CN1680600A - Polymorphic point of stearyl cozymase A desaturase gene mononucleotide for identifying behavior for back fat thickness of pig and use thereof - Google Patents
Polymorphic point of stearyl cozymase A desaturase gene mononucleotide for identifying behavior for back fat thickness of pig and use thereof Download PDFInfo
- Publication number
- CN1680600A CN1680600A CNA200510023930XA CN200510023930A CN1680600A CN 1680600 A CN1680600 A CN 1680600A CN A200510023930X A CNA200510023930X A CN A200510023930XA CN 200510023930 A CN200510023930 A CN 200510023930A CN 1680600 A CN1680600 A CN 1680600A
- Authority
- CN
- China
- Prior art keywords
- pig
- gene
- scd
- stearyl
- proterties
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A single nucleotide polymorphic locus of stearamide CoA affecting obviously backfat thickness. It selects PCA library firstly, then colons SCD gene, separates mRNA of SCD gene and identifies SNP C3101T of exon2. Finally, decides type of SCN C3101T gene by way of PCR-RFLP.
Description
Technical field
The present invention relates to the stearyl-coenzyme A desaturase gene mononucleotide polymorphic site differentiating remarkably influenced fat thickness at back of pig proterties, can be used for kind of swine improvement.
Background technology
Stearyl-coenzyme A desaturase (Stearoyl-CoA desaturase, SCD) be the rate-limiting enzyme (rate-limiting enzyme) of synthetic monounsaturated fatty acids, it is arranged in endoplasmic reticulum, main catalysis saturability lipid acid one stearoyl-CoA (C16:0) and palmitoyl coenzyme A (C18:0) import hydrogen bond between the 9th and 10 carbon atoms, form monounsaturated fatty acids-oleic acid (16:1) and palmitinic acid (C18:1) respectively.
Because the fluidity (membrane fluidity) of cytolemma in the ratio remarkably influenced of saturability lipid acid and nonsaturation lipid acid in the cell, and then influence signal conduction, thereby in people and mouse the change and the disease close association such as hypertension, nervous system disease, immune disorder and cancer of SCD enzymic activity.In addition, SCD effect gene bluntness.SCD Gene Deletion mouse (SCD-/-) although increased under high-energy diet condition searches for food, and the deposition of body fat significantly reduces.Leptin (leptin) absence type mouse (ob/ob) shows extremely fat phenotype, and with ob/ob mouse and SCD-/-mouse hybridization, the fat phenotype disappearance of the SCD of formation and thin voxel Leptin Gene Double disappearance mouse (ob/ob, SCD-/-).It is important component in the Leptin signals-modulating path that Cohen etc. disclose SCD by the gene expression chip technology, Leptin by preventing the SCD expression of gene, has promoted the energy metabolism of body when suppressing the animal feed, and then make and lose weight, body lipidosis reduces.When the SCD expression of gene was prevented, the content of saturability lipid acid rose in the born of the same parents, and (Acetyl-CoA carboxylase, activity ACC) stop the formation of malonyl coenzyme A (Malonyl CoA) to suppress hexanoyl coenzyme A carboxylase.Malonyl coenzyme A is preventing the intercellular free fatty acids to transport to plastosome, carries out oxidizing reaction.Thereby the SCD enzymic activity descends and can promote oxidation of fatty acids to decompose.These studies show that: SCD is the lipometabolic a kind of key enzymes of regulation and control, and the SCD gene is one of desirable candidate gene of research Mammals fatty deposits proterties.
In recent years, the SCD gene was positioned, separated and differentiated in Mammalss such as mouse, people, goat, sheep, ox in succession.In mouse, clone five kinds of SCD genes at present, had tissue expression specificity.The SCD functional gene is positioned on No. 10 karyomit(e)s of people, has a pseudogene on No. 17 karyomit(e)s.The SCD assignment of genes gene mapping of sheep and goat is in 22q21.Taniguchi etc. differentiate 3 SNPs sites in ox SCD gene open reading frame (ORF), one of them missense mutation causes coded amino acid to sport L-Ala by Xie Ansuan.The brown ox of Japan (Japanese Black Cattle) shows as two class haplotypes (A and Type B) on these three sites, wherein the monounsaturated fatty acids content of the individual intramuscular fat of A type is significantly higher than the Type B individuality, and fusing point but is lower than Type B individuality (P＜0.001)
[5]The applicant carried out pig SCD gene Fine Mapping, separation, clone, SNP polymorphic site discriminating and influence the research of fatty deposits proterties, the means of new molecular breeding are provided in the hope of the seed selection improvement for kind of label of pig fat deposition description proterties.
Summary of the invention
First purpose of the present invention provides a stearyl-coenzyme A desaturase gene mononucleotide polymorphic site of differentiating remarkably influenced fat thickness at back of pig proterties, detect this polymorphic site by modern molecular biology technique, utilize marker assisted selection (MAS) to select favourable aflelotype unity to reserve seed for planting, can significantly improve population thickness of backfat proterties, improve the lean meat output capacity.
