CN1603826A - Rapid immune assay test paper batchmeter and quantitative method thereof - Google Patents
Rapid immune assay test paper batchmeter and quantitative method thereof Download PDFInfo
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- CN1603826A CN1603826A CN 03154445 CN03154445A CN1603826A CN 1603826 A CN1603826 A CN 1603826A CN 03154445 CN03154445 CN 03154445 CN 03154445 A CN03154445 A CN 03154445A CN 1603826 A CN1603826 A CN 1603826A
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Abstract
This invention relates to a rapid immune test paper quantitative machine and its method , which is the following: to use an image acquisition unit to get a digital image from the rapid immune test paper and to choose at least one main characteristics from the digital image and input the information to the quantitative unit of neutral net for quantitative compution and to output the compution result to the output unit.
Description
Technical field
The invention relates to quantitative machine of a kind of rapid immunochromatographic test test paper and quantivative approach thereof, refer in particular to a kind of principal character of utilizing optical image technology acquisition rapid immunochromatographic test test paper, and with this principal character after the neural network dosing unit quantitative Analysis of core technology, obtain a certain amount of result, and be presented at the quantitative machine of rapid immunochromatographic test test paper and the quantivative approach thereof of output unit.
Background of invention
Tachysynthesis is tested and inspected paper (Rapid Immunochromatographic Test) is a kind of immunochromatography principle of utilizing, come the test paper of colour generation with colloidal gold particle, surface-coated one deck monoclonal antibody of this kind test paper, antigen or protein to be measured in this monoclonal antibody and blood sample or the blood serum sample have selectivity. therefore, when obtain blood sample or blood serum sample and will it after on the test paper, liquid in the sample will move up along test paper along with colloidal gold particle utilizes the capillary principle, when it arrived the detection test result line (Test Line) of this test paper, any determined antigen or protein in this blood sample or blood serum sample all can be booked and colour generation; This blood sample or blood serum sample can continue to move and colour generation toward the verification test product pipeline (Control Line) of this test paper afterwards.When this verification test product pipeline colour generation appearance, expression blood sample or the reaction of blood serum sample on test paper are finished, if verification test result line and verification test product pipeline all do not have colour generation, then represent the verification test failure.
And the interpretation of the verification test result of rapid immunochromatographic test test paper at present, be etc. that verification test result line and verification test product pipeline are when occurring, naked eyes utilize the depth of the color behind the pipeline colour generation to judge verification test result line and verification test product, even the verification test result line is deeply felt than verification test QC line color and is shown that antigen to be measured or protein concentration are higher than verification test product pipeline, if the verification test result line color is shallow, represent that then antigen to be measured or protein concentration are lower than verification test product pipeline; Yet; this interpretation mode only can be carried out the interpretation of sxemiquantitative (Semi-Quantitation); make this rapid immunochromatographic test test paper can only be used to judge normal or undesired, high concentration or low concentration, and can't obtain the result of a quantitative data (Quantitation).
Therefore the inventor is in line with the positive spirit of invention, research and develop a kind ofly can solve instrument and the method that known rapid immunochromatographic test test paper can only carry out sxemiquantitative judgement defective, several after deliberation, experiment, whole to finishing the present invention, in the hope of Jiahui common people in view of the above
Summary of the invention
One of fundamental purpose of the present invention is to provide quantitative machine of a kind of rapid immunochromatographic test test paper and quantivative approach thereof, in the hope of carrying out interpretation and calculating to the result after the military test paper test of rapid immunochromatographic test, from obtaining quantitative data.
One of fundamental purpose of the present invention also is to provide quantitative machine of a kind of rapid immunochromatographic test test paper and quantivative approach thereof, can carry out the interpretation and the calculating of its test result at various rapid immunochromatographic test test paper, and obtain its quantitative data.
One of fundamental purpose of the present invention also is to provide quantitative machine of a kind of rapid immunochromatographic test test paper and quantivative approach thereof, can upgrade quantitatively required image feature at any time.
The quantitative machine of rapid immunochromatographic test test paper of the present invention comprises:
One image acquisition unit utilizes the general expression image technology of picking to obtain the digitized video of a rapid immunochromatographic test test paper;
One feature extraction unit, utilize the digitized video of at least one quantitative characteristic on the digitized video handling procedure acquisition rapid immunochromatographic test test paper, this a certain amount of feature can be test paper verification test product pipeline, test paper is picked up test test result line ... wait the image feature on the rapid immunochromatographic test test paper;
One neural network dosing unit, it has a plurality of cognitive time networks of the plasticity finished of having trained, and network is with the weighted value of error back algorithm roll-off network each time, simultaneously, and the framework of being formed with the several network, as the core technology of quantitative Analysis;
One storage element is to store plural number to plant parameter value, as weighted value, critical value and system's related parameter values of a plurality of eigenwerts, a plurality of networks.
