CN1500878A - Highly effective method for getting the active ingredient in ganoderma lucidum spore - Google Patents
Highly effective method for getting the active ingredient in ganoderma lucidum spore Download PDFInfo
- Publication number
- CN1500878A CN1500878A CNA02148743XA CN02148743A CN1500878A CN 1500878 A CN1500878 A CN 1500878A CN A02148743X A CNA02148743X A CN A02148743XA CN 02148743 A CN02148743 A CN 02148743A CN 1500878 A CN1500878 A CN 1500878A
- Authority
- CN
- China
- Prior art keywords
- ganoderma spore
- glossy ganoderma
- enzyme
- present
- active ingredient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides one efficient and fast biological enzymolysis process of breaking glossy ganoderma spore cell wall to obtain its bioactive matter. Glossy ganoderma spore contains rich bioactive matter and has very high nourishing and health care effect, but its hard cell wall structure blocks its function, so that people have always seeking the solution of well breaking glossy ganoderma spore cell. The glossy ganoderma spore cell consists of chitosan, beta-1, 3-glucosan, protein and other matters, and the present invention utilizes special enzyme to enzymolyze glossy ganoderma spore cell based on its chemical composition and structural features. The present invention has the features of high wall breaking efficiency, mild enzyme reaction, excellent protection of bioactive components, etc.
Description
Technical field: the present invention relates to biochemical field, be specifically related to the Ganoderma spore cell walls is carried out enzymolysis, efficiently to obtain the processing method of biologically active substance.
Background technology: Ganoderma spore powder is rich in high-load active ingredient, has many-sided nourishing function, but its hard cell walls can not be made effective ingredient be difficult to be absorbed by body by pipe intestinal digesting.Be the function of performance Ganoderma spore, wall-breaking method has at present: 1, physics method.Comprise method broken walls such as use is milled, ultrasonic wave, microwave, supersonic airstream, impressed pressure, these technologies are comparatively complicated, need by mechanical means, and shell-broken effect is limited; 2, chemical method.Because soda acid and other chemical reagent can destroy biological activities such as polysaccharide, protein, so the use of this method is restricted; 3, biochemical process.Method with enzymolysis is abolished cell walls, but known at present reported method do not form and do not select zymin for use at the structure of Ganoderma spore, thereby efficient is low, also must uniting in conjunction with additive methods such as ultrasonic wave of having carried out, technology is loaded down with trivial details.
The Ganoderma spore cell walls mainly is made up of chitin, dextran, neutral polysaccharide, protein, lipoid etc.Chitin forms cell wall skeleton with web form, and filling dextran to keep hard cell wall structure, the organism and the inorganicss such as calcium and phosphoric acid such as polyose, protein and lipoid in addition of filling mesh in cancellated netted hole.Chitin is to get up not have the straight chain polymer of side chain by N-acetyl-D glycosamine with β-1.4-key joint, is the effect substrate of chitinase.The dextran of conidial cell wall be glucosyl residue by the polysaccharide that β-the 1.3-key couples together, be the substrate of interior-β-1.3-dextranase effect.Those of ordinary skill in the art knows, enzyme is the class protein that is produced by cell (comprising microorganism), can be in cell or the extracellular play katalysis, so claim biological catalyst again, the enzymic catalytic reaction gentleness has significant two characteristics, i.e. high-level efficiency and specificity.The specificity of enzyme shows three aspects, and some enzyme can only a kind of substrate of catalysis, carries out a kind of reaction; Some enzyme all has katalysis to same compounds or chemical bond; Also some enzyme can only a kind of steric isomer of catalysis, and another kind of isomer is not had effect.Interior-β-1.3-dextranase only works to β-1.3-glucose key, and chitinase only works to chitin.Proteolytic enzyme can only decomposing protein.The present invention comes the Ganoderma spore cell walls is carried out the method for efficiently obtaining its active ingredient that enzymolysis proposes according to these characteristics of enzyme just.
Summary of the invention: the present invention selects based on interior-β-1.3-dextranase according to the chemical constitution and the basic structure of Ganoderma spore, is auxilliary with chitinase, proteolytic enzyme, forms a plurality of reaction systems.Ganoderma spore and above-mentioned arbitrary enzyme reaction system are mixed, act on certain hour at a certain temperature, the centre is repeatedly fully stirred, and can reach the purpose that directly cracks cell walls fully.The protoplastis that forms behind the broken wall is very easily destroyed by gi tract, and causing its activeconstituents is that body absorbs, utilizes.The present invention compares with the physics method, have easy and simple to handle, need not mechanical means, simple, the consuming time weak point of technology, broken wall efficient advantages of higher; Compare with chemical method, have the advantage that its active ingredient is greatly protected, and deleterious other the residual compositions of unmatchful body; Compare with other biochemical processs, pointed strong, sporoderm-broken rate is high, the advantage that need not secondary processing.
The present invention also provides a kind of zymin of enzymolysis Ganoderma spore, utilizes the own broken wall of this preparation human consumer, and oneself uses product, and the human consumer is more relieved to guaranteeing the quality of product.
The general temperature condition of method of enzymolysis Ganoderma spore wall provided by the invention is 20-60 ℃, preferred 35-45 ℃.The enzyme reaction system is: in (1)-and β-1.3-dextranase, in (2)-β-1.3-dextranase+proteolytic enzyme, in (3)-β-1.3-dextranase+chitinase, in (4)-β-1.3-dextranase+chitinase+proteolytic enzyme.Identical its shell-broken effect of other conditions is (4)＞(3)＞(2)＞(1).Enzyme concn is 0.5%---8%, and enzymolysis time is 1-10 hour, generally is no less than 1 hour.Enzymolysis time is relevant with temperature and enzyme concn, and too high or too low for temperature, enzymic activity reduces, and enzymolysis time is long; Enzyme concn height, enzymolysis time are just short.In selecting generally speaking-and β-1.3-dextranase, sporoderm-broken rate reaches more than 65%; In the choosing-and β-1.3-dextranase+proteolytic enzyme, sporoderm-broken rate reaches more than 80%; In the choosing-and β-1.3-dextranase+chitinase, sporoderm-broken rate reaches more than 90%; In the choosing-and β-1.3-dextranase+chitinase+proteolytic enzyme, sporoderm-broken rate reaches more than 95%.
Specific embodiments:
Embodiment 1
Got 2 kairine Ganoderma lucidum spore powder warm water soaking 24 hours, centrifugal collection back moves into 100ml, in the 0.4%mol/L sucrase liquid (in 2%-β-1.3-dextranase+2% chitinase); 40 ℃ were reacted 5 hours, per hour fully shake once, and each 5 minutes, under ordinary optical microscope, can check out shell-broken effect, sporoderm-broken rate can reach more than 90%.This product can directly be used, but also efficient Ganoderma spore powder is made in vacuum-drying.
Claims (5)
1. a method of obtaining the Ganoderma spore active ingredient is characterized in that using the enzyme of selecting for use at Ganoderma spore cell wall structure composition to carry out broken wall.
2. according to the method for claim 1, the enzyme that it is characterized in that described broken wall is interior-β-1.3-dextranase.
3. according to the method for claim 2, the enzyme that it is characterized in that described broken wall is a proteolytic enzyme.
4. according to the method for claim 2, the enzyme that it is characterized in that described broken wall is a chitinase.
5. according to the method for claim 4, the enzyme that it is characterized in that described broken wall is a proteolytic enzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA02148743XA CN1500878A (en) | 2002-11-15 | 2002-11-15 | Highly effective method for getting the active ingredient in ganoderma lucidum spore |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA02148743XA CN1500878A (en) | 2002-11-15 | 2002-11-15 | Highly effective method for getting the active ingredient in ganoderma lucidum spore |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1500878A true CN1500878A (en) | 2004-06-02 |
Family
ID=34233306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA02148743XA Pending CN1500878A (en) | 2002-11-15 | 2002-11-15 | Highly effective method for getting the active ingredient in ganoderma lucidum spore |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1500878A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755828A (en) * | 2014-01-16 | 2014-04-30 | 中国科学院上海有机化学研究所 | Ganoderma lucidum spore polysaccharide and preparation method and application thereof |
-
2002
- 2002-11-15 CN CNA02148743XA patent/CN1500878A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755828A (en) * | 2014-01-16 | 2014-04-30 | 中国科学院上海有机化学研究所 | Ganoderma lucidum spore polysaccharide and preparation method and application thereof |
| CN103755828B (en) * | 2014-01-16 | 2015-11-18 | 中国科学院上海有机化学研究所 | Ganoderma spore polysaccharide, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ezeilo et al. | Enzymatic breakdown of lignocellulosic biomass: the role of glycosyl hydrolases and lytic polysaccharide monooxygenases | |
| Chen et al. | N-acetylglucosamine: production and applications | |
| WO2001049830A3 (en) | Improved lysosomal enzymes and lysosomal enzyme activators | |
| CN114901701A (en) | Ultra-low molecular weight hyaluronic acid and preparation method thereof | |
| CN101304724A (en) | Hyaluronidase inhibitor containing poly-gamma-glutamic acid as an effective component | |
| EP1483299A1 (en) | Cell wall derivatives from biomass and preparation thereof | |
| CN106832332B (en) | Overactivity degree humic acid and its production method | |
| CN107652373B (en) | A kind of preparation method of low-molecular weight chitoglycan | |
| CN106146156A (en) | A kind of organic-inorganic polymerization water keeping fertilizer and preparation method thereof | |
| CN102199592A (en) | Method for preparing mixed immobilized glucose oxidase/catalase microspheres | |
| Wang et al. | Cellulase immobilized by sodium alginate-polyethylene glycol-chitosan for hydrolysis enhancement of microcrystalline cellulose | |
| CN109336991A (en) | A kind of extracting method of hericium erinaceum polysaccharide | |
| CN110201530A (en) | A kind of purification air active ingredient and preparation method thereof | |
| CN101921671A (en) | Bio-enzyme cleaning agent for medical apparatuses and preparation method thereof | |
| CN1230219A (en) | Detection of mammalian heparanase activity and purification of mammalian heparanase | |
| CN1500878A (en) | Highly effective method for getting the active ingredient in ganoderma lucidum spore | |
| CN102453707A (en) | Preparation method of immobilized cellulase | |
| Hu et al. | Xylanase-polymer conjugates as new catalysts for xylooligosaccharides production from lignocellulose | |
| CN106957835A (en) | A kind of reaction solution reacted for trehalose synthase | |
| JPS63213501A (en) | Production of pectin | |
| Struszczyk | Chitin and chitosan. Part III. Some aspects of biodegradation and bioactivity | |
| CN102422848B (en) | Preparation method of immobilized glucose oxidase with muscle strengthening function in production process of bread | |
| CN109136214A (en) | A kind of preparation method and application of fixing lactic acid bacteria | |
| CN114560957A (en) | Method for extracting polygonatum odoratum polysaccharide | |
| CN104828895B (en) | A kind of method for treating water of enzymolysis xylogen using oxidation modified zirconia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |