CN1515892A - Microanalysis chip for detecting photometric absorption and its application method - Google Patents
Microanalysis chip for detecting photometric absorption and its application method Download PDFInfo
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Abstract
The present invention relates to a microanalysis chip for absorption photometric detection and its application method. It is made by using conventional microanalysis chip channel and coupling it with liquid core waveguide tube. It can implement high-sensitivity absorption photometric detection in microanalysis chip system.
Description
Technical field
The field that the present invention relates to is the micro-fluidic chip analysis, particularly relates to a kind of microanalysis chip and using method thereof that absorbance detects of carrying out.
Background technology
The micro-fluidic chip analysis is based on analytical chemistry and analytical biochemistry, with micro electronmechanical process technology is support, with the microchannel network is architectural feature, the collection of sample, pre-service, separation, reaction, detection etc. are partially integrated in several square centimeters the area, thereby finish separation, analysis and the detection of sample efficiently, apace.Since the early 1990s proposed micro-total analysis system, micro-fluidic chip analysis (Microfluidic Analysis) was in most active frontier development always, was representing the 21 century analytical instrument move towards microminiaturization, integrated developing direction.Usually, micro-fluidic chip is made of two-layer or multilayer chiop up and down, and the material of chip is monocrystalline silicon or quartzy or glass or high molecular polymer etc.Area of chip is about several square centimeters, and the microchannel width and the degree of depth are micron order, is generally closed microchannel network, by the hole turnover sample that links to each other with the microchannel perpendicular to chip.
The field that the present invention relates to is the research of the detection system on the relevant micro-fluidic chip.Detection system is the important component part of an analytic system.Micro-fluidic chip is little because of its chip volume, and sample size only is skin, the upgrading of receiving, and the reaction channel of detection place is generally tens microns wide, and therefore the requirement to its detection means and device has its singularity.
Absorption spectroanalysis method (also claiming the absorbance detection method) is a kind of applicability detection method widely, occupies critical role in analytical chemistry, also is one of detection method that is used for the earliest micro-analysis system.The wavelength coverage of photometric detection is about 185～1100 nanometers.But because the detection volume in the micro-fluidic chip passage absorption cell is little, it is short to absorb light path, causes adopting the absorption photometry detection sensitivity than low 2-4 the order of magnitude of conventional macrosystem, thereby the application in the micro-fluidic chip analytic system is very limited at present.In recent years there are a lot of researchs to be devoted to increase the absorption light path of photometric detection system on the micro-fluidic chip, to improve photometric detection sensitivity.
The technology of having delivered at present can be divided into following two classes: the one, and adopt micro-machined method to increase the light path of absorption cell, specifically comprise: the chip of making the high-aspect-ratio passage, directly detect perpendicular to passage, the degree of depth of passage is generally less than 100 microns, improve light path by this method, limited (the Laura Ceriotti of its effect, Jan Lichtenberg, et al., Micro Total Analysis Systems 2001,339-340) on chip, make U-shaped (Liang Z H, ChiemN, Ocvik G, Tong T, Fluri K, Harrison D J.Anal.Chem.1996,68:1040) or the circulation absorption cell of Z-shaped structure, introduce light and detection along channel direction, can obtain longer light path, but this structure is difficult to make and harmonize, any deviation all can cause the loss of analytic signal and the decline of sensitivity, limited by the scattering effect of incident light, detecting light path should not be greater than 150 microns (HS.Moosavi, Y.Jiang, et al., Electrophoresis, vol.21, pp.1291-1299,2000); The three-layer sandwich type chip is made the vertical channel absorption cell in the middle layer, it detects light path and reaches the millimeter level, but this chip intermediate vertical passage is made difficulty, sealing-in three-layer sandwich type chip is difficulty relatively, cost height (Jeffrey Wolk, MichaelSpaid, Morten Jensen, Richard MacReynolds, Knute Stevenson, and Ring-Ling Chien, Micro TotalAnalysis Systems 2001,367-368).Another kind of employing slab guide technology, top and bottom at chip make the light total reflection face, light is at a certain angle from the incident of entrance port, top, reflecting between the reflecting surface up and down, can repeatedly pass through passage, this method is very high to the accuracy requirement of incident optical window, needing very accurately, incident light could guarantee to detect signal in small detection window, the detection light path of report is 50-272 micron (Moosav H S, Jiang Y, Lester L, McKinnon G, Harrison D J.Electrophoresis, 2000,21:1291).Also have at the inner integrated mirror reflectance device of absorption cell, adopt making high reflectance minute surface or reflection channel (Verpoorte E, Manz A, Luedi H such as silicon MEMS etching, Bruno A E, Maystre F, Krattiger B, Widmer H M, Van der Schoot B H, De Rooij N F.Sens.Actuators.B.1992, B6:66) (Tiggelaar R M, Veenstra T T, Sanders R G P, Gardeniers J G E, Elwenspoek M C, Van den Berg A.Talanta.2002,56:331) (Hidekuni Takao, Toshihiko Noda, MitsuakiAshiki, Kazuhiro Miyamura, Kazuaki Sawada, Makoto Ishida, Micro Total Analysis Systems 2001,363-364), based on the multiple plane reflection configuration, the reflection light path is the millimeter level, because plane reflection is harsh to the light path requirement, the integrated reflector surface technology is had relatively high expectations in the chip absorption cell.
