Chuanxiong active component: take by weighing Rhizoma Chuanxiong medical material 4000g, add 10 times of amounts, 8 times of water gagings extractions twice respectively, the AB-8 macroporous resin adsorption is crossed in the centrifugal while hot back of extracting solution, wash with water after the absorption remove assorted, resolve with 75% ethanol, the desorbed solution reclaim under reduced pressure is finished ethanol, gets the Rhizoma Chuanxiong active component;
Get above-mentioned three kinds of active component and add water for injection, heated and stirred makes dissolving, and adjust pH adds active carbon heating 15 minutes, cooling filters, and adds the volume that the injection water is settled to regulation, with the 0.2 μ m microporous filter membrane fine straining of sterilizing, degerming, bottle 1000 (600mg/ props up).
The preparation method of capsule preparations:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, and the extracting solution reclaim under reduced pressure is finished the ethanol after drying and is got Herba Epimedii active component 231g;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse 70% ethanol is resolved, the concentrated back of desorbed solution spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, and extracting solution is concentrated into does not have the dry Radix Ginseng active component 171g of getting of alcohol flavor;
The preparation method of Rhizoma Chuanxiong active component: take by weighing Rhizoma Chuanxiong medical material 4000g, add 10 times of amounts respectively, 8 times of water gagings extract twice, the AB-8 macroporous resin adsorption is crossed in the centrifugal while hot back of extracting solution, wash with water after the absorption remove assorted, resolve with 75% ethanol, the desorbed solution reclaim under reduced pressure is finished ethanol, gets Rhizoma Chuanxiong active component 51.7g;
Get that above three kinds of active component are pulverized, behind the mix homogeneously, add a certain amount of adjuvant mix homogeneously and incapsulate, make 1000 capsules (0.5g/ grain), reinstall in the bottle after aluminum-plastic packaged and seal.
The preparation method of granular preparation is as follows:
The preparation method of tablet is as follows:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, and the extracting solution reclaim under reduced pressure is finished the ethanol after drying and is got Herba Epimedii active component 229g;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse 70% ethanol is resolved, the concentrated back of desorbed solution spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, and extracting solution is concentrated into does not have the dry Radix Ginseng active component 176g of getting of alcohol flavor;
The preparation method of Rhizoma Chuanxiong active component: take by weighing Rhizoma Chuanxiong medical material 4000g, add 10 times of amounts respectively, 8 times of water gagings extract twice, the AB-8 macroporous resin adsorption is crossed in the centrifugal while hot back of extracting solution, wash with water after the absorption remove assorted, resolve with 75% ethanol, the desorbed solution reclaim under reduced pressure is finished ethanol, gets Rhizoma Chuanxiong active component 49.8g; After getting above three kinds of active component pulverizing, mix homogeneously, add a certain amount of adjuvant mix homogeneously, make soft material in right amount with ethanol, the system granule, cold drying, tabletting behind the granulate gets 1000 (0.5g/ sheet).
Embodiment 3
It is characterized in that containing three kinds of Chinese medicines, Herba Epimedii, Radix Ginseng and Radix Notoginseng.
The preparation method of injection is as follows:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, must the Herba Epimedii active component behind the intact ethanol of extracting solution reclaim under reduced pressure;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, water eluting, reuse 70% ethanol are resolved, desorbed solution concentrates the back spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, extracting solution be concentrated into no alcohol distinguish the flavor of the Radix Ginseng active component;
The preparation method of pseudo-ginseng activity composition: get Radix Notoginseng and add 65～75% ethanol extractions, decompression recycling ethanol, D is crossed in centrifugal back
101Macroporous resin column absorption washes with water and removes assortedly, resolves with 50～80% ethanol, and the desorbed solution concentrating under reduced pressure must the pseudo-ginseng activity composition after eluent concentrates;
Get above-mentioned three kinds of active component and add water for injection, heated and stirred makes dissolving, and adjust pH adds active carbon heating 15 minutes, cooling filters, and adds the volume that the injection water is settled to regulation, with the 0.2 μ m microporous filter membrane fine straining of sterilizing, degerming, bottle 1000 (600mg/ props up).
The preparation method of capsule preparations:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, and the extracting solution reclaim under reduced pressure is finished the ethanol after drying and is got Herba Epimedii active component 240g;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse 70% ethanol is resolved, the concentrated back of desorbed solution spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, and extracting solution is concentrated into does not have the dry Radix Ginseng active component 168g of getting of alcohol flavor;
The preparation method of pseudo-ginseng activity composition: get Radix Notoginseng and add 65～75% ethanol extractions, decompression recycling ethanol, D is crossed in centrifugal back
101Macroporous resin column absorption washes with water and removes assortedly, resolves with 50～80% ethanol, and the desorbed solution concentrating under reduced pressure must pseudo-ginseng activity composition 81.2g after eluent concentrates;
Get that above three kinds of active component are pulverized, behind the mix homogeneously, add a certain amount of adjuvant mix homogeneously and incapsulate, make 1000 capsules (0.5g/ grain), reinstall in the bottle after aluminum-plastic packaged and seal.
The preparation method of granular preparation is as follows:
The preparation method of tablet is as follows:
The preparation method of Herba Epimedii active component: take by weighing epimedium herb 8000g, add 70% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, the extracting solution reclaim under reduced pressure is finished ethanol, add and leave standstill 4 hours after 2 times of water gagings stir, centrifugal by tubular type, centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 70% ethanol, spray drying behind the desorbed solution concentrating under reduced pressure, dried cream adds 90% ethanol extraction of 10 times of amounts, and the extracting solution reclaim under reduced pressure is finished the ethanol after drying and is got Herba Epimedii active component 229g;
The preparation method of Radix Ginseng active component: take by weighing ginseng crude drug 5000g, add 65% ethanol extraction twice, the ethanol consumption is respectively 7 times of amounts, 5 times of amounts, and the extracting solution reclaim under reduced pressure is finished ethanol, leaves standstill after 4 hours by tubular type centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse 70% ethanol is resolved, the concentrated back of desorbed solution spray drying, dried cream adds 90% ethanol extraction of 10 times of amounts, and extracting solution is concentrated into does not have the dry Radix Ginseng active component 176g of getting of alcohol flavor;
The preparation method of pseudo-ginseng activity composition: get Radix Notoginseng and add 65～75% ethanol extractions, decompression recycling ethanol, D is crossed in centrifugal back
101Macroporous resin column absorption washes with water and removes assortedly, resolves with 50～80% ethanol, and the desorbed solution concentrating under reduced pressure must pseudo-ginseng activity composition 89.8g after eluent concentrates;
After getting above three kinds of active component pulverizing, mix homogeneously, add a certain amount of adjuvant mix homogeneously, make soft material in right amount with ethanol, the system granule, cold drying, tabletting behind the granulate gets 1000 (0.5g/ sheet).
Embodiment 4
For the preparation that contains Chinese medicine Herba Epimedii, Radix Ginseng and Radix Puerariae, wherein the preparation method of Herba Epimedii and Radix Ginseng extract is identical with embodiment 1, and Radix Puerariae extract is prepared as follows:
Take by weighing Radix Puerariae 4000g, add 65～75% ethanol extractions twice, decompression recycling ethanol adds 2～5 times of water gagings and is heated to 45～65 ℃ of fully dissolvings to doing, filtered while hot, and 45～55 ℃ of conditions of filtrate were descended D
101Macroporous resin column absorption washes with water and removes assortedly, and reuse 60～70% ethanol are resolved, and desorbed solution is crossed polyamide column after having reclaimed ethanol, uses the Different concentrations of alcohol gradient elution, and recrystallization got the Radix Puerariae active component after eluent was concentrated;
Add 12 times of amounts, 10 times of water gagings respectively in 80 ℃ of warm macerating secondaries, warm macerating liquid is centrifugal by tubular type, and centrifugal liquid is crossed non-polar macroporous resin, carries out eluting, and eluent is crossed detached dowel, gets the Radix Puerariae active component.
The Herba Epimedii, Radix Ginseng and the Radix Puerariae active component that extract are mixed, be prepared into preparation according to the galenic pharmacy routine techniques.
Embodiment 5
For the preparation that contains Chinese medicine Herba Epimedii, Radix Ginseng and Herba Erigerontis, wherein the preparation method of Herba Epimedii and Radix Ginseng extract is identical with embodiment 1, and Herba Erigerontis extract is prepared as follows:
Take by weighing Herba Erigerontis 4000g, with 75% ethanol merceration of 3～4 times of amounts three times, the cooling bath concentrating under reduced pressure becomes extractum, and heating aqueous alkali adjust pH is to about 6.5, filter while hot, filtrate adds sulphuric acid adjust pH to 2.0～2.5, leaves standstill 48 hours, separates out precipitate, the precipitate washing with alcohol, use recrystallizing methanol, the crystal vacuum drying is volatilized methanol, promptly get the Herba Erigerontis active component;
The Herba Epimedii, Radix Ginseng and the Herba Erigerontis active component that extract are mixed, be prepared into preparation according to the galenic pharmacy routine techniques.
Embodiment 6
For the preparation that contains Chinese medicine Herba Epimedii, Radix Ginseng and Radix Salviae Miltiorrhizae, wherein the preparation method of Herba Epimedii and Radix Ginseng extract is identical with embodiment 1, and Radix Salviae Miltiorrhizae extract is prepared as follows:
Take by weighing Radix Salviae Miltiorrhizae 4000g, the preparation of Radix Salviae Miltiorrhizae active component: take by weighing Radix Salviae Miltiorrhizae, add water temperature and soak, warm macerating liquid is centrifugal, and centrifugal liquid is evaporated to 1/5 of original volume, add acid and transfer PH to 2～3, with the ethyl acetate extraction of equivalent 2 times, acetic acid ethyl fluid is evaporated on a small quantity, leaves standstill to take off a layer slag, lower floor's slag recrystallization gets the salvianolic acid constituents.
The Herba Epimedii, Radix Ginseng and the Radix Salviae Miltiorrhizae active component that extract are mixed, be prepared into preparation according to the galenic pharmacy routine techniques.
Embodiment 7
For the preparation that contains Chinese medicine Herba Epimedii, Radix Ginseng and Folium Ginkgo, wherein the preparation method of Herba Epimedii and Radix Ginseng extract is identical with embodiment 1, and Folium Ginkgo extract is prepared as follows:
Take by weighing Folium Ginkgo 4000g, add 65～75% ethanol extraction secondary, extracting solution has reclaimed ethanol, adds to leave standstill centrifugally after water stirs, and centrifugal liquid is crossed DM
130Macroporous resin, the water eluting, reuse ethanol is resolved, and desorbed solution concentrates the back spray drying, and dried cream adds the height ethanol extraction, and the extracting solution concentrate drying gets dry extract; And adopt extraction to remove the harmful substance ginkgoic acid to get ginkgetin and total lactone;
The Herba Epimedii, Radix Ginseng and the Folium Ginkgo active component that extract are mixed, be prepared into preparation according to the galenic pharmacy routine techniques.
Claims (10)
1. a Chinese medicine preparation for the treatment of coronary heart disease is characterized in that, said preparation is by Herba Epimedii, and Radix Ginseng and one or more Chinese medicines with blood circulation promoting and blood stasis dispelling function are made.
2. the Chinese medicine preparation of claim 1 is characterized in that, described Chinese medicine with blood circulation promoting and blood stasis dispelling function is selected from one or more of Flos Carthami, Rhizoma Chuanxiong, Radix Notoginseng, Radix Puerariae, Herba Erigerontis, Radix Salviae Miltiorrhizae, Folium Ginkgo.
3. the Chinese medicine preparation of claim 2, it is characterized in that, per 1000 dosage units are made by following Chinese medicinal raw materials: 4000～16000 parts of Herba Epimedii, 2500～10000 parts of Radix Ginsengs and be selected from the Chinese medicine with blood circulation promoting and blood stasis dispelling function of following weight portion: 2000～8000 parts on Flos Carthami, 2000～8000 parts of Rhizoma Chuanxiongs, 2000～8000 parts of Radix Notoginseng, 2000～8000 parts of Radix Puerariaes, 2000～8000 parts of Herba Erigerontiss, 2000～8000 parts of Radix Salviae Miltiorrhizaes, 2000～8000 parts of Folium Ginkgos.
4. the Chinese medicine preparation of claim 2, it is characterized in that per 1000 dosage units are made by following Chinese medicinal raw materials: 6000～12000 parts of Herba Epimedii, 4000～8000 parts of Radix Ginsengs, with the Chinese medicine that is selected from following weight portion with blood circulation promoting and blood stasis dispelling function: 3000～6000 parts on Flos Carthami, 3000～6000 parts of Rhizoma Chuanxiongs, 3000～6000 parts of Radix Notoginseng, 3000～6000 parts of Radix Puerariaes, 3000～6000 parts of Herba Erigerontiss, 3000～6000 parts of Radix Salviae Miltiorrhizaes, 3000～6000 parts of Folium Ginkgos.
5. the Chinese medicine preparation of claim 2, it is characterized in that per 1000 dosage units are made by following Chinese medicinal raw materials: 8000 parts of Herba Epimedii, 5000 parts of Radix Ginsengs, with the Chinese medicine that is selected from following weight portion with blood circulation promoting and blood stasis dispelling function: 4000 parts on Flos Carthami, 4000 parts of Rhizoma Chuanxiongs, 4000 parts of Radix Notoginseng, 4000 parts of Radix Puerariaes, 4000 parts of Herba Erigerontiss, 4000 parts of Radix Salviae Miltiorrhizaes, 4000 parts of Folium Ginkgos.
6. the Chinese medicine preparation of claim 5 is characterized in that per 1000 dosage units are made by following Chinese medicinal raw materials: 8000 parts of Herba Epimedii, 5000 parts of Radix Ginsengs, 4000 parts on Flos Carthami.
7. the Chinese medicine preparation of claim 5 is characterized in that per 1000 dosage units are made by following Chinese medicinal raw materials: 8000 parts of Herba Epimedii, 5000 parts of Radix Ginsengs, 4000 parts of Rhizoma Chuanxiongs.
8. the Chinese medicine preparation of claim 5 is characterized in that per 1000 dosage units are made by following Chinese medicinal raw materials: 8000 parts of Herba Epimedii, 5000 parts of Radix Ginsengs, 4000 parts of Radix Notoginseng.
9. the Chinese medicine preparation of claim 5 is characterized in that per 1000 dosage units are made by following Chinese medicinal raw materials: 8000 parts of Herba Epimedii, 5000 parts of Radix Ginsengs, 4000 parts of 4000 parts of 4000 parts of 4000 parts of Radix Puerariaes or Herba Erigerontiss or Radix Salviae Miltiorrhizaes or Folium Ginkgos.
10. the preparation method of the Chinese medicine preparation of claim 2 is characterized in that, may further comprise the steps:
The preparation method of Radix Ginseng active component: take by weighing the ginseng crude drug, add 65～75% ethanol extractions twice, extracting solution has reclaimed ethanol, leaves standstill centrifugally, and centrifugal liquid is crossed D
101Macroporous resin, the water eluting, reuse ethanol is resolved, and desorbed solution concentrates after drying, and dried cream adds 90～95% ethanol extraction, and the extracting solution concentrate drying gets the Radix Ginseng active component;
The preparation method of Herba Epimedii active component: take by weighing epimedium herb, add 65～75% ethanol extractions twice, extracting solution has reclaimed ethanol, adds to leave standstill centrifugally after 2～3 times of water gagings stir, and centrifugal liquid is crossed D
101Macroporous resin column absorption, the water eluting is resolved with 60～70% ethanol, and desorbed solution concentrates after drying, and dried cream adds 90～95% ethanol extraction, and the extracting solution concentrate drying gets the Herba Epimedii active component;
For the different Chinese medicine with blood circulation promoting and blood stasis dispelling function that is selected from Flos Carthami, Rhizoma Chuanxiong, Radix Notoginseng, Radix Puerariae, Herba Erigerontis, Radix Salviae Miltiorrhizae, Folium Ginkgo, the preparation method of their active component is as follows:
The preparation method of Flos Carthami active component: take by weighing Flos Carthami, add water temperature and soak secondary, warm macerating liquid is centrifugal, and centrifugal liquid is crossed non-polar macroporous resin, carries out eluting, and eluent is crossed detached dowel, gets the Flos Carthami active component;
The preparation method of Rhizoma Chuanxiong active component: take by weighing the Rhizoma Chuanxiong medical material, the AB-8 macroporous resin adsorption is crossed in the centrifugal while hot back of extracting in water twice, extracting solution, washes with water to remove assortedly, resolves desorbed solution concentrating under reduced pressure, dry Rhizoma Chuanxiong active component with 50～80% ethanol;
The preparation method of pseudo-ginseng activity composition: get Radix Notoginseng and add 65～75% ethanol extractions, decompression recycling ethanol, D is crossed in centrifugal back
101Macroporous resin column absorption washes with water and removes assortedly, resolves with 50～80% ethanol, and the desorbed solution concentrating under reduced pressure must the pseudo-ginseng activity composition after eluent concentrates;
The preparation method of Radix Puerariae active component: get Radix Puerariae and add 65～75% ethanol extractions twice, decompression recycling ethanol adds 2～5 times of water gagings and is heated to 45～65 ℃ of fully dissolvings to doing, filtered while hot, and 45～55 ℃ of conditions of filtrate were descended D
