CN1548141A

CN1548141A - Chinese medicine composition for treating endometriosis and its prepn process

Info

Publication number: CN1548141A
Application number: CNA031365930A
Authority: CN
Inventors: 李光荣; 肖伟; 戴翔翎; 凌娅; 沈静; 徐玉玲; 刘晓东
Original assignee: Jiangsu Kanion Pharmaceutical Co Ltd
Current assignee: Jiangsu Kanion Pharmaceutical Co Ltd
Priority date: 2003-05-22
Filing date: 2003-05-22
Publication date: 2004-11-24
Anticipated expiration: 2023-05-22
Also published as: CN1244341C

Abstract

The Chinese medicine composition for treating endometriosis is prepared with eight kinds of Chinese medicinal materials, including trogopterus dung, lindera root, angelica, red peony root, motherwort, etc. During its preparation, different extraction methods, including decoction, distillation and ethanol extraction, are adopted to different materials to extract the effective components fully. The Chinese medicine composition has high curative effect in treating endometriosis.

Description

A kind of Chinese medicine composition for the treatment of endometriosis and preparation method thereof

Invention field

The present invention relates to a kind of Chinese medicine composition, particularly be used for the treatment of the Chinese medicine composition of endometriosis, relate to the preparation method of said composition simultaneously.

Background technology

Endometriosis is by the caused a kind of pathological changes of endometrium that is grown in cavity of uterus position in addition.Because ectopic endometrium still is subjected to the influence of ovarian hormone, through preceding hyperemia, menstrual period are hemorrhage, and accumulation of blood can cause that focus occurs inflammatory reaction on every side and stimulates local organization dysmenorrhea to occur in tissue.

According to statistics, the endometriosis sickness rate accounts for childbearing age women's 15%-20%.It mainly shows as secondary dysmenorrhea, sexual intercourse tumor, pelvic pain, irregular menstruation, infertile etc.The generation rate of malignant tumour that endometriosis causes is about 0.7%-1%, and the main malignant tumor that forms grows on the ovary.

At present, also do not treat the active drug of endometriosis on the market, doctor trained in Western medicine generally adopts hormonotherapy or operative treatment, patient recovery is longer, and in therapeutic process, also bring huge misery, so extensive patients is called taking convenience, onset is rapid, toxicity is low medicine to patient.

Summary of the invention

One object of the present invention is to disclose a kind of Chinese medicine composition of new endometriosis; Another object of the present invention is the method for a kind of new treatment endometriosis Chinese medicine composition of open preparation.

The crude drug of pharmaceutical composition of the present invention is formed and proportioning following (by weight):

The A compositions:

Oletum Trogopterori 170-230 weight portion Radix Linderae 135-190 weight portion Radix Angelicae Sinensis 135-190 weight portion

Radix Paeoniae Rubra 135-190 weight portion Herba Leonuri 135-190 weight portion Myrrha 135-190 weight portion

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion

The present invention forms in the A compositions on the basis of general idea under the guidance of Chinese medical theory, and plus-minus forms following B, C, D, E compositions.

The B compositions:

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion Ramulus Cinnamomi 135-190 weight portion

Radix Aconiti Lateralis Preparata 135-190 weight portion Radix Salviae Miltiorrhizae 390-440 weight portion Rhizoma Corydalis 135-190 weight portion

Flos Carthami 180-220 weight portion Radix Paeoniae Alba 80-120 weight portion.

The preferred Radix Aconiti Lateralis Preparata of Radix Aconiti Lateralis Preparata.

The C compositions:

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion Cortex Cinnamomi 135-190 weight portion

Radix Aconiti 135-190 weight portion Cortex Moutan 416.67g Lignum Sappan 135-190 weight portion

Flos Carthami 180-220 weight portion rhizoma sparganic 80-120 weight portion.

The preferred Radix Aconiti Preparata of Radix Aconiti.

The D compositions:

Radix Aconiti Lateralis Preparata 135-190 weight portion Radix Salviae Miltiorrhizae 390-440 weight portion Lignum Sappan 135-190 weight portion

Rhizoma Curcumae 180-220 weight portion rhizoma sparganic 80-120 weight portion.

The E compositions:

Rhizoma Curcumae 180-220 weight portion rhizoma sparganic 80-120 weight portion

The above-mentioned prescription medicine of the present invention can add excipient, is prepared into pharmaceutical dosage form commonly used according to the preparation process of routine, for example, and pill, tablet, granule, oral liquid, capsule, masticatory etc.

The capsule preparation method thereof of A, pharmaceutical composition is: above eight flavors, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 8-12 doubly measures 60-80% and extract 1-3 time, each 1-3 hour, filter, merge filtrate twice, get filtrate 1. ,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Herba Leonuri adds 5-7 and doubly measures 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, merge filtrate twice, get filtrate 2.,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Herba Leonuri medicinal residues and Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merge three times filtrate, filtrate 3., the clear paste that to be concentrated into 80-90 ℃ of density be 1.08-1.10 adds ethanol and makes and contain the alcohol amount and reach 60-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, continue to concentrate the 80-90 ℃ of clear paste that density is 1.08-1.10; Radix Angelicae Sinensis adds 5-7 times of water gaging, soaked 60 minutes, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, filtrate 4., 4000-6000r/min is centrifugal, gets supernatant, the clear paste that to be concentrated into 80-90 ℃ of density be 1.08-1.10; The medicinal residues that Radix Angelicae Sinensis, Rhizoma Curcumae extract behind the volatile oil add 8 times of ethanol of measuring 85-95%, reflux, extract, 2-3 time, each 1-2 hour, filter, merge filtrate twice, get filtrate 5., filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 4-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and gets the ZHG1 granule, or encapsulated, gets the ZHG1 capsule.The capsule preparation method thereof of B, pharmaceutical composition is: Rhizoma Corydalis, the Radix Paeoniae Alba, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 8-12 doubly measures 60-80% and extract 1-3 time, each 1-3.0 hour, filter, merging filtrate, filtrate 1.-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 60-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time, each 1-2 hour, filter, 5. merging filtrate gets filtrate, filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 4-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 40-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets the ZH62 capsule.The capsule preparation method thereof of C, pharmaceutical composition is: Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 8-12 doubly measures 65-80% and extract 1-3 time, each 1-3 hour, filter, merging filtrate, get filtrate 1.,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri add 6-7 and doubly measure 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri medicinal residues and Radix Aconiti Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 65-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Cortex Cinnamomi, Radix Angelicae Sinensis, Flos Carthami extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time, each 1-2 hour, filter, 5. merging filtrate gets filtrate, filtrate-0.09～-0.06MPa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 4-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets the ZH63 capsule.The capsule preparation method thereof of D, pharmaceutical composition is: Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 9-11 doubly measures 60-75% and extract 1-3 time, each 1-3 hour, filter, merging filtrate, get filtrate 1.,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 5-7 and doubly measure 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 65-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time, each 1-2 hour, filter, 5. merging filtrate gets filtrate, filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 4-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets the ZHG4 capsule.The capsule preparation method thereof of E, pharmaceutical composition is: Rhizoma Corydalis, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 9-11 doubly measures 65-80% and extract 1-3 time, each 1-3 hour, filter, merging filtrate, get filtrate 1.,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 5-7 and doubly measure 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 65-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Cortex Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time each 1-2 hour, filters, merging filtrate, get filtrate 5., filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 5-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets ZHG5.

Present composition preparation has tangible blood circulation promoting and blood stasis dispelling, analgesia, antiinflammatory and to endometriotic inhibitory action, taking convenience, and onset is rapid, and toxicity is low.

Following experimental example is used to further specify the present invention.ZHG1 granule, ZHG2 granule, ZHG3 granule, ZHG4 granule, ZHG5 granule are respectively the granular preparation of above-mentioned A-E compositions.

Experimental example 1 present composition preparation pharmacodynamics test

1, experiment material: 1.1 animals: SD kind rat, female, body weight 250-280 gram, SPF level, the animal quality certification number: SCXK (capital) 2002-0003; Rabbit: female, New Zealand's kind, 2-2.5Kg, the I level, the animal credit number: No. 024 total No. 069, all available from Beijing Vital River Experimental Animals Technology Co., Ltd..Kunming mouse, female, body weight 18-20 gram, available from animal housing of Beijing Biological Product Inst., animal credit number: (1999) No. 016.1.2 instrument LG-R-80 series blood viscosity meter, Beijing generation Supreme Being instrument company produces.Hot plate dolorimeter GJ-8420 type, Chinese Zhejiang.1.3 medicine: ZHG1 granule, ZHG2 granule, ZHG3 granule, ZHG4 granule, ZHG5 granule, Kangyuan Pharmaceutical Co., Ltd., Jiangsu Prov provides; The danazol capsule: the 200mg/ grain, Jiangsu Lianhuan Pharmaceutical Co., Ltd. produces, lot number: 20020401.Diethylstilbestrol: 1mg/ml, lot number: 20001102 Shanghai the 9th pharmaceutical factory produces.

2, experimental technique and result:

2.1 influence to tentative endometriosis model

2.1.1 the influence to rat endometrium dystopy model: the animal adaptability is raised a week back subcutaneous injection diethylstilbestrol 0.1ml/100g, once a day, injection is 3 days continuously, the 4th day with 3% pentobarbital sodium intraperitoneal injection of anesthesia, 0.1ml/100g, open abdomen, cut the uterus, left side and be about 2cm, the row end-to-end anastomosis, the endometrial tissue of peeling off is cut into the inner membrance piece of 3 * 3mm size with the slide calliper rule amount, be sewn on respectively right side cornua uteri, right side fallopian tube just in and near the right ovary, stitching thread is No. 6 nylon monofilament lines of not damaged, closes abdomen.Rat was divided into 8 groups at random in the 3rd after the moulding, be normal control group, model control group, positive drug control group and ZHG1 granule, ZHG2 granule, ZHG3 granule, ZHG4 granule, the ZHG5 groups of grains, 10 every group, the ZHG4 granule according to 9.0,4.5, the 2.25g/Kg gastric infusion, positive drug danazol capsule is according to the 67mg/Kg gastric infusion, successive administration 30 days, 31 days animal fasting 16h behind the medicine, pentobarbital sodium anesthesia, abdominal aortic blood is surveyed hemorheology index; Get uterus and ectopic tissue, perusal uterus and endometrial general form; 10% formaldehyde fixed, paraffin embedding, section, HE dyeing, high power microscope is observed endometrial tissue morphology down, adds up according to the grade scale of lesion degree, and compares between organizing; Under light microscopic, be unit with the little lattice number of eyepiece micrometer, count the number of false body of gland, measure the transverse diameter and the perpendicular footpath of false body of gland, the comparable group differences.

2.1.1.1 hemorheology result: the result shows that ZHG1 granule, ZHG2 granule, ZHG3 granule, ZHG4 granule, ZH65 granule all can reduce whole blood viscosity and the reduced viscosity (seeing Table 1,2) under the endometriosis model rat shear rate.Prompting ZHG granule has certain function of promoting blood circulation to disperse blood clots.

2.1.1.2 general form: uterus and endometrium perusal as seen: the organizational structure of rats in normal control group is normal, and not seeing has color change, and the uterus tube chamber does not see to have and increase or thicken that inner membrance is not seen hyperemia and blood stasis phenomenon.The endometrium of model group rat thickens, the inner membrance bright shape that takes on a red color, and there is cyst on surface, uterus, right side (dystopy endometrium side), cyst differs in size, the mutual fusion that has forms the Fructus Mori spline structure, part Ectopic Endometrium and adhesion on every side, and the individual animal adhesion is heavier.The dystopy uterus of positive drug and administration ZHG1 granule, ZHG2 granule, ZHG3 granule, ZHG4 granule, ZHG5 groups of grains rat is brown focus, endometrium thickens, inner membrance color and luster and symptoms such as inner membrance and its surrounding tissue adhesion all have than model group and alleviate to some extent, and wherein ZHG3 granule, the particulate change of ZHG4 are more obvious.

2.1.1.3 pathological tissue observed result (longitudinal section tissue morphology): the result as seen under the mirror: the endometrial epithelium of rats in normal control group is not seen hypertrophy, not seeing has inflammatory cell infiltration, and body of gland is not seen atrophy, and the full nothing of glandular epithelium comes off, and organizational structure is normal.The dystopy endometrium of model group rat has cyst to form, the cyst wall liner columnar epithelium of cyst, and the glandular epithelium like Ectopic Endometrium is atrophing state.The interstitial cell hyperplasia of superficial epithelium and inner membrance, part flesh layer thickens, Ectopic Endometrium is line simulator body sample and changes, mainly show as false glandular epithelium and be the atrophy shape, glandular sac chamber size is inhomogeneous, and form is irregular, matter has remote hemorrhage between the Ectopic Endometrium of individual animal, and a small amount of inflammatory cell infiltration is arranged, based on lymphocyte, and a small amount of oxyphil cell is arranged.Ectopic Endometrium cyst wall and the smooth muscle layer attenuation of each group (positive drug, ZHG1 granule, ZHG2 granule, ZHG3 granule, ZHG4 granule, ZHG5 groups of grains) rat of administration, false body of gland reduces, superficial epithelium and Interstitial cell rareness, wherein ZHG3 groups of grains, ZHG4 groups of grains Ectopic Endometrium cyst wall and the obvious attenuation of smooth muscle layer, the obvious atrophy of false body of gland, superficial epithelium and interstitial cell hyperplasia obviously alleviate, and add up according to grade scale (seeing Table 3 times).

Table 1, to influence (1) (X ± S of endometriosis hemorheology of rat; N=9)

Dosage whole blood viscosity (mpa/s ^-1)

Group

(g/Kg) 150s ^-1 38s ^-1 10s ^-1 5s ^-1

Normal 3.23 ± 0.45 4.62 ± 0.74 7.87 ± 1.33 11.34 ± 2.06

Model 4.94 ± 0.45 ^##7.15 ± 0.61 ^##12.61 ± 1.21 18.45 ± 1.96 ^##

Danazol 67mg 4.12 ± 0.25 ^*6.02 ± 0.40 ^*10.65 ± 0.87 ^*15.63 ± 1.44 ^*

ZHG4 4.5 3.65±0.27 ^** 5.35±0.53 ^** 9.49±1.32 ^** 13.95±2.25 ^**

ZHG2 4.5 3.96±0.50 ^** 5.70±0.71 ^** 9.88±1.34 ^** 14.35±2.09 ^**

ZHG1 4.5 4.26±0.27 ^** 6.34±0.49 ^** 11.17±1.15 ^**?16.56±1.67 ^*

ZHG3 4.5 4.01±0.23 ^** 5.98±0.44 ^** 10.09±1.20 ^**?15.38±2.11 ^**

ZHG5 4.5 3.87±0.19 ^** 5.66±0.41 ^** 9.46±1.16 ^** 14.59±1.43 ^**

Annotate: t check between group: compare with the normal control group: ##P＜0.01;

Compare with model control group: * P＜0.05; * P＜0.01

Table 2, to influence (2) (X ± S of metrecotpia hemorheology of rat; N=9)

Dosage reduced viscosity (mpa/s ^-1)

Group

(g/Kg) 150s ^-1 38s ^-1 10s ^-1 5s ^-1

Normal 5.09 ± 0.36 8.21 ± 0.50 15.67 ± 1.16 23.59 ± 2.03

Model 7.79 ± 0.95 ^##12.20 ± 1.18 ^##22.92 ± 1.90 ^##34.42 ± 2.88 ^##

Danazol 67mg 6.99 ± 0.67 ^*11.24 ± 0.92 ^*21.58 ± 1.72 ^*32.69 ± 2.71

ZHG4 4.5 5.37±0.67 ^** 8.46±1.17 ^** 17.20±3.01 ^** 26.24±5.05 ^**

ZHG2 4.5 6.58±1.34 ^* 10.45±2.03 ^* 19.78±3.93 ^* 29.65±6.08 ^*

ZHG1 4.5 6.56±0.47 ^** 10.56±0.63 ^** 20.30±1.29 ^** 30.77±2.20 ^*

ZHG3 4.5 6.29±1.2 ^* 10.41±1.99 ^* 18.88±4.03 ^* 29.20±6.12 ^*

ZHG5 4.5 6.47±1.35 ^* 10.06±1.28 ^* 19.43±3.55 ^* 29.11±6.30 ^*

Annotate: t check between group: compare with the normal control group: #P＜0.01; ##P＜0.01;

Compare with model control group: * P＜0.05; * P＜0.01.

Table 3, to the influence (n=10) of rat uterus Ectopic Endometrium histopathology

Group dosage (g/Kg) ++++++-

Normal 000 10

Model 6 ^#4 ^#0 ^#0 ^##

Danazol 67mg 0 ^*0 ^*4 ^*6 ^*

ZHG4 4.5 0 ^* 0 ^* 3 ^* 7 ^*

ZHG2 4.5 0 ^* 0 ^* 4 ^* 6 ^*

ZHG1 4.5 0 ^* 0 ^* 6 ^* 4 ^*

ZHG3 4.5 0 ^* 0 ^* 4 ^* 7 ^*

ZHG5 4.5 0 ^* 0 ^* 4 ^* 6 ^*

Annotate: X 2 test: compare with the normal control group: #P＜0.05; ##P＜0.01;

Compare with model control group: * P＜0.05; * P＜0.01

Grade scale: ++ the false body of gland of+dystopy endometrium is more, and cell infiltration and hemorrhagic focus are arranged.

++ the false body of gland of dystopy endometrium reduces cell infiltration and hemorrhage alleviating.

The basic atrophy of the false body of gland of+dystopy endometrium, cell infiltration and hemorrhagely obviously alleviate.

-dystopy endometrium is not seen false body of gland, does not see cell infiltration and hemorrhage.

Table 4, to the influence (X ± S of rat uterus Ectopic Endometrium body of gland; N=9)

False body of gland

Group dosage (g/Kg)

The perpendicular footpath of quantity (individual) transverse diameter

Normal 0--

Model 6.6 ± 3.9 ^##10.67 ± 2.48 ^##7.77 ± 1.25 ^##

Danazol 67mg 1.0 ± 0.9 ^*3.63 ± 2.33 ^*2.34 ± 1.56 ^*

ZHG4 4.5 0.6±0.7 ^** 2.00±2.20 ^** 1.06±1.27 ^**

ZHG2 4.5 1.2±0.8 ^** 2.68±1.83 ^** 1.63±1.38 ^**

ZHG1 4.5 1.9±0.7 ^** 4.15±1.12 ^** 3.41±0.98 ^**

ZHG3 4.5 0.7±0.7 ^** 2.13±2.16 ^** 1.05±1.26 ^**

ZHG5 4.5 1.7±0.8 ^** 2.99±1.95 ^** 3.15±1.09 ^**

Compare with model control group: * * P＜0.01

The unit in false body of gland transverse diameter and perpendicular footpath is the little lattice number (16 * 16) of micrometer under the light microscopic eyepiece

2.1.2 influence (test with ZHG4, below test together) to rabbit endometriosis model

Get the 2-2.5kg Female rabbits with 3% pentobarbital sodium intraperitoneal injection of anesthesia, lml/10kg opens abdomen, cuts a left side and then is about 3cm in the uterus, the row end-to-end anastomosis, physiological salt liquid is put in the uterus of downcutting rapidly, vertically cut open, flatten, be cut into 0.7 * 0.7cm size, be sewn on the inboard 1cm of vagina joint portion, uterus, right side, right side mesosalpinx and right ovary respectively, stitching thread is No. 6 nylon monofilament lines of not damaged, closes abdomen.Rat was divided into 6 groups at random in the 3rd after the moulding, it is the large, medium and small dosage group of normal control group, model control group, positive drug control group and ZHG4 granule, every group 8, the ZHG4 granule according to 3.0,1.5,0.75g/Kg dosage gastric infusion, positive drug danazol capsule is according to 20mg/Kg dosage gastric infusion, successive administration 30 days, 31 days animal fasting 16h behind the medicine, pentobarbital sodium anesthesia, abdominal aortic blood is surveyed hemorheology index; Get uterus and ectopic tissue, 10% formaldehyde fixed, paraffin embedding, section, histopathologic examination is done in HE dyeing, adds up according to the pathological grading standard, compares between organizing; Under light microscopic, be unit with the little lattice number of eyepiece micrometer, count the number of false body of gland, measure the transverse diameter and the perpendicular footpath of false body of gland, the comparable group differences.

2.1.2.1 hemorheological result:

The result shows that the large, medium and small dosage of ZHG4 granule all can reduce the whole blood reduced viscosity under the high, medium and low shear rate of endometriosis model rabbit; Dosage big or middle can reduce wherein, the whole blood viscosity (seeing Table 5,6) under the low shear rate.Prompting ZHG4 granule has certain function of promoting blood circulation to disperse blood clots.

2.1.2.2 general form: uterus and endometrium perusal as seen: normal control group rabbit uterus organizational structure is normal, and not seeing has color change, and the uterus tube chamber does not see to have and increase or thicken that inner membrance is not seen hyperemia and blood stasis phenomenon.The endometrium of model group rabbit thickens, the inner membrance bright shape that takes on a red color, and there is cyst on surface, uterus, right side (dystopy endometrium side), cyst differs in size, the mutual fusion that has forms the Fructus Mori spline structure, part Ectopic Endometrium and adhesion on every side, and the individual animal adhesion is heavier.The dystopy uterus of the large, medium and small dosage group of positive drug and administration rabbit is brown focus, and endometrium thickens, inner membrance color and luster and symptoms such as inner membrance and its surrounding tissue adhesion all have than model group and alleviate to some extent, and wherein the change of heavy dose of group is more obvious.

2.1.2.3 pathological tissue observed result (longitudinal section tissue morphology): the result as seen under the mirror: the endometrial epithelium of normal control group rabbit is not seen hypertrophy, not seeing has inflammatory cell infiltration, and body of gland is not seen atrophy, and the full nothing of glandular epithelium comes off, and organizational structure is normal.The dystopy endometrium of model group rabbit has cyst to form, and the cyst wall liner columnar epithelium of cyst is thinner, is atrophing state.The interstitial cell hyperplasia of superficial epithelium and inner membrance, most of flesh layer thickens, and Ectopic Endometrium is line simulator body sample and changes, mainly show as false glandular epithelium and be the atrophy shape, glandular sac chamber size is inhomogeneous, and form is irregular, matter has a small amount of inflammatory cell infiltration between the Ectopic Endometrium of individual animal, based on lymphocyte.Ectopic Endometrium cyst wall and the smooth muscle layer attenuation of each group (positive drug, large, medium and small dosage group) rabbit of administration, false body of gland reduces, superficial epithelium and Interstitial cell rareness, wherein heavy dose of group Ectopic Endometrium cyst wall and the obvious attenuation of smooth muscle layer, the obvious atrophy of false body of gland, superficial epithelium and interstitial cell hyperplasia obviously alleviate.Add up according to grade scale (seeing Table 3 times), present certain dose-effect relationship (seeing Table 7,8) between three dosage groups.

