CN1648668A - Integrated type electrochemical biochemical sensing device and its sensing method - Google Patents
Integrated type electrochemical biochemical sensing device and its sensing method Download PDFInfo
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- CN1648668A CN1648668A CN 200410002433 CN200410002433A CN1648668A CN 1648668 A CN1648668 A CN 1648668A CN 200410002433 CN200410002433 CN 200410002433 CN 200410002433 A CN200410002433 A CN 200410002433A CN 1648668 A CN1648668 A CN 1648668A
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Abstract
The present invention provides integrated electrochemical and biochemical sensing device and method. The integrated electrochemical and biochemical sensing device includes at least one sampler, several electrode sets, one multipath electrochemical sensing unit, one operation logic and data acquisition unit, and one signal display to show the physiological parameter values and corresponding concentration of tested biological body liquid sample. The sensing device has blood sampling, several continuous tests, multipath sensing, work voltage regulation, etc. integrated, and may be used to obtain test result of several physiological parameters in once test. The present invention has raised use convenience and high use value.
Description
Technical field
The present invention relates to a kind of integrated galvanochemistry biochemical sensing device and its method for sensing, especially to utilize a kind of integrated module to carry out repeatedly biosome biochemical sensing with several samples.
Background technology
Biosensing apparatus and bioelectronics product are the instruments that utilizes the acting force of biomolecule characteristic to be used as checking.Biology sensor utilizes biomolecule (as protein, gene, ferment, antibody, nucleic acid etc.) as the identification assembly, the bioelectronics product is on the inorganic semiconductor material of tradition (as silicon), place particular organisms material (as materials such as protein molecules), with other material specific biochemical reaction takes place after, but the message real-time quantitative that is discharged, binding signal transmission, signal Processing and be performed on one are applied in multiple uses such as medical test, food inspection, agriculture monitoring, environmental protection, industry, pharmacy.
The development trend of global aging society makes home care type medical equipment industry become the main flow of global medical equipment development, for the also increase day by day of demand of house type/portable/personalized Physiology and biochemistry sensing apparatus.For example: the uric acid biochemical sensor of the glucose biochemical sensor of check diabetes physiological parameter and glycated protein biochemical sensor, check gout physiological parameter, the triglyceride of check angiocardiopathy correlation parameter and the biochemical sensor of cholesterol level, the blood oxygen concentration sensor of check cardio-pulmonary function and development of portable electrocardiogram machine or the like.Wherein everybody is paid attention to the most with development with the relevant check of diabetes again.Diabetes are a kind of chronic diseases, and sufferer quantity is constantly grown up, and at present the treatment of this disease are not still had good recipe, and people have spent a large amount of time and moneys in the diagnosis and treatment of this diabetes.In the amount of money that the health insurance body of the U.S. is paid every year, have surpass 1,000 hundred million dollars relevant with the diagnosis and treatment of diabetes, the unemployment cost relevant with diabetes is then more considerable.The World Health Organization (WHO) (The World Health Organization) sends alert news with the situation that U.S. disease Control Centre (U.S.Center forDisease Control) has all increased global patient of diabetes fast.
Chronic sufferer must just be understood the situation of health for a long time regularly by measuring the content of various physiological parameters in the blood, and this is to check method the most accurately at present.Therefore present various physiological parameter test mode on the market, all be to obtain one with acupuncture treatment to bleed liquid for check, though the development that non-intrusion type Wristwatch-type sensing apparatus is also arranged is arranged, and come out by the FDA authentication, but because this non-intrusion type Wristwatch-type sensing apparatus is to measure secreted physiological parameter in the skin sweat, be subjected to the influence of a large amount of perspirations of ambient weather and sufferer or the like, the physiological parameter numerical value that measures is accurate inadequately, therefore still need usually utilize traditional sensing apparatus to measure back comparison mutually.
In addition, many complication of chronic disease sufferer also can't merely be judged by single physiological parameter concentration in the detection blood, the physio-parameter detection system of multiparameter is considerable for the chronic disease sufferer, for example: the physiological detection of angiocardiopathy risk factor, and the detection of uric acid composition also is a present indispensable physiological parameter in the blood; Because the progress of society and the change of eating habit, the disease that uric acid is relevant is also perplexing many patients.But up to now, the house of bright rare multi-physiological-parameter is detected or development of individual checkout equipment or appearance.Most of houses type/portable/personalized physical parameter detector all must cooperate with blood sampling apparatus to be used in the lump, and different physiological parameters detects must be used different detection test pieces even change detection module, can learn different physiological parameters, also inconvenient in the use.
