CN1509729A - Yanning adjuvant - Google Patents

Abstract

A Chinese medicine 'Yanningdadang' for treating acute or chronic pharyngitis and diphtheria is disclosed. Its advantages are high curative effect and low cost.

Description

Yanning adjuvant

Technical field: Chinese patent medicine

Source of data: the Yanning adjuvant prescription improves one group of Chinese medicine proved recipe forming for Mr. Tang Xulei adopts " electuary " for oral administration to sum up in conjunction with the experience of local " powder " treatment, by theory of Chinese medical science according to treatment pharyngitis from generation to generation handed down from one's ancestors.

Goal of the invention: improve classic side's medicine, carry forward national medicine, promote the well-being of mankind.

One, preface:

Pharyngeal is one of the vitals of human body more complicated, according to domestic statistics, pharyngeal morbidity is more than 30%, pharyngeal pharyngitis and tonsillitis are the higher diseases of sickness rate, account for second of whole body sickness rate, because China's economy is still located the early stage of development, present many disease of pharynx can't in time obtain medical treatment, still have various people decocted by the misery of disease of pharynx, so the pharyngitis medicine of the more high-efficiency low-toxicities of exploitation is fit to the needs of Economic Development and people ' s health.

Two, prescription:

1, the Yanning adjuvant electuary is formed:

Radix Sophorae Tonkinensis 8g Radix Platycodonis 10g Bombyx Batryticatus 8g

Cortex Moutan 9g Rhizoma Belamcandae 7g Herba Schizonepetae 9g

Radix Saposhnikoviae 8g Flos Chrysanthemi 8g Herba Menthae 4g

Radix Scrophulariae 12g Radix Rehmanniae 12g Fructus Forsythiae 10g

Radix Glycyrrhizae 7g Bulbus Fritillariae Thunbergii 8g

2, Yanning adjuvant powder is formed:

Borneolum Syntheticum 3.5g Borax 4.0g Concha Margaritifera (forging) 7g

Mentholum 4.0g Indigo Naturalis 1.5g Gypsum Fibrosum Preparatum 7g

Three, function:

Heat-clearing and toxic substances removing, dispelling wind and eliminating swelling, analgesic antiphlogistic, act as expectorant

Four, cure mainly:

Acute/chronic pharyngitis, tonsillitis, diphtheria

Five, the side separates:

1, Yanning adjuvant electuary side separates:

Acute pharyngitis is many because of due to the strongly fragrant fire-transformation of affection due to external wind and heat, the invasion and attack in addition of evil drug-pedlar's part of the body cavity above the diaphragm housing the heart and lungs, and interior-heat Yong Sheng, expectorant heat are copied mutually.So with Radix Sophorae Tonkinensis and Radix Platycodonis is monarch drug, heat-clearing and toxic substances removing, the lung qi dispersing sore-throat relieving, detumescence is eliminated the phlegm, the evacuation of pus cough-relieving.

Heat is trembled with fear it, in the event side with the relatively large Radix Rehmanniae, Radix Scrophulariae is with the auxiliary Radix Sophorae Tonkinensis of the product of detoxifcation, for example: Radix Platycodonis clearing throat, Cortex Moutan are clearing away heat and cooling blood, promoting blood circulation to remove blood stasis, again with Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae, Flos Chrysanthemi disperse wind heat, the detumescence of dispeling the wind, relieving the exterior syndrome by diaphoresis, the dehumidifying spasmolytic, more than the common ministerial drug of forming of seven flavors.

The intenseness of heat consumption is hindered seminal fluid, throat is foster prior to moistening, so with Radix Scrophulariae, the Radix Rehmanniae, assistant with principal agent Radix Platycodonis, replenishing YIN and removing heat, clearly disappear floating fire, eliminate heat-phlegm, more than four medicines form adjuvant drug jointly, the pathogenic fire purging of reuse Radix Glycyrrhizae is reduced phlegm, to concoct all medicines be messenger drug, monarch, minister, help, make comprehensive each medicine to share, treating both the principal and secondary aspects of a disease, and the exterior and the interior is laid equal stress on, heat-clearing and toxic substances removing, the cold and accent of heat.

