CN1576362A - Cordycepin food and cordycepin substrate and preparing process thereof - Google Patents
Cordycepin food and cordycepin substrate and preparing process thereof Download PDFInfo
- Publication number
- CN1576362A CN1576362A CN 200410059964 CN200410059964A CN1576362A CN 1576362 A CN1576362 A CN 1576362A CN 200410059964 CN200410059964 CN 200410059964 CN 200410059964 A CN200410059964 A CN 200410059964A CN 1576362 A CN1576362 A CN 1576362A
- Authority
- CN
- China
- Prior art keywords
- bottle
- cordyceps militaris
- link
- food
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The present invention is aweto food and aweto matrix and and their production process. The aweto food has simple production process and rich nutrients. The aweto food is produced with aweto matrix, cooked rice powder, milk, vegetable oil, cane sugar and additives, including xylitol, lecithin, citric acid, sodium bicarbonate, vanillin, shortening oil, salt and antioxidant. The aweto matrix is obtained through fermentation culturing in nutritive liquid inside a bottle, and the nutritive liquid is prepared with rice potato and carrot and through cooking, high pressure disinfection and fermentation.
Description
Technical field:
The present invention relates to the health food technology field, exactly it is a kind of Cordyceps militaris (L.) Link. food and Cordyceps militaris (L.) Link. matrix and preparation method thereof.
Background technology:
Cordyceps sinensis is a kind of marvellous biology, and its root is a cestode, and red Tou Hezui is arranged, and also has eight foots that align.Simultaneously grow a strain grass again,,, as plant, take root in soil just this grass ramp gets out ground whenever spring early summer not at the worm head.This is a kind of cold-resistant mixed worm that lives in the high mountain snow line area of thousands of meters of Qinghai-Tibet Plateans, is named as Chinese caterpillar fungus bat moth, and it covers with piebald on one's body, when during the warmth of spring, all the flowers bloom, just begin to seek the spouse, after the mating success, Chinese caterpillar fungus bat moth quietly dies after producing ovum in soil.Through about one month time, the moth ovum hatches not only white but also fat larva, can be just larva will grow up to do not become in, if a strain should showy with the wind fungi thecaspore be flushed to underground by cun water aloft by chance, live in seclusion the Chinese caterpillar fungus bat pretty young woman larva that passes the winter underground if come across again, it will get into and draw nutrition in the polypide.To 1 year spring early summer not.The appearance of larva remains unchanged, and in fact, its inside is nibbled totally by fungi already.Fungi slowly stretches and will come out from the head of worm, breaks through the soil, and grows the spillikin of one 4～11 cm long, and expand slightly on the top of spillikin, ovalize, surperficial red-purple, Here it is Cordyceps sinensis.As seen, a strain Cordyceps sinensis survives, and is a kind of rare good luck, and scientist is described as the excellent masterpiece of the Nature to it, and it is the combination of worm and bacterium, is again the association of animal and plant.
Chinese caterpillar fungus is one of China's tradition three big rare traditional Chinese medicines.According to the theory of traditional traditional Chinese medicine and pharmacy " integration of drinking and medicinal herbs ", successive dynasties medical science is all paid attention to utilizing diet to come preventing disease, all very emphasizes sufferer, stops weak person's dietetic regulation, and have the saying of " Diet cures more than the doctors ".
The Ancient Times in China people have had understanding very early to the pharmaceutical use of Cordyceps sinensis, and Wu Yi shows the Lip river " new compilation of materia medica " (1757) and points out that Cordyceps sinensis has the medicinal efficacy of " protecting lung kidney-nourishing, hemostasis and phlegm ".Because the nourishing and the result of treatment of Cordyceps sinensis are the most remarkable.Thereby in the supplementary Amplifications of the Compendium of Materia Medica of Zhao Xuemin, just the therapeutic action of Chinese caterpillar fungus class return for " can control weakness " in short.Zhao Xuemin say " Cordyceps sinensis is sense yin and yang 2 Qi and giving birth to, the Summer Solstice the summersolstice, so quiet be grass, Winter Solstice the winter solstice; so movingly be worm, toss about in bed to follow fortune, non-is firefly as if the corruption grass, old wheatization butterfly; feel comparable being used as medicine of damp-heat person, so can control weakness, with its gas that gets negative and positive entirely also.”
Along with the development of modern medicine, the World Health Organization predicts: enter the medical science of 21st century, and human from treatment medical science, the preventive medicine in past, develop to the sanipratics direction.Can expect that along with improving constantly of people's living standard and quality of life, people are strong to the magical and unique help of Chinese caterpillar fungus, the continuous understanding of anti-aging effects function, consumers in general will be more and more general to having the demand of Chinese caterpillar fungus functional food.Simultaneously, to preparation Chinese caterpillar fungus functional food, the demand of raw material also can be increasing.Because worm grass resources is exhausted day by day, country's explicit order taboo adopts.With the method for artificially breeding Chinese caterpillar fungus, preparation Chinese caterpillar fungus functional food has just become unique valid approach like this.
