CN1528155A - Soybean isoflavone milk and milk product - Google Patents
Soybean isoflavone milk and milk product Download PDFInfo
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- CN1528155A CN1528155A CNA031359566A CN03135956A CN1528155A CN 1528155 A CN1528155 A CN 1528155A CN A031359566 A CNA031359566 A CN A031359566A CN 03135956 A CN03135956 A CN 03135956A CN 1528155 A CN1528155 A CN 1528155A
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Abstract
The present invention relates to a soybean isoflavone milk and milk product. It is made up by using milk and soybean isoflavone, mixing them uniformly and adopting ultramicroemulsification process. As compared with existent technology said product has higher nutrient components, it is suitable for female, specially is suitable for climacteric woman to drink. The constantly drinking can relieve menopausal syndrome, can prevent ostroporosis, reduce fat, improve hyperplasia of mammary glands and hysteromyoma and reduce disease incidence of mammary cancer and cardiac disease, etc.
Description
Technical field: the present invention is a kind of isoflavones milk and dairy produce, belongs to the technical field of food.
Technical background: the applicant once applied for to Patent Office of the People's Republic of China that a name was called the patent of " isoflavones milk powder and preparation method thereof ", and its application number is: 03117325X; It is the product that mixes, adopts the method for ultra micro emulsification to make milk powder, isoflavones and food additives; In actual development process, find: be processed into after obtaining milk milk powder, again produce on this basis contain isoflavones milk powder product with regard to more complicated, increased the production process process, strengthened product cost; Investigation is simultaneously found: the consumer is ready the drinking milk more, rather than milk powder, and along with the continuous development of economic construction, the source of milk has not been the factor of restriction product development simultaneously; Retrieval through patent documentation, we find to have and add isoflavones in the drinking water patent application, as: application number is 031111408 " processing method of isoflavones beverage ", " a kind of compound method of soybean isoflavone health beverage " of 03100802X; They are not mentioned and produce milk and the dairy produce that contains the isoflavones composition; So so far, this respect still is blank; In addition, we investigate discovery: many dairy produces are directly to make of milk, and such as ice cream, sour milk or the like, the consumer groups of these products are bigger, particularly in, elderly woman and the low or superfluous crowd of estrogen.
Summary of the invention: the objective of the invention is to: a kind of isoflavones milk and dairy produce are provided; To increase a kind of new food variety to the human body beneficial; Utilized this natural plants estrogen that has security of isoflavones, milk offers human body nutrition preferably again simultaneously.The present invention constitutes like this: calculate according to weight proportion, it is made by milk 98-99.95%, isoflavones 0.05-2%.Specifically: it is made by 98 kilograms of milk, 2 kilograms of methods with ultra micro emulsification of isoflavones, also can directly add isoflavones in milk, stir and make the milk that contains isoflavones.For dairy produce: the 99.75-99.5 kilogram of will suckling, isoflavones 0.25-0.5 kilogram mix, utilize existing technology can be made into condensed milk.Certainly, be made into sour milk with milk 99.75-99.5 kilogram, isoflavones 0.25-0.5 kilogram.Milk 99.75-99.5 kilogram, isoflavones 0.25-0.5 kilogram can also be made into ice cream after mixing, add other food additives.
Compared with prior art: the invention provides and relatively be suitable for human edible common food, the product that obtains has than higher nutrition, and it is wide to be suitable for the crowd; Particularly, the low or superfluous crowd of elderly woman and estrogen, the patient of male prostate illness, often drink and to alleviate climacteric metancholia, prevention of osteoporosis, reduce fat, improve the proliferation of mammary gland with fibroid, reduction breast cancer, the cardiopathic incidence of disease, body inner estrogen level is adjusted to poised state; Isoflavones can help the utilization of people to calcium in the milk; Compare with the milk powder that contains isoflavones of the applicant's application, this process of producing product is simple, nutrition can not lost, be had more to nutrient, it is fresh, good to eat simultaneously, low production cost, it is a kind of new milk system food kind, since wide in variety, so have reasonable market sale prospect.
Embodiments of the invention 1: it makes the fresh milk that contains isoflavones by 98 kilograms of fresh milks, 2 kilograms of methods with ultra micro emulsification of isoflavones.
Embodiments of the invention 2: it is made for 0.05 kilogram by 99.95 kilograms of milk, isoflavones.Specifically: in milk, directly add isoflavones, stir and make the milk that contains isoflavones.