101Macroporous resin column absorption washes with water and removes assortedly, and reuse 60～70% ethanol are resolved, and desorbed solution is crossed polyamide column after having reclaimed ethanol, uses the Different concentrations of alcohol gradient elution, and recrystallization got the Radix Puerariae active component after eluent was concentrated;
The preparation method of Herba Erigerontis active component: it is an amount of to get Herba Erigerontis, with 75% ethanol merceration of 3～4 times of amounts three times, the cooling bath concentrating under reduced pressure becomes extractum, and heating aqueous alkali adjust pH is to about 6.5, filter while hot, filtrate adds sulphuric acid adjust pH to 2.0～2.5, leaves standstill 48 hours, separates out precipitate, the precipitate washing with alcohol, use recrystallizing methanol, the crystal vacuum drying is volatilized methanol, promptly get the Herba Erigerontis active component;
The preparation of Folium Ginkgo active component: take by weighing Folium Ginkgo, add 65～75% ethanol extraction secondary, extracting solution has reclaimed ethanol, adds to leave standstill centrifugally after water stirs, and centrifugal liquid is crossed DM
130Macroporous resin, the water eluting, reuse ethanol is resolved, and desorbed solution concentrates the back spray drying, and dried cream adds the height ethanol extraction, and the extracting solution concentrate drying gets dry extract; And adopt extraction to remove the harmful substance ginkgoic acid to get ginkgetin and total lactone;
The preparation of Radix Salviae Miltiorrhizae active component: take by weighing Radix Salviae Miltiorrhizae, adding water temperature soaks, warm macerating liquid is centrifugal, centrifugal liquid is evaporated to 1/5 of original volume, adds acid and transfers PH to 2～3, the ethyl acetate extraction of usefulness equivalent 2 times, acetic acid ethyl fluid is evaporated on a small quantity, leave standstill and take off a layer slag, lower floor's slag recrystallization gets the salvianolic acid constituents;
Get above Herba Epimedii active component, the Radix Ginseng active component according to different prescriptions, with the extraction active component proportioning prescription that is selected from Flos Carthami, Rhizoma Chuanxiong, Radix Notoginseng, Radix Puerariae, Herba Erigerontis, Radix Salviae Miltiorrhizae, Folium Ginkgo, is made preparation according to the galenic pharmacy routine techniques.
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| CN 200510070628 CN1686469A (en) | 2005-05-18 | 2005-05-18 | Medicament for treating coronary heart disease and its preparation method |
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| US7906159B2 (en) | 2008-04-17 | 2011-03-15 | Vivien Chou | Herbal compositions and methods for enhancing vital energy and athletic performance |
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| CN102961517A (en) * | 2012-10-31 | 2013-03-13 | 成都医路康医学技术服务有限公司 | Pharmaceutical composition for treating cardiovascular and cerebrovascular disease |
| CN104840863A (en) * | 2015-05-27 | 2015-08-19 | 大连大学 | Traditional Chinese medicine composition for treating myocardial ischemia as well as preparation method and application thereof |
| CN108014173A (en) * | 2018-02-02 | 2018-05-11 | 张道明 | A kind of external-applied ointment for treating coronary heart disease |
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- 2005-05-18 CN CN 200510070628 patent/CN1686469A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7906159B2 (en) | 2008-04-17 | 2011-03-15 | Vivien Chou | Herbal compositions and methods for enhancing vital energy and athletic performance |
| US8119170B2 (en) | 2008-04-17 | 2012-02-21 | Wen Hsien Chou | Herbal compositions and methods for enhancing vital energy and athletic performance |
| CN102188471A (en) * | 2011-04-20 | 2011-09-21 | 长春中医药大学 | Pharmaceutical composition for treating Alzheimer disease symptom and its preparation method |
| CN102961517A (en) * | 2012-10-31 | 2013-03-13 | 成都医路康医学技术服务有限公司 | Pharmaceutical composition for treating cardiovascular and cerebrovascular disease |
| CN104840863A (en) * | 2015-05-27 | 2015-08-19 | 大连大学 | Traditional Chinese medicine composition for treating myocardial ischemia as well as preparation method and application thereof |
| CN104840863B (en) * | 2015-05-27 | 2020-05-19 | 南方医科大学 | Traditional Chinese medicine composition for treating myocardial ischemia and preparation method and application thereof |
| CN108014173A (en) * | 2018-02-02 | 2018-05-11 | 张道明 | A kind of external-applied ointment for treating coronary heart disease |
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