Second purpose of the present invention is the application of stearyl-coenzyme A desaturase gene mononucleotide polymorphic site in seed selection improvement boar.
First purpose of the present invention is achieved in that
(1), bacterial artificial chromosome (PAC) library screening: the applicant according to the homology zone of people, sheep SCD gene and pig EST (expressed sequence tag) sequences Design primer to 1 (F:5 '-ATC CGA GAG CCA AGATGC C-3 '; R:5 '-ATG GTA GTG GTG GTT GTG TAG-3 '), amplification contains 591bp DNA (thymus nucleic acid) fragment of complete first intron.Behind dna sequencing, design pig SCD gene-specific primer once more to 2 (5 '-CAC AAC TAC CAC CAC ACC-3 '; R:5 '-GTC ATA AGC CAG ACC CAG-3 '), utilize this, the single PAC positive colony (TAIGP714K1477) that obtain to contain total length SCD gene, inserts sheet segment length 100kb to primer screening pig PAC genome dna library;
(2), the clone of pig total length SCD gene: positive pac clone DNA is through AccI, BamHI, HindIII, KpnI, PntI, behind SacI and the XbaI restriction enzyme digestion, with primer 1 and 2 amplified production is hybridized, choose the restriction fragment that hybridization signal is arranged, be cloned into the pGEM-4Z carrier, adopt contig (contig) to make up and the subclone sequencing technologies, obtained the SCD full length gene dna sequence dna (GenBank sequence number .AY487830) of 20985bp;
(3), the separation of pig total length SCD gene mRNA (messenger RNA(mRNA)): utilize Trizaol reagent to extract liver total RNA, adopt Super SMART
TMPCR cDNA Synthesis Kit test kit synthesizes full-length cDNA.By 5 ' RACE (Rapid Amplification of cDNA End, rapid amplifying cDNA end) and 3 ' RACE technology, the cDNA sequence (GenBank sequence number .AY487830) of having separated pig SCD full length gene 5034bp has been differentiated the detailed structure of pig SCD gene thus.The primer of 5 ' RACE is: 5 '-ATG GCGTAA CGA AGA AAG GTG-3 '; The primer of 3 ' RACE is: Outer primer, 5 '-CGA TGT CAAGGT TAC ACG GGT GG-3 '; Inner primer, 5 '-GTG CCA CTG ACT TGC TAT GTG ACC-3 ';
(4), the discriminating of SNP C3101T on the exon 2: according to the full length DNA sequence of pig SCD gene, the design primer to 3 (F:5 '-GCA CTT CCA CTC TCT TTC-3 '; R:5 '-GCT GGT GTT TCT ACTCTG-3 '), this 395bp amplified fragments to primer comprises whole exon 2.20 first ancestor's individualities (2 white Du Luoke individualities with Chinese painted face in Beijing opera pig * white duroc resource family colony, 18 painted face in Beijing opera individualities) DNA is a template, utilize primer to carry out pcr amplification to 3, behind the PCR product purification, directly order-checking, the sequencing result that compares Different Individual, differentiate at No. 3101 Nucleotide (if according to SCD gene open reading frame, count from initiator codon, then be No. 641 Nucleotide) polymorphism that sports thymus pyrimidine (T) by cytosine(Cyt) (C) of existence, be SNP C3101T site, this site is:
1????tctagaccct?ttgaatttcc?acttaaattt?tagaatctgc?ttgtctattt?cctcaaagaa
61???atctgttgga?tttctatagt?gattaaatta?atctatagat?caacactgag?tctgtccatt
121??tatgaacaca?gaatataaca?aattcttttt?ttctctctat?ttaagagttt?taaagttttt
181??ctcagcattg?ttttgatact?ttcagtgtag?ctatcctata?aacatttaga?tttattcttt
241??gtgacatttt?tgatgttctt?ttaatttttg?tttttttagg?gccgcacctg?tggcacatga
381??agttcctggg?ccaggggtcg?aatcagagcc?aaagctacca?gcctatacca?ccaccatagc
361??aacgccaggt?ctggaccgca?tctgtgatgt?acgccggagc?tcgtgacaat?gccagatcct
