CN1576822A - Method for measuring tree stem respiratory - Google Patents

Method for measuring tree stem respiratory Download PDF

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Publication number
CN1576822A
CN1576822A CN 03133609 CN03133609A CN1576822A CN 1576822 A CN1576822 A CN 1576822A CN 03133609 CN03133609 CN 03133609 CN 03133609 A CN03133609 A CN 03133609A CN 1576822 A CN1576822 A CN 1576822A
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China
Prior art keywords
trunk
abutment ring
air chamber
breathing
soil
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CN 03133609
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Chinese (zh)
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CN100386626C (en
Inventor
王淼
姬兰柱
郝占庆
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Institute of Applied Ecology of CAS
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Institute of Applied Ecology of CAS
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Priority to CNB031336094A priority Critical patent/CN100386626C/en
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Abstract

The present invention relates to the research of tree metabolism activity, and is especially the method of measuring tree trunk. One connecting ring is used with one end set on the surface of tree trunk and the other end connected horizontally with the soil breath air chamber. The soil breath air chamber is connected with the connecting ring via sealing sponge cushion hermetically, and the tree trunk is connected to the connecting ring with waterproof sealing silicone glue. The method is simple, precise, short in measuring period, flexible and suitable for field measurement and needs no special tree trunk breath measuring device.

Description

A kind of method of measuring the trunk breathing
Technical field
The present invention relates to research of tree metabolism activity, specifically a kind of method of measuring the trunk breathing.
Background technology
Research of tree metabolism activity is primary study trees blades all traditionally, with its index as the whole metabolism of trees.Yet respiratory activity continues to occur in all living tissues of strain trees, and wherein the trunk respiration monitoring is a most important content in the research of ecosystem carbon cycle.Carbon cycle influences ecosystem carbon and energy equilibrium, CO 2Discharging is related to following climate change, is a core content of current research on global change.It is estimated that in growing into forest trunk is breathed and to be accounted for that the carbon amount rises to 42% from 13% on total forest land.The adult trees of one strain are from the CO of xylem organization's discharging 2Amount surpasses the amount that leaf is breathed.Report is abroad arranged, poplar forest year trunk and total breathing of branch surpass 2.5 times that leaf is breathed.In photosynthetic process, total plant respiration can be lost the 30-70% of carbon assimilation.Have very significance although trunk is breathed, few people carry out the research in this field, and its main cause is exactly to determine because trunk respiration monitoring technology is difficult to resolve.Measure the trunk breathing and have many problems, many as the determining instrument annex, it is difficult to buy, and carries inconvenience, and the instrument field is installed difficult, measures limiting factors such as sample area is little.Therefore, press for the easy technical method of a kind of branch and trunk respiration monitoring.
The general open air chamber that uses a band small fan of the method for trunk breathing at present has a draft tube and an escape pipe on the air chamber, measure the CO of air inlet and escape pipe by infrared ray gas analyzer 2Variations of flux, obtain the trunk respiratory rate.Weak point is: the minute cycle is long, the device that needs the specific determination trunk to breathe, and determinator is dumb, the requirement of incompatibility field environment.
Summary of the invention
Mensuration experience accumulation by soil respiration for many years, in the research of using the soil respiration of LI6400 portable light assembly system analysis-e/or determining, answer pressing for of trunk respiration monitoring, the purpose of this invention is to provide the method that a kind of simple and easy to do mensuration trunk is breathed, its minute cycle is short, the device that does not need the specific determination trunk to breathe uses flexibly, adapts to the requirement of field environment; Also introduce the technology path that a kind of portable light assembly system assay determination instrument that can buy on the market of a widespread use carries out open-air trunk respiration monitoring.
To achieve these goals, technical scheme of the present invention is: get an abutment ring, an end is placed in trunk surface, and the other end is connected with the soil respiration air chamber horizontal seal of portable light assembly system analyser; The present invention is expanded portable light assembly system analyser technical functionality, measures trunk from the application extension of soil respiration research field to trees physiological ecological research field and breathes, and carries out forest ecology carbon cycle research.
Be tightly connected by the sponge rubber cushion between soil respiration air chamber and the abutment ring, trunk is to adopt the silicone waterproof gasket cement to be connected with abutment ring; The end that described abutment ring is connected with trunk, its cross section can be arc, adapts with the trunk surface flexure plane, the other end is smooth side grain; Described abutment ring pipe thickness is that 2~3mm is good; Described abutment ring internal diameter is 10~11.8cm; Described portable light assembly system analyser adopts the LI6400 type.
Trunk respiration monitoring technology of the present invention is compared with other prior art assay methods, has following advantage:
1. the minute cycle of the present invention is short, and the device that does not need the specific determination trunk to breathe uses flexibly, adapts to the requirement of field environment.
2. simple and easy to do.In order to obtain the spatial variations feature that trunk is breathed, the abutment ring parts that mensuration trunk of the present invention is breathed adopt the PVC material pipe, and manufacture craft is very simple, and is also very convenient with being connected of soil respiration air chamber.In addition, need the instrument accessory of usefulness few, be easy to carry, can be used for large sample and measure research, the mensuration process saves time, and is easy, credible result.
3. the present invention measures the trunk breath method and is with a wide range of applications.Minority is measured the assistive device of the technology use of trunk breathing at present, as pump, flowmeter, amplifier, an infrared ray gas analyzer, adopt assay method in the past, instrument must be placed on middle position, and selected trees must be near instrument, instrument is once the fixed position, inconvenience is moved, and annex can't supportingly be bought in addition, and the researchist must make up this system by different manufacturers.Adopting the present invention to measure trunk down with environment in any place breathes.LI6400-09 soil respiration mensuration system now can buy, and need not any adjustment both can measure the trunk breathing, does not need special attached material.
4. expand portable soil and breathed air chamber coml functions of use, the LI-6400 portable light assembly system that the present invention adopts is a kind of multi-functional analyzer, be used to measure photosynthesis and soil respiration, so the function of this instrument and performance are verified in the research work in the open air by global ecologist.Yet employing the inventive method, the user of LI6400 instrument can effectively utilize existing resource in the prior art, measuring trunk breathes, and without any need for utility appliance and special software, can carry out the mensuration of trunk breathing and trunk temperature, expand and saved spending when portable soil is breathed air chamber coml functions of use again.Show also in the expansion of its functions of use that the present invention has also realized measuring the sapwood temperature when measuring the trunk breathing.
Description of drawings
Fig. 1 is an one embodiment of the invention abutment ring scheme of installation.
Fig. 2 is an one embodiment of the invention assay method synoptic diagram.
Fig. 3 is one embodiment of the invention measurement operation figure.
Embodiment
The present invention is described in detail below in conjunction with drawings and Examples.
Embodiment 1
The experiment place is located in No. 1 study plot of Changbai Mountain forest ecosystem research station of the Chinese Academy of Sciences the 128 ° of 28 ' E in geographic position, 42 ° of 24 ' N, height above sea level 736m, 0.9~3.9 ℃ of year samming, the coldest month (January) month samming-16.7~-18.6 ℃, 17.4~20.7 ℃ of the hottest month (July) month sammings.This area belongs to the continental wettability monsoon climatic region in temperate zone, is leaved Korean Pine woods natural distributed district.Soil is dark brown forest soil, and weather belongs to the temperate continental climate that influenced by monsoon, and spring, arid was windy, and summer is burning hot and rainy, winter dryness cold, and annual rainfall 726.3mm mainly concentrates on for 6~August, about frostless season 109d.This research is chosen Changbai Mountain Korean pine forest dominant tree Korean pine, Mongolian oak as supplying the examination material.
The mensuration that trunk of the present invention is breathed: as shown in Figure 1, the abutment ring of a PVC material is placed in trunk surface, by abutment ring LI-6400-09 soil respiration air chamber (HOSC) level is placed in the trunk position again, measures trunk and breathe per unit area at soil respiration air chamber CO 2Emission flux.9.9cm be tightly connected by glue between the abutment ring of diameter soil respiration air chamber and 10.1cm internal diameter; And guarantee trunk and soil respiration air chamber be tightly connected (in soil respiration mensuration process, abutment ring is partly inserted in the soil, the soil upper surface leave 2.5cm length in case ring set on the soil respiration air chamber).
The making of abutment ring: be that one section internal diameter of perpendicular cuts pvc pipe that is 10.1cm is as abutment ring, the one end-grain cutting is cut radian and is close with the trunk surface flexure plane as far as possible, other end truncation, all otch polish smooth, and fixedly connected ring that a smooth surface then must be arranged.Therefore, remove the loose bark of trunk surface makes one section of the pvc pipe bending can be fixed on the trunk.With the silicone waterproof gasket cement abutment ring is fixed on the trunk surface, before mensuration, fix 24 hours at least, for reducing the influence of edge to measured value, will be as far as possible less with the silicone waterproof gasket cement with remove bark less, and (pipe thickness is that 2~3mm is good to use thin pvc pipe, present embodiment is 3mm), make soil respiration air chamber horizontal seal be connected the other end of abutment ring.Is connected by the sealing foam rubber cushion between this enforcement soil respiration air chamber and the abutment ring, trunk is connected with abutment ring employing silicone waterproof gasket cement.
The present invention adopts portable light assembly system analyser (LI6400-09 is produced by LI-COR company) to be used to measure the trunk breath method: open air chamber for the mobile equilibrium between assurance soil respiration air chamber and ambient atmosphere when sampling, by the gas flow of sampling pump adjusting air chamber, according to different time CO in the air chamber 2Cumulative curve calculates CO 2Respiratory rate.
LI-6400-09 soil respiration air chamber soil respiration rate calculations, need to measure the soil respiration air chamber foremost with the distance and the tested soil surface area of soil surface.The present invention calculates the effective insertion depth of soil respiration air chamber according to the soil respiration air chamber front end place of abutment ring to the volume at the fixedly connected ring of trunk place, with the surface area of trunk in ring is the soil respiration surface area, the area of trunk in abutment ring bends to stem form with cardboard, and mark comes out to cut, measure with the LI-3000 leaf area instrument that U.S. LI-COR inC company produces, the method that the present invention measures the volume of ring is: with the transverse incision of plastic sheeting sealing ring, beat a hole on the top of ring, with graduated cylinder topped up with water in ring, record the volume of PVC ring, volume experiment back sealing is being measured in this hole.The soil respiration air chamber is apart from trunk coverage (soil respiration air chamber base is to the distance of soil surface when measuring soil respiration, and unit is centimetre).
H=(V-R 2πd)/R 2π
The significant depth of input when H breathes for measuring trunk, V is the volume of abutment ring, R is the radius of abutment ring, i.e. D/2, d is that the soil respiration air chamber inserts the degree of depth in the abutment ring, as shown in Figure 2, present embodiment V=398.5cm 3R=5.05cm; D=2cm; H=2.98cm.
The result shows: the trunk temperature variation of the diurnal variation of trunk respiratory rate and seasonal variations and response has good correlativity, adopt the HOSC technology, be fit to determine the spatial variations of trunk breathing, the mensuration of breathing for trunk provides the foundation of science, can be widely used in research fields such as forest ecosystem carbon cycle.It has with oneself cheap Portability, especially is applicable to the personnel that have LI-COR6400 portable light assembly system analyser at present.
Embodiment 2
For the bigger trunk of diameter (diameter is greater than 25cm), the end that present embodiment will connecting pipe is done camber, because trunk surface is flat.
Embodiment 3
Less than 25cm, greater than the trees of 10cm, present embodiment will be made cambered surface with a section of pvc pipe for trunk diameter, makes the approaching and trunk surface of its crooked radian.
Embodiment 4
For the tree of trunk diameter less than 10cm, present embodiment is connected abutment ring with the method for axle bush expansion with the soil respiration air chamber, specifically: use the pvc pipe of 7.5 (or 5.0cm) internal diameter to be fixed on the trunk surface, the other end is connected on the abutment ring of 10.1cm internal diameter, is tightly connected with the soil respiration air chamber.
In addition, the present invention also adopts the soil respiration air chamber to measure the trunk temperature, and method is to use its temp probe, inserts in and gets final product automatic temperature measurement in the bark.Specifically be aperture of about 5cm place hand drill under abutment ring, the cambium that aperture will pass bark arrives the trunk sapwood, and it is dark to be about 1cm.The soil respiration temperature probe is inserted this hole, realize measuring and measure the sapwood temperature when trunk is breathed.With use special-purpose thermometer in the prior art, manual measurement is compared, very convenient, easy row.