At present, the method for the various increase light paths of photometric detection does not all break through the inherent limitation that chip and passage microsize thereof are brought, thereby can't really break through the bottleneck of detectability, and its detection sensitivity can't reach conventional spectrophotometric level.And mostly need a little exigent little manufacturing process, and complex structure, the cost height has limited the application of photometry at chip field.
Liquid core waveguide pipe be a kind of be the light transmitting fiber of inner core with liquid, in liquid core waveguide pipe, light generation total reflection waveguide can be carried out almost harmless propagation.This technology has been used to the photometric detection field of macroscopic view.Existing studies show that uses liquid core waveguide pipe as absorption cell, and it detects absorbance and is proportional to concentration, meets langbobier law, and its distinguishing feature is to improve the range of linearity of the sensitivity and the measurement of spectrophotometric method greatly.But, there is not liquid core waveguide pipe to be applied to the report of photometric detection as yet in the microanalysis chip field.
Summary of the invention
The objective of the invention is to break through the inherent limitation that chip and passage microsize thereof are brought, a kind of microanalysis chip and using method thereof that absorbance detects of carrying out is provided, effective light path of photometric detection is improved 2-3 more than the order of magnitude, significantly improve the spectrophotomelric assay sensitivity on the chip.
The microanalysis chip that absorbance provided by the invention detects, it is a kind of microanalysis chip that long light path absorbance detects that is used for based on the liquid core waveguide principle, according to the present invention, the absorption cell of microanalysis chip is made of microanalysis chip passage and the coupling of external liquid core waveguide pipe.
According to the present invention, described liquid core waveguide pipe is to remain test fluid in a kind of pipe to be flow through, and utilizes the character of tube wall to realize light kapillary of transmitting of total reflection or reflection continuously in the pipe internal flow.The cross sectional configuration of liquid core waveguide pipe internal channel is circular or oval.The internal diameter of liquid core waveguide pipe internal channel is in 0.1 micron-5 millimeters scope, and pipe thickness is in 1 micron-1 cm range.The pipe range of liquid core waveguide pipe is in 5 millimeters-50 meters scopes.
According to the present invention, be to realize the waveguide of light in waveguide, among the inwall of employed waveguide, outer wall, tube wall three parts itself, have at least a part to have reflection or total reflection function to light.
According to the present invention, an employed class waveguide is the optical waveguide that utilizes the difference realization of refractive index, it is characterized in that, among the three of inwall, outer wall, tube wall of waveguide own, at least one adopts refractive index to be lower than the material of tube fluid.
According to the present invention, employed another kind of waveguide is to utilize the principle of light reflection to realize optical waveguide, it is characterized in that among the inwall of described waveguide, outer wall, the three of tube wall own, at least one employing has the material of efficiency light reflecting properties.
According to another characteristics of the present invention, microanalysis chip internal channel and liquid core waveguide tube passage coupling interface adopt the interface of little dead volume, help reducing the dispersion of sample band at interface, simultaneously, be difficult for retaining bubble at interface, in order to avoid disturb normal measurement operation.
The microanalysis chip using method that absorbance of the present invention detects, during practical measurement, the incident ray of detection system light emitted enters liquid core waveguide pipe by the waveguide inlet, by tube fluid, is derived by the waveguide outlet, is detected by photodetector.The light that light source sends is by absorption of fluids in the liquid core waveguide pipe, thus the variation of generation absorbance.Advantageously, the incident light of light source and waveguide center of circle coaxial alignment to improve the incident light energy, promptly improve the signal to noise ratio (S/N ratio) of measuring.By the light that waveguide outlet is derived, adopt with the coaxillay aligned optical fiber of waveguide and collect, lead-in light detecting device again is to improve the efficient of collecting light.