Embodiments of the invention 3: mix, utilize existing technology can be made into condensed milk for 0.25 kilogram 99.75 kilograms of fresh milks, isoflavones.
Embodiments of the invention 4: will suckle 0.5 kilogram of 99.5 kilogram, isoflavones mix, by fermentation, sterilization, sterilization can be made into sour milk.
Embodiments of the invention 5: be made into ice cream after 0.25 kilogram of 99.75 kilograms of milk, isoflavones mixed, adds other food additives.
Embodiments of the invention 6: disregard concrete ratio by milk with the isoflavones that from soybean, extracts and mix, satisfy crowd's taste or demand and get final product.
In order to prove this product for the human body beneficial, the applicant asks relevant department to do the experiment of " isoflavones milk is to the influence of removal ovary children mouse developing womb ", and concrete condition is:
Method: excise 14 age in days mouse bilateral ovaries, respectively with isoflavones milk 16g/kgwt (isoflavones milk high dose group), 8g/kgwt (dosage group in the isoflavones milk), 4g/kgwt (isoflavones milk low dose group), isoflavones 0.064g/kgwt (isoflavones group), diethylstilbestrol 0.001g/kgwt (diethylstilbestrol group), diethylstilbestrol 0.001g adds soybean isoflavones milk 16g/kgwt (diethylstilbestrol isoflavones milk high dose group), diethylstilbestrol 0.001g adds soybean isoflavones milk 8g/kgwt (dosage group in the diethylstilbestrol isoflavones milk), diethylstilbestrol 0.001g adds soybean isoflavones milk 4g/kgwt (diethylstilbestrol isoflavones milk low dose group)
Milk 8g/kgwt (model group) irritates stomach, every day 2 times, continuous 8 days is contrast with the mouse stomach of the same age of the other part fat of excision bilateral ovaries 8g/kgwt (sham-operation group) animal of suckling, measured the uterus coefficient on the 9th day behind the filling stomach, the difference of uterus coefficient between relatively each is organized.The result: each is organized between the coefficient of uterus, the model group minimum, and each group all has highly significant difference (P＜0.01) by comparison; Isoflavones milk high dose group, diethylstilbestrol group and stilbestrol isoflavones are suckled high, medium and low dosage group all apparently higher than sham-operation group (P＜0.05, P＜0.01); Each group of high, medium and low dosage of suckling isoflavones strengthens with increase of dosage, be dosage correlation, wherein, high dose group is apparently higher than low dose group (P＜0.05), middle dosage group then is lower than high dose group and is higher than low dose group, but not statistically significant (P＜0.05); Each group of isoflavones milk all is lower than diethylstilbestrol group (P＜0.05, P＜0.01) simultaneously; Between each group of diethylstilbestrol isoflavones milk, then, also be dosage correlation with the increasing and reduce of dosage, wherein, high dose group is starkly lower than low dose group (P＜0.05), and middle dosage group then is higher than high dose group and is lower than low dose group, but not statistically significant; The dosage group has the trend that is higher than the isoflavones group in the isoflavones milk, but not statistically significant (P＜0.05).Conclusion: isoflavones is suckled when estrogen level is low in vivo, has the effect of complementing estrogen deficiency, then may have antiestrogenic effect when estrogen level is too high in vivo.Therefore, when body produced unfavorable variation because of estrogen level is too high or too low, it should be useful using isoflavones milk.
This research adds the influence of the milk of isoflavones to the growth of removal ovary Mouse Uterus, observing its estrogen-like action and estrogenic antagonist, whether can resist that estrogen level is lower than or too highly provide experimental basis the adverse effect that body caused for inquiring into it.
Materials and methods:
1, animal: Kunming mouse, the one-level animal used as test,, 14 ages in days, body weight: 14.25 ± 0.43g, the Guiyang Medical College Experimental Animal Center provides, the quality certification: SCXK (Guizhou Province) 2002-0001.
2, tried thing: isoflavones milk
3, reagent and instrument: diethylstilbestrol (the Chinese Shanghai Xinyi Pharmaceutical Factory, lot number: 000201 second), electronic analytical balance (Mei Teletuo benefit instrument (Shanghai) Co., Ltd.).
