CN1580747A

CN1580747A - Wheat new-old rapid discriminating reagent

Info

Publication number: CN1580747A
Application number: CN 200410022560
Authority: CN
Inventors: 冯永健; 何学超
Original assignee: CHENGDU GRAIN STORAGE RESEARCH INSTITUTE STATE ADMINISTRATION OF GRAIN RESERVE; CHINESE RESERVED GRAIN MANAGEMENT GEAERAL Co
Current assignee: CHENGDU GRAIN STORAGE RESEARCH INSTITUTE STATE ADMINISTRATION OF GRAIN RESERVE; CHINESE RESERVED GRAIN MANAGEMENT GEAERAL Co
Priority date: 2004-05-19
Filing date: 2004-05-19
Publication date: 2005-02-16

Abstract

This is a distinguishing reagent used to fast distinguish the old and new wheat. The origin liquid of this reagent is compounded of methyl red, brome-phenol blue and anhydrous methyl alcohol, and is mixed with water. This reagent is not only fast distinguish between new and old wheat and also judge accurately, and its index can be quantized. It use to the wheat storage and wheat-transporting course to judge and distinguish the new wheat and old wheat.

Description

The new old quick identification reagent of wheat

Affiliated technical field

The present invention relates to be used for the new old quick identification reagent of reagent, particularly a grow wheat of the new old quick discriminating of grain.

Background technology

The new old method for quick identification of existing grain all is based on the variation of grain enzymatic activity in storage and the change mechanism of chemical constitution is set up, as guaiacol method, pH value method etc.These methods can both reasonable solution paddy and the quick problem of differentiating of rice new-old.Because the characteristic of wheat, though through many experts, technician's research effort, the quick discriminating problem of its wheat new-old degree fails to be solved preferably always.Originally change and the method set up is being used to differentiate that wheat is new when old, usually produce unusual result based on enzymatic activity, the less stable as a result of generation, what often cause that the result judges is inaccurate; Original method of setting up based on chemical composition change is used for differentiating that wheat is new can not reappear the result that document is described when old.Therefore, other problem of the new Chen Jian of wheat is undecided always, is the technical barrier that the grain industry is generally acknowledged.

Summary of the invention

The objective of the invention is to solve above-mentioned weak point of the prior art, and provide a kind of easy to use, differentiate fast, accuracy of judgement, it differentiates that color is not only clear but also stable, index can quantize, the new old quick identification reagent of the wheat that reagent corrosivity is little.

The technical measures that the present invention taked are: the new old quick identification reagent of this wheat is to be mixed by stoste and water, and wherein stoste is made up of methyl red, Bromothymol blue and absolute ethyl alcohol.In 100ml stoste, methyl red is 0.04～0.2g, and Bromothymol blue is 0.12～0.25g, and surplus is an absolute ethyl alcohol liquid.

The new old quick identification reagent of this wheat is easy to use, can identify the new Chen Chengdu of wheat quickly and accurately.So just can when wheat purchase or strange land allocation and transportation, solve the decision problem that old wheat is pretended to be Xin Mai and stores the wheat quality quality well.

The tool present embodiment

The invention will be further described below in conjunction with embodiment: get methyl red 0.04g at ambient temperature at first respectively, Bromothymol blue 0.16g together puts into graduated cylinder, and then pours anhydrous ethanol solvent into until 100ml in graduated cylinder, so just obtain stoste.Then getting 2ml stoste, to add water to 100ml again be this identification reagent finished product.This finished product reagent can be packed into by every 10ml and be sealed in the ampere bottle, bottledly goes in the carton to become kit by per 10 again.

Differentiate that with this identification reagent wheat is new when old, get earlier and differentiate that 100 of wheats put in vitro, add the quick identification reagent of 1 peace bottle (10ml) subsequently, and be interrupted and jolt, react the observation solution colour about 10 minutes:

Wheat is old by new change, and its solution colour is followed successively by:

Blueness → blue-green → green → yellow green → yellow → orange → redness

The green more wheat of solution is new more, and new wheat solution mostly is light green color; Old wheat solution is light yellow, yellow, and older person is orange or orange red; Yellow green circle in new old between, green newer of yellowish green colour cast, yellowish green colour cast Huang older.More than be to the new old qualitative analysis of wheat.

When doing the new old quantitative test of wheat, get earlier and differentiate that wheat 4.5g puts in vitro, with the quick identification reagent that adds 10ml, and be interrupted and jolt, reacted about 10 minutes, make blank with distilled water then, supernatant is measured its absorbance under 616nm:

The absorbance of new wheat is more than 0.45, and barley and wheat is new more more; The absorbance of old wheat is below 0.30, and more little wheat is old more; The wheat of absorbance between 0.30～0.45 is in new old transition period, and it is newer to be worth big person, and it is older to be worth little person.

Claims

1. new old quick identification reagent of the wheat that mixes by stoste and water, it is characterized in that stoste is made up of methyl red, Bromothymol blue and absolute ethyl alcohol, methyl red is 0.04～0.2g in 100ml stoste, and Bromothymol blue is 0.12～0.25g, and surplus is an absolute ethyl alcohol liquid.