2, Yanning adjuvant powder side separates:

Even diseases such as red, swollen, pain is festered, suppuration, hoarseness are back the common sympton of laryngopathy, how go up due to the harmony throat by affection due to external wind and heat and accumulated heat in the lung and stomach are rich mutually, punching is arduous in the side is slightly cold, and its property fragrance is walked to scurry, it is hot and suffocating to loose, controlling various skin infection and laryngopharynx swelling and pain is our monarch drug, Indigo Naturalis, Borax heat-clearing and toxic substances removing, the principal drug assistance eliminating fire and detoxication is ministerial drug altogether, Gypsum Fibrosum powder (calcined), Mentholum, Concha Margaritifera pain relieving, ending flesh is adjuvant altogether.

Six, preparation technology:

1, Yanning adjuvant electuary preparation technology

(1), be that molten coal extracts mainly with water according to electuary prescription medicine physico-chemical analysis result.

(2) medical material that Herba Schizonepetae, Radix Saposhnikoviae, Fructus Forsythiae, Herba Menthae etc. are mainly contained the volatile oil composition then extracts volatile oil earlier, medicinal residues reuse water extraction.

When (3) decocting, at first add water with 7～8 times of amounts of medical material, for the second time amount of water is 5～6 times of medical material.

(4) contain volume mucus in the decocting liquid, very easily moisture absorption of glucide, mould losing, be convenient to take and preserve for making preparation, adding when equivalent ethanol makes contamination precipitation add ethanol after event is concentrated into 1: 1 with decocting liquid answers the limit edged to stir, after adding ethanol, should place at least 24 hours, otherwise can produce inclusion, cause the active ingredient loss; To the medical material of high sugar content such as the Radix Rehmanniae, Radix Scrophulariae, Radix Glycyrrhizae etc., its decocting liquid should add triplication ethanol after concentrating, and can remove most of sugar part, reaches and reduces the purpose that listens to reason.

(5),, determine that the extraction process flow process is as follows: (seeing Figure of description) in conjunction with the calibrating of animal pharmacodynamics through multiple extraction test

Three batches of extractum recovery rates are:

13.18% 12.52% 13.70% average 13.13%

[character] this product content is tan granule; It is sweet to distinguish the flavor of, little hardship.

[discriminating] (1) term system is checked the alkaloid in the Radix Sophorae Tonkinensis.

(2) term system is differentiated Herba Menthae.

(3) a discriminating Cortex Moutan.

(4) a discriminating Radix Glycyrrhizae.

Intend adopting medical material out of the ordinary to compare the product medical material to other medical materials, explore its discrimination method.

[inspection] pressed under 10 pages of electuary items of appendix of distiller and checked, should reach every requirement of regulation.

[assay] mainly measures the saponin content of Radix Platycodonis.

[function with cure mainly] heat-clearing and toxic substances removing, dispelling wind and eliminating swelling, anti-inflammatory analgetic, act as expectorant, cure mainly acute pharyngolaryngitis, tonsillitis etc.

[usage and consumption] boiled water is taken after mixing it with water, a 15g, 3 times on the one.

[specification] In Aluminium Foil Packing, every bag heavy 15g.

[storage] sealing is put shady and cool place and is preserved.

List of references

1, Pharmacopoeia of the People's Republic of China nineteen ninety version is one one

2, the new medical college in " Chinese medicine voluminous dictionary " Jiangsu compiles 1986

Yanning adjuvant electuary clinical preparation quality standard

The Yanning adjuvant electuary

Shenhua?Yanyanping?chongji

[prescription]

Radix Sophorae Tonkinensis 8g Radix Platycodonis 10g Bombyx Batryticatus 8g

Pellet degree 9g Rhizoma Belamcandae 7g Herba Schizonepetae 9g

Radix Saposhnikoviae 8g Flos Chrysanthemi 8g Herba Menthae 4g

Radix Scrophulariae 12g Radix Rehmanniae 12g Fructus Forsythiae 10g

Radix Glycyrrhizae 7g Bulbus Fritillariae Thunbergii 8g

[method for making] above 14 flavors are got Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae distillation sampling hair oil, and the aqueous solution after the distillation is collected in addition; Medicinal residues and Cortex Moutan, Radix Platycodonis, Radix Sophorae Tonkinensis, Flos Chrysanthemi seven flavors decoct with water secondary, 1 hour for the first time, 40 minutes for the second time, collecting decoction, filter, filtrate and above-mentioned aqueous solution merge, and are concentrated into every ml and are equivalent to the 1g medical material, are chilled to room temperature, add equal-volume ethanol and make precipitation, left standstill 24 hours, and got the supernatant concentrating under reduced pressure, be decocting liquid I.

