CN1546534A - Method for extraction and purification of polysaccharide from pilose antler - Google Patents
Method for extraction and purification of polysaccharide from pilose antler Download PDFInfo
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- CN1546534A CN1546534A CNA2003101159233A CN200310115923A CN1546534A CN 1546534 A CN1546534 A CN 1546534A CN A2003101159233 A CNA2003101159233 A CN A2003101159233A CN 200310115923 A CN200310115923 A CN 200310115923A CN 1546534 A CN1546534 A CN 1546534A
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- pilose antler
- weight
- aqueous solution
- polysaccharide
- active polysaccharide
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Abstract
The invention provides a method for extraction and purification of polysaccharide from pilose antler which comprises the steps of, cornucervi pantotrichum water immersion, cytoclasis, grinding through colloid mill, multiple freezing, enzymatic hydrolyzing, repeated depositing. The prepared cornucervi pantotrichum polysaccharide not only possesses the biological functions of other polysaccharides, but also contains growth factor.
Description
Technical field
The present invention relates to a kind of from animal product, extract the purifying active polysaccharide (Pilose AntlerPolsacchrides, be called for short: method PAPS), more specifically say so and from pilose antler, extract the method for purifying active polysaccharide.
Background technology
At present, the active polysaccharide of data at home and abroad report all prepares by zymotechnique from natural phant and fungi, for example: lentinan etc.From pilose antler, extract active polysaccharide and also do not see report.The pilose antler active polysaccharide is compared with other polysaccharide, except that the biological function with other active polysaccharide, also has the unique biological activity, and sulfuric acid pilose antler chrondroitin is one of its main component, and it also contains somatomedin simultaneously.Their energy promote osteogenesis, wound healing and treatment tumour.
Summary of the invention
The purpose of this invention is to provide a kind of method of extracting the purifying active polysaccharide from pilose antler, this method comprises the step of following order:
(1) added the water logging bubble 6-8 hour with the pilose antler meal with 1: 1 (weight) ratio, cytoclasis with the further homogenate of colloidal mill, is made the pilose antler aqueous solution;
(2) the pilose antler aqueous solution after making adds water move to the aqueous solution that dry matter content is 25% (weight), melts multigelation after being refrigerated to-20 ℃;
(3) with the pilose antler aqueous solution, under the effect of 1.5 ‰ (weight) papain, pH value is 6.0～8.0,60～65 ℃ of temperature, hydrolysis 24～30 hours;
(4) precipitation of the pilose antler aqueous solution after the hydrolysis is removed impurity, get the trichoroacetic acid(TCA) that supernatant liquor adds 7% (weight), make protein denaturation;
(5) add 95% ethanol by 1: 25 (weight) again and make its sedimentation, supernatant solution adds the ethanol sedimentation again, repeated multiple times, and the collecting precipitation thing promptly gets the pilose antler Crude polysaccharides;
(6) gained throw out, refining with existing purification process, promptly get purifying pilose antler active polysaccharide.
Embodiment
Get the pilose antler meal and added the water logging bubble 6-8 hour with 1: 1 (weight) ratio, cytoclasis with the further homogenate of colloidal mill, is made the pilose antler aqueous solution; Add water move to the aqueous solution that dry matter content is 25% (weight), melt multigelation after being refrigerated to-20 ℃; With the pilose antler aqueous solution, under the effect of 1.5 ‰ (weight) papain, pH value is controlled at 6.0～8.0,60～65 ℃ of temperature, hydrolysis 24～30 hours; Precipitation is removed impurity, gets the trichoroacetic acid(TCA) that supernatant liquor adds 7% (weight), makes protein denaturation; Add 95% ethanol by 1: 25 (weight) again and make its sedimentation, supernatant solution adds the ethanol sedimentation again, repeated multiple times, and the collecting precipitation thing promptly gets the pilose antler Crude polysaccharides; The gained throw out, refining with existing purification process, promptly get purifying pilose antler active polysaccharide.