4, experimental technique [1]
Mouse is with yellow Jackets 50mg/kg ip anesthesia, removal ovary animal excision bilateral ovaries, and the sham-operation animal is the other part fat of spay and keep ovary only, sews up a wound.Postoperative 3d is divided into 9 groups at random with 117 removal ovary animals, add 13 mouse of sham-operation group, totally 130 animals are divided into 10 groups: sham-operation group (milk 8g/kg), model group (milk 8g/kg), diethylstilbestrol group (diethylstilbestrol 0.001g/kg), isoflavones group (isoflavones 0.064g/kg), isoflavones milk high dose group
(isoflavones 0.128g/kg, add milk 15.832g/kg), dosage group (isoflavones 0.064g/kg in the isoflavones milk, add milk 7.936g/kg), isoflavones milk low dose group (isoflavones 0.032g/kg, add milk 3.968g/kg), diethylstilbestrol isoflavones milk high dose group (diethylstilbestrol 0.001g/kg adds soybean isoflavones 0.128g/kg and adds milk 15.831g/kg), dosage group in the diethylstilbestrol isoflavones milk (diethylstilbestrol 0.001g/kg adds soybean isoflavones 0.064g/kg and adds milk 7.935g/kg), diethylstilbestrol isoflavones milk low dose group (diethylstilbestrol 0.001g/kg adds soybean isoflavones 0.032g/kg and adds milk 3.967g/kg), grouping each group next day is pressed medicine and dosage ig separately, twice of every day, continuous 8d, 9d takes off cervical vertebra and puts to death animal, cut open and get the uterus, in time weigh, be calculated as follows the uterus coefficient, relatively the difference of uterus coefficient between each group.
5, t check between statistical method employing group.
The result
See Table 1.Each is organized between the coefficient of uterus, the model group minimum, and each group all has highly significant difference (P＜0.01) by comparison; Isoflavones milk high dose group, diethylstilbestrol group and stilbestrol isoflavones are suckled high, medium and low dosage group all apparently higher than sham-operation group (P＜0.05, P＜0.01); Each group of high, medium and low dosage of suckling isoflavones strengthens with increase of dosage, be dosage correlation, wherein, high dose group is apparently higher than low dose group (P＜0.05), middle dosage group then is lower than high dose group and is higher than low dose group, but not statistically significant (P＜0.05); Each group of isoflavones milk all is lower than diethylstilbestrol group (P＜0.05, P＜0.01) simultaneously; Between each group of diethylstilbestrol isoflavones milk, then, also be dosage correlation with the increasing and reduce of dosage, wherein, high dose group is starkly lower than low dose group (P＜0.05), and middle dosage group then is higher than high dose group and is lower than low dose group, but not statistically significant; The dosage group has the trend that is higher than the isoflavones group in the isoflavones milk, but not statistically significant (P＜0.05).
Table 1 isoflavones milk is to the influence of removal ovary children mouse developing womb (X ± S)
Group dosage (g/kg) n uterus coefficient (mg/100g)
Sham-operation group-13 88.0 ± 31.9
Model group-13 31.0 ± 10.3
Diethylstilbestrol 0.001 13 214.5 ± 46.8
*
Isoflavones 0.064 13 69.5 ± 33.0
Isoflavones suckles 16 12 144.3 ± 87.2
* △
8????????????????12?????????89.2±22.3
△△■□
4????????????????13?????????70.8±20.9
△△■■
The diethylstilbestrol isoflavones suckles 16 13 211.3 ± 54.8
* ▲
8????????????????12?????????223.8±91.4
**
4????????????????12?????????259.6±34.4
**△
Annotate: compare with each group
※ ※(P＜0.01); Compare * (P＜0.05) with the sham-operation group,
*Compare with the diethylstilbestrol group (P＜0.01)
△(P＜0.05),
△ △(P＜0.01); Compare with isoflavones milk high dose group
■(P＜0.05),
■ ■(P＜0.01); Compare with isoflavones milk low dose group
(P＜0.05); Compare with diethylstilbestrol isoflavones milk low dose group
▲(P＜0.05).
