CN1541691A - Acute icterohepatitis treating Chinese traditional medicine and its preparation - Google Patents
Acute icterohepatitis treating Chinese traditional medicine and its preparation Download PDFInfo
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Abstract
The Chinese medicine for treating acute icterohepatitis is prepared with snowbell-leaf tickclover, giant knotwood, selfheal, tuckahoe and date. The synergistic effect of the ingredients makes the Chinese medicine possess functions of clearing away heat and toxic matter, promoting diuresis and eliminating icterus and thus obvious curative effect on icterohepatitis. In addition, the Chinese medicine has wide material source and low cost.
Description
Technical field:
The present invention relates to a kind of Chinese medicine for the treatment of acute icterohepatitis and preparation method thereof.
Background technology:
Acute icterohepatitis is higher at China's sickness rate, has only the Chinese medicine of some treatment icterohepatitises at present in the known technology, and the employing Rhizoma Polygoni Cuspidati of stepping on as " Hubei Journal of Traditional Chinese Medicine " nineteen eighty-three the 4th periodical 90 restrains, be decocted in water for oral dose, every day, potion was obeyed several weeks even several months, continuously until transference cure.The deficiency of this Therapeutic Method is that the course of treatment is long, and cure rate neither be very high, on the Chinese patent communique we can see application number be the patent application of " 01118744.1 " disclosed also be a kind of Chinese medicine for the treatment of icterohepatitis, its prescription is Radix Codonopsis 14-16, Cornu Cervi Pantotrichum 4-6, Radix Glycyrrhizae 8-12, Herba Artemisiae Scopariae 52-54, Radix Et Rhizoma Rhei 11-13, then the prescription medicine cost is higher for this, especially the expensive Chinese medicine of Cornu Cervi Pantotrichum generic name.Chinese medicine for the special for treating acute icterohepatitis has had not yet to see report.
Summary of the invention:
The object of the present invention is to provide a kind of short treating period, therapeutic effect significantly, medicine source Chinese medicine that is exclusively used in the treatment acute icterohepatitis extensive, with low cost and preparation method thereof.
Chinese medicine of the present invention comprises following component by weight percentage: Herba Desmodii Styracifolii 10%-80%, Rhizoma Polygoni Cuspidati 5%-60%, Spica Prunellae 5%-30%, Poria 0-20%, Fructus Jujubae 0-10%.
Chinese medicine of the present invention optimum ratio by weight percentage is: Herba Desmodii Styracifolii 57.14%, Rhizoma Polygoni Cuspidati 14.28%, Spica Prunellae 14.28%, Poria 9.53%, Fructus Jujubae 4.76%.
The method for preparing this Chinese medicine: get Rhizoma Polygoni Cuspidati, the Spica Prunellae of above-mentioned prescription amount, with alcohol heating reflux extraction to a certain degree; Filtrate is condensed into thick paste; Medicinal residues and Herba Desmodii Styracifolii, Poria, Fructus Jujubae merge, extracting in water, and gained water is carried filtrate and is condensed into thick paste, and gained water carries thick paste and above-mentioned alcohol extraction gained thick paste merges, and adds adjuvant and granulate.
The best approach in above-mentioned preparation method is: get Rhizoma Polygoni Cuspidati, the Spica Prunellae of described prescription amount, coarse crushing respectively adds 8 times of amount 85% ethanol, and heating and refluxing extraction 1.5 hours is filtered.Medicinal residues add 7 times of amount 85% ethanol again, and heating and refluxing extraction 1 hour is filtered.Merging filtrate, ethanol is reclaimed in decompression (temperature 50-60 ℃, vacuum 0.06-0.08Mpa), and is concentrated into the thick paste that relative density is 1.27-1.30 (60 ℃).Medicinal residues merge with Herba Desmodii Styracifolii, Poria, Fructus Jujubae again, add the water of 14 times of amounts, soaking at room temperature 1 hour, and ebuillition of heated extracted 1.5 hours, filtered.Medicinal residues add the water of 9 times of amounts again, and ebuillition of heated extracted 1 hour, filter.Merge gained water and carry filtrate, decompression (temperature 65-70 ℃, vacuum 0.06-0.08Mpa) is concentrated into the thick paste that relative density is 1.27-1.30 (60 ℃), and gained water carries thick paste and above-mentioned alcohol extraction gained thick paste merges.Add dextrin, mixing, the shop layer, vacuum drying gets pure dried cream.Add sucrose, dextrin again, pulverize, sieve, mixing.With 90% ethanol system soft material, cross 14 mesh sieves and granulate, about 60 ℃ dry 1.0-1.5 hour earlier, behind the granulate again elevated temperature to 80 ℃ be dried to water content less than 5%, promptly.
The traditional Chinese medical science is thought acute icterohepatitis morbidity, is the damp invasion, and plain body stomach-fire is prosperous partially, conversion of dampness into heat and retention of damp-heat in the interior.The liver failing to maintain the normal flow of QI, the bile flowing outside the biliary passages, on annotate in order, the skin that exosmoses oozes down bladder, then body icteric sclera, yellowish urine.Burnt taste in the retention of damp-heat in the interior, stomach-QI being unable to descend normally, adverse rising of stomach-QI, spleen lose fortuneization, can not support muscle, so nauseating, poor appetite, detest greasy, upper abdomen distension, spiritlessness and weakness.Tongue tip side of red, yellow and greasy fur, slippery and rapid pulse are the performance of retention of damp-heat in the interior.So Chinese medicine of the present invention is selected the prescription with the effect of heat-clearing and toxic substances removing, dampness removing jaundice eliminating, especially selecting Herba Desmodii Styracifolii is monarch, because Herba Desmodii Styracifolii has heat-clearing and toxic substances removing, relieving stranguria by diuresis, the effect of dehumidifying jaundice eliminating, the prescription of do treatment cholelithiasis commonly used in known technology, and the present invention is monarch with it, utilize the property of medicine of its dampness removing diuretic to make damp and hot from the urine discharge, thereby to the treatment icterohepatitis especially acute icterohepatitis played important effect, be aided with Rhizoma Polygoni Cuspidati is minister in addition, and Rhizoma Polygoni Cuspidati has heat-clearing and toxic substances removing, dampness removing jaundice eliminating, the blood stasis expelling of invigorating blood circulation, pathogenic fire purging relieving constipation.Its pathogenic fire purging defecating feces excretion makes damp and hot from defecating.Spica Prunellae can the kind clearing away liver-fire of clearing away heat and cooling blood.Its heat-clearing and toxic substances removing, soothing the liver row stagnate to strengthen the effect of Herba Lysimachiae, Rhizoma Polygoni Cuspidati.Assistant is with Poria spleen invigorating invigorating middle warmer, promoting diuresis to eliminate damp pathogen, and Fructus Jujubae strengthens the spleen and stomach, and the strong then wet facilitation of water of transporting of taste for making, relaxes all medicine property of medicine with it, prevents the imapirment of the spleen and stomach of bitter cold.All medicine mutually 5, clear profit is fallen and is let out, and draws damp and hotly just to go from two, and heresy has outlet, and is damp and hot from removing.Play the effect of heat-clearing and toxic substances removing, dampness removing jaundice eliminating altogether.Make treatment by Chinese herbs acute icterohepatitis of the present invention have significant effect.
The inventor has also proved the significance of Chinese medicine curative effect of the present invention by zoopery and clinical case, below be animal experiment method and result and with treatment by Chinese herbs acute icterohepatitis patient's of the present invention some cases:
Test material:
1, experimental animal:
Mice: Kunming kind, 18-22g, ♀ ♂ dual-purpose, the Jiangxi College of Traditional Chinese Medicine Experimental Animal Center provides.
Rat: SD is male, and body weight is about 200-300, and animal housing of Jiangxi Medical College provides.
2, medicine and reagent:
(1) extractum of the present invention: specification: 4.24g crude drug/g extractum.
Mice with dosage is:
Low dosage: 6.8g crude drug/Kg, promptly get 6.8/4.24/1.16
*4=5.53ml extractum adds normal saline to 100ml;
Middle dosage: 13.6g crude drug/Kg, promptly get 13.6/4.24/1.16
*4=11.06ml extractum adds normal saline to 100ml;
High dose: 27.2g crude drug/Kg, promptly get 27.2/4.24/1.16
*4=22.12ml extractum adds normal saline to 100ml.
Rat with dosage is:
Low dosage: 4.7g crude drug/Kg, promptly get 4.9/4.24/1.16
*20=19ml extractum stock solution adds normal saline to 200ml;
Middle dosage: 9.4g crude drug/Kg, promptly get 9.4/4.24/1.16
*20=38ml extractum adds normal saline to 200ml;
High dose: 18.8g crude drug/Kg, promptly get 18.8/4.24/1.16
*20=76ml extractum adds normal saline to 200ml.
(2) the grand electuary of sharp liver: Luzhou Baoguang Pharmaceutical Industrey Co., Ltd., every bag of 10g of specification, three times on the one, a 10g converts to such an extent that mice dosage is 3.88g crude drug/Kg by body surface area method and people's dosage; Administration volume: 25ml/Kg, compound method: accurately take by weighing the grand granule of the sharp liver of 15.52g and add normal saline to l00ml; Convert to such an extent that rat dosage is 2.7g crude drug/Kg by body surface area method and people's dosage; Administration volume: l0ml/Kg, compound method: accurately take by weighing the grand granule of the sharp liver of 54g and add normal saline to 200ml.
(3) carbon tetrachloride: CR, Shanghai reagent purchasing and supply station faces with preceding and is made into 0.1% solution with vegetable oil.
(4) D-galactosamine (GalN): Sigma company product, face with the preceding D-galactosamine 1000mg that accurately takes by weighing, add physiological saline solution to 12.5ml, with 1MNaOH pH value is transferred to 7.0.
(5) Cyclophosphamide for injection: 0.2g * 5 bottle, adult 0.2g, once a day, 1 bottle of cyclophosphamide (200ml) is got by the permanent auspicious medical limited company in Jiangxi, adds the injection water to 50ml, and is now with the current.
(6) Dou Shi reagent: sodium bicarbonate 1.0g, potassium cyanide 0.05g, high-potassium ferricyanide 0.2g, distilled water 1000ml surveys hemoglobin and uses.
(7) complement: be guinea pig serum: get healthy guinea pig blood, separation of serum, with being for experiment after the normal saline dilution in 1: 10, former serum is preserved standby in-20 ℃ of refrigerators.
(8) α-ANIT (ANIT): the Sigma product is made into 80mg/ml with vegetable oil.
(9) SRBC (sheep red blood cell (SRBC)): the male doctor of Nanchang City's remittance inspection center.
(10) SGPT test kit: Shanghai Rongsheng Bioisystech Co., Ltd.