According to using method of the present invention, adopt long waveguide to help improving effective light path of light absorption.
According to using method of the present invention, the waveguide inner fluid utilizes the pipe outer wall to realize waveguide relatively, loses effective light path of photometric detection because of there being the transmission of light in tube wall; For the waveguide of equal length, utilize inside pipe wall to realize that waveguide Billy realizes that with the pipe outer wall waveguide has longer effective light path.
According to using method of the present invention, when utilizing the pipe outer wall to realize waveguide, adopt thin liquid core waveguide tube wall, help improving effective light path of absorption cell.
According to using method of the present invention, for adopting light transmissive material to make the waveguide of tube wall, adopt light inlet port outer wall at waveguide, cover lighttight light screening material, minimizing enters without the useless light of fluid, can significantly improve the sensitivity of photometric detection.Advantageously, all cover lighttight light screening material, can further improve the sensitivity of photometric detection at the coupled end outer wall and the end face of liquid core waveguide pipe and chip.
Major advantage of the present invention is: can carry out highly sensitive photometric detection on the microanalysis chip of small size; Required volume of sample is when being lower than the order of magnitude of 1 microlitre, and detection sensitivity can reach and surpass conventional ultraviolet-visible pectrophotometer fully; In addition, the length of waveguide is not subjected to the restriction of die size, and the liquid core waveguide pipe absorption cell is centimetre to decimeter grade, even meter level, reaches very high detection sensitivity; When liquid core waveguide pipe was very long, method that can employing dish curl reduced system bulk.The loss of liquid core waveguide pipe light is minimum, and antijamming capability is strong; Detection system is simple in structure, processing easily, and stable and reliable for performance, volume is little, is easy to integrated.
Another outstanding advantage of the present invention is that absorption cell is fairly simple to light source requirements, can make light source with miniature laser or light emitting diode, and without any need for the other light sources calibration device, can obtain stronger light detecting signal; In order to improve the integration of system, can use photodiode to make light detecting device, to reach whole microminiaturized requirement.
Another advantage of the present invention is to realize that various product continuous sample introduction detects.Test sample is promptly discharged the chip system by liquid core waveguide pipe, therefore can continuous sample introduction, can be used for online monitoring.
The present invention can be widely used in based on the continuous flow of chip and Flow Injection Analysis.
Description of drawings
Fig. 1. be the microanalysis chip that long light path absorbance detects that is used in accordance with a preferred embodiment of the present invention based on the liquid core waveguide principle.
Fig. 2. be the partial enlarged drawing of liquid core waveguide pipe and micro-fluidic chip coupling interface on Fig. 1 chip.
Fig. 3. be a example according to used liquid core waveguide pipe in the preferred embodiment of the present invention.
Fig. 4. be the xsect printing opacity figure of used liquid core waveguide pipe among Fig. 3 embodiment, contrasted and do not carried out shading treatment and the different effect of having carried out shading treatment.
Fig. 5. be according to portion of liquid core waveguide pipe input end office (EO) enlarged drawing in the preferred embodiment of the present invention absorption cell.
Fig. 6. demonstration is equipped with the outcome record figure of the microchip analysis system of Fig. 3 embodiment device to variable concentrations iron (II)-phenanthroline complex detection.
Fig. 7. show typical curve that microchip analysis system that Fig. 3 embodiment device is installed detects variable concentrations iron (II)-phenanthroline complex and at the canonical plotting of spectrophotometer replicate determination.
Fig. 8. be another example according to used liquid core waveguide pipe in the preferred embodiment of the present invention.
Fig. 9. demonstration is equipped with the outcome record figure of the full Teflon AF liquid core waveguide pipe microchip analysis system of Fig. 8 example structure to variable concentrations iron (II)-phenanthroline complex detection.
Figure 10. demonstration is equipped with the outcome record figure of the interior silver-plated liquid core waveguide pipe microchip analysis system of Fig. 8 example structure to iron (II)-phenanthroline complex detection.
Embodiment
With reference to accompanying drawing, below will describe according to a preferred embodiment of the present invention 1 in detail.