Discuss
It is 25-30d for the lactation of mouse, and be 35-55d the sexual maturing period of female mice
[2], 14 age in days mouse still are suckling mouse.Behind the excision suckling mouse ovary, the growth in its uterus promptly loses estrogenic support and can not continue normal growth.The molecular structure of isoflavones is similar to estrogen, has weak estrogen-like action
[3]Therefore the uterus that gives the removal ovary mouse of isoflavones and isoflavones milk can be grown, and has dosage correlation.The uterus coefficient of isoflavones milk high dose group is apparently higher than the sham-operation group, the sham-operation treated animal still is young mouse (26 age in days) in the time of may be with off-test, its ovary is all still immature relevant with developing womb, also show simultaneously, when the consumption of the isoflavones of pharmacology dosage milk greatly to a certain degree the time, it intends estrogen action also is stronger, in addition, the suckle uterus coefficient of each dosage group of isoflavones all is lower than diethylstilbestrol group (P＜0.05, P＜0.01), the stilbestrol of its effect well below pharmacology dosage is described, is weak estrogen receptor agonist.Though there was no significant difference between dosage group and the isoflavones group in the isoflavones milk, its trend is conspicuous,
As increase its significant difference of sample size and should be able to show, the amount of these two groups of contained isoflavones equates, thereby occur this kind trend whether with milk in lipid material promoted the absorption of isoflavones or flora, enzyme system that milk has changed mouse intestinal to a certain extent to improve the bioavilability of isoflavones, or be due to other the factor, worthly further probe into.Relatively the uterus coefficient between three dosage groups of diethylstilbestrol isoflavones milk is found, middle dosage group has the tendency that is lower than low dose group, but not statistically significant, high dose group then is starkly lower than low dose group (P＜0.05), illustrates that the isoflavones milk of doses has antiestrogenic effect.The existing weak estrogen-like action of this kind has the character of certain estrogenic antagonist again, meets the feature of partial agonist.
Conclusion
As partial agonist, isoflavones is suckled when estrogen level is low in vivo, has the effect of complementing estrogen deficiency, then may have antiestrogenic effect when estrogen level is too high in vivo.Therefore, when body produced unfavorable variation because of estrogen level is too high or too low, it should be useful using isoflavones milk.Can think isoflavones milk a kind of health food that is of value to WomanHealth of can yet be regarded as.
Claims (5)
1, a kind of isoflavones milk and dairy produce is characterized in that: calculate according to weight proportion, it is made by milk 98-99.95%, isoflavones 0.05-2%.
2, according to described isoflavones milk of claim 1 and dairy produce, it is characterized in that: it is made by 98 kilograms of milk, 2 kilograms of methods with ultra micro emulsification of isoflavones, also can directly add isoflavones in milk, stir and make.
3, according to claim 1,2 described isoflavones milk and dairy produces, it is characterized in that: the 99.75-99.5 kilogram of will suckling, isoflavones 0.25-0.5 kilogram mix, are made into condensed milk.
4, according to claim 1,2 described isoflavones milk and dairy produces, it is characterized in that: be made into sour milk with milk 99.75-99.5 kilogram, isoflavones 0.25-0.5 kilogram.
5, according to claim 1,2 described isoflavones milk and dairy produces, it is characterized in that: milk 99.75-99.5 kilogram, isoflavones 0.25-0.5 kilogram are made into ice cream after mixing, add other food additives.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA031359566A CN1528155A (en) | 2003-09-26 | 2003-09-26 | Soybean isoflavone milk and milk product |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA031359566A CN1528155A (en) | 2003-09-26 | 2003-09-26 | Soybean isoflavone milk and milk product |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181414A (en) * | 2011-12-27 | 2013-07-03 | 内蒙古伊利实业集团股份有限公司 | Anti-ageing yoghourt containing flavonoid compounds and production method for same |
| CN103181413A (en) * | 2011-12-27 | 2013-07-03 | 内蒙古伊利实业集团股份有限公司 | Anti-ageing lactobacillus beverage containing flavonoid compounds and production method for same |
| CN104479944A (en) * | 2014-11-17 | 2015-04-01 | 张仁刘 | Appearance-beautifying grape wine and preparation method thereof |
| CN114766549A (en) * | 2022-05-13 | 2022-07-22 | 江苏省农业科学院 | Non-thermal sterilization method for bovine colostrum |
-
2003
- 2003-09-26 CN CNA031359566A patent/CN1528155A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181414A (en) * | 2011-12-27 | 2013-07-03 | 内蒙古伊利实业集团股份有限公司 | Anti-ageing yoghourt containing flavonoid compounds and production method for same |
| CN103181413A (en) * | 2011-12-27 | 2013-07-03 | 内蒙古伊利实业集团股份有限公司 | Anti-ageing lactobacillus beverage containing flavonoid compounds and production method for same |
| CN104479944A (en) * | 2014-11-17 | 2015-04-01 | 张仁刘 | Appearance-beautifying grape wine and preparation method thereof |
| CN114766549A (en) * | 2022-05-13 | 2022-07-22 | 江苏省农业科学院 | Non-thermal sterilization method for bovine colostrum |
| CN114766549B (en) * | 2022-05-13 | 2023-12-29 | 江苏省农业科学院 | Bovine coloctrum non-thermal sterilization method |
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