Table 5, to the rheol influence of metrecotpia rabbit blood (1) (X ± S; N=8)

Dosage whole blood viscosity (mpa/s ^-1)

Group

(g/Kg) 150s ^-1 38s ^-1 10s ^-1 5s ^-1

Normal 2.52 ± 0.35 3.62 ± 0.54 6.26 ± 1.09 9.07 ± 1.73

Model 3.60 ± 0.67 ^##5.51 ± 0.75 ^##10.33 ± 1.37 ^##15.83 ± 2.22 ^##

Danazol 67mg 2.73 ± 0.12 ^*3.87 ± 0.11 ^*6.59 ± 0.41 ^*9.48 ± 0.83 ^*

ZHG4 is big by 3.0 3.07 ± and 0.87 4.08 ± 0.83 ^*6.41 ± 2.00 ^*8.93 ± 3.73 ^*

Among the ZHG4 1.5 3.33 ± 0.43 4.29 ± 0.38 ^*7.04 ± 1.51 ^*11.36 ± 1.58 ^*

ZHG4 is little by 0.75 3.23 ± 0.62 4.70 ± 0.86 8.46 ± 2.04 12.16 ± 3.32 ^*

Annotate: t check between group: compare with the normal control group: #P＜0.05; ##P＜0.01;

Compare with model control group: * P＜0.05; * P＜0.01.

Table 6, to the rheol influence of metrecotpia rabbit blood (2) (X ± S; N=8)

Dosage reduced viscosity (mpa/s ^-1)

Group

(g/Kg) 150s ^-1 38s ^-1 10s ^-1 5s ^-1

Normal 4.45 ± 1.19 7.67 ± 1.96 15.20 ± 4.18 23.75 ± 6.42

Model 7.17 ± 1.78 ^##12.52 ± 2.37 ^##25.99 ± 5.23 ^##40.83 ± 9.25 ^##

Danazol 67mg 4.39 ± 0.42 ^*7.30 ± 0.78 ^*14.27 ± 2.11 ^*21.67 ± 3.68 ^*

ZHG4 3.0 4.97±0.90 ^** 7.24±1.46 ^** 12.44±3.98 ^** 18.17±7.60 ^**

Among the ZHG4 1.5 5.07 ± 0.89 ^*7.83 ± 0.93 ^*14.42 ± 3.32 ^*21.41 ± 6.36 ^*

ZHG4 is little by 0.75 5.08 ± 0.90 ^*8.45 ± 1.72 ^*16.71 ± 4.43 ^*25.60 ± 7.76 ^*

Annotate: T check between group: compare with the blank group: #P＜0.01; ##P＜0.01;

Compare with model group: *＜0.05; * P＜0.01.

Table 7, to the influence (n=8) of uterus Ectopic Endometrium histopathology

Group dosage (g/Kg) ++++++-

Normal 000 10

Model 3 ^#4 ^#10 ^##

Danazol 20mg 0 ^*1 ^*35 ^*

ZHG4 is big by 3.0 0 ^*0 ^*26 ^*

Among the ZHG4 1.5 0 ^*224 ^*

ZHG4 is little by 0.75 1223 ^*

Compare with model control group: * P＜0.05; * P＜0.01.

Grade scale: ++ the false body of gland of+dystopy endometrium is more, and the flesh layer is thicker, and has cell infiltration to reach

Hemorrhage.

The basic atrophy of the false body of gland of+dystopy endometrium, cell infiltration and hemorrhagely obviously subtract

Gently.

Table 8, to the influence (X ± S of rabbit uterus Ectopic Endometrium body of gland; N=8)

False body of gland

Group dosage (g/Kg)

The perpendicular footpath of quantity (individual) transverse diameter (mm) (mm)

Normal 0--

Model 13.4 ± 10.0 ^##8.51 ± 1.53 ^##4.59 ± 0.87 ^##

Danazol 20mg 1.5 ± 1.1 ^*4.74 ± 2.13 ^*2.59 ± 1.14 ^*

ZHG4 is big by 3.0 1.1 ± 0.8 ^*3.50 ± 2.25 ^*2.19 ± 1.46 ^*

Among the ZHG4 1.5 1.8 ± 1.0 ^*3.81 ± 1.69 ^*2.81 ± 1.11 ^*

ZHG4 is little by 0.75 2.5 ± 0.9 ^*5.04 ± 1.11 ^*3.45 ± 1.05 ^*

Compare with model control group: * * P＜0.01

2.2 influence to the nonspecific inflammation mould

2.2.1 influence (rat granuloma is swollen) to chronic inflammation model

Get body weight 150-180 gram male rat, adaptability is raised after 3 days with 3% pentobarbital sodium anesthesia (1.0ml/Kg), to weigh and to implant rat both sides pars inguinalis through autoclaved cotton balls subcutaneous, postoperative is divided into 5 groups at random, every group 10, perform the operation and began continuous gastric infusion 7 days the same day, the ZHG4 granule is big, middle low dose of group is respectively 9,3,1.5g/Kg the dosage of positive drug aspirin is 0.1g/Kg, postoperative was put to death animal on the 8th day, peel off and take out the cotton balls granulation tissue, weigh after 1 hour in 60-90 ℃ of baking oven inner drying, deduct the weight of cotton balls, be granulomatous clean amount, granulomatous weight between comparable group is calculated its suppression ratio.

The result shows that it is swollen that large, medium and small three dosage of ZHG4 granule all can suppress rat granuloma, prompting, and this product is to chronic non-different in nature inflammatory model inhibited (seeing Table 9).

Table 9, to the bullate influence (X ± S of rat granuloma; N=10)

Group dosage (g/Kg) granuloma dry weight mg suppression ratio %

Model 116.80 ± 15.48

Aspirin 0.1 74.90 ± 13.46 ^{* *}36

ZHG4 is big by 9 85.42 ± 13.18 ^{* *}27

Among the ZHG4 3 92.40 ± 20.19 ^*21

ZHG4 is little by 1.5 99.00 ± 17.44 ^*15

Annotate: the T check: compare with model control group: * P＜0.05; * P＜0.01; * * P＜0.001.

2.2.2 influence (dimethylbenzene mice ear) to the acute inflammation model

Getting body weight is 25-30 gram male mice, be divided into 5 groups at random, every group 10, gastric infusion is 3 days continuously, the ZHG4 granule is big, middle low dose of group is respectively 10,5,2.5gKg, the dosage of positive drug aspirin is 0.1g/Kg, and dimethylbenzene 0.1ml is dripped in the two sides, front and back of mice left side ear with sample injector, and auris dextra is contrast, put to death mice after 1 hour, cut two ears along the auricle baseline, lay round auricle at same position respectively, organize balance to weigh with the card punch of 8mm diameter, deduct the auris dextra sheet with the left ear of every Mus and heavily be the swelling degree, calculate the average and the standard deviation of the swelling degree of each group, calculate its suppression ratio, the comparable group differences.

The result shows that the big or middle dosage of ZHG4 granule all can suppress the ear swelling of mice caused by dimethylbenzene xylene, and onset dosage is 5,10g/Kg, and the effect of small dose group 2.5g/Kg is not obvious.Prompting this product has inhibitory action definitely to the non-specific acute inflammation model of animal.

Table 10, to the influence (X ± S of mice ear; N=10)

Group dosage (g/Kg) granuloma dry weight mg suppression ratio %

Model 26.7 ± 5.31

Aspirin 0.1 16.7 ± 5.50 ^*37

ZHG4 is big by 10 21.6 ± 5.34 ^*19

Among the ZHG4 5.0 22.1 ± 3.81 ^*17

ZHG4 is little by 2.5 24.5 ± and 4.60 8

Annotate: the T check: compare with model control group: * P＜0.05; * P＜0.01.

2.3 influence to non-specific pain model

2.3.1 chemical stimulation is caused the influence of pain model: getting body weight is the mice of 18-22 gram, be divided into 5 groups at random, every group 10, male and female half and half, gastric infusion is 3 days continuously, the big or middle low dose of group of ZHG4 granule is 10,5 respectively, 2.5g/Kg, the dosage of positive drug aspirin is 0.1g/Kg, the glacial acetic acid liquid 0.1ml/10g of the every Mus lumbar injection 0.6% of 0.5h after the not inferior administration, writhing response number of times behind the record injection acetic acid in the 20mim and incubation period of writhing response occurs, calculate the analgesia percentage rate, the comparable group differences.