421??taacccactg?agcaaggcca?gcatgtaacc?cacaacctcg?tggacactag?tcgagttctt
481??aacccgctga?gccacaagga?gaactccatt?tttgatgcta?ttttaaacag?tatattttaa
541??atttcattct?gcaattgttt?tttaatagaa?tataggacag?aataattttt?ttatactggt
601??ttctatcttg?acctttctaa?attcacttac?caattttgac?acttttttgg?tggtagattc
661??tatacatttt?tcatacacac?agttaggtct?tctgcaaata?ataacagttt?ctttcttctt
721??ttcctatctt?tatatctttc?atttcttttt?tcctgtccat?catgcaacct?aggatttcta
781??gcacaatgtt?gactagaaat?ggtagacgat?tatatgcttg?cctgttctct?taaggggaaa
841??tttttttttt?ttttttttgg?tctttttaag?cctgcaccca?tggctaggaa?ctgtagctgc
901??tggcctatgc?cacagccaca?gcaacactgg?atccgaactg?tatctgtgac?ctacaccaca
961??gctcatggct?atgccagatc?cttaacccac?tgagcgaggc?cagggatcga?accctcgacc
1021?tcacggttcc?tagtcggatt?cgtctccgct?gcgccacaac?gggaactccc?acgactaatg
1081?attaagtaaa?atgattcttg?tggactgact?tgcctaataa?ttctatagct?gatagctatt
1141?tttgtcccac?ttacaagtcc?ttttgcctgc?ttctggatga?aggcacacgc?tgcgcttcct
1201?cccttttctc?atgaaagcca?ttccaaaatt?acaactcgac?aatctgaaga?atttccaaaa
1261?catcccgcac?acgttcgggg?tggggggggg?gcgtgggcac?aaggacggga?tcgctcactg
1321?gtgcctggcg?gcgaacgtcc?cctgctgggc?ttgcctagta?gctgaacgcc?gtgcgcgcgt
1381?gcaggtccac?acatctatct??atgcatctac??gtgtgtgtac??acggctgccg?gcctggagac
1441?cggcgagttc?agcagtacgc?atctatcgcc?ccgccgccag?tgggcggcgc?agagccattg
1501?ttccgccgcc?ggccgaccct?cagcccctgc?ggcacgcgcg?ttcgtcacgg?agggaggtgg
1561?ggcttcgccg?ggcccccggc?ccccaacccg?gggtttgaag?gcacgtctcc?ccctcccacg
1621?cgtctccgcg?agctagaagg?cgggagtgga?gttgtgcgta?gcggtcgccc?gcgcggcttc
1681?acccacactt?tccccggaaa?cttccctagt?gcccatcctt?tcgcgaagtg?ccccagggcc
1741?agtcctgggc?tagcacccac?ctcccacccc?cctactgagt?cctcttcctc?ttccagggaa
1801?gacgctctcg?agcgcctccg?aggaggcagg?acgccgggca?gaagcccagc?ggccggtgga
1861?agagaagctg?aggaggagaa?agggagggga?gggggagtga?ggagctcgcg?gcagagggaa
1921?cagcagattg?cgccgagcca?atggcaacgg?caggacgagg?tggcaccaaa?ttcccttcgg
1981?ccaatgacgc?gccagagtct?acagaagccc?attagcattt?ccccaggggc?aggggcagag
2041?gcaggggctg?cggagaccga?gccgcggagt?gtctgcagca?tccagttttt?gcgtctccgg
2101?cccccagcaa?gccccggctc?tctgtctcct?cccctctccc?gcccatgcgg?ttctcccacg
2161?gtgagccaac?tctgcgcact?ttgccccttc?ttggcagcga?ataaaagggg?tcagaggaaa
2221?tacaggatac?ggtcgcccac?tgccagctct?agcctttaaa?tacccagcct?ggggagaccc
2281?acgcacagca?ggtcgggtgc?agacaccgac?ccaacgtgca?gcaaagggtt?ccgagcgcag
2341?catcgctgat?ctccgcgcaa?gagccggctc?cagcactagt?ctacgctcag?tgcggtctgc
2401?cacgaactcc?tccctgaagt?ctcatccctg?ggaaagtgat?cccaacgtcc?gagagcccag
2461?atgccggccc?acttgctgca?agaggaggtg?agtttccaag?taatggcccc?cagaccgcgg
2521?gctcgccagt?gctggttggg?cttctaggtg?actcggtgta?gaagagagtt?gagatcgccg
2581?aggacagcca?cctttttcga?gttgtatagt?cttcagtttg?ttgggaatgt?ggattgtaat
2641?ttgggaactt?agttctccaa?cttttgtttc?ctaaagcttc?aaagaaaaat?ctggtggtac
2701?ctgtaccctg?atgtgtgtag?gcatgcgggc?tttcctttgt?gtgtttgaag?gggtgcgagt
2761?gttttacccc?tctatttccc?cttcccctga?ggtttcaccc?tcctccccaa?tttctccgca
2821?cagttcctgg?ggacttgcgt?ctcttccatc?taggatagac?cttgccctca?ggtaacctgg
2881?gtattggggc?caggtctgtg?cctatacccc?gcccttcccc?cagctagacc?agggagtgaa
2941?gtggccccta?gtgtctccac?gtgtcctccc?ctctctccca?gctctgcact?tccactctct
3001?ttcagatctc?tagctcctac?acaaccacca?ctaccatcac?agcaccttcc?tccagggtcc
3061?tgcagaatgg?agggggcaag?ttggagaaga?ctccccaata?cgtggaagaa?gacatccgcc
3121?ctgaaatgaa?agatgacatc?tatgacccaa?cctaccagga?taaggagggc?ccaaggccca
3181?agcttgaata?tgtttggaga?aacatcatcc?tcatgagtct?gctacacttg?ggagccctgt
3241?atgggatcat?attgatcccc?acctgcaaga?tatacacctt?gctctggggt?aagaggtccc
3301?cttgtcctcc?tgtctcagta?tcccaggttc?tctccactct?tttaatacag?tagcaactta