Being familiar with this physiology biochemical sensor product dealer all knows, the development of the biochemical sensor of same use of all kinds has been a suitable proven technique, present product on the market, great majority are developed at blood sugar test, comprising: the All-In-OneBD Latitude that Becton Dickinson company is developed
TMDiabetes Management System and New BD Logic
TMThe Precision QID blood sugar machine that BloodGlucose Monitor, MediSense company are developed, Elite blood sugar machine row product, the Hypoguard company that Bayer company is developed one of develop Accu-Chek Compact meter that the designed Ultra meter of row analysis of blood sugar product, Lifescan company and Roche company developed or the like, but also have at uric acid molecular conecentration and angiocardiopathy correlation factor in the detection blood, as: the CardioCheck PA that Polymer Technology System Inc. is developed
TMThe row product.Yet these checkout equipments mostly need collocation blood sampling apparatus and exclusive detection test paper in addition, even must change the module of different detection exclusive disjunctions during use, can do the check of single physiological parameter.
The All-In-One BD Latitude that Becton Dickinson company is developed
TMDiabetesManagement System, this design combines blood-taking device, detection test piece packaging together with sensing apparatus, but still must use respectively, and only this design makes that carrying of sufferer is comparatively convenient.Small part then in conjunction with blood-taking device, detect test paper and detection system, the Sof-Tact that is developed as Glucerna company
TMThe blood sugar machine, this blood sugar machine can be inserted blood taking needle and inspection test piece in the pick-up unit in advance in advance, and can plan the time-histories checked in advance, in addition, this system utilizes a small size vacuum pumping to gather blood sample, when machine is adjacent to skin, vacuum pump picks up skin, blood taking needle utilizes the effect of vacuum pump to make blood flow out rapidly in skin-piercing one small holes at this moment, and directly carries out the check of physiological parameter after the blood utilization check city sheet collection that will flow out; In addition, also have in conjunction with the design of pda system as sufferer medication and pathology monitoring, the variation that makes patient can note down administration time and grasp individual physiological parameter in detail, as: the OneTouch UltraSmart that Lifescan company is developed
TMBlood GlucoseMonitoring System, this system can be by input personal health condition, morbidity experience, medication experience, use medicine diet situation or the like at present, and becomes the diabetes-management system of peopleization one by one in conjunction with the blood sugar test result; The FreeStyleTracker system that TheraSense company is developed then ought time testing result, is past detected record, detected and note down or the like digitizing function the same day, develop the glucose detection instrument that a new generation in conjunction with PDA, and this system has passed through the approval of U.S. food drug abuse test office.
Previous technology all will be taken a blood sample and be separated with trace routine, therefore, must utilize blood taking needle to poke skin when the user uses and obtain blood sample, prepare a clean test piece simultaneously and obtain the analysis of can testing of enough blood samples.Can learn that by above program the user must prepare blood sampling apparatus, test piece and sensing instrument at any time, carry the inconvenience that seems relatively.And because single check can only be checked a kind of parameter, if need the different physiological parameters of check then must repeat above-mentioned inspection process again, and change and detect test piece, even the control module of replacing detection, the convenience deficiency of using, simultaneously, blood sampling apparatus and test piece abandon with aftertreatment also be other puzzlement.
Summary of the invention
In view of this, the purpose of this invention is to provide a kind of integrated galvanochemistry biochemical sensing device and its method for sensing, this device is designed to the mode of one in conjunction with blood sampling design, test piece design and sensing instrument, in blood sampling, finish the work that multiple physiological parameter detects, the user is provided the more convenient service that has more value.But and the invention provides the window that continuous several times detects, and need not carry plurality of devices simultaneously during use, as blood taking needle and check test paper etc., increased the convenience that uses.
For reaching above-mentioned purpose, the technology used in the present invention means are as follows, and a kind of integrated galvanochemistry biochemical sensing device comprises:
At least one sample collecting apparatus, utilize the sample bearing space in this sample collecting apparatus and utilize external force to cooperate the mode of pressure differential to make inner vacuum or the negative pressure state of forming of this sample bearing space, can enter sample bearing space inside rapidly in order to the body fluid example to be measured of relatively high pressure;
At least one electrode group, comprise a working electrode, a reference electrode and an auxiliary electrode, after this body fluid example to be measured entered sample bearing space inside, this working electrode and the chemical substance on it can be carried out chemistry and physical reactions with body fluid example to be measured;
One arithmetic logic and data fatching apparatus carried out the acquisition and the computing of data with carrying out the result of chemistry with physical reactions;
One signal display apparatus, with show clearly following each: the physiological parameter numerical value of body fluid example to be measured, the corresponding concentration of physiological parameter.
