CN1515287A - Chinese medicine preparation for curing cardio-cerebral vascular diseases - Google Patents

Abstract

The present invention relates to a Chinese medicine preparation for curing the diseases of coronary heart disease, angina pectoris and mycocardiac infarction with obvious therapeutic effect. The medicine has the functions of promoting blood circulation, removing stasis, removing obstruction in the channel and relieving pain, and is made up by using pure Chinese medicinal materials of tribulus fruit, ginseng stem and leaf, salvia root and borneol. Said invention also provides its preparation method.

Description

A kind of Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease

Technical field:

The present invention relates to a kind of medicine for the treatment of cardiovascular and cerebrovascular disease, a kind of Chinese medicine preparation for the treatment of angina pectoris and cerebral infarction particularly is provided, belong to Chinese medicine treatment cardiovascular and cerebrovascular diseases medicament technical field.

Background technology:

Angina pectoris is a kind of common cardiovascular and cerebrovascular disease of serious harm human body health.Belong to motherland's medical science " thoracic obstruction " " pained " disease categories such as " cardiopalmus ", disease such as its clinical manifestation is angina pectoris, uncomfortable in chest, cardiopalmus, breathe hard.The cerebral infarction that modern medicine is referred to as is equivalent to the sick category of motherland's medical science cerebral infarction.It is a lot of that the paathogenic factor ancient Chinese medicine doctor is discussed, and primary disease is many owing to be worried angryly, or comfortable wine has a liking for fertile food, or chamber is hindered, overwork etc., so that YIN-deficiency in the lower part, liver-yang is opened cruelly, the endogenous wind turn, QI and blood is contrary disorderly, folder expectorant folder fire, the horizontal passages through which vital energy circulates of scurrying, hoodwink thinking and take place suddenly to fall forward dusk, all diseases of hemiplegia.Above disease nitroglycerin commonly used etc. have certain curative effect for emergent medicine rescues, and are the link clinical symptoms, but cure the symptoms, not the disease.Chinese medicine was being obtained significant progress aspect the treatment primary disease in the last few years, become one of important means of cardiovascular and cerebrovascular disease Comprehensive Treatments such as coronary heart disease, used medicine biases toward blood stasis heart arteries and veins, the resistance of breast sun numbness more, and the modern Chinese medicine theoretical research shows that the basic pathogenesis of coronary heart disease is a deficiency in origin and excess in superficiality, blood stasis due to qi deficiency.Therefore, most medicines have significant limitation in this disease of treatment, have influenced treatment of diseases.

Summary of the invention:

The invention provides a kind of Chinese medicine preparation for the treatment of cardiovascular and cerebrovascular disease, have blood circulation promoting and blood stasis dispelling, removing obstruction in the collateral to relieve pain, evident in efficacy, the characteristics for the treatment of both the principal and the secondary aspects of a disease at the same time.

The present invention is according to the understanding of Chinese medicine to the cardiovascular and cerebrovascular vessel pathogenesis, and in conjunction with the research of QI and blood correlation theory, the basic pathogenesis of coronary heart disease is a deficiency in origin and excess in superficiality, blood stasis due to qi deficiency.At angina pectoris is in the majority with type of obstruction of heart-blood clinically, so start with, dispel in the blood stasis of blood and stagnate from blood circulation promoting and blood stasis dispelling, general rule not bitterly, to remove the heresy of mark reality.And notice " gas is handsome with blood ", " the capable then blood of gas is capable ", the deficiency of vital energy then blood walking retardation in children is slow, because of void causes this pathological characteristic of the stasis of blood, is used the product of QI invigorating, reaches blood circulation promoting and blood stasis dispelling, removing obstruction in the collateral to relieve pain, the purpose for the treatment of both the principal and the secondary aspects of a disease at the same time.Selected have an addendum motive, promoting blood circulation and stopping pain, and thin network is regulated the flow of vital energy, and nourishes heart and transfers the pure Chinese herbal medicine of blood, by the theory of Chinese medical science prescription, through the strictness screening, concocts respectively, combines in conjunction with modern advanced technologies method for making.Following consumption is weight portion, and basic recipe is as follows:

Fructus Tribuli 60-5, Stem and leaf of Radix Ginseng 2-30

Radix Salviae Miltiorrhizae 10-45, Borneolum Syntheticum 0.05-0.8.

Its preferred weight proportioning is:

Fructus Tribuli 30, Stem and leaf of Radix Ginseng 10

Radix Salviae Miltiorrhizae 15, Borneolum Syntheticum 0.1

Processing procedure of the present invention is as follows:

Get Radix Salviae Miltiorrhizae and all be ground into fine powder or partly beat powder, partly or entirely Radix Salviae Miltiorrhizae adds alcohol reflux, and concentration of alcohol is between the 10-90%; Stem and leaf of Radix Ginseng and Fructus Tribuli extracting in water extract 1-6 time, and concentrating under reduced pressure adds ethanol precipitation, concentration of alcohol 50%-90%; Filter, decompression filtrate recycling ethanol is not to there being the alcohol flavor, and behind the thin up, filtrate is handled the good macroporous adsorptive resins of balance earlier by giving, colourless with washed with de-ionized water to effluent, discard cleanout fluid, use the 50-90% ethanol elution, collect eluent, collect ethanol elution, concentrating under reduced pressure is concentrated into relative density 1.10 (50 ℃), spray drying; With above-mentioned Radix Salviae Miltiorrhizae medicated powder or Radix Salviae Miltiorrhizae extract and appropriate amount of starch mixing, the reuse cover grinds method and Borneolum Syntheticum mixing, granulates, and makes granule, promptly gets medicine of the present invention.

Above-mentioned medicament can be a said dosage form on any pharmaceutics.

[function with cure mainly] blood circulation promoting and blood stasis dispelling, removing obstruction in the collateral to relieve pain.Cure mainly stabbing pain over the chest, uncomfortable in chestly do not relax, palpitation and uneasiness, the dyspnea with shortness of breath hemiplegia, crooked mouth and tongue, stiff tongue and retardation in speech or is in silence felt to go down or disappears, has a dizzy spell, and dimly pale tongues etc. are with disease such as wind-phlegm stasis of blood resistance card grade.Be suitable for the angina pectoris stagnation of heart-blood disease and the cerebral infarction of obstruction of qi in the chest and cardialgia.

[usage and consumption] is oral, a 1-2 grain, 3 times on the one.

[specification] every dress 0.4g

[storage] is airtight, protection against the tide.

Angina pectoris that modern medicine is referred to as, belong to " thoracic obstruction ", " pained ", " cardiopalmus ", " precordial pain with cold limbs " category in motherland's medical science, motherland's medical science has the opinion of " gas is handsome with blood ", " the capable then blood of gas is capable ", gas with the blending of blood just like water and milk, all QI and blood are regulated then to seek and are defended harmony, and it sees the perverse then empty stasis of blood of QI and blood.Primary disease is more common in middle-aged and elderly people, and its cause of disease mainly is because stagnation of heart-blood, and depression of liver-QI is overworked, and loses and controls wrong treatment, can not agitate blood vessels, thrombosis, and the blood clots absorbing stagnates, the stasis of blood and obstructed, stagnation of QI and blood may bring about pain.Belong to deficiency in origin and excess in superficiality, blood stasis is this, and the deficiency of vital energy is mark.At thoracic obstruction disease clinically the card of stagnation of heart-blood, the suitable addendum motive of its Therapeutic Principle, promoting blood circulation and stopping pain are arranged more.Fructus Tribuli is a monarch drug in the side; Radix Salviae Miltiorrhizae is a ministerial drug; Stem and leaf of Radix Ginseng makes Borneolum Syntheticum for assistant, brings into play blood circulation promoting and blood stasis dispelling jointly, the merit of removing obstruction in the collateral to relieve pain.

Following pharmacological evaluation has confirmed that pharmaceutical preparation of the present invention has pharmacologically actives such as treatment angina pectoris disease treatment and analgesia:

The experiment medicine: the puncture ginseng capsule provides lot number by chemistry chamber, Jilin Prov. Inst. of Chinese Medicine and Chinese Medical Science: 20010101.

Positive control drug is an XINKESHU PIAN, produces lot number: 20010106 by the pharmaceutical factory of traditional Chinese medicine of Weifang, Shandong medicine Group Plc.

Laboratory animal: healthy adult hybrid dog, body weight 11-16kg, the male and female dual-purpose is provided by laboratory animal portion of Jilin University.Kunming mouse, the male and female dual-purpose.The Wistar rat, the male and female dual-purpose.Provide by Changchun High-technology Medical Animal Experiment Research Center.The animal quality quality certification number: 10-5113 10-5112.

Experimental apparatus: Japanese photoelectricity company produces RM-6000 type polygraph; Beijing Puli gives birth to LBY-self-cleaning rotary viscosimeter; The multi-functional platelet aggregation instrument of the Shanghai STA-4 of Kodak test instrunment factory type.

Experimental technique:

1. the puncture ginseng capsule is to the hemodynamic influence of anesthetized open-chest dog

30 of hybrid dogs are divided into 5 groups, 6 every group.Matched group waits the capacity normal saline, and the positive drug group is given XINKESHU PIAN 0.38g/kg, and experiment component is puncture ginseng capsule 5.10,2.55, three dosage groups of 1.28g crude drug/kg, all adopts duodenal administration.

With pentobarbital sodium 30mg/kg iv anesthesia, back of the body position is fixing, and skin of neck cuts, and tracheal intubation connects artificial respirator (WH-2 type, Tianjin medical apparatus and instruments factory produces), separates carotid artery, connects the AP-601 amplifier, measures blood pressure.Separate femoral artery, connect the AP-601 amplifier, carry out the ventricle interpolation pipe, measure left indoor pressure, end diastolic pressure, and through the maximum rate of change (± dp/dt max) of differential processor EQ-601G mensuration left indoor pressure.The 4th intercostal is executed thoracotomy in the left side, exposes heart, cuts off pericardium, does the pericardium art.Separate LCA and aortic root, place battery effusion meter probe (MFV-1100 type, Japanese photoelectricity company produces), measure coronary flow and aorta flow.Extremity connect limb lead, and bioassay standard II leads electrocardiogram, calculate heart rate.Separate duodenum, in order to administration.Above-mentioned each index synchronous recording is in polygraph (RM-6000 type, Japanese photoelectricity company produces).Art finishes, and stablizes 15 minutes, writes down before the administration and 5,10,20,30,45,60,90,120,150,180,240 minutes all indexs behind the medicine.Got tremulous pulse, venous blood in 30,60,120,180,240 minutes before administration and behind the medicine, measure oxygen content with blood oxygen instrument (Kang Ni-158, produced in USA).And, calculate two-level index: mean arterial pressure, cardiac index, SI, stroke work index, total peripheral resistance, coronary resistance, myocardial oxygen consumption, myocardial oxygen consumption index, myocardium oxygen uptake rate, myocardial flow etc. according to formula.Experimental data measured value and variation fraction values and matched group are relatively organized a t check analysis.

1.1 influence to heart rate, blood pressure

Each dosage group of puncture ginseng capsule is not obvious to the heart rate influence.See Table 1.

Each dosage group of puncture ginseng capsule is not obvious to the blood pressure influence.See Table 2.

1.2 influence to left indoor pressure, left chamber easypro end pressure and the work done of left chamber

Each dosage group of puncture ginseng capsule is not obvious to the left ventricular pressure influence.See Table 3.