3361?cacagagtag?aaacaccagc?ccctccaggc?cgaggttttt?ccaaccattt?ctcttaggtg
3421?ttgtggtggg?ctgggaaggg?agtctagggg?gtttgggatg?caggagtgtc?gattctgggg
3481?cctttaattt?gggggggggg?ttggagaggg?agccctggga?ccatgggcca?caggctttga
3541?agtgagtaga?tctgtgccag?ggagtcagct?tttctgtttc?aagtcatttt?cttgactgct
3601?tgtcccagcc?ctgccagcct?agaaagaatc?aaggccctta?cctggatgca?tgggaacatg
3661?gggctagact?agtgtgactt?tagagggcac?atgctgggta?tagtcacggg?gtagcaagag
3721?aaactactca?ggaagagggt?taagcagaag?aggggggaag?agagctgtgg?tcatccatga
3781?aattattttg?ccctgatcct?tagccttatc?aggctcctta?attacacaga?cagaggaagg
3841?cggctcctgc?ataactcatg?ttacatggat?gtcttgctct?gtgactccac?gtgggtctcc
3901?agggaactgc?acatgccact?caagaaaatg?ttctaatgaa?ataaggttaa?agaaacgttt
3961?cctgccccat?tccccaccca?tcctgctcca?gcccctctcc?cctgatgcca?ggagtgcctc；
(5), SNP C3101T declare type: this polymorphic site has changed the restriction enzyme site of Ta/iI restriction enzyme, can adopt PCR-TaiI-RFLP (Restriction fragment length polymorphism, RFLP restriction fragment length polymorphism) method to judge the genotype in this site.Utilize primer to 3 amplification genes of individuals group DNA, comprise the 50ng genomic dna in 25 μ l PCR (polymerase chain reaction) reaction systems, and 1 * PCR buffer (damping fluid) (QIAGEN, Hilden, Germany), 1.5mM MgCl
2, 100 μ MofdNTP (dideoxy nucleotide), 10pmol primer, 1.5 unit archaeal dna polymerases (Taq enzyme).Loop parameter is: 95 ℃, and 5min; 92 ℃, 30s, 54 ℃, 30s, 72 ℃, 30s; 35 circulations, last 72 ℃ are extended 10min.Utilize the Ta/iI enzyme to cut the PCR product, 25 μ l endonuclease reaction systems contain 15 μ lPCR products, 2 PsyI of unit enzymes and supporting 1 * buffer (MBI, Shanghai, China), 65 ℃ of incubations 3 hours, through 1.5% agarose gel electrophoresis separate, ethidium bromide staining takes pictures and keeps on file.The TT type is the 395bp dna fragmentation; The CC type is 280 and the 115bpDNA fragment, and the TC heterozygote then shows as 395,280 and three dna fragmentations of 115bp.
Second purpose of the present invention is achieved in that the present invention utilizes this polymorphic site and flanking DNA sequence information, adopts molecular biotechnology to differentiate idiotype, at fatty deposits proterties seed selection improvement boar.
The present invention has differentiated stearyl-coenzyme A desaturase (SCD) gene mononucleotide polymorphism (SNP) site that influences the fat thickness at back of pig proterties, detect this polymorphic site by modern molecular biology technique, utilize marker assisted selection (MAS) to select favourable aflelotype unity to reserve seed for planting, can significantly improve boar colony thickness of backfat proterties, improve the lean meat output capacity.
Description of drawings
Fig. 1 declares type figure for the PCR-TaiI-RFLP in the pig SCD gene SNP C3101T site that the present invention differentiates, wherein 2,3,5,6,7,9,10,11,12, No. 13 individualities are the CC type, and No. 14 individualities are the TT type, and 1,4,8 individualities are the TC type.
Embodiment
Below in conjunction with embodiment and contrast accompanying drawing the present invention is described in further detail.
Select boar core group individuality, adopt above-mentioned PCR-TaiI-RFLP method to differentiate the genotype in SCD gene SNP C3101T site, select favourable aflelotype unity to reserve seed for planting, the fatty deposits performance of improvement population after colony's subculture seed selection.