The result shows that the big or middle dosage of ZHG4 granule all can suppress the pain reaction that glacial acetic acid causes mice, and large, medium and small three dosage all can prolong the incubation period that pain reaction appears in mice.Prompting this product has inhibitory action definitely to the non-specific acute pain model of animal.

Table 11, to the influence (X ± S of mouse writhing reaction; N=10)

Writhing response

Group dosage (g/Kg)

Suppression ratio %

Incubation period (S) number of times

Model 15.0 ± 4.1 14.3 ± 2.4

Aspirin 0.1 39.9 ± 12.6 ^*5.0 ± 1.8 ^*65

ZHG4 is big by 10.0 27.7 ± 7.6 ^*10.1 ± 2.9 ^*29

Among the ZHG4 5.0 23.9 ± 6.2 ^*11.2 ± 3.1 ^*22

ZHG4 is little by 2.5 21.3 ± 7.6 ^*12.3 ± 2.9 14

Annotate: T check between group: compare with model control group: *＜0.05; * P＜0.01.

2.3.2 thermostimulation is caused the influence of pain model: mice is placed on the homothermic thermostimulation of 55 degree causes on the pain instrument, drop into hot plate to the time that metapedes occurs licking from mice,, measure altogether 2 times, be no more than 30 seconds for qualified with meansigma methods as pain threshold with the stopwatch record.The qualified mice of learning from else's experience after screening is divided into 5 groups at random, every group 10, gastric infusion is 3 days continuously, the big or middle low dose of group of ZHG4 granule is 10,5 respectively, 2.5g/Kg, the dosage of positive drug aspirin is 0.1g/Kg, every interval 0.5h does not measure once after time administration, and METHOD FOR CONTINUOUS DETERMINATION to administration group mice does not have analgesic activity, surpasses 60 seconds in 60 seconds as pain threshold.Calculate the threshold of pain and improve percentage rate, comparable group differences.

Table 12, thermostimulation caused the influence (X ± S of mice pain reaction; N=10)

Pain threshold (S)

Group dosage (g/Kg)

0.5h 1h 1.5h 2h

Model 11.5 ± 2.9 14.0 ± 3.7 23.5 ± 11.9 17.5 ± 4.1

Aspirin 0.1 39.3 ± 15.4 ^*36.6 ± 14.8 ^*44.8 ± 17.8 ^*23.6 ± 6.4 ^*

ZHG4 is big by 10.0 29.4 ± 15.0 ^*27.5 ± 14.8 ^*33.0 ± 16.3 ^*21.5 ± 4.1 ^*

Among the ZHG4 5.0 25.1 ± 25.2 ^*24.8 ± 10.7 ^*33.2 ± 14.6 ^*19.9 ± 1.8

ZHG4 is little by 2.5 25.2 ± 12.3 ^*22.6 ± 10.2 ^*30.0 ± 13.86 ^*17.7 ± 6.2

Annotate: T check between group: compare with model control group: * P＜0.05; * P＜0.01.

3, conclusion

Two kinds of animal model changes aspect histopathology of rat and rabbit are close, and show as the symptom like the syndrome of blood stasis model, and promptly blood viscosity increases, and compare and know that the endometriosis model of rat is more successful down.Pharmacodynamics test shows that the ZHG4 granule can suppress the change of endometriosis rat and rabbit histopathology, reduces whole blood viscosity and reduced viscosity under its high, medium and low shear rate, and the effect of rat is better than rabbit; Suppress the swollen and mice dimethylbenzene ear swelling of rat granuloma; Suppress chemical stimulation and and thermostimulation cause the pain reaction of mice.The onset dosage of rabbit is 3,1.5,0.75g (crude drug)/Kg, and the onset dosage of rat is 9,4.5,2.25g (crude drug)/Kg, and the onset dosage of mice is 10,5,2.5g (crude drug)/Kg.Prompting ZHG4 granule has therapeutical effect to animal experiment temper endometriosis model, and has certain blood circulation promoting and blood stasis dispelling, analgesia and antiinflammatory action.

Experimental example 2 ZHG4 granules are originated to the pathologic finding report specimen of the influence of rat endometrium dystopy model: SD kind rat, and female.Censorship purpose: observe of the influence of ZHG4 granule to endometriosis rat model endometrium pathological tissue.Censorship internal organs: uterus.Experiment medicine: ZHG4 granule.Censorship number of animals: 60.The censorship date: on JIUYUE 27th, 2002.Pathology numbering: 19804-19863.Reporting date: on October 30th, 2002.

1, material and method: 1.1 material formaldehyde: Xu Dong chemical plant, Beijing produces (lot number: 20011023); Dehydrated alcohol: (lot number: 20011017), dimethylbenzene: (lot number: 2001040121), Yihong: (lot number: 810915) produce by the Beijing Chemical Plant; Lignum Sappan perfume (or spice): Beijing chemical reagents corporation produces (lot number: 940727); Paraffin: Shanghai China clever rehabilitation instrument plant produces (lot number 20020129) molten some 56-58 ℃; Resinene: Shanghai Sample Model Factory produces (lot number: 20000802).

1.2 instrument: Leitz rotary microtome: Germany produces; SAKURA automatic dehydration machine, SAKURARSH-100 automatic staining machine, NiKon optical microscope, the biological microphotograph system automatically of OLYMPUS BH-2 produce by Japan.

1.3 method: 60 of female rats are divided into 6 groups of the large, medium and small dosage groups of normal control group, model group, positive drug group and administration, 10 every group, dissect behind the sacrifice of animal, getting ectopic uterus, to put into 10% formalin fixing, the back flowing water flushing of drawing materials, gradient ethanol (60,70,80,90,95,100%) dehydration, dimethylbenzene is transparent, the waxdip embedding, section, HE dyeing, resinene glue mounting, its tissue morphology of observation by light microscope.

Pathology numbering: 10 of matched groups: 19804-19813; 10 of model group: 19814-19823; 10 of positive drug groups: 19824-19833; 10 of heavy dose of groups: 19834-19943; 10 of middle dosage groups: 19844-19853; 10 of small dose group: 19854-19863.

2, result: 2.1 perusal results

The normal control group: organizational structure is normal, and not seeing has color change, and the uterus tube chamber does not see to have and increase or thicken that inner membrance is not seen congested machine blood stasis phenomenon.

Model group: endometrium thickens, the inner membrance bright shape that takes on a red color, and there is cyst on surface, uterus, right side, and cyst differs in size, and the mutual fusion that has forms the Fructus Mori spline structure, part Ectopic Endometrium and adhesion on every side, the individual animal adhesion is heavier.

The large, medium and small dosage group of positive drug and administration: the dystopy uterus of animal is brown focus, endometrium thickens, inner membrance color and luster and inner membrance and its surrounding tissue adhesion all have to some extent than the model group animal and alleviate, and wherein the change of heavy dose of group is more obvious.

2.2 observed result under the mirror (longitudinal section tissue morphology): the normal control group: endometrial epithelium is not seen hypertrophy, and not seeing has inflammatory cell infiltration, and body of gland is not seen atrophy, the full nothing of glandular epithelium comes off, and organizational structure is normal.Model group: the dystopy endometrium has cyst to form, the cyst wall liner columnar epithelium of cyst, and the glandular epithelium like Ectopic Endometrium is atrophing state.The interstitial cell hyperplasia of superficial epithelium and inner membrance, part flesh layer thickens, Ectopic Endometrium is line simulator body sample and changes, mainly show as false glandular epithelium and be the atrophy shape, glandular sac chamber size is inhomogeneous, and form is irregular, matter has remote hemorrhage between the Ectopic Endometrium of individual animal, and a small amount of inflammatory cell infiltration is arranged, based on lymphocyte, and a small amount of oxyphil cell is arranged.Administration group (positive drug, large, medium and small dosage group): Ectopic Endometrium cyst wall and smooth muscle layer attenuation, false body of gland reduces, superficial epithelium and Interstitial cell rareness, wherein heavy dose of group Ectopic Endometrium cyst wall and the obvious attenuation of smooth muscle layer, the obvious atrophy of false body of gland, superficial epithelium and interstitial cell hyperplasia obviously alleviate.

