CN1530131A - Medicine for treating arthrolithiasis and preparing method thereof - Google Patents
Medicine for treating arthrolithiasis and preparing method thereof Download PDFInfo
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Abstract
An interior-applied Chinese medicine for treating gout and preventing joint deformity, gout complication, uric-acid calculus and tophus is prepared from 6 Chinese-medicinal materials including astractylodes rhizome, phellodendron bark, motherwort, giant knotweed rhizome, etc through proportioning and extracting in alcohol or water. Its advantages are quickly taking its effect, high curative effect and no by-effect.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to a kind of medicine for the treatment of goat and preparation method thereof.
Background technology
Gout is a kind of commonly encountered diseases in European and American countries.Think that in the past it is more rare that gout person suffers from Asian Peoples, but fact proved that sickness rate is on the rise in recent years.Goat is a kind of congenital heredity metabolic deficiency disease, how to be rich in high purine food because of a large amount of absorptions day after tomorrow and to bring out.The human body purine metabolic disturbance easily causes the purine radicals Metabolic disorder, and it is too much to produce uric acid, and renal excretion function generation obstacle causes that blood uric acid increases.Urate more easily in the joint around parenchima place crystallization deposition, cause arthroncus, cause nonspecific inflammation reaction to produce violent pain.Sedimentary uric acid sodium is the pathogenic factor of gout inflammation.
Goat has heritability, and the male sends out well, accounts for whole patients' 95%.Gout (hyperuricemia disease) and hypertension, hyperglycemia, hyperlipidemia also claim " four high diseases ".Gout has the tendency of rejuvenation in recent ten years.The women is more than morbidity (accounting for 5%) after the climacteric menolipsis, this age also be osteoarthritis and chondrocalcinosis send out the age well, easily cause mistaken diagnosis.Infringement is most commonly in the little joint of brothers to gout to osteoarthrosis, for preferably sending out the position, relates to joints such as ankle, knee joint, wrist with foot thumb joint later on.The joint caused inflammation of gout often breaks out, and the joint is red, swollen, hot, tenderness is arranged and have an intense pain, with night more so.Outbreak has General Symptoms such as fervescence often, leukocytosis, and erythrocyte sedimentation rate quickens, serum uric acid salinity rising etc.
Gouty arthritis is divided two kinds of acute gouty arthritis and gouty arthritis,chronics.The redness and swelling of joints of acute gouty arthritis completed stroke, burning pain, stiff, joint stuffiness, have an intense pain limitation of activity and blood uric acid increase and are outstanding behaviours.Gouty arthritis,chronic is meant on the basis that acute arthritis is shown effect repeatedly, causes the destruction of articulation structure and soft tissue, and deformity and limitation of activity in various degree appears in the joint, even the gout calculus occurs.Chronic arthritis is in case after forming, because gout is shown effect repeatedly kidney is badly damaged, joint damage is also more obvious, and difficulty is recovered, the serious threat health.Gouty patient, the metabolic function of sugar and fat can obviously reduce in the body, therefore causes various serious diseases easily, mainly is diabetes, hypertension/hyperlipidemia, obesity, coronary heart disease, myocardial infarction, arteriosclerosis, cerebrovascular disorders etc.
Gout is classified " arthromyodynia " category as on tcm clinical practice.Belong to the traditional Chinese medical science " pyretic arthralgia ", principal character is: the joint is red, swollen, hot, bitterly, joint stuffiness, with heating, fear of cold, aversion to wind, thirsty, restlessness with opprssive feeling and worry etc.With the passing of time outbreak can have subcutaneous nodule to form the ankylosis deformity.Gout is shown effect repeatedly, then can be considered " insensitive impediment " again.Motherland's medical science is thought: primary disease is many because of surfeit delicious food usually, so that retention of damp-heat in the interior, the double damp of having a cold, and the invasion and attack meridians, cold-evil's heat-transformation, damp and hot condensed living expectorant is fled limbs, and the retardance QI and blood meridian is so see that the part redness is scorching hot, sharp ache; If ailment said due to cold or exposure is contained partially, because of wind " change of benefaction number ", so gout does not have the place of deciding, the severe and migratory arthralgia migration; Disease is impairment of the kidney for a long time, and podomere loses supports, so see that deformity is stiff, very then festers.
The treatment goat mainly is to adopt Western medicine such as steroidal anti-inflammatory medicine, glucocorticoid, probenecid, allopurinol, autumn waters--limid eyes alkali at present.Though these Western medicine are alleviating pain rapidly, end the acute attack of gout, alleviate urate deposition in vivo, the formation of prevention gouty arthritis and gouty renal calculus, but this type of medicine has in various degree infringement to gastrointestinal tract, can cause liver, renal dysfunction when serious, takes medicine for a long time, can cause bone marrow depression, hepatic necrosis, oligospermia, neurotoxicity reaction and gastrointestinal reaction, this type of poisonous side effect of medicine is big, is difficult for long clothes.
Patented invention communique on the JIUYUE, last 1999 of Patent Office of the People's Republic of China disclosed a kind of name on the 29th and has been called " compound sanbi medicinal liquor for treating gout ", application number 98111903, the patent application document of publication number CN1229658; Disclosed a kind of name on March 12nd, 1997 and be called " gout miraculous cure wine and preparation technology "; application number 96102851.3; the patent application document of publication number CN1144676A; these two kinds of medicated wine are all made by Chinese medicine; have certain curative effect, but liquor preparation for those can not drink, anemia of pregnant woman, heart disease, hyperpietic, being not suitable for very; and also use the leopard bone in " compound sanbi medicinal liquor for treating gout ", these rules with the protection rare animal are disagreed.Disclosed a kind of name on February 13rd, 2002 and be called " a kind of Chinese medicine preparation ", application number is 01128347, the patent application document of notification number CN1335175; Disclosed a kind of name on October 11st, 2000 and be called that " Sanbiwan bolus, application number are 99112794, the patent application document of notification number CN1269231; Disclosed a kind of name on January 19th, 1994 and be called " a kind of quick-acting gout pill and preparation method thereof ", application number is 93111076, the patent application document of notification number CN1080868, all use the bigger Semen Strychni of toxicity in these in the medicine preparation, though such medicine analgesic effect is stronger, can not take more, obey for a long time but Semen Strychni is for oral administration, excessive poisoning can cause the limbs vibration, faints from fear, breathe because of difficulty, even stupor.The Chinese medicine preparation of some treatment gouts is used some virose insect medicines always or as stronger plant amedica of a class toxicity such as Herba Asari, Radix Aconitis, though certain therapeutical effect is arranged, those weakly patients is not suitable in addition.The present invention is overcoming a kind of unprotect animal kind medicine that proposes on the basis of these inconvenience, no insect medicine, the less pure Chinese medicinal preparation of plant amedica, flavour of a drug that avirulence is stronger just.
Summary of the invention
Purpose of the present invention just provides a kind of more effective, and side effect is little, and is cheap, free of contamination, has the traditional Chinese compound medicine of synergistic treatment goat.
Another object of the present invention has provided the preparation method of this medicine and the application of treatment goat thereof.
