CN1623557A - Application of gold ear mycelium polysaccharide for lowering blood fat and blood sugar - Google Patents
Application of gold ear mycelium polysaccharide for lowering blood fat and blood sugar Download PDFInfo
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- CN1623557A CN1623557A CN 200310106533 CN200310106533A CN1623557A CN 1623557 A CN1623557 A CN 1623557A CN 200310106533 CN200310106533 CN 200310106533 CN 200310106533 A CN200310106533 A CN 200310106533A CN 1623557 A CN1623557 A CN 1623557A
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Abstract
An application of the Golden mushroom's mycelial polyose in preparing the medicines for decreasing blood fat and blood sugar with high effect is disclosed.
Description
Technical field
The present invention relates to a kind of new purposes, especially Polysaccharides from Temelle aurantiabla mycelium TMP application aspect blood sugar lowering and blood fat reducing of Polysaccharides from Temelle aurantiabla mycelium TMP.
Background technology
Diabetes, hyperlipidemia are the commonly encountered diseases and the frequently-occurring diseases of present middle-aged and elderly people, bring out the life and health that disease seriously jeopardizes people by what diabetes and hyperlipidemia caused.Existing Western medicine class blood sugar lowering blood lipid-lowering medicine has side effect unavoidably, and the then general drug effect of Chinese medicine class blood sugar lowering blood lipid-lowering medicine is obvious inadequately.
Tremella aurantialba Bandoni et Zang is a food medicine fungus famous in Basidiomycetes, the Tremellaceae, mainly originate in the remote mountains on ground such as Yunnan Province of China, Guizhou, though its medical value early be familiar with by people, all the time based on Traditional Chinese medical theory.As putting down in writing according to Compendium of Material Medica: Tremella aurantialba Bandoni et Zang can be controlled " hypochondrium fluid retention is gathered, the stomachache incised wound ".Its flavor efficacy is " the warming middle-JIAO band is cold, and sweet in the mouth can reduce phlegm, and ends expectorant, Dingchuan, regulating QI, suppressing the hyperactive liver sun ".
In recent years, the present patent application people has finished the work of extracting the Tremella aurantialba Bandoni et Zang fruitbody polysaccharide and extract Polysaccharides from Temelle aurantiabla mycelium TMP from fermentation mycelium by groping repeatedly and constantly experiment from the Tremella aurantialba Bandoni et Zang sporophore, and the patent of invention of having applied for relevant extracting method.In the process of further further investigation, the present patent application people has had considerable progress to the understanding of golden fungus polysaccharides pharmacological action, find that it is to reducing liver cholesterol, enhancing immunity, promote hemopoietic function, all there is certain curative effect anti-ageing many aspects of waiting for a long time, especially by experiment confirm, the Polysaccharides from Temelle aurantiabla mycelium TMP that from fermentation mycelium, extracts (Polysaccharides from Temelle aurantiabla mycelium, TMP) has significant blood sugar reducing function (though the Tremella aurantialba Bandoni et Zang fruitbody polysaccharide also has similar effect, but it yields poorly, extract difficulty, therefore do not have commercial value, be difficult to practical application).Hyperglycemia is the common pathological phenomenon of carbohydrate metabolism disturbance unusually with hyperlipemia and lipid metabolism.For this reason, when research TMP is to the hyperglycemia metabolic effect,, further observe TMP to lipometabolic effect by detecting plasma triglyceride and cholesterol levels.
Summary of the invention
In view of above reason, the objective of the invention is to: provide the application of Polysaccharides from Temelle aurantiabla mycelium TMP blood sugar lowering and blood fat reducing, thereby open vast vistas in the business development utilization of pharmaceutical field for Tremella aurantialba Bandoni et Zang, " Gu Lian " that makes motherland's medical science leaving the new flower in epoch now.
The present patent application people discovers and through experiment confirm repeatedly through rigorous, obtain the Polysaccharides from Temelle aurantiabla mycelium TMP that extracts the mycelium through the lumbar injection approach or oral route gives hyperglycemia and/or hyperlipemia rat can significantly reduce its blood glucose and/or blood fat from fermentation institute, confirm that therefore Polysaccharides from Temelle aurantiabla mycelium TMP has as the purposes for preparing blood sugar lowering and hypolipidemic medicine.Especially, because Polysaccharides from Temelle aurantiabla mycelium TMP is a kind of pure Chinese medicinal preparation, and original " regulating QI, suppressing the hyperactive liver sun " effect of etc.ing itself, therefore compare with existing hypoglycemic medicine, not only instant effect, effect are remarkable, and take afterwards useful and harmless.
Description of drawings
Fig. 1 is that the blood glucose value of measuring administration 4,7,14,23d respectively changes sketch map
The specific embodiment
In conjunction with the embodiments method of the present invention is described further down.
Embodiment 1
The present embodiment material therefor is as follows:
1, Polysaccharides from Temelle aurantiabla mycelium TMP: prepare by laboratory ferment cultivation and extraction by the attached steps A-G in back.
2, animal: male SD rat, body weight 190-210g (the laboratory animal quality certification number: Shanghai is moving closes the card word No. 152).
3, medicine: homemade analytical reagent such as alloxan, JIANGZHILING PIAN, glucobay (acarbose), anthrone, concentrated sulphuric acid, potassium hydroxide, sodium chloride, n-butyl alcohol, chloroform.
4, test kit: the glucose enzyme process is measured test kit, enzyme process serum (slurry) triglyceride test kit, serum (slurry) enzyme process T-CHOL mensuration test kit.
5, instrument: 722 type spectrophotometers, vortex mixed instrument, high speed tabletop centrifuge, high pressure steam sterilizer of portable type, homogenizer, high speed refrigerated centrifuge, thermostat water bath, rotary shaking table, 10 liters of fermentation tanks.
Above Polysaccharides from Temelle aurantiabla mycelium TMP fermentation culture and extraction preparation process are:
A. put jar---cultured fermentation tank is put into centrifuge, and mycelium is taken out in centrifugalize, washes the back with water and dries;
B, steaming and decocting---in mycelium, add times pure water, boil, dry with centrifuge;
C. hydrolysis---cooking liquor is merged, add the NaOH hydrolysis;
D. centrifugal---separate hydrolyzed solution, get super liquid;
E. ultrafiltration---with 0.1 micron membrane microfiltration, the membrane ultrafiltration of reuse molecular weight 5000;
F, concentrated---with ethanol ultrafiltration concentration liquid, make it precipitation;
G, crystallization---leach the concentrating and precipitating thing,, obtain Polysaccharides from Temelle aurantiabla mycelium TMP with neutral spirits washing back oven dry;
The experimental technique of present embodiment is: choose the fasting plasma glucose level the normal male SD of 3.5mmol/L--6.3mmol/L scope rat, 4%alloxan injection with citrate buffer solution (pH4.6) sterilization back preparation, by body weight 140mg/kg dosage disposable celiac injection moulding, behind the 72h, selecting fasting blood sugar to compile at random greater than the rat of 10mmol/L is 6 groups, every group 8, blood glucose value difference is less than 1.11mmol/L between group.If positive control (I group) is irritated stomach (ig.) and is given normal saline; The positive drug contrast is respectively insoral treatment (II group), ig100mg/kg.d dosage insoral; Acarbose treatment (III group), ig.20mg/kg.d dosage acarbose; TMP treats basic, normal, high three kinds of dosage (be respectively IV, V, VI group), respectively with 50,100,200mg/kg.d dosage ig TMP.
Observation administration 4d, 7d, each organizes rat plasma glucose and blood lactic acid content.Continue to observe blood glucose value, hepatic glycogen content that continuous 14d, 23d give hyperglycemic rat behind 100mg/kg.d dosage TMP and the insoral.Choose the fasting plasma glucose level the normal male SD of 3.5mmol/L-6.3mmol/L scope rat, 4%alloxan injection with citrate buffer solution (pH4.6) sterilization back preparation, by body weight 140mg/kg dosage disposable celiac injection moulding, behind the 72h, selecting fasting blood sugar to compile at random greater than the rat of 10mmol/L is 6 groups, every group 8, blood glucose value difference is less than 1.11mmol/L between group.If positive control (I group) is irritated stomach (ig.) and is given normal saline; The positive drug contrast is respectively insoral treatment (II group), ig100mg/kg.d dosage insoral; Acarbose treatment (III group), ig.20mg/kg.d dosage acarbose; TMP treats basic, normal, high three kinds of dosage (be respectively IV, V, VI group), respectively with 50,100,200mg/kg.d dosage ig TMP.
Observation administration 4d, 7d, each organizes rat plasma glucose, triglyceride, cholesterol and blood lactic acid content.Continue to observe blood glucose value, hepatic glycogen content that continuous 14d, 23d give hyperglycemic rat behind 100mg/kg.d dosage TMP and the insoral.
Tried the rat plasma glucose content before and after the mensuration administration and changed, be the results are shown in Table 1:
By table 1 as seen, TMP administration 4d, 7d, compared with the control, treatment group hyperglycemic rat blood glucose all significantly reduces (p＜0.05).After one week of administration, each is organized blood sugar level and still significantly is lower than matched group (p＜0.05), and as seen, ig.TMP4d can effectively reduce the blood glucose of hyperglycemic rat, and one week of administration still can be kept effect.
Table 1 TMP induces the influence of hyperglycemic rat plasma glucose levels to alloxan
Group number of rats dosage plasma glucose content (mmol/L)
Group Number Dose
plasma?glucose?content
(mg/kg) 0d 4d 7d
I 8 normal saline 22.34 ± 6.24 23.71 ± 6.11 27.05 ± 3.84
II 8 100 22.58±5.63 13.12±6.89
* 18.47±8.29
*
III 8 20 22.79±5.60 15.80±5.21
* 20.28±3.09
**
IV 8 50 22.89±5.10 13.07±6.85
* 17.59±7.95
*
V 8 100 23.10±5.73 11.71±6.22
** 15.48±8.45
**
VI 8 200 22.68±5.29 15.49±7.03
* 17.88±6.89
*
t?I-II 2.515 2.347
t?I-III 2.678 3.723
t?I-IV 2.372 2.499
t?I-V 3.699 3.263
t?I-VI 2.424 2.324
df＝13?t
0.05＝2.160?t
0.01＝3.012；df＝14?t
0.01＝2.145?t
0.01＝2.977
Annotate: molding Mus II, III, IV, VI after grouping organize each dead one of rat, and the result tests by the actual number of rats of participating in the experiment.
Continuously ig.100mg/kg.d TMP, insoral 23d measure the blood glucose value of administration 4,7,14,23d respectively, the results are shown in Figure 1:
As shown in the figure, treat 14d continuously, the blood sugar content of insoral and TMP treatment group is respectively: 13.06 ± 7.57mmol/L, 12.68 ± 7.69mmol/L; Through the t check analysis, 24.60 ± 4.58mmol/L compares with the matched group blood sugar level, and the blood glucose of two groups of rats reduces equal significance (p＜0.05).The blood glucose value that 23d is three groups is respectively: 25.12 ± 7.09 (n=7), 12.38 ± 6.93 (n=5), 12.96 ± 6.37 (n=7), two treatments organize compared with the control that blood glucose, blood fat reduce equal significance (p＜0.05).