3, conclusion: matched group body of gland size uniformity, quantity is more.Model group dystopy endometrial gland size is inhomogeneous, and form is irregular, and the glandular epithelium atrophy forms false body of gland.The pathologic that positive drug and the obvious atrophy of administration three dosage group Ectopic Endometrium bodies of gland, demonstration can suppress dystopy endometriosis rat to some extent changes.

Experimental example 3 ZHG4 granules influence the pathologic finding report to the endometriosis model of animal

The specimen source: new zealand rabbit, female.Censorship purpose: observe of the influence of ZHG4 granule to endometriosis animal pattern endometrium pathological tissue.Censorship internal organs: uterus.Experiment medicine: ZHG4 granule.Censorship number of animals: 48.The censorship date: on October 25th, 2002.Pathology numbering: 20221-20268.Reporting date: on December 10th, 2002.

2, material and method: 2.1 materials: formaldehyde: Xu Dong chemical plant, Beijing produces (lot number: 20011023); Dehydrated alcohol: (lot number: 20011017), dimethylbenzene: (lot number: 2001040121), Yihong: (lot number: 810915) produce by the Beijing Chemical Plant; Lignum Sappan perfume (or spice): Beijing chemical reagents corporation produces (lot number: 940727); Paraffin: Shanghai China clever rehabilitation instrument plant produces (lot number 20020129) molten some 56-58 ℃; Resinene: Shanghai Sample Model Factory produces (lot number: 20000802).2.2 instrument: Leitz rotary microtome: Germany produces: SAKURA automatic dehydration machine, SAKURA RSH-100 automatic staining machine, NiKon optical microscope, the biological microphotograph system automatically of OLYMPUS BH-2 produce by Japan.2.3 method: 48 of female rabbits are divided into 6 groups of the large, medium and small dosage groups of normal control group, model group, positive drug group and administration, 8 every group, dissect behind the sacrifice of animal, getting ectopic uterus, to put into 10% formalin fixing, the back flowing water flushing of drawing materials, gradient ethanol (60,70,80,90,95,100%) dehydration, dimethylbenzene is transparent, the waxdip embedding, section, HE dyeing, resinene glue mounting, its tissue morphology of observation by light microscope.

Pathology numbering: 8 of matched groups: 20221-20228; 8 of model group: 20229-20236; 8 of positive drug groups: 20237-20244; 8 of heavy dose of groups: 20245-20252; 8 of middle dosage groups: 20253-20260; 8 of small dose group: 20261-20268.：

3, result

3.1 perusal result: the normal control group: organizational structure is normal, and not seeing has color change, and the uterus tube chamber does not see to have and increase or thicken that inner membrance is not seen congested machine blood stasis phenomenon.Model group: endometrium thickens, the inner membrance bright shape that takes on a red color, and there is cyst on surface, uterus, right side, and cyst differs in size, and the mutual fusion that has forms the Fructus Mori spline structure, part Ectopic Endometrium and adhesion on every side, the individual animal adhesion is heavier.The large, medium and small dosage group of positive drug and administration: the dystopy uterus of animal is brown focus, and endometrium thickens, inner membrance color and luster and inner membrance and its surrounding tissue adhesion all have to some extent than model and alleviate, and wherein the change of heavy dose of group is more obvious.

3.2 observed result under the mirror (longitudinal section tissue morphology): the normal control group: endometrial epithelium is not seen hypertrophy, and not seeing has inflammatory cell infiltration, and body of gland is not seen atrophy, the full nothing of glandular epithelium comes off, and organizational structure is normal.Model group: the dystopy endometrium has cyst to form, the cyst wall liner columnar epithelium of cyst, and the glandular epithelium like Ectopic Endometrium is atrophing state.The interstitial cell hyperplasia of superficial epithelium and inner membrance, part flesh layer thickens, Ectopic Endometrium is line simulator body sample and changes, mainly show as false glandular epithelium and be the atrophy shape, glandular sac chamber size is inhomogeneous, and form is irregular, matter has remote hemorrhage between the Ectopic Endometrium of individual animal, and a small amount of inflammatory cell infiltration is arranged, based on lymphocyte, and a small amount of oxyphil cell is arranged.Administration group (positive drug, big, mountain, small dose group): Ectopic Endometrium cyst wall and smooth muscle layer attenuation, false body of gland reduces, superficial epithelium and Interstitial cell rareness, wherein heavy dose of group Ectopic Endometrium cyst wall and the obvious attenuation of smooth muscle layer, the obvious atrophy of false body of gland, superficial epithelium and interstitial cell hyperplasia obviously alleviate.

4, conclusion

Matched group body of gland size uniformity, quantity is more.Model group dystopy endometrial gland size is inhomogeneous, and form is irregular, and the glandular epithelium atrophy forms false body of gland.Positive drug and the obvious atrophy of administration three dosage group Ectopic Endometrium bodies of gland, demonstration can suppress the endometrial pathologic of model group dystopy to some extent and change.

Following embodiment all can realize the effect of above-mentioned experimental example.

Embodiment 1Capsule

Oletum Trogopterori 200g Radix Linderae 166.67g Radix Angelicae Sinensis 166.67g

Radix Paeoniae Rubra 166.67g Herba Leonuri 166.67g Myrrha 166.67g

Pheretima 100g Spina Gleditsiae 266.67g

More than eight flavors, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amount alcohol heating reflux of 70% and extract secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1.,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Herba Leonuri adds 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Herba Leonuri medicinal residues and Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Angelicae Sinensis adds 6 times of water gagings, soaks 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind the Radix Angelicae Sinensis extraction volatile oil add the ethanol of 8 times of amounts 95%, reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and gets the ZHG1 granule, or encapsulated, gets 1000 of ZHG1 capsules.

Embodiment 2Tablet

Oletum Trogopterori 210g Radix Linderae 170g Radix Angelicae Sinensis 170g

Radix Paeoniae Rubra 170g Herba Leonuri 170g Myrrha 180g

Pheretima 90g Spina Gleditsiae 250g

More than eight flavors, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amount alcohol heating reflux of 70% and extract secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1.,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Herba Leonuri adds 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Herba Leonuri medicinal residues and Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Angelicae Sinensis adds 6 times of water gagings, soaks 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind the Radix Angelicae Sinensis extraction volatile oil add the ethanol of 8 times of amounts 95%, reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; Add the 34%-36% dextrin and the volatile oil clathrate compound of granule theoretical yield in the dry powder, mixing makes 1000 through conventional technology.

Embodiment 3Capsule

Ramulus Cinnamomi 166.67g Radix Aconiti Lateralis Preparata (system) 166.67g Radix Salviae Miltiorrhizae 416.67g

Rhizoma Corydalis 166.67g Flos Carthami 200g Radix Paeoniae Alba 100g

Radix Paeoniae Rubra 166.67g Herba Leonuri 166.67g Myrrha 166.67g

Pheretima 100g Spina Gleditsiae 266.67g

More than 14 the flavor, the alcohol heating reflux that Rhizoma Corydalis, the Radix Paeoniae Alba, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Ramulus Cinnamomi, Radix Angelicae Sinensis, the Flos Carthami extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of ZHG2 capsules.Take every day three times, be equivalent to crude drug 10.5g at every turn.

Embodiment 4Granule

Ramulus Cinnamomi 180g Radix Aconiti Lateralis Preparata (system) 150g Radix Salviae Miltiorrhizae 430g

Rhizoma Corydalis 180g Flos Carthami 190g Radix Paeoniae Alba 120g

Oletum Trogopterori 220g Radix Linderae 150g Radix Angelicae Sinensis 150g

Radix Paeoniae Rubra 180g Herba Leonuri 180g Myrrha 185g

Pheretima 110g Spina Gleditsiae 245g

More than 14 the flavor, the alcohol heating reflux that Rhizoma Corydalis, the Radix Paeoniae Alba, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Ramulus Cinnamomi, Radix Angelicae Sinensis, the Flos Carthami extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and gets granule.Packing is promptly taken three times every day, is equivalent to crude drug 10.5g at every turn.