Get the Radix Rehmanniae, Radix Scrophulariae, Radix Glycyrrhizae and decoct with water secondary, about at every turn half an hour, collecting decoction filters, and filtrate is the same to be concentrated into every ml and to be equivalent to the 1g medical material, is chilled to room temperature, adds 3 times of ethanol and makes precipitation, leaves standstill, and getting the supernatant concentrating under reduced pressure is decocting liquid II.

Collecting decoction I and decocting liquid II are concentrated into the clear paste of relative density 1.35～1.38,1 part of qinghuo reagent, and 0.04 part of sweeting agent, 2 parts in dextrin and 80% ethanol are made granule in right amount, and drying adds volatile oil and Mentholums such as above-mentioned Herba Schizonepetae, mixing, promptly.

[character] this product is a brown granular; It is sweet to distinguish the flavor of, little hardship.

[discriminating] (1) is differentiated under the item by pharmacopeia " Radix Sophorae Tonkinensis " and is carried out the alkaloid inspection.

(2) get this product 8g, be ground into fine powder, add petroleum ether (60～90 ℃) 40ml, close plug, jolting number minute was placed 30 minutes, filtered, and filtrate is as need testing solution, and other gets the Mentholum reference substance solution.By thin layer chromatography (57 pages of [hereinafter to be referred as pharmacopeia] appendix of nineteen ninety version pharmacopeia) test, get above-mentioned test liquid 20～40 μ l, reference substance solution 10 μ l, put respectively on same silica gel g thin-layer plate, with benzene-ethyl acetate (119: 1) is developing solvent, expansion, taking-up, airing, spray are with the mixed liquor of 2% vanillin sulphuric acid ethanol (2: 8), about 5～10 minutes of 100 ℃ of bakings.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.

(3) get this product 5g, the 40ml that adds diethyl ether, close plug, jolting 40 minutes is filtered, and filtrate volatilizes, and residue adds acetone 2ml makes dissolving, as need testing solution; Other gets the paeonol reference substance and adds acetone and make the solution that 1ml contains 5mg, in contrast product solution.Test according to thin layer chromatography (57 pages of pharmacopeia appendix), drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with epoxy hexane-ethyl acetate (3: 1), launch, take out, dry, spray is with the acid 5% ferric chloride alcoholic solution of hydrochloric acid, it is clear that hot blast blows to the speckle colour developing, in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show identical blue brown speckle.

(4) get this product 15g, the 80ml that adds diethyl ether put in the water-bath reflux 1 hour, filter, filtrate evaporate to dryness, residue add water 40ml makes dissolving, aqueous solution n-butanol extraction three times, each 20ml, merge n-butyl alcohol liquid, wash with water three times, put evaporate to dryness in the water-bath, residue adds methanol 5ml makes dissolving, as need testing solution; Other extracting liquorice acid ammonium reference substance adds methanol and makes the solution that 1ml contains 2mg, in contrast product solution.Test according to thin layer chromatography (57 pages of pharmacopeia appendix), drawing each 5 μ l of above-mentioned responsibility kind solution, put respectively on the silica gel g thin-layer plate of same usefulness 1% sodium hydroxide solution preparation, is developing solvent with ethyl acetate-formic acid-glacial acetic acid-water (30: 2: 4), launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, dry by the fire to clear the putting under the uviol lamp (365nm) of speckle colour developing at 105 ℃ and to inspect, in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show identical orange-yellow fluorescence speckle.

[inspection] should meet every regulation relevant under the electuary item (10 pages of appendix of pharmacopeia).

[assay] gets this product 25g, and precision steelyard is fixed, puts in the cable-styled extraction, adds methanol 75ml, and merceration adds methanol 25ml after 15 hours again, and reflux 6 hours was placed liquid, filters.Filtrate is put and is concentrated into 20～25ml in the water-bath, puts coldly, adds ethanol 50ml, jolting, be placed to clear and bright, abandoning supernatant, the precipitation gradation adds methanol (20,10,5ml) heating makes dissolving, puts coldly, filters, merge methanol solution, put and be concentrated into 20～25ml in the water-bath, put coldly, 50ml adds diethyl ether, jolting, the same processing.Merge methanol solution, in the evaporating dish of constant weight, evaporate to dryness in water-bath is dried to constant weight at 105 ℃, calculates the percentage composition of total saponin from platycodon grandiflorum, promptly.

[function with cure mainly] heat-clearing and toxic substances removing, dispelling wind and eliminating swelling, analgesic antiphlogistic, act as expectorant, cure mainly acute pharyngolaryngitis, tonsillitis and flu etc.

[usage and consumption] boiled water is taken after mixing it with water, a 15g, three times on the one.

[specification] every bag heavy 15g

[storage] sealing

" Yanning adjuvant powder " clinical preparation quality standard and draft explanation

1, major ingredient: Borax, Borneolum Syntheticum, Concha Margaritifera (forging), Mentholum, Indigo Naturalis, Gypsum Fibrosum powder (calcined)

2, deal: in " powder " prescription ratio

3, method for making: earlier Concha Margaritifera, Gypsum Fibrosum are forged the back and become the end, again Borax, Borneolum Syntheticum, Mentholum, Indigo Naturalis are total to the porphyrize powder, sieve, collect weighing, bottling by No. eight sieves of national standard.

4, character: this product is the Lycoperdon polymorphum Vitt powder, and it is refrigerant to distinguish the flavor of, little hardship.

5, differentiate:

(1) carries out under differentiating by pharmacopeia " Borneolum Syntheticum " (nineteen ninety version one one 121 pages)

(2) carry out under differentiating by pharmacopeia " Borax " (nineteen ninety version two ones 776 pages)

6, check: should meet the every regulation under the pharmacopeia powder item

7, assay: see two ones 776～777 pages of pharmacopeia, the content assaying method of " Borax ".

8, function and curing mainly: heat-clearing and toxic substances removing, anti-inflammatory analgetic, the granulation promoting of reducing phlegm cures mainly acute pharyngitis, tonsillitis (membranous pharyngitis, throat moth, sore throat).

9, usage and consumption: spray into bottleneck throat, a 50mg, three times on the one.

10, specification: every bottle heavy 1g

11, storage: sealing.

Yanning adjuvant powder clinical preparation quality standard

Yanning adjuvant powder

Shenhua?Yanyan?San

[prescription] Borneolum Syntheticum 3.5g Borax 4.0g Concha Margaritifera (forging) 7g Mentholum 4g Indigo Naturalis 1.5g

Gypsum Fibrosum Preparatum 7g

[method for making] earlier with Concha Margaritifera, Gypsum Fibrosum is forged the back and is become the end, again Borax, Borneolum Syntheticum, Mentholum, Indigo Naturalis is total to the porphyrize powder, collects the weighing bottling by No. eight sieves of national standard.

[character] this product is the Lycoperdon polymorphum Vitt powder; It is refrigerant to distinguish the flavor of, little hardship.

This product 10mg is got in [discriminating] (1), adds the ethanol number and droplet makes dissolving, adds new system 1% vanillin sulfuric acid solution 1-2 and drips, and promptly shows purple.

(2) get this product 3g, add nitric acid 10ml, the gas of rufous promptly takes place, treat that gas stops after, add water 20ml, jolting filters, and after filtering residue washes, has Camphora smelly.

(3) get this product 100mg and be dissolved in water, aqueous solution shows the identification of sodium salt (39 pages of appendix) and borate (39 pages of appendix).

[inspection] should conform to the relevant every regulation (10 pages of appendix) of powder of one nine nine zero year pharmacopeia.

Get this pinkish red 0.4g, precision weighing, add water 25ml dissolving after, add 1 of 0.05 methyl orange solution, to orange red, boiled 2 minutes with hydrochlorate (0.1mol/L) titration, cooling, be yellow as solution, continue titration to solution and be orange red, add neutral glycerine 80ml, add 1 of water 20ml and phenolphthalein indication solution, to pink, 8 of 80ml and instructions phenolphthalein solutions are equivalent to the Na of 9.534mg with NaOH (0.1mol/L) with NaOH liquid (0.1mol/L) titration ₂B ₄O ₇10H ₂O.