Medicine of the present invention contains Caulis Sinomenii, Rhizoma Atractylodis (parched), and Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati and Dioscorea septemloba Thunb. , these drug regimens make the effect of each medicine produce synergism, thereby can effectively treat goat.Caulis Sinomenii in the side, bitter, hot, flat, return liver, spleen channel.Have the function of expelling wind and removing dampness, removing obstruction in the collateral to relieve pain, diuresis, be used for rheumatic arthralgia, diseases such as arthroncus.These flavour of a drug hardship, can dampness heat clearing away, kind again dispeling the wind, right wind energy wins wet, is intended to dehumidifying herein, wetly dispels that then heat is lonely, and " the temmoku Rhizoma Dioscoreae is planted will in Zhejiang " carries it and have the length of " row water diuresis, rush down part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels blood system damp and hot ".Damp and hot going with the wind separated from urine, then swell and ache from disappearing.As seen the damp eliminating of Caulis Sinomenii tool, heat clearing away, analgesic effect, thus reuse think monarch.Rhizoma Atractylodis, hot, bitter, warm, return spleen, stomach, Liver Channel, function is drying damp and strengthening spleen, and expelling wind and cold is used for rheumatic arthralgia, the beriberi edema.Cortex Phellodendri, bitter, cold, return kidney, urinary bladder channel.Heat clearing and damp drying is arranged, and the function of skin ulcer is treated in detoxifcation, with Rhizoma Atractylodis mutually 5, cures mainly damp and hot migratory arthralgia caused by wind, diseases such as bones and muscles pain.Cortex Phellodendri, the same usefulness of Rhizoma Atractylodis (parched) two medicines, Jiao's wet source in can breaking, the turbid damp of the sharp part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels accumulates for a long time damp and hot clearly, and to help the power of Sinomenium acutum, shared is minister.Herba Leonuri, bitter, hot, be slightly cold, return lung, liver, pericardium channel, have blood circulation promoting and blood stasis dispelling, the effect of inducing diuresis to remove edema.Rhizoma Polygoni Cuspidati, is slightly cold at little hardship, returns liver, gallbladder, lung meridian, and expelling wind and removing dampness is arranged, and the function of dissipating blood stasis analgesic therapy is used for arthralgia, jaundice due to damp-heat, carbuncle sore tumefacting virus.Dioscorea septemloba Thunb. , bitter, flat, return kidney, stomach warp, the function dampness removing is dispelled turbid, the blood stasis dispelling that dispels the wind, but activating blood and promoting diuresis, eliminating stasis to stop pain, clearing heat for detumescence is so think assistant.
Medicine of the present invention also contains Fructus Aurantii Immaturus (parched), Radix Salviae Miltiorrhizae, Spina Gleditsiae except that containing said components.Fructus Aurantii Immaturus (parched), bitter cold is held concurrently sour, returns spleen, stomach warp, function heat clearing away removing food stagnancy, the diffusing painful abdominal mass of reducing phlegm.Be used for damp and hot stagnant.This product and Rhizoma Atractylodis are all returned the taste warp, can dry spleen damp eliminating, and circulation of qi promoting stream is wet, makes that the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels is wetly to be difficult to gather.Radix Salviae Miltiorrhizae, bitter, be slightly cold, GUIXIN, Liver Channel, the function promoting blood circulation to restore menstrual flow, stasis-dispelling and pain-killing is used for pyretic arthralgia disease such as swell and ache.Fructus Aurantii Immaturus (parched), Radix Salviae Miltiorrhizae two flavor medicines and Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. are public, can strengthen the effect of removing damp and stopping pain, make adjuvant drug altogether.Spina Gleditsiae, hot, warm, return liver, stomach warp, function is sensible, expectorant, burst hard, can promoting the circulation of QI and reducing phlegm, again can activating blood circulation and dissipating blood stasis, arrogate to oneself and attack pyretic toxicity and through sick institute, available for making.More than all medicines, according to the rationale of science of TCM formulas, ingenious compatibility, clearing heat and expelling damp is played in tight combination altogether, promoting blood circulation to remove obstruction in the collateral, the merit of reducing swelling and alleviating pain stagnates to such an extent that remove thereby reach the stasis of blood, and is damp and hot clearly, gout controlled, thereby reached to the purpose that heals.
The consumption of drug component of the present invention is groped to sum up to draw through the inventor in a large number, and each amounts of components all has curative effect preferably in following ranges, and medicine of the present invention contains
Caulis Sinomenii 331-993 part Rhizoma Atractylodis (parched) 165-497 part Cortex Phellodendri 110-330 part,
Herba Leonuri 165-497 part Rhizoma Polygoni Cuspidati 165-497 part Dioscorea septemloba Thunb. 165-497 part.
Preferable range is:
Caulis Sinomenii 497-828 part Rhizoma Atractylodis (parched) 248-414 part Cortex Phellodendri 165-275 part,
Herba Leonuri 248-414 part Rhizoma Polygoni Cuspidati 248-414 part Dioscorea septemloba Thunb. 165-497 part.
The best is:
220 parts of 331 portions of Cortex Phellodendris of 662 parts of Rhizoma Atractylodis (parched) of Caulis Sinomenii,
331 parts of 331 parts of Dioscorea septemloba Thunb. of 331 parts of Rhizoma Polygoni Cuspidati of Herba Leonuri.
Medicine of the present invention also contains Fructus Aurantii Immaturus, Radix Salviae Miltiorrhizae, Spina Gleditsiae, and each amounts of components can be:
Caulis Sinomenii 331-993 part Rhizoma Atractylodis (parched) 165-497 part Cortex Phellodendri 110-330 part,
Herba Leonuri 165-497 part Rhizoma Polygoni Cuspidati 165-497 part Dioscorea septemloba Thunb. 165-497 part,
Radix Salviae Miltiorrhizae 132-398 part Fructus Aurantii Immaturus (parched) 132-398 part Spina Gleditsiae 132-398 part.
Preferable range is:
Caulis Sinomenii 497-828 part Rhizoma Atractylodis (parched) 248-414 part Cortex Phellodendri 165-275 part,
Herba Leonuri 248-414 part Rhizoma Polygoni Cuspidati 248-414 part Dioscorea septemloba Thunb. 248-414 part,
Radix Salviae Miltiorrhizae 198-331 part Fructus Aurantii Immaturus (parched) 198-331 part Spina Gleditsiae 198-331 part.
The best is:
220 parts of 331 portions of Cortex Phellodendris of 662 parts of Rhizoma Atractylodis (parched) of Caulis Sinomenii,
331 parts of 331 parts of Spina Gleditsiaes of 331 parts of Rhizoma Polygoni Cuspidati of Herba Leonuri,
265 parts of 265 parts of Dioscorea septemloba Thunb. of 265 parts of Fructus Aurantii Immaturus (parched) of Radix Salviae Miltiorrhizae.
Medicine of the present invention can adopt the Chinese medicine preparation conventional method to be prepared into any pharmaceutically useful conventional formulation.For example these crude drug pulverize mix homogeneously can be made powder takes after mixing it with water; Also can be with these medicines decocting together, the condensed water decocting liquid is made oral liquid then; But, preferably crude drug Caulis Sinomenii, Radix Salviae Miltiorrhizae are carried out special extraction such as ethanol extraction, but this can not limit protection scope of the present invention in order to make each crude drug of this medicine better bring into play drug effect.
The core group of medicine of the present invention is divided into Caulis Sinomenii, Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. , also contain some other components, other components can be replaced by the suitable Chinese medicine with the identical property of medicine, effect individually or simultaneously, its drug effect of Chinese medicine preparation after the replacement and effect are constant, and preferably other components are Spina Gleditsiae, Radix Salviae Miltiorrhizae, Fructus Aurantii Immaturus (parched).