(11) total bilirubin testing cassete: Zhejiang Province Yilikang Bioisystech Co., Ltd; The SGPT testing cassete is for giving birth to company's product in Beijing.
(12) 20% urethanes: chemical plant, sun middle school, business section, Shanghai product, take by weighing urethane 20 grams, adding distil water is to 100ml.
3, administration capacity: mouse stomach is the 25ml/kg body weight, and rat oral gavage is the 10ml/kg body weight.
4, statistical disposition: experimental data is carried out statistical disposition with the SPSS system, checks with t according to the distribution situation of data, or t ' check, to draw corresponding conclusion.
Test method and result:
One, Chinese medicine of the present invention is to the influence of carbon tetrachloride induced mice acute liver damage
1, animal grouping: be divided into 6 groups at random, every group 10, be Chinese medicine low dose group of the present invention (6.8g crude drug/Kg), middle dosage group (13.6g crude drug/Kg), high dose group (27.2g crude drug/Kg), the grand group of sharp liver (3.88g/kg), carbon tetrachloride model group and normal saline group.
2, each treated animal carries out gastric infusion respectively, be that concentration medicine, Chinese medicine high dose group of the present invention are irritated stomach high concentration medicine, the grand grand suspension of filling stomach profit liver of organizing of sharp liver in normal saline group, model group filling stomach normal saline, Chinese medicine low dose group of the present invention filling stomach low concentration medicine, the Chinese medicine of the present invention filling stomach, the administration volume is 25ml/Kg, once a day, successive administration 6 days, administration in morning every day.All the other respectively organized mouse peritoneal injection 0.1%CCI except that the normal saline group in 1 hour behind the last filling stomach
4, capacity is: 10ml/kg; Each treated animal is plucked eyeball and is got blood after 24 hours, place, distinguish serum, carry out SGPT (glutamate pyruvate transaminase) and put to death animal, take out liver and carry out the pathomorphology inspection, the results are shown in Table 1, table 2 (the model group animal has 2 haemolysis, and normal saline and Chinese medicine high dose group of the present invention respectively have 1 to cause haemolysis accidentally because of getting blood, so number of samples has only 8 or 9);
Table 1 Chinese medicine of the present invention is to the influence of carbon tetrachloride induced mice acute liver damage
Group number of animals dosage (/Kg) SGPT (U)
Normal saline 9 25ml 33.89 ± 14.42
CCl
4Model group 8 10ml 298.75 ± 222.29
△ △ △
Chinese drug-treated group of the present invention is hanged down 10 6.8g crude drugs 192.10 ± 172.67
▲
In 10 13.6g crude drugs 47.70 ± 17.67
▲ ▲
High 9 37.2g crude drugs 35.88 ± 20.32
▲ ▲
The grand group of sharp liver 10 3.88g 57.80 ± 30.35
▲ ▲
Adopt the t check, compare with the normal saline group,
△ △ △P＜0.001; Compare with model group,
▲P＜0.05,
▲ ▲P＜0.01.
As can be seen from Table 1, CCl
4Model group animal serum GPT value obviously raises, illustrate that modeling is successful, except that Chinese medicine low dose group of the present invention, the grand group of the Chinese medicine of the present invention of middle and high dosage group and sharp liver all can reduce serum GPT value, and the acute liver damage effect due to the antagonism carbon tetrachloride is described.
Table 2 Chinese medicine of the present invention is to the pathological examination result of carbon tetrachloride induced mice acute liver damage
It is a plurality of that visual field of each visual field point is put in several visuals field
The group number of animals does not have focus
The necrosis of the downright bad point-like lamellar of the downright bad shape lamellar of shape lamellar
Normal saline 10 10 000
CCl
4Model group 10 0
△0
△5
△10
△
Chinese drug-treated group of the present invention low 10 03
▲64
▲ ▲ ▲
In 10 02
▲75
▲ ▲ ▲
High by 10 2
▲5
▲ ▲ ▲41
▲ ▲ ▲
The grand group 10 0076 of sharp liver
▲
Adopt X 2 test, compare with the normal saline group,
△P＜0.001; Compare with model group,
▲P＜0.05,
▲ ▲P＜0.01,
▲ ▲ ▲P＜0.001.
Murine liver tissue 10% formalin fixed, the routine paraffin wax section, HE dyeing, 10 * 10 times of mirrors are observed down, find: normal saline treated animal liver organization structure is normal, the model group liver organization is popularity, point-like or lamellar focus, be distributed in around the central vein more, focus shows as hepatocellular degeneration, swelling, necrosis, structural fuzzy, and with inflammatory cell infiltration, all appearance point lamellar necrosis in 10 animals, each visual field bad the dead of point-like lamellar has 5, and a plurality of lamellars or spotty necrosis can be seen in visual field of 10 animal pathological sections.Sharp liver grand group focus and model group are in a ratio of slight change, and Chinese medicine low dose group part mouse liver tissue lesions of the present invention dwindles, and pathological changes alleviates; The dosage group is similar to low dose group in the Chinese medicine of the present invention; Chinese medicine high dose group mice of the present invention is compared with model group, and focus obviously reduces, and pathological changes alleviates, and 2 mices is arranged near normal group.
Two, Chinese medicine of the present invention is to the influence of D-galactosamine hydrochlorate acute liver damage model mice
1, animal grouping: be divided into 6 groups at random, every group 10, it is Chinese medicine low dose group of the present invention (6.8g crude drug/Kg), middle dosage group (13.6g crude drug/Kg), high dose group (27.2g crude drug/Kg), the sharp grand group of liver (3.88g/kg), D-galactosamine model group (800mg/Kg) and normal saline group.
