CN1596642A - Bacteriostatic chemical-yijunmeisu and its application - Google Patents
Bacteriostatic chemical-yijunmeisu and its application Download PDFInfo
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- CN1596642A CN1596642A CN 200410051221 CN200410051221A CN1596642A CN 1596642 A CN1596642 A CN 1596642A CN 200410051221 CN200410051221 CN 200410051221 CN 200410051221 A CN200410051221 A CN 200410051221A CN 1596642 A CN1596642 A CN 1596642A
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Abstract
A bacteriostatic chemical 'Yijunmeisu' for preventing and eliminating mite, tick and other insects is disclosed. Its advantage is no environmental pollution.
Description
Technical field
The present invention relates to plant protection and biological pesticide technical field, specifically be meant antibacterial mycin and the application thereof of a kind of desinsection and inhibition plant pathogenic fungi.
Background technology
Antibiotic is one of important biopesticide, it is a kind of natural biological insecticide, has characteristics such as low to the person poultry toxicity, that degraded is rapid, does not have " three cause " effect, meet the requirement of crop pests such as non-environmental pollution control vegetables, thereby become one of focus of researching and developing in recent years both at home and abroad.Macrolide antibiotics is the alkalescent antibiotic of a class by the streptomycete generation, has 12～22 carbon lactone cyclisation structures.Medically, with erythromycin the cell nucleus glycoprotein body 50S subunit unit that the macrolide antibiotics of representative mainly acts on bacterium, it is synthetic to hinder bacterioprotein, belongs to the inhibitor in vegetative period.On agricultural, the macrolide antibiotics of representing with Avermectin has very high insecticidal activity to multiple parazoon and pest evil mite.But the macrolides compound that has insecticidal activity and restraining epiphyte activity does not simultaneously see that report is arranged.
Summary of the invention
Purpose of the present invention is exactly in order to solve above-mentioned the deficiencies in the prior art part, a kind of antibacterial mycin and application thereof that suppresses plant pathogenic fungi and insecticidal activity that have is provided, this antibacterial mycin is applicable to control insect and 11 plant species disease funguses etc., the insect of Lepidoptera (Lepidoptera) and diptera (Diptera) particularly, and free from environmental pollution, have the effect of attracting desinsection and inhibition fungal diseases of plants.
The present invention is achieved through the following technical solutions: described antibacterial mycin has following molecular formula:
Described antibacterial mycin has very strong insecticidal activity, be applicable to control mite, tick and other insect, comprise beet armyworm, tobacco budworm, cotton bollworm, imported cabbageworm, corn borer, diamond-back moth, leaf roller, cutworm, subtropics mythimna separata, rain moth section, India paddy phycitid, Pectinophora gossypiella; Also coleoptera and weevil there are control efficiency, comprise colorado potato beetle, three chrysomelid, Japanese fox rutelians of cucumber 11 asterophyllite first, cucumber, boll weevil; Also effective to the control Diptera pest, comprise housefly, Dulicidae, fruit bat, horn fly and Agromyzidae effective; Anoplura, Isoptera and Hymenoptera ant and mite also there are control efficiency, comprise citrus red mite, tetranychid; Simultaneously parazoon there is control efficiency, comprises water flea, Chinese anchor head lice.
Described antibacterial mycin be applicable to prevent and treat that the sick germ of sweet potato soft rot germ, citrus sour rot germ, peach scab germ, early blight of tomato germ, tomato bud branch, lettuce stalk break germ, downy mildew of lichee germ, anthrax-bacilus, curved spore are mould, the plant disease of Phomopsis and the rotten grape spore of fruit.Wherein the activity to Phomopsis is the strongest, concentration (IC in the inhibition
50Value) only is 0.1 μ g/ml.
In order to realize the present invention better, described antibacterial mycin is mixed with cream preparation when being applied to desinsection, and its weight percent proportioning is: antibacterial mycin 1～30%, emulsifier 5～20%, solvent 50～94%; Its best proportioning is: antibacterial mycin 5～10%, emulsifier 8～15%, solvent 75～87%, process for preparation is: antibacterial mycin and emulsifier (surfactant) are placed reactor, be to react 30～35 minutes under 300～320 rev/mins the condition at 28～35 ℃ of temperature, rotating speed, and then adding solvent, continue to stir 30～35 minutes, get final product after stirring.Wherein, emulsifier comprises farming breast 2201, agricultural newborn 0203B, YZ960; Solvent comprises isopropyl alcohol, acetone, dimethyl formamide.
By literature search, the spectral data of described antibacterial mycin is identical with A2 in antimycin (antimycins) series.Antimycin series is that Dunshee equals to get from the unidentified streptomycete Streptomyces of strain sp. in 1949 the earliest, because means of testing fell behind at that time, have to its molecular formula, according to its molecular weight as can be known his compound that obtains at that time be antimycin A3 (molecular weight 548).In 1988, Abidi etc. isolated eight Antimycin series matter antimycin A1～A8 (common name antimycin A) altogether by HPLC from the mixture that contains antimycin A3, and with the NMR technology their structure were confirmed.Biological activity test is found, antimycin A3 has very strong bactericidal activity, antibacterial mycin (antimycins) A2 also has very strong bacteriostatic activity, at concentration dilution to 1: 800,000,000 o'clock, it still had inhibitory action to the many spores of ball mould (Nigrospora sphaerica), but did not have as yet so far antibacterial mycin than the bacteriostatic activity research of system and the report of insecticidal activity.
The present invention adopts a kind of bacterial strain GAAS7310 of new streptomyces bacterial classification, it is separating obtained from adopting in the soil specimen of Xinjiang Tianshan, this bacterial strain is being positioned at Chinese typical culture collection center (CCTCC) preservation in Wuhan on July 7th, 2003, and deposit number is CCTCC M 203058Streptmyces sp.GAAS7310.
Described antibacterial mycin can make by following method:
The first step inoculates the GAAS7310 nutrient medium into the nutrition medium, and growth is 48 hours under the vibration of 28 ℃ and 250rpm; Get ripe phase I nutrition culture inoculating nutrient medium, obtain secondary nutrition culture, under 28 ℃ and 300rpm condition, cultivated 48 hours, the strain fermentation of maturation is cultivated, under 28 ℃ and 300rpm, keep cultivating 4 days.The fermentation product of available suitable solvent extraction maturation also obtains metabolite by chromatographic isolation;
The fermentation of second step finishes, mycelia is obtained in method separation with plate compression, the acetone soln of mycelia with 1: 2 leached 10 hours, with the concentrated acetone that goes of leaching liquid, use the ethyl acetate extraction working substance again, the acetic acid ethyl acetate extract concentrating under reduced pressure is obtained grease, the extraction concentrate is mixed silica gel and is crossed post, with ethyl acetate, benzinum silica gel column chromatography and concentrate repeatedly, obtain the pure product of oily mater.
The present invention compared with prior art has following advantage and beneficial effect:
1, compound of the present invention is the antibiotic of desinsection and control fungal diseases of plants, simple, easy to operate, the easy preparation of its preparation process thereof.
2, antibacterial mycin of the present invention is good to pest controling effects such as diamond-back moths, lacks than the conventional dose consumption, can overcome the pesticide resistance of insect such as diamond-back moth to conventional pesticide, can suppress 11 plant species disease funguses simultaneously.
3, antibacterial mycin of the present invention is safe in utilization, free from environmental pollution, is a kind of non-harmful biopesticide.
Embodiment
Below in conjunction with embodiment, the present invention is done detailed description further.
Embodiment one
The antibacterial mycin of the present invention is that the mycelium from Streptmyces sp.GAAS7310 extracts, detailed process is for to carry out fermented and cultured with bacterial strain Streptmyces sp.GAAS7310 at 500L on fermentation tank, zymotic fluid is with after mycelia separates, and mycelia is used the acetone lixiviate.After macroporous resin adsorption, water and eluent ethyl acetate behind eluent ethyl acetate liquid concentrating under reduced pressure, carry out silica gel column chromatography and separate successively with acetone extract.Adopt the biologically active tracking and testing, from the position of 30%EtOAc, get the antibacterial mycin of strong active component, white needle-like crystals, mp 140-142 ℃, [α]
D 20+ 114.7 (c 0.002, acetone).
13CNMR shows in the molecule has 7 quaternary carbons, 10 methines, 5 methylene and 5 methyl.In conjunction with molecular ion peak in the FABMS spectrum is 535[M+1]
+, determine that molecular formula is C
27H
38N
2O
9, degree of unsaturation U=10.
13Can see 5 carbonyls are arranged in the molecule [δ 175.7 (C), 174.1 (C), 170.9 (C) in the CNMR spectrogram, 170.3 (C), 160.7 (CH)], 6 olefinic carbon [δ 151.6 (C), 128.6 (C), 125.2 (CH), 122.6 (CH), 119.3 (CH), 114.5 (C)], (δ 76.3 for 3 company's oxygen methines, 74.8,72.2), (δ 54.7 for 3 methines, 51.1,41.8), (δ 36.7,29.7 for 5 methylene, 28.6,27.2,23.1) and 5 methyl (δ 18.2,15.4,2 * 17.0,11.9).Compound has 10 degrees of unsaturation, removes above-mentioned 5 carbonyls and 1 phenyl ring, and 1 ring also should be arranged in the compound.
1HNMR spectrum show exist in the molecule one even the trisubstituted benzene ring [δ 8.47 (and dd, 8,1.5Hz), 7.77 (dd, 8,1.5Hz) and 6.92 (dd, 8,8Hz)], three active hydrogens [δ 12.83 (s), 9.09 (s), 8.31 (d, 8Hz)], have 3350cm on the IR spectrum
-1There is the phenolic hydroxyl group with the carbonyl chelating in the peak in δ 12.83 (s) the prompting molecule.Because
1H-
1(d 1.5Hz) is correlated with, and DEPT shows δ with δ 8.52 to can be observed δ 9.09 (s) among the HCOSY
H8.52 be connected in δ
C160.7 on, therefore can infer that δ 9.09 (s) is NH, links to each other with formoxyl.Another one active hydrogen (δ 8.31) and δ 5.41 couplings illustrate that this active hydrogen also is NH, and it are relevant with carbonyl (δ 170.9), and pointing out it also is amide groups.Also remaining six oxygen are removed three carbonyls in the molecule,
13C NMR spectrum show have in the molecule three alkyl carbon directly link to each other with oxygen atom [δ 4.9 (m), 5.0 (m), 5.70 (dq, 7,7Hz)], illustrate that other three carbonyls are ester group.By two dimension
1H-
1HCOSY, HMQC and HMBC technology, the structure of deriving antibacterial mycin compound is as follows:
The inventor has the embodiment that much successfully prepares preparation through long-term test, research, shown in being listed in the table below for example below:
Table 1
| 39% | |||
| Preparation | Bacteriostatic chemical-yijunmeisu and emulsifying agent are placed reactor, are reaction 35 minutes under 320 rev/mins the condition at the rotating speed of 35 ℃ of reactors of temperature, and then add selected organic solvent and continue to stir and got final product in 35 minutes. | Bacteriostatic chemical-yijunmeisu and emulsifying agent are placed reactor, are reaction 30 minutes under 300 rev/mins the condition at the rotating speed of 30 ℃ of reactors of temperature, and then add selected organic solvent and continue to stir and got final product in 30 minutes. | Bacteriostatic chemical-yijunmeisu and emulsifying agent are placed reactor, are reaction 32 minutes under 310 rev/mins the condition at the rotating speed of 28 ℃ of reactors of temperature, and then add selected organic solvent and continue to stir and got final product in 32 minutes. |
| Effect | Working concentration is higher, large usage quantity, and the kill pests effect is better. | Working concentration is lower, and consumption is less, and the kill pests effect is fine. | Working concentration is very low, consumption seldom, the kill pests effect is fine |
Table 2
| Go into selected organic solvent continuation stirring got final product in 35 minutes. | Select for use organic solvent to continue to stir got final product in 30 minutes. | Select for use organic solvent to continue to stir got final product in 32 minutes. | |
| Effect | Working concentration is higher, large usage quantity, and the kill pests effect is better. | Working concentration is lower, and consumption is less, and the kill pests effect is fine. | Working concentration is very low, consumption seldom, the kill pests effect is fine |
Embodiment eight
Antibacterial mycin of the present invention is applicable to control insect, mite and tick, and insect, mite and tick amount of suppression are used in the place that is included in insect, mite and tick.Such environment is represented in the place of insect, mite and tick: wherein, the larva of insect, mite and tick, adult and ovum comprise air, food and the object that is contacted.For example, phytophagous insect or mite can be controlled by described reactive compound being adapted to insect and mite food or parasitic plant part (particularly leaf).Relevant with access times and using method, relevant with the kind of the insect that is prevented and treated simultaneously by antibacterial mould effect of usually preventing and treating insect of the present invention.
A variety of Chinese cabbage is potted plant, and when growing to 5 leaves, every basin inserts 50～60 of low age resistance diamondback moth larvaes, waits to try the movable stable back spray of worm preparation of the present invention, handles dead borer population amount, the dead state instituted an inquiry two days later.4 repetitions are established in each processing, calculate lethality, use the result as shown in the table.
The control efficiency of the antibacterial mycin antagonism of table 3 diamond-back moth
| Medicament and concentration | For examination borer population (head/basin) | Handle lethality after 2 days | |
| Preparation of the present invention | 75ppm | ????52 | ????97.62％ |
| 30ppm | ????49 | ????88.64％ | |
| 15ppm | ????50 | ????78.23％ | |
Embodiment nine
As object of reference, detect the insecticidal effect of preparation of the present invention with the water spider of natural propagation growth in the pond.Get the pond water that contains the water spider, add the preparation of the present invention and the crystal chlorophos of variable concentrations respectively, establish the control group that does not add medicine simultaneously, observed continuously 3 days, statistical computation the present invention is to the killing effect of water spider.The use result is as shown in the table.
The antibacterial mycin of table 4 is to the killing effect of water spider
| Medicine | Concentration (ppm) | Killing effect (%) | ||
| ??24hr | ??48hr | ??72hr | ||
| Preparation of the present invention | ??0.021 | ??89 | ??100 | ??100 |
| ??0.013 | ????83 | ????90 | ????93 | |
| ??0.004 | ????63 | ????77 | ????83 | |
| Chlorophos | ??0.5 | ????88 | ????90 | ????93 |
Antibacterial mycin also has very strong knockdown effect to housefly, is just benumbed in 3h after food is mixed with the white sugar of antibacterial mycin getting for the examination housefly, can not normal flight, at the bottom of lying on the back bottle, some stands up back attempt flight, but unable flying up, six foot performances are unable, can not support polypide, it is motionless substantially then to try worm, enters the stupor stage, idol has wing to quiver, touch gently with writing brush, the foot of examination worm is just shaken, and is that the in vitro dead polypide of 50 and 25 μ g/ml has surpassed 90% in 24h post processing concentration.The observed data of getting behind the 24h is carried out data analysis, and calculating antibacterial mycin is Y=4.7695+1.1884X to the virulence regression equation of housefly, r=0.9866, LC
50Value is 1.56 μ g/ml, and the result shows that antibacterial mycin has very high cytotoxicity to adult housefly, can be used for preventing and treating sanitary insect pest.
Embodiment ten
Germ suppresses experimental technique, and the concentration that is made into antibacterial mycin is the band poison medium of 100 μ g/ml.With sterile water in contrast.Each component is done 12 processing, and 5 repetitions are established in each processing, 5 contrasts.In 28 ℃ of cultivations, measured hyphal diameter in per 12 hours, the cross colony diameter of measuring is got its mean value and is deducted primordial hypha piece diameter, one week of METHOD FOR CONTINUOUS DETERMINATION.It is inhibited to the mycelial growth of which kind pathogen respectively to observe these two kinds of active components.
Set each component to each concentration of treatment gradient for the examination bacterium on the basis of qualitative analysis, inhibited pathogen is carried out quantitative analysis, data processing method is:
Test is in culturing room's (temperature: 28 ℃; Relative moisture 70%～80%) carry out in, obtain regression equation and IC after the investigation result
50Value.
Qualitative test result shows that antibacterial mycin has the obvious suppression effect to the mycelial growth of, Phomopsis mould for the sick germ of sweet potato soft rot germ, citrus sour rot germ, peach scab germ, early blight of tomato germ, tomato bud branch, lettuce stalk break germ, downy mildew of lichee germ, anthrax-bacilus, the curved spore of examination in the disease fungus and the rotten grape spore of fruit when concentration is 100 μ g/ml.
On this basis each disease fungus is carried out inhibition test, the result is as shown in table 5.Result of the test shows that the antimicrobial spectrum of antibacterial mycin is wider, and the common fungal disease pathogen of field fruits and vegetables is all had good inhibitory effect, suppress active the highest relatively be Phomopsis, IC
50Value only is 0.1 μ g/ml, IC
50Value has 7 kinds of bacterium such as anthracnose germ, downy mildew of lichee germ, an IC below 100 μ g/ml
50Value has 2 kinds of bacterium of the sick germ of sweet potato soft rot germ and tomato bud branch between 100 μ g/ml～200 μ g/ml, active the most weak relatively be rotten grape spore really, IC
50Value is 398.07 μ g/ml.Each is handled except diameter is significantly less than contrast, and the density of mycelia also is significantly less than contrast.
The antibacterial mycin of table 5 is to the toxicity test of 11 kinds of disease funguses
IC
50Value (μ g/ml) regression equation correlation coefficient
Sweet potato soft rot germ 108.36 Y=2.5805+1.1890X 0.9755
Citrus sour rot germ 90.52 Y=3.9646+0.5291X 0.9412
Peach scab germ 96.16 Y=3.8957+0.5569X 0.9198
Early blight of tomato germ 70.34 Y=4.1612+0.4541X 0.9829
Sick germ 137.55 Y=3.1668+0.8572X 0.9728 of tomato bud branch
Lettuce stalk break germ 82.59 Y=2.3926+1.3602X 0.9818
Downy mildew of lichee germ 35.33 Y=3.1277+1.2094X 0.9802
Rotten grape spore 398.07 Y=2.8469+0.8281X 0.9967 of fruit
Anthracnose germ 23.34 Y=4.3846+0.4498X 0.9930
Curved mould 68.57 Y=3.9696+0.5612X 0.9916 of spore
Phomopsis 0.1 Y=5.2352+0.2316X 0.9740
Find out that from result of the test the present invention is fine to the control efficiency of insect and fungal diseases of plants, environment is not polluted.Can overcome the pesticide resistance of damage by disease and insect to medicament.As the control agent of damage by disease and insect, will help the stable yields and the high yield of agricultural, thereby produce bigger economic and social benefit.
As mentioned above, can realize the present invention preferably.
Claims (7)
1, antibacterial mycin is characterized in that, it has following molecular formula:
2, the application of the described antibacterial mycin of claim 1, it is characterized in that, it is applicable to control mite, tick and other insect, comprises beet armyworm, tobacco budworm, cotton bollworm, imported cabbageworm, corn borer, diamond-back moth, leaf roller, cutworm, subtropics mythimna separata, rain moth section, India paddy phycitid, Pectinophora gossypiella; Be applicable to control coleoptera and weevil, comprise colorado potato beetle, three chrysomelid, Japanese fox rutelians of cucumber 11 asterophyllite first, cucumber, boll weevil; Be applicable to the control Diptera pest, comprise housefly, Dulicidae, fruit bat, horn fly and Agromyzidae effective; Be applicable to and prevent and treat Anoplura, Isoptera and Hymenoptera ant and mite, comprise citrus red mite, tetranychid; Be applicable to controlling animal parasites, comprise water flea, Chinese anchor head lice; Be applicable to controlling plant diseases.
3, the application of antibacterial mycin according to claim 2, it is characterized in that, described be applicable to controlling plant diseases comprise be applicable to prevent and treat that the sick germ of sweet potato soft rot germ, citrus sour rot germ, peach scab germ, early blight of tomato germ, tomato bud branch, lettuce stalk break germ, downy mildew of lichee germ, anthrax-bacilus, curved spore are mould, the plant disease of Phomopsis and the rotten grape spore of fruit.
4, the application of antibacterial mycin according to claim 2 is characterized in that, is mixed with cream preparation when being applied to desinsection, and its weight percent proportioning is: antibacterial mycin 1～30%, emulsifier 5～20%, solvent 50～94%; Process for preparation is: antibacterial mycin and emulsifier are placed reactor, are to react 30～35 minutes under 300～320 rev/mins the condition at 28～35 ℃ of temperature, rotating speed, and then add solvent, continue to stir 30～35 minutes, get final product after stirring.
5, the application of antibacterial mycin according to claim 4 is characterized in that, the optimum weight per distribution ratio of its application is: antibacterial mycin 5～10%, emulsifier 8～15%, solvent 75～87%.
6, the application of antibacterial mycin according to claim 4 is characterized in that, described emulsifier comprises farming breast 2201, agricultural newborn 0203B, YZ960.
7, the application of antibacterial mycin according to claim 4 is characterized in that, described solvent comprises isopropyl alcohol, acetone, dimethyl formamide.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174440A (en) * | 2011-01-28 | 2011-09-07 | 广东省农业科学院植物保护研究所 | Streptomyces antibioticu and biological preparation prepared from active product thereof and application |
| CN114957198A (en) * | 2022-03-29 | 2022-08-30 | 宁波大学 | Antimycin compound and preparation method and application thereof |
-
2004
- 2004-08-26 CN CN 200410051221 patent/CN1265704C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174440A (en) * | 2011-01-28 | 2011-09-07 | 广东省农业科学院植物保护研究所 | Streptomyces antibioticu and biological preparation prepared from active product thereof and application |
| CN102174440B (en) * | 2011-01-28 | 2013-01-16 | 广东省农业科学院植物保护研究所 | Streptomyces antibioticu and biological preparation prepared from active product thereof and application |
| CN114957198A (en) * | 2022-03-29 | 2022-08-30 | 宁波大学 | Antimycin compound and preparation method and application thereof |
| WO2023184724A1 (en) * | 2022-03-29 | 2023-10-05 | 宁波大学 | Antimycin compound as well as preparation method therefor and use thereof |
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