Medicine of the present invention can prepare by the following method:
(a) Caulis Sinomenii of described weight proportion is carried out reflux, extract, twice with the ethanol of 70%-95%, merges extracted twice liquid, reclaim ethanol, 45-55 ℃ dry down, dry extract 1;
(b) Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati and the Dioscorea septemloba Thunb. of described weight proportion and the residue after the Caulis Sinomenii alcohol extraction are decocted with water twice, add water serves as easy not have powder at every turn, merge decocting liquid twice, filter to such an extent that consider liquid 2, concentrate considering liquid, to become proportion be 1.25～1.35 extractum, dry under 45-55 ℃, get dry extract 2;
(c) merge dry extract 1 and 2, add the extract extractum of other compositions, it is ground into powder,, the active component of preparation cost invention medicine;
(d) with gained active component and correctives, the abundant mixing of adjuvant, make any medicament of pharmaceutics.For the preferred medicine of making by Caulis Sinomenii, Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Spina Gleditsiae, Radix Salviae Miltiorrhizae, Fructus Aurantii Immaturus (parched), Dioscorea septemloba Thunb. of the present invention, can prepare by the following method:
(a) Caulis Sinomenii, the Radix Salviae Miltiorrhizae with described weight proportion carries out reflux, extract, twice with the ethanol of 70%-95%, merges extracted twice liquid, reclaims ethanol, and be dry under 45-55 ℃, gets dry extract 1;
(b) Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Spina Gleditsiae, Fructus Aurantii Immaturus (parched), Dioscorea septemloba Thunb. and the Caulis Sinomenii of described weight proportion, the residue after the Radix Salviae Miltiorrhizae alcohol extraction are decocted with water twice, add water serves as easy not have powder at every turn, merge decocting liquid twice, filter to such an extent that consider liquid 2, concentrate considering liquid, to become proportion be 1.25～1.35 extractum, dry under 45-55 ℃, get dry extract 2;
(c) merge dry extract 1 and 2, it is ground into powder, the active component of preparation cost invention medicine;
(d) with gained active component and correctives, the abundant mixing of adjuvant, make any medicament of pharmaceutics.In the above-mentioned preparation method, when in the preferred steps (a) medicine being added ethanol extraction, adding amount of alcohol for the first time is 12 times, extracts 1 hour, and add amount of alcohol the second time is 10 times, extracts 1 hour.(b) during with the medicine extracting in water, add 10 times in water for the first time in, decocted 2 hours, add 8 times in water the second time, decocted 1.5 hours.
More than during each forms, weight is calculated with crude drug, in the present invention, if weight is unit with the gram, this composition can be made into 1000 doses of pharmaceutical preparatioies, described 1000 doses of fingers, 1000 doses in the final drug preparation of making, as make 1000 in 1000 of capsule preparations, 1000 in tablet, granule 1000g, oral liquid 1000 peace bottles, injection 1000 peace bottles, drop pill etc., also can make big packing as granule, as the 100-500 bag, specifically can be 100 bags, 150 bags, 200 bags, 250 bags, 500 bags, every bag can be used as dose 1 time.
More than form,, can be made into the taking dose preparation 50-1000 time, as tablet if be unit with the gram, make 1000, each taking dose can be the 1-20 sheet, can take 50-1000 time altogether, as granule, make 200 bags, take the 1-2 bag at every turn, can take 100-200 time altogether.
More than form as proportioning according to weight portion, when producing, can increase or reduce according to corresponding ratio, as large-scale production can be unit with kilogram or with the ton, small-scale production can be unit with the gram also, weight can increase or reduce, but the raw medicinal herbs weight proportion constant rate between each composition.
More than each single medicinal material, especially adjuvant drug, messenger drug or adjuvant drug and messenger drug in forming, can be replaced by suitable Chinese medicine individually or simultaneously with the identical property of medicine, effect, it is constant to replace back its drug effect of Chinese medicine preparation.
The active component of medicine of the present invention can add various conventional adjuvant required when preparing different dosage form, be prepared into any common formulations as disintegrating agent, lubricant, binding agent etc. with the method for Chinese medicinal of routine, as tablet, powder, capsule, granule, drop pill, intramuscular injection agent, instillation, unguentum, ointment, plaster etc.
The present invention be at distinguished veteran doctors of TCM on the basis of proved recipe, through a large amount of preparation technology's test and pharmacology, the resulting sugar-free preparation of pharmacodynamics test.Pain relieving of the present invention is rapid, and is evident in efficacy, has clearing heat and expelling damp, dredging the meridian, the drainage of reducing swelling and alleviating pain promotion blood uric acid, and prevention joint deformity and nephropathy prevent tophaceous formation, are applicable to all kinds of goat patients.
Advantage of the present invention is a reasonable recipe, poisonous side effect of medicine is low, and the toxic and side effects that has overcome Western medicine in the past is big; Treatment by Chinese herbs goat curative effect worm medicine low or commonly used, animal drugs, the plant amedica that toxicity is stronger, the shortcoming of medicated wine.Said preparation also has prevention and therapeutical effect to the complication that gout caused in addition.Because the present invention is the sugar-free preparation, can be used for treating gout complication with diabetes patient.
For a better understanding of the present invention, below further set forth the beneficial effect of invention medicine by pharmacodynamics test, experimental example comprise medicine of the present invention (hereinafter referred to as: but ache the wind granule) pharmacodynamics test.But test is intended to further specify the particulate effect of wind of aching below, but not limitation of the present invention.
Pharmacodynamic test of active extract of the present invention:
One, test objective: but the particulate prescription of the wind of aching is made up of Chinese medicines such as Caulis Sinomenii, Cortex Phellodendris.Effect with promoting blood circulation to remove obstruction in the collateral, clearing away heat-damp and promoting diuresis, reducing swelling and alleviating pain cures mainly arthromyodynias such as gout, rheumatoid arthritis.In order to verify the curative effect of this medicine, we cure mainly according to function, have carried out Pharmacodynamic test of active extract such as antiinflammatory, analgesia and gout.
Two, test material
1, be subjected to the reagent thing: but the wind granule of aching is provided by Tianshili New Resource Pharmaceutical Co Ltd, Tianjin, the suitable 5.0g crude drug of 1g medicated powder.Adding distil water is mixed with certain density suspension before the test, for the animal gastric infusion.
2, laboratory animal: KM kind mice, body weight 20～25g; Wistar kind rat, body weight 180～300g; Be male.White big ear rabbit, body weight 2.1～2.5kg, male and female dual-purpose.Provide (Tianjin moving 001) by the production of Tianjin Inst. of Materia Medica animal housing, raise in the observation ward of central air-conditioning, the piece material that food Nat'l Pharmaceutical ﹠ Biological Products Control Institute animal center is produced is drunk tap water.
3, uric acid sodium: crystallite uric acid sodium, provide by synthetic two Room of Tianjin Inst. of Materia Medica, precision takes by weighing uric acid sodium medicated powder before the test, elder generation's porphyrize in mortar, add normal saline then and be mixed with the suspension that concentration is 100mg/ml, for animal subcutaneous injection or injections in articular cavity, induce the generation of gouty arthritis.