Each dosage group of puncture ginseng capsule is pressed not have obviously to easypro end, left chamber and is acted on.See Table 4.

Each dosage group of puncture ginseng capsule does not have obvious effect to the work done of left chamber.See Table 13.

1.3 influence to maximum rate of change of left indoor pressure and total peripheral resistance

Each dosage group of puncture ginseng capsule does not all have obvious effect to the maximum rate of change of left indoor pressure.See Table 5, table 6.

The XINKESHU PIAN group changed percentage rate total peripheral resistance was acted on obviously (P＜0.05) at 90-120 minute, and other each groups do not have obvious effect.See Table 10.

1.4 influence to heart output, myocardial flow and coronary resistance

Each dosage group of puncture ginseng capsule is not obvious to the heart output influence.See Table 7.

The XINKESHU PIAN group changes percentage rate effect obvious (P＜0.05) in 45-90 minute to myocardial flow, and puncture ginseng capsule 1.28g crude drug/kg changes percentage rate and acted on obviously (P＜0.05) at 45-120 minute; Puncture ginseng capsule 2.55g crude drug/kg changes percentage rate effect obvious (P＜0.05) in 30-120 minute; Puncture ginseng capsule 5.10g crude drug/kg changes percentage rate effect obvious (P＜0.01) in 30-120 minute; Other each groups do not have obviously effect.See Table 8.

The XINKESHU PIAN group changes percentage rate effect obvious (P＜0.05) in 45-90 minute to coronary resistance; Measured value effect in 120 minutes is (P＜0.05) obviously; Puncture ginseng capsule 1.28g crude drug/kg changes percentage rate effect obvious (P＜0.05) in 90-120 minute; Puncture ginseng capsule 2.55g crude drug/kg changes percentage rate effect obvious (P＜0.05) in 45-120 minute; Effect in measured value 60-120 minute is (P＜0.05) obviously; Puncture ginseng capsule 5.10g crude drug/kg changes percentage rate effect obvious (P＜0.05) in 45-120 minute; Other each groups do not have obviously effect.See Table 9.

1.5 to cardiac index, SI, heart output influence

Each dosage group of puncture ginseng capsule is not obvious to the cardiac index influence.See Table 11.

Each dosage group of puncture ginseng capsule is not obvious to the SI influence.See Table 12.

Each dosage group of puncture ginseng capsule is not obvious to the heart output influence.See Table 14.

1.6 influence to myocardial oxygen consumption, myocardial oxygen consumption index and myocardium oxygen uptake rate

1.6.1 effect to myocardial oxygen consumption

The XINKESHU PIAN group changes percentage rate effect obvious (P＜0.05) in 30-90 minute to myocardial oxygen consumption; Measured value was effect obvious (P＜0.05) in 120 minutes; Puncture ginseng capsule 1.28g crude drug/kg changes percentage rate effect obvious (P＜0.05) in 30-45 minute; Puncture ginseng capsule 2.55g crude drug/kg changes percentage rate effect obvious (P＜0.05) in 30-45 minute; Puncture ginseng capsule 5.10g crude drug/kg changes percentage rate effect obvious (P＜0.01) in 30-90 minute; Measured value was effect obvious (P＜0.05) in 45 minutes; Other each groups do not have obviously effect.See Table 15.

1.6.2 to the exponential effect of myocardial oxygen consumption

Each dosage group of puncture ginseng capsule is not obvious to the influence of myocardial oxygen consumption index.See Table 16.

1.6.3 effect to myocardium oxygen uptake rate

The XINKESHU PIAN group changes percentage rate effect obvious (P＜0.01) in 30-90 minute to myocardium oxygen uptake rate; Measured value was effect obvious (P＜0.05) in 60-90 minute; Puncture ginseng capsule 1.28g crude drug/kg changes percentage rate effect obvious (P＜0.01) in 30-45 minute; Puncture ginseng capsule 2.55g crude drug/kg changes percentage rate effect obvious (P＜0.01) in 30-90 minute; Puncture ginseng capsule 5.10g crude drug/kg changes percentage rate effect obvious (P＜0.001) in 30-90 minute; Measured value is in obviously (P＜0.05) other each obviously effects of group nothing of effect in 45-90 minute.See Table 17.

Experimental result shows that the puncture ginseng capsule can obviously increase myocardial flow, reduces coronary resistance, reduce myocardial oxygen consumption and oxygen uptake rate.

2. the puncture ginseng capsule is to the influence of experimental myocardial infarction dog

Get 30 of hybrid dogs, be divided into 5 groups at random, 6 every group, anaesthetize with pentobarbital sodium 30mg/kgiv.Back of the body position is fixing, and skin of neck cuts, tracheal intubation, connect WM-2 type artificial respirator, separate 1/3 place under the left anterior descending coronary artery, threading causes myocardial infarction in order to ligation, with the wet cloth formula absorption method mapping EECG of multiple spot, 24 of mapping points divide normal district (control point), infraction marginal zone and infraction center, art finishes, and stablizes 15 minutes.Record each point EECG, simultaneously get blood from femoral vein, survey AST, CPK and LDH value, as being worth before the administration, behind the ligation coronary artery 15 minutes, through duodenal administration, matched group waits the capacity normal saline, and the positive drug matched group gives XINKESHU PIAN 0.38g/kg, puncture ginseng capsule 1.28g crude drug/kg, 2.55g crude drug/kg, three dosage groups of 5.10g crude drug/kg, the record administration after 5,10,20,30,45,60,90,120,150,180,210,240,300,360 minutes EECG, showing ∑-ST with ST section total mV numerical table that raises, is N-ST with the number that leads of ST section rising＞2mV.Write down after 360 minutes, get blood thought-read flesh three enzymes for the second time.Experiment finishes, and it is dirty to core, and claims to weigh whole-heartedly, cuts off trunk root and atrium along coronary sulcus, claims left ventricle heavy.Be cut into 5 with left ventricle is cross-section equably, place nitro tetrazole orchid (N-BT) dye liquor, dyeing is 15 minutes in 37 ℃ of constant water bath box.(36 points/cm2) are measured every myocardium bilateral infarct (the non-dyeing of N-BT district) and non-infarct (N-BT dye district) with the drop point method of quadrature, every cardiac muscle is weighed, calculate every myocardial area, the gross area of the ventricle gross area and infarct calculates infarct and ventricle and cardiac weight percentage ratio.

2.1 influence to EECG

2.1.1 to the influence of degree of ischemia (∑-ST)

The XINKESHU PIAN group acted on obviously (P＜0.05) to the degree of ischemia measured value at 45-180 minute; Puncture ginseng capsule 1.28g crude drug/kg was effect obvious (P＜0.05) in 60-180 minute; Puncture ginseng capsule 2.55g crude drug/kg was effect obvious (P＜0.05) in 45-180 minute; Puncture ginseng capsule 5.10g crude drug/kg was effect obvious (P＜0.01) in 30-180 minute; Other each groups do not have obviously effect.See Table 18

2.1.2 influence to ischemia scope (N-ST)

The XINKESHU PIAN group acted on obviously (P＜0.05) to ischemia scope measured value at 45-180 minute; Puncture ginseng capsule 1.28g crude drug/kg was effect obvious (P＜0.05) in 60-180 minute; Puncture ginseng capsule 2.55g crude drug/kg was effect obvious (P＜0.05) in 45-180 minute; Change percentage rate and acted on obviously (P＜0.05) at 30-180 minute at obvious (P＜0.05) the puncture ginseng capsule 5.10g crude drug/kg of effect in 30-45 minute; Other each groups do not have obviously effect.See Table 19.

2.2 influence to MIS

XINKESHU PIAN group, middle dosage group, heavy dose of group all can obviously reduce infraction/reach whole-heartedly infraction/left heart area (P＜0.05).Other each groups do not have obviously effect.See Table 20.

2.3 to myocardium three enzymes (AST, CPK, influence LDH)

The XINKESHU PIAN group, puncture ginseng capsule 1.28g crude drug/kg, 5.10g crude drug/Kg can obviously reduce the content (P＜0.01) of serum AST.Other each groups do not have obviously effect.See Table 21.

The XINKESHU PIAN group, puncture ginseng capsule 1.28g crude drug/kg, 5.10g crude drug/Kg can obviously reduce the content (P＜0.05) of serum CK.Other each groups do not have obviously effect.See Table 22.

Each dosage group of puncture ginseng capsule is not obvious to the Serum LDH influence.See Table 23.

Experimental result proves that the puncture ginseng capsule is through duodenal administration, and (∑-ST), reduce myocardial ischemia scope (N-ST) dwindles myocardial infarct size (MIS), reduces serum AST, the content of CPK obviously to reduce degree of myocardial ischemia.Alleviate myocardial cell injury.Thereby myocardial infarct size dwindles, and destructiveness reduces, and ischemic myocardium is had protective effect.

3. to the influence of clotting time of mice

Get 50 of mices, be divided into five groups at random, dosage sees Table 24, and every day 1 time, continuous irrigation stomach 15 days behind the last medicine 1 hour, adopts slide method to measure clotting time.The results are shown in Table 24.

The influence of table 24. pair clotting time of mice

Group and dosage number of animals clotting time

G crude drug/kg n (min; X ± SD)

Normal control group 10 0.80 ± 0.14

XINKESHU PIAN 1.40g/kg 10 1.03 ± 0.17 ^*

Puncture ginseng capsule 18.65g crude drug/kg 10 1.05 ± 0.16 ^*

Puncture ginseng capsule 9.33g crude drug/kg 10 1.00 ± 0.14 ^*

Puncture ginseng capsule 4.66g crude drug/kg 10 0.94 ± 0.13 ^*

Annotate: compare with matched group ^*P＜0.05 ^*P＜0.01 ^{* *}The as follows summary in P＜0.001

Experimental result proves: the puncture ginseng capsule can make clotting time of mice prolong, and is remarkable with normal control group comparing difference.

4. to the hemorheological influence of blood stasis rat model

Get the wistar rat, male and female half and half are divided into six groups at random, press the gastric infusion of dosage shown in the table 25, every day 1 time, continuous 15 days.Behind the last medicine 1 hour, except that the normal control group, all the other respectively organize rat skin lower injection epinephrine 0.08ml/100g, inject 1 time in 4 hours at interval, between the biphasic injection epinephrine (front and back each 2 hours at interval) again, whole rats were immersed in the frozen water 5 minutes, dispose the back fasting, inferior rat aorta blood sampling in morning, anticoagulant heparin, give birth to LBY-N6A from clear rotary viscosimeter with Beijing Puli, measure rat whole blood viscosity and plasma viscosity.

25. to the hemorheological influence of blood stasis rat model (X ± SD)

Group and dosage number of animals whole blood viscosity plasma viscosity

G crude drug/kg n 10 40 120

Normal control group 10 10.82 ± 2.68 ^*5.82 ± 1.94 ^{* *}4.34 ± 1.17 ^{* *}1.66 ± 0.46

Model group 10 15.97 ± 4.60 8.91 ± 1.50 6.28 ± 0.92 1.61 ± 0.25

XINKESHU PIAN 1.30 10 12.72 ± 2.65 7.66 ± 1.16 5.59 ± 0.75 1.54 ± 0.34

Puncture ginseng capsule 17.22 10 12.19 ± 2.17 ^*7.16 ± 0.94 ^*5.20 ± 0.60 ^*1.75 ± 0.31

Puncture ginseng capsule 8.61 10 13.43 ± 1.52 8.05 ± 0.81 5.82 ± 0.51 1.49 ± 0.24

Puncture ginseng capsule 4.30 10 14.59 ± 3.38 8.16 ± 1.30 5.80 ± 0.85 1.61 ± 0.39

The result shows that the puncture ginseng capsule can make blood stasis rat model whole blood viscosity reduce, and is remarkable with the model group comparing difference, and little to the plasma viscosity influence.

