CN114047107A - Diluent for blood cell analysis - Google Patents
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- CN114047107A CN114047107A CN202111241962.2A CN202111241962A CN114047107A CN 114047107 A CN114047107 A CN 114047107A CN 202111241962 A CN202111241962 A CN 202111241962A CN 114047107 A CN114047107 A CN 114047107A
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- 210000000601 blood cell Anatomy 0.000 title claims abstract description 85
- 239000003085 diluting agent Substances 0.000 title claims abstract description 72
- 238000004458 analytical method Methods 0.000 title claims abstract description 54
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 63
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 26
- RDEIXVOBVLKYNT-VQBXQJRRSA-N (2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(1-aminoethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(aminomethyl)oxan-2-yl]o Chemical compound OS(O)(=O)=O.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@@H](CN)O2)N)[C@@H](N)C[C@H]1N.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@H](O2)C(C)N)N)[C@@H](N)C[C@H]1N.O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N RDEIXVOBVLKYNT-VQBXQJRRSA-N 0.000 claims abstract description 17
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims abstract description 13
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004327 boric acid Substances 0.000 claims abstract description 13
- 239000011780 sodium chloride Substances 0.000 claims abstract description 13
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims abstract description 5
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims abstract description 5
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims abstract 4
- 229960002645 boric acid Drugs 0.000 claims description 3
- 235000010338 boric acid Nutrition 0.000 claims description 3
- 235000002639 sodium chloride Nutrition 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 11
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract description 10
- 239000003755 preservative agent Substances 0.000 abstract description 7
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 6
- 230000002335 preservative effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000003204 osmotic effect Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 31
- 210000003743 erythrocyte Anatomy 0.000 description 14
- 238000001514 detection method Methods 0.000 description 13
- 238000010790 dilution Methods 0.000 description 11
- 239000012895 dilution Substances 0.000 description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 210000000265 leukocyte Anatomy 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 102000001554 Hemoglobins Human genes 0.000 description 6
- 108010054147 Hemoglobins Proteins 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 210000003677 hemocyte Anatomy 0.000 description 5
- 229940000351 hemocyte Drugs 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000002421 anti-septic effect Effects 0.000 description 3
- 238000004820 blood count Methods 0.000 description 3
- 229960001484 edetic acid Drugs 0.000 description 3
- 238000005534 hematocrit Methods 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 229910017053 inorganic salt Inorganic materials 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000005536 corrosion prevention Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
-
- G01N2015/012—
-
- G01N2015/016—
-
- G01N2015/018—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G01N2015/1024—
Abstract
The invention relates to the field of blood cell analysis, in particular to a diluent for blood cell analysis. Disclosed is a diluent for blood cell analysis, which is characterized by comprising per liter: the content of anhydrous sodium sulfate is 2.0-5.0g, the content of sodium chloride is 5.0-9.0g, the content of disodium ethylene diamine tetraacetate is 0.20-0.35g, the content of boric acid is 2.0-3.5g, the content of gentamicin sulfate is 0.05-0.10g, and the content of formaldehyde is 0.3-0.8 mL. The diluted solution for blood cell analysis has the conductivity range of 15-20mS/cm, the pH range of 6.8-7.8 and the osmotic pressure range of 300-330 mOsm/kg. In the formula, gentamicin sulfate and formaldehyde replace the common antibacterial preservative sodium azide, so that the damage to related personnel and environment in the production and use processes is avoided, the proper ionic strength is provided, the stable environment is provided for counting blood cells, and the classification counting is facilitated.
Description
Technical Field
The invention relates to the field of blood cell analysis, in particular to a diluent for blood cell analysis.
Technical Field
In the field of medical clinical testing, the determination of blood cells is of great clinical significance for the diagnosis of the physical condition of a patient and for the diagnosis and subsequent treatment of diseases. In analyzing blood cells by a blood cell analyzer, it is necessary to dilute the blood cells with a diluent in order to analyze the cells. However, the existing diluent for blood cell analysis is generally a reagent matched with the original factory of the imported blood cell analyzer, and is expensive and inconvenient to transport; at present, although the effect of the diluent for analyzing the domestic blood cells is equivalent to that of an imported reagent, the price of the diluent is also higher; although some domestic reagents are cheap, the stability is poor, the accuracy of a test result cannot be guaranteed, and the machine may be damaged in an unexstimable manner. Meanwhile, most manufacturers still use sodium azide as an antibacterial preservative, which has certain damage to the environment and production personnel.
