CN1650924A - Four component tumeric tablet and its preparation method - Google Patents
Four component tumeric tablet and its preparation method Download PDFInfo
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Abstract
A Chinese medicine in the form of tablet or capsule for treating urethral infection is prepared from turmeric, amur barberry bark, phyllanthus emblica and tribulus fruit through decocting and concentrating.
Description
Technical field
The present invention relates to a kind of medicine for the treatment of urinary tract infection.Be the Chinese patent medicine of feedstock production specifically, the invention still further relates to the preparation method of this medicine with the Chinese herbal medicine.
Background technology
Urinary tract infection is a modal class disease in all bacterial infections, and according to domestic investigation statistics, its sickness rate accounts for total population rate 0.9%, and women's sickness rate can be up to 2.05%, and is approaching with the report of the U.S..The primary disease men and women, old and young all can be taken place, and are common with the women especially.Have 30% women that the experience of urinary tract infection was arranged approximately, and 6% the once symptomatic urinary tract infection of the annual meeting appearance of women is arranged approximately, the ratio of this disease men and women sickness rate is 1: 9, and the ratio of married and unmarried morbidity is 12.8: 1.
Urinary tract infection mainly is the Urinary system inflammation that is caused by bacterial infection, and any pathogenic bacterium are invaded urinary tract all can cause urinary tract infection, and therefore, this disease is very easily common.
People's Republic of China's Tibetan medicine ministry standard discloses a kind of SIWEI JIANGHUANG TANGSAN, and it is made up of four Chinese medicine things such as Rhizoma Curcumae Longae, Radix Berberidis Amurensis, Fructus Phyllanthi, Fructus Tribulis, has heat clearing away, diuretic effect; Be used for urinary tract infections such as urethritis, frequent micturition, urgent micturition.This medicaments preparation method is that Rhizoma Curcumae Longae, Radix Berberidis Amurensis, Fructus Phyllanthi, Fructus Tribuli four Chinese medicine thing are ground into coarse powder, sieves mixing.When taking above powder decocted again and take, the method is inconvenience very, and other extraneous many effects limit are arranged, and the some drugs in this component is pulverized the back digestive tract, gastrointestinal tract are had stimulation.
Summary of the invention
The object of the present invention is to provide a kind of medicine four turmeric sheet of taking the treatment urinary tract infection easy, that dose is little.
Another object of the present invention is to provide a kind of preparation method of producing the availability height, improving this medicine of medicament contg.
Solution of the present invention is to be that dosage form and technological innovation are carried out in the basis with the SIWEI JIANGHUANG TANGSAN, to reduce dose, medicament curative effect enhancement.
Medicine of the present invention is tablet or the capsule of being made by following component: (consumption is a weight portion)
Rhizoma Curcumae Longae 10-15, Radix Berberidis Amurensis 8-12, Fructus Phyllanthi 15-25, Fructus Tribuli 15-25
The optimum weight of medicine of the present invention (part) proportioning is:
Rhizoma Curcumae Longae 12, Radix Berberidis Amurensis 10, Fructus Phyllanthi 20, Fructus Tribuli 20
Above-mentioned each component is made medicine production method of the present invention is:
1, Rhizoma Curcumae Longae, Radix Berberidis Amurensis are ground into coarse powder, according to the percolation under fluid extract and the extractum item, make solvent with 70%～95% ethanol, flood 20～30 hours, collect percolate;
2, percolate decompression recycling ethanol and be concentrated into the clear paste of relative density 1.20～1.40 (60～80 ℃);
3, clear paste and starch mixing, drying under reduced pressure is ground into fine powder;
4, Fructus Phyllanthi, Fructus Tribuli decoct with water three times, each 0.5～1 hour, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.10～1.15 (60～80 ℃), puts cold rnning ethanol and transfers to that to contain the alcohol amount be 60～80%, standing over night, decompression filtrate recycling ethanol and to be concentrated into relative density be 1.20～1.35 (60～80 ℃), drying is ground into fine powder;
5, mix above two kinds of fine powders, with proper auxiliary materials mixing, tabletting.
Clinical practice result of the present invention shows that following advantage is arranged:
1, handles medicament contg is raise through serial of methods such as decocting, concentrate;
2, drug administration method of the present invention is simple, and dose is few;
3, produce the availability height.
For showing that medicine of the present invention has significant curative effect to the treatment urethritis, medicine of the present invention proves to have good result through a series of zoopery.
One, diuresis experiment
1 experiment material
1.1 medicine and reagent
Medicine four turmeric sheet extract of the present invention: for the dark-brown extract powder, special odor is arranged, the 1.2g extract powder is equivalent to the 5g crude drug.Lot number: 020401, provide by Chengdu University of Traditional Chinese Medicine's national medicine institute.Be mixed with suspension with 0.5% carboxymethylcellulose sodium solution (CMC) before the experiment.The administration concentration of rat is 30%, and irritating stomach (ig) volume is the 1ml/100g body weight, and the administration concentration of mice is 15%, and irritating stomach (ig) volume is the 0.2ml/10g body weight.It is medicine group of the present invention, SIWEI JIANGHUANG SAN group, matched group, four groups of blank group that drug study is established four turmeric sheet group.
SIWEI JIANGHUANG TANGSAN (former dosage form): Chinese Lhasa Tibet Tibetan Medicine Plant produces, lot number: 011118.Adult's (60kg) consumption every day is the 9g crude drug in whole.(medicinal liquid of g crude drug in whole/100ml) is standby to be mixed with variable concentrations with 0.5% carboxymethylcellulose sodium solution (CMC) during experiment.The administration concentration of rat is 30%, and irritating stomach (ig) volume is the 1ml/100g body weight, and the administration concentration of mice is 15%, and irritating stomach (ig) volume is the 0.2ml/10g body weight.
Sodium carboxymethyl cellulose: Shanghai San Pu chemical industry company limited is produced, 800-1200 order, lot number: 20010911.Being mixed with concentration with distilled water is 0.5% solution.
Hydrochlorothiazide tablet: Lik-Sang pharmaceutical factory in Tianjin produces, lot number: 0101002, and authentication code: the accurate word (1981) of medicine is defended No. 001181 in Tianjin.Be mixed with 5% concentration (rat ig volume is 1ml/100g, and mice ig volume is 0.2ml/10g) with distilled water.
1.2 animal
The rat one-level, SD, complete male, body weight 200 ± 20g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 8th, the real moving pipe in river.
The mice one-level, the Kunming kind, male and female half and half, body weight 20 ± 2g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 7th, the real moving pipe in river.
1.3 instrument
Electronic balance Longteng Electronic Weighing Instrument Co., Ltd., Shenyang, model: LD600-1, precision: 0.1g
Electronic balance Sartorious, BP61, precision: 0.1mg
1.4 statistical method
Use SPSS 10.0 statistical packages to handle experimental data.Note amount data normal distribution data relatively adopt t check in groups for two groups, and many groups are relatively adopted variance analysis (LSD).The numeration data adopts Ridit to analyze.Down together.
1.5 method and result
1.5.1 influence to water load rat urine amount
Choose 40 of healthy male rats, be divided into 4 groups at random by body weight, first group of ig0.5%CMC is as the blank group; The CMC suspension of second and third group difference ig four turmeric sheet extract powder and SIWEI JIANGHUANG SAN is as the administration group; The 4th group of ig hydrochlorothiazide 1ml/100g, promptly 0.5g/kg is as positive controls.Be administered once every day, and for three days on end, ig distilled water 4ml/100g behind the last administration 1h as the water load, and gently presses the rat lower abdomen to drain surplus urine.Put into metabolic cage with being about to rat, every 2h writes down once urine amount, continuously 6h.The results are shown in Table 1.
Table 1 four turmeric sheet is to the influence of water load rat urine amount (X ± SD)
Grouping dosage number of animals urine amount (ml)
((only) 2h 4h 6h total amount of g crude drug/kg)
Blank-----10 1.65 ± 0.27 0.78 ± 0.56 0.14 ± 0.18 2.55 ± 0.55
Four turmeric sheet 3.0 10 2.10 ± 0.57
*1.12 ± 0.79 0.60 ± 0.34
*3.82 ± 0.98
*
SIWEI JIANGHUANG SAN 3.0 10 1.70 ± 0.55 0.51 ± 0.23 0.96 ± 0.25
*3.17 ± 0.61
Hydrochlorothiazide 0.5 10 3.04 ± 0.34
*2.32 ± 0.78
*0.97 ± 0.85
*6.33 ± 0.78
*
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 1 is the result show: the four turmeric sheet can obviously increase water load rat urine amount (p＜0.05) than SIWEI JIANGHUANG SAN.
1.5.2 influence to water load mouse retention amount
Choose 40 of male mices, be divided into 4 groups at random by body weight, first group of ig0.5%CMC (0.2ml/10g) is as the blank group; The CMC suspension of second and third group difference ig four turmeric sheet extract powder and SIWEI JIANGHUANG SAN is as the administration group; The 4th group of ig hydrochlorothiazide 1g/kg is as positive controls.Be administered once every day, and for three days on end, ig distilled water 1ml/ only as the water load, and gently presses the mice lower abdomen to drain surplus urine behind the last administration 1h.Put into the beaker that is lined with filter paper with being about to mice, per hour change paper once, weighing filter paper, and the weightening finish of record filter paper is as the value of urine amount, 4h continuously.The results are shown in Table 2.
Table 2 four turmeric sheet is to the influence of water load mouse retention amount (X ± SD)
Grouping dosage number of animals urine amount (g)
(crude drug g/kg) (only) 1h 2h 3h 4h total amount
Blank-----10 0.81 ± 0.23 0.11 ± 0.11 0.07 ± 0.06 0.06 ± 0.05 1.04 ± 0.22
Four turmeric sheet 3.0 10 1.11 ± 0.47
*0.36 ± 0.21
*0.13 ± 0.17
*0.23 ± 0.20
*1.76 ± 0.42
*
SIWEI JIANGHUANG SAN 3.0 10 0.86 ± 0.38 0.26 ± 0.26 0.07 ± 0.07 0.07 ± 0.08 0.20 ± 0.15
*
Hydrochlorothiazide 1.0 10 0.94 ± 0.27
*0.37 ± 0.27
*0.08 ± 0.08 0.17 ± 0.10
*1.61 ± 0.35
*
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 2 is the result show: the four turmeric sheet aspect the urine amount that increases water load mice effect than SIWEI JIANGHUANG SAN more obviously (p＜0.01).
Two, antiinflammatory experiment
1 experiment material
1.1 medicine and reagent
Four turmeric sheet extract powder: test with diuresis.
SIWEI JIANGHUANG TANGSAN (former dosage form): test with diuresis.
Carrageenin (Carrageenan), Sigma Co.Being mixed with concentration with normal saline is 1% solution.
The blue Shanghai chemical reagent purchasing and supply station import packing of ivens, 010401.
Dexamethasone sodium phosphate injection Zhejiang Province fairy house pharmaceutical Co. Ltd produces, lot number: 010828, and authentication code: the accurate word (1996) of medicine is defended No. 118102 in Zhejiang.Be mixed with 0.05% solution with normal saline.
1.2 animal
The rat one-level, SD, complete male, body weight 200 ± 20g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 8th, the real moving pipe in river.
The mice one-level, the Kunming kind, complete male, body weight 20 ± 2g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 7th, the real moving pipe in river.
1.3 instrument
Spectrophotometer Prism Optical Technology Co, Spectrumlab, 22pc
1.4 method and result
1.4.1 influence to the mouse peritoneal capillary permeability
Choose 40 of healthy mices, be divided into 4 groups at random by body weight, first group of ig gives 0.5%CMC solution (0.2ml/10g) as the blank group; Second and third group respectively ig gives CMC suspension with four turmeric sheet extract powder and SIWEI JIANGHUANG SAN as the administration group; The 4th group of intramuscular injection (im) dexamethasone sodium phosphate 0.1ml/10g is as positive controls.Be administered once every day, for three days on end, and tail vein injection 1% Yi Wensilan normal saline solution 0.1ml/10g, lumbar injection (ip) 0.6% acetic acid 0.1ml/10g immediately behind the last administration 30min.Behind the 20min mice is taken off neck and put to death, cut off the abdominal cavity,, merge flushing liquor, get supernatant behind centrifugal (3000r/min) 10min, measure absorbance in the 610nm place with 6ml normal saline flushing abdominal cavity.The results are shown in Table 3.
Table 3 four turmeric sheet is to the influence of mouse peritoneal capillary permeability (X ± SD)
Grouping dosage number of animals absorbance
(g/kg) (only) (OD value)
Blank----10 1.09 ± 0.29
Four turmeric sheet 3.0 10 0.82 ± 0.14*
SIWEI JIANGHUANG SAN 3.0 10 1.03 ± 0.39
Dexamethasone 5mg 10 0.74 ± 0.30*
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 3 is the result show: be better than four turmeric powder (p＜0.05) aspect the mouse peritoneal capillary permeability of four turmeric sheet due to inhibition acetic acid.
1.4.2 Ovum Gallus domesticus album is caused the influence of rat paw edema
Get 40 of healthy rats, be divided into 4 groups at random by body weight.First group of ig0.5%CMC is as the blank group; The CMC suspension of second and third group difference ig four turmeric sheet extract powder and SIWEI JIANGHUANG SAN is as the administration group; The 4th group of lumbar injection (ip) 5mg/kg dexamethasone is as positive controls.Administration every day 1 time, for three days on end.Mark at the right back ankle joint of each Mus before the experiment, measure each Mus foot volume twice by sufficient volumetric method, averaging causes scorching preceding normal foot volume as each Mus.30min after the last administration, every subcutaneous inserting needle of the right back sufficient sole of the foot of rat is to subcutaneous near the ankle joint, inject 10% fresh Ovum Gallus domesticus album solution 0.1ml and cause inflammation, ig normal saline (4ml/100g) row water is loaded simultaneously, respectively at cause scorching back 60,120,240,360min measures each Mus right hind foot volume with method, observe causing scorching before and after the volumetrical changing value of rat foot.Calculate the scorching front foot volume of the scorching metapedes volume of swelling degree=cause-cause.The results are shown in Table 4.
Table 4 four turmeric sheet causes the influence (X ± SD) of rat paw edema to Ovum Gallus domesticus album
Grouping dosage number of animals causes scorching front foot volume swelling degree (ml)
(g/kg) (only) (ml) 30min 60min 120min 240min
Blank-10 1.39 ± 0.13 0.72 ± 0.19 0.90 ± 0.16 0.82 ± 0.14 0.67 ± 0.18
Four turmeric sheet 3.0 10 1.48 ± 0.09 0.37 ± 0.21**, 0.76 ± 0.12*, 0.54 ± 0.16**, 0.35 ± 0.11**
SIWEI JIANGHUANG SAN 3.0 10 1.47 ± 0.08 0.56 ± 0.20 0.88 ± 0.18 0.79 ± 0.24 0.49 ± 0.25*
Dexamethasone 5mg 10 1.37 ± 0.14 0.35 ± 0.24**, 0.29 ± 0.20**, 0.32 ± 0.19**, 0.12 ± 0.17**
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 4 is the result show: four turmeric sheet and SIWEI JIANGHUANG SAN have obvious inhibitory action to rat paw edema due to the Ovum Gallus domesticus album, but the four turmeric sheet is than SIWEI JIANGHUANG SAN effective (p＜0.05～0.01).
1.4.3 on Carrageenan causes the influence of rat paw edema
Get 40 of male rats, be divided into 4 groups at random by body weight.First group of ig gives 0.5%CMC as the blank group; Second and third organizes the CMC suspension that ig respectively gives four turmeric sheet extract powder and SIWEI JIANGHUANG SAN, as the administration group; The 4th group of ip dexamethasone 5mg/kg is as positive controls.Be administered once every day, for three days on end.Mark at the right back ankle joint of each Mus before the experiment, measure each Mus foot volume twice by sufficient volumetry, averaging causes scorching preceding normal foot volume as each Mus.30min after the last administration, every subcutaneous inserting needle of the right back sufficient sole of the foot portion of rat is to subcutaneous near the ankle joint, inject 1% carrageenin 0.1ml and cause inflammation, ig normal saline (4ml/100g) row water is loaded simultaneously, respectively at cause scorching back 60,120,240,360min measures each Mus right hind foot volume with method, observe causing scorching before and after the volumetrical changing value of rat foot.The scorching front foot volume of the scorching metapedes volume of swelling degree=cause-cause.The results are shown in Table 5.
Table 5 four turmeric sheet on Carrageenan causes the influence (X ± SD) of rat paw edema
Grouping dosage number of animals causes scorching front foot volume swelling degree (ml)
(g/kg) (only) (ml) 60min 120min 240min 360min
Blank-10 1.42 ± 0.08 0.17 ± 0.06 0.22 ± 0.08 0.26 ± 0.07 0.20 ± 0.10
Four turmeric sheet 3.0 10 1.45 ± 0.05 0.05 ± 0.06*, 0.05 ± 0.05**, 0.04 ± 0.04**, 0.05 ± 0.09**
SIWEI JIANGHUANG SAN 3.0 10 1.47 ± 0.08 0.05 ± 0.05* 0.11 ± 0.08 0.18 ± 0.07 0.16 ± 0.06
Dexamethasone 5mg 10 1.41 ± 0.11 0.06 ± 0.08*, 0.04 ± 0.05**, 0.04 ± 0.06**, 0.03 ± 0.05**
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 5 is the result show: the four turmeric sheet is suppressing obviously to be better than SIWEI JIANGHUANG TANGSAN (p＜0.05～0.01) aspect the foot swelling that carrageenin causes.
1.4.4 influence to the mice granuloma induced by implantation of cotton pellets
Get 40 of healthy male mices, after the sterilization of left side skin of groin, eye scissors is cut an osculum, and it is subcutaneous with the ophthalmology tweezer autoclaving cotton balls (10 ± 0.5mg, gentamycin liquid soaks into) to be implanted the right side of mice axillary fossa.By body weight animal is divided into 4 groups at random then, first group of ig gives 0.5%CMC as the blank group; Second and third organizes the 0.5%CMC suspension that ig respectively gives four turmeric sheet extract powder and SIWEI JIANGHUANG SAN, as the administration group; The 4th group of ip dexamethasone 5mg/kg is as positive controls.Successive administration 7 days behind the last administration 24h, takes out cotton balls together with connective tissue on every side, reject fatty tissue, dry in 70 ℃ of baking ovens, weigh, with claim the weight former weight that deducts cotton balls promptly get granuloma weight, add up with granulation ponderal index (granulation weight/body weight).The results are shown in Table 6.
Table 6 four turmeric sheet is to the influence of mice granuloma induced by implantation of cotton pellets (X ± SD)
Grouping dosage number of animals granulation weight granulation ponderal index
(crude drug g/kg) (only) be (granulation weight mg/ body weight g) (mg)
Blank-10 5.53 ± 1.57 0.28 ± 0.09
Four turmeric sheet 3.0 10 3.54 ± 0.63* 0.18 ± 0.03*
SIWEI JIANGHUANG SAN 3.0 10 5.16 ± 2.17 0.24 ± 0.12
Dexamethasone 5mg 10 3.39 ± 0.63* 0.17 ± 0.04*
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 6 is the result show: the four turmeric sheet is suppressing obviously to be better than four turmeric powder (p＜0.05) aspect the granulation growth that cotton balls causes.
Three, analgesic experiment
1 experiment material
1.1 medicine and reagent
Four turmeric sheet extract powder: test with diuresis.
SIWEI JIANGHUANG TANGSAN (former dosage form): test with diuresis.
Morphine hydrochloride: Shenyang No. 1 Pharmaceutical Factory, lot number: 98050, authentication code: the Liao Dynasty defends the accurate word (1982) of medicine No. 001213.Be mixed with 5mg/kg (0.1ml/10g) with normal saline.
1.2 animal
The mice one-level, the Kunming kind, male and female half and half, body weight 20 ± 2g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 7th, the real moving pipe in river.
1.3 instrument
Electronic balance Longteng Electronic Weighing Instrument Co., Ltd., Shenyang, model: LD600-1, precision: 0.1g
Electronic balance Sartorious, BP61, precision: 0.1mg
Hot plate dolorimeter GJ-8402 type, the white stone electronics medicine in Ninghai County, Zhejiang instrument plant
1.4 method and result
1.4.1 Dichlorodiphenyl Acetate causes the influence of mouse writhing reaction
Get 40 of healthy mices, male and female half and half, be divided into 4 groups at random by body weight, first group of ig gives 0.5%CMC as the blank group, the CMC suspension that second and third group ig gives four turmeric sheet extract powder and SIWEI JIANGHUANG SAN is as the administration group, and the 4th group of sc morphine hydrochloride 0.05g/kg is as positive controls.Except that positive controls before experiment the administration 1 time, all the other respectively organize continuous ig administration 3 days, 30min after the last administration, ip in mice 0.6% acetum 0.2ml/ only, clock immediately, observe and write down the number of times (with the mouse web portion depression, two hind legs are stretched to the writhing response index) of writhing response in the mice 15min, the results are shown in Table 7.
Table 7 four turmeric sheet Dichlorodiphenyl Acetate causes the influence (X ± SD) of mouse writhing reaction
Turn round the body number in the grouping dosage number of animals 15min
(g/kg) (only) (inferior)
Blank----10 37.80 ± 11.99
Four turmeric sheet 3.0 10 19.50 ± 7.18
*
SIWEI JIANGHUANG SAN 3.0 10 34.80 ± 17.38
Morphine 0.05 10 2.60 ± 5.80
*
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 7 is the result show: the four turmeric sheet stimulates and obviously is better than four turmeric powder (p＜0.05) aspect the pain that causes suppressing acetic acid.
1.4.2 hot plate is stimulated the influence of induced mice pain reaction
Get the Healthy female mice, utilize twice threshold of pain meansigma methods of hot plate dolorimeter (hot plate temperature is 55 ± 0.5 ℃) screening (pain threshold is for placing the time of licking metapedes on the hot plate to it with mice) to be lower than 30s and to be qualified animal greater than 5s person.Get 40 of qualified animals, be divided into 4 groups at random by body weight, first group of ig0.5%CMC (0.2ml/10g) be as the blank group, second and third group respectively CMC suspension of ig four turmeric sheet extract powder and SIWEI JIANGHUANG SAN as the administration group, the 4th group of ip morphine hydrochloride 0.05g/kg.Before the administration and after the administration 15,30,60,90min measures each Mus pain threshold respectively, and calculates the analgesia percentage rate, pain threshold % before analgesia percentage rate=(after the administration before pain threshold-administration pain threshold)/administration the results are shown in Table 8.
Table 8 four turmeric sheet stimulates the influence (X ± SD) of induced mice pain reaction to hot plate
Pain threshold (analgesia percentage rate %) after the pain threshold administration before the administration of grouping dosage number of animals
(g/kg) (only) (sec) 15min 30min 60min 90min
Blank-10 17.59 ± 43.5 18.65 ± 8.70 (5) 17.54 ± 8.11 (0) 19.33 ± 9.07 (9) 20.65 ± 11.03 (17)
Four turmeric sheet 3.0 10 17.55 ± 2.54 23.17 ± 3.26 (32) 27.55 ± 43.7 (57)
*25.97 ± 5.21 (48) 27.73 ± 3.21 (58)
*
SIWEI JIANGHUANG SAN 3.0 10 18.21 ± 0.78 20.88 ± 5.12 (16) 21.42 ± 5.23 (19) 21.60 ± 1.65 (20) 21.24 ± 5.21 (18)
Morphine 0.05 10 16.60 ± 3.54 29.23 ± 5.48 (76)
*28.40 ± 2.56 (71)
*29.57 ± 5.21 (78)
*31.23 ± 2.34 (88)
*
Compare with the blank group:
*P＜0.01,
*P＜0.05
Table 8 is the result show: the four turmeric sheet is suppressing obviously to be better than four turmeric powder (p＜0.05) aspect the pain that thermostimulation causes.Four, analgesic experiment
1 experiment material
1.1 medicine and reagent
Four turmeric sheet extract powder: test with diuresis.
SIWEI JIANGHUANG TANGSAN (former dosage form): test with diuresis.
Carrageenin: test with diuresis.
Aspirin Shijiazhuang Shineway Pharmaceutical Co., Ltd produces, lot number: 01080611, and authentication code: the accurate word (1995) of medicine is defended No. 010632 in the Ji.Be mixed with 1% (g/100ml), rat ig1ml/100g, i.e. 0.1g/kg with normal saline.
1.2 animal
The rat one-level, SD, complete male, body weight 200 ± 20g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 8th, the real moving pipe in river.
1.3 instrument
Electronic balance Longteng Electronic Weighing Instrument Co., Ltd., Shenyang, model: LD600-1, precision: 0.1g
Anus temperature table Sharp corporation Japan, MT-20
1.4 method and result
1.4.1 on Carrageenan causes the influence that rat temperature raises
Get 40 of healthy male rats, be divided into 4 groups at random by body weight.First group of ig0.5%CMC is as the blank group; The CMC suspension of second and third group difference ig four turmeric sheet and SIWEI JIANGHUANG SAN is as the administration group; The 4th group of ig aspirin is as positive controls.Be administered once every day, and successive administration 3 days is measured twice rat anus temperature before the modeling, average as normal body temperature before each Mus administration.30min modeling after the last administration, from every subcutaneous inserting needle of the right back sufficient sole of the foot portion of rat to subcutaneous near the ankle joint, inject 1% carrageenin 0.1ml, respectively at after the modeling 60,120,240,360min measures each Mus anus temperature, body temperature before body temperature-modeling after fervescence value=modeling (℃).The results are shown in Table 9.
Table 9 four turmeric sheet on Carrageenan causes the influence that rat temperature raises (X ± SD)
Group dosage number of animals normal body temperature fervescence value (℃)
(crude drug g/kg) (only) (℃) 1h 2h 4h 6h
Blank-10 36.3 ± 0.53 0.71 ± 0.33 0.98 ± 0.32 0.95 ± 0.38 0.71 ± 0.35
Four turmeric sheet 3.0 10 36.3 ± 0.61 0.55 ± 0.46 0.44 ± 0.32**, 0.51 ± 0.30** 0.60 ± 0.23
SIWEI JIANGHUANG SAN 3.0 10 36.8 ± 0.68 0.45 ± 0.31 0.55 ± 0.38 0.69 ± 0.52 0.65 ± 0.40
Aspirin 0.1 10 36.4 ± 0.67 0.23 ± 0.27**, 0.38 ± 0.33**, 0.55 ± 0.23*, 0.30 ± 0.23**
Compare with the blank group: * * p＜0.01, * p＜0.05
Table 9 is the result show: the four turmeric sheet is suppressing obviously to be better than SIWEI JIANGHUANG SAN aspect the rat temperature rising.
Five, antibacterial experiment---to infecting the influence of colon bacillus time-to-live in the mice body
1 experiment material
1.1 medicine and reagent
Four turmeric sheet extract powder: test with diuresis.
SIWEI JIANGHUANG TANGSAN (former dosage form): test with diuresis.
Ofloxacin SHANXI DEYUANTANG PHARMACEUTICAL CO.,LTD, authentication code: the accurate word (1996) of medicine is defended No. 174001 in the Shanxi, lot number: 020130.Be mixed with 0.5% (g/100ml), mice ig0.2ml/10g with normal saline.Be 0.1g/kg.
1.2 experimental strain
Colon bacillus 0111B4, bacterium number: 49155, provide by Chengdu University of Traditional Chinese Medicine's microorganism and immunology teaching and research room.
1.3 animal
The mice one-level, the Kunming kind, male and female half and half, body weight 20 ± 2g is provided by Chengdu University of Traditional Chinese Medicine's Experimental Animal Center.The animal quality certification number: No. the 7th, the real moving pipe in river.
1.4 method and result
1.4.1 prerun experiment
Choose 18 of healthy mices, be divided into 3 groups at random by body weight, 6 every group, (concentration is respectively 1 * 107CFU/ml to the ip variable concentrations, 1 * 108CFU/ml, 1 * 109CFU/ml) colon bacillus bacterium liquid 0.4ml/ only seeks the bacterial concentration that causes 80～90% dead mouses, and result of the test shows, ip concentration is bacterium liquid 0.4ml/ of 1 * 109CFU/ml, cause 5 dead mouses, mortality rate is near 90%, so select this bacterial concentration formally to test.
1.4.2 formal experiment---to infecting the influence of colon bacillus time-to-live in the mice body
Choose 40 of healthy mices, be divided into 4 groups at random by body weight, first group of ig0.5%CMC is as the blank group; The CMC suspension of second and third group difference ig four turmeric sheet extract powder and the CMC suspension of SIWEI JIANGHUANG SAN are as the administration group; The 4th group of ig ofloxacin 0.1g/kg is as positive controls.Once a day, successive administration 3 days, the 3rd day ip causes the colon bacillus bacterium liquid (1 * 109CFU/ml, 0.4ml/ are only) of 90% dead mouse.The survival number of 3 days mices the results are shown in Table 10 after the record infection colon bacillus.
Table 10 four turmeric sheet is to infecting the influence of colon bacillus in the mice body
Grouping dosage number of animals survival number death toll mortality rate
(crude drug g/kg) (only) (only) (only) (%)
Blank----10 19 90
Four turmeric sheet 3.0 10 64 40
*
SIWEI JIANGHUANG SAN 3.0 10 28 80
Ofloxacin 100mg 10 10 00
*
Table 10 is the result show: the four turmeric sheet is better than SIWEI JIANGHUANG SAN aspect the infection in protection colon bacillus body.
In sum, by 10 pharmacological evaluation in above 5 aspects, the result shows: the curative effect of the medicament four turmeric sheet that employing the method for the invention makes is better than existing SIWEI JIANGHUANG SAN at diuresis, antiinflammatory, analgesia, antibiosis.
The specific embodiment
What below introduce is embodiments of the invention, but scope of the present invention is not limited to following example.
Embodiment 1:
Medicine of the present invention is tablet or the capsule of being made by following component: (consumption is a weight portion)
Rhizoma Curcumae Longae 10, Radix Berberidis Amurensis 8, Fructus Phyllanthi 15, Fructus Tribuli 15
More than four the flavor, Rhizoma Curcumae Longae, Radix Berberidis Amurensis are ground into coarse powder, according to the percolation under fluid extract and the extractum item, make solvent with ethanol 70%, flooded 20 hours, collect percolate, the percolate decompression recycling ethanol also is concentrated into the clear paste of relative density 1.25 (60 ℃), clear paste and starch mixing, drying under reduced pressure is ground into fine powder; Fructus Phyllanthi, Fructus Tribuli decoct with water three times, each 0.5 hour collecting decoction, filter, it is 1.10 (60 ℃) clear paste that filtrate is concentrated into relative density, puts cold rnning ethanol and transfers to that to contain the alcohol amount be 60%, standing over night, decompression filtrate recycling ethanol and to be concentrated into relative density be 1.25 (60 ℃), drying is ground into fine powder, mix above two kinds of fine powders, with the appropriate amount of starch mixing; Add microcrystalline Cellulose, Pulvis Talci, mix homogeneously, tabletting get the four turmeric sheet, and filled capsules just gets the four turmeric capsule.
Embodiment 2:
Medicine of the present invention is tablet or the capsule of being made by following component: (consumption is a weight portion)
Rhizoma Curcumae Longae 12, Radix Berberidis Amurensis 10, Fructus Phyllanthi 20, Fructus Tribuli 20
More than four the flavor, Rhizoma Curcumae Longae, Radix Berberidis Amurensis are ground into coarse powder, according to the percolation under fluid extract and the extractum item, make solvent with ethanol 80%, flooded 24 hours, collect percolate, the percolate decompression recycling ethanol also is concentrated into the clear paste of relative density 1.30 (70 ℃), clear paste and starch mixing, drying under reduced pressure is ground into fine powder; Fructus Phyllanthi, Fructus Tribuli decoct with water three times, each 45 minutes collecting decoctions, filter, it is 1.12 (60 ℃) clear paste that filtrate is concentrated into relative density, puts cold rnning ethanol and transfers to that to contain the alcohol amount be 70%, standing over night, decompression filtrate recycling ethanol and to be concentrated into relative density be 1.30 (70 ℃), drying is ground into fine powder, mix above two kinds of fine powders, with the appropriate amount of starch mixing; Add microcrystalline Cellulose, Pulvis Talci, mix homogeneously, tabletting get the four turmeric sheet.
Embodiment 3:
Medicine of the present invention is tablet or the capsule of being made by following component: (consumption is a weight portion)
Rhizoma Curcumae Longae 15, Radix Berberidis Amurensis 12, Fructus Phyllanthi 25, Fructus Tribuli 25
More than four the flavor, Rhizoma Curcumae Longae, Radix Berberidis Amurensis are ground into coarse powder, according to the percolation under fluid extract and the extractum item, make solvent with ethanol 90%, flooded 28 hours, collect percolate, the percolate decompression recycling ethanol also is concentrated into the clear paste of relative density 1.28 (80 ℃), clear paste and starch mixing, drying under reduced pressure is ground into fine powder; Fructus Phyllanthi, Fructus Tribuli decoct with water three times, each 1 hour collecting decoction, filter, it is 1.14 (80 ℃) clear paste that filtrate is concentrated into relative density, puts cold rnning ethanol and transfers to that to contain the alcohol amount be 80%, standing over night, decompression filtrate recycling ethanol and to be concentrated into relative density be 1.35 (70 ℃), drying is ground into fine powder, mix above two kinds of fine powders, with appropriate amount of starch or other auxiliary materials and mixing; Add microcrystalline Cellulose, Pulvis Talci, mix homogeneously, tabletting get the four turmeric sheet.
Embodiment 4:
Medicine of the present invention is tablet or the capsule of being made by following component: (consumption is a weight portion)
Rhizoma Curcumae Longae 15, Radix Berberidis Amurensis 10, Fructus Phyllanthi 20, Fructus Tribuli 20
More than four the flavor, Rhizoma Curcumae Longae, Radix Berberidis Amurensis are ground into coarse powder, according to the percolation under fluid extract and the extractum item, % makes solvent with alcohol 95, floods 30 hours, collects percolate, the percolate decompression recycling ethanol also is concentrated into the clear paste of relative density 1.40 (60 ℃), clear paste and starch mixing, drying under reduced pressure is ground into fine powder; Fructus Phyllanthi, Fructus Tribuli decoct with water three times, each 1 hour collecting decoction, filter, it is 1.15 (80 ℃) clear paste that filtrate is concentrated into relative density, puts cold rnning ethanol and transfers to that to contain the alcohol amount be 80%, standing over night, decompression filtrate recycling ethanol and to be concentrated into relative density be 1.35 (80 ℃), drying is ground into fine powder, mix above two kinds of fine powders, with appropriate amount of starch or other auxiliary materials and mixing; Add microcrystalline Cellulose, Pulvis Talci, mix homogeneously, tabletting get the four turmeric sheet.
Claims (4)
1, a kind of four turmeric sheet is characterized in that: it is tablet or the capsule of being made by the following weight parts proportion raw material: Rhizoma Curcumae Longae 10-15, Radix Berberidis Amurensis 8-12, Fructus Phyllanthi 15-20, Fructus Tribuli 15-20.
2, four turmeric sheet as claimed in claim 1, wherein the weight portion proportioning of each raw material is: Rhizoma Curcumae Longae 12, Radix Berberidis Amurensis 10, Fructus Phyllanthi 20, Fructus Tribuli 20.
3, the preparation method of four turmeric sheet as claimed in claim 1 or 2, it is characterized in that: Rhizoma Curcumae Longae, Radix Berberidis Amurensis are ground into coarse powder, according to the percolation under fluid extract and the extractum item, make solvent with 70%～95% ethanol, flooded 20～30 hours, collect percolate, the percolate decompression recycling ethanol also is concentrated into the clear paste of relative density 1.20～1.40 (60～80 ℃), clear paste and starch mixing, drying under reduced pressure is ground into fine powder; Fructus Phyllanthi, Fructus Tribuli decoct with water three times, each 0.5～1 hour, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.10～1.15 (60～80 ℃), puts cold rnning ethanol and transfers to that to contain the alcohol amount be 60～80%, standing over night, decompression filtrate recycling ethanol and to be concentrated into relative density be 1.20～1.35 (60～80 ℃), drying is ground into fine powder; Mix above two kinds of fine powders, with the proper auxiliary materials mixing, tabletting gets finished product.
4, the preparation method of four turmeric sheet as claimed in claim 3 is characterized in that: described adjuvant or starch or microcrystalline Cellulose or Pulvis Talci.
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| CN1318082C (en) * | 2005-01-10 | 2007-05-30 | 西藏藏药股份有限公司<Del/> | Medicinal composition for treating chronic prostatitis and its preparation method and use |
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| CN108567955A (en) * | 2017-03-08 | 2018-09-25 | 成都中医药大学 | A kind of pharmaceutical composition and preparation method thereof of prevention diabetic nephropathy |
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| CN1318082C (en) * | 2005-01-10 | 2007-05-30 | 西藏藏药股份有限公司<Del/> | Medicinal composition for treating chronic prostatitis and its preparation method and use |
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