At present, the producer of domestic production Chinese caterpillar fungus functional food, obtaining on the Chinese caterpillar fungus functional component, all be with the deep fermentation method, obtain Cordyceps mycelium, then, from mycelium, extract again, and with extract or with after the mycelium pulverizing, add in proportion in the food raw material, make various Chinese caterpillar fungus functional food.Produce the factory of Chinese caterpillar fungus functional food, use Cordyceps sporophore to do almost not having of raw material extraction, simple reason is exactly that Cordyceps sporophore costs an arm and a leg, ten thousand yuan of the every kg1.7 of existing market price.So solve the raw material problem of Chinese caterpillar fungus functional food, be a restriction and an obstacle and a difficult problem that influences the development of Chinese caterpillar fungus functional food always.
At present, on the production of Chinese caterpillar fungus functional food, manufacture technology, mainly there is following shortcoming:
1, investment of production equipment is big, cost is high, uses the deep fermentation method, and the Cordyceps mycelium that obtains can not keep the distinctive awns fragrance flavor of Chinese caterpillar fungus.The functional food expert that China is famous, the Zheng Jianxian professor points out in its " functional food ": tank fermentation method, obtain Cordyceps mycelium, maximum shortcoming is that equipment has high input, the mycelium that obtains does not have the distinctive fragrance of Chinese caterpillar fungus.
2, production and processing technology complexity, effective component runs off big.Only with regard to extracting mycelial functional component,,, influence in various degree can be arranged all to the functional component of Chinese caterpillar fungus owing to the high temperature in the leaching process, pressurization, fragmentation, chemical solvent dipping.Chinese caterpillar fungus itself is worth expensive, the raw materials cost height, and extraction process complexity in addition, its functional component runs off very big, nor may extract thoroughly.The functional component complexity of Chinese caterpillar fungus own on its extraction process, should not adopt the chemical extraction method in addition, generally all uses traditional cooking process or hydrodistillation.But this method " length consuming time, energy consumption is big, cost is high, yield is low ".It should be noted that: the extraction process of modern extracting method, particularly chemical solvent, although convenient and swift, the yield height, influence is sure to the effect of extract.Decomposition reaction and degraded are the disadvantage (see Guo Jianmin " modern Chinese herbal medicine was concocted handbook Beijing Chinese medicine press 2002) of Chinese medicinal materials chemical extraction method.
3, Cordyceps polysaccharide very easily produces degraded in the extraction process process.Because polysaccharide compound, complex structure, molecular weight are big, generally all are to be formed by 10 above monose condensations of molecule, and do not have reductibility.Cordyceps polysaccharide is the topmost function composition of Chinese caterpillar fungus, currently, does not also have more effective means, can prevent the degraded of Cordyceps polysaccharide in leaching process fully.
4, present, produce preparation, popular Chinese caterpillar fungus functional food technology is still a blank.Owing to can't resolve Chinese caterpillar fungus raw material problem, complete processing complexity in addition, the Chinese caterpillar fungus functional food is produced, and only is confined to senior oral liquid, on capsule, the micropowders product, is fit to consumers in general's demand, and popular Chinese caterpillar fungus functional food does not also have manufacturer production.This problem occurs, be not that manufacturer is reluctant to produce, but also can't resolve mass-producing, the raw-material technical problem of low cost production Chinese caterpillar fungus at present.According to statistics: at present, the Chinese caterpillar fungus class functional food that the health ministry approval is produced has more than 50 kind.These product prices are mostly in hundreds of unit, and ordinary consumer be cannot afford.This promptly is an agricultural technology problem, especially a serious social benefit problem.Improving consumers in general's physical constitution, is the final purpose of producing functional health care food.Otherwise, just lost the meaning that exists of functional food.China Patent No.: 92109225 disclose a kind of name is called: the patent of invention of " novel process of artificially culturing north Chinese caterpillar fungus ", it is to be substratum with grains such as rice, add protein ingredients such as soya-bean milk, through the method for sterilizing, inoculating, sending out operations such as bacterium, kink and harvesting, produce a kind of edible mushrooms-Cordyceps militaris (L.) Link..China Patent No.: 93105056 disclose a kind of name is called: the patent of invention of " method of north worm summer in winter bacterium artificial culture sporophore ", it be with bacterial classification inoculation on solid or liquid nutrient medium, or on the silkworm chrysalis live body of inoculation, can obtain sporophore through cultivating.China Patent No.: 94115054 disclose a kind of name is called: the patent of invention of " deep layer fermenting process of Cordyceps sinensis ", it also is a kind of method of cultivating Cordyceps mycelium.
