CN1629174A - Puerarin preparing process - Google Patents
Puerarin preparing process Download PDFInfo
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- CN1629174A CN1629174A CN 200410035624 CN200410035624A CN1629174A CN 1629174 A CN1629174 A CN 1629174A CN 200410035624 CN200410035624 CN 200410035624 CN 200410035624 A CN200410035624 A CN 200410035624A CN 1629174 A CN1629174 A CN 1629174A
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- puerarin
- preparing
- elutriant
- water
- crystallization
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Abstract
The invention relates to a process for preparing puerarin by using water as dissolvent for extraction, the process consists of isolating the filtered raffinate through passing chromatography column, concentrating the eluent by means of membrane separation technique, finally crystallizing and refining to obtain the pure product. The advantages of the invention include simple preparation process, easiness in operation, shorter production circle, low energy consumption, and high efficiency.
Description
Technical field
The present invention relates to a kind of method for preparing puerarin, be the traditional Chinese medicine extraction separation technology field.
Background technology
Puerarin is the effective constituent that is obtained by extraction separation in the Chinese medicine elegant jessamine, has the coronary artery dilator and the cerebrovascular, reduces myocardial consumption of oxygen, improves the effect of blood circulation and anti-platelet aggregation.Be used for treating coronary heart disease, stenocardia, myocardial infarction, retina arteriovenous obstruction and sudden deafness clinically.
Extract the method for puerarin from the Chinese medicine elegant jessamine, the water that has commonly used is carried and alcohol extracting, and it generally is to carry out alcohol precipitation again after extracting solution is concentrated that water is carried, and reclaims alcohol, obtains medicinal extract; Shortcomings such as alcohol extracting obtains medicinal extract after also needing to reclaim alcohol, carries out chromatographic separation again, uses a large amount of solvent wash-outs then, and all lifetime is long for two kinds of methods, and energy consumption is big, and the process complexity is loaded down with trivial details, and equipment is many.Especially concentrating very a large amount of elutriants needs many large-scale thickening equipments, consumes a large amount of steam, has increased the manufacturing cost of puerarin.Because puerarin is heated in solvent for a long time, has influenced the quality of product.
Summary of the invention
Technical problem to be solved by this invention is: improve the weak point of prior art, a kind of method for preparing puerarin is provided, this method technological process is simple, easy to operate, with short production cycle, energy consumption is low, the efficient height, equipment is few, comprehensive low cost of manufacture, constant product quality.
The method for preparing puerarin of the present invention is that employing water is that solvent extracts, and extracting solution is without concentrating, filter the back and directly go up the chromatography column separation, to detect the elutriant of collecting the back with thin layer and adopt membrane separation technique to concentrate, concentrated solution through crystallization, refining, obtains the pure product of puerarin more at last.
Method of the present invention adopts extracting solution directly to go up the chromatography column separation, needn't concentrate extracting solution, has saved thickening equipment, has simplified operation, has saved a large amount of steam, has reduced energy consumption, has shortened the time.
Elutriant concentration method of the present invention adopts membrane separation technique to concentrate, and has shortened concentration time greatly, reduced energy consumption, saved many equipment, adopted membrane separation technique to concentrate simultaneously, avoided conventional when concentrated puerarin be heated for a long time and influence the drawback of quality.
Chromatographic separating process of the present invention, the sorbent material that is adopted can be polymeric adsorbents such as polymeric amide, macroporous resin, dextrane gel.
Chromatographic separating process of the present invention, the elutriant that is adopted can be pure water or the pure water that contains alcohol.
The routine operation that the present invention relates to is a known technology.
Compared with the prior art the method for preparing puerarin of the present invention has following distinguishing feature and positively effect: extracting solution of the present invention is directly gone up the chromatography column separation method without concentrating, and the time shortens 60%, and energy consumption saves 80%.Elutriant concentration method of the present invention, the equipment input saves 70%, and running cost has only 20% of ordinary method.
Owing to adopted the direct upper prop of extracting solution to separate and with the method for membrane separation technique concentrate eluant, make the present invention prepare the method for puerarin, it is simple to have technological process, easy to operate, with short production cycle, energy consumption is low, the equipment less investment, comprehensive low cost of manufacture, characteristics such as constant product quality, its remarkable benefit is beneficial to suitability for industrialized production and uses.The inventive method may be used in the extraction separation of other Chinese medicine, and the especially application of membrane separation technique has positive effect to promoting the modernization of Chinese medicine.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
The method for preparing puerarin of the present invention is carried out according to the following steps:
(1) extracts
Treated root of kudzu vine 400Kg is added in the extractor, add the water of 4-5 times of medicinal material weight, heating keeps little boiling 4 hours, and blowing repeats 3 times, merges whole extracting solutions.
(2) column chromatography
Separate going up chromatography column behind the extracting liquid filtering, detect the back with thin layer and collect the elutriant that contains puerarin.
