CN1861104A - Inula flower extractive used to breat diabets mellitus and hyperlipidemia - Google Patents
Inula flower extractive used to breat diabets mellitus and hyperlipidemia Download PDFInfo
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Abstract
A liquid extract of Inula japinice and/or Inula britannica for treating diabetes and hyperlipemia, decreasing blood sugar and blood fat and improving the tolerance to glucose is disclosed. It is extracted in the water contained solvent.
Description
Technical field
The present invention relates to Flos Inulae (covering) flower extract.Especially, the present invention relates to extract the purposes that Flos Inulae (covering) flower (comprising Inula japinica and Inula britannica) water extract, its preparation method, the compositions that contains this water extract and water extract that vegetable material obtains and compositions are used to prevent and treat diabetes and hyperlipemia with aqueous solvent.
Background technology
Flos Inulae (covering) flower (comprising Inula japinica and Inula britannica) belongs to feverfew.Effect with expectorant, the therapeutic method to keep the adverse QI flowing downwards, softening the hard mass and row water.Bibliographical information Flos Inulae (covering) flower aerial parts contains multiple compositions (Huang Taikang, conventional Chinese medicine composition and pharmacology handbook, p1608,1994) such as Flos Inulae (covering) flower lactone, gaillardin, Quercetin, quercimeritrin, caffeic acid, chlorogenic acid.
Relevant Inula platymiscium hypoglycemic activity bibliographical information:
1. about 70 kind of plant of Spain (comprising the Folium Salicis Babylonicae Flos Inulae, Inula salicina) are used for tea-drinking, infer to have hypoglycemic activity (Pardo de S., J Ethnopharmacol, 2005,98 (1-2): 1-19);
2. the total shape Radix Aucklandiae (Inula racemosa) root alcohol extract can reduce hormonal blood glucose in diabetic mice (Gholap S., Pharmazie, 2004,59 (11): 876-8);
3. total shape Radix Aucklandiae improves hormonal blood glucose in diabetic mice (Gholap S., Pharmazie, 2003,58 (6): 413-5) by regulating thyroxin;
4. total shape Radix Aucklandiae has the effect of the insulin sensitivity of raising (Kelly GS, Altern Med Rev, 2000,5 (2): 109-32) as health product;
5. total shape Radix Aucklandiae second ethanol extract can reduce blood glucose in diabetic mice, increases hepatic glycogen synthetic (Tripathi YB, 1995,33 (9): 686-9).
6. Flos Inulae (Inula britannica) water extract can be reconciled diabetic mice cellular immune function (Kobayashi T., Phytother Res, 2002,16 (4): 377-82);
7. " Rhizoma Cyperi Flos Inulae soup " adds treatment type ii diabetes one example report (Xiuyun Zhang, Shandong journal of Chinese medicine, 1996,15 (6): 255), flavor side's (12 flavor Chinese medicine).
The present invention adopts water and contains two kinds of Flos Inulae of extraction with aqueous solution (covering) flowering plant (comprising Inula japinica and Inula britannica) aerial parts acquisition extract of organic solvent.These two kinds of extracts have strong blood sugar lowering and effect for reducing blood fat, can significantly improve the alloxan diabetes mice to the glucose tolerance.About the research of Flos Inulae (comprising Inula japinica and Inulabritannica) extract for treating diabetic and hyperlipemia aspect after retrieval, not seeing with this research has identical report, and this research belongs to finds that first this plant has blood sugar lowering and hypolipidemic activity.
Summary of the invention
The present invention is embodied in the paragraph of following numbering:
1. a method for preparing the water extract of Flos Inulae comprises: obtain the Flos Inulae vegetable material; Extract the Flos Inulae vegetable material with aqueous solvent, obtain the water extract of Flos Inulae.
2. the method for paragraph 1, wherein extract the Flos Inulae vegetable material with aqueous solvent and comprise: (1) soaks the Flos Inulae vegetable material with aqueous solvent under proper condition; (2) separate liquid phase and solid phase; (3) randomly, repeating step (1) and (2) one or many (for example repeat 1-for several times, 1-5 time, 1-2 time, 2-5 time, 3 times, 4 times); (4) randomly, merge liquid phase; (5) randomly, will concentrate by the liquid phase that step (2) or step (4) obtain.