2, observation index: each treated animal carries out gastric infusion respectively, be that concentration medicine during the dosage group is irritated in normal saline group, model group filling stomach normal saline, Chinese medicine low dose group of the present invention filling stomach low concentration medicine, the Chinese medicine of the present invention, Chinese medicine high dose group of the present invention are irritated stomach high concentration medicine, the grand grand suspension of the sharp liver of filling stomach of organizing of sharp liver, the administration volume is 25ml/Kg, once a day, successive administration 6 days, administration in morning every day.1 hour all the other mouse peritoneals injection 80mg/mlD-galactosamine at different levels except that normal saline behind the last filling stomach, capacity is: 10ml/mg; Each treated animal is plucked eyeball and is got blood after 24 hours, places, and distinguishes serum, carries out SGPT (glutamate pyruvate transaminase) and puts to death animal, takes out liver and carries out the pathomorphology inspection, the results are shown in Table 3, table 4:
Table 3 Chinese medicine of the present invention is to the influence of D-galactosamine induced mice acute liver damage
Group number of animals dosage (/Kg) SGPT (U)
Normal saline 10 25ml 12.90 ± 7.31
CCl
4Model group 8 10ml 174.13 ± 79.63
△
Chinese drug-treated group of the present invention is hanged down 9 6.8g crude drugs 248.88 ± 98.80
In 10 13.6g crude drugs 64.50 ± 29.44
▲ ▲
High 10 27.2g crude drugs 68.60 ± 42.72
▲
The grand group of sharp liver 10 3.88g 107.00 ± 42.89
▲
Adopt t ' check, compare with the normal saline group,
△P＜0.001; Compare with model group,
▲P＜0.05,
▲ ▲P＜0.01,
▲ ▲ ▲P＜0.001.
As can be seen from Table 3, D-galactosamine can cause the chmice acute hepatic injury, D galactosamine model group mice serum GPT value obviously raises, illustrate that modeling is successful, except that Chinese medicine low dose group of the present invention, the grand group of the Chinese medicine of the present invention of middle and high dosage group and sharp liver all can reduce serum GPT value, and explanation is the acute liver damage effect due to the antagonism D-galactose obviously.
Table 4 Chinese medicine of the present invention is to the pathological examination result of D-galactosamine induced mice acute liver damage
The group number of animals is soaked into the necrosis of degeneration edema
Normal saline 10 000
CCl
4Model group 10 7
△ △ △10
△ △ △2
△
Chinese drug-treated group of the present invention low 10 581
In 10 3
▲80
▲
High by 10 2
▲7
▲0
▲
The grand group 10 4 of sharp liver
▲10 0
▲
Adopt X 2 test, compare with the normal saline group,
△P＜0.05,
△ △ △P＜0.001; Compare with model group,
▲P＜0.05.
Hepatic tissue pathology section microscopy is found: normal group liver organization structure is normal, and the model group murine liver tissue shows as the degeneration of dispersivity hepatic tissue, edema necrosis, have with inflammatory cell infiltration, severe patient shows as necrosis.The grand group of sharp liver has lighter improvement not obvious, and Chinese medicine low dose group medicine of the present invention improves not obvious, and middle dosage group has slight improvement effect, and the high dose group pathological changes has clear improvement.
Three, Chinese medicine of the present invention is to the influence of mice serum hemolysin
1, animal grouping: be divided into 6 groups at random, every group 12, be Chinese medicine low dose group of the present invention (6.8g crude drug/Kg), middle dosage group (13.6g crude drug/Kg), high dose group (27.2g crude drug/Kg), the grand group of sharp liver (3.88g/kg), cyclophosphamide model group (40mg/Kg) and normal saline group.
2, observation index: each treated animal carries out gastric infusion respectively: promptly concentration medicine Chinese medicine high dose of the present invention is irritated the stomach high concentration medicine in the dosage filling stomach in normal saline group, model group filling stomach normal saline Chinese medicine low dosage filling of the present invention stomach low concentration medicine, the Chinese medicine of the present invention, sharp liver is swelled and organizes the grand suspension of filling stomach profit liver, the administration volume is 25ml/Kg, once a day, successive administration 7 days, administration in morning every day.Except that the normal saline group, other each treated animal subcutaneous injection 4mg/ml cyclophosphamide 10ml/kg, for three days on end, got in the 4th day and to dilute good SRBC0.2ml ip in mice, last is irritated behind the stomach 1 hour mice and is plucked eyeball and get blood, with drop of blood to centrifuge tube, placed 1 hour under the room temperature, solidification blood and tube wall are peeled off, serum is fully oozed out with thin Glass rod, after 1 hour with 2000 rev/mins centrifugal 10 minutes, get upper serum; Isolated serum is diluted 250 times with normal saline, and (20ul to 5ml) in 56 ℃ of water-baths, 30 minutes deactivation complements are got this blood serum sample 1ml and are added in the reaction tube, add 0.5mlSRBC (2 * 10 again
9/ ml), put in the ice bath cooling after, in every pipe, add the 1ml complement, 37 ℃ of insulations 10 minutes, after the insulation reaction tube is moved in the ice bath, with cessation reaction immediately then.With reaction tube with 2000 rev/mins centrifugal 10 minutes, get supernatant and carry out hemoglobin colour comparison and measure.The sample blank control tube, outside 1ml physiologic saline for substitute blood serum sample, all same reaction tube of SRBC and complement addition and operating procedure.Get above-mentioned centrifugal gained supernatant 1ml and 3ml Dou Shi reagent and place test tube fully to shake up, put 10 minutes after because whole hemoglobin that haemolysis produces become cyanohemoglobin, present stable redness, in the 540nm colorimetric.Trap value when determination test is used the SRBC HD50; The trap value of hemoglobin when each test all should be measured used SRBC HD50.Promptly get the SRBC through washing and dilution of 0.25ml test usefulness, be diluted to 4ml, shake up, placed 10 minutes with Dou Shi reagent, in the 540nm colorimetric, record trap value.Trap value when this value is used SRBC HD50 in the test.Trap value * extension rate during the half hemolysis value HC50=sample light absorption value of sample/SRBC HD50, result of the test sees Table 5:
Table 5 Chinese medicine of the present invention is to the influence of mice serum hemolysin
Group number of animals dosage (/Kg) hemolysin
Normal saline 12 25ml 132.53 ± 34.56
Cyclophosphamide group 12 40mg 5.17 ± 0.72
△
Chinese drug-treated group of the present invention is hanged down 10 6.8g crude drugs 37.29 ± 5.10
▲
In 12 13.6g crude drugs 29.61 ± 3.79
▲
High 12 27.2g crude drugs 29.61 ± 3.79
▲
The grand group of sharp liver 12 3.88g 27.63 ± 4.11
▲Adopt t ' check, compare with the normal saline group,
△P＜0.001; Compare with model group,
▲P＜0.001.