Summary of the invention:
The purpose of this invention is to provide a kind of Cordyceps militaris (L.) Link. food and Cordyceps militaris (L.) Link. matrix and its preparation method, the Cordyceps militaris (L.) Link. food that batchings such as its employing Cordyceps militaris (L.) Link. matrix and cooked rice powder, milk, vegetables oil, sucrose are made.Described Cordyceps militaris (L.) Link. matrix is lyophilize matrix or fresh Cordyceps militaris (L.) Link. fermented substrate.Described batching is ground rice crystal class food or expanded based food batching commonly used, another name for sugar, Yelkin TTS, citric acid, sodium bicarbonate, vanillin food grade,1000.000000ine mesh, shortening, salt, oxidation inhibitor.Cordyceps militaris (L.) Link. matrix prepares as follows:
A, preparation Cordyceps militaris spawn, Cordyceps militaris (L.) Link. [Cordyceps milotaris (Lex.Fr.) Link] is subordinate to Ascomycotina (Ascomycotina), gang pyrenomycetes (Pyrenomycetes), ergot Zoopagales (Clavicipitales), Clavicipitaceae (Clavicipitaceae), Cordyceps (Cordyceps) fungi.Bacterial classification is by name: pupa is drawn up mould [Paecilomyces militaris (Kob.) Brown et Smith], preservation date: on August 21st, 1988, depositary institution: Guizhou Agriculture College entomogenous fungi research department, deposit number: CGAC88-821L.Above-mentioned bacterial classification can have been bought from the market.
The Cordyceps militaris (L.) Link. liquid spawn also can prepare voluntarily, and the preparation method is as follows:
The first step, with the peeled potatoes 100g that weighs, section is gone into pot and is added water 1500ml, boils 25 minutes, remove slag get filtered juice 1000ml (as deficiency convert clear water mend add);
In second step, in the 1000ml murphy juice, be blended into: potassium primary phosphate 2.5g, sal epsom 1.5g, yeast extract paste 1.5g, peptone 5g, glucose 15g, white sugar 15g with the liquid for preparing, is sub-packed in (every bottle of 100ml) in the glass culturing bottle.
In the 3rd step,, cool off standby with the liquid culture bottle autoclaving for preparing 45 minutes.
In the 4th step,, choose the culturing bottle after about grain of rice size is put into sterilization with the Cordyceps militaris spawn that is stored in the inclined-plane pipe, lucifuge left standstill 72 hours, after generating white colony in the culturing bottle, put shaking table and cultivated 7-10 days, see and look into strain liquid and be faint yellow transperent suspension shape and can use.As plant experimentally that the back mycelia generates, annesl is normal, stroma can form about 15 days, both can confirm as excellent species.
B, the preparation culture medium, select high-quality northeast rice, potato, Radix Dauci Sativae, potato decortication section and carrot slice were boiled in 1300ml water 20～30 minutes, slightly firmly to pinch potato chips is broken being advisable, filter residue, get juice, getting the juice amount is 1000～1200ml, and insufficient section is supplied with clear water, water, potato, ratio recklessly is 1000～1200ml: 150～170g: 50～70g, the potato that previous step is rapid, Radix Dauci Sativae juice and rice mix, in 1: 0.6 ratio, in the wide-necked bottle of packing into, to install the material bottle of culture medium again, seal with polypropylene film, autoclaving cooled off standby after 35 minutes;
C, preparation fermented nutritive liquid, potato and Radix Dauci Sativae section were boiled 20～30 minutes, filter residue is got juice 1000～1200ml, the ratio of water, potato and Radix Dauci Sativae is 1000～1200ml: 200～250g: 80～100g, adds potassium primary phosphate 2～2.5g, sal epsom 1～1.5g, yeast extract paste 4～5g, fish peptone 4～5g, grape powder 15～18g, analysis for soybean powder 10～15g, B in the good filtered juice of above-mentioned filter
1And B
20.01g below, the nutritive medium for preparing is shaken up bottling, every bottle of 300～350ml, autoclaving 30 minutes cools off standby;
D, will be through planting experimentally, confirm goodly, the Cordyceps militaris (L.) Link. liquid spawn was in 1: 30 ratio, be mixed in the nutritive medium of the bacterium of going out, will be mixed with the nutritive medium of bacterial classification again, evenly be inoculated in the material bottle by every material bottle 8～10ml, postvaccinal material bottle is gone between fermentation culture the lucifuge fermentation.
E, lucifuge fermentation culture stage, incubation time 120～144 hours, 20 ℃ ± 1 ℃ of temperature control, relative humidity 60～65%, after 2 days, as seen the white colony that is scattered on the culture medium face, diameter 2mm～4mm, temperature will be guaranteed constant during this, after 5～6 days, the culture medium face is all carried out and is covered white hypha, and the bottle end is visible pale yellow white alternate, streak shape fermentating metabolism trace line;
F, astigmatic fermentation culture stage, incubation time 120～144 hours, 18 ℃ ± 1 ℃ of temperature control, relative humidity 70～75%, when seeing light, the place opens the ventilation of 1cm osculum at the material bottle closure; Observe the culture medium face: see light after 2 days, visible mycelia is ivory white or light yellow by white commentaries on classics, and after 5～6 days, big portion changes pale yellow, and bottle bottom fermentation metabolism trace spot is consistent with material bottle face;
G, light filling fermentation increase the stage of supporting: 192～216 hours time, every day post sunset, mend according to fluorescent lamp, the daybreak to next day, intensity of illumination is no less than 1500LX, 20 ℃ ± 1 ℃ of temperature control, relative humidity 75%, observe the culture medium face: after 2 days, visible mycelia is orange, the material bottle bottom of commentaries on classics obviously, fermentating metabolism trace spot is consistent with material bottle face, after 8 days, mycelia is the volt shape of crawling, orange, there are indivedual buttons to occur, even the sporophore stroma appears, and material bottle bottom metabolism trace spot, striped broadens, be block, showing this moment ferments finishes;
H, gather: with the substratum plastid that ferments, take out, will have the material piece of mildew phenomena to abandon out from the material bottle, behind hygiene inspection, by kind requirements of making functional food go into respectively freezer or directly batching process.To shifting to an earlier date 2 days between fermentation culture with 5～8g/m2 sulphur fumigation 24 hours, behind the emptying gas, put the material bottle again, the material bottle will keep more than the 40cm apart from ground, and every buttress is stacked 3～4 layers will make the room air circulation.
Advantage of the present invention is:
1, technological process is simple, and is economical and practical, on the raw material that obtains preparation Chinese caterpillar fungus functional food, all takes from a kind of raw material-rice.Making rice is the raw material of preparation Chinese caterpillar fungus fermentation matrix body, simultaneously, when it through cultivation and fermentation, generate the certain content of metabolism, behind the Chinese caterpillar fungus functional component, again by directly as, the major ingredient of preparation Chinese caterpillar fungus functional food uses.So just simplified production process, saved big content of starting materials again, reduced cost significantly than past.Owing to use present technique, without extraction process, manufacturing enterprise also need not invest expensive extraction processing units, has saved substantial contribution input (the extraction equipment of general scale is all in millions of units).
2, the solid fermentating mode of present technique employing, Cordyceps militaris (L.) Link. fungus is on the rice matrix, carry out the biochemical metabolism reaction, the Chinese caterpillar fungus functional food of producing, its functional component, be to exist with state of nature, it is complete, kept the aromatic odour and the nutritive element of Chinese caterpillar fungus self uniqueness naturally, and the functional component nature of Chinese caterpillar fungus day is become.Because of there not being the machining process of extraction, just there is not the artificial destruction of effective component loss and effective ingredient yet, we can say, be a kind of green food worthy of the name.
3, operative technique is simple, and the preparation material is easy to get.Use present technique and produce the Chinese caterpillar fungus functional food, can implement in conventional middle and small scale edible mushrooms factory or food factory.Except that formulation and administrative skill standard, big portion is skilled work.Preparation Chinese caterpillar fungus functional food raw material mainly is rice, potato and a spot of nutrient substance.As long as note the places of origin of raw materials and producer's quality product.Utilization present technique exploitation Chinese caterpillar fungus functional food also is a deep processing of farm products, improves agricultural product added value, has opened up a new road.
4, the fermentation administrative skill of " in real time monitoring ", overcome traditional, the main increase and decrease organic or inorganic chemical dose that relies on, regulate and control the variation of Chinese caterpillar fungus fermentation culture medium, cause fermenting process to be difficult for grasping, fermentation culture matrix is perishable, easily scleroma has technical problems such as peculiar smell.The Nutrious fermented prescription of utilization present technique preparation is implemented the fermentation management process of " monitoring in real time ", and it is perishable to have solved Chinese caterpillar fungus fermentation matrix, and easily scleroma has technical problems such as peculiar smell.Make the Chinese caterpillar fungus culture medium of fermentation, after obtaining ideal Chinese caterpillar fungus functional component, both kept the distinctive aromatic odour of Chinese caterpillar fungus, again can be by direct processing and utilization.
5, use present technique, but process for processing, and adaptation consumers in general levels of actual consumption, popular, popular Chinese caterpillar fungus functional food-Chinese caterpillar fungus reconstitutes food such as ground rice crystalline substance and Chinese caterpillar fungus leisure rice cracker.Opened up functional health care food to popular, the new road that the universalness direction develops.Make the Chinese caterpillar fungus functional food that is rich in nutritive value, be dissolved among the daily bread, become popular, universalness functional food.