Fig. 1 is the micro-fluidic chip structural map of the coupling liquid core waveguide pipe that makes in accordance with a preferred embodiment of the present invention.Micro-fluidic chip is made up of last (1), following (2) two glass sheet, and glass sheet thickness is 1.7 millimeters.Adopt photomask and wet etching technique to process " worker " font passage last (1), microchannel (3) width is 100 microns, 30 microns of the degree of depth.With 1.7 millimeters diamond heads respectively in the end of " worker " font passage perpendicular to chip boring (4), (5).Adopt the method for classical high temperature bonding to realize going up (1), (2) two permanent involution down.Obtain the micro-fluidic chip that length, width are respectively 30 millimeters, 20 millimeters.Near 2.5 millimeters positions of transverse passage-way on " worker " font, with diamond glass cutter perpendicular to chip, be parallel to interconnection, cutting obtains "T"-shaped channel chip.Be parallel to the face fine sandpaper grinding and polishing of interconnection, obtain transparent plane (8), so that the light source direct irradiation.Adopt 0.35 millimeter method that drill bit is holed on chip, obtain the about 390 microns chip interface of diameter (6) at the exit passageway of T font passage.The waveguide that is connect (7) is 5.5 centimeter length.The import and export (4), (5) that internal diameter 0.5mm polyfluortetraethylene pipe are adhered to micro-fluidic chip are located, and chip is linked to each other with outside sample introduction equipment.
Fig. 2 is the partial enlarged drawing of liquid core waveguide pipe and micro-fluidic chip coupling interface.Adopt 375 microns of Teflon AF1600 liquid core waveguide pipe (7) external diameters, internal diameter is 50 microns.(ZEBRA Japan) extrudes droplet paint liquid at the glass surface of cleaning, with a termination pump line pump gas of liquid core waveguide pipe, prevents that center-aisle from advancing lacquer with the black paint pen; The other end vertically soaks in the paint droplet, picks up fast, and pump gas is till lacquer is done.Coat skim with the painting pen of doing at the waveguide sidewall and paint (11) uniformly, paint thick about 5～10 microns.The liquid core waveguide pipe outer end diameter that obtains is slightly less than 390 microns.With the careful insertion of Teflon liquid core waveguide pipe japanning end, liquid core waveguide tube end and interface bottom fluid-tight engagement.With epoxide-resin glue (14) set wave conduit.For integrated light-emitting diode light source on chip, as shown in Figure 2 near micro-fluidic chip passage and waveguide optical interface place with diamond saturating (1), (2) glass sheet, grind with file, with the fine sandpaper polishing, obtain a square box (15) and be used to embed the integrated light-emitting diode light source then.
Fig. 3 is a kind of outer quartzy liquid core waveguide pipe absorption cell light path synoptic diagram that covers Teflon AF film (9).The refractive index of Teflon AF film (9) is 1.29, and the refractive index of aqueous solution in the passage (10) is 1.3333, and quartz wall (8) refractive index is 1.51.The waveguide porch is incident angle greater than θ with the total reflection condition of waveguide
0=75.4 °.To propagating with the luminous energy total reflection in liquid core waveguide pipe less than 15.6 ° of incident angle incidents, when in the solution extinction material being arranged, light is absorbed, and produces absorbance and changes along channel axis.As can be seen from Figure 3, when with 15.6 ° of incident angle incidents, the light path by solution accounts for about 1/3rd of total optical path.With pure Teflon AF kapillary or inwall total reflection metal capillary, can make the length of light path more than or equal to waveguide; Adopt the very thin waveguide of quartz wall, in quartz wall, propagate the ratio of light path, also can improve light path to reduce light.
The waveguide that Fig. 4 has contrasted Fig. 3 is not carrying out shading treatment and the different effect of having carried out shading treatment.A1, A2, B1, B2 are the real figure of Teflon liquid core waveguide pipe test side CCD photomicrograph.Light source is the light emitting diode of 505 nanometers.A1, A2 are the light distribution plan that incident end face does not have painting.B1, B2 have carried out the light distribution plan of shading treatment shown in Figure 4 for the incident end.Wherein A1, B1 inject 0.2mM phenanthroline-iron (II) chromophoric solution, and A2, B2 inject blank phenanthroline solution.Can see that when not carrying out shading treatment, solution changes the variation contribution of light summation little, because major part is only by propagating in the quartz wall.After carrying out shading treatment, channel center changes more obvious.The proof shading treatment helps to reduce background, improves the detection sensitivity of system.
Fig. 5 is the design of liquid core waveguide pipe detection port.The liquid core waveguide pipe outlet is imported light into photomultiplier by optical fiber and is detected by the single-core fiber (17) of an external diameter greater than liquid core waveguide pipe.Detection port also is simultaneously to detect taphole, and we have designed a dilution liquid pool (16), keep the ambient stable of outlet detection place.Also can adopt additive method to detect, as adopting photodiode, aim at the liquid core waveguide pipe outlet and directly detect, simplified system realizes whole miniaturization so greatly.