As can be seen from Table 5, this immunosuppressant of cyclophosphamide can cause the mice serum hemolysin level to descend, the modeling success is described, basic, normal, high dosage group of Chinese medicine of the present invention and sharp liver are grand all can very obviously improve the mice serum hemolysin level, and the mouse humoral immune function is had obvious facilitation.(annotate: low dose group is owing to have two haemolysis when getting blood, so number of samples is 10)
Four, Chinese medicine of the present invention causes the influence of cholestasis type jaundice rat to α-ANIT (ANIT)
1, animal grouping: be divided into 6 groups at random, every group 10, be Chinese medicine low dose group of the present invention (4.7g crude drug/Kg), middle dosage group (9.4g crude drug/Kg), high dose group (18.8g crude drug/Kg), the grand group of sharp liver (2.7g/kg), ANIT model group and normal saline group.
2, observation index: each treated animal carries out gastric infusion respectively: promptly the dosage group is irritated concentration medicine in the stomach in normal saline group, model group filling stomach normal saline, Chinese medicine low dose group of the present invention filling stomach low concentration medicine, the Chinese medicine of the present invention, Chinese medicine high dose group of the present invention is irritated stomach high concentration medicine, the grand grand suspension of the sharp liver of filling stomach of organizing of sharp liver, the administration volume is 10ml/kg, once a day, successive administration 6 days, the administration of noon every day.Last irritate stomach after 1 hour except that the normal saline group.Other each treated animals are pressed 80mg/kg and are irritated stomach ANIT once; Water is can't help in each treated animal fasting; After rat is anaesthetized with urethane 60mg/kgip after 24 hours, face upward the position and be fixed on the fixing head, cut about 2cm along the abdomen median line, open the abdominal cavity, find stomachus pyloricus, the upset duodenum, in the descendant duodenum mesentery, find white flexible bile duct, under it, wear 2 rhizoid lines, the ligation pars papillaris, intraduodenal administration 10ml/mg, make V-notch to the liver direction after 30 minutes, insert plastic tube, promptly visible faint yellow bile flows out, ligation fixed plastics pipe is collected bile with small beaker.Used after operation hemostasis clamp closes stomach wall, covers with saline gauze, collects bile once every 30min after the administration, and totally 8 times, record bile flow, bile flow after the calculating administration.After having collected bile, the animal femoral vein is got blood, carries out SGPT, total bilirubin determination, the results are shown in Table 6-1, table 6-2, table 7:
Table 6-1 Chinese medicine of the present invention causes the influence of cholestasis type jaundice rat bile amount to α-ANIT (ANIT)
Animal
The bile total flow
Group
Dosage (/kg)
30min 60min 90min
Number
(ml)
Normal control group 9 25ml 3.81 ± 0.33 0.61 ± 0.12 0.49 ± 0.10 0.47 ± 0.09
ANIT organizes 9 100mg 2.11 ± 0.66
△0.40 ± 0.14
△0.31 ± 0.15
△0.30 ± 0.13
△
Chinese medicine 9 4.7g crude drugs 3.54 ± 0.20 of the present invention
▲0.56 ± 0.06
▲0.52 ± 0.05
▲0.50 ± 0.03
▲
9 9.4g crude drugs 3.79 ± 0.40
▲0.63 ± 0.06
▲0.57 ± 0.06
▲0.56 ± 0.08
▲
8 18.8g crude drugs 3.66 ± 0.37
▲0.59 ± 0.15
▲0.54 ± 0.09
▲0.51 ± 0.07
▲
The grand group of sharp liver 8 2.7g 2.45 ± 0.69 0.48 ± 0.10 0.39 ± 0.09 0.31 ± 0.10
Adopt the t check, compare with the normal saline group,
△P＜0.01; Compare with model group,
▲P＜0.01.
Table 6-2 Chinese medicine of the present invention causes the influence of cholestasis type jaundice rat bile amount to α-ANIT (ANIT)
Group 120min 150min 180min 210min 240min
Normal control group 0.48 ± 0.07 0.50 ± 0.05 0.43 ± 0.10 0.44 ± 0.10 0.39 ± 0.09
ANIT group 0.24 ± 0.12
△0.23 ± 0.08
△0.20 ± 0.11
△0.18 ± 0.06
△0.14 ± 0.05
△
Chinese medicine of the present invention low 0.47 ± 0.03
▲0.43 ± 0.03
▲0.39 ± 0.04
▲0.35 ± 0.04
▲0.32 ± 0.04
▲
In 0.48 ± 0.07
▲0.44 ± 0.03
▲0.40 ± 0.06
▲0.38 ± 0.06
▲0.33 ± 0.06
▲
High by 0.47 ± 0.05
▲0.43 ± 0.06
▲0.43 ± 0.04
▲0.37 ± 0.06
▲0.32 ± 0.04
▲
The grand group 0.31 ± 0.11 0.27 ± 0.10 0.27 ± 0.11 0.24 ± 0.08 0.19 ± 0.06 of sharp liver
Table 7 Chinese medicine of the present invention causes the influence of cholestasis type jaundice rat GPT and Tbil to α-ANIT (ANIT)
Group number of animals dosage (/Kg) SGPT (U) Tbil (mg/dl)
Normal control group 10 25ml 14.70 ± 8.50 0.938 ± 0.110
ANIT organizes 9 100mg 168.44 ± 36.37
△2.964 ± 0.431
△
Chinese drug-treated group of the present invention is hanged down 8 4.9g crude drugs 131.78 ± 23.95
▲2.473 ± 0.433
▲In
9 9.4g crude drugs 64.50 ± 28.26
▲2.094 ± 0.318
▲
High 8 18.8g crude drugs 38.00 ± 26.14
▲1.855 ± 0.319
▲
The grand group of sharp liver 8 2.7g 84.00 ± 43.97
▲2.149 ± 0.567
▲
As can be seen from the above table, ANIT group rat bile flow obviously reduces, and serum GPT, Tbil obviously raise, the modeling success is described, the basic, normal, high dosage group of Chinese medicine of the present invention medicine all can very obviously increase the rat bile flow, reduce serum GPT, Tbil, illustrate that this medicine has the effect of good promoting the function of the gallbladder to alleviate jaundice.(annotate: normal control group, ANIT organize, Chinese medicine of the present invention is basic, normal, high and the grand group of function of gallbladder promoting has 1,1,1,1,1,2 animal to insert the bile duct failure respectively, so do not obtain gallbladder juice; Remove the normal control group, other each groups are owing to haemolysis occurs when getting blood, so there are not 10 specimen.)
Conclusion:
(1) Chinese medicine of the present invention has the certain protection effect to the chmice acute hepatic injury due to the carbon tetrachloride, and can reduce serum GPT value.
(2) Chinese medicine of the present invention has the certain protection effect to the chmice acute hepatic injury due to the D-galactosamine, and can reduce serum GPT value.
(3) the basic, normal, high dosage of Chinese medicine of the present invention can improve the mouse humoral immune function, and mice serum hemolysin value can obviously raise.
(4) the basic, normal, high dose drug of Chinese medicine of the present invention has the effect of tangible promoting the function of the gallbladder to alleviate jaundice, can obviously increase α-ANIT (ANIT) and cause cholestasis type jaundice rat bile flow, obvious decline effect be arranged for serum GPT, the total bilirubin of its rising.
Five, use treatment by Chinese herbs acute icterohepatitis patient's of the present invention some cases:
Case one: Cao, man, 22 years old, driver, admission number 15453.On June 26th, 1998 was in hospital because of poor appetite, weak, yellowish urine in 5 days, and the companion feels sick, body icteric sclera, epigastric discomfort, dry stool, red tip of the tongue, yellow and thin fur, slippery and rapid pulse.Have a medical check-up skin and sclera degree of depth xanthochromia.The preceding outer court of being admitted to hospital looks into liver function ALT1230IV/L, AST1029IV/L, TBiL169umol/L, and hepatitis B six HBsAg, HBeAg, the HBcAb positive are diagnosed as the acute icteric hepatitis B.With Chinese medicine agreement Fang Weizhu of the present invention, day potion, decocting divides the secondary clothes, cooperates auxiliary treatment such as felviteen, motilium check on the 18th liver function ALT, AST normal.Six HbeAg of TBil70 umol/L hepatitis B are cloudy to be changeed, remission, and jaundice alleviates automatically and leaves hospital.10 doses of the former sides of clothes are continued in the back of leaving hospital, and check liver function ALT, AST, TBiL are all normal, and jaundice disappears.
Case two: Tang, man, 26 years old, cadre, admission number 15604.Because of feel sick, vomiting, poor appetite, weak, heating be in hospital two weeks on July 16th, 1998, before being admitted to hospital once by " acute hepatitis " outside gate to examine with potenlin be main one week of liver protecting therapy, symptom does not have alleviation, goes out to appear icteric sclera and comes my institute, 37.5 ℃ of skins of the temperature of having a medical check-up and the slight xanthochromia of sclera.Chemical examination liver function ALT706IV/L, TBiL76umol/L, the anti-HAV-IgM positive.Be diagnosed as the acute icteric hepatitis A.With Chinese medicine of the present invention agreement Fang Weizhu, day potion, decocting divides the secondary clothes, cooperates auxiliary treatment and vein makeup energy mixture such as compound vitamin B, treat 11 days check liver ALT, AST, AbiL are all normal, skin and yellow sclera disappearance, remission is left hospital.
Case three: rely certain, man, 9 years old, admission number 15612.Because of poor appetite, weak, yellowish urine 10 days were admitted to hospital on July 17th, 1998.Premorbid has unhygienic diet shell seafood history.37.4 ℃ of companion, the temperature of having a medical check-up, pain is kowtowed in soft, the tenderness of 1cm matter, hepatic region under severe xanthochromia in skin and the sclera, the right rib of liver.Be admitted to hospital and look into liver function ALT1601IV/L, TBiL123umol/L, the anti-HAV-IgM positive.Be diagnosed as the acute icteric hepatitis A.With half agent of Chinese medicine of the present invention agreement side, every day, decocting divided the secondary clothes, cooperated the treatment of vein makeup energy.Check ALT59IV/L, AST53IV/L, TBiL59umol/L, remission after 17 days, the little Huang of sclera is left hospital automatically, follow-up the taking traditional Chinese medicine former 1 week of side of leaving hospital, and check liver function ALT, AST, TBiL are all normal, and yellow sclera disappears.
Case four: Xu Yan, the woman, 24 years old, unemployed.Admission number 16659.On July 22nd, 1999 because of poor appetite, weak, be admitted to hospital in 1 week of yellowish urine.Occur together heat, dry stool, upper abdomen distension are had a medical check-up T37.6 ℃.Skin and sclera moderate xanthochromia, red tongue, yellow fur after a while, slippery and rapid pulse.Look into liver function ALT850IV/L, AST671IV/L, TBiL54umol/L, six HBsAg of hepatitis B, the HBcAb positive.Be diagnosed as the acute icteric hepatitis B.With Chinese medicine agreement Fang Weizhu of the present invention, day potion, decocting, divide the secondary clothes, this cooperates treatments such as oral compound vitamin B, vein input liver peace, makeup energy 22 days, and check liver function ALT, AST are normal, TBiL25.3umol/L, remission, skin and yellow sclera disappear and leave hospital.
Case five, Zhang Xiaoguo, man, 36 years old, cadre, admission number 16832.Because of poor appetite, weak, yellowish urine 10 days were in hospital on August 13rd, 1999.Be admitted to hospital preceding 3 months once because of the blood transfusion of brain trauma surgery.Companion when being admitted to hospital, big constipation, body of the tongue are light red, yellow and thin fur, stringy pulse.The skin of having a medical check-up is gone into the light moderate xanthochromia of sclera, and is surplus no abnormal.Chemical examination liver function ALT2297IV/L, TBiL82umol/L, hepatitis B six HBsAg, HBeAg, the HBcAb positive, the weak positive of HBV-DNA.Be diagnosed as the acute icteric hepatitis B.With Chinese medicine agreement Fang Weizhu of the present invention, day potion, decocting divides the secondary clothes, cooperates vein makeup energy mixture, treatments such as liver peace were checked liver function ALT on 18th, AST, TBiL are normal, the cloudy commentaries on classics of six HbeAg of hepatitis B, HBV-DNA feminine gender, remission, skin and yellow sclera, disappear, 1 week of after treatment leaves hospital.
Above-mentioned zoopery and part patient medical record can find out that all Chinese medicine of the present invention has extraordinary effect to the treatment acute icterohepatitis, and its medicine source is extensive, with low cost simultaneously, is a kind of special-purpose Chinese medicine of good treatment acute icterohepatitis.
The specific embodiment:
Embodiment one:
Herba Desmodii Styracifolii 3333.3g, Rhizoma Polygoni Cuspidati 833.3g, Spica Prunellae 833.3g, Poria 555.6g, Fructus Jujubae 277.8g.Get Rhizoma Polygoni Cuspidati, the Spica Prunellae of above-mentioned recipe quantity, coarse crushing respectively adds 8 times of amount 85% ethanol, and heating and refluxing extraction 1.5 hours is filtered.Medicinal residues add 7 times of amount 85% ethanol again, and heating and refluxing extraction 1 hour is filtered.Merging filtrate, ethanol is reclaimed in decompression (temperature 50-60 ℃, vacuum 0.06-0.08Mpa), and is concentrated into the thick paste that relative density is 1.27-1.30 (60 ℃).Medicinal residues merge with Herba Desmodii Styracifolii, Poria, Fructus Jujubae again, add the water of 14 times of amounts, soaking at room temperature 1 hour, and ebuillition of heated extracted 1.5 hours, filtered.Medicinal residues add the water of 9 times of amounts again, and ebuillition of heated extracted 1 hour, filter.Merge gained water and carry filtrate, decompression (temperature 65-70 ℃, vacuum 0.06-0.08Mpa) is concentrated into the thick paste that relative density is 1.27-1.30 (60 ℃), and gained water carries thick paste and above-mentioned alcohol extraction gained thick paste merges, heavily about 1.2kg.Add the 170g dextrin, mixing, the shop layer, vacuum drying (temperature 60-70 ℃, vacuum 0.08-0.09Mpa) gets the about 667g of pure dried cream.Add sucrose 100g, dextrin 63g again, pulverize, sieve, mixing.With 90% ethanol system soft material, cross 14 mesh sieves and granulate, about 60 ℃ dry 1.0-1.5 hour earlier, behind the granulate again elevated temperature to 80 ℃ be dried to water content less than 5%, by every bag of 6g specification packing (about 166 bags), promptly.The three guarantees of order clothes, dosage 18 grams, symphysis medicine 105 grams.