[function with cure mainly] heat-clearing and toxic substances removing, the putrefaction removing detumescence cures mainly acute pharyngolaryngitis, tonsillitis.

[usage and consumption] local usefulness is blown at affected area, a 50mg, two to three times on the one.

[specification] every bottle of 1g.

[storage] sealing.

The acute toxicity test data of " Yanning adjuvant electuary "

1, material

1.1 be subjected to the reagent thing: the prescription of " Yanning adjuvant electuary " provides for Chinese herbal medicine technical research institute of Chongqing City Shenhua.Its crude drug extracts, and being processed into electuary is provided by Chongqing, Traditional Chinese Medicine Research Institute, Sichuan Province Noah medical sci-tech development company, during test with electuary or semi-finished product extracting solution:

(1) " electuary " is " Yanning adjuvant electuary " (it is 2g that the 1g electuary is equivalent to the crude drug amount), and a certain amount of electuary is put into the suspension that mortar porphyrize adding distil water is prepared becomes Cmax, and every 1ml suspension is equivalent to crude drug 5g.

(2) " extracting solution " is this electuary semi-finished product that do not add adjuvant, and it is 2.0g that the every ml of this liquid is equivalent to the crude drug amount.

This test measurement is all to contain crude drug g/kg or g/ml.

1.2 laboratory animal: Kunming and outbreeding system white mice, body weight 20 ± 2g healthy person, male and female half and half are provided by Animal Experimental Study chamber, Traditional Chinese Medicine Research Institute, Sichuan Province, meet primary standard (No. 32, the moving pipe in river).

2, experimental technique and result

According to the rules of chmice acute limit experiment [1-2] are carried out, get 40 body weight 20 ± 2g mices, male and female half and half are divided into two groups, and first group is " electuary " group, and second group is " extracting solution " group; Two groups all with Cmax and maximum volume 1ml/20g Mus jar stomach (1g) once, observes animal dead and other toxic reaction situations then, observes altogether seven days.Its result does not see a dead mouse, does not see other toxic reaction yet, for example " appetite ", weight loss, and mobility reduces, and defecation is undesired etc., sees table 1 for details.

The acute toxicity test of table 1 Yanning adjuvant electuary (X ± SD)

The dead survival of medicine sample dose volume Mus number number body weight (g)

Approach

(2g/ml) before and after the g/kg ml/20g (only) (only) (only)

Electuary 100 1.0 1g 20 0 20 20.5 ± 1.2 23.6 ± 1.6

Extract 100 1.0 1g 20 0 20 20.3 ± 1.1 23.2 ± 1.4

Table 1 result shows the filling stomach LD of Yanning adjuvant electuary and extract ₅₀All can not obtain.

3, evaluation of result

It is 128g that " Yanning adjuvant electuary " former prescription contains the crude drug amount altogether, and promptly everyone dosage every day is 2g/kg (by adult 60kg).This experiment reaches 100g/kg for the maximal dose of mouse stomach, is 50 times of adult's dosage, does not see the overt toxicity reaction, so think that the clinical dosage that former prescription is drafted is a safety non-toxic.

4, list of references

(1) Ministry of Health of the People's Republic of China develops and publishes: one nine nine two years JIUYUE of provisions for new drugs approval " about the revision and the supplementary provisions of Chinese medicine part " rose on the 1st implements P19～21.

(2) Xu Shuyun, Bian are as, Chen Xiu chief editor: the pharmacological experimental methodology second edition.The People's Health Publisher, 1990

The Pharmacodynamic test of active extract data and the documents and materials of " Yanning adjuvant electuary "

---the preliminary experiment of analgesia, antiinflammatory, refrigeration function

1, experiment material

1.1 be subjected to the reagent thing: the prescription of " Yanning adjuvant electuary " provides for Chinese herbal medicine technical research institute of Chongqing City Shenhua.Its crude drug is also provided by this institute, and crude drug extracts, and being processed into " electuary " is provided by Chongqing, Traditional Chinese Medicine Research Institute, Sichuan Province Noah medicine bioengineering scientific and technological development company.During pharmacodynamics test electuary is made into drug level 1g/ml (in the symphysis dose) with distilled water.