Three, test method and result
(1), the arthritic effect of gout
1, to the bullate influence of uric acid sodium inducing mouse foot
Test is divided into 5 groups, 10 every group at random with 50 mices.Every day, gastric infusion was 1 time, successive administration 4 days.After the last administration 30 minutes,, induce the generation of gouty arthritis at the right back sufficient pad subcutaneous injection uric acid sodium normal saline suspension 0.05ml of portion.Respectively cause scorching before and cause scorching back 1,2,4 and 6 hour, survey with projector (amplifying 6 times) and cause 0.5cm place diameter under the scorching limb ankle joint, consequently the difference of inflammation front and back is made the arthritic swelling degree of gouty.Average and the matched group of getting each administration group compare, and carry out statistical test.Result's (seeing Table 1) shows, but aches the antiinflammatory action highly significant of wind granule to the mice gouty arthritis.
The wind granule is to the influence of mice gouty arthritis but table 1. is ached
Normal value uric acid sodium cause the swollen degree of scorching metapedes (Δ X ± SD, cm)
Grouping
(X ± SD, cm) 1 hour 2 hours 4 hours 6 hours
Contrast 2.30 ± 0.19 0.29 ± 0.12 0.42 ± 0.20 0.94 ± 0.44 1.06 ± 0.53
Indometacin 0.005g/kg 2.55 ± 0.11 0.08 ± 0.12
* *0.18 ± 0.12
* *0.36 ± 0.11
* *0.46 ± 0.12
But ache wind 20g crude drug/kg 2.34 ± 0.15 0.10 ± 0.11
*0.14 ± 0.15
*0.48 ± 0.34
*0.68 ± 0.28
But ache wind 10g crude drug/kg 2.36 ± 0.16 0.05 ± 0.07
* *0.20 ± 0.18
*0.59 ± 0.29
*0.88 ± 0.34
But the 5g crude drug/kg 2.42 that aches ± 0.17 0.04 ± 0.07
* *0.10 ± 0.12
*0.39 ± 0.18
*0.67 ± 0.26
*P＜0.05?
**P＜0.01?
***P＜0.001
2. uric acid sodium is induced the bullate influence of rat foot
Test is divided into 5 groups, 10 every group at random with 50 rats.Every day, gastric infusion was 1 time, successive administration 4 days.After the last administration 30 minutes,, induce the generation of rat acute gouty arthritis at every right back sufficient pad subcutaneous injection uric acid sodium suspension 0.05ml of portion of rat.Cause scorching before and cause scorching afterwards 1,2,4,6 and 8 hour, use projector (amplifying 6 times) to survey the diameter that causes 0.5cm place under the scorching limb ankle joint respectively, so that the difference before and after scorching is as the swelling degree of rat gouty arthritis, and average and the matched group of getting each administration group compare, and carry out statistical test.Result's (seeing Table 2) shows, but the wind granule of aching shows as strong and effective acting time is long to the bullate inhibitory action of rat foot due to the uric acid sodium to the antiinflammatory action highly significant of rat gouty arthritis.
The wind granule is to the influence of rat gouty arthritis but table 2. is ached
Normal value uric acid sodium cause the swollen degree of scorching back rat foot (Δ X ± SD, cm)
Grouping
(X ± SD, cm) 1 hour 2 hours 4 hours 6 hours 8 hours
Matched group 5.72 ± 0.29 0.71 ± 0.24 0.69 ± 0.17 0.81 ± 0.19 0.87 ± 0.28 1.07 ± 0.36
Indometacin
5.84±0.22 0.35±0.19
** 0.37±0.22
**?0.26±0.15
***?0.61±0.23
** 0.61±0.31
*
0.01g/kg
Press down granulates
6.04±0.15 0.14±0.11
***?0.18±0.17
***?0.29±0.20
***?0.54±0.37
** 0.73±0.39
20g crude drug/kg
Press down granulates
6.02±0.23 0.14±0.19
***?0.170.17
*** 0.24±0.15
*** 0.57±0.29
* 0.61±0.30
**
10g crude drug/kg
Press down granulates
6.18±0.12 0.06±0.07
***?0.12±0.16
**?0.23±0.18
*** 0.28±0.29
*** 0.35±0.24
***
5g crude drug/kg
*P＜0.05?
**P＜0.01
***?P＜0.001
3. uric acid sodium is induced the influence of rabbit acute arthritis
Test is divided into 5 groups, 5 every group at random with 25 rabbit.But ache wind granule gastric infusion 3 days, every day 1 time.Fixing rabbit after the 3rd administration, knee joint intracavitary administration uric acid sodium suspension 0.6ml causes inflammation to the right.Put to death rabbit in 5 hours behind the Yu Zhiyan, collect joint fluid, get 20 μ l by the numeration of leukocyte method and carry out numeration of leukocyte, get synovial membrane and be placed in the formalin fixingly, make paraffin section, carry out histological examination.Result's (seeing Table 3) shows, but leukocyte significantly is lower than model control group (p＜0.01) in the wind granule 10g crude drug of aching/kg group rabbit joint fluid.
The wind granule is to the influence of rabbit acute arthritis but table 3. is ached
Neutral grain in the dosage number of animals synovial membrane
Grouping leukocyte * 10
9/ L
((only) number of cells/grid of g crude drug/kg)
Normal control group 0500
Model control group 05 30.74 ± 22.41 81.6 ± 53.8
Colchicine 0.67mg/kg 5 1.60 ± 1.50** 23.4 ± 8.0*
But ache wind granule 10 5 2.40 ± 2.60** 22.6 ± 11.9*
But the wind granule 55 17.60 of aching ± 14.70 73.0 ± 19.5
*P＜0.05?
**P＜0.01
Histological examination is the result show, normal group man rabbit knee synovial membrane internal layer is by multiple 1-3 layer flat epithelial cell, i.e. synovial cell, and synovial membrane inner tissue structure is normal, does not see neutrophilic infiltration.The hyperemia of model control group rabbit synovial membrane, the synovial cell is that kitchen range comes off, necrosis, visible more neutrophilic infiltration in the synovial membrane.Colchicine group rabbit has the synovial cell of more complete arrangement, and a small amount of neutrophilic infiltration is arranged in the synovial membrane.But the wind granule 10g crude drug of aching/kg group rabbit synovial cell is more complete, and a small amount of neutrophilic infiltration is arranged in the synovial membrane.It is similar to model control group that but the wind granule 5g crude drug of aching/kg group rabbit synovial tissue changes, but synovial cell's damage slightly gentlier.Each test group animal has the infiltration of neutrophilic leukocyte to calculate (observation of 15 * 40 power microscopes) with the net form micrometer in the synovial membrane, the results are shown in Table 3.As seen but 10g crude drug/kg wind granule of aching is similar to the colchicine group, can obviously reduce neutrophilic leukocyte in intrasynovial infiltration, shows that this medicine has very significant protective effect to the rabbit synovium of joint.
(2), analgesic activity
1. the influence that mouse writhing is reacted
Test is divided into 5 groups, 10 every group at random with 50 mices.Every day, gastric infusion was 1 time, successive administration 3 days.After the last administration 30 minutes, give that injection concentration is that 0.6% acetic acid 0.2ml/ only causes pain in the mouse peritoneal, after 10 minutes with the writhing response number of times of the 2nd mice in 10 minutes behind the enumerator record medicine.What calculate each treated animal on average turns round the body number, with matched group relatively, carry out statistical test, result's (seeing Table 4) shows that reaction all has significant inhibitory effect but 3 dosage of wind granule of aching are to the mice acetic acid twisting, analgesic activity is stronger.
The wind granule is to the influence of mouse writhing reaction but table 4. is ached
(number of animals (only) of g crude drug/kg) is turned round body number (X ± SD) to the grouping dosage
Matched group 0 10 40.3 ± 14.5
Indometacin 0.01g/kg 10 13.2 ± 5.9
* *
But ache wind granule 20 10 20.4 ± 5.5
* *
But ache wind granule 10 10 16.6 ± 12.2
* *
But ache wind granule 5 10 24.6 ± 9.5
*
*P＜0.05?
**P＜0.01?
***P＜0.001
2. the influence that the mice whipping is reacted
Test is divided into 4 groups, 10 every group at random with 40 mices.Every day, gastric infusion was 1 time, successive administration 3 days.After the last administration 0.5,1.0,2.0 and 3.0 hour, respectively mouse tail tip 1-2cm is put into 55 ± 0.5 ℃ of waters bath with thermostatic control, survey the TFL of mice with goldspink board quartz electronic stopwatch, as threshold of pain index.Average and the matched group of getting each group compare, and carry out statistical test, and result's (seeing Table 5) shows that to the caused mice pain reaction of warm stimulation, but 2 dosage of wind granule of aching all have the obvious suppression effect.
The wind granule is to the influence of mice whipping reaction but table 5. is ached
Pain threshold after the dosage administration (X ± SD, s)
Grouping
(g crude drug/kg) 0.5 hour 1.0 hours 2.0 hours 3.0 hours
Matched group 0 1.44 ± 0.39 1.38 ± 0.25 1.38 ± 0.22 1.40 ± 0.15
Indometacin 0.005g/kg 2.05 ± 0.38
*1.82 ± 0.70 1.85 ± 0.42
*1.77 ± 0.38
*
But ache wind granule 20 2.15 ± 0.33
* *2.22 ± 0.27
* *2.14 ± 0.30
*2.10 ± 0.36
*
But ache wind granule 10 2.22 ± 0.56
*2.36 ± 0.59
* *2.14 ± 0.84
*2.41 ± 0.80
*
*P＜0.05?
**P＜0.01?
***P＜0.001
3. the influence that the rat whipping is reacted
Test is divided into 5 groups, 10 every group at random with 50 rats.Irritate stomach every day 1 time, successive administration 3 days.After the last administration 0.5,1.0,2.0,3.0 and 4.0 hour, rat tail tip 2～3cm is put into 55 ± 0.5 ℃ of waters bath with thermostatic control, the whipping response latency of surveying rat with goldspink board quartz electronic stopwatch is as pain threshold.Average and the matched group of getting each group relatively carry out statistical test, and result's (seeing Table 6) shows, but ache the administration of wind particulate oral, the incubation period of can prolong rats water-bath whipping reacting, have the very characteristics of significant analgesia role and long action time.
The wind granule is to the influence of rat whipping reaction but table 6 is ached
Pain threshold after the administration (X ± SD, second)
Grouping
0.5 hours 1.0 hours 2.0 hours 3.0 hours 4.0 hours
Matched group 2.29 ± 0.56 2.69 ± 0.48 2.67 ± 0.67 2.65 ± 1.21 2.51 ± 0.67
Indometacin 0.005g/kg 4.03 ± 0.54
* *4.88 ± 1.45
* *2.98 ± 0.28 3.24 ± 0.79 2.99 ± 0.29
But ache wind granule 20g crude drug/kg 5.00 ± 1.11
* *4.85 ± 1.35
* *4.50 ± 0.78
* *4.29 ± 1.35
*3.96 ± 1.03
*
But ache wind granule 10g crude drug/kg 4.40 ± 0.75
* *4.09 ± 0.85
* *3.39 ± 0.46
*3.26 ± 0.43 3.20 ± 0.36
*
But ache wind granule 5g crude drug/kg 3.85 ± 0.62
* *3.50 ± 0.53
*3.27 ± 0.45
*3.48 ± 0.52 2.44 ± 0.36
*P＜0.05?
**P＜0.01?
***P＜0.001
(3), antiinflammatory action
1. to the bullate influence of mouse ear
Test is divided into 5 groups, 10 every group at random with 50 mices.Every day, gastric infusion was 1 time, successive administration 4 days.After the last administration 30 minutes, drip Oleum Tiglii proinflammatory agent 0.05ml to every mice left side auricular concha.Put to death mice in causing scorching 2 hours, cut left and right sides auricle, lay same position auricle, weigh with diameter 9mm card punch, with left and right sides auricle weight difference as Mus dropsy of ear degree.Average and the matched group of getting each group compare, and carry out statistical test, and result's (seeing Table 7) shows, but the bullate inhibitory action highly significant of mouse ear that the wind granule of aching causes Oleum Tiglii increases antiinflammatory action with dosage and strengthens.
The wind granule is to the bullate influence of mouse ear but table 7. is ached
The grouping dosage (animal (only) ear of g crude drug/kg) swell (Δ X ± SD, mg)
Matched group 0 10 12.95 ± 4.21
Indometacin 0.005g/kg 10 7.94 ± 3.32
*
But ache wind granule 20 10 5.35 ± 3.28
* *
But ache wind granule 10 10 6.35 ± 2.27
*
But ache wind granule 5 10 8.80 ± 4.26
*
*P＜0.05?
**P＜0.01?
***P＜0.001
2. to the bullate influence of rat foot
Test is divided into 5 groups, 10 every group at random with 50 rats.Every day, gastric infusion was 1 time, successive administration 3 days.After the last administration 30 minutes, cause inflammation to every rat right hind leg foot mind-set ankle joint direction subcutaneous injection 1% carrageenin 0.05ml.Cause scorching back 1,2,3 and 4 hour, the diameter (amplifying 6 times) with 0.5cm place under the projector measurement right hind ankle joint deducts normal value, as the swollen degree of rat foot.Take the mean and the matched group comparison, carry out statistical test, result's (seeing Table 8) shows, but 3 dosage of wind granule of aching are all very obvious to the bullate inhibitory action of rat carrageenan foot.
The wind granule is to the bullate influence of rat foot but table 8. is ached
Normal value cause the swollen degree of scorching metapedes (Δ X ± SD, cm)
Grouping
(X±SD，cm)
1 hour 2 hours 3 hours 4 hours
Matched group 0 7.49 ± 0.18 1.30 ± 0.62 2.11 ± 0.48 2.58 ± 0.63 2.19 ± 0.42
Indometacin 0.01g/kg 7.43 ± 0.24 0.30 ± 0.18
* *0.80 ± 0.35
* *1.68 ± 0.30
*2.06 ± 0.28
But ache wind granule 20g crude drug/kg 7.25 ± 0.24 0.68 ± 0.31
*1.35 ± 0.41
*1.86 ± 0.50
*1.95 ± 0.29
But ache wind granule 10g crude drug/kg 7.26 ± 0.21 1.00 ± 0.35 1.56 ± 0.44
*1.55 ± 0.52
*1.67 ± 0.55
*
But ache wind granule 5g crude drug/kg 7.22 ± 0.21 0.52 ± 0.25
*1.12 ± 0.47
*1.93 ± 0.26
*1.93 ± 0.39
*P＜0.05?
**P＜0.01?
***P＜0.001
3. to the bullate influence of rat granuloma
Laboratory animal and divide into groups the samely, every day, gastric infusion was 1 time, successive administration 10 days, matched group is given distilled water.After the 3rd administration, under the ether light anaesthesia, rat back is shaved hair, sterilization, incision skin, it is subcutaneous that 30mg sterilization cotton balls is imbedded the both sides armpit.Put to death rat the 11st day morning, strips out the granuloma induced by implantation of cotton pellets thing, and materials such as the fat that place to go outside is adhered to, connective tissue claim weight in wet base, and then 60～70 ℃ of oven dry, the scale dry weight deducts the weight of cotton balls, and it is heavy to be granuloma.Get the heavy average of each administration group granuloma and matched group relatively, carry out statistical test, result's (seeing Table 9) shows, but the wind granule of aching swells to rat granuloma the comparison significant inhibitory effect is also arranged.