5. to the influence (turbidimetry) of rat platelet aggregation function

Get 50 of rats, be divided into five groups at random, press the gastric infusion of dosage shown in the table 26, every day 1 time, continuous 15 days, behind the last medicine 1 hour, with 3% pentobarbital sodium difference anesthetized rat (30mg/kg), through ventral aorta blood sampling, preparation PRP and PPP, putting the multi-functional platelet aggregation instrument of SPA-4 afterwards, is that derivant is measured platelet aggregation rate (5 minutes) with ADP.

The influence of table 26. pair rat platelet aggregation function

Group and dosage number of animals 5min platelet aggregation rate (ADP)

n (％；X±SD)

The normal control group---10 69.04 ± 16.10

XINKESHU PIAN 1.30g/kg 10 46.28 ± 10.32 ^*

Puncture ginseng capsule 17.22g crude drug/kg 10 47.23 ± 9.61 ^*

Puncture ginseng capsule 8.61g crude drug/kg 10 50.01 ± 9.59 ^*

Puncture ginseng capsule 4.30g crude drug/kg 10 57.16 ± 8.56

The result shows that the puncture ginseng capsule can make the inductive platelet aggregation of ADP be suppressed, and is remarkable with the matched group comparing difference.

Experiment conclusion:

Laboratory observation the puncture ginseng capsule to effects such as normal anesthetized open-chest dog hemodynamics and myocardial oxygen consumption.Experimental result shows that the puncture ginseng capsule can obviously increase myocardial flow, reduces coronary resistance, reduce myocardial oxygen consumption and oxygen uptake rate.These effects of puncture ginseng capsule, relevant with direct coronary artery dilating, make it myocardial flow and increase.Can reduce cardiac energy and oxygen consumption, oxygen uptake rate is reduced.The directly raising of reflection cardiac pumping function, the peripheral blood vessel expansion is relevant.Prompting puncture ginseng capsule can improve systema cariovasculare functional, regulates heart blood supply oxygen supply balance, and is to preventing and treating cardiovascular disease, significant as commonly encountered diseases such as coronary heart disease, myocardial ischemia.

Experiment adopts ligation anterior descending coronary (LAD) to cause acute myocardial infarction model.With epimyocardium electrograph mapping degree of myocardial ischemia and scope, the variation of sero-enzyme after quantitative tissue mensuration myocardial infarct size and the myocardial infarction.Experimental result proves that the puncture ginseng capsule is through duodenal administration, and (∑-ST), reduce myocardial ischemia scope (N-ST) dwindles myocardial infarct size (MIS), reduces serum AST, the content of CK obviously to reduce degree of myocardial ischemia.Alleviate myocardial cell injury.Thereby myocardial infarct size dwindles, and destructiveness reduces, and ischemic myocardium is had protective effect.Prompting puncture ginseng capsule has function of resisting myocardial ischemia.Simultaneously, the puncture ginseng capsule can prolong clotting time of mice, and blood stasis rat model whole blood viscosity is reduced, and the puncture ginseng capsule can make the inductive platelet aggregation of ADP be suppressed.Prompting puncture ginseng capsule has good function of promoting blood circulation to disperse blood clots.

Laboratory observation the puncture ginseng capsule to effects such as normal anesthetized open-chest dog hemodynamics and myocardial oxygen consumption.Experimental result shows that the puncture ginseng capsule can obviously increase myocardial flow, reduces coronary resistance, reduce myocardial oxygen consumption and oxygen uptake rate.These effects of puncture ginseng capsule are with the content of direct coronary artery dilating.Alleviate myocardial cell injury.Thereby myocardial infarct size dwindles, and destructiveness reduces, and ischemic myocardium is had protective effect.Prompting puncture ginseng capsule has function of resisting myocardial ischemia.Simultaneously, the puncture ginseng capsule can prolong clotting time of mice, and blood stasis rat model whole blood viscosity is reduced, and the puncture ginseng capsule can make the inductive platelet aggregation of ADP be suppressed.Prompting puncture ginseng capsule has good function of promoting blood circulation to disperse blood clots.

The specific embodiment:

Embodiment 1

Get Radix Salviae Miltiorrhizae 161g and be ground into fine powder, standby; Other gets Radix Salviae Miltiorrhizae 612g and adds 7 times of amount 90% ethanol, and reflux, extract, was once extracted 1 hour, filter, filtrate recycling ethanol is standby, and medicinal residues add 8 times of water gagings, decoct three times, filter, filtrate is concentrated into the clear paste that relative density is 1.20-1.30 (80 ℃), adding ethanol to determining alcohol is 70%, places, and filters, reclaim ethanol, merge with above-mentioned medicinal liquid, evaporate to dryness, standby; Get Stem and leaf of Radix Ginseng and Fructus Tribuli and add 10 times of water gagings, soaked 0.5 hour, decoct 2 times, each 1 hour, collecting decoction, being evaporated to relative density is 1.20-1.30 (80 ℃), add ethanol to determining alcohol and reach 70%, filter, decompression filtrate recycling ethanol is to there not being the alcohol flavor, behind the thin up, filter, filtrate is handled the good macroporous adsorptive resins of balance earlier by giving, and is colourless to effluent with washed with de-ionized water, discard cleanout fluid, use 70% ethanol elution, collect eluent, colourless to effluent, merge 70% ethanol elution, collect effluent, reclaim ethanol, concentrating under reduced pressure, be concentrated into relative density 1.10 (500C), spray drying; With above-mentioned medicated powder and Radix Salviae Miltiorrhizae medicated powder and appropriate amount of starch mixing, the reuse cover grinds method and Borneolum Syntheticum mixing, granulates, and makes 1000, promptly.

Table 1. puncture ginseng capsule to heart rate (inferior/min) influence (and X ± SD, n=6)

Administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----156.50 ± 22.528 158.17 ± 26.423 159.50 ± 28.550 157.50 ± 29.324 159.17 ± 28.923 160.33 ± 27.710

％ 0.84±2.457 1.58±3.533 0.29±2.292 1.42±5.528 2.27±5.332

XINKESHU PIAN 0.38g/kg 163.33 ± 24.468 158.67 ± 21.538 161.60 ± 21.976 161.33 ± 22.624 163.50 ± 23.467 163.17 ± 22.605

％ -2.55±5.334 -0.76±5.368 -0.92±6.390 0.40±7.060 0.35±8.337

Puncture ginseng capsule 1.28g crude drug/kg 149.33 ± 14.583 146.33 ± 16.281 146.67 ± 17.952 145.67 ± 18.715 148.00 ± 15.925 150.50 ± 14.937

％ -2.00±4.912 -1.85±5.725 -2.51±7.016 -0.86±4.967 0.96±6.737

Puncture ginseng capsule 2.55g crude drug/kg 150.67 ± 14.747 150.50 ± 16.682 150.00 ± 18.022 150.83 ± 19.793 151.67 ± 19.643 152.83 ± 21.821

％ -0.20±1.780 -0.56±3.898 -0.08±4.649 0.45±3.481 1.13±4.674

Puncture ginseng capsule 5.10g crude drug/kg 162.83 ± 12.529 165.50 ± 10.035 166.17 ± 9.538 166.17 ± 9.020 168.33 ± 6.563 166.33 ± 6.563

％ 1.75±2.260 2.19±2.916 2.22±3.555 3.63±4.029 2.28±2.549

After the administration (min)

60 90 120 150 180 240

161.33±27.580 161.17±26.955 163.17±25.514 163.67±25.828 162.33±26.051 161.83±26.769

2.89±4.484 2.88±5.345 4.24±4.540 4.55±4.878 3.68±5.211 3.36±6.432

163.50±24.395 161.83±24.211 160.83±25.111 161.00±25.108 160.50±25.470 160.67±25.288

0.45±8.650 -0.39±10.387 -1.04±10.837 -0.99±10.362 -1.29±10.742 -1.21±10.481

149.33±15.397 145.50±15.385 146.67±14.638 147.17±15.471 146.33±15.227 145.33±13.995

0.18±7.141 -2.25±9.151 -1.49±8.312 -1.15±8.969 -1.72±8.582 -2.35±8.362

155.83±20.351 153.17±21.619 151.33±19.765 152.17±22.131 151.50±21.989 150.33±22.462

3.31±6.066 1.46±6.442 0.32±5.469 0.78±6.876 0.30±6.601 -0.51±6.464

167.00±11.849 168.50±19.094 168.17±8.306 168.83±7.834 169.83±8.612 167.17±10.759

2.63±2.766 3.67±3.799 3.52±4.840 3.98±5.547 4.58±5.616 2.82±4.298

Table 2. puncture ginseng capsule to the influence of blood pressure (KPa) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----13.18 ± 1.060 13.23 ± 1.110 13.25 ± 1.421 13.31 ± 1.292 13.18 ± 1.258 13.18 ± 1.184

％ 0.43±2.349 0.45±5.596 0.98±4.950 0.04±5.453 0.00±4.711

XINKESHU PIAN 0.38g/kg 13.55 ± 1.194 13.11 ± 1.370 12.87 ± 12.00 12.97 ± 1.133 12.80 ± 1.260 13.06 ± 1.360

％ -3.36±3.172 -5.01±3.871 -4.27±3.311 -5.60±3.151 -3.69±3.313

Puncture ginseng capsule 1.28g crude drug/kg 12.34 ± 1.195 11.92 ± 1.861 11.85 ± 1.954 11.74 ± 2.102 11.88 ± 1.746 12.10 ± 0.874

％ -3.58±9.861 -4.28±9.995 -5.29±10.610 -4.02±7.388 -1.48±7.459

Puncture ginseng capsule 2.55g crude drug/kg 12.03 ± 1.019 11.90 ± 1.064 12.04 ± 0.992 12.10 ± 0.708 11.92 ± 0.861 12.01 ± 0.822

％ -1.12±2.490 0.15±1.585 0.76±2.837 -0.69±6.123 0.12±6.826

Puncture ginseng capsule 5.10g crude drug/kg 12.52 ± 0.961 12.17 ± 0.583 12.33 ± 1.055 12.65 ± 1.590 12.49 ± 1.417 12.28 ± 1.707

％ -2.63±4.057 -1.47±5.827 1.13±11.524 -0.14±10.040 -1.97±10.682

After the administration (min)