(3) elutriant concentrates
The elutriant of collecting is passed through membrane concentration equipment, obtain containing the concentrated solution of puerarin 4-8%.
(4) crystallization, refining
Concentrated solution low temperature is placed, is had crystallization to separate out, filter crystallization, again through refining pure product of puerarin that obtain repeatedly.
Claims (9)
1, a kind of method for preparing puerarin, it is characterized in that adopting water is that solvent extracts, extracting solution is without concentrating, filter the back and directly wash the post separation in the upper strata, to detect the elutriant of collecting the back with thin layer adopts membrane separation technique to concentrate, concentrated solution through crystallization, refining, obtains the pure product of puerarin more at last.
2, the method for preparing puerarin according to claim 1, it is characterized in that extracting used solvent is water.
3, the method for preparing puerarin according to claim 1 is characterized in that extracting solution without concentrating, and filters the back and directly goes up the chromatography column separation.
4, the method for preparing puerarin according to claim 3 is characterized in that the elutriant that chromatography is used can be pure water or the water that contains alcohol.
5, the method for preparing puerarin according to claim 4 is characterized in that the elutriant that contains puerarin that obtains after the chromatographic separation adopts membrane separation technique to concentrate.
6, the method for preparing puerarin according to claim 4 is characterized in that the resin that chromatography is used can be polymeric amide, macroporous resin, dextrane gel resin.
7, the method for preparing puerarin according to claim 5 is characterized in that the detection method to puerarin in the elutriant is that thin layer detects.
8, the method for preparing puerarin according to claim 5, the concentration that it is characterized in that puerarin in the concentrated solution is 4-8%.
9, the method for preparing puerarin according to claim 8 is characterized in that concentrated solution low temperature is placed, and separates out crystallization, filter crystallization, again through the refining pure product of puerarin that obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100356243A CN1312168C (en) | 2004-08-29 | 2004-08-29 | Puerarin preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100356243A CN1312168C (en) | 2004-08-29 | 2004-08-29 | Puerarin preparing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1629174A true CN1629174A (en) | 2005-06-22 |
| CN1312168C CN1312168C (en) | 2007-04-25 |
Family
ID=34845723
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100356243A Expired - Fee Related CN1312168C (en) | 2004-08-29 | 2004-08-29 | Puerarin preparing process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1312168C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355766C (en) * | 2005-10-21 | 2007-12-19 | 安徽农业大学 | Method for preparing and purifying uperarin through membrane technology |
| CN102532111A (en) * | 2010-12-24 | 2012-07-04 | 天津中新药业集团股份有限公司第六中药厂 | Method for extracting puerarin from traditional Chinese medicine kudzu |
| CN103131578A (en) * | 2011-11-29 | 2013-06-05 | 中润益源(北京)生物科技有限公司 | Kudzuvine root comprehensive processing and utilization scheme |
| CN103789366A (en) * | 2014-02-21 | 2014-05-14 | 王健鹏 | Method for extracting puerarin through compound enzyme hydrolysis |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1057093C (en) * | 1995-02-23 | 2000-10-04 | 赵爱平 | Extraction and application of Lobed Kudzuvine |
| CN1393248A (en) * | 2001-07-03 | 2003-01-29 | 买凯 | Extracting process and application of active component of pueraria root |
| CN1162421C (en) * | 2002-01-28 | 2004-08-18 | 湖南省强生药业有限公司 | New process for extracting high purity puerarin by pure water method |
| CN1483734A (en) * | 2003-07-26 | 2004-03-24 | 卓宇哲 | Novel process for extracting puerarin from pueraria mirifica |
-
2004
- 2004-08-29 CN CNB2004100356243A patent/CN1312168C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355766C (en) * | 2005-10-21 | 2007-12-19 | 安徽农业大学 | Method for preparing and purifying uperarin through membrane technology |
| CN102532111A (en) * | 2010-12-24 | 2012-07-04 | 天津中新药业集团股份有限公司第六中药厂 | Method for extracting puerarin from traditional Chinese medicine kudzu |
| CN102532111B (en) * | 2010-12-24 | 2016-04-27 | 天津中新药业集团股份有限公司第六中药厂 | A kind of method extracting puerarin from Chinese medicine elegant jessamine |
| CN103131578A (en) * | 2011-11-29 | 2013-06-05 | 中润益源(北京)生物科技有限公司 | Kudzuvine root comprehensive processing and utilization scheme |
| CN103131578B (en) * | 2011-11-29 | 2016-04-27 | 中润益源(北京)生物科技有限公司 | A kind of scheme of root of kudzu vine comprehensive processing and utilization |
| CN103789366A (en) * | 2014-02-21 | 2014-05-14 | 王健鹏 | Method for extracting puerarin through compound enzyme hydrolysis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1312168C (en) | 2007-04-25 |
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