Fig. 6 demonstration is equipped with the outcome record figure of the microchip analysis system of Fig. 3 embodiment device to variable concentrations iron (II)-phenanthroline complex detection.Adopt above Fig. 1 micro-fluidic chip coupling liquid core waveguide pipe system and device, the light emitting diode that reaches 505 nanometers is made light source, and photomultiplier and low-light-level measurement instrument detect phenanthroline-iron (II) and mix chromophoric solution as detecting device.Two passages of T shape channel chip are alternately injected Fe (II) standard serial solution (five solution of an order of magnitude) and blank solution respectively.
Fig. 7 shows typical curve that microchip analysis system that Fig. 3 embodiment device is installed detects variable concentrations iron (II)-phenanthroline complex and at the canonical plotting of spectrophotometer replicate determination.Sensing range 2.0～10 micromoles per liter standard solution.Curvilinear equation is y=0.0184x+0.0056, R2=0.9991; The curvilinear equation that parallel laboratory test records on spectrophotometer is y=0.011x-0.0028, R2=0.9994.Detect absorbance and surpass spectrophotometer, but do not reach the minimum of Theoretical Calculation.Detection sensitivity is subjected to light source monochromaticity simultaneously, the influence that the shading of light shield layer not exclusively waits.
Fig. 8 is a kind of absorption cell light path synoptic diagram that carries out liquid core waveguide by inner full-reflection.The total reflection tube wall can be the nontransparent material surface of low-refraction transparency material or high reflectance.
Fig. 9 is for adopting an embodiment of Fig. 8 structure, full TeflonAF liquid core waveguide pipe absorption cell, 6.3 centimetres of pipe ranges, the typical curve that iron (II)-phenanthroline complex is detected and at the canonical plotting of spectrophotometer replicate determination.Sensing range 2.0～10 micromoles per liter standard solution.Curvilinear equation is y=0.0514x+0.0135, R
2=0.9995, the curvilinear equation that parallel laboratory test records on spectrophotometer is y=0.0117x-0.0123, R
2=0.9992.
Figure 10 is for adopting another embodiment of Fig. 8 structure, and the absorption cell of interior silver-plated liquid core waveguide pipe is to the outcome record figure of iron (II)-phenanthroline complex detection.Adopt the 3cm absorption cell, its absorbance can reach spectrophotometric 3.7～4 times.
Claims (10)
1. the microanalysis chip that detects of an absorbance is characterized in that, the absorption cell of this chip is coupled by microanalysis chip passage and external liquid core waveguide pipe and constitutes.
2. the microanalysis chip that detects according to the described absorbance of claim 1, it is characterized in that: the liquid core waveguide pipe that adopts in the device, (a) be to remain test fluid in a kind of pipe to be flow through, utilize the character of tube wall to realize light kapillary of transmitting of total reflection or reflection continuously in the pipe internal flow; (b) cross sectional configuration of liquid core waveguide pipe internal channel is circular or oval; (c) internal diameter of liquid core waveguide pipe internal channel is in 0.1 micron-5 millimeters scope; (d) pipe thickness is in 1 micron-1 cm range; (e) pipe range of liquid core waveguide pipe is in 5 millimeters-50 meters scopes; (f) among waveguide inwall, outer wall, tube wall three parts itself, have at least a part to have reflection or total reflection function to light.
3. the microanalysis chip that detects according to claim 1 or 2 described absorbances, it is characterized in that, among the inwall of described waveguide, outer wall, the three of tube wall own, have at least one to adopt refractive index to be lower than the material of tube fluid or to have the material of efficiency light reflecting properties.
4. the microanalysis chip that detects according to claim 1 or 3 described absorbances is characterized in that, applies one deck refractive index at the inwall of described waveguide or outer wall and is lower than the coating of aqueous solution in the pipe or can carries out the coating material that high-level efficiency reflects to light.
5. the microanalysis chip that detects according to claim 1 or 2 described absorbances, it is characterized in that, adopt light transmissive material at described waveguide, light inlet port outer wall at waveguide covers lighttight light screening material, advantageously, coupled end outer wall and the end face at liquid core waveguide pipe and chip covers lighttight light screening material.
6. the microanalysis chip that detects according to the described absorbance of claim 1 is characterized in that, described microanalysis chip passage and the coupling of liquid core waveguide tube passage, and its joint portion sealing, advantageously, the interface of two passages has minimum dead volume.