3. the method for paragraph 2, wherein felicity condition comprises described in the step (1):
TemperatureBe approximately 0-100 ℃, for example about 0-80 ℃, preferably approximately 0-60 ℃, for example about 4-50 ℃ as about 15-40 ℃, approximately 20-30 ℃, is about room temperature easily, also can be about 60-100 ℃;
PressureBe about 0.1 atmospheric pressure-5 atmospheric pressure, for example about 0.5 atmospheric pressure-3 atmospheric pressure, for example about 1 atmospheric pressure is about normal pressure easily; Soak
TimeBe about at least 1 hour, grow to for example 10 days, for example about 6 hours-5 days, approximately 12-96 hour, for example about 24,48,72 hours, perhaps any time wherein, preferably be not shorter than 24 hours;
The w/v of Flos Inulae vegetable material and aqueous solventBe about 1: 1 to about 1: 100 (kg/liter), for example about 1: 1 to about 1: 50, about 1: 2 to about 1: 40, preferably approximately 1: 5 to about 1: 20, more preferably about 1: 5 to about 1: 15 (kg/liter); Preferably when soaking, stir.
4. each method of paragraph 2-3, wherein separate in the step (2) by filter, centrifugal, natural subsidence waits and realizes.
5. each method of paragraph 2-4, wherein the concentrated of step (5) is concentrating under reduced pressure, and temperature can preferably be no more than about 60 ℃ at about 0 ℃ to about 80 ℃, and for example room temperature is to about 60 ℃, and for example approximately 30-60 ℃, as about 55 ℃; Concentrating of step (5) can be lyophilization.
6. each method of paragraph 1-5, wherein said Flos Inulae is Inula japinica or Inula britannica.
7. each method of paragraph 1-6, wherein said Flos Inulae vegetable material is the aerial parts of Flos Inulae plant, for example inflorescence.
8. each method of paragraph 1-7, wherein said aqueous solvent is water or the aqueous solution that contains organic solvent; Wherein organic solvent can be methanol, ethanol, propanol, isopropyl alcohol, acetone or n-butyl alcohol or their any combination.
9. each method of paragraph 1-7 wherein can be used organic solvent extraction in advance before extracting the Flos Inulae vegetable material with aqueous solvent.Organic solvent includes but not limited to: petroleum ether, chloroform, ethyl acetate, ether, methanol, ethanol, propanol, isopropyl alcohol, acetone or n-butyl alcohol or their any combination etc.
10. the water extract of Flos Inulae.
11. the water extract of paragraph 10, it is to obtain by any one method of paragraph 1-9.
12. the water extract of the Flos Inulae that can obtain by any one method of paragraph 1-9.
13. a compositions, it contains any one Flos Inulae water extract of paragraph 10-12.
14. the compositions of paragraph 13, it also contains excipient or carrier.
15. the compositions of paragraph 13 or 14, it is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
16. any one the Flos Inulae water extract or the compositions of paragraph 13 or 14 of paragraph 10-12 is used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improves the purposes of diabetics to the medicine of the tolerance of glucose in preparation.
Can be used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve the purposes of diabetics 17. Flos Inulae vegetable material or its extract (preferred water extract) are used for producing the compositions of the tolerance of glucose.
18. the purposes of paragraph 16, wherein said compositions are pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
19. blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve the method for diabetics to the tolerance of glucose comprise to the patient giving Flos Inulae vegetable material or its extract (preferred water extract) or containing the compositions of this extract or other product after by the processing of Flos Inulae vegetable material.
These aspects of the present invention and others will be further elucidated hereinbelow, but they are not replied and are interpreted as and limit the scope of the present invention.
Description of drawings
No accompanying drawing.
The specific embodiment
Purpose of the present invention is the water extract that obtains Flos Inulae (covering) flowering plant (comprising Inula japinica and Inula britannica) water extract and contain organic solvent, these extracts can be used for treating the purposes of diabetes and hyperlipidemia, are particularly useful for treating diabetes.