The wind granule is to the bullate influence of rat granuloma but table 9. is ached
Dosage animal granuloma induced by implantation of cotton pellets (X ± SD, mg)
Grouping
G crude drug/kg (only)
The weight in wet base dry weight
Matched group 0 10 140.82 ± 29.12 29.60 ± 8.89
Cortisone 0.02g/kg 10 116.25 ± 9.99
*20.93 ± 5.91
*
But ache wind granule 20 10 103.20 ± 14.42
*18.00 ± 3.13
*
But ache wind granule 10 10 111.58 ± 15.83
*20.78 ± 3.31
*
But the wind granule 5 10 159.25 of aching ± 43.26 27.88 ± 7.92
*P＜0.05?
**P＜0.01?
***P＜0.001
4. to the influence of rat assist agent arthritis
Test is divided into 5 groups, 10 every group at random with 50 rats.The preparation system of adjuvant joins the bacillus calmette-guerin vaccine of deactivation in advance in the Freund of sterilization, grinds and is mixed with the adjuvant that concentration is 10mg/ml.The right back sufficient pad intradermal injection 0.1ml of the portion adjuvant every rat is then adopted in the model preparation, induces arthritic generation.
(1) to the influence of constitutional pathological changes: test beginning administration in preceding 3 days, irritate stomach every day 1 time, after the last administration, cause inflammation in 30 minutes, observe causing the swollen degree of scorching back adjuvant injection in 3,6,18 and 24 hours parapodum, adopt projector to survey 0.5cm place diameter (amplifying 6 times) under the rat right hind leg ankle joint.So that the difference before and after scorching is as the swelling degree, meansigma methods and the matched group of getting each administration group compare, carry out statistical test, result's (seeing Table 10) shows, but the wind granule of aching is to the inhibitory action highly significant of rat assist agent arthritis constitutional pathological changes, and its antiinflammatory action is rapid-action and acting duration is long.
The wind granule is to the influence of rat assist agent arthritis constitutional pathological changes but table 10. is ached
The swelling degree of normal value constitutional pathological changes (Δ X ± SD, cm)
Grouping
(X±SD，cm)
3 hours 6 hours 18 hours 24 hours
Matched group 0 5.65 ± 0.24 1.49 ± 0.34 1.69 ± 0.25 1.66 ± 0.26 1.38 ± 0.24
Indometacin 0.005mg/kg 5.49 ± 0.23 1.41 ± 0.39 1.52 ± 0.43 1.31 ± 0.12
*0.93 ± 0.38
*
But ache wind granule 20g crude drug/kg 5.76 ± 0.39 0.77 ± 0.37
*0.91 ± 0.46
*0.83 ± 0.47
* *0.40 ± 0.45
* *
But ache wind granule 10g crude drug/kg 5.92 ± 0.36 0.74 ± 0.46
*0.78 ± 0.58
* *0.62 ± 0.37
* *0.50 ± 0.57
*
But ache wind granule 5g crude drug/kg 5.82 ± 0.34 1.08 ± 0.42
*1.31 ± 0.75
*0.98 ± 0.48
*0.84 ± 0.33
*
*P＜0.05?
**P＜0.01?
***P＜0.001
(2) to the influence of Secondary cases pathological changes: the 8th day beginning successive administration after the adjuvant injection, every day, gastric infusion was 1 time, and dosage is the same, up to the 30th day, but observes the influence of wind granule to the Secondary cases pathological changes of aching.After surveying normal value on the 8th day, surveyed the left back vola thickness of rat (adjuvant injection offside every 4 days with micrometer, represent with mm), with the difference of itself and normal value index as Secondary cases pathological changes swelling degree, average and the matched group of getting each administration group compare, carry out statistical test, the results are shown among the table 11.The wind granule alleviates the adjuvant injection to the bullate effect highly significant of parapodum but as seen the result from table aches, and points out this medicine can alleviate the generation of rat assist agent arthritis Secondary cases pathological changes.
The wind granule is to the influence of Secondary cases pathological changes but table 11. is ached
The swelling degree of normal value Secondary cases pathological changes (Δ X ± SD, mm)
Grouping
(X±SD，
12 days 16 days 20 days 24 days 30 days
mm)
Matched group 5.02 ± 0.30 0.72 ± 0.44 0.72 ± 0.45 0.75 ± 0.39 0.78 ± 0.37 0.83 ± 0.30
Indometacin
5.34±0.22 0.30±0.32
* 0.30±0.14
** 0.52±0.35 0.52±0.18 0.33±0.35
**
0.005g/kg
But ache wind
5.34±0.24 0.33±0.21
* 0.23±0.25
** 0.28±0.31
** 0.16±0.23
*** 0.25±0.25
***
20g crude drug/kg
But ache wind
5.19±0.16 0.24±0.16
** 0.20±0.21
** 0.37±0.31
* 0.22±0.20
*** 0.42±0.28
**
10g crude drug/kg
But ache wind
5.28±0.23 0.25±0.18
* 0.20±0.16
** 0.20±0.13
** 0.17±0.15
*** 0.27±0.25
***
5g crude drug/kg
*P＜0.05?
**P＜0.01?
***P＜0.001
(3) to the influence of general pathological changes: except that the swelling degree of observing left back foot (adjuvant injection offside) as the arthritic index of Secondary cases, also observe general pathological changes such as the incidence rate of forelimb, ear, tail pathological changes and the order of severity thereof, press the record of 0-4 level point system, take the mean and carry out statistical test, result's (seeing Table 12) shows, heavy dose of (generation (p＜0.05) that the wind granule can alleviate the general pathological changes but 20g crude drug/kg) is ached, in, low dosage is the same with indometacin that the trend (p＞0.05) that alleviates the general pathological changes arranged.
The wind granule is to the influence of rat assist agent arthritis whole body pathological changes but table 12. is ached
The scoring of dosage animal morbidity animal whole body pathological changes
Grouping
((only) (only) of g crude drug/kg) (X ± SD)
Matched group 0 10 7 1.00 ± 0.942
Indometacin 0.005g/kg 10 5 0.50 ± 0.53
But ache wind granule 20 10 2 0.20 ± 0.42
*
But ache wind granule 10 10 5 0.80 ± 0.92
But ache wind granule 5 10 5 0.50 ± 0.53
*P＜0.05
(4), the influence of pair cell immunity:
Test is divided into 5 groups, 10 every group at random with 50 mices.Continuously gastric infusion is 3 days, at 2 of the nape portion subcutaneous injection 7% of every mice, 4-dinitrochlorobenzene acetone soln 20 μ l sensitization, but and then ache wind granule 11 days of continuous oral, control group administered physiological saline.After the last administration, be coated with 2 at every mice left side auricular concha, 4-dinitrochlorobenzene acetone soln 20 μ l attack, put to death animal after 16 hours, lay left and right sides auricle with the card punch of diameter 9mm, weigh with torsion balance, represent the swelling degree with left and right sides auricle weight difference, as the index of delayed allergy (DTH).Average and the matched group of getting each administration group compare, and carry out statistical test, and result's (seeing Table 13) shows that increment has the obvious suppression effect but the wind granule 20g crude drug/kg that aches is to the T lymphocyte, and the effect of tangible antiallergic action is promptly arranged.