Embodiment:
Utilize self make up, with white Du Luoke and Chinese painted face in Beijing opera pig for parent for generations, to be used for the localized pig resource of functional gene family be laboratory animal.The wean in individual 46 days of 132 the resource family s-generation (F2 generation) boars after the castrating, is supported to 120 days on the child care hurdle, and diets of different nutrition level is 21% crude protein, 3300 KJ digestible energies, 1.25% Methionin; Pass on then and measure the station, every hurdle closes 10-12 pig, adopts the cafeteria feeding mode, and diets of different nutrition level is 16% crude protein, 3100KJ digestible energy, 0.78% Methionin.(ACEMO, Pontivy Cedex France) measure proterties such as record daily ingestion amount, feed conversion rate to adopt ACEMA64 electronics feeding automatic recognition system.When 240 ages in days, will measure individuality and butcher, 4 thickness of backfats at the shoulder when measuring record 240 age in days body weight, 6-7 rib, last rib and hip joint place, stomach fat is heavy, and leaf fat is heavy, and caul-fat heavily waits the fatty deposits proterties.
Adopting above-mentioned PCR-TaiI-RFLP method that 132 F2 individualities are carried out SNP C3101T genotype judges, (version 8.2 to adopt the SAS software package, SAS Inc., 2001) FREQ program, the heritable variation that the GLM statistical model is analyzed this site and the dependency of fatty deposits proterties.Be that fixed effect, carcass weight are concomitant variable with the genotype in the statistical model.Statistics is as shown in table 1, therefrom as seen, this site and thickness of backfat proterties significant correlation, CC type and TC type individuality are at the 6-7 rib thickness of backfat, the last rib thickness of backfat and 4 equal fat thickness significant differences (P＜0.05), the back fat of CC type individuality is thinner, is the required genotype individuality of seed selection improvement of bacon hogs.In commercial boar core group, subculture seed selection CC type individuality can further be improved the thickness of backfat proterties of population, improves the lean meat output capacity.
The correlation analysis of table 1SCD SNP C3101T genotype and white Du Luoke * painted face in Beijing opera pig F2 colony fatty deposits proterties
| Genotype | Quantity | Leaf fat heavy (gram) | Caul-fat heavy (gram) | Abdomen fat heavy (gram) | The thickness of backfat a????(cm) | The thickness of backfat b????(cm) | The thickness of backfat c????(cm) | The thickness of backfat d????(cm) | The thickness of backfat e????(cm) |
| CC (the CT of X ± SD) (the t value P value (%) of X ± SD) | ??80 ??39 | ??3551.3±107.38 ??3366.5±154.10 ??0.9815 ??0.3284 ??0.1824 | ????1718.4±45.55 ????1703.6±65.37 ????0.1847 ????0.8538 ????0.0324 | ????1778.8±32.79 ????1796.1±47.06 ????-0.3026 ????0.7628 ????-0.0451 | ????4.94±0.093 ????5.22±0.133 ????-1.7511 ????0.0826 ????-0.3428 | ????3.79±0.104 ????4.23±0.149 ????-2.3967 ????0.0181 ????-0.4722 | ????3.01±0.088 ????3.40±0.126 ????-2.5342 ????0.0126 ????-0.4943 | ????3.13±0.096 ????3.39±0.138 ????-1.5410 ????0.1261 ????-0.3044 | ????3.717±0.086 ????4.060±0.123 ????-2.2814 ????0.0244 ????-0.4683 |
P＜0.05 usefulness runics are represented; Subscript a, b, the thickness of backfat and this average thickness of backfat of 4 at the shoulder when c, d, e represent 240 age in days body weight respectively, 6-7 rib, last rib, hip joint place.