Therefore, first aspect the present invention relates to a kind of method for preparing the water extract (perhaps extractum) of Flos Inulae, comprising: obtain the Flos Inulae vegetable material; Extract (perhaps leaching) Flos Inulae vegetable material with aqueous solvent, obtain the water extract of Flos Inulae.Method of the present invention is used for can be used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improving the active component of diabetics to the tolerance of glucose from the extraction of Flos Inulae (covering) flowering plant material.
Can pass through decocting method, infusion process, percolation or steam distillation etc. and extract (perhaps leaching) Flos Inulae vegetable material.
In a specific embodiments, extract the Flos Inulae vegetable material with aqueous solvent and comprise: (1) soaks the Flos Inulae vegetable material with aqueous solvent under proper condition; (2) separate liquid phase and solid phase; (3) randomly, repeating step (1) and (2) one or many (for example repeat 1-for several times, 1-5 time, 1-2 time, 2-5 time, 3 times, 4 times); (4) randomly, merge liquid phase; (5) randomly, will concentrate by the liquid phase that step (2) or step (4) obtain.
In step (1), described felicity condition can be determined by test.Temperature is typically about 0-100 ℃, and for example about 0-80 ℃, preferably approximately 0-60 ℃, for example about 4-50 ℃ as about 15-40 ℃, approximately 20-30 ℃, is about room temperature easily, also can be about 60-100 ℃.Pressure is typically about 0.1 atmospheric pressure-5 atmospheric pressure, for example about 0.5 atmospheric pressure-3 atmospheric pressure, and for example about 1 atmospheric pressure is about normal pressure easily.Normally about at least 1 hour of soak time is grown to for example 10 days, for example about 6 hours-5 days, approximately 12-96 hour, for example about 24,48,72 hours, perhaps any time wherein, preferably is not shorter than 24 hours.The w/v of Flos Inulae vegetable material and aqueous solvent is typically about 1: 1 to about 1: 100 (kg/liter), for example about 1: 1 to about 1: 50, about 1: 2 to about 1: 40, preferably approximately 1: 5 to about 1: 20, more preferably about 1: 5 to about 1: 15 (kg/liter).Preferably when soaking, stir, for example repeatedly stir or continuous stirring.
Separation in the step (2) can realize by any method known in the art, includes but not limited to, filters, centrifugal, natural subsidence etc.
Concentrating of step (5) can realize by any method known in the art.This concentrating has no particular limits, and prerequisite is not destroy active component.For example, this to concentrate can be concentrating under reduced pressure.Temperature can be at about 0 ℃ to about 80 ℃, and for example approximately room temperature preferably is no more than about 60 ℃ to about 80 ℃, and for example about 0 ℃ to about 60 ℃, for example about 50-60 ℃, as about 55 ℃.Concentrating of step (5) also can be lyophilization.
A kind of common method for concentration is evaporation, for example atmospheric evaporation, reduction vaporization, thin film evaporation etc.
Can carry out drying after concentrating, for example, constant pressure and dry, drying under reduced pressure, spray drying, lyophilization etc.Dry optional step, but be preferred in certain embodiments.
In some embodiment, concentration step also can omit.For example, can directly use leachate, for example directly take, perhaps directly contain the compositions of Flos Inulae extract as feedstock production to the patient.
Water extract of the present invention (as leachate or concentrated, dry extractum afterwards) can also further carry out purification.This can adopt any or several the carrying out in many purification process known in the art.In purge process, preferably monitor the existence of active component.For example by blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve diabetics the character such as tolerance of glucose are monitored active component.
In the methods of the invention, described Flos Inulae plant can be Inula japinica or Inula britannica or their combination.
In the methods of the invention, described Flos Inulae vegetable material can be Flos Inulae plant Herb or its any part, and for example its organ, tissue or cell comprise flower, fruit, seed, stem, leaf, root, preferably the aerial parts of Flos Inulae plant, for example inflorescence.The Flos Inulae vegetable material can be a medical material, also can be crude drug.The Flos Inulae vegetable material can be tissue culture, cell culture.The Flos Inulae plant can be natural or genetically modified.The Flos Inulae vegetable material can be exsiccant or fresh.
In extracting method of the present invention, described Flos Inulae vegetable material can be through pretreated.These processing comprise physical treatment, chemical treatment, biological treatment etc.For example, the Flos Inulae vegetable material can simply wash in advance, pulverize.In one embodiment, before extracting the Flos Inulae vegetable material, can use organic solvent extraction (for example extracting simply) in advance with aqueous solvent.Organic solvent includes but not limited to: petroleum ether, chloroform, ethyl acetate, ether, methanol, ethanol, propanol, isopropyl alcohol, acetone or n-butyl alcohol or their any combination etc., as ethanol as 95% ethanol.
In the present invention, described " aqueous solvent " is any aqueous solvent, and it can be water or the aqueous solution that contains organic solvent; Wherein organic solvent can be methanol, ethanol, propanol, acetone or n-butyl alcohol or their any combination.Water can be for example distilled water, ion exchange water or tap water.Aqueous solvent can also be to add the suitably extraction water of other material.Other material can be for example organic solvent, buffer agent etc.Preferably, described aqueous solvent is water or the aqueous solution that contains organic solvent.The content of water (volume ratio) can be for example about at least 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or higher in the aqueous solvent, preferably about at least 10%, more preferably about at least 20%, 50% or more.
In a specific embodiments, Flos Inulae of the present invention (covering) flowering plant preparation method of extract be aerial parts with Flos Inulae (covering) flowering plant under room temperature, normal pressure, water or the aqueous solution lixiviate that contains organic solvent obtain Flos Inulae (covering) flower and extract extractum.Crude drug Flos Inulae (covering) flower is recommended as 1 with the w/v that soaks water or contain the aqueous solution of organic solvent: 5-15 (kg/liter).Soak time was recommended greater than 24 hours, and soaking temperature is recommended 0 ℃-60 ℃.Used organic solvent includes but not limited to methanol, ethanol, propanol, acetone and n-butyl alcohol.Concentrating can be with the extract concentrating under reduced pressure, and temperature is recommended to be equal to or less than 60 ℃ and carried out.
On the other hand, the present invention relates to the water extract (perhaps water extraction) of Flos Inulae.
" water extract (the perhaps water extraction) " of Flos Inulae of the present invention is meant the Flos Inulae extract that extracts by with aqueous solvent (as water or contain the water of organic solvent) from the Flos Inulae vegetable material, but this Flos Inulae extract contains blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improves the active component of diabetics to the tolerance of glucose.
Water extract of the present invention can obtain by the extracting method of the present invention of above definition.
On the one hand, the present invention relates to the water extract of the Flos Inulae that can obtain by any method of the present invention.
For example, can pass through decocting method, infusion process, percolation or steam distillation etc. and extract (perhaps leaching) Flos Inulae vegetable material, obtain leachate.Then, randomly concentrate, dry or purification or their any combination.
Gained extractum or purified product can further be mixed with the purpose compositions, as pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
Mainly contain alkaloid, flavonoid glycoside, peptide and carbohydrate content in the extract of the present invention.
Extract of the present invention has blood sugar lowering and hypolipemic function, and the medicine that it can be used for preparing treatment diabetes and hyperlipidemia comprises oral medicine and injection, also can be prepared as the health product of blood sugar lowering, blood fat reducing.
On the other hand, the present invention relates to contain the compositions of Flos Inulae water extract of the present invention.Preferably, said composition also contains excipient or carrier.
In specific embodiments, described compositions is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
It is known in the art that Flos Inulae water extract of the present invention is mixed with the purpose method for compositions.According to different needs, one of ordinary skill in the art of the present invention can select suitable additive, compound method, dosage form, unit dose etc.
When the present composition is pharmaceutical composition, can contain suitable pharmaceutical excipient, carrier or diluent.Can adopt the pharmaceutical field technique known to carry out preparation.
Pharmaceutical composition of the present invention can adopt multiple dosage form, and this often depends on route of administration.For example, Flos Inulae water extract of the present invention can be used for preparing the medicine of treatment diabetes and hyperlipidemia, comprises the preparation that oral medicine and parenteral are used, as injection.
Pharmaceutical composition of the present invention can pass through the number of ways administration, for example oral, parenteral etc.Dosage regimen mediating recipe amount depends on multiple factor, as route of administration, patient health situation etc., and can be determined by the doctor.Dosage range can be determined by normal experiment by those skilled in the art.
On the other hand, the compositions that the present invention relates to the Flos Inulae water extract or contain the Flos Inulae water extract is used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improves the purposes of diabetics to the medicine of the tolerance of glucose in preparation.
On the other hand, the present invention relates to Flos Inulae vegetable material or its extract (preferred water extract) is used for producing and can be used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve the purposes of diabetics to the compositions of the tolerance of glucose.Described compositions is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion preferably.
On the other hand, the present invention relates to a kind of blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve the method for diabetics, comprise to the patient giving Flos Inulae vegetable material or its extract (preferred water extract) or containing the compositions of this extract or other product after by the processing of Flos Inulae vegetable material the tolerance of glucose.
" patient " comprises any animal, preferred mammal, particularly house pet, letting animals feed.A preferred embodiment this, the patient is the people." patient " suffers from or suspects the individuality of suffering from hyperlipemia or diabetes or relevant disease, and also can be has the individuality of suffering from hyperlipemia or risk of diabetes or relevant disease, or normal individual.Therefore, Flos Inulae vegetable material of the present invention or its extract (preferred water extract) or contain the compositions of this extract or the purposes of other product after by the processing of Flos Inulae vegetable material not only comprises treatment also comprise prevention hyperlipemia or diabetes or relevant disease.
Embodiment
Embodiment 1
Flos Inulae (covering) flower (Inula japinic) inflorescence 1kg added 95% ethanol 8L soaking at room temperature 48 hours, constantly stirred.Four layers of filtered through gauze, residue continue to soak 48 hours with 95% ethanol 4L, filter.Merging filtrate, centrifugal, 45 ℃ of concentrating under reduced pressure of supernatant obtain ethanol extraction extractum 55.6 grams, and yield is 5.56%.Residue 1kg behind the ethanol extraction constantly stirs with distilled water 8L soaking at room temperature 72h.Soak filters, and residue continues to soak 24h again with distilled water 4L, filtration, merging filtrate, concentrating under reduced pressure, 55 ℃ dry the water extracted immersing paste 114.2g, yield is 11.42%.
Flos Inulae (Inula britannica) inflorescence 1kg adopts with quadrat method and extracts, and the ethanol extract yield is 5.04%, and the water extract yield is 13.01%.
Embodiment 2
Get two kinds of Flos Inulae that extract by embodiment 1 method the water extracted immersing paste a little, soluble in water, carry out thin layer chromatography relatively.
Chromatoplate: GF254 silica gel plate
Developing solvent condition 1 n-butyl alcohol: methanol: water: ammonia=3: 1: 1: 0.2
2 n-butyl alcohol: methanol: water: ammonia=2: 2: 1: 0.5
3 n-butyl alcohol: ethyl acetate: methanol: water=2: 1: 1: 2
Color condition: fluorescence developing method, λ=254nm, 365nm.
Iodine fumigation (I
2)
Tomographic results:
The Rf value of method 1 (employing condition 1):
Inula japinic water extract Inula britannica water extract
Rf 1＝0.66±0.1(365nm) Rf 1＝0.66±0.1(365nm)
Rf 2＝0.58±0.1(365，254nm，I
2) Rf 2＝0.58±0.1(365，254nm，I
2)
Rf 3＝0.52±0.1(365nm)
Rf 3＝0.49±0.1(254nm，I
2) Rf 4＝0.49±0.1(254nm，I
2)
Rf 4＝0.48±0.1(365nm) Rf 5＝0.48±0.1(365nm)
Rf 5＝0.37±0.1(I
2) Rf 6＝0.37±0.1(I
2)
Rf 6＝0.27±0.1(I
2) Rf 7＝0.27±0.1(I
2)
Rf 7＝0.20±0.1(I
2) Rf 8＝0.20±0.1(I
2)
Rf 8＝0.12±0.1(365nm) Rf 9＝0.12±0.1(365nm)
Rf 9＝0.04±0.02(254nm，I
2) Rf 10＝0.04±0.02(254nm，I
2)
Rf 10＝0.00(254nm，I
2) Rf 11＝0.00(254nm，I
2)
The Rf value of method 2 (employing condition 2):
Inula japinic water extract Inula britannica water extract
Rf 1＝0.80±0.1(365nm) Rf 1＝0.80±0.1(365nm)
Rf 2＝0.70±0.1(365nm，I
2) Rf 2＝0.70±0.1(365nm，I
2)
Rf 3＝0.65±0.1(365nm) Rf 3＝0.65±0.1(365nm)
Rf 4＝0.52±0.1(I
2) Rf 4＝0.52±0.1(I
2)
Rf 5＝0.40±0.1(I
2) Rf 5＝0.40±0.1(I
2)
Rf 6＝0.05±0.02(I
2) Rf 6＝0.05±0.02(I
2)
Rf 7＝0.00(254nm，I
2) Rf 7＝0.00(254nm，I
2)
The Rf value of method 3 (employing condition 3):
Inula japinic water extract Inula britannica water extract
Rf 1＝0.89±0.1(365nm)
Rf 1＝0.87±0.1(365nm) Rf 2＝0.87±0.1(365nm)
Rf 2＝0.76±0.1(365nm) Rf 3＝0.76±0.1(365nm)
Rf 3＝0.73±0.1(254nm，I
2) Rf 4＝0.73±0.1(254nm，I
2)
Rf 4＝0.61±0.1(365nm，I
2) Rf 5＝0.61±0.1(365nm，I
2)
Rf 5＝0.42±0.1(I
2) Rf 6＝0.42±0.1(I
2)
Rf 6＝0.28±0.1(I
2) Rf 7＝0.28±0.1(I
2)
Rf7＝0.09±0.02(I
2) Rf 8＝0.09±0.02(I
2)
Rf 8＝0.01(254nm，I
2) Rf 9＝0.00(254nm，I
2)
Tomographic results shows: the constituent of two kinds of Flos Inulae low cost vegetable plant water extracts is basic identical.Other gets standard Flos Inulae medical material 1g and makes control medicinal material solution by embodiment 1 method, puts respectively on same silica gel G 254 lamellaes and launches.Compare with control medicinal material, be same blob.
Embodiment 3
Water extract to two kinds of Flos Inulae carries out qualitative reaction, and analysis result is as follows:
Inula japinic water extract Inula britannica water extract
1 bismuth potassium iodide precipitation: (alkaloid) is positive
2 1,2,3-indantrione monohydrate chromogenic reactions: (peptide, aminoacid) is positive
The reaction of 3 alchlor fluorescence developings: (flavonoid) is positive
4 alpha-Naphthol chromogenic reactions: (saccharide) is positive
5 bromjophenol blue chromogenic reactions: (organic acid) is positive
6 ferric chloride reactions: (polyphenol and tannin) is positive
The qualitative reaction result shows in these two kinds of Flos Inulae water extracts and all contains alkaloid, peptide, flavonoid glycoside and saccharide compound, also contains polyphenol compounds such as organic acid and tannin in addition.
Embodiment 4
50 kunming mices are divided into normal control group (10) at random and treat modeling group (40).Treat that the fasting of modeling group mice (can't help water) after 24 hours, tail vein fast injection alloxan normal saline solution, dosage is 60mgkg
-1The tail point is got hematometry mouse blood sugar, blood glucose value＞20.0mmolL behind the 72h
-1Mice be chosen as diabetic mice, be divided into model group (10) (model group and normal control group feedwater), Flos Inulae (Inula japinic) water extract group (1g.kg
-1, 10) and (amount of water extract is pressed the amount of the water extract extractum of embodiment 1 and calculated) and gliclazide positive drug group (40mg/kg, 10).Gastric infusion is 18 days continuously, the 7th day mensuration blood glucose, the 18th day mensuration blood glucose.Data represent that with X ± SD two groups of mean differences are analyzed with the t check.The result shows that the Flos Inulae water extract has obvious hypoglycemic activity to the alloxan diabetes mice.
Table 1 Flos Inulae water extract is to the influence of alloxan diabetes mouse blood sugar
| Group | Blood glucose (mmol/L) | ||
| 0 day | 7 days | 18 days | |
| Normal group | 7.51±0.65 | 8.24±0.73 | 7.85±1.05 |
| Model group | 22.82±2.27 | 26.22±2.08 | 23.11±2.40 |
| The water extract group | 22.27±2.15 | 16.64±4.62*** | 12.86±6.13*** |
| The gliclazide group | 22.19±2.04 | 23.27±4.40 | 18.03±6.59* |
Numerical value=meansigma methods ± S.D. compares with model group,
*P＜0.05,
* *P＜0.001
Above diabetic mice gave the Flos Inulae water extract after 18 days, measured blood glucose, and eye socket is got blood, put to death.Measure T-CHOL, triglyceride and glycosylation albumin level in the mice serum.Data represent that with X ± SD two groups of mean differences are analyzed with the t check.Experimental result shows that this water extract has triglyceride effect in the remarkable reduction blood plasma.
Table 2 Flos Inulae water extract is to T-CHOL (TC), triglyceride (TG) in the alloxan diabetes mice plasma
Influence with glycosylation albumin (Fruc) level
| Group | TC(mmol/L) | TG(mmol/L) | Fruc(umol/L) |
| Normal group | 2.79±0.63 | 2.23±0.55 | 212.5±27.2 |
| Model group | 3.35±0.74 | 2.46±1.02 | 310.1±64.3 △△△ |
| The water extract group | 3.14±0.95 | 1.68±0.31 △* | 273.7±42.0 △△ |
| The gliclazide group | 2.93±0.69 | 2.09±0.50 | 282.4±35.7 △△ |
Numerical value=meansigma methods ± SD. is compared with normal group,
△P＜0.05,
△ △P＜0.01,
△ △ △P＜0.001; Compare with model group,
*P＜0.05.
Embodiment 5
Normal kunming mice and diabetic mice (alloxan model) (being male) fasting are after 16 hours, and normal mice is divided into three groups: matched group; Flos Inulae water extract group (1.0g/kg, po); With the metformin group (175mg/kg, po).Diabetic mice is divided into three groups equally: model group; Flos Inulae water extract (Inula japinic) group (1.0g/kg, po) (amount of water extract is pressed the amount of the water extracted immersing paste of embodiment 1 and is calculated); With the metformin group (175mg/kg, po).Every group of mice is 5.The administration group respectively in advance 1.0h give water extract and metformin, measure then and respectively organize mouse blood sugar (0min).Every group of mice oral glucose aqueous solution (2g/kg) measured and given back 30min, 60min, 90min, blood glucose when 150min and 270min.The result shows that the Flos Inulae water extract can obviously improve the tolerance of diabetic mice to glucose.
Table 3 Flos Inulae water extract is to the influence of diabetic mice glucose tolerance
| Group | Blood glucose (mmol/L) | |||||
| 0min | 30min | 60min | 90min | 150min | 270min | |
| Normal group | 4.20±0.31 | 15.32±2.96 | 13.10±2.87 | 9.04±1.58 | 5.96±0.97 | 4.10±0.53 |
| Just+revolve | 3.34±0.64 △ | 13.75±3.52 | 12.28±2.41 | 8.74±2.02 | 5.63±1.25 | 4.07±0.86 |
| Just+two | 3.11±0.58 △ | 8.84±1.63 △△ | 7.02±1.86 △△ | 5.12±0.93 △△ | 3.45±0.61 △ | 3.13±0.75 |
| Model group | 7.04±0.67 | 18.20±3.15 | 16.27±3.76 | 14.81±3.21 | 13.80±2.98 | 9.84±2.52 |
| Sugar+revolve | 7.61±1.21 | 15.92±3.23 | 11.07±2.81* | 10.44±2.67* | 7.81±2.81* | 6.52±2.30 |
| Sugar+two | 6.22±1.01 | 13.14±2.69* | 13.30±4.07 | 12.32±3.34 | 10.50±2.63 | 5.83±2.01 |
Compare with normal group,
△P＜0.05,
△ △P＜0.01; Compare with model group,
*P＜0.05.Just=and normal mice, revolve=the Flos Inulae water extract,
Two=metformin, sugar=diabetic mice.