7. the using method of the microanalysis chip that detects of the described absorbance of claim 1, it is characterized in that, the incident ray of detection system light emitted enters liquid core waveguide pipe by the waveguide inlet, the light that the absorption of fluids light source sends in the liquid core waveguide pipe, thereby the variation of absorbance takes place, derived by the waveguide outlet, photodetector detects.
8. the using method of the microanalysis chip that detects according to the described absorbance of claim 7, it is characterized in that, the incident light of light source and waveguide center of circle coaxial alignment, to improve the incident light energy, light by waveguide outlet derivation, after collecting with the coaxillay aligned optical fiber of waveguide, lead-in light detecting device again is to improve the efficient of collecting light.
9. the using method of the microanalysis chip that detects according to the described absorbance of claim 7 is characterized in that utilizing increases waveguide length, perhaps reduces the pipe thickness of utilizing the pipe outer wall to realize the waveguide of waveguide, improves effective light path of absorption cell.
10. the using method of the microanalysis chip that detects according to the described absorbance of claim 7, it is characterized in that, the dependence pipe outer wall of equal length and internal diameter is realized the waveguide of waveguide relatively, uses the waveguide that relies on inside pipe wall to realize waveguide, can obtain the effective light path of longer photometric detection.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102486456A (en) * | 2010-12-03 | 2012-06-06 | 鲍元进 | Liquid core waveguide detection device |
| CN102539361A (en) * | 2012-01-10 | 2012-07-04 | 浙江大学 | Long-path optical fiber-microfluidic chip sensor for detecting absorbance and refraction index |
| CN102954938A (en) * | 2011-08-29 | 2013-03-06 | 中国科学院电子学研究所 | Absorption luminosity detecting sensor based on micro-fluid control channel full-reflection integration light waveguide |
| CN103630638A (en) * | 2012-08-21 | 2014-03-12 | 株式会社岛津制作所 | Flow cell |
| CN105092492A (en) * | 2014-05-06 | 2015-11-25 | 黄辉 | Light guide capillary-based photometric analyzer and detection method thereof |
| CN105575842A (en) * | 2015-12-20 | 2016-05-11 | 合肥艾斯克光电科技有限责任公司 | Testing method for LED packaging adhesive |
| CN105642508A (en) * | 2015-12-20 | 2016-06-08 | 合肥艾斯克光电科技有限责任公司 | LED glue injecting and testing all-in-one machine |
| CN109001168A (en) * | 2018-03-27 | 2018-12-14 | 黄辉 | A kind of light-conducting capillaries photometer |
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2003
- 2003-01-03 CN CN 03114734 patent/CN1216281C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102486456A (en) * | 2010-12-03 | 2012-06-06 | 鲍元进 | Liquid core waveguide detection device |
| CN102954938A (en) * | 2011-08-29 | 2013-03-06 | 中国科学院电子学研究所 | Absorption luminosity detecting sensor based on micro-fluid control channel full-reflection integration light waveguide |
| CN102954938B (en) * | 2011-08-29 | 2014-08-27 | 中国科学院电子学研究所 | Absorption luminosity detecting sensor based on micro-fluid control channel full-reflection integration light waveguide |
| CN102539361A (en) * | 2012-01-10 | 2012-07-04 | 浙江大学 | Long-path optical fiber-microfluidic chip sensor for detecting absorbance and refraction index |
| CN102539361B (en) * | 2012-01-10 | 2014-07-23 | 浙江大学 | Long-path optical fiber-microfluidic chip sensor for detecting absorbance and refraction index |
| CN103630638A (en) * | 2012-08-21 | 2014-03-12 | 株式会社岛津制作所 | Flow cell |
| CN103630638B (en) * | 2012-08-21 | 2015-06-24 | 株式会社岛津制作所 | Flow cell |
| CN105092492A (en) * | 2014-05-06 | 2015-11-25 | 黄辉 | Light guide capillary-based photometric analyzer and detection method thereof |
| CN105575842A (en) * | 2015-12-20 | 2016-05-11 | 合肥艾斯克光电科技有限责任公司 | Testing method for LED packaging adhesive |
| CN105642508A (en) * | 2015-12-20 | 2016-06-08 | 合肥艾斯克光电科技有限责任公司 | LED glue injecting and testing all-in-one machine |
| CN109001168A (en) * | 2018-03-27 | 2018-12-14 | 黄辉 | A kind of light-conducting capillaries photometer |
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| CN1216281C (en) | 2005-08-24 |
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