CN1312168C - Puerarin preparing process - Google Patents

Puerarin preparing process Download PDF

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Publication number
CN1312168C
CN1312168C CNB2004100356243A CN200410035624A CN1312168C CN 1312168 C CN1312168 C CN 1312168C CN B2004100356243 A CNB2004100356243 A CN B2004100356243A CN 200410035624 A CN200410035624 A CN 200410035624A CN 1312168 C CN1312168 C CN 1312168C
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China
Prior art keywords
puerarin
preparing
pure
crystallization
concentrated solution
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Expired - Fee Related
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CNB2004100356243A
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CN1629174A (en
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徐卫臣
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徐卫臣
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Abstract

The present invention relates to a method for preparing puerarin, which belongs to the technical field of extraction and separation of a traditional Chinese medicine. In the method, water is adopted as a dissolvant for extracting puerarin; after being filtered, an extraction liquid is directly separated by a chromatography column; an eluant is concentrated by a membrane separation technique, crystallized and refined to obtain a pure puerarin product. The method has the characteristics of simple technological process, convenient operation, short production periodicity, low energy consumption, high efficiency, less equipment investment, low cost of comprehensive manufacture and stable product quality.

Description

A kind of method for preparing puerarin
Technical field
The present invention relates to a kind of method for preparing puerarin, be the traditional Chinese medicine extraction separation technology field.
Background technology
Puerarin is the effective constituent that is obtained by extraction separation in the Chinese medicine elegant jessamine, has the coronary artery dilator and the cerebrovascular, reduces myocardial consumption of oxygen, improves the effect of blood circulation and anti-platelet aggregation.Be used for treating coronary heart disease, stenocardia, myocardial infarction, retina arteriovenous obstruction and sudden deafness clinically.
Extract the method for puerarin from the Chinese medicine elegant jessamine, the water that has commonly used is carried and alcohol extracting, and it generally is to carry out alcohol precipitation again after extracting solution is concentrated that water is carried, and reclaims alcohol, obtains medicinal extract; Shortcomings such as alcohol extracting obtains medicinal extract after also needing to reclaim alcohol, carries out chromatographic separation again, uses a large amount of solvent wash-outs then, and all lifetime is long for two kinds of methods, and energy consumption is big, and the process complexity is loaded down with trivial details, and equipment is many.Especially concentrating very a large amount of elutriants needs many large-scale thickening equipments, consumes a large amount of steam, has increased the manufacturing cost of puerarin.Because puerarin is heated in solvent for a long time, has influenced the quality of product.
Summary of the invention
Technical problem to be solved by this invention is: improve the weak point of prior art, a kind of method for preparing puerarin is provided, this method technological process is simple, easy to operate, with short production cycle, energy consumption is low, the efficient height, equipment is few, comprehensive low cost of manufacture, constant product quality.
The method for preparing puerarin of the present invention is that employing water is that solvent extracts, and extracting solution is without concentrating, filter the back and directly go up the chromatography column separation, to detect the elutriant of collecting the back with thin layer and adopt membrane separation technique to concentrate, concentrated solution through crystallization, refining, obtains the pure product of puerarin more at last.
Method of the present invention adopts extracting solution directly to go up the chromatography column separation, needn't concentrate extracting solution, has saved thickening equipment, has simplified operation, has saved a large amount of steam, has reduced energy consumption, has shortened the time.
Elutriant concentration method of the present invention adopts membrane separation technique to concentrate, and has shortened concentration time greatly, reduced energy consumption, saved many equipment, adopted membrane separation technique to concentrate simultaneously, avoided conventional when concentrated puerarin be heated for a long time and influence the drawback of quality.
Chromatographic separating process of the present invention, the sorbent material that is adopted can be polymeric adsorbents such as polymeric amide, macroporous resin, dextrane gel.
Chromatographic separating process of the present invention, the elutriant that is adopted can be pure water or the pure water that contains alcohol.
The routine operation that the present invention relates to is a known technology.
Compared with the prior art the method for preparing puerarin of the present invention has following distinguishing feature and positively effect: extracting solution of the present invention is directly gone up the chromatography column separation method without concentrating, and the time shortens 60%, and energy consumption saves 80%.Elutriant concentration method of the present invention, the equipment input saves 70%, and running cost has only 20% of ordinary method.
Owing to adopted the direct upper prop of extracting solution to separate and with the method for membrane separation technique concentrate eluant, make the present invention prepare the method for puerarin, it is simple to have technological process, easy to operate, with short production cycle, energy consumption is low, the equipment less investment, comprehensive low cost of manufacture, characteristics such as constant product quality, its remarkable benefit is beneficial to suitability for industrialized production and uses.The inventive method may be used in the extraction separation of other Chinese medicine, and the especially application of membrane separation technique has positive effect to promoting the modernization of Chinese medicine.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
The method for preparing puerarin of the present invention is carried out according to the following steps:
(1) extracts
Treated root of kudzu vine 400Kg is added in the extractor, add the water of 4-5 times of medicinal material weight, heating keeps little boiling 4 hours, and blowing repeats 3 times, merges whole extracting solutions.
(2) column chromatography
Separate going up chromatography column behind the extracting liquid filtering, detect the back with thin layer and collect the elutriant that contains puerarin.
(3) elutriant concentrates
The elutriant of collecting is passed through membrane concentration equipment, obtain containing the concentrated solution of puerarin 4-8%.
(4) crystallization, refining
Concentrated solution low temperature is placed, is had crystallization to separate out, filter crystallization, again through refining pure product of puerarin that obtain repeatedly.

Claims (4)

1, a kind of method for preparing puerarin, it is characterized in that adopting water is that solvent extracts, extracting solution is without concentrating, filter the back and directly go up the chromatography column separation, with pure water or contain alcohol the pure water wash-out, to detect the elutriant of collecting the back that contains puerarin with thin layer and adopt membrane separation technique to concentrate, concentrated solution through crystallization, refining, obtains the pure product of puerarin more at last.
2, the method for preparing puerarin according to claim 1 is characterized in that the sorbent material that chromatography is used is polymeric amide or macroporous resin or dextrane gel resin.
3, the method for preparing puerarin according to claim 1, the concentration that it is characterized in that puerarin in the concentrated solution is 4-8%.
4, the method for preparing puerarin according to claim 3 is characterized in that concentrated solution low temperature is placed, and separates out crystallization, filter crystallization, again through the refining pure product of puerarin that obtain.
CNB2004100356243A 2004-08-29 2004-08-29 Puerarin preparing process Expired - Fee Related CN1312168C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNB2004100356243A CN1312168C (en) 2004-08-29 2004-08-29 Puerarin preparing process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2004100356243A CN1312168C (en) 2004-08-29 2004-08-29 Puerarin preparing process

Publications (2)

Publication Number Publication Date
CN1629174A CN1629174A (en) 2005-06-22
CN1312168C true CN1312168C (en) 2007-04-25

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CNB2004100356243A Expired - Fee Related CN1312168C (en) 2004-08-29 2004-08-29 Puerarin preparing process

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Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100355766C (en) * 2005-10-21 2007-12-19 安徽农业大学 Method for preparing and purifying uperarin through membrane technology
CN102532111B (en) * 2010-12-24 2016-04-27 天津中新药业集团股份有限公司第六中药厂 A kind of method extracting puerarin from Chinese medicine elegant jessamine
CN103131578B (en) * 2011-11-29 2016-04-27 中润益源(北京)生物科技有限公司 A kind of scheme of root of kudzu vine comprehensive processing and utilization
CN103789366A (en) * 2014-02-21 2014-05-14 王健鹏 Method for extracting puerarin through compound enzyme hydrolysis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1129700A (en) * 1995-02-23 1996-08-28 赵爱平 Extraction and application of Lobed Kudzuvine
CN1364760A (en) * 2002-01-28 2002-08-21 杨登科 New process for extracting high purity puerarin by pure water method
CN1393248A (en) * 2001-07-03 2003-01-29 买凯 Extracting process and application of active component of pueraria root
CN1483734A (en) * 2003-07-26 2004-03-24 卓宇哲 Novel process for extracting puerarin from pueraria mirifica

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1129700A (en) * 1995-02-23 1996-08-28 赵爱平 Extraction and application of Lobed Kudzuvine
CN1393248A (en) * 2001-07-03 2003-01-29 买凯 Extracting process and application of active component of pueraria root
CN1364760A (en) * 2002-01-28 2002-08-21 杨登科 New process for extracting high purity puerarin by pure water method
CN1483734A (en) * 2003-07-26 2004-03-24 卓宇哲 Novel process for extracting puerarin from pueraria mirifica

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
优选葛根提取工艺的实验研究 王翊臻等人,医学文选,第23卷第1期 2004 *

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