Claims (2)
1, stearyl-coenzyme A desaturase gene mononucleotide polymorphic site of differentiating remarkably influenced fat thickness at back of pig proterties, it is characterized in that: this polymorphic site sequence is as follows:
1?tctagaccct?ttgaatttcc?acttaaattt?tagaatctgc?ttgtctattt?cctcaaagaa
61?atctgttgga?tttctatagt?gattaaatta?atctatagat?caacactgag?tctgtccatt
121?tatgaacaca?gaatataaca?aattcttttt?ttctctctat?ttaagagttt?taaagttttt
181?ctcagcattg?ttttgatact?ttcagtgtag?ctatcctata?aacatttaga?tttattcttt
241?gtgacatttt?tgatgttctt?ttaatttttg?tttttttagg?gccgcacctg?tggcacatga
301?agttcctggg?ccaggggtcg?aatcagagcc?aaagctacca?gcctatacca?ccaccatagc
361?aacgccaggt?ctggaccgca?tctgtgatgt?acgccggagc?tcgtgacaat?gccagatcct
421?taacccactg?agcaaggcca?gcatgtaacc?cacaacctcg?tggacactag?tcgagttctt
481?aacccgctga?gccacaagga?gaactccatt?tttgatgcta?ttttaaacag?tatattttaa
541?atttcattct?gcaattgttt?tttaatagaa?tataggacag?aataattttt?ttatactggt
601?ttctatcttg?acctttctaa?attcacttac?caattttgac?acttttttgg?tggtagattc
661?tatacatttt?tcatacacac?agttaggtct?tctgcaaata?ataacagttt?ctttcttctt
721?ttcctatctt?tatatctttc?atttcttttt?tcctgtccat?catgcaacct?aggatttcta
781?gcacaatgtt?gactagaaat?ggtagacgat?tatatgcttg?cctgttctct?taaggggaaa
841?tttttttttt?ttttttttgg?tctttttaag?cctgcaccca?tggctaggaa?ctgtagctgc
901?tggcctatgc?cacagccaca?gcaacactgg?atccgaactg?tatctgtgac?ctacaccaca
961?gctcatggct?atgccagatc?cttaacccac?tgagcgaggc?cagggatcga?accctcgacc
1021?tcacggttcc?tagtcggatt?cgtctccgct?gcgccacaac?gggaactccc?acgactaatg
1081?attaagtaaa?atgattcttg?tggactgact?tgcctaataa?ttctatagct?gatagctatt
1141?tttgtcccac?ttacaagtcc?ttttgcctgc?ttctggatga?aggcacacgc?tgcgcttcct
1201?cccttttctc?atgaaagcca?ttccaaaatt?acaactcgac?aatctgaaga?atttccaaaa
1261?catcccgcac?acgttcgggg?tggggggggg?gcgtgggcac?aaggacggga?tcgctcactg
1321?gtgcctggcg?gcgaacgtcc?cctgctgggc?ttgcctagta?gctgaacgcc?gtgcgcgcgt
1381?gcaggtccac?acatctatct?atgcatctac?gtgtgtgtac?acggctgccg?gcctggagac
1441?cggcgagttc?agcagtacgc?atctatcgcc?ccgccgccag?tgggcggcgc?agagccattg
1501?ttccgccgcc?ggccgaccct?cagcccctgc?ggcacgcgcg?ttcgtcacgg?agggaggtgg
1561?ggcttcgccg?ggcccccggc?ccccaacccg?gggtttgaag?gcacgtctcc?ccctcccacg
1621?cgtctccgcg?agctagaagg?cgggagtgga?gttgtgcgta?gcggtcgccc?gcgcggcttc
1681?acccacactt?tccccggaaa?cttccctagt?gcccatcctt?tcgcgaagtg?ccccagggcc
1741?agtcctgggc?tagcacccac?ctcccacccc?cctactgagt?cctcttcctc?ttccagggaa
1801?gacgctctcg?agcgcctccg?aggaggcagg?acgccgggca?gaagcccagc?ggccggtgga
1861?agagaagctg?aggaggagaa?agggagggga?gggggagtga?ggagctcgcg?gcagagggaa
1921?cagcagattg?cgccgagcca?atggcaacgg?caggacgagg?tggcaccaaa?ttcccttcgg
1981?ccaatgacgc?gccagagtct?acagaagccc?attagcattt?ccccaggggc?aggggcagag
2041?gcaggggctg?cggagaccga?gccgcggagt?gtctgcagca?tccagttttt?gcgtctccgg
2101?cccccagcaa?gccccggctc?tctgtctcct?cccctctccc?gcccatgcgg?ttctcccacg
2161?gtgagccaac?tctgcgcact?ttgccccttc?ttggcagcga?ataaaagggg?tcagaggaaa
2221?tacaggatac?ggtcgcccac?tgccagctct?agcctttaaa?tacccagcct?ggggagaccc
2281?acgcacagca?ggtcgggtgc?agacaccgac?ccaacgtgca?gcaaagggtt?ccgagcgcag
2341?catcgctgat?ctccgcgcaa?gagccggctc?cagcactagt?ctacgctcag?tgcggtctgc
2401?cacgaactcc?tccctgaagt?ctcatccctg?ggaaagtgat?cccaacgtcc?gagagcccag
2461?atgccggccc?acttgctgca?agaggaggtg?agtttccaag?taatggcccc?cagaccgcgg
2521?gctcgccagt?gctggttggg?cttctaggtg?actcggtgta?gaagagagtt?gagatcgccg
2581?aggacagcca?cctttttcga?gttgtatagt?cttcagtttg?ttgggaatgt?ggattgtaat
2641?ttgggaactt?agttctccaa?cttttgtttc?ctaaagcttc?aaagaaaaat?ctggtggtac
2701?ctgtaccctg?atgtgtgtag?gcatgcgggc?tttcctttgt?gtgtttgaag?gggtgcgagt
2761?gttttacccc?tctatttccc?cttcccctga?ggtttcaccc?tcctccccaa?tttctccgca
2821?cagttcctgg?ggacttgcgt?ctcttccatc?taggatagac?cttgccctca?ggtaacctgg
2881?gtattggggc?caggtctgtg?cctatacccc?gcccttcccc?cagctagacc?agggagtgaa
2941?gtggccccta?gtgtctccac?gtgtcctccc?ctctctccca?gctctgcact?tccactctct
3001?ttcagatctc?tagctcctac?acaaccacca?ctaccatcac?agcaccttcc?tccagggtcc
3061?tgcagaatgg?agggggcaag?ttggagaaga?ctccccaata?cgtggaagaa?gacatccgcc
3121?ctgaaatgaa?agatgacatc?tatgacccaa?cctaccagga?taaggagggc?ccaaggccca
3181?agcttgaata?tgtttggaga?aacatcatcc?tcatgagtct?gctacacttg?ggagccctgt
3241?atgggatcat?attgatcccc?acctgcaaga?tatacacctt?gctctggggt?aagaggtccc
3301?cttgtcctcc?tgtctcagta?tcccaggttc?tctccactct?tttaatacag?tagcaactta