Embodiment 2
Present embodiment uses and embodiment 1 identical materials, the Polysaccharides from Temelle aurantiabla mycelium TMP fermentation culture is also identical with embodiment 1 with the extraction preparation process, and what difference was present embodiment mensuration is the influence that TMP causes hyperglycemic rat plasma cholesterol and triglyceride levels before and after the administration to alloxan
TMP causes the lipometabolic influence of hyperglycemic rat to alloxan, sees Table 2
Table 2:TMP causes hyperglycemic rat blood plasma to alloxan
The influence of cholesterol and triglyceride levels
Group number of rats dosage plasma cholesterol plasma triglyceride
(mg/kg) (mmol/L) (mmol/L)
Group Number Dose cholesterin triglyceride
I 8 normal saline 2.079 ± 0.528 0.961 ± 0.565
II 8 100 1.984±0.47 0.456±0.108
*
III 8 20 1.718±0.451 0.509±0.264
IV 8 50 1.886±0.299 0.621±0.144
V 8 100 1.691±0.449 0.498±0.099
*
VI 8 200 2.195±0.387 0.788±0.132
t?I-II 0.380 2.483
t?I-III 1.471 2.049
t?I-IV 0.938 1.649
t?I-V 1.583 2.283
t?I-VI 0.510 0.843
df＝14，t
0.05＝2.146，t
0.01＝2.977
By table 2 as seen, after one week of administration, TMP is to the influence of the inductive hyperglycemic rat plasma cholesterol of alloxan content not significantly (p＞0.05), but the plasma triglyceride level of hyperglycemic rat significantly descended (p＜0.05).By the test overall process as seen, TMP treatment group blood glucose, blood fat fluctuating margin are less, show its not only can be at short notice effective reducing blood sugar and blood fat, and the reducing blood sugar and blood fat effect of long term administration is also stable and significantly.
Embodiment 3
Adopt following method to produce Polysaccharides from Temelle aurantiabla mycelium TMP:
A. put jar---cultured fermentation tank is put into centrifuge, and mycelium is taken out in centrifugalize, washes the back with water and dries;
B, steaming and decocting---in mycelium, add times pure water, boil, dry with centrifuge;
C. hydrolysis---cooking liquor is merged, add the KOH hydrolysis;
D. centrifugal---separate hydrolyzed solution, get super liquid;
E. ultrafiltration---with 0.1 micron membrane microfiltration, the membrane ultrafiltration of reuse molecular weight 5000;
F, concentrated---with ethanol ultrafiltration concentration liquid, make it precipitation;
G, crystallization---leach the concentrating and precipitating thing,, obtain Polysaccharides from Temelle aurantiabla mycelium TMP with neutral spirits washing back oven dry;
Make aqueous solution then, heating for dissolving behind the mix homogeneously, is distributed into injection that 10/ml/ props up concentration and packs into and seal in the medicine bottle, and product is made in sterilization.
Embodiment four
Take embodiment three same methods to produce Polysaccharides from Temelle aurantiabla mycelium TMP, make tablet by known tabletting technology and equipment.
In a word, the TMP of lumbar injection 50mg/kg dosage can make the blood pressure and blood lipoid of the inductive hyperglycemic rat of alloxan significantly reduce in 7 days, and not cause the increase of insulin secretion.All its blood pressure and blood lipoid level can be effectively reduced with the route of administration of irritating stomach to TMP, the blood glucose and the stable decline of blood lipid level of hyperglycemic rat can be made in 3 weeks of successive administration with hyperglycemic rat 50,100,200mg/kg dosage.TMP also can reduce the plasma triglyceride level level of the inductive hyperglycemic rat of alloxan simultaneously.Can confirm thus: Polysaccharides from Temelle aurantiabla mycelium TMP, be that TMP fully can be as the ideal medicament of preparation blood sugar lowering and blood fat reducing, this medicine both can be an injection, also can be oral liquid.
Can show that with the efficacy trial of existing reducing blood sugar and blood fat medicine the Polysaccharides from Temelle aurantiabla mycelium TMP drug effect is fast, the reducing blood sugar and blood fat effect is obvious.Because itself be a kind of pure Chinese medicinal preparation, and original effects such as " regulating QI, suppressing the hyperactive liver sun ", so Polysaccharides from Temelle aurantiabla mycelium TMP is taken back useful and harmless.The present invention has not only opened a brand-new approach for deep development utilizes Tremella aurantialba Bandoni et Zang, and for the blood sugar lowering blood fat reducing provides new desirable Chinese medicine, will bring good economic benefit and social benefit.
Claims (6)
1. the application of Polysaccharides from Temelle aurantiabla mycelium TMP in the medicine of preparation blood sugar lowering.
2, the application of Polysaccharides from Temelle aurantiabla mycelium TMP in the medicine of preparation blood fat reducing.
3, according to the purposes of claim 1 or 2 described Polysaccharides from Temelle aurantiabla mycelium TMP, it is characterized in that: Polysaccharides from Temelle aurantiabla mycelium TMP is prepared into said dosage form on any pharmaceutics.
4. the purposes of Polysaccharides from Temelle aurantiabla mycelium TMP according to claim 3 is characterized in that: with the injectable drug of Polysaccharides from Temelle aurantiabla mycelium TMP as preparation blood sugar lowering or blood fat reducing.
5, the purposes of Polysaccharides from Temelle aurantiabla mycelium TMP according to claim 3 is characterized in that: with the oral drugs of Polysaccharides from Temelle aurantiabla mycelium TMP as preparation blood sugar lowering or blood fat reducing.
6. according to the purposes of claim 1 or 2 described Polysaccharides from Temelle aurantiabla mycelium TMP, it is characterized in that: described Polysaccharides from Temelle aurantiabla mycelium TMP extracts according to the following steps:
A. put jar---cultured fermentation tank is put into centrifuge, and mycelium is taken out in centrifugalize, washes the back with water and dries;
B, steaming and decocting---in mycelium, add times pure water, boil, dry with centrifuge;
C. hydrolysis---cooking liquor is merged, add the NaOH hydrolysis;
D. centrifugal---separate hydrolyzed solution, get super liquid;
E. ultrafiltration---with 0.1 micron membrane microfiltration, the membrane ultrafiltration of reuse molecular weight 5000;
F, concentrated---with ethanol ultrafiltration concentration liquid, make it precipitation;
G, crystallization---leach the concentrating and precipitating thing,, obtain Polysaccharides from Temelle aurantiabla mycelium TMP with neutral spirits washing back oven dry.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310106533 CN1623557A (en) | 2003-12-05 | 2003-12-05 | Application of gold ear mycelium polysaccharide for lowering blood fat and blood sugar |
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| CN 200310106533 CN1623557A (en) | 2003-12-05 | 2003-12-05 | Application of gold ear mycelium polysaccharide for lowering blood fat and blood sugar |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225361B (en) * | 2007-01-18 | 2010-07-07 | 章克昌 | Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid |
| CN104382000A (en) * | 2014-12-04 | 2015-03-04 | 李振铭 | Coriolus versicolor, tremella aurantialba and poria cocos polysaccharide and preparation process thereof |
| CN107212333A (en) * | 2017-07-10 | 2017-09-29 | 张志才 | The fermentate of the golden solid state transformed Chinese yam of ear and its application |
| CN115746158A (en) * | 2022-11-29 | 2023-03-07 | 中国药科大学 | Tremella aurantialba polysaccharide and preparation method and application thereof |
-
2003
- 2003-12-05 CN CN 200310106533 patent/CN1623557A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225361B (en) * | 2007-01-18 | 2010-07-07 | 章克昌 | Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid |
| CN104382000A (en) * | 2014-12-04 | 2015-03-04 | 李振铭 | Coriolus versicolor, tremella aurantialba and poria cocos polysaccharide and preparation process thereof |
| CN107212333A (en) * | 2017-07-10 | 2017-09-29 | 张志才 | The fermentate of the golden solid state transformed Chinese yam of ear and its application |
| CN115746158A (en) * | 2022-11-29 | 2023-03-07 | 中国药科大学 | Tremella aurantialba polysaccharide and preparation method and application thereof |
| CN115746158B (en) * | 2022-11-29 | 2024-04-26 | 中国药科大学 | Tremella aurantialba polysaccharide and preparation method and application thereof |
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