CN1513418A - Heparin zinc vacuum blood-holding tube, and technique for preparing heparin zinc - Google Patents
Heparin zinc vacuum blood-holding tube, and technique for preparing heparin zinc Download PDFInfo
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- CN1513418A CN1513418A CNA031284000A CN03128400A CN1513418A CN 1513418 A CN1513418 A CN 1513418A CN A031284000 A CNA031284000 A CN A031284000A CN 03128400 A CN03128400 A CN 03128400A CN 1513418 A CN1513418 A CN 1513418A
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- heparin
- zinc
- heparin zinc
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Abstract
A vacuum blood taking tube and the heparin zinc prearranged in said vacuum blood taking tube are disclosed. A process for preparing heparin zinc includes dissolving heparin sodium in water, exchanging with exchange resin, eluting with distiolled water, and low-temp drying. Its advantages are easy recognizing and operation, and high examining quality.
Description
Technical field
The invention belongs to the blood test field, a kind of specifically heparin zinc vacuum is contained blood vessel and heparin zinc preparation technology thereof.
Technical background
The hemocyte check is meant the count detection analysis of blood middle leukocytes (WBC), erythrocyte (RBC), platelet (PLT), hemoglobin (HGB) and related data, also claims blood routine examination.Blood routine examination is not only the various hemopathic main foundations of diagnosis, and to the diagnosis of other systemic disease with differentiate and also can provide many important informations, is one of the most frequently used in the clinical medical inspection, most important substance.The most primary means of blood routine examination are by the artificial microscopy of microscope, development along with preclinical medicine, the application of high science and technology, blood cell analyzer have become the important means that the replacement microscopy carries out routine analysis of blood, especially the blood analyser of band classification.
No matter be microscopy, also be to use blood analyser, obtain reliable and stable, the accurate data of blood routine examination, prevent the judgement that the clinic diagnosis people makes mistake, laboratory inspection will take into full account the multiple influence factor who influences in the blood routine examination, and strictness is controlled.
Accurate in order to obtain, reliable assay must obtain high-quality specimen.High-quality specimen is the first step of high-quality check.The most basic requirement of the high-quality specimen that the intact form that guarantees every cell in the blood preparation is used as blood routine examination.(selecting from " medical instrument and reagent " second phase in 2000)
Vacuum test tube is used for blood preparation and is captured in developed country's existing decades of history, also begins over past ten years to promote the use of in China.Because the blood sample of blood routine examination must be handled through the anticoagulant anticoagulant, in order to reduce operation sequence, guarantee quality inspection, the vacuum test tube (Sheng blood vessel) of anticoagulant has appearred presetting in test tube.The kind of anticoagulant is a lot of at present, and character is different, generally carries out different selections according to the difference of blood test purpose, could obtain expected effect and the purpose that reaches oriented detection.The existing coagulant that presets in vacuum test tube has: ammonium oxalate, potassium oxalate, double oxalate, fluorinated sodium oxalates, EDTA disodium, EDTA dipotassium, EDTA tripotassium, heparin sodium, Lithium acid heparin.But test tube quantity is various, medical personnel's complicated operation, and phenomenon with wrong test tube may appear, thus influence quality inspection.
Summary of the invention
The object of the present invention is to provide a kind of existing multiple anticoagulant test tube commonly used that replaces, simplify the blood test kind, the heparin zinc vacuum that is beneficial to identifying operation is contained blood vessel and heparin zinc preparation technology thereof.
Another object of the present invention provides a kind of preparation technology who is preset in the heparin zinc in the test tube.
The present invention realizes that the technical scheme of its goal of the invention is as follows: a kind of heparin zinc vacuum is contained blood vessel, presets heparin zinc in the vacuum blood vessel.The consumption that is preset in the heparin zinc in the standard vacuum test tube is 1-2mg.
A kind of method of production heparin zinc is carried out ion exchange with heparin sodium by the porous type cation exchange resin and is obtained heparin zinc; Concrete grammar is heparin sodium to be added water dissolve the back exchanger resin exchange of crossing fully, uses the distilled water eluting then, and cold drying gets required heparin zinc.
Used heparin sodium molecular weight is about 15000,1,100,000 medical units; Used exchanger resin is acid type polystyrol cation exchange resin.
General commonly used anticoagulant as: the anticoagulant principle of structure rafter acid trisodium, Disodium oxalate., double oxalate, disodiumedetate, ethylenediamine tetraacetic acid,dipotassium salt, ethylenediaminetetraacetic acid tripotassium all is to rely on the cascade theory principle that blood circle is generally acknowledged, form sequestration thing or precipitate with the calcium ion in the blood, make thrombinogen transform the effect that becomes thrombin thereby calcium ion necessary in the coagulation process is lost, reach the anticoagulant purpose.Different with above-mentioned various anticoagulant mechanism is heparin.Heparin extensively is present in the blood vessel mastocyte and basophilic granulocyte granule of nearly all when injected organism tissues such as animal lung, liver, splenomegaly enteral skin, it is a kind of mucopolysaccharide of sulfur acid group, molecular weight 15000, heparin can be strengthened antithrombase deactivation serine protease, form thereby have the thrombin of prevention, to multiple anticoagulations such as antithrombase and prevention platelet aggregations.Therefore heparin has the anticoagulant ability by force, does not influence blood cell volume, is difficult for advantages such as haemolysis.But the dissolubility of heparin in water is very little, is not suitable for the use on the clinical medical inspection.Therefore, generally heparin is made Calciparine/sodium salt at present and can be applicable to medical test clinically.But heparin sodium disturbs the test of the sodium ion in the important test electrolyte in the check of biochemical serum, generally in dielectric test, need to carry out twice, for addressing this problem, developed country has not adopted heparin sodium as anticoagulant at present, and adopts Lithium acid heparin to replace heparin sodium, in the check because of serum biochemistry, dielectric measurement is not contain lithium ion, so do not influence the test of sodium ion, the electrolyte test in the serum thing is once finished, simplify procedures.But its activity is little, easily to the (Cl in the electrolyte
-, Na
+, Ca
2+, K
+, H
+) disturb, thereby influence quality inspection.
The zinc metallic element is the needed by human body element, do not disturb the detection of other metal ion in the blood, its heparinate has multiple anticoagulation, as anti-deactivation serinase, anticoagulant proenzyme etc., do not produce haemolysis, three hospitals, Wuhan University's hospital clinical prove warp in Wuhan, and heparin zinc is preferable a kind of anticoagulant heparinate in the heparinate of being found at present.
The vacuum test tube that presets heparin zinc in test tube can replace existing different anticoagulant test tubes, can make things convenient for medical personnel's identification and operation, improves quality inspection.
The specific embodiment
A kind of method of production heparin zinc is carried out ion exchange with heparin sodium by the porous type cation exchange resin and is obtained heparin zinc; Concrete grammar is heparin sodium to be added water dissolve the back exchanger resin exchange of crossing fully, uses the distilled water eluting then, and cold drying gets required heparin zinc.Used heparin sodium molecular weight is about 15000,1,100,000 medical units; Used exchanger resin is acid type polystyrol cation exchange resin.
A kind of heparin zinc vacuum is contained blood vessel, presets heparin zinc in the vacuum blood vessel.The consumption that is preset in the heparin zinc in the standard vacuum test tube is 1-2mg.
Claims (4)
1, a kind of heparin zinc vacuum is contained blood vessel, it is characterized in that: preset heparin zinc in the vacuum blood vessel.
2, plant vacuum as claimed in claim 1 and contain blood vessel, it is characterized in that: the consumption that is preset in the heparin zinc in the standard vacuum test tube is 1-2mg.
3, a kind of method of production heparin zinc is characterized in that: heparin sodium is carried out ion exchange by the porous type cation exchange resin obtain heparin zinc; Concrete grammar is heparin sodium to be added water dissolve the back exchanger resin exchange of crossing fully, uses the distilled water eluting then, and cold drying gets required heparin zinc.
4, production method as claimed in claim 3 is characterized in that: used heparin sodium molecular weight is about 15000,1,100,000 medical units; Used exchanger resin is acid type polystyrol cation exchange resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031284000A CN1513418A (en) | 2003-07-29 | 2003-07-29 | Heparin zinc vacuum blood-holding tube, and technique for preparing heparin zinc |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031284000A CN1513418A (en) | 2003-07-29 | 2003-07-29 | Heparin zinc vacuum blood-holding tube, and technique for preparing heparin zinc |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1513418A true CN1513418A (en) | 2004-07-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031284000A Pending CN1513418A (en) | 2003-07-29 | 2003-07-29 | Heparin zinc vacuum blood-holding tube, and technique for preparing heparin zinc |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308351C (en) * | 2005-11-21 | 2007-04-04 | 武汉理工大学 | Exchange method of ionic equilibriom for heparin lithium |
| CN101890189A (en) * | 2009-05-22 | 2010-11-24 | 北京奥萨医药研究中心有限公司 | Anticoagulant tube with built-in heparin and preparation method thereof |
| CN109280097A (en) * | 2018-05-18 | 2019-01-29 | 山阳县恒桓生物科技有限公司 | A kind of anti-coagulant heparin production of chemicals technique |
| CN111909288A (en) * | 2020-08-13 | 2020-11-10 | 山东辰龙药业有限公司 | Refining method of heparin sodium |
| CN112354575A (en) * | 2020-11-18 | 2021-02-12 | 珠海市康利莱医疗器械有限公司 | Production device and process suitable for heparin anticoagulant tube |
-
2003
- 2003-07-29 CN CNA031284000A patent/CN1513418A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308351C (en) * | 2005-11-21 | 2007-04-04 | 武汉理工大学 | Exchange method of ionic equilibriom for heparin lithium |
| CN101890189A (en) * | 2009-05-22 | 2010-11-24 | 北京奥萨医药研究中心有限公司 | Anticoagulant tube with built-in heparin and preparation method thereof |
| CN109280097A (en) * | 2018-05-18 | 2019-01-29 | 山阳县恒桓生物科技有限公司 | A kind of anti-coagulant heparin production of chemicals technique |
| CN111909288A (en) * | 2020-08-13 | 2020-11-10 | 山东辰龙药业有限公司 | Refining method of heparin sodium |
| CN112354575A (en) * | 2020-11-18 | 2021-02-12 | 珠海市康利莱医疗器械有限公司 | Production device and process suitable for heparin anticoagulant tube |
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