CN1609195A - Dendritic cell culturing method and kit - Google Patents
Dendritic cell culturing method and kit Download PDFInfo
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- CN1609195A CN1609195A CN 200310107994 CN200310107994A CN1609195A CN 1609195 A CN1609195 A CN 1609195A CN 200310107994 CN200310107994 CN 200310107994 CN 200310107994 A CN200310107994 A CN 200310107994A CN 1609195 A CN1609195 A CN 1609195A
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Images
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Abstract
Description
Prescription | rhIL-4 | ?hSCF | ?hGM-CSF |
Prescription 1a | ?1 | ?1 | ?3 |
Prescription 2a | ?0.8 | ?1.2 | ?3 |
Prescription 3a | ?1.2 | ?0.8 | ?3 |
Prescription 4a | ?1 | ?0.9 | ?3.5 |
Prescription 5a | ?1.1 | ?1.1 | ?2.5 |
Prescription | Gamma-interferon | The humanTNF- | People Hsp70 |
Prescription 1b | ?1 | ?1 | ?8 |
Prescription 2b | ?0.8 | ?1.2 | ?8 |
Prescription 3b | ?1.2 | ?0.8 | ?8 |
Prescription 4b | ?1 | ?0.9 | ?10 |
Prescription 5b | ?1.1 | ?1.1 | ?6 |
Calcium chloride (CaCl 2) | ????116.60 |
Copper sulfate (CuSO 4·5H 2O) | ????0.0013 |
Iron nitrate (Fe (NO 3) 3·9H 2O) | ????0.05 |
Ferrous sulfate (FeSO 4·7H 2O) | ????0.417 |
Repone K (KCl) | ????311.80 |
Magnesium chloride (MgCl 2) | ????28.64 |
Sal epsom (MgSO 4) | ????48.84 |
Sodium-chlor (NaCl) | ????6995.50 |
Sodium bicarbonate (NaHCO 3) | ????2438.00 |
SODIUM PHOSPHATE, MONOBASIC. (NaH 2PO 4·H 2O) | ????62.50 |
Sodium phosphate dibasic (Na 2HPO 4) | ????71.02 |
Zinc sulfate (ZnSO 4·7H 2O) | ????0.432 |
Glucose | ????3151.00 |
Xanthoglobulin sodium salt (Hypoxanthine-Na) | ????2.39 |
Linolic acid (Linoleic Acid) | ????0.042 |
Thioctic Acid (Lipoic Acid) | ????0.105 |
Phenol red (Phenol red) | ????8.10 |
Carnitine (Putresc ine-2HCl) | ????0.081 |
Sodium.alpha.-ketopropionate (Sodium Pyruvate) | ????55.00 |
Thymidine (Thymidine) | ????0.365 |
The L-L-Ala | ????4.45 |
L-arginine hydrochloride (L-Arginine hydrochloride) | ????147.50 |
L-aspartic acid (L-Asparagine-H 2O) | ????7.50 |
L-aspartic acid (L-Aspartic acid) | ????6.65 |
L-halfcystine (L-Cysteine-HCl-H 2O) | ????17.56 |
L-Gelucystine (L-Cystine-2HCl) | ????31.29 |
L-L-glutamic acid (L-Glutamic acid) | ????7.35 |
L-glutaminate (L-Glutamine) | ????365.00 |
G glycine (Glycine) | ????18.75 |
L-Histidine (L-Histidine-HCl-H 2O) | ????31.48 |
L-Isoleucine (L-Isoleucine) | ????54.47 |
L-leucine (L-Leucine) | ????59.05 |
L-lysine hydrochloride (L-Lysine hydrochloride) | ????91.25 |
L-methionine(Met) (L-Methionine) | ????17.24 |
L-phenylamino acid (L-Phenylalanine) | ????35.48 |
L-proline(Pro) (L-Proline) | ????17.25 |
L-Serine (L-Serine) | ????26.25 |
L-Threonine (L-Threonine) | ????53.45 |
L-tryptophane (L-Tryptophan) | ????9.02 |
L-Sodium L-tyrosinate (L-Tyrosine-2Na-2H 2O) | ????55.79 |
L-Xie Ansuan (L-Valine) | ????52.85 |
VITAMIN (VITAMINS) | |
Vitamin H (Biotin) | ????0.0035 |
D-calcium pantothenate (D-Calcium pantothenate) | ????2.24 |
Choline chloride 60 (Choline chloride) | ????8.98 |
Folic acid (Folic acid) | ????2.65 |
I-inositol (i-Inositol) | ????12.60 |
Niacinamide (Niacinamide) | ????2.02 |
Vitamin B6 hydrochloride (Pyridoxine hydrochloride) | ????2.00 |
Riboflavin (Riboflavin) 0.219 | |
Thiamine hydrochloride (Thiamine hydrochloride) | ????2.17 |
Vitamin B12 (Vitamin B12) | ????0.68 |
The insulin human | ????10 |
Human transferrin | ????5.5 |
The recombinant human albumin | ????0.1 |
Cholesterol | ????0.5 |
????Pluronic?F-68 | ????1 |
Dextran | ????1M |
Selenium | ????0.005 |
Claims (10)
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CN101085983A (en) * | 2006-06-06 | 2007-12-12 | 刘永详 | Autologous stem cell, target cell transformed therefrom and its uses |
CN104498434A (en) * | 2014-12-04 | 2015-04-08 | 北京益诺勤生物技术有限公司 | Preparation method of large number of dendritic cells and obtained dendritic cells |
CN105670994A (en) * | 2016-02-28 | 2016-06-15 | 深圳爱生再生医学科技有限公司 | DC (dendritic cell) inducer and application thereof |
CN106282111A (en) * | 2016-08-30 | 2017-01-04 | 孟玲 | A kind of Dendritic Cells Induced factor composition |
CN113249322A (en) * | 2021-05-18 | 2021-08-13 | 蓝莲(杭州)生物科技有限公司 | Culture solution and culture method for DC cells |
Family Cites Families (2)
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JP2005501550A (en) * | 2001-08-30 | 2005-01-20 | スリーエム イノベイティブ プロパティズ カンパニー | Maturation of plasmacytoid dendritic cells using immune response modifier molecules |
CN1408850A (en) * | 2001-09-18 | 2003-04-09 | 王虹 | Immortal technology for human blood dentritic cell |
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Cited By (6)
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CN101085983A (en) * | 2006-06-06 | 2007-12-12 | 刘永详 | Autologous stem cell, target cell transformed therefrom and its uses |
CN104498434A (en) * | 2014-12-04 | 2015-04-08 | 北京益诺勤生物技术有限公司 | Preparation method of large number of dendritic cells and obtained dendritic cells |
CN104498434B (en) * | 2014-12-04 | 2018-01-09 | 北京益诺勤生物技术有限公司 | A kind of preparation method of a large amount of BMDCs, gained BMDC |
CN105670994A (en) * | 2016-02-28 | 2016-06-15 | 深圳爱生再生医学科技有限公司 | DC (dendritic cell) inducer and application thereof |
CN106282111A (en) * | 2016-08-30 | 2017-01-04 | 孟玲 | A kind of Dendritic Cells Induced factor composition |
CN113249322A (en) * | 2021-05-18 | 2021-08-13 | 蓝莲(杭州)生物科技有限公司 | Culture solution and culture method for DC cells |
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