60 90 120 150 180 240

13.03±1.455 13.25±1.570 13.15±1.600 13.09±1.196 12.96±1.526 12.86±1.582

-1.28±4.971 0.39±7.003 -0.36±6.609 -0.79±5.312 -1.77±6.530 -2.54±7.908

12.62±1.006 12.73±1.062 12.35±0.893 12.34±0.970 12.29±1.053 12.05±1.190

-6.74±4.986 -5.84±6.717 -8.49±8.345 -7.89±8.648 -8?72±11.060 -10.55±11.559

12.09±0.791 11.88±0.927 11.78±0.886 12.00±0.917 11.99±1.217 19.51±1.201

-1.31±10.300 -2.88±12.698 -3.67±13.223 -1.91±12.335 -2.08±13.473 -0.17±9.582

11.89±0.922 11.74±0.992 11.85±0.942 11.63±0.878 11.43±0.906 11.25±0.876

-1.10±4.231 -2.35±5.237 -1.23±8.094 -3.27±3.752 -4.96±3.914 -6.46±2.398

12.24±1.482 12.17±1.921 12.11±1.857 11.52±1.559 11.55±1.482 11.71±1.637

-2.091±1.387 -2.95±12.613 -3.391±2.009 -8.17±8.561 -7.78±9.716 -6.584±10.186

Table 3. puncture ginseng capsule to the influence of left ventricular pressure (KPa) (X ± SD, n=6)

After the administration (nin)

Group dosed administration preceding 5 10 20 30 45

Normal control group----20.69 ± 1.201 20.53 ± 1.086 20.49 ± 1.113 20.84 ± 0.976 20.71 ± 1.046 20.64 ± 1.028

％ -0.73±1.644 -0.94±2.077 0.81±2.417 0.13±1.754 -0.19±0.850

XINKESHU PIAN 03.8g/kg 20.44 ± 0.632 20.13 ± 1.218 19.71 ± 1.096 19.60 ± 1.050 19.62 ± 1.006 19.57 ± 0.947

％ -1.54±4.657 -3.55±4.881 -4.08±5.232 -3?96±5.250 -4.20±4.560

Puncture ginseng capsule 1.28g crude drug/kg 19.62 ± 1.242 19.95 ± 1.434 19.57 ± 1.572 19.51 ± 1.159 19.62 ± 1.298 19.46 ± 0.891

％ 1.83±6.855 -0.14±6.975 -0.41±6.162 0.14±6.363 -0.52±6.892

Puncture ginseng capsule 2.55g crude drug/kg 19.62 ± 1.703 19.44 ± 2.375 19.46 ± 2.020 19.57 ± 1.560 19.44 ± 1.630 19.53 ± 1.455

％ -1.11±5.740 -0.84±4.252 -0.03±5.632 -0.62±7.801 -0.11±7.682

Puncture ginseng capsule 5.10g crude drug/kg 19.71 ± 0.547 19.89 ± 0.813 19.84 ± 1.589 20.33 ± 1.685 20.00 ± 1.526 19.57 ± 1.552

％ 0.91±3.178 0.63±6.935 3.14±7.743 1.47±7.373 -0.69±7.165

After the administration (min)

60 90 120 150 180 240

20.35±0.848 20.42±1.056 20.62±1.277 20.44±1.295 20.22±1.332 19.93±1.119

-1.53±2.454 -1.21±3.867 -0.30±3.610 -1.12±4.684 -2.25±3.765 -3.59±3.859

19.00±1.258 18.98±1.318 18.75±1.647 18.84±1.522 18.73±1.728 18.37±1.396

-7.01±6.146 -7.14±6.038 -8.18±8.504 -7.73±8.038 -8.28±8.934 -10.02±7.477

19.51±1.247 19.26±1.190 19.09±1.337 19.46±1.159 19.51±1.201 19.31±1.350

-0.14±10.055 -1.45±9.201 -2.30±10.264 -0.40±9.406 -0.17±9.582 -1.16±10.439

19.55±1.358 19.06±2.234 19.24±1.600 18.62±1.594 18.75±1.584 18.42±1.532

-0.05±6.039 -2.95±5.581 -1.80±4.431 -5.00±4.104 -4.33±3.543 -6.05±2.542

19.62±1.848 19.44±1.762 19.33±1.777 19.15±2.049 19.20±2.010 19.06±1.745

-0.46±8.832 -1.38±8.211 -1.96±8.018 -2.91±9.065 -2.70±8.813 -3.33±7.650

Table 4. puncture ginseng capsule to the influence of left chamber end diastolic pressure (KPa) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----0.71 ± 1.154 0.76 ± 1.105 0.80 ± 1.091 0.80 ± 1.179 0.73 ± 1.180 0.80 ± 1.093

％ 11.84±31.224 0.49±65.625 29.41±40.516 27.04±51.656 -31.10±142.96

XINKESHU PIAN 0.38g/kg 0.13 ± 0.469 0.11 ± 0.514 0.07 ± 0.460 0.11 ± 0.442 0.07 ± 0.429 0.05 ± 0.467

％ -3.23±51.599 -13.58±45.045 -10.97±48.562 2.04±68.426 -30.25±84.573

Puncture ginseng capsule 1.28g crude drug/kg 0.11 ± 0.614 0.11 ± 0.514 0.11 ± 0.503 0.20 ± 0.545 0.11 ± 0.503 0.29 ± 0.560

％ -15.13±16.873 -2.43±58.617 -12.21±14.332 -14.54±13.026 -29.30±45.326

Puncture ginseng capsule 2.55g crude drug/kg 0.22 ± 0.549 0.18 ± 0.581 0.18 ± 0.373 0.20 ± 0.462 0.18 ± 0.510 0.18 ± 0.518

％ -33.33±51.640 -21.68±40.211 -18.64±99.894 -18.30±40.217 -18.30±40.217

Puncture ginseng capsule 5.10g crude drug/kg 0.60 ± 0.693 0.71 ± 0.550 0.67 ± 0.456 0.71 ± 0.601 0.69 ± 0.561 0.62 ± 0.530

％ -41.76±95.491 -57.13±95.606 -37.91±69.854 -24.01±67.258 -32.56±58.994

After the administration (nin)

60 90 120 150 180 240

0.78±1.153 0.67±1.125 0.73±1.209 0.80±1.115 0.76±1.222 0.78±1.127

12.58±30.811 -10.84±59.653 29.07±72.212 -65.32±173.13 17.13±67.090 -18.28±64.253

0.02±0.410 0.11±0.575 0.09±0.411 0.07±0.437 0.09±0.481 0.04±0.489

-46.63±139.06 -37.14±E1683.972?-41.08±78.900?-35.66±80.702 -33.33±81.650 -59.92±123.14

0.14±0.456 0.02±0.538 0.00±0.484 0.02±0.500 0.07±0.452 0.00±0.497

-23.29±25.444 -4.39±69.448 -8.22±67.602 -19.38±34.111 -28.50±37.361 -22.08±40.324

0.15±0.408 0.20±0.512 0.18±0.444 0.16±0.467 0.20±0.454 0.24±0.410

-38.35±80.103 -18.30±40.217 -19.92±40.000?-19.92±40.000 -36.59±80.343 -19.92±40.000

0.60±0.519 0.58±0.575 0.60±0.551 0.62±0.564 0.67±0.562 0.58±0.482

-58.88±78.381 -31.28±38.642 -46.46±79.948?-34.90±54.167 -51.50±83.159 -59.35±78.381

Table 5. puncture ginseng capsule is right+and the influence of dp/dtmax (KPa/SD) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----382.13 ± 44.344 388.79 ± 61.037 388.79 ± 67.664 402.12 ± 61.037 391.01 ± 59.415 386.57 ± 46.176

％ 1.88±12.928 1.78±14.165 5.60±14.729 2.50±12.519 1.58±11.320

XINKESHU PIAN 0.38g/kg 477.66 ± 97.743 435.45 ± 64.574 431.00 ± 72.849 419.90 ± 51.108 426.56 ± 74.933 424.34 ± 89.784

％ -6.36±17.924 -7.63±17.159 -9.18±19.768 -8.12±20.565 -9.01±21.113

Puncture ginseng capsule 1.28g crude drug/kg 417.67 ± 86.662 399.90 ± 116.82 395.46 ± 110.14 377.68 ± 94.508 377.68 ± 88.689 379.91 ± 62.948

％ -5.16±14.592 -6.07±14.321 -9.87±13.126 -9.80±9.470 -8.43±6.894

Puncture ginseng capsule 2.55g crude drug/kg 417.67 ± 60.010 415.45 ± 49.681 415.45 ± 57.438 437.67 ± 44.941 444.33 ± 82.027 424.34 ± 78.372

％ -0.46±4.658 0.46±1.134 5.54±9.643 6.91±18.572 2.34±19.483

Puncture ginseng capsule 5.10g crude drug/kg 448.78 ± 98.918 433.23 ± 771.53 442.11 ± 85.319 451.00 ± 76.536 453.22 ± 101.17 415.45 ± 70.241

％ -2.13±12.285 -0.20±12.903 2.14±13.144 2.02±14.158 -6.24±9.426

After the administration (min)

60 90 120 150 180 240

393.24±54.474 382.13±55.710 284.35±56.816 388.79±67.664 371.02±57.438 362.13±62.191

3.17±11.747 0.25±12.811 0.67±11.340 1.47±11.057 -2.94±10.627 -5.45±10.684

406.57±71.908 391.01±72.360 371.02±88.589 386.57±82.172 384.35±74.656 368.80±109.81

-12.10±21.813 -14.59±26.544 -18.56±29.540 -15.72±27.154 -16.10±26.740 -19.82±28.737

377.68±60.010 373.24±47.691 371.02±43?331 373.24±47.691 364.35±43.536 357.69±43.331

-8.75±9.060 -9.04±13.535 -9.15±15.785 -8.87±15.207 -11.26±12.118 -12.93±11.672

437.67±79.273 41.101±57.438 422.12±56.345 399.90±60.794 402.16±49.459 384.35±50.876

5.28±17.708 -0.94±13.213 1.72±12.194 -3.89±10.369 -2.98±11.546 -7.35±11.517

442.11±100.61 431.00±119.43 422.12±108.18 422.12±113.63 417.67±121.20 411.01±107.44

-0.82±12.208 -4.291±34.25 -6.30±9.884 -6.63±9.153 -8.01±9.693 9.00±7.825

Table 6. puncture ginseng capsule is right-and the influence of dp/dtmax (KPa/SD) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----297.70 ± 71.867 311.03 ± 7908.32 322.14 ± 75.602 315.48 ± 77.115 311.03 ± 68.3836 322.14 ± 68.707

％ 4.30±6.326 8.93±11.136 6.01±6.546 5.25±8.199 8.95±7.313

XINKESHU PIAN 0.38g/kg 351.02 ± 86.251 366.58 ± 63.510 342.14 ± 40.138 346.58 ± 33.723 357.69 ± 56.187 353.25 ± 58.257

％ 7.43±19.151 1.37±21.227 3.45±25.062 5.90±23.562 5.76±30.086

Puncture ginseng capsule 1.28g crude drug/kg 293.26 ± 101.87 288.82 ± 97.834 286.60 ± 119.15 291.04 ± 101.31 282.15 ± 94.414 277.71 ± 79.720

％ -1.15±8.900 -4.18±13.697 -0.51±8.876 -3.20±8.682 -2.65±14.154

Puncture ginseng capsule 2.55g crude drug/kg 322.14 ± 48.000 326.59 ± 50.408 324.36 ± 51.741 339.92 ± 47.504 333.25 ± 69.521 326.59 ± 61.809

％ 1.77±10.763 0.80±7.510 6.11±10.301 3.24±12.856 1.29±12.740

Puncture ginseng capsule 5.10g crude drug/kg 295.48 ± 108.76 308.81 ± 102.71 313.26 ± 85.041 337.69 ± 96.001 319.92 ± 86.388 31992 ± 82.603