1.2 positive control drug:

Rotundine Tablets (tetrahydropalmatine): the 30mg/ sheet, produce in pharmaceutical factory, Dongting Lake, Hunan, lot number: 900818

Etidocine tablet (indomethacin): the 25mg/ sheet, Shi Qi pharmaceutical factory in Guangdong produces, lot number: 890748

Injection of Radix Bupleuri: 2ml/ props up, and contains crude drug 2g, and Pharmaceutical Factory of Luzhou Medical College produces, lot number: 910418

Cause the pain reagent: 0.7% acetic acid, get 0.7ml glacial acetic acid (A.R) adding distil water to 100ml.

Cause scorching reagent: get carrageenin (biological preparation) 1g and add normal saline 100ml and be made into 1% solution, 4 ℃ of refrigerators place spend the night standby.

The pyrogenicity vaccine: typhoid fever paratyphoid fever, second triple vaccine, 5mg/5ml/ props up, and Chengdu Biological Products Inst., Ministry of Public Health produces, lot number: 921002.

1.4 laboratory animal

Kunming kind outbreeding system white mice, age in 5-6 week, body weight 20 ± 2g, male and female are usefulness all, tests first three day dislocation laboratory, freely ingests by (standard particle feedstuff) and drink water by (tap water), tests to belong to homogeneity at every turn.Provide by Animal Experimental Study chamber, Traditional Chinese Medicine Research Institute, Sichuan Province, conform with primary standard (economizing real moving pipe No. 32).

The big ear outbreeding system white rabbit of Japan, about 4 monthly ages, body weight 2.5 ± 0.5kg, male and female all have, physical health, test dislocation laboratory the last week freely absorbs the standard particle feedstuff, and grass meal and tap water are provided by Animal Experimental Study chamber, Traditional Chinese Medicine Research Institute, Sichuan Province.

1.5 test key instrument

Mouse cage at the bottom of CS501 type ultrathermostat and the red copper, Chongqing testing equipment instrument plant of the People's Republic of China (PRC) makes.

2, experimental technique and result

2.1 " this electuary " is to thermostimulation method (hot plate method ⁽¹⁾) cause the influence of mice pain reaction.

Get some of female mices, select its pain threshold values person in 25 seconds and use for experiment.Get and select 50 of qualified mices, be divided into 5 groups at random, every group is 10, be respectively the normal saline matched group, rotundine 20mg/kg, " this electuary " 50,25,12.5g/kg, all irritate stomach (1g) administration, every group is worth twice in the normal pain of its each Mus of administration before measurement, with average as normal threshold values bitterly.Respectively measured the once pain threshold values of every Mus in 30,60,90,120,180 minutes after the administration, experimental data is learned processing by statistics, and its result sees table 1 for details.

Annotate: the pain threshold values: promptly mice is placed on that it licks the abnormal movement of back vola as a kind of acute discomfort on 55 ± 0.1 ℃ the hot plate, and thermostimulation begins to be the pain threshold values to the time of licking between the foot.

Table 1 " this electuary " causes the influence of mice pain sensation reaction to thermostimulation

Pain threshold values behind the normal pain of the dosage Mus number valve medicine (second X ± SD)

Group

G/kg (only) value 30 60 90 120 180 minutes

(second X ± SD)

Normal saline 10 21.1 22.9 22.4 22.7 20.4 20.4

±5.4 ±4.9 ±5.1 ±4.4?±4.1 ±4.2

※※ ※※ ※※ ※※

Rotundine Tablets 0.02 10 21.1 25.5 35.2 38.2 53.4 47.0

±5.0 ±8.4 ±10.1?±8.6?±10.2?±10.7

※※ ※※ ※※ ※※

This electuary 50 10 20.3 43.8 46.1 38.6 30.0 23.8

±5.9 ±14.2 ±13.2?±12.3±5.4 ±5.3

※※ ※※ ※※

25 10 19.4 30.8 38.5 34.3 31.7 23.1

±3.8 ±7.4 ±6.9 ±7.4?±5.6 ±5.1

※ ※※

12.5 10 20.5 28.2 32.0 30.4 26.9 21.7

±3.7 ±8.3 ±10.6?±5.6?±6.9 ±4.8

Annotate: with the normal saline contrast ratio: ※ P＜0.05, ※ ※ P＜0.01.

Table 1 is the result show, each group of " this electuary " 50,25,12.5g/kg causes mice pain to thermostimulation significant analgesic activity, reaches the effect peak in 30-90 minute behind the medicine, continues more than 2 hours, and it is more obvious to increase analgesic effect with dosage.This electuary and rotundine 20mg/kg relatively, its analgesic activity onset is very fast, but action intensity and persistent period are like a little less than.

2.2 " this electuary " Dichlorodiphenyl Acetate causes the influence of mouse writhing reaction

Get 50 of male mices, random packet is respectively the normal saline matched group, rotundine 20mg/kg group and this electuary 50,25,12.5g/kg group, totally five groups, 10 of every group of mices all do the 1g administration, after 1 hour, each organizes every animal lumbar injection (1g) 0.7% acetate solution 0.2ml/20g Mus, immediately in the observed and recorded 20 minutes, and the writhing response number of times of each Mus, the gained data are learned processing by statistics, and its result sees table 2 for details.

Table 2 " this electuary " Dichlorodiphenyl Acetate causes the influence of little oxygen writhing response

Dosage number of animals writhing response number of times

Group

Suppression ratio (%) P value

G/kg (only) (X ± SD)

Normal saline 10 45.1 ± 6.6

Rotundine 0.02 10 20.5 ± 11.2 54＜0.01

This electuary 50 10 19.3 ± 6.7 57＜0.01

25 10 21.1±15.5 53 ＜0.01

12.5 10 23.0±17.4 49 ＜0.05

Table 2 is the result show, this electuary 50,25,12.5/kg Dichlorodiphenyl Acetate cause the mouse writhing reaction significant analgesic activity, and the doses dependence is arranged, and the analgesic activity of this electuary 50,25g/kg and rotundine 20mg/kg is close.

2.3 the analgesic activity of each extraction process of we relatively

Experimental result according to 2.1,2.2 shows: " this electuary " causes mice pain that certain analgesic activity is all arranged to thermostimulation and chemical stimulation.Therefore relatively adopt during the production technology of " electuary " acetic acid to cause the electuary that different process produces is observed in the mouse writhing reaction as pain model analgesic activity, the extract powder of five kinds of technology extractions is all to contain crude drug in whole conversion dosage, the equal 1g 50g/kg of every medicine, experimental technique is with 2.2, and it the results are shown in Table 3.

This electuary Dichlorodiphenyl Acetate that table 3 different process is made causes the influence of mouse writhing reaction

Writhing response

The group number of animals is counted the explanation of suppression ratio P value

(only) (X ± SD) (%)

Normal saline 17 45.2 ± 22.1

This electuary technology 10 28.8 ± 21.4 36＞0.05 decocting in water

I

This electuary technology 10 36.3 ± 16.3 20＞0.05 alcohol leaching

II

This electuary technology 10 23.5 ± 10.9 48＜0.05 water alcohol add ring

III

This electuary technology 10 24.1 ± 17.1 47＜0.06 water alcohol add to be waved

IV

This electuary technology 10 22.2 ± 16.4 51＜0.05 macroporous resins

V

Table 3 is the result show: this electuary preparation process III, IV, V all have significant analgesia role, and analgesic effect difference each other is little, prepare this electuary according to the IV of the easy consideration system of selection of processing technology as manufacturing technique method.

2.4 one of antiinflammatory action of this electuary, on Carrageenan cause the influence (antiinflammatory action) of mice swelling

Get 60 of male mices, body weight 20 ± 1g is divided into five groups at random, except that the contrast 20 10 every group, be respectively the normal saline matched group, indometacin 20mg/kg, this electuary 50,25,12.5g/kg group, equal 1g, administration is after 1 hour, the strange hymeniderm in back is injected (SC) 1% carrageenin 0.05ml down about every mice, taking off vertebra again after 2 hours and put to death mice, cut biped in the place by 2mm on the ankle joint, is paw swelling with biped weight difference (right side-left side), the experimental data statistical procedures, its result sees table 4 for details.

The influence of the foot swelling that this electuary of table 4 on Carrageenan causes

Number of animals swelling degree suppression ratio

Group dosage g/kg P value

(only) (mg X ± SD) (%)

Normal saline 20 186.3 ± 12.2

Indometacin 0.02 10 92.5 ± 20.0 50＜0.01

This electuary 50 10 52.0 ± 9.2 72＜0.01

25 10 78.0±19.5 61 ＜0.01

12.5 10 135.5±14.4 27 ＜0.01

Table 4 is the result show, this electuary 50,25,12.5g/kg all have certain inhibition angle to pitch the mice foot swelling effect that certain glue causes, and as seen the doses dependence is arranged.Its detumescence effect, this electuary 50g/kg is imitated scorching pain 20mg/kg and also is eager to excel.

2.5 this electuary is to the refrigeration function that causes fever in rabbit (heat clearing away effect) of triple vaccine

Get some of the big ear rabbit of Japan, male and female all have, body weight 2.1 ± 0.6kg measures normal anus temperature for several times before the test, select that it is constant between 38.5～39.5 ℃, get 20 confessions and try out.Every then rabbit is by body weight intravenous injection (1g) typhoid fever, paratyphoid fever, second triple vaccine liquid 1.0ml/kg, and measure the anus temperature per then half an hour one time, and mean body temperature raises more than 1 ℃ after one hour, be heating body temperature this moment, be divided into 4 groups at random, 5 every group, be respectively a reason saline control group (1g); Injection of Radix Bupleuri 0.5g/kg organizes (1m); This electuary 30,10g/kg organize (1g), respectively survey body temperature one time in 30,60,120,180,240 minutes behind medicine, and experimental data is learned processing by statistics, sees table 5 for details.

This electuary of table 5 is to the influence of trimerization vaccine fever in rabbit body temperature (X ± SD)

After the injection of dosage number of animals body weight basis body injection bacterium (branch)

Group

G/kg (only) kg temperature (℃) Seedling (℃) 30 60 120 180 240

Normal saline 5 2.3 38.9 40.0 40.2 40.3 40.2 39.3 39.0

±0.6 ±0.2 ±0.2 ±0.5 ±0.3 ±0.4 ±0.3 ±0.2

Radix Bupleuri 0.5 5 2.3 38.9 40.2 39.6 39.5 39.9 39.8 38.9

±0.2 ±0.5 ±0.6 ±6 ±0.2 ±0.5 ±0.2 ±0.4

This electuary 30 5 2.2 39.1 40.0 39.7 39.5* 39.8 39.9 39.6

±0.3 ±0.4 ±0.4 ±0.5 ±0.3 ±0.6 ±0.5 ±0.6

10 5 2.2 38.8 39.9 39.7 39.6 39.9 39.9 39.3

±0.3 ±0.5 ±0.6 ±0.6 ±0.7 ±0.4 ±0.3 ±0.5

Annotate: with normal saline contrast ratio * P＜0.05 same period

Table 5 is the result show, the fever in rabbit body temperature rise that this electuary 30g/kg caused triple vaccine after administration in 60 minutes has certain refrigeration function, and its effect is suitable with injection of Radix Bupleuri.

3, evaluation of result and discussion

Show that by Preliminary pharmacological test " Yanning adjuvant electuary " all has analgesic activity significantly to the mice pain reaction that thermostimulation and chemical stimulation cause; The mice foot swelling that on Carrageenan causes has detumescence (antiinflammatory) effect very significantly; But the fever in rabbits that typhoid fever, paratyphoid fever, second triple vaccine are caused has faint refrigeration function.

Claims (2)

1. * claimed following prescription:

   1, the Yanning adjuvant electuary is formed:

   Radix Sophorae Tonkinensis 8g Radix Platycodonis 10g Bombyx Batryticatus 8g

   Cortex Moutan 9g Rhizoma Belamcandae 7g Herba Schizonepetae 9g

   Radix Saposhnikoviae 8g Flos Chrysanthemi 8g Herba Menthae 4g

   Radix Scrophulariae 12g Radix Rehmanniae 12g Fructus Forsythiae 10g

   Radix Glycyrrhizae 7g Bulbus Fritillariae Thunbergii 8g
2. 2, Yanning adjuvant powder is formed:

   Borneolum Syntheticum 3.5g Borax 4.0g Concha Margaritifera (forging) 7g

   Mentholum 4.0g Indigo Naturalis 1.5g Gypsum Fibrosum Preparatum 7g

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