Chinese medicine of the present invention should be made taking convenience, absorbs fast peroral dosage form, as granule, tablet, capsule.According to pharmaceutical properties and dose, the granule that select disintegrate, dissolve soon, is easy to absorb is better.
The Chinese prescription amount according to the present invention below also provides 3 embodiments to optimum ratio of the present invention (weight percent meter):
Claims (4)
1, a kind of Chinese medicine that is used for the treatment of acute icterohepatitis comprises following component by weight percentage: Herba Desmodii Styracifolii 10%-80%, Rhizoma Polygoni Cuspidati 5%-60%, Spica Prunellae 5%-30%, Poria 0-20%, Fructus Jujubae 0-10%.
2, the Chinese medicine of treatment acute icterohepatitis according to claim 1 is characterized in that: this Chinese medicine optimum ratio by weight percentage is: Herba Desmodii Styracifolii 57.14%, Rhizoma Polygoni Cuspidati 14.28%, Spica Prunellae 14.28%, Poria 9.53%, Fructus Jujubae 4.76%.
3, prepare the method for claim 1 or the described Chinese medicine of claim 2, comprising: get Rhizoma Polygoni Cuspidati, the Spica Prunellae of above-mentioned prescription amount, with alcohol heating reflux extraction to a certain degree; Filtrate is condensed into thick paste; Medicinal residues and Herba Desmodii Styracifolii, Poria, Fructus Jujubae merge, extracting in water, and gained water is carried filtrate and is condensed into thick paste, and gained water carries thick paste and above-mentioned alcohol extraction gained thick paste merges, and adds adjuvant and granulate.
4, the preparation method of Chinese medicine of treatment acute icterohepatitis according to claim 3 comprises: get Rhizoma Polygoni Cuspidati, the Spica Prunellae of described prescription amount, coarse crushing respectively adds 8 times of amount 85% ethanol, and heating and refluxing extraction 1.5 hours is filtered.Medicinal residues add 7 times of amount 85% ethanol again, and heating and refluxing extraction 1 hour is filtered; Merging filtrate, ethanol is reclaimed in decompression (temperature 50-60 ℃, vacuum 0.06-0.08Mpa), and is concentrated into the thick paste that relative density is 1.27-1.30 (60 ℃).Medicinal residues merge with Herba Desmodii Styracifolii, Poria, Fructus Jujubae again, add the water of 14 times of amounts, soaking at room temperature 1 hour, and ebuillition of heated extracted 1.5 hours, filtered; Medicinal residues add the water of 9 times of amounts again, and ebuillition of heated extracted 1 hour, filter; Merge gained water and carry filtrate, decompression (temperature 65-70 ℃, vacuum 0.06-0.08Mpa) is concentrated into the thick paste that relative density is 1.27-1.30 (60 ℃), and gained water carries thick paste and above-mentioned alcohol extraction gained thick paste merges; Add dextrin, mixing, the shop layer, vacuum drying gets pure dried cream; Add sucrose, dextrin again, pulverize, sieve, mixing; With 90% ethanol system soft material, cross 14 mesh sieves and granulate, about 60 ℃ dry 1.0-1.5 hour earlier, behind the granulate again elevated temperature to 80 ℃ be dried to water content less than 5%, promptly.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167942B (en) * | 2006-10-27 | 2010-05-26 | 钱浩江 | Medicine for external application |
| CN101352488B (en) * | 2007-07-27 | 2011-03-30 | 北京亚东生物制药有限公司 | Medicament composition for damp-heat type icterohepatitis and preparation method thereof |
| CN102302601A (en) * | 2011-08-23 | 2012-01-04 | 刘世发 | Children jaundice medicine and preparation method thereof |
| CN103005467A (en) * | 2013-01-23 | 2013-04-03 | 中国人民解放军第三军医大学 | Liver-protecting nutritional composition and liver-protecting national food therefrom |
| CN103381250A (en) * | 2013-06-27 | 2013-11-06 | 陶殿华 | Traditional Chinese medicine used for treating icterohepatitis and preparation method thereof |
| CN113197982A (en) * | 2021-05-06 | 2021-08-03 | 彭朝国 | Chinese herbal medicine composition for treating icterohepatitis, preparation method and application |
-
2003
- 2003-11-04 CN CN 200310110490 patent/CN1264540C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167942B (en) * | 2006-10-27 | 2010-05-26 | 钱浩江 | Medicine for external application |
| CN101352488B (en) * | 2007-07-27 | 2011-03-30 | 北京亚东生物制药有限公司 | Medicament composition for damp-heat type icterohepatitis and preparation method thereof |
| CN102302601A (en) * | 2011-08-23 | 2012-01-04 | 刘世发 | Children jaundice medicine and preparation method thereof |
| CN103005467A (en) * | 2013-01-23 | 2013-04-03 | 中国人民解放军第三军医大学 | Liver-protecting nutritional composition and liver-protecting national food therefrom |
| CN103005467B (en) * | 2013-01-23 | 2014-02-19 | 中国人民解放军第三军医大学 | Liver-protecting nutritional composition and liver-protecting nutritional food therefrom |
| CN103381250A (en) * | 2013-06-27 | 2013-11-06 | 陶殿华 | Traditional Chinese medicine used for treating icterohepatitis and preparation method thereof |
| CN113197982A (en) * | 2021-05-06 | 2021-08-03 | 彭朝国 | Chinese herbal medicine composition for treating icterohepatitis, preparation method and application |
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| CN1264540C (en) | 2006-07-19 |
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