CN1515544A - Method for high-yield extracting cystine, tyrosine and phenylalamine by hydrolyzing keratin - Google Patents
Method for high-yield extracting cystine, tyrosine and phenylalamine by hydrolyzing keratin Download PDFInfo
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- CN1515544A CN1515544A CNA031135013A CN03113501A CN1515544A CN 1515544 A CN1515544 A CN 1515544A CN A031135013 A CNA031135013 A CN A031135013A CN 03113501 A CN03113501 A CN 03113501A CN 1515544 A CN1515544 A CN 1515544A
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- tyrosine
- amino acid
- gelucystine
- phenylalanine
- hair
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Abstract
The method for extracting amino acid by utilizing separation of hydrolysed utilizes the keratin acidic hydrolytic raw liquor and adds alcohol-water mixed solution according to a certain ratio to make dilution, then in the course of making them flow through active carbon adsorption column it can implement complete separation of cystine, tyrosine and alanine, and can greatly reduce production cost. It uses animal keratin including pig hair, cattle hair, sheep hair, horsehair, chicken feather, duck feather and human hair as raw material, and obtain high yield of the amino acids.
Description
The present invention relates to the amino acid whose biochemical method of hydrolysis of keratin matter separation and Extraction, after particularly utilizing the acidic hydrolysis stoste of keratin protein to add alcohol-water mixture dilution in proportion, realize that in the process of its active carbon adsorption column of flowing through the thorough of Gelucystine, tyrosine and phenylalanine separates, thereby obtain the high recovery rate of product separation.Isolating amino acid product can be widely used as medical material, foodstuff additive.
The existing report of the amino acid whose processing method of protein hydrolysate separation and Extraction, prior art 1. resinbed is analysed amino acid system, and the total recovery rate of amino acid is 15.4%; 2. and 3. prior art is the amino acid system of adsorption column and ion-exchange chromatography, isolates seven seed amino acids respectively from pig Hydrolysis of dried blood powder liquid, and total recovery rate is 14.36% and six seed amino acids, and total recovery rate is 19.1%; Prior art is application for a patent for invention 85102242 4..This separation method is different from above four kinds of partition methods, the key distinction is specificity amino acid separation product from keratin protein acidic hydrolysis liquid, and method is that the active carbon adsorption column of flowing through again just can be realized the amino acid product separation, obtains high recovery rate after water diluted former hydrolyzed solution.
The objective of the invention is for reducing product cost, keratin protein with cheapness is a raw material, application is with the dilution method of the mutually mixed in proportion solution of the organic solvent of clear water and pure dissolubility to former hydrolyzed solution, realized the separating fully of Gelucystine and tyrosine reduced production cost significantly.The present invention is that raw material is isolated the amino acid product with animal keratin such as pig hair, wool, ox hair, horsehair, chicken feather, drake feather, people send out.The one, effectively improved the use value of above-mentioned keratin protein; The 2nd, reduced the pollution of waste to physical environment, have good social benefit and economic benefit.
The present invention compared with the prior art, adopted diverse separation method, mainly be to utilize pure water dilution method to reduce the acidity of acidic hydrolysis protein hydrolyzate, allow its active carbon adsorption column of flowing through then, tyrosine and phenylalanine are adsorbed on the activated carbon column fully, make in the mixed solutions such as Gelucystine of the adsorption column of flowing through to contain tyrosine hardly.For a long time, in traditional Gelucystine isolation technique, because Gelucystine and the solubleness of tyrosine in water are all less, if utilize the little principle of solubleness with them when protein hydrolyzate separates, they are but mixed in together mutually, not only have a strong impact on the quality of their single amino acid compositions, and have a strong impact on the separation recovery rate.The present invention has solved this technical barrier from separation process technique.
Embodiment
Send out and place 800 milliliters of round-bottomed flasks with 100 gram people, add 300 milliliters of concentrated hydrochloric acids, be warmed up to more than 110 ℃, kept constant temperature 10 hours.The mixed solution that adds 3000 milliliters of clear water and 300 milliliter of 95% alcohol then to hydrolyzed solution; stir; allow it flow through GH-15 granulated active carbon post is housed; tyrosine and phenylalanine are attracted to activated carbon column, and other amino acid such as Gelucystine are hardly by charcoal absorption, with basic solution this solution that neutralizes; the cystine knot partial crystallization goes out; obtain Gelucystine crude product 12 grams,, obtain Gelucystine elaboration 9.8 grams again through dissolving decolouring N-process.When flowing through activated carbon column with aqueous ethanolic solution, all separate tyrosine and phenylalanine, after redistillation goes out alcohol, obtain tyrosine crude product crystallization precipitate, isolate tyrosine, phenylalanine is stayed in the tyrosine mother liquor, thereby realizes the separation to them.Through the decolorizing and refining program, obtain tyrosine elaboration 1.4 grams respectively, phenylalanine elaboration 1.6 grams.
Reference:
1. from pig blood powder protein matter, replenish and separate multiple amino acids " amino acid magazine " first phase 45-47 page or leaf in 1984.
2. from pig Hydrolysis of dried blood powder liquid, separate the 47th page of seven seed amino acids " amino acid magazine " first phase in 1984 continuously with adsorption column and ion exchange column.
3. gac---the 48th page of technology " amino acid magazine " first phase in 1984 of multiple amino acids such as L-phenylalanine in the ion exchange resin associating chromatographic separation pig Hydrolysis of dried blood powder liquid.
4. Chinese invention patent application CN85102242.
Claims (5)
1, the method for a kind of acid system hydrolyzed animal keratin matter separation and Extraction Gelucystine, tyrosine and phenylalanine, after the amino acid mixing stoste that hydrolysis changes into is diluted 1 to 15 times with alcohol-water mixture, allow its active carbon adsorption column of flowing through, through neutralizing and freeing, high recovery rate is obtained Gelucystine, tyrosine and phenylalanine product.
2, activated carbon column according to claim 1 is characterized in that any granulated active carbon of strong adsorptive power such as GH-15.
3, separation method according to claim 1 is characterized in that with 1 to 15 times of alcohol-water mixture dilution hydrolysising original liquid.
4, separable programming according to claim 1 is characterized in that the diluent of neutralized stream warp obtains Gelucystine, frees absorption amino acid acquisition tyrosine and phenylalanine.
5, release agent according to claim 1 is that water-soluble alcohol water is mixed in proportion liquid and acid ethanol solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031135013A CN1515544A (en) | 2003-01-02 | 2003-01-02 | Method for high-yield extracting cystine, tyrosine and phenylalamine by hydrolyzing keratin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031135013A CN1515544A (en) | 2003-01-02 | 2003-01-02 | Method for high-yield extracting cystine, tyrosine and phenylalamine by hydrolyzing keratin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1515544A true CN1515544A (en) | 2004-07-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031135013A Pending CN1515544A (en) | 2003-01-02 | 2003-01-02 | Method for high-yield extracting cystine, tyrosine and phenylalamine by hydrolyzing keratin |
Country Status (1)
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| CN (1) | CN1515544A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182297B (en) * | 2007-12-07 | 2010-07-28 | 湖北新生源生物工程股份有限公司 | Method for further reclamation of L-tyrosine from effluent of L-cystine production |
| CN101148416B (en) * | 2007-11-01 | 2012-08-08 | 广州博采生物科技有限公司 | Method for separating 15 kinds of amino acid one-time from protein hydrolyzate |
| CN102863366A (en) * | 2012-09-19 | 2013-01-09 | 北京金波绿泰科技有限公司 | Method for separating cystine and tyrosine from mixture of cystine and tyrosine |
| CN103787939A (en) * | 2014-03-12 | 2014-05-14 | 广州博采生物科技有限公司 | Method and device of separating and extracting amino acid products from protein hydrolysates |
| CN104130150A (en) * | 2014-08-01 | 2014-11-05 | 湖北新生源生物工程股份有限公司 | Method for separation and extraction of L-phenylalanine by protein hydrolysate |
| CN108324663A (en) * | 2018-03-23 | 2018-07-27 | 杨巧 | A kind of white orchid silkworm chrysalis facial mask and preparation method thereof |
| CN108524410A (en) * | 2018-03-23 | 2018-09-14 | 杨巧 | A kind of Jasmine silkworm chrysalis facial mask and preparation method thereof |
-
2003
- 2003-01-02 CN CNA031135013A patent/CN1515544A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148416B (en) * | 2007-11-01 | 2012-08-08 | 广州博采生物科技有限公司 | Method for separating 15 kinds of amino acid one-time from protein hydrolyzate |
| CN101182297B (en) * | 2007-12-07 | 2010-07-28 | 湖北新生源生物工程股份有限公司 | Method for further reclamation of L-tyrosine from effluent of L-cystine production |
| CN102863366A (en) * | 2012-09-19 | 2013-01-09 | 北京金波绿泰科技有限公司 | Method for separating cystine and tyrosine from mixture of cystine and tyrosine |
| CN102863366B (en) * | 2012-09-19 | 2015-03-11 | 北京金波绿泰科技有限公司 | Method for separating cystine and tyrosine from mixture of cystine and tyrosine |
| CN103787939A (en) * | 2014-03-12 | 2014-05-14 | 广州博采生物科技有限公司 | Method and device of separating and extracting amino acid products from protein hydrolysates |
| CN103787939B (en) * | 2014-03-12 | 2016-03-30 | 广州博采生物科技有限公司 | A kind of method of separation and Extraction amino acid products from protein hydrolyzate and equipment thereof |
| CN104130150A (en) * | 2014-08-01 | 2014-11-05 | 湖北新生源生物工程股份有限公司 | Method for separation and extraction of L-phenylalanine by protein hydrolysate |
| CN104130150B (en) * | 2014-08-01 | 2016-01-20 | 湖北新生源生物工程股份有限公司 | By the method for protein hydrolyzate separation and Extraction L-Phe |
| CN108324663A (en) * | 2018-03-23 | 2018-07-27 | 杨巧 | A kind of white orchid silkworm chrysalis facial mask and preparation method thereof |
| CN108524410A (en) * | 2018-03-23 | 2018-09-14 | 杨巧 | A kind of Jasmine silkworm chrysalis facial mask and preparation method thereof |
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