3361?cacagagtag?aaacaccagc?ccctccaggc?cgaggttttt?ccaaccattt?ctcttaggtg
3421?ttgtggtggg?ctgggaaggg?agtctagggg?gtttgggatg?caggagtgtc?gattctgggg
3481?cctttaattt?gggggggggg?ttggagaggg?agccctggga?ccatgggcca?caggctttga
3541?agtgagtaga?tctgtgccag?ggagtcagct?tttctgtttc?aagtcatttt?cttgactgct
3601?tgtcccagcc?ctgccagcct?agaaagaatc?aaggccctta?cctggatgca?tgggaacatg
3661?gggctagact?agtgtgactt?tagagggcac?atgctgggta?tagtcacggg?gtagcaagag
3721?aaactactca?ggaagagggt?taagcagaag?aggggggaag?agagctgtgg?tcatccatga
3781?aattattttg?ccctgatcct?tagccttatc?aggctcctta?attacacaga?cagaggaagg
3841?cggctcctgc?ataactcatg?ttacatggat?gtcttgctct?gtgactccac?gtgggtctcc
3901?agggaactgc?acatgccact?caagaaaatg?ttctaatgaa?ataaggttaa?agaaacgttt
3961?cctgccccat?tccccaccca?tcctgctcca?gcccctctcc?cctgatgcca?ggagtgcctc。
2, the application of stearyl-coenzyme A desaturase gene mononucleotide polymorphic site in fatty deposits proterties seed selection improvement boar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510023930XA CN1680600A (en) | 2005-02-05 | 2005-02-05 | Polymorphic point of stearyl cozymase A desaturase gene mononucleotide for identifying behavior for back fat thickness of pig and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510023930XA CN1680600A (en) | 2005-02-05 | 2005-02-05 | Polymorphic point of stearyl cozymase A desaturase gene mononucleotide for identifying behavior for back fat thickness of pig and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1680600A true CN1680600A (en) | 2005-10-12 |
Family
ID=35067347
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200510023930XA Pending CN1680600A (en) | 2005-02-05 | 2005-02-05 | Polymorphic point of stearyl cozymase A desaturase gene mononucleotide for identifying behavior for back fat thickness of pig and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1680600A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101230391B (en) * | 2007-11-07 | 2010-12-15 | 中国农业科学院北京畜牧兽医研究所 | Method for detecting pork quality traits |
| CN101942438A (en) * | 2010-09-10 | 2011-01-12 | 中国农业科学院北京畜牧兽医研究所 | Sheep back fat trait-related SNP and application thereof |
| CN101705288B (en) * | 2009-11-17 | 2011-12-28 | 西北农林科技大学 | Mononucleotide polymorphism of milk goat MFG-E8 genes and detection method thereof |
| CN101705290B (en) * | 2009-11-17 | 2011-12-28 | 西北农林科技大学 | Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof |
| CN103911373A (en) * | 2014-03-18 | 2014-07-09 | 江西农业大学 | Major SNP marker capable of affecting pork aliphatic acid and application of major SNP marker in breeding pigs meat quality heredity improvement |
| CN105200146A (en) * | 2015-10-27 | 2015-12-30 | 湖北省农业科学院畜牧兽医研究所 | SNP genetic marker correlated to pig fat deposition property and application |
| CN108949907A (en) * | 2018-07-11 | 2018-12-07 | 南京农业大学 | One kind SNP marker primer pair relevant to Suhuai pig intramuscular fat content and its application |
| CN110408708A (en) * | 2019-08-06 | 2019-11-05 | 山东省农业科学院畜牧兽医研究所 | A kind of method of multiple labeling assisted Selection pig intramuscular fat character |
-
2005
- 2005-02-05 CN CNA200510023930XA patent/CN1680600A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101230391B (en) * | 2007-11-07 | 2010-12-15 | 中国农业科学院北京畜牧兽医研究所 | Method for detecting pork quality traits |
| CN101705288B (en) * | 2009-11-17 | 2011-12-28 | 西北农林科技大学 | Mononucleotide polymorphism of milk goat MFG-E8 genes and detection method thereof |
| CN101705290B (en) * | 2009-11-17 | 2011-12-28 | 西北农林科技大学 | Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof |
| CN101942438A (en) * | 2010-09-10 | 2011-01-12 | 中国农业科学院北京畜牧兽医研究所 | Sheep back fat trait-related SNP and application thereof |
| CN103911373A (en) * | 2014-03-18 | 2014-07-09 | 江西农业大学 | Major SNP marker capable of affecting pork aliphatic acid and application of major SNP marker in breeding pigs meat quality heredity improvement |
| CN103911373B (en) * | 2014-03-18 | 2016-01-20 | 江西农业大学 | Affect the main effect SNP marker of pork fat acid constituents and the application in kind of pig flesh characters genetic improvement thereof |