Claims (10)
1. a method for preparing the water extract of Flos Inulae (comprising Inula japinica or Inula britannica or its combination) comprises: obtain the Flos Inulae vegetable material; Extract the Flos Inulae vegetable material with aqueous solvent, obtain the water extract of Flos Inulae.
2. the process of claim 1 wherein that extracting the Flos Inulae vegetable material with aqueous solvent comprises: (1) soaks the Flos Inulae vegetable material with aqueous solvent under proper condition; (2) separate liquid phase and solid phase; (3) randomly, repeating step (1) and (2) one or many (for example repeat 1-for several times, 1-5 time, 1-2 time, 2-5 time, 3 times, 4 times); (4) randomly, merge liquid phase; (5) randomly, will concentrate by the liquid phase that step (2) or step (4) obtain.
3. each method of claim 1-2, wherein said Flos Inulae vegetable material is the aerial parts of Flos Inulae plant, preferred inflorescence.
4. each method of claim 1-3, wherein said aqueous solvent is water or the aqueous solution that contains organic solvent; Wherein organic solvent can be methanol, ethanol, propanol, acetone or n-butyl alcohol or their any combination.
5. each method of claim 1-4, wherein before extracting the Flos Inulae vegetable material with aqueous solvent with organic solvent such as ethanol as this Flos Inulae vegetable material of ethanol extraction of 95%.
6. extract the Flos Inulae water extract that the Flos Inulae vegetable material obtains with aqueous solvent.
7. can be used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve the Flos Inulae water extract of diabetics the tolerance of glucose.
8. compositions, it contains any one Flos Inulae water extract of claim 6-7, randomly, also contains excipient or carrier; Preferably, described compositions is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
9. any one the Flos Inulae water extract or the compositions of claim 8 of claim 6-7 is used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improves the purposes of diabetics to the medicine of the tolerance of glucose in preparation.
10. Flos Inulae vegetable material or its extract (preferred water extract) are used for producing and can be used for blood sugar lowering or blood fat reducing or treatment hyperlipemia or treatment diabetes or improve the purposes of diabetics to the compositions of the tolerance of glucose, wherein preferably, described compositions is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
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| CN102240312A (en) * | 2011-07-04 | 2011-11-16 | 吉林大学 | Application of drugs by using inula britannica total flavone extract for reducing blood sugar |
| CN101209273B (en) * | 2006-12-30 | 2011-11-30 | 中国人民解放军军事医学科学院毒物药物研究所 | Preparation of inula japonica total polysaccharide and its application in pharmacy |
| CN101747153B (en) * | 2010-01-21 | 2012-07-04 | 上海交通大学 | Inula salsoloides extract and extraction method and usage thereof |
| CN102578337A (en) * | 2012-02-29 | 2012-07-18 | 张康 | Preparation method of medicinal and edible dual-purpose blood-sugar-reducing nutritious healthcare tea |
| CN109091519A (en) * | 2018-06-28 | 2018-12-28 | 吉林省中医药科学院（吉林省中医药科学院第临床医院） | The medicinal usage of inula japonica total chromocor extract |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101209273B (en) * | 2006-12-30 | 2011-11-30 | 中国人民解放军军事医学科学院毒物药物研究所 | Preparation of inula japonica total polysaccharide and its application in pharmacy |
| CN101747153B (en) * | 2010-01-21 | 2012-07-04 | 上海交通大学 | Inula salsoloides extract and extraction method and usage thereof |
| CN102240312A (en) * | 2011-07-04 | 2011-11-16 | 吉林大学 | Application of drugs by using inula britannica total flavone extract for reducing blood sugar |
| CN102578337A (en) * | 2012-02-29 | 2012-07-18 | 张康 | Preparation method of medicinal and edible dual-purpose blood-sugar-reducing nutritious healthcare tea |
| CN109091519A (en) * | 2018-06-28 | 2018-12-28 | 吉林省中医药科学院（吉林省中医药科学院第临床医院） | The medicinal usage of inula japonica total chromocor extract |
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