Claims (1)
1. method of extracting purifying pilose antler active polysaccharide from pilose antler, this method comprises the steps: in regular turn
(1) add entry with the pilose antler meal with 1: 1 (weight) ratio, cytoclasis with the further homogenate of colloidal mill, is made the pilose antler aqueous solution;
(2) to add water move to dry matter content be 25% (weight) aqueous solution to the pilose antler aqueous solution after making, and melts multigelation after being refrigerated to-20 ℃;
(3) with the pilose antler aqueous solution, under the effect of 1.5 ‰ (weight) papain, pH value is 6.0～8.0,60～65 ℃ of temperature, hydrolysis 24～30 hours;
(4) precipitation of the pilose antler aqueous solution after the hydrolysis is removed impurity, get the trichoroacetic acid(TCA) that supernatant liquor adds 7% (weight), make protein denaturation;
(5) add 95% ethanol by 1: 25 (weight) again and make its sedimentation, supernatant solution adds the ethanol sedimentation again, repeated multiple times, and the collecting precipitation thing promptly gets the pilose antler Crude polysaccharides;
(6) gained throw out, refining with existing purification process, promptly get purifying pilose antler active polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101159233A CN1300324C (en) | 2003-12-12 | 2003-12-12 | Method for extraction and purification of polysaccharide from pilose antler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101159233A CN1300324C (en) | 2003-12-12 | 2003-12-12 | Method for extraction and purification of polysaccharide from pilose antler |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1546534A true CN1546534A (en) | 2004-11-17 |
| CN1300324C CN1300324C (en) | 2007-02-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101159233A Expired - Fee Related CN1300324C (en) | 2003-12-12 | 2003-12-12 | Method for extraction and purification of polysaccharide from pilose antler |
Country Status (1)
| Country | Link |
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| CN (1) | CN1300324C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642466B (en) * | 2009-05-12 | 2013-01-30 | 王振宇 | Preparation method of composition containing pilose antler polysaccharides and having function of reducing blood lipids |
| CN102987501A (en) * | 2012-10-17 | 2013-03-27 | 徐州鸿宇农业科技有限公司 | Method for preparing nutrient (liquid) beverages and granules of healthcare food (therapy) products by using extractives of cordyceps militaris and cocoons |
| CN106432530A (en) * | 2016-11-24 | 2017-02-22 | 福建省农业科学院土壤肥料研究所 | Extracting technology of dictyophora indusiata volva colloid polysaccharide |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1111171C (en) * | 2000-09-07 | 2003-06-11 | 上海惠海生化制品厂 | Heparin and its preparing process |
| CN1193770C (en) * | 2003-01-09 | 2005-03-23 | 长春市双阳区鹿业科学技术研究所 | Hydrolytic extrating method for medicinal component of pilose antler |
| CN1228347C (en) * | 2003-01-16 | 2005-11-23 | 上海寿祺多糖食品研究所 | Process for extracting lichee polysaccharide |
-
2003
- 2003-12-12 CN CNB2003101159233A patent/CN1300324C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642466B (en) * | 2009-05-12 | 2013-01-30 | 王振宇 | Preparation method of composition containing pilose antler polysaccharides and having function of reducing blood lipids |
| CN102987501A (en) * | 2012-10-17 | 2013-03-27 | 徐州鸿宇农业科技有限公司 | Method for preparing nutrient (liquid) beverages and granules of healthcare food (therapy) products by using extractives of cordyceps militaris and cocoons |
| CN102987501B (en) * | 2012-10-17 | 2013-11-06 | 徐州鸿宇农业科技有限公司 | Method for preparing nutrient (liquid) beverages and granules of healthcare food (therapy) products by using extractives of cordyceps militaris and cocoons |
| CN106432530A (en) * | 2016-11-24 | 2017-02-22 | 福建省农业科学院土壤肥料研究所 | Extracting technology of dictyophora indusiata volva colloid polysaccharide |
| CN106432530B (en) * | 2016-11-24 | 2019-04-12 | 福建省农业科学院土壤肥料研究所 | A kind of extraction process of dictyophora phalloidea volva colloid polysaccharide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1300324C (en) | 2007-02-14 |
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