CN1603828A - Surface plasma resonance rapid detection method for paralytic shellfish poisoning - Google Patents
Surface plasma resonance rapid detection method for paralytic shellfish poisoning Download PDFInfo
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Abstract
This invention discloses a paralysis shellfish poison surface plasm resonance rapid measurement, which belongs to environment chemistry and biology molecule detecting field. The method is the following: to add quantitative PSP anti-body to the PSP sample solvent; then to flow on the gold sputtered plate with PSP solidified; to measure the over PSP anti-body in the solvent by use of plasm resonance sensor SPR detector to indirectly measure the concentration of the PSP solvent. This method is especially applied in quantitative study and regular or emergency monitor of PSP in freshwater algae or sea red tide.
Description
Technical field
The invention belongs to environmental chemistry and biomolecule detection field, be particularly related to and adopt surface plasma body resonant vibration (SPR) technology, detect the surface plasma resonance rapid detection method of a kind of paralytic shellfish poison of PSP content in the solution by the surplus of paralytic shellfish poison (PSP) antibody in the test sample solution.
Background technology
Red tide is a kind of global marine environment disaster, and the generation of red tide has constituted serious threat to the marine eco-environment, ocean resources and public health, and resource that it causes and economic loss are difficult to estimation especially.In the toxin that harmful algal produces, with paralytic shellfish poison (PSP) toxin worldwide distribute the widest, harm is maximum.PSP has high specific toxicity to biological nervous system and cardiovascular system, is highly narrow spectrum cell membrane sodium ion channel blocker, can block the excited and conduction of neurocyte, cause poisoner's muscular paralysis in 24h, expiratory dyspnea, anoxic stupor, so that suffocate and die.Since the detected first affirmation of nineteen thirty-seven PSP, PSP worldwide causes comparatively serious economy loss and has caused bigger public health risk.
In recent years, China's immediate offshore area red tide disaster takes place frequently, and in the East Sea and marine site, the South Sea, reports once that too much cause had eaten the poisoning that the contamination shellfish causes.China ocean administrative responsibile institution has strengthened red tide monitoring and management work dynamics for this reason, and from over 2002, multiple district has set up 11 red tide monitored spaces at national coastal waters red tide, and red tide monitoring work has been in full swing.But, monitoring technology falls behind the bottleneck that has become China's red tide monitoring work relatively at present, the working practice of red tide monitoring presses for fast, sensitive, workable and detection method that popularize in marine monitoring basic unit easily, with the routine and the emergency monitoring work of the satisfied red tide disaster that takes place frequently day by day, and the strict day by day culture fishery food security and the demand of detection.Therefore, set up sensitive, PSP detection method efficiently, have important practice significance for the work such as edible safety of red tide monitoring and marine product.
At present, being used for the method that PSP detects mainly contains: bio-toxicity detection technique, chemical detection technique and biological chemistry detection technique etc.
(1) bio-toxicity detection technique
Mainly be meant semiquantitative small white mouse acute toxicity evaluation assessment, because this method is easy and simple to handle, for many years always by the PSP conventional sense method of many countries as standard.But this method is consuming time, the changeability height, and sensitivity is low, is subjected to sample quantitative limitation to be checked, can not provide deterministic evidence to particular toxin, and the extremely dispute owing to use living animal to test.
(2) chemical detection technique
The chemical technology that has been successfully applied to the PSP detection by quantitative mainly contains: methods such as oxidizing process, LC (liquid chromatography)-MS (mass spectrum) method, LC-MS-MS method before fluorescence derivatization method, the HPLC-post behind HPLC (high performance liquid chromatography)-post.The preceding oxidizing process of fluorescence derivatization method and HPLC-post is that report uses maximum PSP detection methods at present behind the HPLC-post, because of its accommodation wide, enjoy popularization, but the defective of this class methods maximum needs large-scale instrument and equipment when being to test, very high to the operators'skills requirement, testing cost is high simultaneously.The LC-MS method is higher because of its experimentation cost, and the research of being carried out is not a lot.
(3) biological chemistry detection technique
The biological chemistry detection technique mainly comprises electrophysiological technique, phosphoprotein phosphatase 2A (PP2A) inhibition analysis and enzyme linked immunosorbent assay (ELISA) etc.ELISA becomes the focus of present research owing to its high sensitivity.But the elisa technique analysis cost is also higher relatively, and particularly the reagent of its use needs mark, thereby has reduced its operability, is not used widely as yet.