The wind granule is to the influence of the inductive mouse DTH reaction of DNCB but table 13. is ached
Dosage number of animals ear swelling degree
Grouping
(g crude drug/kg) (only) (Δ X ± SD, mg)
Matched group 0 10 30.0 ± 4.8
Hydrocortisone 0.02g/kg 10 25.2 ± 4.6
*
But ache wind granule 20 10 19.8 ± 6.3
*
But ache wind granule 10 10 26.0 ± 5.0
But ache wind granule 5 10 26.4 ± 5.0
*P＜0.05?
**P＜0.01
(5), to the microcirculation of mouse auricle effect
50 of 20-22g mices are selected in this test for use, are divided into 5 groups at random, and 10 every group, gastric infusion is 3 days continuously, and matched group gavages normal saline.After the last administration 40 minutes, inject 20% urethane 0.07ml/10g to mouse peritoneal, after the anesthesia mice abdomen crouched and be fixed on the observation platform, make auricle open and flat in the ear holder, a little liquid paraffin is dripped on the surface, put under 40 times of anatomical lens observation and respectively organize the open quantity of Mice Auricle blood capillary, with blood capillary and 1mm
2The intersection that the inner boundary blood vessel intersects is counted and is represented, take the mean and the matched group comparison, carry out the statistics test, result's (table 14) but after showing the oral wind granule 20g crude drug/kg that aches of mice, the open quantity of auricle blood capillary increases, and truly has to promote the microcirculation of mouse auricle effect under physiological status.
The wind granule is to the influence of auricle blood capillary opening amount but table 14. is ached
Dosage number of animals blood capillary
Group
(the g crude drug/kg) (only) intersection is counted
Matched group 0 10 3.9 ± 1.1
FUFANG DANSHEN DIWAN 2 10 7.8 ± 1.8
* *
But ache wind granule 20 10 5.0 ± 1.1
*
But ache wind granule 10 10 4.4 ± 1.1
But ache wind granule 5 10 4.2 ± 0.9
*P＜0.05?
***P＜0.001
Four, conclusion (of pressure testing)
Experimental result proves, but aches the wind granule under 5g crude drug/kg, 10g crude drug/kg and 20g crude drug/kg dosage, has the anti-inflammatory and analgesic effect and the arthritic effect of gout of highly significant.Show as wherein that the rat foot swollen to Oleum Tiglii induced mice ear, that carrageenin causes is swollen, rat granuloma is swollen and inhibitory action rat assist agent arthritis is stronger, especially to the antiinflammatory action highly significant of chronic inflammatory disease (rat granuloma swollen), and present certain dose-dependence, in similar Chinese medicine, seldom see.To the analgesic activity of mice chemical stimulation, warm stimulation and the warm stimulation of rat also highly significant.Particularly to swollen by the inductive mice of uric acid sodium foot, the rat foot is swollen and the rabbit acute arthritis also has the inhibitory action of highly significant; show as and suppress swelling, reduce in the joint fluid leukocyte count and alleviate the lesion degree of synovial tissue, gouty arthritis is had significant protective effect.But the wind granule 20g crude drug/kg that aches can suppress the increment of T lymphocyte, and the effect of tangible antiallergic action is promptly arranged, and also can increase the blood capillary crossing net simultaneously and count, and improves auricular microcirculation, shows that this medicine has certain function of promoting blood circulation to disperse blood clots.
The wind granule has good effect in arthromyodynias such as treatment gout, rheumatoid arthritiss but this experimental result proof is ached.
The specific embodiment
Below further set forth the preparation method of medicine of the present invention by specific embodiment
Embodiment 1: the capsule preparation of medicine of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 497g Rhizoma Atractylodis (parched) 414g Cortex Phellodendri 165g,
Herba Leonuri 248g Rhizoma Polygoni Cuspidati 248g Dioscorea septemloba Thunb. 248g,
(1), Caulis Sinomenii is with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds and goes into steviosin, dextrin in right amount, and the snap fit capsule of packing into is made 1000 seed lac wafers.
Embodiment 2: medicinal granule preparation of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 662g Rhizoma Atractylodis (parched) 331g Cortex Phellodendri 220g,
Herba Leonuri 331g Rhizoma Polygoni Cuspidati 331g Dioscorea septemloba Thunb. 331g,
(1), Caulis Sinomenii is with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds ethanol and makes adhesive, adds appropriate amount of starch and makes filler, makes the 1000g granule.
Embodiment 3: medicinal granule preparation of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 993g Rhizoma Atractylodis (parched) 398g Cortex Phellodendri 330g,
Herba Leonuri 497g Rhizoma Polygoni Cuspidati 497g Dioscorea septemloba Thunb. 497g,
(1), Caulis Sinomenii is with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds ethanol and makes adhesive, adds starch and makes filler, makes the 1000g granule.
Embodiment 4: the granule preparation of medicine of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 662g Rhizoma Atractylodis (parched) 331g Cortex Phellodendri 220g,
Herba Leonuri 331g Rhizoma Polygoni Cuspidati 331g Spina Gleditsiae 265g,
Radix Salviae Miltiorrhizae 265g Fructus Aurantii Immaturus (parched) 265g Dioscorea septemloba Thunb. 331g,
(1), Caulis Sinomenii, Radix Salviae Miltiorrhizae be with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii, Radix Salviae Miltiorrhizae residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds ethanol and makes adhesive, adds starch and makes filler, makes the granule of 1000g.
Embodiment 5: the granule preparation of medicine of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 331g Rhizoma Atractylodis (parched) 165g Cortex Phellodendri 110g,
Herba Leonuri 165g Rhizoma Polygoni Cuspidati 165g Spina Gleditsiae 165g,
Radix Salviae Miltiorrhizae 132g Fructus Aurantii Immaturus (parched) 132g Dioscorea septemloba Thunb. 132g,
(1), Caulis Sinomenii, Radix Salviae Miltiorrhizae be with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii, Radix Salviae Miltiorrhizae residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds ethanol and makes adhesive, adds starch and makes filler, makes the 1000g granule.
Embodiment 6: the preparation tablets of medicine of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 828g Rhizoma Atractylodis (parched) 414g Cortex Phellodendri 275g,
Herba Leonuri 414g Rhizoma Polygoni Cuspidati 414g Spina Gleditsiae 331g,
Radix Salviae Miltiorrhizae 331g Fructus Aurantii Immaturus (parched) 331g Dioscorea septemloba Thunb. 331g,
(1), Caulis Sinomenii, Radix Salviae Miltiorrhizae be with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii, Radix Salviae Miltiorrhizae residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds ethanol and makes adhesive, adds adjuvant and makes granule, adds adhesive and makes 1000 tablets of tablets.