One output unit, it has LCD, light emitting diode, loudspeaker, exportable quantitative data result.
According to technical scheme provided by the invention, utilize image acquisition unit to obtain the digitized video of rapid immunochromatographic test test paper, and on it, capture a certain amount of at least special efficacy digitized video by feature extraction unit, again through the computing in addition of neural network dosing unit, and carry out quantitatively exporting a certain amount of data result after the computing to output unit at last.
Rapid immunochromatographic test test paper quantivative approach of the present invention comprises the following steps:
(A) provide the rapid immunochromatographic test test paper of a specified quantitative;
(B) this test paper is inserted an image acquisition unit, to obtain the digitized video of this test paper;
(C) digitized video to the test paper of obtained specified quantitative carries out preliminary interpretation, and this is treated whether quantitative test paper is that Validity Test and PRELIMINARY RESULTS are judged;
(D) utilize a feature extraction unit to capture at least one principal character in the digitized video of this test paper;
(E) see through a neural network dosing unit principal character of the digitized video that captured is carried out quantitative Analysis;
(F) will calculate the resulting quantitative data result in back and export output unit to.
Description of drawings
Fig. 1: structure calcspar of the present invention;
Fig. 2: the synoptic diagram of plasticity cognition network framework of the present invention;
Fig. 3: the synoptic diagram of error back formula neural network of the present invention;
Fig. 4: the action flow chart of quantivative approach of the present invention;
Fig. 5: the entity synoptic diagram of the quantitative machine of rapid immunochromatographic test test paper of the present invention.
The figure number explanation:
(1) image acquisition unit
(2) feature extraction unit
(3) neural network dosing unit
(31) the inferior network of the inferior network of the inferior network of inferior network (32) (33) (34)
(4) storage element
(5) output unit
(51) liquid crystal indication device (52) light emitting diode (53) loudspeaker
(61) check test paper inlet
(5) the quantitative machine of rapid immunochromatographic test test paper
(62) test paper outlet
(63) image capture unit
Embodiment
At first, see also shown in Figure 1ly, the quantitative machine of rapid immunochromatographic test test paper of the present invention mainly is made up of an image acquisition unit (1), a feature extraction unit (2), a neural network dosing unit (3), a storage element (4) and an output unit (5).This image acquisition unit (1) is to be used for obtaining the digitized video for the treatment of quantitative rapid immunochromatographic test test paper; In present embodiment, this image acquisition unit (1) can be any optical image capture device, the preferably is general optical scanner, for example: paper feed type optical scanner or branch master mode high speed scanner, the best is backlight type charge coupled cell (ChargeCoupled Device, CCD) or complementary metal oxide silicon (Complementary Metal-oxideSemiconductor, CMOS) image capture unit.Wherein, the speed of digitized video that must get the rapid immunochromatographic test test paper with backlight type charge coupled cell (CCD) or complementary metal oxide silicon (CM0OS) image capture unit is more a lot of soon than the speed of general optical scanner, therefore if adopt backlight type charge coupled cell (CCD) or complementary metal oxide silicon (CMOS) image capture unit can reduce the time of acquisition rapid immunochromatographic test test paper image.This feature extraction unit (2) is the principal character that captures rapid immunochromatographic test test paper digitized video according to a series of digitized video handling procedure, and it is pulled the principal character of getting and can be test paper verification test product pipeline, test paper verification test result line etc.