Claims (7)

1. measure the method that trunk is breathed for one kind, it is characterized in that: get an abutment ring, an end is placed in trunk surface, and the other end is connected with the soil respiration air chamber horizontal seal of portable light assembly system analyser.
2. the method for breathing by the described mensuration trunk of claim 1 is characterized in that: be connected by sealing sponge rubber cushion between soil respiration air chamber and the abutment ring, trunk is connected with abutment ring employing silicone waterproof gasket cement.
3. by the method for the described mensuration trunk breathing of claim 1, it is characterized in that: the end that described abutment ring is connected with trunk, its cross section can be arc, adapts with the trunk surface flexure plane, the other end is smooth side grain.
4. the method for breathing by the described mensuration trunk of claim 1 is characterized in that: for the tree of trunk diameter less than 10cm, use the method for axle bush expansion to be connected on the abutment ring, be tightly connected with the soil respiration air chamber.
5. by the method for the described mensuration trunk breathing of claim 1, it is characterized in that: described abutment ring pipe thickness is that 2~3mm is good.
6. by the method for the described mensuration trunk breathing of claim 1, it is characterized in that: described abutment ring internal diameter is 10~11.8cm.
7. the method for breathing by the described mensuration trunk of claim 1 is characterized in that: described portable light assembly system analyser employing LI6400 type.
CNB031336094A 2003-07-29 2003-07-29 Method for measuring tree stem respiratory Expired - Fee Related CN100386626C (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100446659C (en) * 2006-12-25 2008-12-31 四川大学 Fig tree root crown moth-proofing method
CN101113943B (en) * 2006-07-28 2010-08-04 中国科学院沈阳应用生态研究所 Trees volatile organic chemistry material collecting device
CN1987421B (en) * 2006-12-27 2011-01-26 北京林业大学 Method and device for in-site detecting soil carbon dioxide flux
CN101241117B (en) * 2008-03-12 2011-05-18 福建师范大学 Air cell for determining trunk respiration
CN103033603A (en) * 2012-12-27 2013-04-10 中国热带农业科学院橡胶研究所 Packaging type connector
CN104597213A (en) * 2015-02-04 2015-05-06 国家海洋局第一海洋研究所 Trunk CO2 flux measurement device and trunk CO2 flux measurement method
CN105377020A (en) * 2014-04-16 2016-03-02 头脑木生命工学研究所 Method for surgically operating on trees
CN108776200A (en) * 2018-08-13 2018-11-09 中国林业科学研究院林业研究所 A kind of device measuring branch and root breath
CN113358826A (en) * 2021-06-10 2021-09-07 北京林业大学 Device and method for measuring tree branch and leaf respiration

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2049182B (en) * 1979-04-20 1983-03-16 Nat Res Dev Method and apparatus for sensing respiration
JP2502812B2 (en) * 1990-12-21 1996-05-29 浜松ホトニクス株式会社 Method and device for monitoring gaseous microparticles
JPH09113507A (en) * 1995-10-20 1997-05-02 Uesutoron Kk Fractional concurrent measuring method for respiration of plant and photosynthesis and its device
JPH11326314A (en) * 1998-05-08 1999-11-26 Isao Nishi Method for separating and simultaneously measuring response of respiration and photosynthesis of plant and its device
JP2927290B1 (en) * 1998-05-22 1999-07-28 新菱冷熱工業株式会社 How to diagnose plant growth from leaf respiration rate
JP2002153128A (en) * 2000-11-22 2002-05-28 Urban Front Kk Apparatus for measuring co2 consumption by tree and hermetically sealed space-forming body

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101113943B (en) * 2006-07-28 2010-08-04 中国科学院沈阳应用生态研究所 Trees volatile organic chemistry material collecting device
CN100446659C (en) * 2006-12-25 2008-12-31 四川大学 Fig tree root crown moth-proofing method
CN1987421B (en) * 2006-12-27 2011-01-26 北京林业大学 Method and device for in-site detecting soil carbon dioxide flux
CN101241117B (en) * 2008-03-12 2011-05-18 福建师范大学 Air cell for determining trunk respiration
CN103033603A (en) * 2012-12-27 2013-04-10 中国热带农业科学院橡胶研究所 Packaging type connector
CN103033603B (en) * 2012-12-27 2014-10-29 中国热带农业科学院橡胶研究所 Packaging type connector
CN105377020A (en) * 2014-04-16 2016-03-02 头脑木生命工学研究所 Method for surgically operating on trees
CN104597213A (en) * 2015-02-04 2015-05-06 国家海洋局第一海洋研究所 Trunk CO2 flux measurement device and trunk CO2 flux measurement method
CN108776200A (en) * 2018-08-13 2018-11-09 中国林业科学研究院林业研究所 A kind of device measuring branch and root breath
CN113358826A (en) * 2021-06-10 2021-09-07 北京林业大学 Device and method for measuring tree branch and leaf respiration

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