Embodiment 5Capsule

Cortex Cinnamomi 166.67g Radix Aconiti (system) 166.67g Cortex Moutan 416.67g

Lignum Sappan 166.67g Flos Carthami 200g rhizoma sparganic 100g

Radix Paeoniae Rubra 166.67g Herba Leonuri 166.67g Myrrha 166.67g

Pheretima 100g Spina Gleditsiae 266.67g dextrin is an amount of

More than 14 the flavor, the alcohol heating reflux that Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri medicinal residues and Radix Aconiti Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Cortex Cinnamomi, Radix Angelicae Sinensis, the Flos Carthami extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of ZHG3 capsules.

Embodiment 6Capsule

Lignum Sappan 166.67g Rhizoma Curcumae 200g rhizoma sparganic 100g

Radix Paeoniae Rubra 166.67g Herba Leonuri 166.67g Myrrha 166.67g

Pheretima 100g Spina Gleditsiae 266.67g dextrin is an amount of

More than 14 the flavor, the alcohol heating reflux that Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Ramulus Cinnamomi, Radix Angelicae Sinensis, the Rhizoma Curcumae extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of ZHG4 capsules.

Embodiment 7Oral liquid

Ramulus Cinnamomi 150g Radix Aconiti Lateralis Preparata (system) 170g Radix Salviae Miltiorrhizae 430g

Lignum Sappan 180g Rhizoma Curcumae 220g rhizoma sparganic 120g

Oletum Trogopterori 180g Radix Linderae 145g Radix Angelicae Sinensis 145g

Radix Paeoniae Rubra 185g Herba Leonuri 185g Myrrha 185g

Pheretima 110g Spina Gleditsiae 275g

More than 14 the flavor, the alcohol heating reflux that Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Ramulus Cinnamomi, Radix Angelicae Sinensis, the Rhizoma Curcumae extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, add water to 2500ml; Above-mentioned volatile oil adds the 60-80ml tween 80, and the dissolving back adds in the medicinal liquid, stirs, promptly.Every bottle of 10ml takes three every day, is equivalent to crude drug 10.5g at every turn.

Embodiment 8Capsule

Cortex Cinnamomi 166.67g Radix Aconiti Lateralis Preparata (system) 166.67g Radix Salviae Miltiorrhizae 416.67g

Rhizoma Corydalis 166.67g Rhizoma Curcumae 200g rhizoma sparganic 100g

Radix Paeoniae Rubra 166.67g Herba Leonuri 166.67g Myrrha 166.67g

Pheretima 100g Spina Gleditsiae 266.67g dextrin is an amount of

More than 14 the flavor, the alcohol heating reflux that Rhizoma Corydalis, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Cortex Cinnamomi, Radix Angelicae Sinensis, the Rhizoma Curcumae extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary each 1.5 hours, filters, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ to comprise in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets the volatile oil inclusion; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of ZHG5 capsules.Take every day three times, 3～4 of every days, be equivalent to crude drug 10.5g at every turn.

Claims

1, a kind of Chinese medicine compositions for the treatment of endometriosis is characterized in that the crude drug of this pharmaceutical composition comprises following raw material medicaments:

Oletum Trogopterori 170-230 weight portion Radix Linderae 135-190 weight portion

Radix Angelicae Sinensis 135-190 weight portion Radix Paeoniae Rubra 135-190 weight portion

Herba Leonuri 135-190 weight portion Myrrha 135-190 weight portion

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion.

2, pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:

The Oletum Trogopterori 200 weight portion Radixs Linderae 166.67 weight portions

Radix Angelicae Sinensis 166.67 weight portion Radix Paeoniae Rubra 166.67 weight portions

Herba Leonuri 166.67 weight portion Myrrhas 166.67 weight portions

Pheretima 100 weight portion Spina Gleditsiaes 266.67 weight portions.

3, pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Herba Leonuri 135-190 weight portion Myrrha 135-190 weight portion

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion

Ramulus Cinnamomi 135-190 weight portion Radix Aconiti Lateralis Preparata 135-190 weight portion

Radix Salviae Miltiorrhizae 390-440 weight portion Rhizoma Corydalis 135-190 weight portion

Flos Carthami 180-220 weight portion Radix Paeoniae Alba 80-120 weight portion.

4, pharmaceutical composition as claimed in claim 3 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Ramulus Cinnamomi 166.67 weight portion Radix Aconiti Lateralis Preparatas 166.67 weight portions

Radix Salviae Miltiorrhizae 416.67 weight portion Rhizoma Corydalis 166.67 weight portions

The Flos Carthami 200 weight portion Radix Paeoniae Albas 100 weight portions

Herba Leonuri 166.67 weight portion Myrrhas 166.67 weight portions

Pheretima 100 weight portion Spina Gleditsiaes 266.67 weight portions.

5, pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Herba Leonuri 135-190 weight portion Myrrha 135-190 weight portion

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion

Cortex Cinnamomi 135-190 weight portion Radix Aconiti 135-190 weight portion

Cortex Moutan 416.67 weight portion Lignum Sappan 135-190 weight portions

Flos Carthami 180-220 weight portion rhizoma sparganic 80-120 weight portion.

6, pharmaceutical composition as claimed in claim 5 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Cortex Cinnamomi 166.67 weight portion Radix Aconitis (system) 166.67 weight portions

Cortex Moutan 416.67 weight portion Lignum Sappans 166.67 weight portions

Flos Carthami 200 weight portion rhizoma sparganic 100 weight portions

Herba Leonuri 166.67 weight portion Myrrhas 166.67 weight portions

Pheretima 100 weight portion Spina Gleditsiaes 266.67 weight portions.

7, pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Herba Leonuri 135-190 weight portion Myrrha 135-190 weight portion

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion

Radix Salviae Miltiorrhizae 390-440 weight portion Lignum Sappan 135-190 weight portion

8, pharmaceutical composition as claimed in claim 7 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Herba Leonuri 166.67 weight portion Myrrhas 166.67 weight portions

Pheretima 100 weight portion Spina Gleditsiaes 266.67 weight portions

Radix Salviae Miltiorrhizae 416.67 weight portion Lignum Sappans 166.67 weight portions

Rhizoma Curcumae 200 weight portion rhizoma sparganic 100 weight portions.

9, pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Herba Leonuri 135-190 weight portion Myrrha 135-190 weight portion

Pheretima 80-120 weight portion Spina Gleditsiae 235-290 weight portion

Cortex Cinnamomi 135-190 weight portion Radix Aconiti Lateralis Preparata 135-190 weight portion

10, pharmaceutical composition as claimed in claim 9 is characterized in that this pharmaceutical composition made by following raw material medicaments:

Cortex Cinnamomi 166.67 weight portion Radix Aconiti Lateralis Preparatas 166.67 weight portions

Rhizoma Curcumae 200 weight portion rhizoma sparganic 100 weight portions

Herba Leonuri 166.67 weight portion Myrrhas 166.67 weight portions

Pheretima 100 weight portion Spina Gleditsiaes 266.67 weight portions.

11,, it is characterized in that the preferred Radix Aconiti Lateralis Preparata of Radix Aconiti Lateralis Preparata in this pharmaceutical composition as claim 3 or 4 described pharmaceutical compositions.

12,, it is characterized in that the preferred Radix Aconiti Lateralis Preparata of Radix Aconiti Lateralis Preparata in this pharmaceutical composition as claim 7 or 8 described pharmaceutical compositions.

13,, it is characterized in that the preferred Radix Aconiti Preparata of Radix Aconiti in this pharmaceutical composition as claim 5 or 6 described pharmaceutical compositions.

14,, it is characterized in that the preferred Radix Aconiti Lateralis Preparata of Radix Aconiti Lateralis Preparata in this pharmaceutical composition as claim 9 or 10 described pharmaceutical compositions.