This neural network dosing unit (3), be to set up the cognitive time network (Plastic Perceptron) (31) of plasticity through a plurality of weighted values and critical value, (32), (33), and utilize several to train the cognitive time network (31) of the plasticity of finishing, (32), (33) framework of being formed (seeing also shown in Figure 2), the cognitive time network (31) of this plasticity, (32), (33) constituted by a plurality of error back neural networks with single hiding layer and single output node, its number is determined by the number of class categories, the cognitive time network (31) of each plasticity, (32), (33) promptly represent a classification, and the cognitive time network (31) of each plasticity, (32), (33) independent mutually between, can parallel dispersion treatment, its hiding node layer is quite few simultaneously, make the cognitive time network (31) of each plasticity, (32), (33) can both restrain (Converge) easily fast, and when increasing by a new cognitive network (34) (increasing another rapid immunochromatographic test test paper classification) of plasticity, can train other plasticity time network (31) again, (32) (33) get final product (as shown in Figure 2) and the cognitive time network (34) of new plasticity need only be added in this network architecture); The training method of the cognitive time network (31) of this plasticity, (32), (33) is to utilize known error back algorithm (Back Propagation)), and the principal character that makes the cognitive time network (31) of each plasticity, (32), (33) come quantitative testing rapid immunochromatographic test test paper with the error back algorithm, the relevant calculation flow process of this error back algorithm then can be joined shown in Figure 3, the steps include:
Step 1: initializes weights value (Weights) and critical value;
Step 2: the output that input template and setting are wanted;
Step 3: the output valve of computational grid reality;
Step 4: adjust weighted value and critical value;
Step 5: get back to step 1, till all weighted values and critical value are stable.
This storage element (4) can be considered a data bank with complex parameter value, and this parameter value comprises weighted value, critical value or system's related parameter values of a plurality of eigenwerts, a plurality of networks.Quantitatively computing be with the image feature data input to the neural network (promptly having configured parameter value) of having trained wherein the calculating of neural network all calculate according to this parameter value (being weighted value).Wherein, each image feature all needs the neural network of different parameters value just can carry out quantitative computing.In this enforcement, these eigenwerts are in order to carry out quantitative foundation as the cognitive time network (31) of each plasticity, (32), (33); As for weighted value and critical value is to be used for setting up an error back algorithm and plasticity cognition network framework for neural network dosing unit (3) through these weighted values and critical value.
This output unit (5), comprise a LCD (LCD)) (51), a plurality of light emitting diode (LED) (52), an and loudspeaker (53), it mainly is after neural network dosing unit (3) exports quantitative result to output unit (5), the loudspeaker (53) of output unit (5) will send the sound of finishing quantitative Analysis, light emitting diode (52) can send light or be the flicker shape, and LCD (51) then shows the data of quantitative result.
Aforesaid feature extraction unit (2) and neural network dosing unit (3) are to be responsible for carrying out by electronics platform such as microprocessor with high speed arithmetic logical operation characteristic or digital signal processor (DSP) wafer set handling and computing: simultaneously, this feature extraction unit (2), neural network dosing unit (3) and storage element (4) can be integrated on the integrated circuit platform.
Consult shown in Figure 4ly, the step of the described rapid immunochromatographic test test paper of present embodiment quantivative approach is:
(A) provide one to treat quantitative rapid immunochromatographic test test paper;
(B) this test paper is inserted an image acquisition unit (1), to obtain the digitized video of this test paper;
(C) digitized video to this test paper carries out preliminary judgement;
(D) utilize a feature extraction unit (2) to capture the digitized video of at least one principal character on this test paper;
(E) see through a neural network dosing unit (3) and come the eigenwert of the principal character digitized video that captured is done computing, and carry out quantitatively;
(F) quantitative result after the computing is exported to output unit (5).
Be example with quantitative first type fetoprotein (Alpha-Fetalprotein) immunity inspection test paper again, and see also the action flow chart of Fig. 4.At first, choose one and treat quantitative rapid immunochromatographic test test paper (step S301)), and this test paper inserted the digitized video (step S302) of image acquisition unit (1) (in the lump with reference to Fig. 1) to obtain the specified quantitative test paper, carry out the PRELIMINARY RESULTS analysis earlier after obtaining the digitized video of test paper, to treat quantitative test paper test test article pipeline and classification of verification test result line and preliminary interpretation; For example: treat that quantitative test paper only contains the feature of single line, then response failure if two line features all have, is then compared according to the verification test result line and the verification test product pipeline depth, responds normal or undesired (step S303).
When its response when undesired, then it exports this up-set condition to output unit (5), makes loudspeaker (53) send the caution sound, caution light takes place in light emitting diode (52), and goes up in LCD (51) and to show error message.
No matter response is for normal or undesired; all utilize feature extraction unit (2) acquisition to treat main quantitative characteristic (step S304) in the digitized video of quantitative test paper again; for example: test paper verification test product pipeline; quantitative characteristics such as test paper verification test result line; and the quantitative characteristic that will capture is delivered to neural network dosing unit (3) and is carried out quantitative Analysis and handle (step S305) and make for a certain amount of result of output to output unit (5); at this moment the loudspeaker (53) in the output unit (5) will send and finish the quantitative sound; light emitting diode (52) sends light or sends flickering light, and LCD (51) then can show the quantitative result data.