| CN105200146A (en) * | 2015-10-27 | 2015-12-30 | 湖北省农业科学院畜牧兽医研究所 | SNP genetic marker correlated to pig fat deposition property and application |
| CN105200146B (en) * | 2015-10-27 | 2017-11-14 | 湖北省农业科学院畜牧兽医研究所 | The SNP genetic marker related to label of pig fat deposition description character and application |
| CN108949907A (en) * | 2018-07-11 | 2018-12-07 | 南京农业大学 | One kind SNP marker primer pair relevant to Suhuai pig intramuscular fat content and its application |
| CN110408708A (en) * | 2019-08-06 | 2019-11-05 | 山东省农业科学院畜牧兽医研究所 | A kind of method of multiple labeling assisted Selection pig intramuscular fat character |
| CN110408708B (en) * | 2019-08-06 | 2022-06-10 | 山东省农业科学院畜牧兽医研究所 | Method for multi-marker assisted selection of intramuscular fat character of pig |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1680600A (en) | Polymorphic point of stearyl cozymase A desaturase gene mononucleotide for identifying behavior for back fat thickness of pig and use thereof | |
| CN103898107B (en) | Major SNP (single nucleotide polymorphism) marker influencing growth traits of pigs and application thereof in genetic improvement of productivity of breeding pigs | |
| CN109811063B (en) | SNP molecular marker related to pig growth speed and application thereof | |
| CN111926086B (en) | Molecular marker influencing oblique growth of chicken body and application thereof | |
| CN112195253B (en) | SNP (Single nucleotide polymorphism) locus for increasing content of fatty acid C14:0 in chicken and method for breeding high-quality chicken strain by using SNP locus | |
| CN111926085B (en) | Molecular marker influencing chicken muscle brightness and application thereof | |
| CN109207609B (en) | SNP (Single nucleotide polymorphism) related to chicken growth traits and application thereof | |
| CN107034307B (en) | SNP marker related to growth traits of micropterus salmoides and application thereof | |
| KR100804310B1 (en) | 4 DNA marker of adipocyte-fatty acid binding protein gene related the intramuscular fat content in beef cattle | |
| Lyons et al. | Characterization of AFLP markers associated with growth in the Kuruma prawn, Marsupenaeus japonicus, and identification of a candidate gene | |
| CN113862375A (en) | SNP (Single nucleotide polymorphism) marker of AANAT (ananas site-directed translation) and ASMT (antisense-fluorescent marker) genes of cattle and application thereof | |
| CN103215258B (en) | Two major mark for increasing number of pig rib and application for pig breeding | |
| KR101472323B1 (en) | SNP marker for the prediction of fatty acid composition of intercross pig population | |
| CN104232630A (en) | Two major markers for increasing number of pork ribs and application thereof in pig breeding | |
| CN114921568B (en) | SNP molecular marker related to Qinchuan cattle body ruler and meat quality traits and application thereof | |
| CN111850136B (en) | Application of MRVI1 gene as marker for screening excellent meat quality traits of beef cattle | |
| CN116179714A (en) | Molecular marker related to chicken slaughtering and meat quality characteristics and breeding method of high-quality slaughtering and processing type novel variety | |
| CN111961732B (en) | Molecular marker influencing full bore weight of chicken and application thereof | |
| CN110938705B (en) | Molecular marker influencing early weight of goat and primer and application thereof | |
| CN113528675A (en) | Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and identification method and application thereof | |
| Luan et al. | Genome-wide association study for loin muscle area of commercial crossbred pigs | |
| CN106319063B (en) | A method of ox meat quality is detected using PRSS2 genetic marker | |
| CN1282338A (en) | A DNA molecule encoding mutant prepro-neuropeptide Y, a mutant signal peptide, and uses thereof | |
| Kashani et al. | Single nucleotide polymorphisms of the ovine ADRB3 gene in crossbred Australian sheep supplemented with Spirulina (Arthrospira platensis) cyanobacterial microalgae | |
| CN110982912B (en) | Method for detecting cattle carcass and meat quality traits by using RRAD genetic marker |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |