CN1611938A - Pyloric spirillum rapid diagnosis reagent - Google Patents
Pyloric spirillum rapid diagnosis reagent Download PDFInfo
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- CN1611938A CN1611938A CN 200310108204 CN200310108204A CN1611938A CN 1611938 A CN1611938 A CN 1611938A CN 200310108204 CN200310108204 CN 200310108204 CN 200310108204 A CN200310108204 A CN 200310108204A CN 1611938 A CN1611938 A CN 1611938A
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- reagent
- diagnosis reagent
- helicobacter pylori
- urea
- phenol red
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Abstract
This invention is a kind of quickly diagnose reagent of pylorus screw bacterium. It is a kind of aqueous solution or PBS solution. Its components are urea 0.010-0.030g/ml, phenol red 0.45-0.65mg/ml, sodium chloride 2.5-5.0mg/ml, disodium hydrogen phosphate 1.5-2.5mg/ml. The preparation of the reagent used in the invention is easy, the operation is also easy and the precision is high, color-developing is quickly. It is non-poisonous, tastefree and the cost is low. It is a complement method of 'golden standard', a method of endoscope examination and mucous membrane tissue pathological examination. It is a reagent that has the worthy of generalization.
Description
Technical field
The present invention relates to a kind of diagnostic reagent, relate in particular to a kind of helicobacter pylori diagnostic reagent.
Background technology
Helicobacter pylori (Helicobacter pylori, be called for short Hp, down together) higher to crowd's infection rate, chronic gastritis that causes and peptic ulcer diagnosed Hp to infect mucosa tissue pathological examination commonly used, the cultivation of mucosa tissue Hp bacterium and employing to people's the healthy significant damage that caused in the past
13C or
14The C-urea breath test.Outside the test condition that histopathologic examination and microbe growth are had relatively high expectations, still to accept gastrocopy.
13C or
14The C-urea breath test has more sensitivity and the high characteristics of specificity, but costs an arm and a leg.Application enzyme linked immunosorbent assay (ELISA) detects the anti-Hp antibody of patients serum and have clear superiority aspect seroepidemiological survey, needs just drop to normally after 5～June but Hp eradicates the back antibody titer, can not examine the elimination curative effect.
It is a kind of 95108004.0 that Chinese patent application number discloses, and discloses a kind of helicobacter pylori fast diagnosis reagent, though can overcome the defective of above-mentioned detection method, but, need a kind of diluted plasma reaction unit of preparation, the precision of test has been subjected to certain influence, and cost is higher relatively.
The technical issues that need to address of the present invention are to disclose a kind of helicobacter pylori fast diagnosis reagent, to overcome the above-mentioned defective that prior art exists.
Technical scheme of the present invention:
A kind of helicobacter pylori fast diagnosis reagent is a kind of aqueous solution or PBS (phosphate buffer) solution, and its component and content comprise:
Urea 0.010～0.030g/ml
Phenol red 0.45～0.65mg/ml
Sodium chloride 2.5～5.0mg/ml
Sodium hydrogen phosphate 1.5～2.5mg/ml
Preferred ingredients and content comprise:
Urea 0.015～0.025g/ml, phenol red 0.50～0.60mg/ml, sodium chloride 3～4mg/ml, sodium hydrogen phosphate 1.8～2.2mg/ml, agar 0.01～0.015mg/ml.
Wherein the effect of urea is to decompose ammonification under the effect of bacteriogenic urease;
Phenol red effect is an indicator, takes on a red color in the solution of alkalescence;
The effect of sodium chloride and sodium hydrogen phosphate is solution damping fluid (phosphate buffer);
The effect of agar is with solution aggegation agglutination thing;
Helicobacter pylori fast diagnosis reagent of the present invention is preparation like this:
With phenol red aqueous solution or the PBS (phosphate buffer) of being dissolved in, add other component then, and be 4.8～5.8 with the pH value of hydrochloric acid conditioning solution, be helicobacter pylori fast diagnosis reagent of the present invention.
Helicobacter pylori fast diagnosis reagent of the present invention is to use like this:
1. place agents useful for same under the room temperature half an hour before using.Check the reagent color, as be orange, then carefully use it.
2. get the curved greatly side gastric mucosa sample of prepyloric region (in pylorus 5cm).Tear gummed paper and expose agaropectin, biopsy specimen is inserted gel, be stained with gummed paper again with fine-pointed forceps or toothpick.
3. write down patient name, numbering and sample and put into the time.
4. agent plate is placed under the room temperature (15-35 ℃), reagent is by the red then test ending of xanthochromia in the time of 1-3 minute.Observe again when then sample being kept 30 minutes as nondiscolouring.
Helicobacter pylori fast diagnosis reagent of the present invention is easy to prepare, operation is very easy during use, and precision height, colour developing is fast, nonpoisonous and tasteless, cost is low, for a kind of easy to use and a kind of and disease of upper digestive tract diagnosis " goldstandard "-----endoscopy and the supporting inspection method of mucous membrane tissue pathologic finding, is the helicobacter pylori fast diagnosis reagent with promotional value.
Embodiment
Embodiment 1
0.55mg is phenol red levigate, be dissolved in the 1ml water, add 0.02g urea then, 4mg sodium chloride, 2mg sodium hydrogen phosphate/ml, the 0.01mg agar powder to pH=4.8, promptly obtains helicobacter pylori fast diagnosis reagent of the present invention with hydrochloric acid conditioning solution.
Embodiment 2
0.50mg is phenol red levigate, be dissolved among the 1ml PBS (phosphate buffer), add 0.022g urea then, 4mg sodium chloride, 2mg sodium hydrogen phosphate/ml, 0.01mg agar powder, to pH=5.8, promptly obtain helicobacter pylori fast diagnosis reagent of the present invention with hydrochloric acid conditioning solution.
Embodiment 3
Get the helicobacter pylori fast diagnosis reagent of embodiment 1, operation steps:
5. use preceding half an hour of agents useful for same places under the room temperature.Check the reagent color, as be orange, then carefully use it.
6. get the curved greatly side stomach lining sample of prepyloric region (in pylorus 5cm).Tear gummed paper and expose agaropectin, biopsy specimen is inserted gel, be stained with gummed paper again with fine-pointed forceps or toothpick.
7. write down patient name, numbering and sample and put into the time.
8. agent plate is placed under the room temperature (15-35 degree), in the time of 1-3 minute reagent red by xanthochromia be the helicobacter pylori positive, then test ending.Observe again when then sample being remained to 30 minutes as nondiscolouring.
Claims (2)
1. a helicobacter pylori fast diagnosis reagent is characterized in that, is a kind of aqueous solution or PBS (phosphate buffer), and its component and content comprise:
Urea 0.010～0.030g/ml
Phenol red 0.45～0.65mg/ml
Sodium chloride 2.5～5.0mg/ml
Sodium hydrogen phosphate 1.5～2.5mg/ml.
2. helicobacter pylori fast diagnosis reagent according to claim 1 is characterized in that, component and content comprise:
Urea 0.015～0.025g/ml, phenol red 0.50～0.60mg/ml, sodium chloride 3～4mg/ml, sodium hydrogen phosphate 1.8～2.2mg/ml, agar 0.01～0.015mg/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101082049A CN100392404C (en) | 2003-10-27 | 2003-10-27 | Pyloric spirillum rapid diagnosis reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101082049A CN100392404C (en) | 2003-10-27 | 2003-10-27 | Pyloric spirillum rapid diagnosis reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1611938A true CN1611938A (en) | 2005-05-04 |
| CN100392404C CN100392404C (en) | 2008-06-04 |
Family
ID=34758513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101082049A Expired - Fee Related CN100392404C (en) | 2003-10-27 | 2003-10-27 | Pyloric spirillum rapid diagnosis reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100392404C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
| CN104458725A (en) * | 2014-12-08 | 2015-03-25 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN105755105A (en) * | 2016-04-19 | 2016-07-13 | 夏少时 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
| CN113092459A (en) * | 2021-04-09 | 2021-07-09 | 许奕鹏 | Gel reagent and kit for fast screening of urease activity and fast screening method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5839255B2 (en) * | 1975-11-21 | 1983-08-29 | 日本精工株式会社 | Jishiyakuoshiyoshitakuratsuchireri - Zujikuuukesouchino Jidouchiyoshinsouchi |
| CN1040228C (en) * | 1990-12-11 | 1998-10-14 | 湖南省安乡县人民医院 | Technology to produce medicinal box for quick diagnosis of pylorus vibrio |
-
2003
- 2003-10-27 CN CNB2003101082049A patent/CN100392404C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
| CN104458725A (en) * | 2014-12-08 | 2015-03-25 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN104458725B (en) * | 2014-12-08 | 2017-05-10 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN105755105A (en) * | 2016-04-19 | 2016-07-13 | 夏少时 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
| CN113092459A (en) * | 2021-04-09 | 2021-07-09 | 许奕鹏 | Gel reagent and kit for fast screening of urease activity and fast screening method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100392404C (en) | 2008-06-04 |
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Granted publication date: 20080604 Termination date: 20131027 |