Fig. 5 is the entity synoptic diagram that shows the quantitative machine of rapid immunochromatographic test test paper of the present invention (5), show that various immunity inspection test paper are to be sent into by test paper inlet (61), then see through the digitized video that backlight type charge coupled cell (CCD) or complementary metal oxide silicon (CMOS) image capture unit (63) are obtained this rapid immunochromatographic test test paper, for quantitatively, send by test paper outlet (62) more at last.
Certainly, the quantitative machine of rapid immunochromatographic test test paper of the present invention (6) only must be stored to storage element (4) with the parameter value after the training of outside and the parameter value of storage element (4) is adjusted, the cognitive time network (31) (32) of plasticity in training neural network dosing unit (3), (33) then can quantitative different immunity inspection test paper, and for example: various rapid immunochromatographic test test paper such as paper, PSA immunity inspection test paper are inspected in the immunity of first type fetoprotein.Wherein, the training method of cognitive network of plasticity be utilize known error back algorithm (Back, Propagaton).
By above explanation as can be known, the present invention mainly utilizes backlight type charge coupled cell (CCD) or complementary metal oxide silicon (CMOS) image capture unit (63) to obtain the digitized video of the rapid immunochromatographic test test paper of specified quantitative fast, and extract the principal character of this digitized video, utilize error back formula algorithm to finish the cognitive time network (31) of plasticity of training for seeing through one, (32), (33) neural network dosing unit (3) carries out quantitative Analysis to be handled, and after quantitative Treatment is intact, export these quantitative result data to output unit (5), and by the LCD (51) in the output unit (5), the quantitative result of this test paper check of light emitting diode (52) and loudspeaker (53) alert, reach the mode that can see through quantitatively quantification and improve personnel's interpretation accuracy rate, and can quantitative various different rapid immunochromatographic test test paper, and can upgrade the effect of quantitative required image feature at any time; That is with the image feature data of training again, the storage element (4) when loading tachysynthesis in the quantitative machine of test paper (6) is finished renewal.
Therefore, no matter the present invention all shows it totally different in the feature of known techniques with regard to purpose, means and effect, is a quantum jump of quantitative machine of rapid immunochromatographic test test paper and quantivative approach thereof, earnestly asks your juror and perceives, and granted patent is benefited society early.The foregoing description only has been to be convenient to explanation and the act row, and the patent right protection domain that the present invention advocated is as the criterion with claims institute restricted portion.
Claims (19)
1. quantitative machine of rapid immunochromatographic test test paper is characterized in that comprising:
One image acquisition unit has the optical image capture device in it, for the digitized video of acquisition rapid immunochromatographic test test paper;
One feature extraction unit captures at least one quantitative characteristic of the digitized video of this check test paper with digitized video handling procedure selectivity;
One neural network dosing unit has the cognitive time network of a plurality of plasticity, and the cognitive time network of each plasticity is to utilize the error back algorithm to come the principal character of quantitative testing rapid immunochromatographic test test paper;
One storage element stores the complex parameter value, and this plural number kind parameter value comprises critical value and system's related parameter values of a plurality of eigenwerts, the weighted value of the cognitive time network of a plurality of plasticity, the cognitive time network of a plurality of plasticity;
One output unit can calculate the neural network dosing unit quantitative result data delivery to output unit and show;
In view of the above, utilize the principal character of optical image technology acquisition rapid immunochromatographic test test paper, and with this principal character after the neural network dosing unit quantitative Analysis of core technology, obtain a certain amount of result, and be shown in output unit.
2. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1, it is characterized in that: the principal character of this rapid immunochromatographic test test paper comprises: Gong the quantitative Analysis rapid immunochromatographic test test paper result's of verification test product pipeline, verification test result line image feature.
3. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: the cognitive time network of this plasticity is to form through a plurality of weighted values and critical value foundation.
4. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this image acquisition unit is to adopt backlight type charge coupled cell, metal-oxide semiconductor as image capture unit.
5. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this image acquisition unit is to adopt optical scanning element as image capture unit.
6. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this feature extraction unit is to be responsible for carrying out by the microprocessor with high speed arithmetic logical operation characteristic handling and computing.
7. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this feature extraction unit is to be responsible for carrying out by digital signal processor handling and computing.
8. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this neural network dosing unit is to be responsible for carrying out by the microprocessor with high speed arithmetic logical operation characteristic handling and computing.
9. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: be to be responsible for carrying out by digital signal processor handling and computing.
10. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this output unit comprises a LCD, a plurality of light emitting diode and a loudspeaker.
11. the quantitative machine of rapid immunochromatographic test test paper as claimed in claim 1 is characterized in that: this feature extraction unit, neural network dosing unit and storage element can be integrated on the integrated circuit platform.
12. a rapid immunochromatographic test test paper quantivative approach comprises the following steps:
(A) provide the rapid immunochromatographic test test paper of a specified quantitative;
(B) this test paper is inserted an image acquisition unit, to obtain the digitized video of this test paper;
(C) digitized video to the test paper of obtained specified quantitative carries out preliminary interpretation, to judge whether this detection for the treatment of that quantitative test paper carries out is the PRELIMINARY RESULTS of Validity Test and test;
(D) utilize a feature extraction unit to capture at least one principal character in the digitized video of this test paper;
(E) see through a neural network dosing unit principal character of the digitized video that captured is carried out quantitative Analysis;
(F) will calculate the resulting quantitative data result in back and export output unit to.
13. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12 is characterized in that this optical image capture device is to utilize backlight type charge coupled cell or complementary metal oxide silicon to capture the digitized video of this test paper.
14. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12 is characterized in that: this optical image capture device is to utilize optical scanning element to capture the digitized video of this test paper.
15. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12 is characterized in that: whether the described preliminary interpretation of step (C) is as serving as the successful analysis foundation of test with verification test product pipeline and the reacted shade of verification test result line.
16. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12 is characterized in that: in the described step (D), described principal character is to comprise verification test product pipeline, verification test result line.
17. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12, it is characterized in that: in the described step (D), more utilize storage unit stores plural number to plant parameter value, this plural number kind parameter value comprises critical value and system's related parameter values of the weighted value of a plurality of eigenwerts, a plurality of neural networks, a plurality of neural networks.
18. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12, it is characterized in that: described neural network dosing unit is to set up a plasticity cognition network through a plurality of weighted values and critical value, and a plurality of eigenwerts are imported the cognitive time network of this plasticity, handle to carry out quantitative Analysis.
19. a kind of rapid immunochromatographic test test paper quantivative approach as claimed in claim 12, it is characterized in that: described output unit comprises a LCD, a plurality of light emitting diode and a loudspeaker.
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| CN 03154445 CN1603826A (en) | 2003-09-29 | 2003-09-29 | Rapid immune assay test paper batchmeter and quantitative method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105606608A (en) * | 2016-03-28 | 2016-05-25 | 广州博厚生物科技有限公司 | Image gray-scale processing based data computing method and application thereof in detection field |
| TWI603081B (en) * | 2015-05-04 | 2017-10-21 | 宏達國際電子股份有限公司 | System for physiologic parameter examination and method for test strip recognition and interpretation |
| CN107677812A (en) * | 2017-09-20 | 2018-02-09 | 福州大学 | A kind of golden immunochromatography detection method and device based on convolutional neural networks |
| CN110456050A (en) * | 2019-07-11 | 2019-11-15 | 台州云海医疗科技有限公司 | Portable intelligent digitizes helminth in-vitro diagnosis instrument |
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2003
- 2003-09-29 CN CN 03154445 patent/CN1603826A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI603081B (en) * | 2015-05-04 | 2017-10-21 | 宏達國際電子股份有限公司 | System for physiologic parameter examination and method for test strip recognition and interpretation |
| US9810639B2 (en) | 2015-05-04 | 2017-11-07 | Htc Corporation | System for physiologic parameter examination and method for test strip recognition and interpretation |
| CN105606608A (en) * | 2016-03-28 | 2016-05-25 | 广州博厚生物科技有限公司 | Image gray-scale processing based data computing method and application thereof in detection field |
| CN105606608B (en) * | 2016-03-28 | 2018-12-07 | 广州博厚医疗技术有限公司 | Based on image grayscale processing method for computing data and its in the application of detection field |
| CN107677812A (en) * | 2017-09-20 | 2018-02-09 | 福州大学 | A kind of golden immunochromatography detection method and device based on convolutional neural networks |
| CN110456050A (en) * | 2019-07-11 | 2019-11-15 | 台州云海医疗科技有限公司 | Portable intelligent digitizes helminth in-vitro diagnosis instrument |
| CN110456050B (en) * | 2019-07-11 | 2022-07-19 | 台州云海医疗科技有限公司 | Portable intelligent digital parasite in vitro diagnostic instrument |
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