CN106957835A - A kind of reaction solution reacted for trehalose synthase - Google Patents
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- C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
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Abstract
The present invention relates to a kind of application of reaction solution reacted for trehalose synthase and the reaction solution in trehalose synthase converts maltose generation trehalose.Reaction solution of the present invention contains glucose oxidase, when for trehalose synthase conversion maltose generation trehalose, the accessory substance glucose of trehalose synthase reaction can be removed, it is to avoid suppression of the glucose to trehalose synthase activity, improve reaction efficiency and trehalose conversion ratio.
Description
Technical field
The invention belongs to biological technical field, it is related to a kind of reaction solution and the reaction solution reacted for trehalose synthase
Application in trehalose synthase converts maltose generation trehalose.
Background technology
Trehalose is the irreducibility disaccharide being formed by connecting by two glucose molecules with α -1,1 glycosidic bonds, is widely present
In rudimentary plant, algae, bacterium, fungi, yeast and insect, many species adverse circumstances to external world（Dehydration, arid, high temperature,
Freezing, hyperosmosis and toxic reagent etc.）The degeneration-resistant tolerance shown has direct relation with their internal trehaloses, and
And exogenous trehalose equally has good non-specific protective effect to biomembrane and large biological molecule, this uniqueness
Biological function makes trehalose gather around in fields such as food, cosmetics, molecular biology, medical science, agriculturals to have broad application prospects.
For example, trehalose can be used as biological products and the protective agent and stabilizer of active bacteria formulation；Also food can be effectively improved as one kind
The natural additive for foodstuff of quality and local flavor；It can be additionally used in cosmetics, protect the skin from Exposure to Sunlight and freezing injury, prevent
Dry skin etc..Food and drug administration authorizes trehalose GRAS in October, 2000（It is known as safety）Status,
And ratify each field that it enters U.S.'s food.Resolution is made by September, 2001, EU Committee, and approval trehalose is in state of European Union
Used in family as novel foodstuff.In March, 2005, ministry of Health of China approval trehalose is new resource food.
The method of current large-scale production trehalose is to utilize malt oligosaccharide based mycose synthetase and Fructus Hordei Germinatus oligose Ji Hai
Algae glycosylhydrolase concerted catalysis Fructus Hordei Germinatus oligose generates trehalose, and the trehalose price produced is higher, limits the wide of trehalose
General application, in the urgent need to researching and developing new technology.
Trehalose synthase can be catalyzed maltose generation intramolecular and turn glucosides reaction generation trehalose, reaction substrate maltose
Obtained by amylorrhexis, source is sufficient, is hopeful to produce trehalose using the enzyme low cost.Therefore both at home and abroad in bacterium producing multi enzyme preparation sieve
Many work are carried out in terms of choosing, engineering bacteria structure, enzyme reaction.
But trehalose synthase produces glucose accessory substance, reaction temperature during conversion maltose generation trehalose
Higher, the formation speed of glucose is faster, and growing amount is higher.Trehalose conversion ratio so can be not only reduced, and glucose is
The inhibitor of trehalose synthase, can significantly reduce trehalose synthase conversion reaction speed.Further, since the presence of glucose, also
Difficulty and cost that product is isolated and purified can be increased.Above mentioned problem constrains the scale application of trehalose synthase.
The content of the invention
Not enough present in above-mentioned technology to solve, first purpose of the invention is to provide a kind of for trehalose synthase
The reaction solution of reaction, in the reaction solution, glucose can be removed；Second object of the present invention is that the reaction solution is used for into sea
Algae sugar synthase conversion maltose generation trehalose, improves the reaction efficiency of trehalose synthase.
In order to achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of reaction solution reacted for trehalose synthase, the reaction solution is except containing trehalose synthase, maltose and buffering
Beyond liquid, also contain glucose oxidase.
The reaction solution contains catalase.
The pH value of the reaction solution is 5 ~ 8.
Application of the described reaction solution in trehalose synthase converts maltose generation trehalose.
The present invention has the following advantages that compared with oneself has technology:
Reaction solution of the present invention contains glucose oxidase, when for trehalose synthase conversion maltose generation trehalose, reaction
Trehalose synthase can be reacted the accessory substance glucose produced and be oxidized to gluconic acid by the glucose oxidase in liquid, so as to go
Except glucose, it is to avoid suppression of the glucose to trehalose synthase activity, accelerate reaction process, improve reaction efficiency and trehalose turns
Rate.In addition, after reaction terminates, only two kinds of sugar containing trehalose and maltose, trehalose proportion in two kinds of sugar in product
Greatly, it is easy to further isolate and purify trehalose.Above-mentioned advantage can be reduced produces being produced into for trehalose using trehalose synthase
This, is laid the foundation to realize using trehalose synthase prepare with scale trehalose.
Embodiment
The present invention is further described in conjunction with the embodiments.It should be understood that these embodiments are merely to illustrate the present invention rather than limit
The scope of the present invention processed.
A kind of reaction solution reacted for trehalose synthase, the reaction solution containing trehalose synthase, maltose except easing up
Beyond fliud flushing, also contain glucose oxidase.Trehalose synthase and glucose oxidase can be resolvase or fixation
Change enzyme.The immobilised enzymes refers to that enzyme molecule is combined with other materials or enzyme molecule is bonded to each other or enzyme molecule is included in other
Water insoluble enzyme formed by material.Content and maltose concentration to trehalose synthase in reaction solution are not construed as limiting, can
Determine as needed.The content of glucose oxidase should be enough in reaction solution, so as to fully degraded trehalose synthase reaction
When the accessory substance glucose that produces, can pass through to test and determine suitable glucose oxidase content.Species to buffer solution and dense
Degree is not limited, as long as not influenceing or having substantially no effect on trehalose synthase and the activity of glucose oxidase, generally can be
0.01 ~ 0.1M phosphate buffer.
The enzyme activity of the content available units volume of trehalose synthase and glucose oxidase represents, enzyme activity determination method and enzyme
Unit living（U）It is defined as follows：
The enzyme activity determination method of trehalose synthase is：By lmL liquid enzymes（Or lmL immobilised enzymes suspension）Be added to lmL with
The 20% of 10mM, pH6.5 phosphate buffer preparation（w/v）Maltose solution in, after mixing, by the mixture solution in 50
DEG C insulation 60min, then in 100 DEG C heat 10min stop the enzymatic reaction.After reactant mixture cooling, centrifugation（8000r/
Min, 15min）, supernatant is taken, the content of trehalose generated with high effective liquid chromatography for measuring.Enzyme-activity unit is defined as：Upper
State under reaction condition, the enzyme amount needed for conversion maltose per minute generates 1 μm of ol trehalose is 1 enzyme-activity unit（U）.
The enzyme activity of glucose oxidase is determined as follows：0.1mL enzyme liquids are taken, 0.1mL, 0.5% is added（w/v）Portugal
Grape sugar（With 50mM, pH6.5 phosphate buffered saline）, 10min is reacted at 50 DEG C, Grape by HPLC is used
Sugared content.Enzyme-activity unit is defined as：Under the above-described reaction conditions, the enzyme amount needed for 1 μm of ol glucose of decomposition per minute is 1 enzyme
Unit living（U）.
The condition of Determination of Trehalose by HPLC, maltose and glucose is as follows：
Chromatographic column：Hypersil NH2, 4.6mm × 250mm, 5 μm of packing material size；Detector：Differential refraction detector；Flowing
Phase：Acetonitrile/water=75/25（Volume ratio）；Column temperature：Room temperature；Sample size：20μL；Flow velocity：1.0mL/min.
Also contain catalase in the reaction solution of the present invention.The content of catalase should be enough, so as to fully
The hydrogen peroxide that degraded glucose oxidase catalysis grape is generated when glycoxidative, it is to avoid hydrogen peroxide influences trehalose synthase and Portugal
The activity of grape carbohydrate oxidase.Suitable catalase content can be determined by testing.The content available units of catalase
The enzyme activity of volume represents, enzyme activity determination method and enzyme-activity unit（U）It is defined as follows：
2.9mL, 0.1mol/L hydrogenperoxide steam generator are added in quartz cuvette（Matched somebody with somebody with 20mM, pH6.5 phosphate buffer
System）With 0.1mL enzyme liquids, with the decomposition rate of colorimetric method for determining hydrogen peroxide at 240nm.Enzyme-activity unit is defined as：Above-mentioned
Under reaction condition, the enzyme amount needed for 1 μm of ol of decomposition per minute hydrogen peroxide is 1 enzyme-activity unit（U）.
The content of hydrogen peroxide can be used " in GB 5009.226-2016 national food safety standard food in reaction solution
The measure of residual quantities of hydrogen peroxide " method is detected.
The pH value of reaction solution of the present invention is 5 ~ 8.Buffer solution that the pH value is contained by reaction solution is realized.In the pH value range
Interior, trehalose synthase, glucose oxidase and catalase can keep wholly or largely active.Preferable ph is trehalose
The optimal reaction pH value of synthase, usually 6 ~ 7.
, first will be anti-when the reaction solution using the present invention carries out the reaction of trehalose synthase conversion maltose generation trehalose
Liquid is answered to be placed in reactor.It can select stirring-type, bubble type or fluidized-bed reactor.Available reaction temperature is that will not make marine alga
The temperature of sugared synthase, glucose oxidase and catalase fast deactivation, preferred range is less than 70 DEG C.Course of reaction
In, the pH value of reaction solution remains constant with pH-stat methods.The reaction time used is according to reaction condition and required trehalose conversion ratio
And select, generally optional trehalose conversion ratio reaches reaction time during high value.Trehalose conversion ratio is defined as reaction life
Into trehalose and reaction starting maltose used percentage.After reaction terminates, it can adopt and divide by any known method
From, Purifing Trehalose.
Embodiment one
A kind of reaction solution reacted for trehalose synthase：
The content of trehalose synthase is 300U/L in reaction solution（I.e. every liter 300 enzyme-activity units）, maltose concentration is 50g/L,
The content of glucose oxidase is 200U/L（I.e. every liter 200 enzyme-activity units）, the content of catalase is 230U/L（It is i.e. every
Rise 230 enzyme-activity units）, the concentration of phosphate buffer is 50mM, and the pH value of reaction solution is 6.5.
Embodiment two
A kind of reaction solution reacted for trehalose synthase：
The content of glucose oxidase is 600U/L in reaction solution（I.e. every liter 600 enzyme-activity units）, the content of catalase
For 690U/L（I.e. every liter 690 enzyme-activity units）, other be the same as Examples one.
Embodiment three
Application of the reaction solution of the present invention in trehalose synthase converts maltose generation trehalose：
With the reaction solution of embodiment one, 72h is reacted at 30 DEG C in stirring reactor, speed of agitator is 50r/min, during which
Respectively at glucose, trehalose and content of hydrogen peroxide in 4h, 12h, 24h, 48h and 72h sampling, detection reaction solution, sea is calculated
Algae sugar conversion ratio.As a result show, during reaction 4h, 12h, 24h, 48h and 72h, glucose and peroxide are not detected in reaction solution
Change hydrogen.In addition, during reaction 24h, trehalose conversion ratio is 64.3%；When reacting 48h, trehalose conversion ratio reaches highest, is
66.2%；When reacting 72h, trehalose conversion ratio is 60.8%.Therefore, during 30 DEG C of reactions, reaction 48h is advisable.
Example IV
Application of the reaction solution of the present invention in trehalose synthase converts maltose generation trehalose：
With the reaction solution of embodiment two, 72h is reacted at 50 DEG C in stirring reactor, speed of agitator is 50r/min, during which
Respectively at glucose, maltose, trehalose and content of hydrogen peroxide in 4h, 12h, 24h, 48h and 72h sampling, detection reaction solution,
Calculate trehalose conversion ratio.As a result show, during reaction 4h, 12h, 24h, 48h and 72h, glucose is not detected in reaction solution
And hydrogen peroxide.When reacting 12h, trehalose conversion ratio is 60.9%；When reacting 24h, trehalose conversion ratio reaches highest, is
62.9%；When reacting 48h and 72h, trehalose conversion ratio declines.Therefore, during 50 DEG C of reactions, reaction 24h is advisable.In addition, reaction
During 24h, only two kinds of sugar containing trehalose and maltose in reaction solution, trehalose accounts for 75% in two kinds of sugar, is easy to further to marine alga
Sugar is isolated and purified.
Comparative example one
In this comparative example, the reaction solution of use is other with real in addition to not containing glucose oxidase and catalase
Apply example three.Reacted with the reaction solution, reaction condition be the same as Example three.As a result show, with the extension in reaction time, reaction
Glucose content gradually increases in liquid.When reacting 24h, glucose content about 1.6g/L in reaction solution；When reacting 48h, glucose
Content about 2.7g/L；When reacting 72h, glucose content reaches 3.5g/L.Illustrate trehalose synthase in conversion maltose generation sea
Glucose accessory substance is generated during algae sugar.In addition, during reaction 24h, trehalose conversion ratio is 53.5%；When reacting 72h,
Trehalose conversion ratio highest, about 63%.Compared with embodiment three and understood, because trehalose synthase reacts by-product in this comparative example
The presence of thing glucose, has slowed down trehalose synthase conversion reaction speed, have impact on reaction efficiency, also reduces trehalose conversion
Rate.
Comparative example two
In this comparative example, the reaction solution of use is other with real in addition to not containing glucose oxidase and catalase
Apply example four.Reacted with the reaction solution, reaction condition be the same as Example four.As a result show, with the extension in reaction time, reaction solution
Middle glucose content is dramatically increased.When reacting 12h, glucose content about 2.2g/L in reaction solution；When reacting 24h, glucose contains
Measure about 3.9g/L；When reacting 72h, glucose content reaches 10g/L.Illustrate trehalose synthase in conversion maltose generation trehalose
During not only produce glucose accessory substance, and because reaction temperature is improved（Compared with comparative example one）, the life of glucose
Accelerate into speed.
In addition, in this comparative example, during reaction 24h, glucose, three kinds of sugar of trehalose and maltose, sea are contained in reaction solution
Algae sugar accounts for 53% in three kinds of sugar.Compared with example IV and understood, this comparative example sugar type is more, and trehalose institute in total reducing sugar
Accounting example is low, and this will increase the difficulty and cost that isolate and purify trehalose.
Further, in this comparative example, during reaction 12h, trehalose conversion ratio is 46.9%；When reacting 24h, trehalose conversion ratio
Highest is reached, is 53%；When reacting 48h and 72h, trehalose conversion ratio declines.And in example IV, during reaction 12h, sea
Algae sugar conversion ratio has just reached 60.9%.Illustrate in reaction solution of the present invention due to the glucose oxidase containing sufficient amount, Neng Gouchong
Divide and remove the accessory substance glucose that trehalose synthase is produced during conversion maltose generation trehalose, so as to avoid grape
Suppression of the sugar to trehalose synthase activity, accelerates reaction speed, improves reaction efficiency and trehalose conversion ratio.
Claims (4)
1. a kind of reaction solution reacted for trehalose synthase, it is characterised in that：The reaction solution except containing trehalose synthase,
Beyond maltose and buffer solution, also contain glucose oxidase.
2. it is used for the reaction solution that trehalose synthase reacts described in claim 1, it is characterised in that：The reaction solution contains peroxide
Change hydrogen enzyme.
3. it is used for the reaction solution that trehalose synthase reacts described in claim 1, it is characterised in that：The pH value of the reaction solution is
5~8。
4. the reaction solution in claims 1 to 3 described in any claim is in trehalose synthase conversion maltose generation trehalose
In application.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642038A (en) * | 2018-06-20 | 2018-10-12 | 天津市林业果树研究所 | A kind of gel immobilized enzyme and preparation method thereof having both catalysis and antibacterial functions |
| CN111850069A (en) * | 2020-07-27 | 2020-10-30 | 溧阳维信生物科技有限公司 | Production and preparation process of trehalose |
| CN113913483A (en) * | 2021-11-23 | 2022-01-11 | 常州大学 | Method for co-producing trehalose and gluconic acid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106244647A (en) * | 2016-08-24 | 2016-12-21 | 山东福洋生物科技有限公司 | A kind of method simultaneously preparing trehalose and gluconic acid lactone |
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2017
- 2017-04-28 CN CN201710293967.7A patent/CN106957835A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244647A (en) * | 2016-08-24 | 2016-12-21 | 山东福洋生物科技有限公司 | A kind of method simultaneously preparing trehalose and gluconic acid lactone |
Non-Patent Citations (3)
| Title |
|---|
| TSUNG-TA WU ET AL.: "Enzymatic Processes for the Purification of Trehalose", 《AMERICAN INSTITUTE OF CHEMICAL ENGINEERS》 * |
| TSUNG-TA WU ET AL.: "Selective oxidation of glucose for facilitated trehalose purification", 《PROCESS BIOCHEMISTRY》 * |
| 吴秀丽等: "海藻糖合酶的研究进展", 《微生物学通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642038A (en) * | 2018-06-20 | 2018-10-12 | 天津市林业果树研究所 | A kind of gel immobilized enzyme and preparation method thereof having both catalysis and antibacterial functions |
| CN111850069A (en) * | 2020-07-27 | 2020-10-30 | 溧阳维信生物科技有限公司 | Production and preparation process of trehalose |
| CN113913483A (en) * | 2021-11-23 | 2022-01-11 | 常州大学 | Method for co-producing trehalose and gluconic acid |
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