6, the Cordyceps militaris (L.) Link. food with the present invention's preparation possesses very high nutritive value, through protein, sugar and the lipid content of testing identity Cordyceps militaris (L.) Link. a little less than Cordyceps sinensis, and outside the amino acid whose content Gelucystine, the content of the content that all is higher than Cordyceps sinensis, particularly bacterium propylhomoserin, knot propylhomoserin, Isoleucine, leucine, phenylalanine, Methionin, tryptophane shows 6.2,5.3,5.1,4.9,3.8,5.2,11.6 times of summer morning in winter grass content respectively.Various in addition VITAMIN and mineral substance and cordycepic acid, content of cordycepin all are higher than the content of Cordyceps sinensis.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
Embodiment:
Embodiment 1: the preparation of Cordyceps militaris (L.) Link. matrix
A, choose and plant experimentally Cordyceps militaris spawn, choose pupa and draw up mould [Paecilomycesmilitaris (Kob.) Brown et Smith] bacterial classification, as plant experimentally the back mycelia and generate, annesl is normal, and stroma can form about 15 days, both can confirm as excellent species.
B, the preparation culture medium, select high-quality northeast rice, potato, Radix Dauci Sativae, potato decortication section and carrot slice were boiled in 1300ml water 20～30 minutes, slightly firmly to pinch potato chips is broken being advisable, filter residue, get juice, getting the juice amount is 1000～1200ml, and insufficient section is supplied with clear water, water, potato, ratio recklessly is 1000～1200ml: 150～170g: 50～70g, the potato that previous step is rapid, Radix Dauci Sativae juice and rice mix, in 1: 0.6 ratio, in the wide-necked bottle of packing into, to install the material bottle of culture medium again, seal with polypropylene film, autoclaving cooled off standby after 35 minutes;
C, preparation fermented nutritive liquid, potato and Radix Dauci Sativae section were boiled 20～30 minutes, filter residue is got juice 1000～1200ml, the ratio of water, potato and Radix Dauci Sativae is 1000～1200ml: 200～250g: 80～100g, adds potassium primary phosphate 2～2.5g, sal epsom 1～1.5g, yeast extract paste 4～5g, fish peptone 4～5g, grape powder 15～18g, analysis for soybean powder 10～15g, B in the good filtered juice of above-mentioned filter
1And B
20.01g below, the nutritive medium for preparing is shaken up bottling, every bottle of 300～350ml, autoclaving 30 minutes cools off standby;
D, will be through planting experimentally, confirm goodly, the Cordyceps militaris (L.) Link. liquid spawn was in 1: 30 ratio, be mixed in the nutritive medium of the bacterium of going out, will be mixed with the nutritive medium of bacterial classification again, evenly be inoculated in the material bottle by every material bottle 8～10ml, postvaccinal material bottle is gone between fermentation culture the lucifuge fermentation.
E, lucifuge fermentation culture stage, incubation time 120～144 hours, 20 ℃ ± 1 ℃ of temperature control, relative humidity 60～65%, after 2 days, as seen the white colony that is scattered on the culture medium face, diameter 2mm～4mm, temperature will be guaranteed constant during this, after 5～6 days, the culture medium face is all carried out and is covered white hypha, and the bottle end is visible pale yellow white alternate, streak shape fermentating metabolism trace line;
F, astigmatic fermentation culture stage, incubation time 120～144 hours, 18 ℃ ± 1 ℃ of temperature control, relative humidity 70～75%, when seeing light, the place opens the ventilation of 1cm osculum at the material bottle closure; Observe the culture medium face: see light after 2 days, visible mycelia is ivory white or light yellow by white commentaries on classics, and after 5～6 days, big portion changes pale yellow, and bottle bottom fermentation metabolism trace spot is consistent with material bottle face;
G, light filling fermentation increase the stage of supporting: 192～216 hours time, every day post sunset, mend according to fluorescent lamp, the daybreak to next day, intensity of illumination is no less than 1500LX, 20 ℃ ± 1 ℃ of temperature control, relative humidity 75%, observe the culture medium face: after 2 days, visible mycelia is orange, the material bottle bottom of commentaries on classics obviously, fermentating metabolism trace spot is consistent with material bottle face, after 8 days, mycelia is the volt shape of crawling, orange, there are indivedual buttons to occur, even the sporophore stroma appears, and material bottle bottom metabolism trace spot, striped broadens, be block, showing this moment ferments finishes;
H, gather: with the substratum plastid that ferments, take out, will have the material piece of mildew phenomena to abandon out from the material bottle, behind hygiene inspection, by kind requirements of making functional food go into respectively freezer or directly batching process.
Embodiment 2:
A, preparation Cordyceps militaris (L.) Link. liquid spawn,
The first step is weighed peeled potatoes, and section is gone into pot and added water, boils 25 minutes, removes slag to get filtered juice (converting the clear water benefit as deficiency adds); Potato and water add the 1500ml ratio in every 100g and go into pot, and filtered juice is 1000ml.
In second step, in every 1000ml murphy juice, be blended into: potassium primary phosphate 2.5g, sal epsom 1.5g, yeast extract paste 1.5g, peptone 5g, glucose 15g, white sugar 15g with the liquid for preparing, is sub-packed in (every bottle of 100ml) in the glass culturing bottle.
In the 3rd step,, cool off standby with the liquid culture bottle autoclaving for preparing 45 minutes.
In the 4th step,, choose the culturing bottle after about grain of rice size is put into sterilization with the Cordyceps militaris spawn that is stored in the inclined-plane pipe, lucifuge left standstill 72 hours, after generating white colony in the culturing bottle, put shaking table and cultivated 7-10 days, see and look into strain liquid and be faint yellow transperent suspension shape and can use.As plant experimentally that the back mycelia generates, annesl is normal, stroma can form about 15 days, both can confirm as excellent species.Other is with embodiment 1.
Embodiment 3: Chinese caterpillar fungus reconstitutes the preparation of ground rice crystalline substance
Major ingredient: use culture medium powder through lyophilize, superfine grinding.More than granularity 200 orders.
Dosage: lyophilize matrix powder 100g, cooked rice powder 100kg (granularity 200 orders), another name for sugar 20kg, Yelkin TTS 200g, vegetables oil 4kg, citric acid 200g, sodium bicarbonate 200g, vanillin food grade,1000.000000ine mesh 200g.
Working method:, press the spray-drying process processes with the raw material for preparing.Packaging means and wrapping material: 40g pouch, vacuum pack with nitrogen, every sack 18 pouches, net weight 720g.Wrapping material aluminium epistasis plastics.
Embodiment 4: the preparation of Chinese caterpillar fungus rice cracker
Major ingredient: use fresh, the Chinese caterpillar fungus fermentation culture medium of gathering the same day.
Dosage: fresh Chinese caterpillar fungus fermentation matrix 100kg, ripe rice 200kg (weight in wet base, moisture 60%), multitudinous sugared 10kg, shortening 20kg, salt 4kg, pure fresh milk 5kg, lemon 20g, sodium bicarbonate 400g, the oxidation inhibitor 20g of boiling.
The sauce dosage: pure water 1000ml, Zulkovsky starch 400g, gelatinum 1.5g, salt 500g, vanillin food grade,1000.000000ine mesh 250g (as make spiced flavor, can add spice 250g, remove the multitudinous sugar in the batching).
Working method: a, will prepare load weighted material product earlier, in the ramming machine of packing into, ramming becomes the mud shape; B, material product that ramming is good, the shaper of packing into is squeezed into the strip and block of being convenient to next step section usefulness; C, with the material product of moulding, placed between cold the putting of 2 ℃～4 ℃ of temperature 12 hours; D, with the material product of cold postpone, last slicing machine or molding machine be processed into thick 1.5mm, each 15mm of length and width to the square of 20mm or diameter 15mm to 20mm, diamond pattern, roundly all can; E, go into dryer, under the condition of 60 ℃～70 ℃ of temperature, between oven dry moisture content to 25%～30%; F will become the material product of leather hard, go into the high temperature baking oven, and will be under 200 ℃～260 ℃ conditions of temperature, roasting to expecting that product swell, shortcake is ripe.G, treat material product come out of the stove after, when surface temperature is reduced to 30 ℃, adopt spraying method or coating method, the even spray material product of sauce are surperficial, go into the drying machine drying then; H, under 70 ℃～80 ℃ conditions of drying temperature, make that the material product are moisture comes out of the stove when following 8%.After treating the finished product cooling, immediately pack.Adopt vacuum pack with nitrogen.
Claims (5)
1, Cordyceps militaris (L.) Link. food and Cordyceps militaris (L.) Link. matrix and preparation method thereof is characterized in that: the Cordyceps militaris (L.) Link. food that batchings such as its employing Cordyceps militaris (L.) Link. matrix and cooked rice powder, milk, vegetables oil, sucrose are made.
2, Cordyceps militaris (L.) Link. food according to claim 1 is characterized in that: described Cordyceps militaris (L.) Link. matrix is lyophilize matrix or fresh Cordyceps militaris (L.) Link. fermented substrate.
3, Cordyceps militaris (L.) Link. food according to claim 1 is characterized in that: described batching is ground rice crystal class food or expanded based food batching commonly used, another name for sugar, Yelkin TTS, citric acid, sodium bicarbonate, vanillin food grade,1000.000000ine mesh, shortening, salt, oxidation inhibitor.
