CN1586296A - Functional food capable of removing hydroxy free radical in body and its preparing method - Google Patents

Abstract

The present invention discloses a kind of functional food capable of removing free hydroxy radical in body. The functional food consists of rhodiola root in 9-16.7 wt% and red yeast distiller's grains in 83.3-91 wt%. The preparation process includes crushing rhodiola root to fine powder of 120 mesh, soaking the fine powder with 75 % concentration alcohol solution for 12 hr, filtering, distilling filtrate at 70 deg.c and 0.007 MPa to eliminate alcohol, drying, extruding, red yeast distiller's grains, drying at 80 deg.c and crushing to fine powder of 120 mesh, and mixing the fine red yeast distiller's grains powder with fine rhodiola root powder. The functional food has excellent function of removing free hydroxy radical in body and can strengthen physique and delay senility.

Description

Can remove functional food of hydroxy radical in the body and preparation method thereof

Technical field:

The present invention relates to a kind of functional food, and the method for this functional food of preparation.

Background technology

In causing the various free radicals of body oxidative damage, hydroxy radical (OH) is maximum to body harm, and hydroxy radical (OH) almost can react with all cell components (nucleic acid, protein and lipid etc.), causes body injury, causes disease and aging.The development of therefore removing hydroxy radical functional food in the body is significant, and does not also have this series products in the market.

Summary of the invention

At above-mentioned deficiency, the invention provides a kind of can health invigorating, the functional food that can remove hydroxy radical in the body of slow down aging and preparation method thereof.

For achieving the above object the present invention by the following technical solutions: the present invention can remove the functional food of hydroxy radical in the body, is made up of the Radix Rhodiolae of 16.7-9% (weight) and the Monas cuspurpureus Went distiller grains of 83.3-91% (weight).

Can remove the preparation method of the functional food of hydroxy radical in the body:

1) get root of Rhodiola sachalinensis drying and crushing to 120 order fine powder,

2) also constantly stirred described Radix Rhodiolae fine powder 12 hours with 75% soak with ethanol, the ethanol volume is 5 times of Radix Rhodiolae volume,

3) described Radix Rhodiolae and alcoholic acid mixed solution are filtered,

4) ethanol is removed in the distillation under 70 ℃, 0.007Mpa of this filtrate, and is dry then,

5) get the Monas cuspurpureus Went distiller grains extruding, dry under 80 ℃, be crushed to 120 order fine powders,

6), even by 5: 1 mixed with step 4 gained Radix Rhodiolae fine powder with step 5 gained Monas cuspurpureus Went distiller grains fine powder.

The mixed powder of step 6 gained is incapsulated or be pressed into tablet, and 7KGy is adopted in the encapsulation back ⁶⁰The Co irradiation sterilization.

Monas cuspurpureus Went is that the monascus fungal inoculum forms in the rice top fermentation that cooks, and its red complexion is red, and it is synthetic that Monas cuspurpureus Went has the body of inhibition inner cholesterol, the effect of blood fat reducing, and simultaneously also to hypertension, diabetics has certain auxiliary treatment effect.

Radix Rhodiolae is Crassulaceae (Crassulaceae) rhodiola (Rhodiola.) perennial herb or semishrub plant.There is kind more than 90 in this genus whole world, is distributed in East Asia, the Central Asia, Siberia and north America region.About 73 kinds of China occupies first of the world, mainly is distributed in ground such as northeast, Tibet, Xinjiang.There is long history in China in the application facet of Radix Rhodiolae: be recorded in Tibetan medicine's Four-Volume Medical Code in 1200 Christian eras the earliest, Radix Rhodiolae be designated as " cool in nature, heat clearing away, nourishing vigour ".1596 Christian eras, in Li Shizhen (1518-1593 A.D.) Compendium of Material Medica grass portion the 20 volume Radix Rhodiolae is classified as top gradely, claim it that effect of strengthening the body resistance, benefiting qi and nourishing blood, clearing heat and moistening lung is arranged.China's Ministry of Public Health approval in 1991 Radix Rhodiolae is the functional food resource.The chemical constituent of Radix Rhodiolae is very complicated, except that containing aminoacid, trace element and multivitamin, has isolated the material of multiple biologically actives such as flavonoid, glycoside at present.Nearly ten years studies have shown that, Radix Rhodiolae have functions such as resisting fatigue, anti-hypoxia, radioprotective.

The present invention has the function of good removing hydroxy radical.Experiment can prove in following external and body.

Experiment in vitro

External salicylic acid is caught experiment

Hydroxy radical (OH) by Fenton reaction generation, with salicylic acid generation oxidation reaction, generation has 2 of characteristic absorption to 510 nanometer light, and the 3-resorcylic acid is caught the clearance rate that the resulting product of hydroxy radical (OH) is determined hydroxy radical (OH) by measuring salicylic acid.

Contain 0.15mmol/L FeSO in the 3ml reactant liquor ₄, 6mmol/L H ₂O ₂And functional food (hereinafter to be referred as the being tried thing) solution that can remove hydroxy radical in the body of variable concentrations.Measure the light absorption value at 510nm place after 1 hour 37 ℃ of reactions.Light absorption value is low more, and the effect of removing hydroxy radical (OH) is good more.

The computational methods of hydroxy radical (OH) clearance rate: [1-(A _i-A _Io)/A _o] * 100% wherein, A _iTried the light absorption value of thing, A for containing certain concentration _IoTried the background values of thing solution when not adding developer, A _oFor not adding the contrast solution light absorption value that is tried thing.Experimental result is as follows:

The external salicylic acid of table 1 is caught experiment and is detected and to be tried thing to the scavenging action of OH (n=10 x ± s)

Sample concentration (μ A _532nmClearance rate (%)

Contrast 0 0.211 ± 0.014-

Tried thing 10 0.197 ± 0.015 5.2

a

Tried thing 20 0.183 ± 0.012 11.8

b

Tried thing 40 0.171 ± 0.013 17.5

b

Tried thing 100 0.136 ± 0.011 33.1

A compares p＜0.05 with matched group, b compares p＜0.01 with matched group

As shown in Table 1, catch in the experiment at external salicylic acid, being tried thing all has scavenging action to hydroxy radical (OH) in experimental concentration (20～100 μ g/ml) scope.Become certain linear relationship between concentration and the clearance rate, increase gradually with the increase clearance rate of concentration.

The Phen-Cu-Vc-H2O2-DNA experiment

Coffee sieve quinoline (Phen) and metal Cu ²⁺Generate coordination compound (Phen) ₂Cu ²⁺, be reduced into by Vit.C again (Phen) ₂Cu ⁺, with H ₂O ₂Reaction generates hydroxy radical (OH), and the hydroxy radical of generation (OH) acts on DNA, produces the dna segment of 3 ' and 5 ' phosplate end, follows the oxidative degradation of DNA to produce chemiluminescence.

After adding is tried thing, if being tried thing has removing hydroxy radical (OH) function, then can make luminous intensity weaken (minimizing of light quantity subnumber), this variations in light is detected and record with the Weak-luminescence measuring instrument.The result is as follows:

Table 2 variable concentrations is tried thing to the influence of Phen-Cu-Vc-H2O2-DNA luminescence system (n=10 x ± s)

Sample is tried substrate concentration (μ g/ml) light quantity subnumber

Contrast 0 12540 ± 420

a

Tried thing 20 10300 ± 450

a

Tried thing 50 7440 ± 380

a

Tried thing 100 5570 ± 430

a

Tried thing 200 4650 ± 390

A compares p＜0.01 with matched group

From table 2, can see, along with the increase that is tried substrate concentration in the system, the light quantity subnumber that DNA is subjected to hydroxy radical to attack the damage product reduces gradually, when being tried substrate concentration and be 20 μ g/ml just with do not add the difference (p＜0.01) that the matched group that is tried thing produces highly significant, experiment shows and is tried the function that thing has good removing hydroxy radical.

Experiment in the body

14 monthly age of Kunming kind male mice is divided into 3 groups at random, is called that blank group, low dosage are tried the thing group, high dose is tried the thing group, 10 every group, the mice body weight does not have significant difference through between each group of t check.Adopt administration by gavage to be tried thing, irritate stomach every day once, irritate the stomach amount: high dose is tried thing group concentration 5g/100ml aqueous solution; Low dosage is tried thing group concentration 2.5g/100ml aqueous solution, and irritating body of stomach long-pending is the 0.2ml/10g body weight.The blank group is irritated the distilled water of stomach with volume.The continuous irrigation stomach is measured every biochemical index after 50 days.

Tried the influence of thing to hydroxy radical content in the 14 monthly age murine liver tissue

Method with D.G.Hafeman is measured hydroxy radical content in the murine liver tissue, the results are shown in Table 3.

Table 3. is tried thing to the influence of hydroxy radical content in the 14 monthly age murine liver tissue (n=10x ± s)

Group	2,5-DHBA * 100/ salicylic acid
		Contrast	????10.5±1.0
Low dosage is tried thing	????9.6±1.1*
		High dose is tried thing	????9.0±1.2*

* compare P＜0.05 with matched group

As can be seen from Table 3 14 the monthly age mice take and tried thing after 50 days, hydroxy radical content all significantly is lower than the blank group in high dose group, the low dose group hepatic tissue.Show and tried the function that thing has hydroxy radical in the direct removing body.

Tried the influence of thing to 14 monthly age mice serum malonaldehyde (MDA) content

Measure MDA content in the mice serum with thiobarbituricacid (TBA) method, the results are shown in Table 4.

Table 4 is tried the influence of thing to 14 monthly age mice serum MDA content

Group	MDA (nmol/ml serum)
		Contrast	6.00±0.44
Low dosage is tried thing	5.38±0.43*
		High dose is tried thing	5.19±0.45**

* compare P＜0.05 with matched group, * * and matched group be P＜0.01 relatively

As can be seen from Table 4 14 the monthly age mice take and tried thing after 50 days, high dose group, low dose group Content of MDA all significantly are lower than the blank group.

MDA (malondiadehycle) is the snperoxiaized a kind of end-product of cell membrane lipid, measures its content and can the indirect Estimation free radical attacks the degree that lipid in the biomembrane causes damage.The result of table 4 has shown that being tried thing has the removing free radical, reduces the effect that MDA generates in the serum.

Tried the influence of thing to content of protein carbonyl group in the 14 monthly age murine liver tissue

Method with C.N.Oliver is measured content of protein carbonyl group in the aged mouse hepatic tissue, the results are shown in Table 5.

Table 5. is tried the influence of thing to 14 monthly age murine liver tissue content of protein carbonyl group

Group	Carbonyl content (nmol/mg albumen)
		Contrast	1.18±0.19
Low dosage is tried thing	0.83±0.20*
		High dose is tried thing	0.77±0.15**

* compare P＜0.05 with matched group, * * and matched group be P＜0.01 relatively

The generation of carbonyl is the vital signs that protein molecule is modified by free-radical oxidation, because this feature has universality, thereby can judge by measuring carbonyl content whether protein is subjected to the oxidative damage of free radical.As can be seen from Table 5,14 the monthly age mice take tried thing after 50 days the hepatic tissue content of protein carbonyl group significantly be lower than matched group, illustrate that being tried thing has the certain protection effect to protein, also side light is tried thing and is had the function of removing free radical.

Tried thing by experiment confirm in the above external and body and had good removing hydroxy radical function.Because Monas cuspurpureus Went distiller grains does not belong to medicine, and Radix Rhodiolae is the health food function raw material of Ministry of Public Health approval.Therefore this functional food edible safety can be taken for a long time.

Specific embodiment

Can remove the functional food of hydroxy radical in the body is made up of the Radix Rhodiolae of 16.7-9% (weight) and the Monas cuspurpureus Went distiller grains of 83.3-91% (weight).

Can remove the preparation method of the functional food of hydroxy radical in the body:

1) get root of Rhodiola sachalinensis drying and crushing to 120 order fine powder,

2) also constantly stirred described Radix Rhodiolae fine powder 12 hours with 75% soak with ethanol, the ethanol volume is 5 times of Radix Rhodiolae volume,

3) described Radix Rhodiolae and alcoholic acid mixed solution are filtered,

4) ethanol is removed in the distillation under 70 ℃, 0.007Mpa of this filtrate, and is dry then,

5) get the Monas cuspurpureus Went distiller grains extruding, dry under 80 ℃, be crushed to 120 order fine powders,

6), even by 5: 1 mixed with step 4 gained Radix Rhodiolae fine powder with step 5 gained Monas cuspurpureus Went distiller grains fine powder.

The mixed powder of step 6 gained is incapsulated or be pressed into tablet, and 7KGy is adopted in the encapsulation back ⁶⁰The Co irradiation sterilization.

Claims (3)

1, a kind of functional food that can remove hydroxy radical in the body is made up of the Radix Rhodiolae of 16.7-9% (weight) and the Monas cuspurpureus Went distiller grains of 83.3-91% (weight).

2, the described preparation method that can remove the functional food of hydroxy radical in the body of claim 1: 1) get root of Rhodiola sachalinensis drying and crushing to 120 order fine powder,

2) stirred 12 hours with 75% soak with ethanol, the ethanol volume is 5 times of Radix Rhodiolae volume,

3) described Radix Rhodiolae and alcoholic acid mixed solution are filtered,

4) ethanol is removed in the distillation under 70 ℃, 0.007Mpa of this filtrate, and is dry then,

5) get the Monas cuspurpureus Went distiller grains extruding, dry under 80 ℃, be crushed to 120 order fine powders,

6), even by 5: 1 mixed with step 4 gained Radix Rhodiolae fine powder with step 5 gained Monas cuspurpureus Went distiller grains fine powder.

3, the preparation method that can remove the functional food of hydroxy radical in the body according to claim 2 is characterized in that: the mixed powder of step 6 gained is incapsulated or be pressed into tablet, and 7K Gy is adopted in the encapsulation back ⁶⁰The Co irradiation sterilization.