Embodiment 7: the granule preparation of medicine of the present invention
The raw material of Chinese medicine of getting selected following weight is standby through screening, clean, dry, pulverizing:
Caulis Sinomenii 662g Rhizoma Atractylodis (parched) 198g Cortex Phellodendri 165g,
Herba Leonuri 248g Rhizoma Polygoni Cuspidati 200g Spina Gleditsiae 180g,
Radix Salviae Miltiorrhizae 240g Fructus Aurantii Immaturus (parched) 140g Dioscorea septemloba Thunb. 1248g,
(1), Caulis Sinomenii, Radix Salviae Miltiorrhizae be with the ethanol extractions twice of 12 times of amounts and 8 times of amount 70%-95%, extraction time is 1.5 hours, merges twice and considers liquid, recovery ethanol and to be concentrated into relative density be 1.30 45-55 ℃ of drying, makes dry extract 1;
(2), the decocting with medicinal 10 times of amounts and 8 times of amounts in the Six-elements such as Caulis Sinomenii, Radix Salviae Miltiorrhizae residue and Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Dioscorea septemloba Thunb. boils twice, each 2 hours, merge and consider liquid, being evaporated to relative density is 1.30,45-55 ℃ of drying, make dry extract 2;
(3), two kinds of dry extracts of (1) and (2) preparation gained are pulverized, fully mix homogeneously adds ethanol and makes adhesive, adds appropriate amount of starch and makes filler, makes the 1000g granule.
Claims (10)
1, a kind of medicine for the treatment of gout is characterized in that, it mainly is to be made by following raw materials in weight portion medicine:
Caulis Sinomenii 331-993 part Rhizoma Atractylodis (parched) 165-497 part Cortex Phellodendri 110-330 part,
Herba Leonuri 165-497 part Rhizoma Polygoni Cuspidati 165-497 part Dioscorea septemloba Thunb. 165-497 part.
2, the medicine of treatment gout according to claim 1 is characterized in that, wherein the amount of crude drug is:
Caulis Sinomenii 497-828 part Rhizoma Atractylodis (parched) 248-414 part Cortex Phellodendri 165-275 part,
Herba Leonuri 248-414 part Rhizoma Polygoni Cuspidati 248-414 part Dioscorea septemloba Thunb. 248-414 part.
3, the medicine of treatment gout according to claim 1 and 2 is characterized in that, wherein the amount of crude drug is:
220 parts of 331 portions of Cortex Phellodendris of 662 parts of Rhizoma Atractylodis (parched) of Caulis Sinomenii,
331 parts of 331 parts of Dioscorea septemloba Thunb. of 331 parts of Rhizoma Polygoni Cuspidati of Herba Leonuri.
4, the preparation method of claim 1,2 or 3 described medicines, it comprises the following steps:
(a) it is standby to take by weighing each crude drug Caulis Sinomenii, Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati and Dioscorea septemloba Thunb. ;
(b) Caulis Sinomenii of described weight proportion is carried out reflux, extract, twice with the ethanol of 70%-95%, merges extracted twice liquid, reclaim ethanol, 45-55 ℃ dry down, dry extract 1;
(c) Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati and the Dioscorea septemloba Thunb. of described weight proportion and the residue after the Caulis Sinomenii alcohol extraction are decocted with water twice, add water serves as easy not have powder at every turn, merge decocting liquid twice, filter to such an extent that consider liquid 2, concentrate considering liquid, to become proportion be 1.25～1.35 extractum, dry under 45-55 ℃, get dry extract 2;
(d) merge dry extract 1 and 2, it is ground into powder, be prepared into the active component of medicine of the present invention;
(e) the gained active component is mixed with adjuvant, make any medicament on the pharmaceutics.
5, medicine according to claim 1, wherein crude drug also has, Radix Salviae Miltiorrhizae 132-398 part, Fructus Aurantii Immaturus (parched) 132-398 part, Spina Gleditsiae 132-398 part.
6, medicine according to claim 2, wherein crude drug also has, Radix Salviae Miltiorrhizae 198-331 part, Fructus Aurantii Immaturus (parched) 198-331 part, Spina Gleditsiae 198-331 part.
7, medicine according to claim 3, wherein crude drug also has, 265 parts of Radix Salviae Miltiorrhizaes, 265 parts of Fructus Aurantii Immaturus (parched), 265 parts of Spina Gleditsiaes.
8, the preparation method of claim 5,6 or 7 described medicines, it comprises the following steps:
(a) it is standby to take by weighing each crude drug Caulis Sinomenii, Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Spina Gleditsiae, Radix Salviae Miltiorrhizae, Fructus Aurantii Immaturus (parched) and Dioscorea septemloba Thunb. ;
(b) Caulis Sinomenii, the Radix Salviae Miltiorrhizae with described weight proportion carries out reflux, extract, twice with the ethanol of 70%-95%, merges extracted twice liquid, reclaims ethanol, and be dry under 45-55 ℃, gets dry extract 1;
(c) Rhizoma Atractylodis (parched), Cortex Phellodendri, Herba Leonuri, Rhizoma Polygoni Cuspidati, Spina Gleditsiae, Fructus Aurantii Immaturus (parched), Dioscorea septemloba Thunb. and the Caulis Sinomenii of described weight proportion, the residue after the Radix Salviae Miltiorrhizae alcohol extraction are decocted with water twice, add water serves as easy not have powder at every turn, merge decocting liquid twice, filter to such an extent that consider liquid 2, concentrate considering liquid, to become proportion be 1.25～1.35 extractum, dry under 45-55 ℃, get dry extract 2;
(d) merge dry extract 1 and 2, it is ground into powder, preparation cost invention active constituents of medicine;
(e) the gained active component is mixed with adjuvant, make any preparation on the pharmaceutics.
9, according to claim 5 or 8 described methods, wherein in step (c), during extracting in water, add 10 times in water for the first time, decocted 2 hours, add 8 times in water the second time, decocted 1.5 hours.
According to claim 5,6,7 described medicines, it is characterized in that 10, this medicine is tablet, granule, powder, capsule, drop pill, intramuscular injection agent, the instillation on the pharmaceutics.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB03119592XA CN100367997C (en) | 2003-03-12 | 2003-03-12 | Medicine for treating arthrolithiasis and preparing method thereof |
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| CNB03119592XA CN100367997C (en) | 2003-03-12 | 2003-03-12 | Medicine for treating arthrolithiasis and preparing method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402065C (en) * | 2006-05-30 | 2008-07-16 | 陕西九州生物科技股份有限公司 | Capsule for treating gout and preparation process thereof |
| CN101164585B (en) * | 2007-06-21 | 2010-05-19 | 济南康众医药科技开发有限公司 | Medicine for curing gout and its preparing method |
| CN1899439B (en) * | 2006-06-30 | 2011-05-11 | 杨雄志 | Chinese medicine composition for treating goat and its use |
| CN103432279A (en) * | 2013-08-07 | 2013-12-11 | 许春桂 | Method for preparing soaking agent for preventing and treating gout with decoction dregs and product |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297309C (en) * | 2001-12-24 | 2007-01-31 | 重庆市北碚区本草生物研究所 | External medicine composition for curing arthralgia-syndrome of joint and preparation process thereof |
-
2003
- 2003-03-12 CN CNB03119592XA patent/CN100367997C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402065C (en) * | 2006-05-30 | 2008-07-16 | 陕西九州生物科技股份有限公司 | Capsule for treating gout and preparation process thereof |
| CN1899439B (en) * | 2006-06-30 | 2011-05-11 | 杨雄志 | Chinese medicine composition for treating goat and its use |
| CN101164585B (en) * | 2007-06-21 | 2010-05-19 | 济南康众医药科技开发有限公司 | Medicine for curing gout and its preparing method |
| CN103432279A (en) * | 2013-08-07 | 2013-12-11 | 许春桂 | Method for preparing soaking agent for preventing and treating gout with decoction dregs and product |
| CN103432279B (en) * | 2013-08-07 | 2015-05-27 | 许春桂 | Method for preparing soaking agent for preventing and treating gout with decoction dregs and product |
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| CN100367997C (en) | 2008-02-13 |
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