The invention provides a kind of integrated galvanochemistry biochemical sensing method, comprising:
(1) utilize the sample bearing space in the sample harvester to collect body fluid example to be measured, promptly utilize external force to cooperate the mode of pressure differential to make inner vacuum or the negative pressure state of forming of this sample bearing space, can enter sample bearing space inside rapidly in order to this body fluid example to be measured of relatively high pressure;
(2) utilize in the plurality of electrodes group at least one electrode group that body fluid example to be measured is carried out chemistry and physical reactions, and each electrode group comprises a working electrode, a reference electrode and an auxiliary electrode, promptly after this body fluid example to be measured entered sample bearing space inside, this working electrode and the chemical substance on it can be carried out chemistry and physical reactions with body fluid example to be measured;
(3) utilize acquisition of an arithmetic logic and data fatching apparatus and the reacted result of computing, carried out the acquisition and the computing of data carrying out the result of chemistry with physical reactions.
Blood sampling mode of the present invention is to utilize the mode of pressure differential, vacuum or negative pressure state in the surveyed area, when the user pushes at surveyed area to detect the position, miniature blood-taking device in the surveyed area just can the penetration-detection position skin, and owing to be the state of vacuum or negative pressure in the surveyed area, therefore, blood is just promptly via blood-taking device institute transdermal part, be full of the space of surveyed area, treat that the back of internal and external pressure balance promptly stops, so can reaching accurately and detect the quantitative purpose of liquid.
Characteristic of the present invention is integrated galvanochemistry biochemical sensing device, and this device comprises blood sampling, sensing, signal output and has continuous detecting need not change advantages such as accessory.The product that is had on the market at present, all with blood-taking device, the mode that sensing test piece separates with sensing apparatus is handled, and is indispensable during use, and the user must repeat blood sampling, get actions such as blood, sensing, can carry out repeatedly or different testings.Therefore, the present invention integrates above-mentioned standalone object, but makes this integrated galvanochemistry biochemical sensing device have the advantage that simple to operate, easy to carry and continuous detecting need not changed accessory.
For making the personage who is familiar with this skill understand purpose of the present invention, feature and effect, now by following specific embodiment, and accompanying drawing shown in cooperating, the present invention is described as follows in detail.
Description of drawings
Fig. 1 is the stereographic map of a preferred embodiment of the present invention;
Fig. 2 is the side elevation view of preferred embodiment of the present invention;
Fig. 3 is the drawings in detail of an electrode group;
Fig. 4 A is another embodiment synoptic diagram of sample collecting apparatus of the present invention;
Fig. 4 B is an embodiment synoptic diagram again of sample collecting apparatus of the present invention;
Fig. 5 is the another embodiment synoptic diagram of sample collecting apparatus of the present invention.
Fig. 6 is a method for sensing step of the present invention.
Symbol description among the figure:
1 sensing apparatus
11 sample collecting apparatus
111 shells
113 sample bearing spaces
115 spicules
117 sample collectings point
119 is small holes
12 buttons
13 electrode groups
131 auxiliary electrodes
132 working electrodes
133 reference electrodes
14 multi-channel electrochemical sensing apparatus
141 leads
142 leads
15 fingers
16 arithmetic logics and data fatching apparatus
161 leads
17 body fluid examples to be measured
21 sample collecting apparatus
211 shells
213 sample bearing spaces
215 protrusions
31 sample collecting apparatus
311 shells
313 sample bearing spaces
315 protrusions
317 little rubber balls
41 sample collecting apparatus
411 shells
413 sample bearing spaces
415 protrusions
Embodiment
See also Fig. 1, the stereographic map of a preferred embodiment of the present invention, show a sensing apparatus 1 of the present invention the front-operated panel each partly, comprise 28 groups of sample collecting apparatus 11, a signal display apparatus 19 and five buttons 12.Each group sample collecting apparatus 11 all cooperates an electrode group 13, when pressing the button 12 driving sample collecting apparatus 11, promptly begin to carry out the collection of body fluid example to be measured, and carry out chemistry and physical reactions with sample room by this electrode group 13, effect through a multi-channel electrochemical sensing apparatus (not shown) and an arithmetic logic and data fatching apparatus (not shown) will the results are shown in this signal display apparatus 19.These a plurality of buttons 12 are pressed the several seconds and after not unclamping or pressing different buttons, are promptly shown record, the different functions such as measurement project of conversion that time, preceding several are measured.The physiological parameter that this a plurality of surveyed area detected can be adjusted with different product designs simultaneously, does not limit to detect single physiological parameter.