％ 7.24±18.583 10.74±17.967 19.18±20.350 13.03±18.410 13.67±21.010

After the administration (min)

60 90 120 150 180 240

317.70±84.482 322.14±89.784 328.81±82.027 326.59±85.873 331.03±80.165 328.81±79.832

7.10±19.633 7.87±15.123 10.44±12.929 9.23±9.927 11.69±17.304 11.03±17.684

344?36±61.037 339.92±75.288 331.03±73.698 333.25±62.523 326.59±66.249 322.14±59.861

1.97±21.374 0.34±26.590 -1.49±28.999 -0.33±30.931 -1.49±34.761 -3.72±28.447

273.27±68.361 275.49±72.849 264.38±54.97 275.49±62.333 277.71±68.707 284.37±60.599

-3.73±13.922 -2.81±19.760 -5.59±18.536 -1.03±25.249 -1.08±20.930 2.84±25.364

337.69±43.536 328.81±45.919 315.48±39.242 313.26±37.467 306.59±35.768 306.5±49.876

5.59±10.680 2.48±9.150 -1.62±6.213 -2.15±7.978 -4.03±5.778 -4.85±7.234

311.03±85.006 295.48±76.071 286.60±71.412 284..37±74.398 273.27±76.226 273.27±75.759

9.66±17.079 8.08±35.706 4.32±32.882 2.94±30.005 -2.24±24.249 -1.89±26.123

Table 7. puncture ginseng capsule to the influence of heart output (L/min) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----1.27 ± 0.108 1.31 ± 0.099 1.32 ± 0.112 1.32 ± 0.113 1.29 ± 0.094 1.31 ± 0.105

％ 2.75±4.088 3.69±2.840 3.50±4.940 1.70±3.523 3.14±4.920

XINKESHU PIAN 0.38g/kg 1.28 ± 0.109 1.30 ± 0.106 1.30 ± 0.110 1.31 ± 0.095 1.30 ± 0.107 1.29 ± 0.096

％ 1.39±3.485 1.59±2.300 2.07±2.845 1.25±3.544 1.02±2.876

Puncture ginseng capsule 1.28g crude drug/kg 1.28 ± 0.131 1.30 ± 0.123 1.28 ± 0.102 1.27 ± 0.124 1.30 ± 0.124 1.33 ± 0.121

％ 1.23±0.946 0.22±3.209 -1.12±1.873 1.25±1.777 3.35±1.467

Puncture ginseng capsule 2.55g crude drug/kg 1.27 ± 0.138 1.30 ± 0.149 13.1 ± 0.134 1.31 ± 0.129 1.29 ± 0.134 1.31 ± 0.156

％ 2.71±1.545 3.22±1.641 3.66±1.440 2.05±2.436 2.88±4.693

Puncture ginseng capsule 5.10g crude drug/kg 1.28 ± 0.121 1.31 ± 0.106 1.3 ± 0.101 1.30 ± 0.100 1.28 ± 0.128 1.27 ± 0.117

％ 1.79±1.791 1.44±2.321 1.45±3.093 -0.28±2.514 -0.72±2.559

After the administration (min)

60 90 120 150 180 240

1.27±0.099 1.26±0.091 121±0.116 1.23±0.104 1.21±0.118 1.22±0.094

0.26±4.724 -0.90±4.159 -4.63±2.518 -3.09±3.922 -4.61±4.511 -3.92±5.041

1.28±0.117 1.29±0.121 1.27±0.088 1.22±0.108 1.18±0.148 1.19±0.140

-0.16±2.589 0.51±3.279 -0.81±5.552 -5.06±2.830 -8.02±4.856 -7.05±5.478

1.27±0.106 1.23±0.101 1.23±0.110 1.24±0.098 1.23±0.106 1.20±0.087

-1.22±3.769 -4.37±2.499 -4.00±4.100 -3.24±4.761 -3.98±3.076 -5.87±5.990

1.31±0.112 1.26±0.147 1.23±0.164 1.24±0.150 1.19±0.136 1.22±0.114

3.48±5.520 -1.02±5.341 -3.59±3.515 -2.18±3.053 -6.44±4.166 -3.65±3.301

128±0.113 1.26±0.134 1.25±0.112 1.23±0.103 1.20±0.118 1.20±0.101

-0.45±3.145 -2.29±2.835 -2.89±3.660 -4.32±2.583 -6.38±2.245 -6.38±2.973

Table 8. puncture ginseng capsule is to myocardial flow (ml/100g.Influence min) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----108.72 ± 17.834 109.26 ± 21.077 109.24 ± 18.877 105.46 ± 17.018 106.34 ± 17.781 106.95 ± 20.088

％ 0.16±5.045 0.60±8.185 -2.84±5.123 -2.11±6.088 -1.83±5.683

XINKESHU PIAN 0.38g/kg 122.52 ± 31.346 120.54 ± 31.955 126.24 ± 31.850 121.35 ± 37.672 125.80 ± 42.139 135.21 ± 35.085

％ -1.79±4.041 3.25±3.320 -2.27±7.334 10.98±9.358 11.36±11.830▲

Puncture ginseng capsule 1.28g crude drug/kg 99.99+20.091 104.47 ± 21.467 102.35 ± 21.359 97.52 ± 22.039 105.34 ± 14.605 108.49 ± 24.529

％ 4.43±6.756 2.21±6.083 -2.90±5.989 6.71±9.166 8.06±9014▲

Puncture ginseng capsule 2.55g crude drug/kg 99.84 ± 15.666 100.67 ± 10.200 101.82 ± 12.371 100.38 ± 14.826 109.27 ± 12.934 113.92 ± 12.905

％ 1.80±8.037 2.59±5.571 0.74±2.532 10.30±8.937▲ 15.84±13.710▲

Puncture ginseng capsule 5.10g crude drug/kg 91.94 ± 13.248 96.51 ± 13.034 97.18 ± 13.779 96.92 ± 14.047 100.48 ± 9.706 104.88 ± 9.842

％ 5.31±6.689 5.89±6.515 5.74±8.142 10.27±10.249▲?15.45±153.19▲

After the administration (min)

60 90 120 150 180 240

101.09±17.823 101.49±18.588 95.71±17.217 96.09±19.422 98.33±20.071 97.09±13.890

-6.99±6.215 -6.67±7.432 -11.22±12.782 -10.77±15.095 -9.47±10.872 -10.17±7.447

132.34±29.790 130.96±27.105 115.80±23.205 112.15±29.874 109.11±28.776 99.53±24.784

10.42±17.818▲ 8.81±12.712▲ -3.12±15.801 -7.96±8.511 -9.96±12.349 -18.13±9.232

108.05±25.441 105.57±21.661 106.28±.23.154?102.04±17.847 102.47±17.134 99.67±21.621

7.45±10.314▲ 5.52±6.534▲ 6.09±10.666▲ 2.78±10.217 3.45±10.828 -0.16±13.934

117.99±14.442 109.03±17.477 109.48±15.388 95.54±14.151 93.93±14.623 92.17±14.387

19.93±17.984▲▲?10.18±14.493▲ 11.28±17.780▲?-3.53±11.014 -5.36±9.596 -6.14±16.791

108.61±12.576 111.82±12.912 97.70±15.433 92.33±15.431 88.89±15.208 85.82±18.914

19.44±16.622▲▲?23.04±18.059▲▲?6.94±14.598▲ 1.00±13.160 -2.70±13.413 -6.47±15.368

With the normal control group change percentage ▲ P＜0.05 ▲ ▲ P＜0.01

Table 9. puncture ginseng capsule to the influence of coronary resistance (Kpa/ml/100/min) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----0.12 ± 0.023 0.12 ± 0.026 0.12 ± 0.025 0.13 ± 0.022 0.13 ± 0.026 0.13 ± 0.027

％ 0.42±4.667 0.11±8.218 4.17±7.057 2.50±8098 2.09±6.834

XINKESHU PIAN 0.38g/kg 0.12 ± 0.030 0.11 ± 0.029 0.29 ± 8.218 0.1 2 ± 0 038 0.11 ± 0.039 0.10 ± 0.022

％ -1.45±5.670 -7.92±4.290 -1.67±6.639 5.84±9.333 -12.58±10.823▲

Puncture ginseng capsule 1.28g crude drug/kg 0.13 ± 0.036 0.12 ± 0.038 0.12 ± 0.041 0.13 ± 0.049 0.11 ± 0.023 0.12 ± 0.039

％ -7.09±13.533 -5.76±13.893 -1.98±14.243 -9.46±10.987 -8.32±10.054

Puncture ginseng capsule 2.55g crude drug/kg 0.12 ± 0.020 0.12 ± 0.014 0.12 ± 0.015 0.12 ± 0.019 0.11 ± 0.010 0.11 ± 0.012

％ -2.46±6.027 -2.12±5.676 0.04±1.583 -9.33±10.594 -12.32±11.402▲

Puncture ginseng capsule 5.10g crude drug/kg 0.14 ± 0.019 0.13 ± 0.016 0.13 ± 0.021 0.13 ± 0.022 0.12 ± 0.014 0.12 ± 0.012

％ -7.19±7.068 -6.50±9.337 -3.82±13.341 -8.72±13.188 -13.90±14.711▲

After the administration (min)

60 90 120 150 180 240

0.1340.027 0.13±0.030 0.14±0.017 0.14±0.020 0.13±0.022 0.13±0.021

6.46±8.140 8.03±10.475 14.06±16.695 14.16±21.764 9.45±11.133 8.92±10.319

0.10±0.024 0.10±0.024 0.11±0.026 ^* 0.12±0.032 0.12±0.0357 0.13±0.0426

-13.57±16080▲ -12.76±8.634▲▲ -3.96±13.108 0.26±?6.689 2.26±11.817 10.33±17.421

0.12±0.043 0.12±0.037 0.12±0.044 0.12±0.034 0.12±0.036 0.13±0.053

-7.40±13.032 -7.75±12.076▲ -8.42±14.943▲ -4.16±11.308 -4.75±13.640 -2.09±18.989

0.10±0.008 ^* 0.11±0.017 0.11±0.019 ^* 0.12±0.021 0.12±0.021 0.12±0.023

-15.86±13.669▲▲ -10.17±11.921▲ -9.00±18.974▲ 1.57±14.197 1.77±16.001 3.16±23.963

0.11±0.012 0.11±0.014 0.12±0.013 0.13±0.024 0.13±0.031 0.14±0.039

-16.69±15.040▲▲ -19.62±16.689▲▲?-9.07±10.132▲ -7.72±15.976 -3.39±19.208 2.37±22.087

Compare with the normal control group ^*P＜0.05 ^*P＜0.01

With the normal control group change percentage comparisons ▲ P＜0.05 ▲ ▲ P＜0.01

Table 10. puncture ginseng capsule to the influence of total peripheral resistance (Kpa/L/min) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----835.71 ± 121.01 817.51 ± 121.90 812.05 ± 141.55 816.50 ± 129.14 822.03 ± 125.30 809.65 ± 112.27

％ -2.12±4.760 -3.15±4.112 -2.32±5.146 -1.62±4.397 -3.02±1.853

XINKESHU PIAN 0.38g/kg 845.29 ± 28.369 807.45 ± 65.929 791.00 ± 51.056 792.82 ± 31.209 789.57 ± 58.878 807.20 ± 60.601

％ -4.51±6.609 -6.39±5.817 -6.14±4.252 6.66±4.993 -4.55±5327

Puncture ginseng capsule 1.28g crude drug/kg 773.26 ± 96.199 740.92 ± 148.99 742.74 ± 144.57 746.45 ± 160.12 735.66 ± 124.92 734.48 ± 82.260

％ -4.74±9.841 -4.52±9.181 -4.16±11.240 -5.23±6.750 -4.71±6.560

Puncture ginseng capsule 2.55g crude drug/kg 763.94 ± 84.286 736.09 ± 89.986 740.85 ± 78.551 741.01 ± 66.890 742.52 ± 83.070 717.17 ± 47.938

％ -3.70±3.012 -2.97±1.312 -2.78±3.175 -2.66±5.976 -5.52±7.956

Puncture ginseng capsule 5.10g crude drug/kg 787.27 ± 108.45 749.49 ± 71.730 761.00 ± 81.561 779.57 ± 98.009 784.78 ± 106.84 773.29 ± 109.85

％ -4.27±5.340 -2.78±7.057 -0.13±13.099 0.12±9.454 -1.17±11.249

After the administration (min)

60 90 120 150 180 240

823.49±126.46 849.10±144.08 878.89±173.78 860.91±167.75 865.40±165.50 849.41±146.13

-1.53±2.493 1.34±6.538 4.61±8.540 2.47±6.178 3.01±5.623 1.37±4.821

790.22±57.855 791.42±49.744 779.45±54.697 819.68±72.669 838.80±95.337 813.54±103.41

-6.53±5812 -6.36±5.258▲ -7.60±8.557▲ -2.81±10.435 -0.59±12.534 -3.47±14.420

767.43±70.617 776.95±55.796 766.83±38.635 776.34±52.809 780.56±67.131 784.39±92.667

-0.05±10.170 1.54±12.965 0.26±12.072 1.77±15.642 2.09±14.783 3.09±20.704

729.61±76.788 756.01±11.045 774.40±103.00 756?31±94.935 776.77±95.365 741.31±77.776

-4.17±7.086 -0.97±9.433 1.35±6.425 -1.02±4.768 1.69±4.833 -2.87±2.641

823.49±126.46 775.38±101.16 780.05±128.21 751.26±92.461 768.97±80.495 782.98±119.25

-1.49±12.702 -0.86±10.762 -0.29±13.650 -4.00±8.961 -1.57±9.299 0.10±13.701

Change percentage comparisons ▲ P＜0.05 with the normal control group

Table 11. puncture ginseng capsule to the influence of cardiac index (L/min/m2) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----2.01 ± 0.231 2.06 ± 0.211 2.08 ± 0.240 2.07 ± 0.222 2.03 ± 0.190 2.07 ± 0.211

％ 2.84±4.107 3.66±2.752 3.40±4.913 1.57±3.417 3.21±4.915

XINKESHU PIAN 0.38g/kg 2.11 ± 0.296 2.14 ± 0.295 2.14 ± 0.293 2.15 ± 0.300 2.13 ± 0.291 211 ± 0.314

％ 1.29±3.438 1.50±2.294 2.03±2.927 1.25±3.632 -0.16±2.695

Puncture ginseng capsule 1.28g crude drug/kg 2.10 ± 0.280 2.12 ± 0.268 2.10 ± 0.244 2.07 ± 0.269 2.12 ± 0.269 2.16+0.271

％ 1.27±0.848 0.29±3.200 -1.22±1.943 1.30±1.932 3.28±1.422

Puncture ginseng capsule 2.55g crude drug/kg 2.03 ± 0.235 2.08 ± 0.264 2.09 ± 0.251 2.10 ± 0.228 2.07 ± 0.225 2.08 ± 0.211

％ 2.60±1.581 3.09±1.743 3.45±1.395 1.92±2.403 2.79±4.694

Puncture ginseng capsule 5.10g crude drug/kg 2.00 ± 0.236 2.03 ± 0.204 2.02 ± 0.210 2.02 ± 0.199 1.99 ± 0.252 1.98 ± 0.240

％ 1.92±1.734 1.48±2.294 1.55±3.232 -0.30±2.565 -0.65±2.601

After the administration (min)

60 90 120 150 180 240

2.01±0.233 1.99±0.212 1.92±0.242 1.94±0.189 1.92±0.254 1.93±0.222

0.31±4.642 -0.83±4.204 -4.55±2.552 -3.001±3.976 -4.53±4.414 -3.99±4.948

2.11±0.314 2.12±0.295 2.08±0.269 2.00±0.262 1.94±0.299 1.96±0.306

-0.16±2.695 0.41±3.172 -0.99±5.646 -5.01±2.834 -8.02±4.746 -7.13±5.631

2.07±0.261 2.00±0.228 2.01±0.217 2.02±0.216 2.01±0.243 1.97±0.217

-1.23±3.863 -4.39±2.426 -3.97±4.262 -3.13±4.819 -3.95±3.124 -5.83±6037

2.09±0.199 2.00±0.269 1.95±0.239 1.99±0.283 1.90±0.249 1.95±0.223

3.31±5.584 -1.26±5.418 -3.70±3.502 -2.39±3.450 -6.52±4.262 -3.73±3.230

1.98±0.207 1.95±0.240 1.94±0.237 1.91±0.186 1.87±0.212 1.87±0.214

-0.42±3.129 -2.19±2.831 -2.75±3.800 -4.32±2.585 -6.28±2.201 -6.26±3.015

Table 12. puncture ginseng capsule to the influence of SI (ml/m2/beat) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----13.02 ± 2.311 13.29 ± 2.445 13.34 ± 2.636 13.48 ± 2.610 13.07 ± 2.370 13.14 ± 2.257

％ 2.03±4.705 2.14±3.982 3.29±6.561 0.43±6.932 1.05±2.197

XINKESHU PIAN 0.38g/kg 13.18 ± 2.787 13.75 ± 3.105 13.53 ± 3.003 13.64 ± 3.063 13.33 ± 2.838 13.34 ± 2.985

％ 4.20±7.085 2.53±6.129 3.41±8.428 1.32±8.4057.115 1.24±9.702

Puncture ginseng capsule 1.28g crude drug/kg 14.03 ± 1.191 14.55 ± 1.689 14.38 ± 1.495 14.31 ± 1.922 14.38 ± 1.536 14.42 ± 1.679

％ 3.57±5.699 2.41±5.533 1.81±8.227 2.40±5.169 2.64±5.849

Puncture ginseng capsule 2.55g crude drug/kg 13.54 ± 1.803 13.94 ± 2.034 14.09 ± 2.272 14.08 ± 2.278 13.74 ± 1.807 13.75 ± 1.719

％ 2.82±1.860 3.81±4.819 3.73±5.375 1.57±4.847 1.84±7.269

Puncture ginseng capsule 5.10g crude drug/kg 12.29 ± 1.471 1230 ± 1.373 12.19 ± 1.318 12.20 ± 1.368 11.83 ± 1.482 11.93 ± 1.329

％ 0.19±1.664 -0.62±3.266 -0.62±1.962 -3.69±3.526 -2.81±2.307

After the administration (min)

60 90 120 150 180 240

1268±2.095 12.53±2.068 11.95±2.257 12.10±2.172 12.02±2.299 12.09±2.020

-2.47±3.375 -3.38±6.056 -8.19±6.139 -7.04±5.689 -7.79±5.043 -6.90±5.902

13.22±3.135 13.40±3.056 13.38±3.486 12.82±3.205 12.47±3.194 12.55±3.046

0.14±10.546 1.82±12.420 1.17±13.392 -3.24±9.530 -5.72±13.071 4.97±12.588

13.87±1.353 13.79±1.365 13.72±1.219 13.78±1.007 13.77±1.345 13.54±0.882

-11.4±4.943 -1.55±8.320 -1.91±9.372 -1.59±5.966 -1.76±7.052 -3.30±5.066

13.57±2.067 13.20±1.941 12.98±1.543 13.14±1.894 12.67±2.006 13.14±1.865

0.50±10.909 -2.21±10.530 -3.75±6.560 -2.92±4.826 -6.58±6.057 -2.92±6.521

11.92±1.407 11.59±1.273 11.54±1.301 11.27±0.729 10.99±0.883 11.19±1.104

-2.95±2.561 -5.57±2.433 -5.85±6.133 -7.70±5.939 -10.18±4.615 -8.67±4.982

Table 13. puncture ginseng capsule to the influence of left chamber work done (l/min/m2.kPa) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----0.36 ± 0.044 0.37 ± 0.39 0.37 ± 0.048 0.37 ± 0.047 0.36 ± 0.038 0.37 ± 0.041

％ 3.54±5.203 4.40±8.640 4.47±9.415 1.42±9.463 3.30±10.273

XINKESHU PIAN 0.38g/kg 0.39 ± 0.082 0.39 ± 0.084 0.38 ± 0.078 0.38 ± 0.080 0.37 ± 0,078 0.38 ± 0.076

％ -2.30±3.898 -3.70±2.236 -2.85±3.851 -5.41±3.309 -4.07±2.809

Puncture ginseng capsule 1.28g crude drug/kg 0.35 ± 0.070 0.34 ± 0.072 0.34 ± 0.073 0.33 ± 0.081 0.34 ± 0.078 0.35 ± 0.054

％ -2.59±10.866 -3.47±12.914 -6.24±11.166 -2.95±8.968 2.10±10.034

Puncture ginseng capsule 2.55g crude drug/kg 0.33 ± 0.054 0.33 ± 0.056 0.34 ± 0.052 0.34 ± 0.050 0.33 ± 0.052 0.34 ± 0.045

％ 0.98±3.731 3.68±4.327 4.29±3.655 1.37±7.363 3.14±7.104

Puncture ginseng capsule 5.10g crude drug/kg 0.34 ± 0.027 0.33 ± 0.027 0.34 ± 0.038 0.35 ± 0.059 0.34 ± 0.060 0.33 ± 0.061

％ -0.94±3.075 -0.16±4.972 2.55±10.697 -0.44±11.006 -2.55±10.979

After the administration (min)

60 90 120 150 180 240

0.36±0.057 0.36±0.046 0.34±0.44 0.34±0.034 0.34±0.051 0.33±0.054

-0.95±9.861 -0.11±9.726 -4.52±6.553 -4.16±8.126 -5.93±9.838 -6.72±12.478

0.36±0.066 0.37±0.072 0.35±0.054 0.34±0.055 0.33±0.064 0.32±0.067

-7.51±5.593 -6.51±8.585 -10.47±11.081 13.36±8.528 -16.26±12.268 -18.21±12.270

0.34±0.053 0.32±0.058 0.32±0.058 0.33±0.051 0.33±0.067 0.31±0.058

-2.05±12.309 -6.36±14.592 -7.20±16.247 -4.80±12.667 -5.64±14.500 -9.35±12.838

0.34±0.045 0.32±0.045 0.31±0.042 0.31±0.050 0.30±0.049 0.30±0.043

2.15±7.088 -4.20±4.841 -4.26±10.208 -4.97±5.994 -10.53±6.978 -9.81±5.653

0.33±0.064 0.32±0.073 0.32±0.064 0.30±0.048 0.29±0.053 0.29±0.046

-2.6±11.155 -5.22±15.608 -6.51±13.217 -12.71±l9.416 -14.32±10.008 -13.19±8.632

Table 14. puncture ginseng capsule to the influence of cardiac output (ml/beat) (X ± SD, n=6)

After the administration (min)

Group dosed administration preceding 5 10 20 30 45

Normal control group----8.28 ± 1.497 8.45 ± 1.616 8.49 ± 1.706 8.59 ± 1.726 8.34 ± 1.592 8.37 ± 1.508

％ 1.94±4.754 2.18±4.052 3.42±6.705 0.57±7.087 1.00±5.217

XINKESHU PIAN 0.38g/kg 8.01 ± 1.555 8.35 ± 1.585 8.22 ± 1.576 8.26 ± 1.437 8.07 ± 1,329 8.07 ± 1.327

％ 4.34±7.172 2.64±6.131 3.46±8.408 1.28±8.348 1.32±9.764

Puncture ginseng capsule 1.28g crude drug/kg 8.62 ± 0.763 8.94 ± 1.056 8.82 ± 0.924 8.80 ± 1.219 8.83 ± 1.032 8.86 ± 1.083

％ 3.55±5.819 2.36±5.681 1.91±8.366 2.33±5.109 2.68±5.867

Puncture ginseng capsule 2.55g crude drug/kg 8.44 ± 0.807 8.69 ± 0.887 8.77 ± 0.872 8.77 ± 0.918 8.58 ± 0.878 8.60 ± 0.993

％ 2.90±1.863 3.93±4.809 3.91±5.377 1.71±4.862 1.93±7.241

Puncture ginseng capsule 5.10g crude drug/kg 7.91 ± 0.853 7.91 ± 0.786 7.84 ± 0.683 7.85 ± 0.760 7.61 ± 0.775 7.67 ± 0.710

％ 0.07±1.732 -0.68±3.259 -0.72±1.930 -3.65±3.554 -2.89±2.323

After the administration (min)

60 90 120 150 180 240

8.06±1.351 7.97±1.332 7.60±1.411 7.7±1.440 7.63±1.421 7.69±1.275

-2.48±3.512 -3.41±6.407 -8.26±6.085 -7.13±5.738 -7.85±5.138 -6.80±5.951

8.00±1.498 8.13±1.512 8.11±1.707 7.76±1.637 7.56±1.755 7.61±1.564

0.17±10.497 1.94±12.455 1.38±13.349 -3.26±9.567 -5.71±13.124 -4.86±12.427

8.51±0.795 8.48±0.919 8.44±0.967 8.47±0.867 8.46±0.884 8.31±0.607

-1.14±5.191 -1.56±8.255 -1.93±9271 -1.70±5.957 -1.81±7.020 -3.34±5.081

8.49±1.138 8.25±0.969 8.12±0.876 8.21±0.820 7.90±1.017 8.20±0.909

0.64±10.800 -1.99±10.516 -3.64±6.695 -2.71±4.730 -6.52±6.094 -2.85±6.619

7.68±0.875 7.45±0.751 7.41±0.599 7.26±0.395 7.07±0.467 7.19±0.510

-2.97±2.469 -5.72±2.448 -5.98±6.119 -7.71±5.925 -10.27±4.683 -8.78±4.966

Table 15. puncture ginseng capsule to the influence of anesthetized dog myocardial oxygen consumption (ml/100g/min) (X ± SD, n=6)

After the administration (min)

Before the group dosed administration

30 45 90 150 240

Normal control group----7.04 ± 1.842 7.29 ± 2.065 7.35 ± 1.847 7.25 ± 1.726 6.57 ± 0.397 6.94 ± 0.849

％ 3.13±5.632 4.79±7.857 3.98±12.825 -4.01±19.837 1.82±16.526

XINKESHU PIAN 0.38g/kg 7.73 ± 1.275 7.10 ± 1.912 6.37 ± 1.026 6.23 ± 1.619 6.90 ± 1.704 6.72 ± 0.880

％ -9.24±10.158▲ -16.79±12.256▲▲ -18.46±19.602▲ -10.71±23.085 -11.25±16.546

Puncture ginseng capsule 1.28g crude drug/kg 6.14 ± 1.625 5.53 ± 1.736 5.28 ± 2.068 6.48 ± 1.993 6.56 ± 11.25 7.15 ± 2.268

％ -130.00±12.623▲?-16.35±19.046▲ 4.72±8.287 6.06±12.644 14.37±18.065

Puncture ginseng capsule 2.55g crude drug/kg 6.83 ± 2.042 5.83 ± 2.440 5.61 ± 2.961 5.90 ± 2.369 6.66 ± 2.947 7.07 ± 2.567

％ -15.46±16.192▲ -19.96±22.941▲ -13.44±25.264 -3.47±31.023 3.41±15.662

Puncture ginseng capsule 5.10g crude drug/kg 6.07 ± 2.136 5.35 ± 2.042 4.25 ± 2.748 ^*4.38 ± 2.722 5.76 ± 2.110 5.95 ± 1.455

％ -11.80±1.327▲ -33.80±26.057▲▲ -33.35±24.663▲▲ -4.11±31.494 2.90±22.403

With the normal control group change percentage comparisons ▲ P＜0.05 ▲ ▲ P＜0.01

Compare * P＜0.05 with the normal control group

Table 16. puncture ginseng capsule to anesthetized dog myocardial oxygen consumption index (inferior/min.KPa) influence (and X ± SD, n=6)

After the administration (min)

Before the group dosed administration

30 45 90 150 240

Normal control group----20.78 ± 4.662 21.20 ± 5.692 21.32 ± 5.416 21.59 ± 5.963 21.68 ± 5.869 21.04 ± 5.833

％ 1.59±9.519 2.36±8.532 3.30±9.353 3.69±6.928 0.69±9.900

XINKESHU PIAN 0.38g/kg 22.09 ± 3.640 20.90 ± 3.504 21.26 ± 32183.449 20.58 ± 3.253 20.06 ± 3.715 19.52 ± 4.331

％ -5.14±8.387 -3.24±9.927 -6.23±11.793 -8.92±12.077 -11.44±16.741

Puncture ginseng capsule 1.28g crude drug/kg 18.39 ± 2.259 17.55 ± 3.314 18.16 ± 1.757 17.33 ± 2.669 17.67 ± 2.457 17.56 ± 2.780

％ -4.60±11.333 -0.38±11.500 -5.46±11.035 -3.57±9.369 -4.17±11.448

Puncture ginseng capsule 2.55g crude drug/kg 18.07 ± 1.596 18.00 ± 1.784 18.33 ± 2.653 17.97 ± 2.830 17.66 ± 2.570 16.82 ± 2.004

％ -0.25±7.342 1.37±10.115 -0.80±10.189 -2.38±9.565 -6.92±6.760

Puncture ginseng capsule 5.10g crude drug/kg 20.33 ± 1.295 21.03 ± 2.506 20.42 ± 2.999 20.46 ± 3.030 19.45 ± 2.776 19.54 ± 2.703

％ 3.36±9.551 0.29±11.436 0.84±15.295 -4.36±11.635 -3.90±11.610

Table 17. puncture ginseng capsule to the influence of anesthetized dog cardiac muscle oxygen uptake rate (%) (X ± SD, n=6)

After the administration (min)

Before the group dosed administration

30 45 90 150 240

Normal control group----33.96 ± 4.720 36.34 ± 6.237 36.33 ± 3.497 37.41 ± 4.216 37.50 ± 5.976 39.16 ± 3.316

％ 6.75±8.103 8.49±5.161 11.05±12.709 10.64±11.227?16.24±10.367

XINKESHU PIAN 0.38g/kg 34.92 ± 7.640 32.67 ± 9.021 29.57 ± 8.446 ^*27.63 ± 6.859 ^*33.68 ± 6.223 37.95 ± 8.889

％ -7.53±11.224▲ -20.98±12.870▲▲▲ -20.31±13.134▲▲ -1.46±18.955?9.05±10.609

Puncture ginseng capsule 1.28g crude drug/kg 32.24 ± 5.917 28.25 ± 8.042 26.06 ± 7.922 ^*32.34 ± 5.555 33.94 ± 6.539 3742 ± 7.126

％ -13.59±10.386▲▲ -20.52±11.166▲▲▲ 0.72±6.063 5.52±10.063 16.13±10.490

Puncture ginseng capsule 2.55g crude drug/kg 35.90 ± 9.649 28.84 ± 10.560 26.64 ± 12.083 30.02 ± 10.749 37.19 ± 13.497 40.88 ± 11.943

％ -20.82±9.678▲▲▲ -28.09±12.923▲▲▲ -17.69±15.379▲▲ 2.19±26.436 13.72±10.938

Puncture ginseng capsule 5.10g crude drug/kg 34.83 ± 10.603 29.49 ± 10.465 22.82 ± 12.878 ^*22.02 ± 12.068 ^*34.54 ± 12.706 38.37 ± 10.875

％ -16.23±6.479▲▲▲ -38.39±18.015▲▲▲ -40.09±19.696▲▲▲?-0.83±26.917?11.10±10.866

Compare * P＜0.05 with the normal control group

With the normal control group change percentage comparisons ▲ P＜0.05 ▲ ▲ P＜0.01 ' ▲ ▲ ▲ P＜0.001

Table 18. puncture ginseng capsule to the influence of myocardial infarction dog degree of ischemia (∑-SDT, Mv) (X ± SD, n=6)

After the administration (min)

Ligation 5 10 20 30 45 60 before the group dosed administration

Normal control group---9.83 ± 2.858 229.50 ± 43.528 229.83 ± 45.613 225.17 ± 41.233 224.00 ± 44.032 222.83 ± 30.486 227.50=26.644 217.17=41.902

％ 2420.0±987.01?2389.5±848.01?2352.4±862.56?2386.4±1084.6?2332.6±848.33 2385.7＝844.35 2271.2＝903.60

XINKESHU PIAN 0.38g/kg 8.83 ± 3.545 218.17 ± 57.031 218.00 ± 34.246 193.83 ± 48.705 178.50 ± 53.399 169.83 ± 54.587 153.67=63.551 ^*154.67=26.470 ^*

％ 2872.2±1866.6?2924.8±1916.7?2390.1±966.61?2274.6±1096.0?2281.0±1553.7 2094.5＝1624.9 2118.6＝1547.6

Puncture ginseng capsule 1.28g crude drug/kg 9.33 ± 1.366 239.83 ± 28.074 241.00 ± 20.698 232.33 ± 20.559 212.83 ± 39.963 212.67 ± 31.848 202.17=32.981 177.000=9.466 ^*

％ 2530.6±597.78?2554.9±641.51?24638±652.75 2259.0±731.17?2257.9±729.60 2140.8＝727.08 1836.9＝358.48

Puncture ginseng capsule 2.55g crude drug/kg 9.17 ± 1.835 186.33 ± 56.842 177.67 ± 48.591 178.83 ± 46.932 177.17 ± 54 455 163.83 ± 36.296 ^*167.50=41.128 ^*146.00=36.840 ^*

％ 1980.2±658.73?1894.3±599.68?1892.9±513.60?1905.4±719.13?1749.4±548.31 181.14＝672.04 1586.2±662.43

Puncture ginseng capsule 5.10g crude drug/kg 9.00 ± 1.789 229.33 ± 24.031 208.33 ± 59.500 180.83 ± 34.149 163.83 ± 52.151 157.00 ± 53.047 ^*156.00=53.123 ^*137.00=40.978 ^*

After the administration (min)

90 120 150 180 240 300 360

214.17±33.831 202.00±24.747 186.50±16.084 188.00±22.645 167.00±27.218 166.50＝19.766 161.67＝27.833

2257.6±932.34 2109.2±766.54 1940.7±661.84 1940.6±627.99 1740.8±710.50 1726.0?664.23 1690.5?722?14

159.17±15.105 ^** 161.33±13.530 ^**?160.67±16.083 ^* 157.67±9.185 ^* 144.00±7.797 146.50＝15.643 142.83＝14.331

2167.7±1562.2 2206.6±1632?7 2169.1±1524.5 2151.9±1577.4 1911.9±1273.4 2075.9＝1762.3 1968.7＝1516.4

168.17±36.739 ^* 165.17±26.821 ^* 154.83±29.315 ^*?153.33±27.097 ^* 163.17±27.154 147.67＝33.417 142.50＝29.201

1773.5±680.84 1725.8±557.86 1593.2±421.24 1584.2±451.26 1690.5±450.45 1524.7＝503.68 1483.1＝550.61

138.33±42.179 ^** 132.33±33.423 ^**?135.50±±37.967 ^*139.67±43.205 ^* 121.33±44.832 128.17＝43.490 118.17＝62.262

1496.2±675.03 1427.8±595.04 1468.8±651.51 1525.4±724.23 1325.8±724.98 1406.7＝721.78 1298.6＝875.90

127.00±43.174 ^** 138.33±33.494 ^**?125.17±39.992 ^**?133.50±47.175 ^* 131.33±40.781 138.17＝32.975 132.67＝19.294

1346.0±537.04 1466.9±426.77 133.13±533.65 1429.5±638.08 1413.2±580.39 1489.6＝492.73 1408.9＝262.88

Compare * P＜0.05**P＜0.01 with the normal control group

Table 19. puncture ginseng capsule to the influence (N-SDT, point) of myocardial infarction dog ischemia scope (X ± SD, n=6)

After the administration (min)

Ligation 5 10 20 30 45 60 before the group dosed administration

Normal control group----2.83 ± 0.753 21.33 ± 1.506 21.17 ± 1.602 21.00 ± 1.673 20.67 ± 1.862 21.33 ± 1.033 21.17 ± 0.983 20.50 ± 1.871

％ 700.00±228.76?697.22±243.68?691.67±246.70 684.72±270.00?705.56±243.51 693.06±215.41 67500±248.05

XINKESHU PIAN 0.38g/kg 3.17 ± 1.472 18.17 ± 3.764 19.50 ± 1.049 18.83 ± 1.835 18.00 ± 2.966 20.17 ± 0.983 18.17 ± 2.317 ^*17.33 ± 1.633 ^*

％ 634.72±527.79?737.50±648.03?698.33±557.51 609.72±378.68?766.11±648.34 613.33±380.15 603.61±433.76

Puncture ginseng capsule 1.28g crude drug/kg 3.33 ± 0.816 20.83 ± 1.602 21.50 ± 1.225 20.67 ± 2.160 20.17 ± 1.941 21.00 ± 0.894 19.33 ± 2.338 17.83 ± 1.722 ^*

％ 570.83±233.56?590.28±226.84?551.39±159.72?533.33±142.69?568.06±188.74 520.83±215.36 462.50±143.06

Puncture ginseng capsule 2.55g crude drug/kg 3.33 ± 0.816 20.50 ± 1.517 20.33 ± 1.751 20.50 ± 1.761 20.00 ± 2.449 18.83 ± 2.041 ^*18.50 ± 2.168 ^*17.00 ± 2.966 ^*

％ 558.33±228.95?552.78±230.50?561.11±237.27?551.39±257.68 509.72±224.25 487.50±165.47 440.28±165.03

Puncture ginseng capsule 5.10g crude drug/kg 3.00 ± 1.414 20.00 ± 1.414 18.83 ± 2.401 19.50 ± 2.665 17.83 ± 4.956 16.50 ± 4.889 ^*15.50 ± 4.416 ^*16.17 ± 4.355 ^*

％ 763.89±551.81?722.22±533.19?718.33±447.13?646.94±404.78 573.89±339.15 502.78±243.68 547.22±302.29

After the administration (min)

90 120 150 180 240 300 360

20.50±1.378 20.50±1.517 19.67±0.516 18.50±2.258 17.00±4.000 16.50±2.739 16.00±3.847

673.61±241.55 666.67±210?03 636.11±195.62 588.89±189.05 543.06±255.19 520.83±212.38 502.78±232.96

18.50±1.517 ^* 17.50±1.871 ^* 18.17±1.472 ^* 16.17±1.169 ^* 13.33±1.211 14.67±3.445 13.50±3.564

712.78±664.20 633.06±512.14 650.28±500.27 608.89±568.41 475.83±466.27 591.67±668.14 542.22±618.84

17.00±2.366 ^* 17.00±2.683 ^* 17.17±2.229 ^* 15.33±1.211 ^* 16.83±1.169 16.17±2.401 15.83±1.329

431.94±119.07 436.11±146.60 441.67±142.11 391.67±156.61 433.33±139.34 413.89±151.08 401.39±131.70

15.50±3.843 ^* 15.50±3.450 ^* 17.17±2.483 ^* 15.00±2.366 14.33±4.412 14.50±4.806 13.83±6.274

395.83±179.19▲ 398.61±182.15▲ 445.83±160.36 370.83±114.96 355.56±170.84 359.72±183.82 347.22±220.71

15.50±5.010 ^* 15.17、3.817 ^* 15.33±4.546 ^* 14.00±3.406 ^* 13.67±4.502 14.67±4.082 15.17±4.401

526.39±314.40 518.89±298.60 531.11±339.55 494.17±348.26 436.94±240.26 503.89±302.14 524.44±327.60

Compare * P＜0.05 with the normal control group

Change percentage comparisons ▲ P＜0.05 with the normal control group

Table 20. puncture ginseng capsule to the influence of myocardial infarction dog myocardial infarction area (X ± SD, n=6)

(%) infraction/left heart (%) of group dosage infraction/whole-heartedly

The normal control group---14.02 ± 1.236 19.45 ± 2.567

XINKESHU PIAN 0.38g/kg 12.27 ± 1.433 ^*15.97 ± 1.748 ^*

Puncture ginseng capsule 1.28g crude drug/kg 12.47 ± 1.423 16.65 ± 2.118

Puncture ginseng capsule 2.55g crude drug/kg 11.81 ± 1.294 ^*15.81 ± 1.892 ^*

Puncture ginseng capsule 5.10 crude drug g/kg 11.33 ± 1.061 ^*15.38 ± 1.117 ^*

Annotate: with the relatively * P＜0.05**P＜0.01 as follows summary of normal control group

Table 21. puncture ginseng capsule to the influence of ASDT (IU/L) in the myocardial infarction dog serum (X ± SD, n=6)

After the preceding administration of group dosed administration

(360min)

The normal control group---147.33 ± 32.776

35.83±12.884

％ 345.66±141.48

XINKESHU PIAN 0.38g/kg 107.33 ± 16.777 ^*

31.33±5.680

％ 248.82±66.229

Puncture ginseng capsule 1.28g crude drug/kg 112.83 ± 24.943

37.33±10.367

％ 221.14±113.44

Puncture ginseng capsule 2.55g crude drug/kg 105.50 ± 12.645 ^*

37.17±12.937

％ 212.84±117.72

Puncture ginseng capsule 5.10 crude drug g/kg 104.83 ± 13.862 ^*

36.83±11.286

％ 207.27±94.646

Table 22. puncture ginseng capsule to the influence of CPK (IU/L) in the myocardial infarction dog serum (X ± SD, n=6)

After the preceding administration of group dosed administration

(300min)

The normal control group---180.17 ± 9.642 2012.7 ± 151.72

％ 1021.0±122.03

XINKESHU PIAN 0.38g/kg 171.33 ± 29.864 1647.0 ± 262.52 ^*

％ 881.55±213.28

Puncture ginseng capsule 1.28g crude drug/kg 179.00 ± 17.573 1786.8 ± 358.56

％ 891.14±135.42

Puncture ginseng capsule 2.55g crude drug/kg 167.50 ± 31.634 1464.5 ± 411.80 ^*

％ 807.14±331.88

Puncture ginseng capsule 5.10g crude drug/kg 161.17 ± 20.163 1743.3 ± 122.03 ^*

％ 989.53±156.45

Table 23. puncture ginseng capsule to the influence of LDH (IU/L) in the myocardial infarction dog serum (X ± SD, n=6)

After the preceding administration of group dosed administration

(300min)

The normal control group---134.00 ± 10.696 178.17 ± 18.999

％ 32.90±8.297

XINKESHU PIAN 0.38g/kg 124.17 ± 23.490 160.83 ± 33.529

％ 29.37±10.006

Puncture ginseng capsule 1.28g crude drug/kg 143.17 ± 10.008 180.83 ± 13.182

％ 26.35±4.164

Puncture ginseng capsule 2.55g crude drug/kg 132.33 ± 21.824 171.00 ± 19.829

％ 30.28±8.926

Puncture ginseng capsule 5.10g crude drug/kg 124.50 ± 25.867 158.33 ± 29.710

％ 28.08±13.477

Claims (4)

1, a kind of Chinese medicine preparation for the treatment of cardiovascular disease is characterized in that being made by ratio of weight and the number of copies by following medicine:

Fructus Tribuli 60-5, Stem and leaf of Radix Ginseng 2-30

Radix Salviae Miltiorrhizae 10-45, Borneolum Syntheticum 0.05-0.8.

2, Chinese medicine preparation according to claim 1, its preferred weight proportioning is:

Fructus Tribuli 30, Stem and leaf of Radix Ginseng 10,

Radix Salviae Miltiorrhizae 15, Borneolum Syntheticum 0.1.

3,, it is characterized in that said medicament is a said dosage form on any pharmaceutics according to claim 1,2 described Chinese medicine preparation.

4, a kind of production technology for the treatment of the Chinese medicine preparation of angina pectoris may further comprise the steps: get Radix Salviae Miltiorrhizae and all be ground into fine powder or partly beat powder, partly or entirely Radix Salviae Miltiorrhizae adds alcohol reflux, and concentration of alcohol is between the 10-90%; Stem and leaf of Radix Ginseng and Fructus Tribuli extracting in water extract 1-6 time, and concentrating under reduced pressure adds ethanol precipitation, concentration of alcohol 50%-90%; Filter, decompression filtrate recycling ethanol is not to there being the alcohol flavor, and behind the thin up, filtrate is handled the good macroporous adsorptive resins of balance earlier by giving, colourless with washed with de-ionized water to effluent, discard cleanout fluid, use the 50-90% ethanol elution, collect eluent, collect 70% ethanol elution, concentrating under reduced pressure is concentrated into relative density 1.10 (50 ℃), spray drying; With above-mentioned Radix Salviae Miltiorrhizae medicated powder or Radix Salviae Miltiorrhizae extract and appropriate amount of starch mixing, the reuse cover grinds method and Borneolum Syntheticum mixing, granulates, and makes granule, promptly gets medicine of the present invention.