CN105755105A - Reagent and kit for detecting helicobacter pylori and preparation method of kit - Google Patents
Reagent and kit for detecting helicobacter pylori and preparation method of kit Download PDFInfo
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- CN105755105A CN105755105A CN201610242501.XA CN201610242501A CN105755105A CN 105755105 A CN105755105 A CN 105755105A CN 201610242501 A CN201610242501 A CN 201610242501A CN 105755105 A CN105755105 A CN 105755105A
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- helicobacter pylori
- test kit
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- kit
- carbamide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Abstract
The invention belongs to the technical field of biology and particularly relates to a reagent and a kit for detecting helicobacter pylori and a preparation method of the kit.The kit comprises urea and phenol sulfonphthalein in a mass ratio of 1000:1.0-1.5.The preparation method of the kit includes regulating a pH value of water at 6.5-6.9 by glacial acetic acid, infiltrating degreasing cotton to be neutral, dewatering and drying for standby use; winding the treated degreasing cotton around one end of bamboo sticks, and sterilizing to obtain cotton swabs; grinding the urea and the phenol sulfonphthalein with uniformly mixing, adding water to dissolve into a pasty solution, filling every plastic bucket with 0.15-0.25 mL of the solution, drying and covering to seal; subjecting the cotton swabs, control test strips and the plastic buckets to vacuum sealing of plastic bags and sterilization so as to obtain the kit.The reagent for detecting the helicobacter pylori is simple and low in cost, and the kit for detecting the helicobacter pylori cannot cause any harm, pain and toxic and side effects to human bodies, is simple to operate and quick in detection and can be widely applied.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of detect the reagent of helicobacter pylori, test kit and preparation method thereof.
Background technology
Stomach Helicobacter pylori Helicobacter Pylori(is called for short HP) it is a kind of helical form gram negative bacteria in human stomach mucosa.This antibacterial is to cause chronic gastritis, gastric ulcer and the duodenal ulcer even arch-criminal of gastric cancer.It is generally acknowledged that the clinical course that stomach Helicobacter pylori infects is such that settling down infection after stomach Helicobacter pylori per os arrives gastric mucosa, chronic, superficial gastritis is caused through several weeks or several months, develop into duodenal ulcer, gastric ulcer, lymphoproliferative gastric lymphoma, chronic atrophic gastritis etc. after several years or many decades, and the latter is the factor causing gastric cancer the most dangerous.Brainstrust is thought, stomach Helicobacter pylori infects and makes the danger got a cancer of the stomach add
2.7-12 times, without Helicobacter pylori infection, at least the gastric cancer of 35%-89% will not occur.
Since within 1983, taking the success of biopsy specimen separation and Culture by gastroscope, the diagnosis to helicobacter pylori infections has had been developed in many methods, includes bacteriology, pathology, serology, isotopic tracing, molecular biology etc..But total says, in terms of collection of specimens angle, aggressive and the big class of Non-Invasive two can be divided into.Invasive method refers mainly to must pass through gastroscope and takes the method that biopsy specimen checks, is the conventional method of current disease for digest subject, and it includes separation and Culture and direct smear, rapid urease test, the drug sensitive test of antibacterial.Noninvasive method refers mainly to not take, by gastroscope, the method that biopsy specimen diagnosing helicobacter pylori specimen infects.This kind of method includes antibody test, Detection of antigen, urease 13C/14C breath test etc..
Chinese patent CN 102688505A discloses a kind of compositions detecting helicobacter pylori gastrin IR cells and reagent, said composition and reagent under gastroscope it is observed that helicobacter pylori is in the distribution situation of gastric.But, this patented method is carried out in vivo, and experimental implementation is required height, and with certain blindness when taking biopsy.
Chinese patent CN103293305A discloses a kind of oral cavity helicobacter infection detection method and the saliva test panel for detection, take saliva to be placed in test glass, and drip buffer and fully mix, draw mixed liquor and instill the sample-adding window of sputum test plate, 5-15 minute observed result.The sputum test plate used in detection method has sputum test bar, sputum test bar has glass layer, saliva absorption ply of paper, gold colloidal ply of paper, glass layer, absorption ply of paper join end to end successively and are pasted together, glass layer is sequentially provided with control line, p-wire along absorption ply of paper to gold colloidal ply of paper direction, wherein with sheep polyclonal antibody on control line, with urease antibody or flagellar antibody on p-wire, with urease antibody gold colloidal or flagellar antibody gold colloidal on gold colloidal ply of paper.Urease antibody or flagellar antibody are the antibody prepared by urease or the flagellar antigen of the release of poisonous type pylori respectively.But, this patented method raw material is complicated, and cost is high.
Summary of the invention
For overcoming drawbacks described above, it is an object of the invention to provide and a kind of detect the reagent of helicobacter pylori, test kit and preparation method thereof.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of reagent detecting helicobacter pylori, including carbamide and phenol red.
Preferably, described carbamide and phenol red mass ratio are 1000:1.0-1.5.
Preferably, described carbamide and phenol red mass ratio are 1000:1.2.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
Preferably, described control stripes bar long 12-16 mm, wide 3-6mm, point yellow, redness, rose band, the longest 2-4mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.5-6.9, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) absorbent cotton after bamboo let one ends wound step (1) processes, sterilization, prepare cotton swab；
(3) by carbamide and phenol red pulverizing, mix homogeneously, being dissolved in water into pasty solutions, described carbamide, phenol red and water mass ratio are 1000:1.0-1.5:250-350, each plastics barreled 0.15-0.25 mL solution, are dried, seal；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Preferably, step (2) described bamboo let specification is diameter 1.5-2 mm, long 40-50mm, a diameter of 2.5-3.5 mm of the absorbent cotton of winding, long 5-6 mm.
Preferably, step (3) described Plastic Drum diameter 4.5-5.5 mm, a height of 13-16 mm.
The positive beneficial effect of the present invention:
1. the present invention detects the reagent of helicobacter pylori simply, with low cost.
2. to detect the test kit preparation method of helicobacter pylori simple for the present invention, operation can be realized in vitro, phenol red consumption is little, develop the color sensitive, strong positive and the positive can be distinguished very well, solve because gastroduodenal mucosa takes on a red color, and phenolic red indicator also takes on a red color when there being Helicobacter pylori infection, the problem of the phenol red poor contrast in the coloring of gastroduodenal mucosa.
3. the present invention uses the pH value of glacial acetic acid regulation water to be 6.5-6.9, can neutralize the alkalescence of absorbent cotton very well, makes the aobvious neutrality of cotton swab, can be appropriately determined in oral cavity and whether also have urease.
Test kit the most of the present invention is for the detection of helicobacter pylori, to human body without any infringement, misery, toxicity and taboo, the present invention is based on helicobacter pylori and secretes a large amount of high activity carbamide enzyme viability, containing urease in patient's saliva of buccal cavity too, urease promotes that carbamide decomposes and produces ammonia, causes pH value to raise simultaneously, make the principle of indicator discoloration to judge testing result, simple to operate, detection quickly, can extensively be applied.
Detailed description of the invention
Below in conjunction with some detailed description of the invention, the present invention is further described.
Embodiment 1
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 15mm of control stripes bar, wide 5mm, point yellow, redness, rose band, the long 3mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.5, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 1.5
Mm, the absorbent cotton after the process of long 40mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 2.5 mm of the absorbent cotton of winding, long 5 mm；
(3) being pulverized by carbamide 1000g and phenol red 1.0g, mix homogeneously in a reservoir, the 300mL that adds water is dissolved into pasty state, each plastics barreled 0.15 mL solution, is dried, seals；Described Plastic Drum diameter 5 mm, a height of 15mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 2
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.1 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 12mm of control stripes bar, wide 3mm, point yellow, redness, rose band, the long 2mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.6, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 1.6
Mm, the absorbent cotton after the process of long 45mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 2.6 mm of the absorbent cotton of winding, length 5.5
mm；
(3) being pulverized by carbamide 1000g and phenol red 1.1g, mix homogeneously in a reservoir, the 250mL that adds water is dissolved into pasty state, each plastics barreled 0.16 mL solution, is dried, seals；Described Plastic Drum diameter 4.5 mm, a height of 13mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 3
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.15 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 14mm of control stripes bar, wide 4mm, point yellow, redness, rose band, the long 3mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.7, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 1.7
Mm, the absorbent cotton after the process of long 46mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 2.8 mm of the absorbent cotton of winding, length 5.7
mm；
(3) being pulverized by carbamide 1000g and phenol red 1.15g, mix homogeneously in a reservoir, the 280mL that adds water is dissolved into pasty state, each plastics barreled 0.18 mL solution, is dried, seals；Described Plastic Drum diameter 4.6 mm, a height of 16mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 4
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.2 and phenol red.
A kind of test kit detecting helicobacter pylori, including reagent, cotton swab and control stripes bar that right is above-mentioned.
The described long 15mm of control stripes bar, wide 5mm, point yellow, redness, rose band, the long 3mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.8, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 1.5
Mm, the absorbent cotton after the process of long 50mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 3 mm of the absorbent cotton of winding, long 5 mm；
(3) being pulverized by carbamide 1000g and phenol red 1.2g, mix homogeneously in a reservoir, the 300mL that adds water is dissolved into pasty state, each plastics barreled 0.2 mL solution, is dried, seals；Described Plastic Drum diameter 5 mm, a height of 15mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 5
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.25 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 16mm of control stripes bar, wide 6mm, point yellow, redness, rose band, the long 3mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.85, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 1.8mm, the absorbent cotton after the process of long 48mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 3.1 mm of the absorbent cotton of winding, length 5.5
mm；
(3) being pulverized by carbamide 1000g and phenol red 1.25g, mix homogeneously in a reservoir, the 310mL that adds water is dissolved into pasty state, each plastics barreled 0.21 mL solution, is dried, seals；Described Plastic Drum diameter 5.1 mm, a height of 14mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 6
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.3 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 15mm of control stripes bar, wide 6mm, point yellow, redness, rose band, the long 2.5mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.9, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 2.0
Mm, the absorbent cotton after the process of long 50mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 3.2 mm, long 6.0mm of the absorbent cotton of winding；
(3) being pulverized by carbamide 1000g and phenol red 1.3g, mix homogeneously in a reservoir, the 320mL that adds water is dissolved into pasty state, each plastics barreled 0.23 mL solution, is dried, seals；Described Plastic Drum diameter 5.4 mm, a height of 15mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 7
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.4 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 15mm of control stripes bar, wide 5mm, point yellow, redness, rose band, the long 4mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.9, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 2.0
Mm, the absorbent cotton after the process of long 50mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 3.5 mm, long 6.0mm of the absorbent cotton of winding；
(3) being pulverized by carbamide 1000g and phenol red 1.4g, mix homogeneously in a reservoir, the 340mL that adds water is dissolved into pasty state, each plastics barreled 0.24 mL solution, is dried, seals；Described Plastic Drum diameter 5.2 mm, a height of 15mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Embodiment 8
A kind of reagent detecting helicobacter pylori, including the carbamide that mass ratio is 1000:1.5 and phenol red.
A kind of test kit detecting helicobacter pylori, including above-mentioned reagent, cotton swab and control stripes bar.
The described long 16mm of control stripes bar, wide 6mm, point yellow, redness, rose band, the long 4mm of each vitta.
The preparation method of a kind of test kit detecting helicobacter pylori, comprises the following steps:
(1) using glacial acetic acid regulation water pH value is 6.9, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) at diameter 2.0
Mm, the absorbent cotton after the process of long 50mm bamboo let one ends wound step (1), sterilization, prepare cotton swab, a diameter of 3.5 mm, long 5.0mm of the absorbent cotton of winding；
(3) being pulverized by carbamide 1000g and phenol red 1.5g, mix homogeneously in a reservoir, the 350mL that adds water is dissolved into pasty state, each plastics barreled 0.25 mL solution, is dried, seals；Described Plastic Drum diameter 5.5 mm, a height of 16mm；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
Clinical case
1. patient data:
Experimental group: 163 example patients, uses the detection of embodiment 4 test kit, and ambient temperature is 10-35 DEG C, and detected person is forbidden in detecting front 30-60min to drink water, gargle, and does not eat Acidity of Aikalinity food；Use gastroscope to take biopsy specimen detection method to examine simultaneously；
2. use process
The vacuum releasing test kit seals, cotton swab is put into detected person's mouth Sublingual with of absorbent cotton, is immediately placed in taking out after saliva in its absorbent cotton infiltration mouth in Plastic Drum, rotates cotton swab simultaneously, the saliva on cotton swab is made fully to concoct with indicator, after waiting 1-10 second, in observation box, color change, carries out color contrast with control stripes bar, judge helicobacter pylori yin and yang attribute, redness is positive, and rose is strong positive, invariant color or light yellow be feminine gender.
It is consistent that test kit testing result the most of the present invention takes biopsy specimen detection method testing result with gastroscope, accuracy rate 100%, illustrates that test kit of the present invention may be used for the detection of helicobacter pylori.
Claims (8)
1. the reagent detecting helicobacter pylori, it is characterised in that include carbamide and phenol red.
The reagent of detection helicobacter pylori the most according to claim 1, it is characterised in that described carbamide and phenol red mass ratio are 1000:1.0-1.5.
The reagent of detection helicobacter pylori the most according to claim 2, it is characterised in that described carbamide and phenol red mass ratio are 1000:1.2.
4. detect a test kit for helicobacter pylori, including the reagent described in claim 1, cotton swab and control stripes bar.