15,, it is characterized in that this pharmaceutical composition can add excipient and make clinical acceptable forms, as pill, tablet, granule, oral liquid, capsule, masticatory as the described pharmaceutical composition of right 1-10.

16, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 1 or 2, it is characterized in that this method is: the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 8-12 doubly measures 60-80% and extract 1-3 time, each 1-3 hour, filter, merge filtrate twice, get filtrate 1. ,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Herba Leonuri adds 5-7 and doubly measures 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, merge filtrate twice, get filtrate 2.,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Herba Leonuri medicinal residues and Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merge three times filtrate, filtrate 3., the clear paste that to be concentrated into 80-90 ℃ of density be 1.08-1.10 adds ethanol and makes and contain the alcohol amount and reach 60-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, continue to concentrate the 80-90 ℃ of clear paste that density is 1.08-1.10; Radix Angelicae Sinensis adds 5-7 times of water gaging, soaked 60 minutes, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, filtrate 4., 4000-6000r/min is centrifugal, gets supernatant, the clear paste that to be concentrated into 80-90 ℃ of density be 1.08-1.10; The medicinal residues that Radix Angelicae Sinensis extracts behind the volatile oil add 8 times of ethanol of measuring 85-95%, reflux, extract, 2-3 time, each 1-2 hour, filter, merge filtrate twice, get filtrate 5., filtrate--0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 5-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and gets capsule.

17, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 16, it is characterized in that this method is: the alcohol heating reflux that the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Herba Leonuri adds 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Herba Leonuri medicinal residues and Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Angelicae Sinensis adds 6 times of water gagings, soaks 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind the Radix Angelicae Sinensis extraction volatile oil add the ethanol of 8 times of amounts 95%, reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and gets 1000 of capsules.

18, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 11, it is characterized in that this method is: Rhizoma Corydalis, the Radix Paeoniae Alba, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 8-12 doubly measures 60-80% and extract 1-3 time, each 1-3.0 hour, filter, 1. merging filtrate gets filtrate ,-0.09～-0.066Mpa, 70-85 ℃ of decompression recycling ethanol continues to be evaporated to 80-90 ℃ of density the clear paste that is 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 60-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time, each 1-2 hour, filter, 5. merging filtrate gets filtrate, filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 5-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets capsule.

19, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 18, it is characterized in that this method is: the alcohol heating reflux that Rhizoma Corydalis, the Radix Paeoniae Alba, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Ramulus Cinnamomi, Radix Angelicae Sinensis, the Flos Carthami extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of capsules.

20, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 13, it is characterized in that this method is: Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 8-12 doubly measures 65-80% and extract 1-3 time, each 1-3 hour, filter, 1. merging filtrate gets filtrate ,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri add 6-7 and doubly measure 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri medicinal residues and Radix Aconiti Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 65-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Cortex Cinnamomi, Radix Angelicae Sinensis, Flos Carthami extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time, each 1-2 hour, filter, 5. merging filtrate gets filtrate, filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 5-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets capsule.

21, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 20, it is characterized in that this method is: the alcohol heating reflux that Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Moutan, Herba Leonuri medicinal residues and Radix Aconiti Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, continuing to concentrate 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Flos Carthami add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Cortex Cinnamomi, Radix Angelicae Sinensis, the Flos Carthami extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of capsules.

22, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 12, it is characterized in that this method is: Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 9-11 doubly measures 60-75% and extract 1-3 time, each 1-3 hour, filter, 1. merging filtrate gets filtrate ,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 5-7 and doubly measure 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 65-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time, each 1-2 hour, filter, 5. merging filtrate gets filtrate, filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 5-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets capsule.

23, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 22, it is characterized in that this method is: the alcohol heating reflux that Lignum Sappan, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Ramulus Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Ramulus Cinnamomi, Radix Angelicae Sinensis, the Rhizoma Curcumae extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary, each 1.5 hours, filter, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Merge each clear paste, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ and carries out enclose in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; Yu Fenzhong adds the 34%-36% dextrin and the volatile oil clathrate compound of granule theoretical yield, and mixing is granulated, and is encapsulated, gets 1000 of capsules.

24, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 14, it is characterized in that this method is: Rhizoma Corydalis, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add the alcohol heating reflux that 9-11 doubly measures 65-80% and extract 1-3 time, each 1-3 hour, filter, 1. merging filtrate gets filtrate ,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 5-7 and doubly measure 85-95% ethanol, heating and refluxing extraction 1-3 time, each 1-2 hour, filter, 2. merging filtrate gets filtrate,-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water that 5-7 doubly measures, decoct 2-4 time, each 2-4 hour, filter, merging filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes the alcohol amount of containing reach 65-80%, left standstill 22-26 hour, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 5-7 times of water gaging, soaked 50-70 minute, and steam distillation 5-7 hour, extract volatile oil with oil water separator, standby; Extracting solution filters, and gets filtrate 4., and 4000-6000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; The medicinal residues that Cortex Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae extract behind the volatile oil add the ethanol that 7-9 doubly measures 85-95%, reflux, extract, 1-3 time each 1-2 hour, filters, merging filtrate, get filtrate 5., filtrate-0.09～-0.06Mpa, 70-85 ℃ of decompression recycling ethanol, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1 with the ratio of volatile oil and beta-schardinger dextrin-: 5-7, and beta-schardinger dextrin-is 1 with the ratio of water: 1-3, adopt paddling process, carry out enclose in 1-3 hour in 50-70 ℃ of stirring, filter, get pastel, 30-50 ℃ of drying pulverized, and promptly gets volatile oil clathrate compound; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets capsule.

25, the capsule preparation method thereof of pharmaceutical composition as claimed in claim 24, it is characterized in that this method is: the alcohol heating reflux that Rhizoma Corydalis, rhizoma sparganic, the Radix Linderae, Spina Gleditsiae, Radix Paeoniae Rubra add 10 times of amounts 70% extracts secondary, each 2.0 hours, filter, merge filtrate twice, get filtrate 1. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri add 6 times of amount 90% ethanol, the heating and refluxing extraction secondary, and each 1 hour, filter, merge filtrate twice, get filtrate 2. ,-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Radix Salviae Miltiorrhizae, Herba Leonuri medicinal residues and Radix Aconiti Lateralis Preparata, Oletum Trogopterori, Myrrha, Pheretima add the water of 6 times of amounts, decoct three times, each 3 hours, filter, merge three times filtrate, filtrate 3., being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, adds ethanol and makes and contain alcohol amount and reach 70%, left standstill 24 hours, get supernatant, reclaim ethanol, 80 ℃-90 ℃ are continued to concentrate density is the clear paste of 1.08-1.10; Cortex Cinnamomi, Radix Angelicae Sinensis, Rhizoma Curcumae add 6 times of water gagings, soak 60 minutes, and steam distillation 6.0 hours extracts volatile oil with oil water separator, and is standby; Extracting solution filters, and gets filtrate 4., and 5000r/min is centrifugal, gets supernatant, and being concentrated into 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10; Medicinal residues behind Cortex Cinnamomi, Radix Angelicae Sinensis, the Rhizoma Curcumae extraction volatile oil add the ethanol of 8 times of amounts 95%, the reflux, extract, secondary each 1.5 hours, filters, merge filtrate twice, get filtrate 5., filtrate-0.07Mpa, 80 ℃ of decompression recycling ethanols, continuing to be evaporated to 80 ℃ of-90 ℃ of density is the clear paste of 1.08-1.10, mixing, spray drying gets dry powder; Volatile oil adds an amount of dissolve with ethanol, is 1: 6 with the ratio of volatile oil and beta-schardinger dextrin-, and beta-schardinger dextrin-is 1: 2 with the ratio of water, adopts paddling process, stirs in 60 ℃ to comprise in 2 hours, filter, pastel, 30-40 ℃ of drying pulverized, and promptly gets the volatile oil inclusion; The 34%-36% dextrin and the volatile oil clathrate compound that add the granule theoretical yield in the dry powder, mixing is granulated, and is encapsulated, gets 1000 of capsules.