4, a kind of preparation method of Cordyceps militaris (L.) Link. matrix as claimed in claim 1 is characterized in that: Cordyceps militaris (L.) Link. matrix prepares as follows:
The selection of a, liquid Cordyceps militaris spawn, Cordyceps militaris spawn can be chosen or preparation voluntarily, and the preparation method is as follows voluntarily: the first step, and the peeled potatoes section of weighing is gone into pot and added poach 25 minutes, remove slag and get filtered juice, convert clear water as deficiency and mend and add; Potato and water add the 1500ml ratio in every 100g and go into pot, and filtered juice is 1000ml;
In second step, in every 1000ml murphy juice, be blended into: potassium primary phosphate 2.5g, sal epsom 1.5g, yeast extract paste 1.5g, peptone 5g, glucose 15g, white sugar 15g with the liquid for preparing, is sub-packed in the glass culturing bottle every bottle of 100ml;
In the 3rd step,, cool off standby with the liquid culture bottle autoclaving for preparing 45 minutes;
In the 4th step,, choose the culturing bottle after about grain of rice size is put into sterilization with the Cordyceps militaris spawn that is stored in the inclined-plane pipe, lucifuge left standstill 72 hours, after generating white colony in the culturing bottle, put shaking table and cultivated 7-10 days, see and look into strain liquid and be faint yellow transperent suspension shape and can use.As plant experimentally that the back mycelia generates, annesl is normal, stroma can form about 15 days, both can confirm as excellent species;
B, the preparation culture medium, select high-quality northeast rice, potato, Radix Dauci Sativae, potato decortication section and carrot slice were boiled in 1300ml water 20～30 minutes, slightly firmly to pinch potato chips is broken being advisable, filter residue, get juice, getting the juice amount is 1000～1200ml, and insufficient section is supplied with clear water, water, potato, ratio recklessly is 1000～1200ml: 150～170g: 50～70g, the potato that previous step is rapid, Radix Dauci Sativae juice and rice mix, in 1: 0.6 ratio, in the wide-necked bottle of packing into, to install the material bottle of culture medium again, seal with polypropylene film, autoclaving cooled off standby after 35 minutes;
C, preparation fermented nutritive liquid, potato and Radix Dauci Sativae section were boiled 20～30 minutes, filter residue is got juice 1000～1200ml, the ratio of water, potato and Radix Dauci Sativae is 1000～1200ml: 200～250g: 80～100g, adds potassium primary phosphate 2～2.5g, sal epsom 1～1.5g, yeast extract paste 4～5g, fish peptone 4～5g, grape powder 15～18g, analysis for soybean powder 10～15g, B in the good filtered juice of above-mentioned filter
1And B
20.01g below, the nutritive medium for preparing is shaken up bottling, every bottle of 300～350ml, autoclaving 30 minutes cools off standby;
D, will be through planting experimentally, confirm goodly, the Cordyceps militaris (L.) Link. liquid spawn was in 1: 30 ratio, be mixed in the nutritive medium of the bacterium of going out, will be mixed with the nutritive medium of bacterial classification again, evenly be inoculated in the material bottle by every material bottle 8～10ml, postvaccinal material bottle is gone between fermentation culture the lucifuge fermentation.
E, lucifuge fermentation culture stage, incubation time 120～144 hours, 20 ℃ ± 1 ℃ of temperature control, relative humidity 60～65%, after 2 days, as seen the white colony that is scattered on the culture medium face, diameter 2mm～4mm, temperature will be guaranteed constant during this, after 5～6 days, the culture medium face is all carried out and is covered white hypha, and the bottle end is visible pale yellow white alternate, streak shape fermentating metabolism trace line;
F, astigmatic fermentation culture stage, incubation time 120～144 hours, 18 ℃ ± 1 ℃ of temperature control, relative humidity 70～75%, when seeing light, the place opens the ventilation of 1cm osculum at the material bottle closure; Observe the culture medium face: see light after 2 days, visible mycelia is ivory white or light yellow by white commentaries on classics, and after 5～6 days, big portion changes pale yellow, and bottle bottom fermentation metabolism trace spot is consistent with material bottle face;
G, light filling fermentation increase the stage of supporting: 192～216 hours time, every day post sunset, mend according to fluorescent lamp, the daybreak to next day, intensity of illumination is no less than 1500LX, 20 ℃ ± 1 ℃ of temperature control, relative humidity 75%, observe the culture medium face: after 2 days, visible mycelia is orange, the material bottle bottom of commentaries on classics obviously, fermentating metabolism trace spot is consistent with material bottle face, after 8 days, mycelia is the volt shape of crawling, orange, there are indivedual buttons to occur, even the sporophore stroma appears, and material bottle bottom metabolism trace spot, striped broadens, be block, showing this moment ferments finishes;
H, gather: with the substratum plastid that ferments, take out, will have the material piece of mildew phenomena to abandon out from the material bottle, behind hygiene inspection, by kind requirements of making functional food go into respectively freezer or directly batching process.