Disclosure of Invention
The embodiment of the invention provides a diluent for analyzing blood cells, which can solve the technical problems of high price, unstable performance and unsafe antibacterial preservative.
In order to solve the above technical problems, one technical solution adopted by the embodiments of the present invention is:
an embodiment of the present invention provides a diluent for blood cell analysis, including:
anhydrous sodium sulfate, sodium chloride, disodium ethylene diamine tetraacetate, boric acid, gentamicin sulfate and formaldehyde; wherein:
in each liter of the diluent for blood cell analysis, the content of anhydrous sodium sulfate is 2.0-5.0g, the content of sodium chloride is 5.0-9.0g, the content of disodium edetate is 0.20-0.35g, the content of boric acid is 2.0-3.5g, the content of gentamicin sulfate is 0.05-0.10g, and the content of formaldehyde is 0.3-0.8 mL.
Optionally, the conductivity of the dilution for blood cell analysis ranges from 15 to 20 mS/cm.
Optionally, the pH of the dilution for blood cell analysis ranges from 6.8 to 7.8.
Optionally, the osmolality of the diluent for blood cell analysis is in the range of 300-330 mOsm/kg.
Preferably, the anhydrous sodium sulfate content is 3.0 g/L.
Preferably, the sodium chloride content is 4.5 g/L.
Preferably, the boric acid content is 3.0 g/L.
Preferably, the content of the disodium ethylene diamine tetraacetate is 0.30 g/L.
Preferably, the content of the gentamicin sulfate is 0.08 g/L.
Preferably, the formaldehyde content is 0.5 mL/L.
More preferably, the pH of the diluent for blood cell analysis is 7.4.
More preferably, the osmolality of the diluent for blood cell analysis is 320 mOsm/kg.
More preferably, the conductivity of the dilution for blood cell analysis is 16.50 mS/cm.
Compared with the prior art, the embodiment of the invention has the beneficial effects that: the diluent for analyzing the blood cells provided by the embodiment of the invention has the advantages of low raw material cost, simple preparation process, no need of special operation, and only need of general physical dissolution, volume determination and uniform mixing; in the formula, gentamicin sulfate and formaldehyde replace the common antibacterial preservative sodium azide, so that the preparation method is safe, the damage to related personnel and environment in the production and use processes is avoided, the used formaldehyde has an antiseptic effect and also has the effect of fixing blood cells, the shape and the structure of the blood cells can be kept complete to a certain extent, and the effect of resisting mechanical damage of the blood cells can be enhanced; the diluent for blood cell analysis provided by the embodiment of the invention provides the most preferable conductivity value, provides proper ionic strength, provides a stable environment for counting blood cells, and is more beneficial to classified counting. When the diluent for analyzing the blood cells is used for detecting a whole blood sample, the test result reproducibility is good, and the accuracy is high.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to specific embodiments below. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The embodiment of the invention provides a diluent for a blood cell analyzer. The diluent for blood cell analysis includes: anhydrous sodium sulfate, sodium chloride, disodium ethylene diamine tetraacetate, boric acid, gentamicin sulfate and formaldehyde.
Wherein, in each liter of the diluent for hemocyte analysis, the content of anhydrous sodium sulfate is 2.0 to 5.0g, the content of sodium chloride is 5.0 to 9.0g, the content of disodium edetate is 0.20 to 0.35g, the content of boric acid is 2.0 to 3.5g, the content of gentamicin sulfate is 0.05 to 0.10g, and the content of formaldehyde is 0.3 to 0.8 mL.
The procedure for preparing the above-mentioned diluent for blood cell analysis is shown in FIG. 1:
100. firstly, adding the components into a beaker, and dissolving the components by using a certain amount of pure water;
101. secondly, the solution was transferred from the beaker to a volumetric flask with a glass rod;
102. then using a dropper to fix the volume to 1 liter by using pure water;
103. after the volume is fixed, the mixture is shaken up.
The anhydrous sodium sulfate and the sodium chloride are inorganic salts, and the diluted inorganic salt solution and other components jointly provide the blood cells with the conditions similar to the human inorganic salt environment, so that the blood cells are detected in a proper pH range, a proper osmotic pressure range and a proper conductivity range; boric acid is used as a buffering agent, and has a buffering effect, and environmental pollution is avoided because phosphorus is not contained; disodium edetate is an anticoagulant that can act to clot tissue blood cells; gentamicin sulfate and formaldehyde are preservatives, play a role in bacteriostasis and corrosion prevention, wherein the formaldehyde also has a function of fixing blood cells, can keep the shape and the structure of the blood cells complete to a certain extent, can also increase the effect of resisting mechanical damage of the blood cells, and ensures the accuracy of detection.
In some embodiments, the osmolality of the dilution for blood cell analysis is 320 mOsm/kg.
In some embodiments, it is surprisingly found that the conductivity of the diluted solution for blood cell analysis is an optimal conductivity value at 16.50mS/cm, which can provide a suitable ionic strength, provide a stable environment for blood cell counting, and is more beneficial for classification and counting.
In the embodiment of the invention, gentamicin sulfate and formaldehyde are used for replacing a common antibacterial preservative sodium azide, so that the preparation method is safe, the damage to related personnel and environment in the production and use processes is avoided, the used formaldehyde has an antiseptic effect and also has the effect of fixing blood cells, the shape and the structure of the blood cells can be kept complete to a certain extent, and the effect of resisting mechanical damage of the blood cells can be enhanced; when the diluent for analyzing the blood cells is used for detecting a whole blood sample, the test result reproducibility is good, and the accuracy is high.
Hereinafter, a plurality of examples are provided to explain in detail the detection effect and accuracy of the diluent for blood cell analysis according to the embodiment of the present invention.
Example 1: a hemocyte analysis diluent 1:
in the diluent for blood cell analysis provided in this embodiment, each liter of the diluent includes: 3.00g of anhydrous sodium sulfate, 4.50g of sodium chloride, 3.00g of boric acid, 0.30g of ethylene diamine tetraacetic acid, 0.08g of gentamicin sulfate and 0.50mL of formaldehyde; the specific configuration method comprises the following steps: the components are dissolved by pure water, and then the pure water is used for fixing the volume to 1 liter. The pH of this dilution was 7.4.
Example 2: a hemocyte analysis diluent 2:
in the diluent for blood cell analysis provided in this embodiment, each liter of the diluent includes: 3.50g of anhydrous sodium sulfate, 5.00g of sodium chloride, 3.00g of boric acid, 0.30g of ethylene diamine tetraacetic acid, 0.08g of gentamicin sulfate and 0.50mL of formaldehyde; the specific configuration method comprises the following steps: the components are dissolved by pure water, and then the pure water is used for fixing the volume to 1 liter. The pH of this dilution was 7.4.
Example 3: a hemocyte analysis diluent (3):
in the diluent for blood cell analysis provided in this embodiment, each liter of the diluent includes: 3.50g of anhydrous sodium sulfate, 5.00g of sodium chloride, 3.00g of boric acid, 0.25g of ethylene diamine tetraacetic acid, 0.08g of gentamicin sulfate and 0.50mL of formaldehyde; the specific configuration method comprises the following steps: the components are dissolved by pure water, and then the pure water is used for fixing the volume to 1 liter. The pH of this dilution was 7.4.
Example 4: background count experiment:
after the blood cell analyzer is started, the machine can automatically clean the internal pipeline, then automatically measure the background and generate a technical interface; after a technical interface appears, the machine displays the automatically measured background value on a screen, and the background result requires that: WBC is less than or equal to 0.3, RBC is less than or equal to 0.03, HGB is less than or equal to 1, and PLT is less than or equal to 10; if the background does not meet the requirements of the instrument, the instrument will prompt "background exception" to perform a cleaning or maintenance procedure.
The dilutions of the samples of examples 1-3 of the present invention were used to perform background counting experiments on a blood cell analyzer. Specifically, three hematology analyzers, namely Merrill BC-5000, Merrill DF-53 and Jinrui KT-6610 are respectively used, according to the specification of the hematology analyzer, original factory diluent in the hematology analyzer is emptied, the diluent in the embodiment 1-3 of the invention is respectively added, other reagents are original factory reagents, and after the diluent is replaced, the diluent in the embodiment 1-3 of the invention is used as a sample to carry out a background counting experiment.
Respectively accessing the diluent of the embodiment 1-3 of the invention by using a Merrill BC-5000 blood cell analyzer, taking the diluent of the embodiment 1-3 of the invention as a sample, carrying out background counting for 5 times, then calculating an average value, and obtaining a detection result shown in a table 1;
respectively accessing the diluent of the embodiment 1-3 of the invention by using an imperial DF-53 blood cell analyzer, taking the diluent of the embodiment 1-3 of the invention as a sample, carrying out background counting for 5 times, then calculating an average value, and obtaining a detection result shown in a table 2;
the diluted solutions of the samples of.
Wherein: WBC means white blood cells, RBC means red blood cells, HGB means hemoglobin, HCT means hematocrit (also known as hematocrit or hematocrit), PLT means platelets.
TABLE 1 background count test results (Mirui BC-5000)
TABLE 2 background count test results (Dimai DF-53)
TABLE 3 background count test results (jin Rui KT-6610)
As can be seen from tables 1-3, in three different hematology analyzers, Merrill BC-5000, Dimai DF-53 and Jinrui KT-6610, the background values measured by using any one of the diluents provided in examples 1-3 of the present invention as the diluent all meet the requirements of the analyzer.
Example 5: repeatability of test results:
the diluent for cell analysis provided by the embodiments 1 to 3 of the present invention has pH values, osmotic pressures and conductivity values similar to the environment of blood cells in human body, provides a stable environment for counting blood cells, and is more favorable for classification counting; testing a whole blood sample by using the diluent for cell analysis on three different hematology analyzers, namely a Merrill BC-5000, a Dimaili DF-53 and a Jinrui KT-6610; the same whole blood sample was tested in duplicate 10 times on different hematology analyzers with different dilutions for cell analysis.
The results of the test on the hematology analyzer Merrill BC-5000 are presented in Table 4;
the results of the tests on the hematology analyzer, camei DF-53, are presented in table 5;
the results of the test on the hematology analyzer jinrikt-6610 are presented in table 6;
wherein: WBC means white blood cells, RBC means red blood cells, HGB means hemoglobin, MCV means mean red blood cell volume, PLT means platelets.
TABLE 4 repeatability tests results (Mirui BC-5000)
TABLE 5 repeatability test results (Dimai DF-53)
TABLE 6 repeatability tests results (jin rui KT-6610)
As shown in tables 4 to 6, in three different hematology analyzers, Meyer BC-5000, Dimai DF-53 and Jinrui KT-6610, the test results of the dilution provided in examples 1 to 3 of the present invention have excellent reproducibility when the whole blood sample is tested.
Example 6: accuracy comparison experiment:
as shown in Table 7, 3 examples of healthy fresh whole blood samples were each measured by a hemocytometer Meyer BC-5000 using the diluent for cell analysis provided in examples 1 to 3 of the present invention, and the measurement results were compared with the measurement results of the microscopic examination for accuracy.
TABLE 7 accuracy test results (Mirui BC-5000)
As can be seen from the above-mentioned test results, the total white blood cell count, total red blood cell count, hemoglobin, average red blood cell volume, and platelet count obtained by testing the diluent for blood cell analysis provided in the embodiment of the present invention on the micheli BC-5000 are not significantly different from the test results obtained by microscopy; therefore, the detection result of the diluent for blood cell analysis provided by the embodiment of the invention on Merrill BC-5000 has higher accuracy.
As shown in table 8, 3 healthy fresh whole blood samples were measured by a hematology analyzer, camei DF-53, using the dilutions for cell analysis provided in embodiments 1 to 3 of the present invention, respectively, and the measurement results were compared with the measurement results of the microscopy for accuracy evaluation;
TABLE 8 accuracy test results (Dimai DF-53)
As can be seen from the above-mentioned test results, the test results of the total number of leukocytes, the total number of erythrocytes, hemoglobin, the average volume of erythrocytes, and the number of platelets obtained by the test on the deltoid DF-53 using the diluent for analyzing blood cells provided in the embodiments of the present invention are not significantly different from the test results obtained by the microscopy; therefore, the detection result of the diluent for blood cell analysis on the Dimai DF-53 provided by the embodiment of the invention has higher accuracy.
As shown in table 9, 3 examples of healthy fresh whole blood samples were tested by a hemocyte analyzer brochure KT-6610 using the diluent for cell analysis provided in embodiments 1 to 3 of the present invention, and the test results were compared with the test results of the microscopic examination for accuracy evaluation;
TABLE 9 accuracy test results (jin rui KT-6610)
According to the detection results in the table, the detection results of the total number of white blood cells, the total number of red blood cells, hemoglobin, the average red blood cell volume and the number of platelets obtained by testing the diluent for blood cell analysis provided by the embodiment of the invention on the brocade KT-6610 are not obviously different from the detection results of the microscopic examination method; therefore, the detection result of the diluent for analyzing the blood cells provided by the embodiment of the invention on the brocade KT-6610 has higher accuracy.
According to the comparative analysis of the detection results in tables 7-9, the total number of white blood cells, total number of red blood cells, hemoglobin, average red blood cell volume and platelet number obtained by testing on Meyer BC-5000, Dimei DF-53 and Jinrui KT-6610 by using the diluent for blood cell analysis provided by the embodiment of the present invention have no significant difference from the detection results of the microscopic examination method; therefore, the detection results of the diluent for blood cell analysis provided by the embodiment of the invention on Merrill BC-5000, Dimaill DF-53 and Jinrui KT-6610 have higher accuracy.
In summary, compared with the prior art, the diluent for analyzing blood cells provided by the embodiment of the invention has the advantages of low raw material cost, simple preparation process, no need of special operation, and only need of general physical dissolution, volume determination and uniform mixing; in the formula, gentamicin sulfate and formaldehyde replace the common antibacterial preservative sodium azide, so that the preparation method is safe, the damage to related personnel and environment in the production and use processes is avoided, the used formaldehyde has an antiseptic effect and also has the effect of fixing blood cells, the shape and the structure of the blood cells can be kept complete to a certain extent, and the effect of resisting mechanical damage of the blood cells can be enhanced; the diluent for blood cell analysis provided by the embodiment of the invention provides the most preferable conductivity value, provides proper ionic strength, provides a stable environment for counting blood cells, and is more beneficial to classified counting. When the diluent for analyzing the blood cells is used for detecting a whole blood sample, the test result reproducibility is good, and the accuracy is high.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A diluent for blood cell analysis, comprising:
anhydrous sodium sulfate, sodium chloride, disodium ethylene diamine tetraacetate, boric acid, gentamicin sulfate and formaldehyde; wherein:
in each liter of the diluent for blood cell analysis, the content of anhydrous sodium sulfate is 2.0-5.0g, the content of sodium chloride is 5.0-9.0g, the content of disodium edetate is 0.20-0.35g, the content of boric acid is 2.0-3.5g, the content of gentamicin sulfate is 0.05-0.10g, and the content of formaldehyde is 0.3-0.8 mL.
2. The hemocyte-analyzing diluent according to claim 1, wherein the conductivity of the hemocyte-analyzer diluent is in the range of 15 to 20 mS/cm.
3. The diluent for blood cell analysis according to claim 1, wherein the pH of the diluent for blood cell analysis is in the range of 6.8 to 7.8.
4. The diluent for blood cell analysis according to claim 1, wherein the osmolality of the diluent for blood cell analyzer is in the range of 300-330 mOsm/kg.
5. The diluent for blood cell analysis according to any one of claims 1 to 4, wherein the conductivity of the diluent for blood cell analyzer is 16.50 mS/cm.
6. The diluted solution for blood cell analysis according to any one of claims 1 to 4, wherein the concentration of the anhydrous sodium sulfate is 3.0g/L, and the concentration of the sodium chloride is 4.5 g/L.
7. The diluent for blood cell analysis according to any one of claims 1 to 4, wherein the boric acid has a concentration of 3.0 g/L.
8. The diluent for blood cell analysis according to any one of claims 1 to 4, wherein the concentration of disodium edetate is 0.30 g/L.
9. The diluent for blood cell analysis according to any one of claims 1 to 4, wherein the concentration of gentamicin sulfate is 0.08 g/L.
10. The diluent for blood cell analysis according to any one of claims 1 to 4, wherein the concentration of formaldehyde is 0.5 mL/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115494244A (en) * | 2022-11-21 | 2022-12-20 | 保定佳瑞源生物芯片有限公司 | Acridinium ester antibody marker diluent of cancer antigen CA724 and application thereof |
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