CN1663455A - Preparation method of buckwheat polypeptide nutritional drink - Google Patents
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Abstract
Description
技术领域technical field
本发明涉及一种将食用蛋白质加工成多肽饮料的方法,更具体地说是涉及一种以荞麦芽为原料制备荞麦多肽营养饮料的方法。The invention relates to a method for processing edible protein into a polypeptide beverage, in particular to a method for preparing buckwheat polypeptide nutritional beverage with buckwheat germ as raw material.
背景技术Background technique
荞麦的深加工在国内外尚属起步阶段,中国发明专利申请号01124055.5,名称为“一种酶解荞麦蛋白制品的制备方法和用途”公开了一种荞麦生物酶解技术。该发明运用复合蛋白酶,将荞麦蛋白酶切成人体极易吸收的小分子活性多肽(氨基酸链)和多种氨基酸(特别是8种人体必须而又不能合成的氨基酸)维生素纤维素和微量元素,可用于食品营养增强剂,药品、功能性食品,饲料添加剂(增强营养和吸收)、植物生长剂。该发明的特征在于将酶解后的荞麦溶液加热沸腾后,冷却、凝固过滤,制得含肽和氨基酸、维生素、微量元素的荞麦蛋白混合干粉制品。由于荞麦食品营养丰富,对糖尿病、高血脂疾病有一定的预防作用,所以开发更容易被人体吸收的荞麦多肽营养饮料非常必要。The deep processing of buckwheat is still in its infancy at home and abroad. China's invention patent application number 01124055.5, titled "Preparation method and application of an enzymatically hydrolyzed buckwheat protein product", discloses a biological enzymatic hydrolysis technology of buckwheat. The invention uses compound protease to cut buckwheat protease into small molecule active polypeptides (amino acid chains) that are easily absorbed by the human body and various amino acids (especially 8 kinds of amino acids that are necessary for the human body but cannot be synthesized), vitamins, cellulose, and trace elements. Used in food nutrition enhancers, pharmaceuticals, functional foods, feed additives (enhancing nutrition and absorption), plant growth agents. The invention is characterized in that the enzymatically hydrolyzed buckwheat solution is heated and boiled, cooled, coagulated and filtered to prepare a buckwheat protein mixed dry powder product containing peptides, amino acids, vitamins and trace elements. Because buckwheat food is rich in nutrition and has a certain preventive effect on diabetes and hyperlipidemia, it is very necessary to develop a buckwheat polypeptide nutritional drink that is more easily absorbed by the human body.
发明内容Contents of the invention
本发明所要解决的技术问题是提供一种以荞麦为原料的荞麦多肽营养饮料的制备方法。The technical problem to be solved by the present invention is to provide a preparation method of buckwheat polypeptide nutritional beverage using buckwheat as raw material.
本发明所采用的技术方案:一种荞麦多肽营养饮料的制备方法,包括下列步骤:The technical scheme adopted in the present invention: a preparation method of buckwheat polypeptide nutritional beverage, comprising the following steps:
a.清洗、浸泡a. to wash and soak
清洗荞麦种子并剔除其中不饱满的种子,在20℃-30℃的温度下将种子浸泡24-48h,待种子发出1.0-1.5cm的芽备用;Wash the buckwheat seeds and remove the seeds that are not full, soak the seeds at a temperature of 20°C-30°C for 24-48h, and wait for the seeds to sprout 1.0-1.5cm buds for later use;
b.发芽b. to germinate
捞出浸泡后的荞麦种子铺在盘中,在20℃-30℃温度、75%-85%湿度、培养72-80h,待荞麦芽长到2-3cm后每隔2-3小时喷洒一次水,待荞麦芽长到10-15cm芽的子叶呈微黄胚轴呈亮白色时终止发芽过程;Take out the soaked buckwheat seeds and spread them on a plate, cultivate them for 72-80 hours at a temperature of 20°C-30°C and a humidity of 75%-85%, and spray water every 2-3 hours after the buckwheat sprouts grow to 2-3cm , when the buckwheat bud grows to 10-15cm, the cotyledon of the bud is yellowish and the hypocotyl is bright white, and the germination process is terminated;
c.破壁c. Broken wall
上述发芽的荞麦种子经喷淋水脱壳后,在高速剪切处理机中进行胚芽细胞破壁和微细化处理粉碎为30-50μm细度的浆体;The above-mentioned germinated buckwheat seeds are dehulled by spraying water, and the germ cells are broken and micronized in a high-speed shearing processor and pulverized into a slurry with a fineness of 30-50 μm;
d.酶解d. Enzyme hydrolysis
在上述荞麦芽浆体中加入中性蛋白酶20-300U/g,于30-50℃酶解1-5h;Add neutral protease 20-300U/g to the above-mentioned buckwheat malt slurry, and enzymolyze it at 30-50°C for 1-5h;
e.配料e. Ingredients
上述荞麦芽浆体经酶解后加入重量百分比的蜂蜜1-5%、低聚糖浆1-3%、黄原胶0.005-0.02%、羧甲基纤维素钠0.01-0.1%、海藻酸钠0.01-0.2%和微量的胡萝卜素;After enzymolysis of the above buckwheat malt slurry, 1-5% by weight of honey, 1-3% of oligosaccharide syrup, 0.005-0.02% of xanthan gum, 0.01-0.1% of sodium carboxymethyl cellulose, and 0.01% of sodium alginate are added -0.2% and trace amounts of carotene;
f.均质f. Homogeneous
将上述混合后的料液加热至50-70℃,经高压均质机在15-40Mpa下均质化得到荞麦多肽营养饮料的产品原液。The above-mentioned mixed feed liquid is heated to 50-70° C., and homogenized by a high-pressure homogenizer at 15-40 MPa to obtain a product stock solution of buckwheat polypeptide nutritional beverage.
步骤a所述的清洗浸泡优选在25℃的温度下将种子浸泡24-48h。The cleaning and soaking described in step a is preferably soaking the seeds for 24-48 hours at a temperature of 25°C.
步骤c所述的破壁优选采用离心转速为2000-10000rpm的高速剪切处理机对发芽的荞麦种子进行胚芽细胞破壁和微细化处理。For the wall breaking described in step c, it is preferable to use a high-speed shear processor with a centrifugal speed of 2000-10000 rpm to carry out germ cell wall breaking and miniaturization treatment on the germinated buckwheat seeds.
步骤d所述的酶解优选在43℃的温度下进行。The enzymolysis described in step d is preferably carried out at a temperature of 43°C.
步骤f所述的均质优选将料液加热至60℃再经高压均质机在25Mpa下均质化。For the homogenization described in step f, it is preferable to heat the feed liquid to 60° C. and then homogenize it with a high-pressure homogenizer at 25 MPa.
均质后的产品原液经130℃、1-3sUHT灭菌后,在无菌条件下灌装入容器成为市售成品。After the homogenized product stock solution is sterilized at 130°C and 1-3sUHT, it is filled into containers under aseptic conditions to become a commercially available finished product.
均质后的产品原液灌装入容器后再经110-120℃灭菌15-45min,成为市售成品。The homogenized product stock solution is filled into a container and then sterilized at 110-120°C for 15-45min to become a commercially available finished product.
本发明的有益效果是:所述荞麦多肽营养饮料的制备方法将荞麦发芽且全胚芽细胞破壁经生物技术酶解成为含荞麦多肽、荞麦氨基酸的可溶性荞麦蛋白复合物后,经配料、均质、无菌灌装制成荞麦多肽营养饮料。该饮料具有诱人的荞麦芽清香味,其氨基酸、芦丁、矿物质含量高,营养成分活性增强。与一般的荞麦制品相比,由于经过发芽、破壁、酶解、均质等一系列工艺手段,将荞麦中的一些大分子难消化蛋白转化为分子量分布在10000Dalton以下的荞麦多肽、荞麦氨基酸的可溶性荞麦蛋白复合物,从而不仅容易被人体所吸收,而且消除了荞麦中的过敏原因子。本发明通过采用复合稳定剂使饮料组织状态均匀稳定,通过采用全胚芽细胞破壁技术并在配方中加入了低聚糖浆,饮料具有较多含量的膳食纤维有助于人体中益生菌的生长。常饮本发明制备的饮料可大大增强人体健康,提高人体免疫力。The beneficial effect of the present invention is: the preparation method of the buckwheat polypeptide nutritional drink germinates buckwheat and breaks the wall of the whole germ cells to form a soluble buckwheat protein complex containing buckwheat polypeptide and buckwheat amino acid through biotechnology enzymolysis, and then mixes and homogenizes 1. Aseptic filling to make buckwheat polypeptide nutritional beverage. The beverage has an attractive clear fragrance of buckwheat sprouts, high content of amino acids, rutin and minerals, and enhanced activity of nutritional components. Compared with common buckwheat products, some macromolecular indigestible proteins in buckwheat are converted into buckwheat polypeptides and buckwheat amino acids with a molecular weight distribution below 10,000 Dalton due to a series of technological means such as germination, wall breaking, enzymatic hydrolysis, and homogenization. Soluble buckwheat protein complex, which is not only easily absorbed by the body, but also eliminates allergenic factors in buckwheat. In the present invention, the composite stabilizer is used to make the tissue state of the beverage uniform and stable, and the whole germ cell wall breaking technology is adopted and the oligosaccharide syrup is added in the formula, so that the beverage has a large content of dietary fiber, which is conducive to the growth of probiotics in the human body. Often drinking the beverage prepared by the invention can greatly enhance human health and improve human immunity.
附图说明Description of drawings
图1是荞麦多肽营养饮料制备流程图。Fig. 1 is a flow chart of the preparation of buckwheat polypeptide nutritional beverage.
具体实施方式Detailed ways
本发明的进一步详细描述:如图1所示,本发明荞麦多肽营养饮料的制备方法如下:Further detailed description of the present invention: as shown in Figure 1, the preparation method of buckwheat polypeptide nutritional drink of the present invention is as follows:
清洗:荞麦种子(包括苦荞和甜荞)经清洗,以冲洗掉灰尘和剔除不饱满种子。Washing: Buckwheat seeds (both tartary and sweet buckwheat) are washed to rinse off dust and remove unfilled seeds.
浸麦:在20℃-30℃的恒温条件(25℃最适)浸泡24-48h,待芽长出头1.0-1.5厘米。Wheat soaking: Soak at a constant temperature of 20°C-30°C (25°C is the most suitable) for 24-48 hours, until the buds grow 1.0-1.5 cm from the head.
发芽:捞出浸后麦铺盘在25±5℃温度,80%±5%湿度,培养72-80h。当荞麦芽长到2-3cm后,每隔2-3小时喷洒一次水,待荞麦芽长到10-15cm,芽的子叶呈微黄,胚轴呈亮白色,终止发芽过程。Germination: Take out the soaked wheat and spread it on a tray at a temperature of 25±5°C and a humidity of 80%±5%, and cultivate it for 72-80h. When the buckwheat sprouts grow to 2-3cm, spray water every 2-3 hours. When the buckwheat sprouts grow to 10-15cm, the cotyledons of the sprouts are slightly yellow and the hypocotyls are bright white, and the germination process is terminated.
破壁:荞麦芽经喷淋水脱壳后,在高速剪切处理机中进行胚芽细胞破壁和微细化处理,其离心转速为2000-10000rpm,将其粉碎为30-50μm细度的浆体。Wall breaking: After the buckwheat germs are shelled by spraying water, the germ cells are broken and micronized in a high-speed shearing processor. .
酶解:加入中性蛋白酶(20-300U/g),于30-50℃(43℃最适)酶解1-5h。Enzymolysis: Add neutral protease (20-300U/g), and enzymolysis at 30-50°C (43°C is optimal) for 1-5h.
配料:加入蜂蜜(1-5%)、低聚糖浆(1-3%)、黄原胶(0.005-0.02%)、羧甲基纤维素钠(0.01-0.1%)、海藻酸钠(0.01-0.2%)、胡萝卜素(微量)于酶解后的荞麦芽浆中。Ingredients: add honey (1-5%), oligosaccharide syrup (1-3%), xanthan gum (0.005-0.02%), sodium carboxymethylcellulose (0.01-0.1%), sodium alginate (0.01- 0.2%), carotene (trace amount) in the buckwheat mash after enzymolysis.
均质:将上述料液加热至50-70℃(60℃最适)再经高压均质机15-40Mpa(25Mpa最适)均质化。Homogenization: Heat the above feed liquid to 50-70°C (60°C is the optimum) and then homogenize it through a high-pressure homogenizer at 15-40Mpa (25Mpa is the optimum).
灌装:将均质后的物料经130℃,1-3sUHT灭菌后在无菌条件下灌装入容器即为成品。或在一般条件下灌装入容器再经110-120℃灭菌(15-45min)后即为成品。Filling: After the homogenized material is sterilized at 130°C and 1-3s UHT, it is filled into the container under aseptic conditions to obtain the finished product. Or fill it into a container under normal conditions and then sterilize it at 110-120°C (15-45min) to be the finished product.
以上所述内容仅为本发明构思下的基本说明,而依据本发明的技术方案所作的任何等效变换,均应属于本发明的保护范围。The above content is only a basic description of the concept of the present invention, and any equivalent transformation made according to the technical solution of the present invention shall fall within the scope of protection of the present invention.
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| CN101390528B (en) * | 2008-11-06 | 2010-12-15 | 上海应用技术学院 | Preparation method of buckwheat bread premixing powder |
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