5, the preparation method of Cordyceps militaris (L.) Link. matrix according to claim 4, it is characterized in that: to shifting to an earlier date 2 days between fermentation culture with 5～8g/m2 sulphur fumigation 24 hours, behind the emptying gas, put the material bottle again, the material bottle will keep more than the 40cm apart from ground, and every buttress is stacked 3～4 layers will make the room air circulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410059964 CN1576362A (en) | 2003-06-26 | 2004-06-25 | Cordycepin food and cordycepin substrate and preparing process thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03133807A CN1462591A (en) | 2003-06-26 | 2003-06-26 | Food and substrate of cordyceps militatris, and preparing method |
| CN03133807.0 | 2003-06-26 | ||
| CN 200410059964 CN1576362A (en) | 2003-06-26 | 2004-06-25 | Cordycepin food and cordycepin substrate and preparing process thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1576362A true CN1576362A (en) | 2005-02-09 |
Family
ID=34593034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410059964 Pending CN1576362A (en) | 2003-06-26 | 2004-06-25 | Cordycepin food and cordycepin substrate and preparing process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1576362A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165163B (en) * | 2007-10-17 | 2010-06-16 | 杨国栋 | Method for preparing fowl ovum like culture medium and application thereof |
| CN102172273A (en) * | 2011-03-09 | 2011-09-07 | 王永显 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
| CN103172424A (en) * | 2011-12-26 | 2013-06-26 | 江苏康迪生物科技有限公司 | Preparation method of culture solution for breeding organic Cordyceps militaris strain |
| CN103416223A (en) * | 2013-08-07 | 2013-12-04 | 中南林业科技大学 | Method for improving cordycepin output in cordyceps militaris fermentation broth |
| CN104387141A (en) * | 2014-09-30 | 2015-03-04 | 衡水为尔虫草生物科技开发有限公司 | Cordyceps militaris cultivation liquid |
| CN105286007A (en) * | 2015-09-11 | 2016-02-03 | 雷本龙 | Health-care food |
-
2004
- 2004-06-25 CN CN 200410059964 patent/CN1576362A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165163B (en) * | 2007-10-17 | 2010-06-16 | 杨国栋 | Method for preparing fowl ovum like culture medium and application thereof |
| CN102172273A (en) * | 2011-03-09 | 2011-09-07 | 王永显 | Method for producing cordyceps militaris fermentation rice by utilizing solid state fermentation of rice |
| CN103172424A (en) * | 2011-12-26 | 2013-06-26 | 江苏康迪生物科技有限公司 | Preparation method of culture solution for breeding organic Cordyceps militaris strain |
| CN103416223A (en) * | 2013-08-07 | 2013-12-04 | 中南林业科技大学 | Method for improving cordycepin output in cordyceps militaris fermentation broth |
| CN103416223B (en) * | 2013-08-07 | 2015-05-13 | 中南林业科技大学 | Method for improving cordycepin output in cordyceps militaris fermentation broth |
| CN104387141A (en) * | 2014-09-30 | 2015-03-04 | 衡水为尔虫草生物科技开发有限公司 | Cordyceps militaris cultivation liquid |
| CN105286007A (en) * | 2015-09-11 | 2016-02-03 | 雷本龙 | Health-care food |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101279959B1 (en) | Manufacturing Method of Fermented Materials using Wax Gourd and Functional food of Thereof Manufacturing | |
| CN101215527A (en) | Method for cultivating silkworm chrysalis Cordyceps sinensis | |
| CN102405763A (en) | Cultivating method of aweto | |
| JP4616413B1 (en) | Fermented food manufacturing method and fermented food | |
| CN1098244A (en) | Cultural method of edible mushroom and processing and utilization thereof | |
| CN103636396B (en) | The implantation methods of a kind of water east leaf mustard | |
| CN107593955A (en) | Ferment edible mushroom tea preparation process and fermentation edible mushroom tea | |
| KR20190059063A (en) | Nosh for puppies and method for manufacturing the same | |
| CN103283608A (en) | Factory cultivation strains of needle mushrooms, and cultivation method thereof | |
| CN1091149C (en) | Method for cultivating Chinese caterpillar fungus | |
| CN1462591A (en) | Food and substrate of cordyceps militatris, and preparing method | |
| KR101027964B1 (en) | Process for preparing functional food comprising Goami rice cultivated with mixed pharmaceutical mushrooms | |
| CN102177812A (en) | Method for increasing yield of volvariella volvacea | |
| KR20020084970A (en) | Healthy fermentation food using natural medicinal plants and process for preparation thereof | |
| CN1576362A (en) | Cordycepin food and cordycepin substrate and preparing process thereof | |
| CN106336992A (en) | Purple sweet potato wine with beauty maintaining and body slimming effects | |
| CN1454987A (en) | Medicinal-cultured cordyceps sinensis, and cultivating method and use thereof | |
| CN108373347A (en) | A kind of manufacturing technology of section of wooden culture medium of edible fungus | |
| CN101240248A (en) | Edible fungi sclerotium oyster mushroom strain and application thereof | |
| CN112314918A (en) | Cordyceps sinensis and chestnut paste | |
| CN106071561A (en) | A kind of Fructus Lycopersici esculenti Cordyceps liquid spawn converts beverage | |
| CN100496269C (en) | Harmonious Pu'er tea | |
| CN1561684A (en) | Planting technology for zibaoxiangnuo No.1 rice and its developing application | |
| CN104521549B (en) | A kind of method of artificial cultivation platform mushroom disk mushroom | |
| CN103932032B (en) | Biological flour preparation method containing Cordyceps sinensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |