The startup method of anaerobic ammonia oxidation reactor
Technical field
The present invention relates to a kind of startup method of anaerobic ammonia oxidation reactor, start the method for anaerobic ammonia oxidation reactor with denitrification biofilm.
Background technology
Anammox is a kind of newfound with NH
4 +For electron donor, with NO
2 -Microbial reaction for electron acceptor(EA) generation nitrogen has the excellent development application prospect in the wastewater biological denitrificaion field.But as the anaerobic ammonia oxidizing bacteria poor growth of this technology main body, cause anaerobic ammonia oxidation reactor long start time, limited this The Application of Technology.
The cotton-shaped anaerobic sludge of many both at home and abroad employings directly starts anaerobic ammonia oxidation reactor as kind of mud at present, and start time is long.For floc sludge, microbial film can effectively be held and stay poky microorganism, improves the biomass in the reactor.Have low price, advantage (seeing reference 1 for details) that the attached microbial performance is good as the soft cellulose filler of one of biofilm carrier, but be not applied to anaerobic ammonia oxidation process so far as yet.
Reference:
1. open from outstanding. drainage system (volume two). Beijing: China Construction Industry Press, 1996 (the 3rd editions), 215-216.
2. gold snow is marked, Yan Ning, and three kinds of denitrification process are relatively. water supply and drainage, 2003,29 (1): 32-34.
3. Zhou Lixiang, Hu Aitang, Hu Zhongming. anaerobically digested sludge chemical constitution and environmental chemistry character thereof. plant nutrition and fertilizer journal, 1997,3 (2): 176-181.
4. Wang Yi, Peng Dangcong. denitrification granular sludge research. environmental science and technology, 2004,27 (1): 15-17,50.
Summary of the invention
The objective of the invention is provides a kind of anaerobic ammonia oxidation reactor startup method in order to overcome anaerobic ammonia oxidation reactor long deficiency start time.This method can not only be accelerated the startup of anaerobic ammonia oxidation reactor, and reactor is stable, the nitric efficiency height.
The step of method is as follows:
1) cultivation of denitrification biofilm:
In the upflowing biofilm reactor of interior extension soft cellulose filler (performance of close therewith upflowing elastic filler biofilm reactor see reference document 2), with municipal wastewater treatment plant anaerobically digested sludge or direct negate nitrifying sludge is kind of a mud (its basic proterties is referring to document 3-4), under 20~35 ℃ of temperature, carry out the cultivation of denitrification biofilm, after 1 month, on soft-filler, form the lark denitrification biofilm;
2) startup of anaerobic ammonia oxidation reactor:
Adopt NO
2 --N and NH
4 +The ratio of-N is 1~1.5 water inlet, and control water inlet pH is a neutrallty condition, is that secondary kind mud carries out the startup of anaerobic ammonia oxidation reactor with the denitrification biofilm, initial start stage water inlet NH
4 +-N and NO
2 --N concentration is controlled at 70~100mg/L, starts anaerobic ammonia oxidation reactor in 3 months, water outlet NH
4 +-N and NO
2 --N concentration all is lower than 1mg/L.After starting successfully, can progressively improve water inlet nitrogen load, the nitrogen concentration amplification of at every turn intaking is 35~70mg/L.
The invention has the beneficial effects as follows that denitrification mud wide material sources are easy to obtain, is that kind of mud can successfully start anaerobic ammonia oxidation reactor in 3 months time with denitrification biofilm, at this moment, and NH
4 +With NO
2 -Clearance is higher than 98% and 99%, and volumetric loading can reach 143mg TN/L.d, and can obtain the denitrification effect (maximum volume is removed load and reached 658mg TN/L.d) of stability and high efficiency.According to the anaerobic ammonia oxidation reactor device operating performance indication parameter that proposes, effectively the startup process of monitoring reaction device and in time adjustment operating parameter.
Embodiment
The present invention utilizes the fast characteristics of denitrifying bacteria growth, under the condition of temperature and water inlet pH neutral, is kind of a mud with anaerobically digested sludge earlier in normal pressure, cultivates denitrification biofilm in hanging with the reactor of soft-filler.Utilizing metabolism diversity and the matrix diversity of microorganism again, by changing water inlet matrix, is that secondary kind mud carries out the startup of anaerobic ammonia oxidation reactor with the denitrification biofilm, accelerates the purpose that anaerobic ammonia oxidation reactor starts to reach.Microbial film can effectively be held and stay microorganism, improves the biomass in the reactor.
The present invention proposes, in Anammox reaction startup and operational process, with NO
2 --N removal amount and NH
4 +Ratio between the-N removal amount, and the pH value in the reactor and mud change in color are as the indication parameter of anaerobic ammonia oxidation reactor operating performance.
The present invention adopts the working method of aforesaid method processing anaerobically digested sludge pressing filtering liquid as follows:
1, the cultivation of denitrification biofilm: in the upflowing biofilm reactor of interior extension soft-filler, with municipal wastewater treatment plant anaerobically digested sludge is that kind of a mud is (under the situation with good conditionsi, directly the negate nitrifying sludge is kind of a mud), at normal pressure, under the condition that temperature range is 20~35 ℃, carry out the cultivation of denitrification biofilm.Can on soft-filler, observe the formation of lark denitrification biofilm after 1 month.
2, the startup of anaerobic ammonia oxidation reactor: after denitrification biofilm is cultivated successfully, adopt NH
4 +-N and NO
2 -The ratio of-N is about 1 water inlet, and control water inlet pH is a neutrallty condition, is that secondary kind mud carries out the startup of anaerobic ammonia oxidation reactor with the denitrification biofilm.Initial start stage water inlet NH
4 +-N and NO
2 --N concentration is controlled at 70~100mg/L, can successfully start anaerobic ammonia oxidation reactor, water outlet NH in 3 months
4 +-N and NO
2 --N concentration all is lower than 1mg/L.Monitor NO simultaneously
2 --N removal amount and NH
4 +Ratio between the-N removal amount, and pH value and mud change in color in the reactor instruct the startup and the operation of reactor.Starting successful reactor can progressively improve the water inlet nitrogen concentration (each concentration increasing degree is 35~70mg/L), and maximum volume is removed load and reached 658mg TN/L.d.
Embodiment
This test is to carry out in the upflowing biofilm reactor that a volume is 2L.Earlier soft-filler is placed anaerobically digested sludge to leave standstill 1 day before the test with the absorption microorganism.The basic proterties of anaerobically digested sludge is: SS 32.06g/L, and VSS 17.88g/L, VSS/SS 55.77%.
1, the cultivation of denitrification biofilm: at normal pressure, under 30 ℃ the condition, the anaerobically digested sludge pressing filtering liquid of certain sewage work of the nitrated mistake of learning from else's experience carries out the cultivation of denitrification biofilm for water inlet.In order to provide denitrification required carbon source, add the former water of an amount of Sewage Plant and make COD and NO
3 -The ratio of-N is controlled at about 3.6.The water inlet NO at initial stage
3 -The about 140mg/L of-N concentration, control water outlet NO
3 -The about 2mg/L of-N concentration progressively improves the water inlet substrate concn, and the operation through 1 month can observe the formation of lark denitrification biofilm on soft-filler.
2, the startup of anaerobic ammonia oxidation reactor: after denitrification biofilm is cultivated successfully, at normal pressure, 30 ℃, under the condition of pH7~8, with anaerobically digested sludge pressing filtering liquid (NH
4 +-N and NO
2 -The ratio of-N is about 1) for water inlet, be that secondary kind mud carries out the startup of anaerobic ammonia oxidation reactor with the denitrification biofilm.Initial start stage makes NH with tap water dilution water inlet
4 +-N and NO
2 --N concentration is controlled at 70~100mg/L, can successfully start anaerobic ammonia oxidation reactor, water outlet NH in 85 days
4 +-N and NO
2 --N concentration all is lower than 1mg/L.Monitor NO simultaneously
2 --N removal amount and NH
4 +Ratio between the-N removal amount, and pH value and mud change in color in the reactor instruct the startup and the operation of reactor.In the start-up course, NO
2 --N removal amount and NH
4 +The ratio of-N removal amount progressively carries out the transition between 1.4~1.6 by instability, there is the process that is raise by low in water outlet pH value, final and water inlet pH value reaches unanimity, be between 7.6~7.8, the mud color progressively carries out the transition to brown by lark, finally go out to represent the redness of Anammox mud characteristic feature, reflected the progressively process of dominate in reactor of Anammox reaction.Starting successful reactor can progressively improve the water inlet nitrogen concentration (each concentration increasing degree is 35~70mg/L), and maximum volume is removed load and reached 658mg TN/L.d.