Summary of the invention
The object of the present invention is to provide a kind of paralytic shellfish poison's surface plasma resonance rapid detection method, utilization has the SPR detector of surface plasma resonance sensor, incide prism and be plated on the interface between the golden film of prism surface with detection laser, marked change takes place in catoptrical light intensity and phase place immediately, thus the PSP content in the indirect determination testing sample.It is characterized in that: utilize one deck glucosan to solidify PSP spattering the glass surface that golden film is arranged, and in PSP solution to be detected, add the PSP antibody of quantitative concentration fixed, detect not combined PSP antibody in the solution by the SPR detector, thus the PSP content in the indirect determination testing sample.The concrete operations step is as follows:
(1) preparation of sensing chip and surperficial carboxyphenyl pre-service
A. clean: get the high-flatness ultrathin glass substrate, the adding volume ratio is 3: 1 H
2SO
4And H
2O
2, boil, to H
2SO
4Boiling;
B. sputter: the metallic film of sputter 20nm Cr and 50nm Au on slide;
C. fixing glucosan: earlier with alcohol-water (volume ratio 80: the 20) solution-treated of 16-sulfydryl cetyl alcohol, again with epichlorokydrin fixedly glucosan as the matrix of sensing chip.
(2) antigen is in the curing on sensing chip surface
A. current-carrying: with the current-carrying damping fluid is carrying object, and flow velocity is 5 μ l/min;
B. antigen solidifies: activating solution flows through 10min and carries out surface active with 10 μ l/min flow velocitys, gets the PSP standard solution of 40ppb again, and rare to 10ppb with phosphate buffer normal saline (PBS), with the flow velocity sample introduction 10min of 10 μ l/min, the RU value should reach 200RU;
C. sealing: confining liquid flows through 5min at least with the flow velocity of 10 μ l/min;
(3) extraction of sample: quantitatively take by weighing testing sample, add 0.1M HCl solution, fully stir evenly, regulating pH is 3～4, ultrasonication; Again with the centrifugal 10min of 3000r/m, supernatant is with the F-Kynoar membrane filtration of 0.25-; Filtrate is placed Erlenmeyer flask, and 5min is boiled in 100 ℃ of water-baths, is cooled to room temperature, and regulating pH with the 1M sodium acetate solution is 4.5, and this solution is the PSP extract.
(4) the PSP standard model is measured and data processing
A. current-carrying: as current-carrying, it is 5 μ l/min that flow velocity is set with reaction buffer;
B. calibration curve method detects: the A. typical curve is drawn: get the PSP standard solution of variable concentrations, add the PSP antibody of the same concentrations of same amount at every turn, add reaction buffer solution to testing required volume 50～100 μ l.It is 5 μ l/min that flow velocity is set, and annotates sample 4min, respectively by being solidified with the chip of PSP, record SPR response signal.Then according to being ordinate, be horizontal ordinate drawing standard curve with the concentration of the PSP standard solution of adding with the RU of resonance response unit value.The method of testing of B. pressing typical curve detects the PSP sample, according to its RU value, reads PSP content on typical curve.
PSP standard solution wherein: take by weighing a certain amount of PSP solid, add sample diluting liquid, add secondary water constant volume again, the concentration of preparation Series in PS P standard solution is 0ppb, 2.5ppb, 5ppb, 10ppb, 20ppb, 40ppb.
C. standard addition method detects: get many parts of equal-volume PSP samples to be measured, add quantitative PSP antibody respectively, add the PSP standard solution of variable concentrations again, add reaction buffer solution to testing required volume 50～100 μ l.It is 5 μ l/min that flow velocity is set, and annotates sample 4min, respectively by being solidified with the chip of PSP, record SPR response signal.Being ordinate with resonance response unit (RU) value then, is that horizontal ordinate is made the linear regression curve with the PSP concentration of standard solution that adds, and the absolute value of the intersection point of curve and horizontal ordinate is the concentration of PSP sample to be measured.
Described current-carrying damping fluid is: pH=7.4, and 0.01M HEPES+0.15M NaCl+0.003M EDTA+0.005% polysorbas20 after filtration, after the degassing, is preserved down for+4～8 ℃;
Wherein HEPES is N-2-hydroxyethyl piperazine-N '-2 '-ethylsulfonic acid, and EDTA is an ethylenediamine tetraacetic acid.
Described activating solution is 0.1M NHS+0.4M EDC; Wherein EDC is ethyl-(dimethylamino-propyl) carbodiimide, and NHS is a N-maloyl imines.
Described PSP antibody is taken from paralytic shellfish poison's rabbit source antibody or mouse source antibody.
Described confining liquid is a diethanolamine hydrochloride.
Described sample diluting liquid is a 0.01M HAc-NaAc damping fluid, pH=4.5, aseptic filtration.
Beneficial effect of the present invention is:
(1) the present invention adopts indirect SPR detection method to measure the content of PSP, and by solidifying the PSP molecule, the concentration that the content of residue PSP antibody comes indirect determination PSP in the detection solution has effectively improved sensitivity; Detection time is short, can detect the result in the general 5min; Amount of samples is few, and it is low to detect cost, is convenient to extensive popularization.
(2) the present invention reduces subjectivity by SPR instrument record, can adopt calibration curve method, also can adopt standard addition method, can be applied to the detection of aquatic products and environmental sample; Mass detection can be carried out, the detection of a plurality of samples, a plurality of websites can be accomplished.
(3) measure sensing chip behind the required coupling connection PSP, detect according to the aforesaid operations step, but each the passage duplicate detection PSP sample on each chip is more than 100 times.
Embodiment
The present invention is a kind of paralytic shellfish poison's a surface plasma resonance rapid detection method.It is to utilize the SPR detector that has surface plasma resonance sensor, come the detection laser of surface plasma resonance sensor of the sensor technology of detection molecules interphase interaction to incide prism with the near field optic principle and be plated on the interface between the golden film of prism surface, marked change takes place in catoptrical light intensity and phase place immediately, in the sample solution on acceptor and the chip intensity and the quantity of part combination different, catoptrical intensity and phase place are also different.The variation of sort signal is closely related with the refractive index near the material of metal surface.If the PSP molecule is solidified in the metal surface, so just can PSP antibody molecule free in the solution be detected, thus the PSP content in the indirect determination testing sample.It is characterized in that: utilize one deck glucosan to solidify PSP spattering the glass surface that golden film is arranged, and in PSP solution to be detected, add the PSP antibody of quantitative concentration fixed, detect not combined PSP antibody in the solution by the SPR detector, thus the PSP content in the indirect determination testing sample.The concrete operations step is as follows:
(1) preparation of sensing chip and surperficial carboxyphenyl pre-service
A. clean: get the high-flatness ultrathin glass substrate, the adding volume ratio is 3: 1 H
2SO
4And H
2O
2, boil, to H
2SO
4Boiling;
B. sputter: the metallic film of sputter 20nm Cr and 50nm Au on slide;
C. fixing glucosan: earlier with alcohol-water (volume ratio 80: the 20) solution-treated of 16-sulfydryl cetyl alcohol, again with epichlorokydrin fixedly glucosan as the matrix of sensing chip.
(2) antigen is in the curing on sensing chip surface
A. current-carrying: with the current-carrying damping fluid is carrying object, and flow velocity is 5 μ l/min;
B. antigen solidifies: activating solution flows through 10min and carries out surface active with 10 μ l/min flow velocitys, gets the PSP standard solution of 40ppb again, and rare to 10ppb with phosphate buffer normal saline (PBS), with the flow velocity sample introduction 10min of 10 μ l/min, the RU value should reach 200RU;
C. sealing: confining liquid flows through 5min at least with the flow velocity of 10 μ l/min;
(3) extraction of sample: quantitatively take by weighing testing sample, add 0.1M HCl solution, fully stir evenly, regulating pH is 3～4, ultrasonication; Again with the centrifugal 10min of 3000r/m, supernatant is with the F-Kynoar membrane filtration of 0.25 μ m; Filtrate is placed Erlenmeyer flask, and 5min is boiled in 100 ℃ of water-baths, is cooled to room temperature, and regulating pH with the 1M sodium acetate solution is 4.5, and this solution is the PSP extract.
(4) the PSP standard model is measured and data processing
A. current-carrying: as current-carrying, it is 5 μ l/min that flow velocity is set with reaction buffer;
B. calibration curve method detects: the A. typical curve is drawn: get the PSP standard solution of variable concentrations, add quantitative PSP antibody, add reaction buffer solution to testing required volume.It is 5 μ l/min that flow velocity is set, and annotates sample 4min, respectively by being solidified with the chip of PSP, record SPR response signal.According to being ordinate, be horizontal ordinate drawing standard curve then with the standard P SP concentration that adds with the RU of resonance response unit value.The method of testing of B. pressing typical curve detects the PSP sample, according to its RU value, reads PSP content on typical curve.
PSP standard solution wherein: take by weighing a certain amount of PSP solid, add sample diluting liquid, add secondary water constant volume again, the concentration of the series standard solution of preparation is 0ppb, 2.5ppb, 5ppb, 10ppb, 20ppb, 40ppb.
C. standard addition method detects: get many parts of equal-volume PSP samples to be measured, add quantitative PSP antibody respectively, add the PSP standard solution of above-mentioned concentration again, add reaction buffer solution to testing required volume 50～100 μ l.It is 5 μ l/min that flow velocity is set, and annotates sample 4min, respectively by being solidified with the chip of PSP, record SPR response signal.Being ordinate with resonance response unit (RU) value then, is that horizontal ordinate is made the linear regression curve with the PSP concentration of standard solution that adds, and the absolute value of the intersection point of curve and horizontal ordinate is the concentration of PSP sample to be measured.
Described current-carrying damping fluid is: pH=7.4, and 0.01M HEPES+0.15M NaCl+0.003M EDTA+0.005% polysorbas20 after filtration, after the degassing, is preserved down for+4～8 ℃;
Wherein HEPES is N-2-hydroxyethyl piperazine-N '-2 '-ethylsulfonic acid, and EDTA is an ethylenediamine tetraacetic acid.
Described activating solution is 0.1M NHS+0.4M EDC; Wherein EDC is ethyl-(dimethylamino-propyl) carbodiimide, and NHS is a N-maloyl imines.
Described confining liquid is a diethanolamine hydrochloride.
Described PSP antibody is taken from paralytic shellfish poison's rabbit source antibody (the anti-PSP of rabbit) or mouse source antibody (mouse-anti PSP).
Described sample diluting liquid is a 0.01M HAc-NaAc damping fluid, pH=4.5, aseptic filtration.
Utilize the high sensitivity of SPR sensing technology and the characteristics of high selectivity, overcome the shortcoming and defect of existing PSP detection method, a kind of highly sensitive, quick, indirect SPR detection method that can detect PSP in food and environmental monitoring field on a large scale is provided.
Claims (9)
1. a paralytic shellfish poison surface plasma resonance rapid detection method utilizes surface plasma body resonant vibration SPR detector, detects the light intensity and the phase change of solution and gold-plated glass interface total reflection light, thus the PSP content in the indirect determination testing sample; It is characterized in that: spattering glass surface elder generation curing one deck glucosan that golden film is arranged, solidify PSP then, each PSP antibody that in PSP solution to be detected, adds quantitative same concentrations, detect not combined PSP antibody in the solution by the SPR detector, thus the PSP content in the indirect determination testing sample; The concrete operations step is as follows:
(1) preparation of sensing chip and surperficial carboxyphenyl pre-service
A. clean: get the high-flatness ultrathin glass substrate, the adding volume ratio is 3: 1 H
2SO
4And H
2O
2, boil, to H
2SO
4Boiling;
B. sputter: the metallic film of sputter 20nm Cr and 50nm Au on slide;
C. fixing glucosan: earlier with alcohol-water (volume ratio 80: the 20) solution-treated of 16-sulfydryl cetyl alcohol, again with epichlorokydrin fixedly glucosan as the matrix of sensing chip;
(2) antigen is in the curing on sensing chip surface
A. current-carrying: with the current-carrying damping fluid is carrying object, and flow velocity is 5 μ l/min;
B. antigen solidifies: activating solution flows through 10min and carries out surface active with 10 μ l/min flow velocitys, gets the PSP standard solution of 40ppb again, and rare to 10ppb with phosphate buffer normal saline (PBS), with the flow velocity sample introduction 10min of 10 μ l/min, the RU value should reach 200RU;
C. sealing: confining liquid flows through 5min at least with the flow velocity of 10 μ l/min;
(3) extraction of sample: quantitatively take by weighing testing sample, add 0.1M HCl solution, fully stir evenly, regulating pH is 3～4, ultrasonication; Again with the centrifugal 10min of 3000r/m, supernatant is with the F-Kynoar membrane filtration of 0.25 μ m; Filtrate is placed Erlenmeyer flask, and 5min is boiled in 100 ℃ of water-baths, is cooled to room temperature, and regulating pH with the 1M sodium acetate solution is 4.5, and this solution is the solution after PSP extracts;
(4) the PSP standard model is measured and data processing
A. current-carrying: as current-carrying, it is 5 μ l/min that flow velocity is set with reaction buffer;
B. calibration curve method detects: the A. typical curve is drawn: get the PSP standard solution of variable concentrations, add the PSP antibody of the same concentrations of same amount at every turn, add reaction buffer solution again to testing required volume 30～50 μ l; It is 5 μ l/min that flow velocity is set, annotate sample 4min, respectively by being solidified with the chip of PSP, record SPR response signal, then according to being ordinate with the RU of resonance response unit value, be horizontal ordinate drawing standard curve with the concentration of the PSP standard solution that adds, B. presses the method for testing detection PSP sample of typical curve, according to its RU value, on typical curve, read PSP content;
Wherein the compound method of PSP standard solution is: take by weighing a certain amount of PSP solid, add sample diluting liquid, add secondary water constant volume again, the concentration of preparation Series in PS P standard solution is 0ppb, 2.5ppb, 5ppb, 10ppb, 20ppb, 40ppb;
C. standard addition method detects: get many parts of equal-volume PSP samples to be measured, add quantitative PSP antibody respectively, the PSP standard solution that adds above-mentioned concentration again, add reaction buffer solution to testing required volume 30～50 μ l, it is 5 μ l/min that flow velocity is set, annotate sample 4min, respectively by being solidified with the chip of PSP, record SPR response signal; Being ordinate with resonance response unit (RU) value then, is the horizontal ordinate mapping with the PSP concentration of standard solution that adds, and the line linearity of going forward side by side returns, and calculates the concentration of PSP sample to be measured by regression curve.
2. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: when PSP is cured to coupling and is associated with the sensing chip of glucosan, PSP will dilute with the phosphate buffer normal saline (PBS) of pH=7.5, and concentration is 10ppb.
3. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: use when being solidified with the chip detection PSP antibody of PSP, sample will be used 0.01M HAc-NaAc damping fluid+0.15M NaCl+0.005% polysorbas20, the reaction buffer preparation of pH=4.5.
4. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: the PSP antibody that detects on the chip of back need be with 0.01M NaOH solution as eluent.
5. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: in the described sample extraction method, it is 4.5 with pH regulator that the sample before detecting needs with the 1M sodium acetate solution.
6. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: described current-carrying damping fluid is: pH=7.4, and 0.01M HEPES+0.15M NaCl+0.003MEDTA+0.005% polysorbas20, after filtration, after the degassing, preserve down for+4～8 ℃.
7. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: described activating solution is 0.1M NHS+0.4M EDC; Wherein EDC is ethyl-(dimethylamino-propyl) carbodiimide, and NHS is a N-maloyl imines.
8. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: described confining liquid is a diethanolamine hydrochloride.
9. according to the described paralytic shellfish poison's of claim 1 surface plasma resonance rapid detection method, it is characterized in that: described reaction buffer (pH=4.5): 0.01M HAc-NaAc damping fluid+0.15M NaCl+0.005% polysorbas20;
Wherein HEPES is N-2-hydroxyethyl piperazine-N '-2 '-ethylsulfonic acid, and EDTA is an ethylenediamine tetraacetic acid.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101858833A (en) * | 2010-05-21 | 2010-10-13 | 王秋艳 | Paralytic shellfish poisoning (PSP) standard sample and preparation method and application thereof |
| CN101963617A (en) * | 2010-05-26 | 2011-02-02 | 江西中烟工业有限责任公司 | Novel indirect inhibition immunoassay method for measuring nicotine in body fluid |
| CN102312019A (en) * | 2011-06-09 | 2012-01-11 | 中国人民解放军第三军医大学第一附属医院 | Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection |
| CN103323429A (en) * | 2013-05-30 | 2013-09-25 | 中南大学 | Surface plasmon resonance (SPR) sensor chip for detection of melamine content of milk and its preparation method and detection method |
| CN103884682A (en) * | 2014-03-20 | 2014-06-25 | 暨南大学 | Surface plasma resonance biochip, and preparation method and application thereof |
| CN106908418A (en) * | 2017-03-24 | 2017-06-30 | 北京大学 | Hesperidine and the horizontal assay method of protein binding based on surface plasma body resonant vibration |
| CN107064071A (en) * | 2017-03-24 | 2017-08-18 | 北京大学 | Determine rutin and the surface plasma body resonant vibration analysis method of protein binding level |
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| CN108291909A (en) * | 2015-04-28 | 2018-07-17 | 奥菲迪亚有限公司 | Analyze analyte detection and its method |
| CN110609139A (en) * | 2018-06-14 | 2019-12-24 | 深圳市理邦精密仪器股份有限公司 | Antigen concentration excess detection method, device and storage medium |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0857972A1 (en) * | 1997-01-24 | 1998-08-12 | Tepual, S.A. | Immunoassay for the detection and quantitation of toxins causing paralytic shellfish poisoning |
| CN1218180C (en) * | 2001-11-23 | 2005-09-07 | 上海数康生物科技有限公司 | Surface plasma resonance biosensor for detecting several biological signals parallelly |
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2004
- 2004-10-20 CN CNB2004100840617A patent/CN100399027C/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101858833A (en) * | 2010-05-21 | 2010-10-13 | 王秋艳 | Paralytic shellfish poisoning (PSP) standard sample and preparation method and application thereof |
| CN101963617A (en) * | 2010-05-26 | 2011-02-02 | 江西中烟工业有限责任公司 | Novel indirect inhibition immunoassay method for measuring nicotine in body fluid |
| CN101963617B (en) * | 2010-05-26 | 2013-07-10 | 江西中烟工业有限责任公司 | Novel indirect inhibition immunoassay method for measuring nicotine in body fluid |
| CN102312019A (en) * | 2011-06-09 | 2012-01-11 | 中国人民解放军第三军医大学第一附属医院 | Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection |
| CN102312019B (en) * | 2011-06-09 | 2013-03-27 | 中国人民解放军第三军医大学第一附属医院 | Biological chip for rapidly detecting pre-pregnant intrauterine toxoplasma gondii and cytomegalovirus infection |
| CN103323429A (en) * | 2013-05-30 | 2013-09-25 | 中南大学 | Surface plasmon resonance (SPR) sensor chip for detection of melamine content of milk and its preparation method and detection method |
| CN103884682A (en) * | 2014-03-20 | 2014-06-25 | 暨南大学 | Surface plasma resonance biochip, and preparation method and application thereof |
| CN103884682B (en) * | 2014-03-20 | 2016-09-07 | 暨南大学 | Surface plasma body resonant vibration biochip and preparation method and application |
| CN108291909A (en) * | 2015-04-28 | 2018-07-17 | 奥菲迪亚有限公司 | Analyze analyte detection and its method |
| CN108291909B (en) * | 2015-04-28 | 2022-07-12 | 森佐健康有限公司 | Analyte detection and methods thereof |
| CN106908418A (en) * | 2017-03-24 | 2017-06-30 | 北京大学 | Hesperidine and the horizontal assay method of protein binding based on surface plasma body resonant vibration |
| CN107064071A (en) * | 2017-03-24 | 2017-08-18 | 北京大学 | Determine rutin and the surface plasma body resonant vibration analysis method of protein binding level |
| CN107101977A (en) * | 2017-03-24 | 2017-08-29 | 北京大学 | Determine Quercetin and the surface plasma body resonant vibration analysis method of protein binding level |
| CN110609139A (en) * | 2018-06-14 | 2019-12-24 | 深圳市理邦精密仪器股份有限公司 | Antigen concentration excess detection method, device and storage medium |
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