See also Fig. 2 and Fig. 3, the drawings in detail of the side elevation view of preferred embodiment of the present invention and an electrode group.Wherein, a kind of integrated galvanochemistry biochemical sensing device of the present invention comprises:
A plurality of sample collecting apparatus 11, utilize the sample bearing space 113 in this sample collecting apparatus, and utilize external force to cooperate the mode of pressure differential to make inner vacuum or negative pressure state (the genus known technology of forming of this sample bearing space, seldom inquire at this), body fluid example to be measured 17 (the 2nd figure shows from the blood in the finger 15) in order to relatively high pressure can enter sample bearing space inside 113 rapidly, and have more a spicule 115 in this sample collecting apparatus 11, and this spicule 115 can be broken through a shell 111 of this sample bearing space 113, and can on a sample collection point 117, produce a small hole 119, enter in order to body fluid example 17 to be measured;
Plurality of electrodes group 13, each electrode group 13 comprises a working electrode 132, one reference electrode 133 and an auxiliary electrode 131, after this body fluid example 17 to be measured enters sample bearing space 113 inside, this working electrode 132 can carry out chemistry and physical reactions with body fluid example to be measured with the chemical substance on it, and so-called first kind of chemical reaction is to utilize the ferment molecule and the body fluid example to be measured 17 that contain on the workplace of this working electrode 132 to produce reaction, with the electron transfer mediator of oxygen molecule as biochemical reaction, and first kind of working electrode refers to that this is through ferment molecule and the suitable cross-linking reagent institute electrode of modification jointly, wherein, this ferment molecule is the redox ferment, comprise: the glucose oxidase ferment, glucose dehydrogenation ferment, cholesterol oxidation ferment, similar chemical substance, and this cross-linking reagent can be many aldehyde radicals macromolecular compound, many aldehyde radicals macromolecule natural goods, the compound that contains the dialdehyde base, polyamines based high molecular compound, many aldehyde radicals macromolecule natural goods, the compound that contains the diamine base, many carboxyls macromolecular compound, many carboxyls macromolecule natural goods, two carboxyl molecular compounds or similar chemical substance; And second kind of chemical reaction that this chemical substance and body fluid example to be measured 17 carry out is to utilize ferment molecule and electron transfer mediator and the body fluid example to be measured generation reaction that contains on the workplace of this working electrode 132, and second kind of working electrode refers to that this is through ferment molecule and a suitable cross-linking reagent and the suitable electron transfer mediator institute electrode of modification jointly, this electron transfer mediator comprises electron accepter, electronics gives body, catalytic reagent, on the other hand, it is made by the high conductivity inks that contains carbon printing ink or other metal ink that physical reactions utilizes working electrode 132, pass to suitable and enough operating voltages again, electron transport in order to chemical reaction, this reference electrode 133 can be used as the relative pointer of operating voltage that this working electrode applies, comprise that silver/silver chloride reference electrode or saturated calomel electrode or other can be used as the material of reference electrode, these auxiliary electrode 131 its materials can be made by high conductivity inks that contains carbon printing ink or other metal ink or similar metal;
One multi-channel electrochemical sensing apparatus 14, can provide a plurality of operating voltage also by a plurality of working electrodes 132 in a plurality of electric wire 141 connection electrode groups 13, this multi-channel electrochemical sensing apparatus 14 can suitably be adjusted along with the difference of inspected object or electron transfer mediator;
One arithmetic logic and data fatching apparatus 16, the result that will carry out chemistry and physical reactions by an electric wire 142 carries out the acquisition and the computing of data;
One signal display apparatus 19 with the data after 16 acquisitions of this arithmetic logic and data fatching apparatus and the computing, shows the physiological parameter numerical value or the corresponding concentration of physiological parameter of body fluid example 17 to be measured by an electric wire 161.
Yet, in the present embodiment, working electrode in the plurality of electrodes group has two kinds, promptly first kind can be passed through to modify different ferment molecule and cross-linking reagent, reach the check of multi-physiological-parameter, second kind can be by modifying different ferment molecule and cross-linking reagent and electron transfer mediator, the check of reaching multi-physiological-parameter.
With the glucose sensing is example, and the synoptic diagram of above-mentioned biomolecular reaction and electron transfer is as follows:
Generally speaking, nature is used as the required electron accepter of ferment biochemical reaction with oxygen molecule, and the synoptic diagram of its electron transfer is as follows:
Therefore, the consumption that can throughput oxygen determination atmosphere or the growing amount of Liquor Hydrogen Peroxide molecule reach the purpose of quantitative concentration of glucose.Electron accepter also can be other chemical substance, as: Ferrocene, K
3Fe (CN)
6, TTF-FCNQ or the like, with K
3Fe (CN)
6Be example, the synoptic diagram of its electron transfer is as follows:
By measuring Fe (CN)
6 3-/ Fe (CN)
6 4-Electron transfer reach the purpose of quantitative concentration of glucose.
In addition, also can utilize the growing amount that detects Liquor Hydrogen Peroxide to reach quantitative purpose, for example utilize Prussian blue crystal to be used as the catalyzer of Liquor Hydrogen Peroxide, the synoptic diagram of its electron transfer is as follows:
This mechanism is come quantitative concentration of glucose by the electron transfer of Liquor Hydrogen Peroxide reduction reaction gained.Other electrode group is then carried out different testings, detection selectivity between the different electrode groups, then be to do discriminating by ferment or other biochemical molecular fixed on the working electrode, survey species and give birth to selectivity and the selectivity that tool is had for band by biomolecule, the selectivity of reaching various physiological parameter detects.
In addition, multi-channel electrochemical detection system of the present invention is between the different electrode groups, different operating voltage can be provided respectively, and these operating voltage can be adjusted to some extent along with different electron transfer mediators or in order to distinguish different physiological detection parameters, and because the different concentration of physiological detection parameter in health and inequality, and biomolecule fundamental characteristics corresponding with it is also inequality, therefore in the system also at the different logical operation mode of design between the different electrode groups, can independently calculate and analyze the signal after the physiological detection, be presented on the display by electronic circuit again.
With Fig. 3, electrode group 13 has four working electrodes 132, four auxiliary electrodes 131 and reference electrodes 133.Therefore as can be known, after this body fluid example to be measured is collected,,, can carry out the analysis of four kinds of physiological parameters so once collect because four groups of working electrodes, auxiliary electrode and reference electrodes are arranged.
With Fig. 1 is example, has 28 groups of sample collecting apparatus 11, almost is one month trace detection so can schedule to last at these four kinds of physiological parameters.On the other hand, this galvanochemistry biochemical sensing device can apply different operating voltage simultaneously in different working electrode groups, or can not apply different operating voltage simultaneously in different working electrode groups.And the different voltages that this working electrode applied are adjustable according to different electron transfer mediators and different.And the different physiological parameters that electrode detected, can adjust with the design of product is different, do not limit and detect single physiological parameter.
See also Fig. 4 A, another embodiment synoptic diagram of sample collecting apparatus of the present invention.Produce a small protrusion 215 through particular design on the shell 211 of the sample bearing space 213 of this sample collecting apparatus 21, on it and a small outstanding aperture arranged, this protrusion 215 can (as the somewhere on the finger) be gone up generation one small hole (as a little blood hole) in a sample collection point, can sample be penetrated in the sample bearing space 213 by this small outstanding aperture during collection.See also Fig. 4 B, an embodiment synoptic diagram again of sample collecting apparatus of the present invention.Produce a needle-like protrusion 415 through particular design in the shell 411 of the sample bearing space 413 of this sample collecting apparatus 41, this protrusion 415 can penetrate this shell 411, and on a sample collection point (as the somewhere on the finger), produce small hole (as a little blood hole), can be during collection by this small hole and shell 411 interior relative negative pressure or vacuum state, and make sample penetrate in the sample bearing space 413.
See also Fig. 5, the another embodiment synoptic diagram of sample collecting apparatus of the present invention.Produce a spicule 315 through particular design on the shell 311 of the sample bearing space 313 of this sample collecting apparatus 31, on it and a small outstanding aperture arranged, and be used as the function of gas-tight seal with a small ball, keeping vacuum or negative pressure state in the sample bearing space, and can sample be penetrated in the sample bearing space 313 by this small outstanding aperture when gathering.
See also Fig. 6 and be method for sensing step of the present invention.A kind of integrated galvanochemistry biochemical sensing method of the present invention comprises:
(1) utilize the sample bearing space in the sample harvester to collect body fluid example to be measured, promptly this sample bearing space inside is vacuum or relative negative pressure state, utilize external force to cooperate the mode of pressure differential, can enter sample bearing space inside rapidly in order to this body fluid example to be measured of relatively high pressure; Wherein, more can utilize following three kinds of embodiment to obtain sample, can utilize a spicule of this sample collecting apparatus, and this spicule can break through a shell of this sample bearing space, and can on a sample collection point, produce a small hole; Maybe can utilize on this sample bearing space shell and produce a small protrusion through particular design, this protrusion can produce a small hole on a sample collection point; Maybe can utilize on this sample bearing space shell through particular design and produce a small outstanding aperture, and be used as the function of gas-tight seal, to keep vacuum or negative pressure state in the sample bearing space with a small ball;
(2) utilize in the plurality of electrodes group at least one electrode group that body fluid example to be measured is carried out chemistry and physical reactions, and each electrode group comprises a working electrode, a reference electrode and an auxiliary electrode, promptly after this body fluid example to be measured entered sample bearing space inside, this working electrode and the chemical substance on it can be carried out chemistry and physical reactions with body fluid example to be measured; And first kind of chemical reaction utilizes the ferment molecule and the body fluid example to be measured that contain on the workplace of this working electrode to produce reaction, with the electron transfer mediator of oxygen molecule as biochemical reaction, and working electrode refers to that this is through ferment molecule and the suitable cross-linking reagent institute electrode of modification jointly, wherein, this ferment molecule is the redox ferment, comprise: the glucose oxidase ferment, glucose dehydrogenation ferment, cholesterol oxidation ferment or similar chemical substance, this cross-linking reagent can be many aldehyde radicals macromolecular compound, many aldehyde radicals macromolecule natural goods, the dialdehyde based compound, polyamines based high molecular compound, polyamines based high molecular natural goods, the diamine based compound, many carboxyls macromolecular compound, many carboxyls macromolecule natural goods, two carboxyl compounds, or similar chemical substance; Second kind of chemical reaction utilizes the ferment molecule that contains on the workplace of this working electrode and a suitable cross-linking reagent and suitable electron transfer mediator and body fluid example to be measured to produce reaction, and working electrode refers to that this is through ferment molecule and a suitable cross-linking reagent and the suitable electron transfer mediator institute electrode of modification jointly; The physical reactions that this working electrode and body fluid example to be measured carry out utilizes working electrode made by the high conductivity inks that contains carbon printing ink or other metal ink, passes to suitable and enough operating voltages again, in order to the electron transport of chemical reaction; This electrode group more can comprise a multi-channel electrochemical sensing apparatus, can provide at least one operating voltage to this working electrode; This reference electrode can be used as the relative pointer of operating voltage that this working electrode applies, and comprises that silver/silver chloride reference electrode, saturated calomel electrode or other can be used as the material of reference electrode; This auxiliary electrical extremely material is made by high conductivity inks that contains carbon printing ink or other metal ink or similar metal;
(3) utilize acquisition of an arithmetic logic and data fatching apparatus and the reacted result of computing, carried out the acquisition and the computing of data carrying out the result of chemistry with physical reactions.
(4) utilize a signal display apparatus, to show the corresponding concentration of physiological parameter numerical value, physiological parameter of body fluid example to be measured clearly.
Though the present invention discloses as above with a plurality of preferred embodiments; right its is not in order to limit the present invention; be that all equalizations of doing according to claim of the present invention change and modify etc.; all should still belong to patent covering scope of the present invention, protection scope of the present invention is as the criterion with the described claim person of defining.
Claims (37)
1. integrated galvanochemistry biochemical sensing device comprises:
At least one sample collecting apparatus, utilize the sample bearing space in this sample collecting apparatus, and utilize external force to cooperate the mode of pressure differential to make inner vacuum or the negative pressure state of forming of this sample bearing space, can enter sample bearing space inside rapidly in order to the body fluid example to be measured of relatively high pressure;
At least one electrode group, comprise a working electrode, a reference electrode and an auxiliary electrode, after this body fluid example to be measured entered sample bearing space inside, this working electrode and the chemical substance on it can be carried out chemistry and physical reactions with body fluid example to be measured;
One arithmetic logic and data fatching apparatus carried out the acquisition and the computing of data with carrying out the result of chemistry with physical reactions.
2. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, this device more comprises a signal display apparatus, with show clearly following each: the physiological parameter numerical value of body fluid example to be measured, the corresponding concentration of physiological parameter.
3. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, have more a spicule in this sample collecting apparatus, and this spicule a shell of this sample bearing space can be broken through, and a small hole can be on a sample collection point, produced.
4. a kind of according to claim 1 integrated galvanochemistry biochemical sensing device wherein, produces a small protrusion through particular design on this sample bearing space shell, and this protrusion can produce a small hole on a sample collection point.
5. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, produce a small outstanding aperture through particular design on this sample bearing space shell, and be used as the function of gas-tight seal, to keep vacuum or negative pressure state in the sample bearing space with a small ball.
6. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, this chemical substance and body fluid example to be measured carry out chemical reaction, be to utilize the ferment molecule and the body fluid example to be measured that contain on the workplace of this working electrode to produce reaction, with the electron transfer mediator of oxygen molecule as biochemical reaction, and working electrode refers to that this is through ferment molecule and the suitable cross-linking reagent institute electrode of modification jointly.
7. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, the chemical reaction that this chemical substance and body fluid example to be measured carry out utilizes the ferment molecule that contains on one of this working electrode workplace and electron transfer mediator and body fluid example to be measured to produce reaction, and working electrode refers to that this is through ferment molecule and a suitable cross-linking reagent and the suitable electron transfer mediator institute electrode of modification jointly.
8. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, the physical reactions that this working electrode and body fluid example to be measured carry out utilizes working electrode made by the high conductivity inks that contains carbon printing ink or other conductive metal printing ink, pass to suitable and enough operating voltages again, in order to the electron transport of chemical reaction.
9. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, this electrode group more can comprise a multi-channel electrochemical sensing apparatus, can provide at least one operating voltage to this working electrode.
10. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, this reference electrode can be used as the relative pointer of operating voltage that this working electrode applies, comprise following each: silver/silver chloride reference electrode, saturated calomel electrode, other can be used as the material of reference electrode.
11. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 1, wherein, this auxiliary electrical extremely material is that to contain the high conductivity inks of carbon printing ink or other conductive metal printing ink made, comprising: carbon, gold, platinum, similar metal.
12. an integrated galvanochemistry biochemical sensing device comprises:
At least one sample collecting apparatus, utilize the sample bearing space in this sample collecting apparatus, and utilize external force to cooperate the mode of pressure differential to make inner vacuum or the negative pressure state of forming of this sample bearing space, can enter sample bearing space inside rapidly in order to the body fluid example to be measured of relatively high pressure;
The plurality of electrodes group, each electrode group comprises a working electrode, a reference electrode and an auxiliary electrode, after this body fluid example to be measured entered sample bearing space inside, this working electrode and the chemical substance on it can be carried out chemistry and physical reactions with body fluid example to be measured;
One multi-channel electrochemical sensing apparatus can provide a plurality of operating voltage to these a plurality of working electrodes;
One arithmetic logic and data fatching apparatus carried out the acquisition and the computing of data with carrying out the result of chemistry with physical reactions.
13. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, this device more comprises a signal display apparatus, with show clearly following each: the physiological parameter numerical value of body fluid example to be measured, the corresponding concentration of physiological parameter.
14. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, have more a spicule in this sample collecting apparatus, and this spicule one of this sample bearing space shell can be broken through, and a small hole can be on a sample collection point, produced.
15. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12 wherein, produces a small protrusion through particular design on this sample bearing space shell, this protrusion can produce a small hole on a sample collection point.
16. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, produce a small outstanding aperture through particular design on this sample bearing space shell, and be used as the function of gas-tight seal, to keep vacuum or negative pressure state in the sample bearing space with a small ball.
17. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, the chemical reaction that this chemical substance and body fluid example to be measured carry out utilizes the ferment molecule and the body fluid example to be measured that contain on the workplace of this working electrode to produce reaction, with the electron transfer mediator of oxygen molecule as biochemical reaction, and working electrode refers to that this is through ferment molecule and the suitable cross-linking reagent institute electrode of modification jointly.
18. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, the chemical reaction that this chemical substance and body fluid example to be measured carry out utilizes ferment molecule and electronics and the body fluid example to be measured generation reaction that contains on the workplace of this working electrode, transmitting medium, and working electrode refers to that this is through ferment molecule and a suitable cross-linking reagent and the suitable electron transfer mediator institute electrode of modification jointly.
19. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, the physical reactions that this working electrode and body fluid example to be measured carry out utilizes working electrode made by the high conductivity inks that contains carbon printing ink or other conductive metal printing ink, pass to suitable and enough operating voltages again, in order to the electron transport of chemical reaction.
20. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, this multi-channel electrochemical sensing apparatus can suitably be adjusted along with the difference that detects species or electron transfer mediator.
21. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, this reference electrode can be used as the relative pointer of operating voltage that this working electrode applies, comprise following each: silver/silver chloride reference electrode, saturated calomel electrode, other can be used as the material of reference electrode.
22. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, this auxiliary electrical extremely material is that to contain the high conductivity inks of carbon printing ink or other conductive metal printing ink made, comprising: carbon, gold, platinum, similar metal.
23. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, the working electrode in this plurality of electrodes group can be by modifying different ferment molecule and cross-linking reagent, the check of reaching multi-physiological-parameter.
24. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, the working electrode in this plurality of electrodes group can be by modifying different ferment molecule and cross-linking reagent and electron transfer mediator, the check of reaching multi-physiological-parameter.
25. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, this galvanochemistry biochemical sensing device can apply different operating voltage simultaneously in different working electrode groups.
26. a kind of integrated galvanochemistry biochemical sensing device as claimed in claim 12, wherein, this galvanochemistry biochemical sensing device can not apply different operating voltage simultaneously in different working electrode groups.
27. an integrated galvanochemistry biochemical sensing method comprises:
(1) utilize the sample bearing space in the sample harvester to collect body fluid example to be measured, promptly utilize external force to cooperate the mode of pressure differential to make inner vacuum or the negative pressure state of forming of this sample bearing space, can enter sample bearing space inside rapidly in order to this body fluid example to be measured of relatively high pressure;
(2) utilize in the plurality of electrodes group at least one electrode group that body fluid example to be measured is carried out chemistry and physical reactions, and each electrode group comprises a working electrode, a reference electrode and an auxiliary electrode, promptly after this body fluid example to be measured entered sample bearing space inside, this working electrode and the chemical substance on it can be carried out chemistry and physical reactions with body fluid example to be measured;
(3) utilize acquisition of an arithmetic logic and data fatching apparatus and the reacted result of computing, carried out the acquisition and the computing of data carrying out the result of chemistry with physical reactions.
28. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, after step (3), more can utilize a signal display apparatus, with show clearly following each: the physiological parameter numerical value of body fluid example to be measured, the corresponding concentration of physiological parameter.
29. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, in step (1), can utilize a spicule of this sample collecting apparatus, and this spicule a shell of this sample bearing space can be broken through, and a small hole can be on a sample collection point, produced.
30. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, can utilize in step (1) on this sample bearing space shell and produce a small protrusion through particular design, this protrusion can produce a small hole on a sample collection point.
31. as a kind of integrated galvanochemistry biochemical sensing method as described in the claim 27, wherein, in step (1), can utilize on this sample bearing space shell and produce a small outstanding aperture through particular design, and be used as the function of gas-tight seal with a small ball, to keep vacuum or negative pressure state in the sample bearing space.
32. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, the chemical reaction that this chemical substance and body fluid example to be measured carry out in step (2) utilizes the ferment molecule and the body fluid example to be measured that contain on the workplace of this working electrode to produce reaction, with the electron transfer mediator of oxygen molecule as biochemical reaction, and working electrode refers to that this is through ferment molecule and the suitable cross-linking reagent institute electrode of modification jointly.
33. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, the chemical reaction that this chemical substance and body fluid example to be measured carry out in step (2) utilizes ferment molecule and electronics and the body fluid example to be measured generation reaction that contains on the workplace of this working electrode, transmitting medium, and working electrode refers to that this is through ferment molecule and a suitable cross-linking reagent and the suitable electron transfer mediator institute electrode of modification jointly.
34. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, the physical reactions that this working electrode and body fluid example to be measured carry out in step (2) utilizes working electrode made by the high conductivity inks that contains carbon printing ink or other conductive metal printing ink, pass to suitable and enough operating voltages again, in order to the electron transport of chemical reaction.
35. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, this electrode group more can comprise a multi-channel electrochemical sensing apparatus in step (2), can provide at least one operating voltage to this working electrode.
36. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, this reference electrode can be used as the relative pointer of operating voltage that this working electrode applies in step (2), comprise following each: silver/silver chloride reference electrode, saturated calomel electrode, other can be used as the material of reference electrode.
37. a kind of integrated galvanochemistry biochemical sensing method as claimed in claim 27, wherein, in step (2) this auxiliary electrical extremely material be that to contain the high conductivity inks of carbon printing ink or other conductive metal printing ink made, comprising: carbon, gold, platinum, similar metal.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102004126A (en) * | 2010-05-11 | 2011-04-06 | 中国科学院上海应用物理研究所 | Electrochemical biosensor and detecting method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102004126A (en) * | 2010-05-11 | 2011-04-06 | 中国科学院上海应用物理研究所 | Electrochemical biosensor and detecting method thereof |
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