The test kit of detection helicobacter pylori the most according to claim 4, it is characterised in that the described long 12-16 of control stripes bar
Mm, wide 3-6mm, point yellow, redness, rose band, the longest 2-4mm of each vitta.
6. the preparation method of the test kit detecting helicobacter pylori, it is characterised in that comprise the following steps:
(1) using glacial acetic acid regulation water pH value is 6.5-6.9, and infiltration absorbent cotton is to neutral, standby after dehydrate；
(2) absorbent cotton after bamboo let one ends wound step (1) processes, sterilization, prepare cotton swab；
(3) by carbamide and phenol red pulverizing, mix homogeneously, being dissolved in water into pasty solutions, described carbamide, phenol red and water mass ratio are 1000:1.0-1.5:250-350, each plastics barreled 0.15-0.25 mL solution, are dried, seal；
(4) plastic bag vacuum is used to seal cotton swab, control stripes bar and Plastic Drum, sterilizing, obtain test kit.
The preparation method of the test kit of detection helicobacter pylori the most according to claim 6, it is characterised in that step (2) described bamboo let specification is diameter 1.5-2 mm, long 40-50mm, a diameter of 2.5-3.5 mm of the absorbent cotton of winding, long 5-6 mm.
8. according to the preparation method of the test kit detecting helicobacter pylori described in claim 6 or 7, it is characterised in that step (3) described Plastic Drum diameter 4.5-5.5
Mm, a height of 13-16 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242501.XA CN105755105A (en) | 2016-04-19 | 2016-04-19 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242501.XA CN105755105A (en) | 2016-04-19 | 2016-04-19 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
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| Publication Number | Publication Date |
|---|---|
| CN105755105A true CN105755105A (en) | 2016-07-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610242501.XA Pending CN105755105A (en) | 2016-04-19 | 2016-04-19 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106468663A (en) * | 2016-11-11 | 2017-03-01 | 天益健康科学研究院（镇江）有限公司 | A kind of helicobacter pylori quick detection coupon |
| CN110044890A (en) * | 2019-04-27 | 2019-07-23 | 谱尼测试集团北京科学技术研究院有限公司 | A kind of helicobacter pylori quick screening method |
| CN110455788A (en) * | 2019-06-05 | 2019-11-15 | 谱尼测试集团北京科学技术研究院有限公司 | The rapid detection method of urease activity in a kind of sxemiquantitative saliva |
| CN110527733A (en) * | 2018-05-25 | 2019-12-03 | 上海奥奈特生物科技有限责任公司 | The primer and kit of one group of Visual retrieval helicobacter pylori |
| CN114277086A (en) * | 2021-12-28 | 2022-04-05 | 南京康容健康科技有限公司 | Helicobacter pylori rapid detection reagent |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266461A (en) * | 1998-04-03 | 2000-09-13 | 李钟和 | Composition, kit, and method for detecting helicobacter pylori in biopsy |
| US6677129B1 (en) * | 1998-07-22 | 2004-01-13 | Richard S. Blume | Method for detecting Helicobacter pylori infection |
| CN1611938A (en) * | 2003-10-27 | 2005-05-04 | 上海惠泰医疗科技公司 | Pyloric spirillum rapid diagnosis reagent |
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
-
2016
- 2016-04-19 CN CN201610242501.XA patent/CN105755105A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266461A (en) * | 1998-04-03 | 2000-09-13 | 李钟和 | Composition, kit, and method for detecting helicobacter pylori in biopsy |
| US6677129B1 (en) * | 1998-07-22 | 2004-01-13 | Richard S. Blume | Method for detecting Helicobacter pylori infection |
| CN1611938A (en) * | 2003-10-27 | 2005-05-04 | 上海惠泰医疗科技公司 | Pyloric spirillum rapid diagnosis reagent |
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106468663A (en) * | 2016-11-11 | 2017-03-01 | 天益健康科学研究院（镇江）有限公司 | A kind of helicobacter pylori quick detection coupon |
| CN110527733A (en) * | 2018-05-25 | 2019-12-03 | 上海奥奈特生物科技有限责任公司 | The primer and kit of one group of Visual retrieval helicobacter pylori |
| CN110044890A (en) * | 2019-04-27 | 2019-07-23 | 谱尼测试集团北京科学技术研究院有限公司 | A kind of helicobacter pylori quick screening method |
| CN110455788A (en) * | 2019-06-05 | 2019-11-15 | 谱尼测试集团北京科学技术研究院有限公司 | The rapid detection method of urease activity in a kind of sxemiquantitative saliva |
| CN114277086A (en) * | 2021-12-28 | 2022-04-05 | 南京康容健康科技有限公司 | Helicobacter pylori rapid detection reagent |
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Application publication date: 20160713 |