CN1500812A - Purifying method of high molecular weight water soluble chitin derivant - Google Patents
Purifying method of high molecular weight water soluble chitin derivant Download PDFInfo
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- CN1500812A CN1500812A CNA021519668A CN02151966A CN1500812A CN 1500812 A CN1500812 A CN 1500812A CN A021519668 A CNA021519668 A CN A021519668A CN 02151966 A CN02151966 A CN 02151966A CN 1500812 A CN1500812 A CN 1500812A
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- molecular weight
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- weight water
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Abstract
The purification process for high molecular weight water soluble chitin derivative is suitable for chemical modification of chitin to prepare high molecular weight water soluble chitin derivative. The purification process includes the following steps: ultrafiltering membrane separation of mixed water solution after chitin modification and containing high molecular weight water soluble chitin derivative inside ultrafilter so that water and water soluble small molecular weight side products can permeate via the ultrafiltering membrane while the high molecular weight water soluble chitin derivative is intercepted by the ultrafiltering membrane. The said process is simple, and has low cost, high separation effect and high product purity and yield.
Description
Technical field:
The present invention relates to a kind of method of purification of high molecular weight water soluble chitin derivative.
Background technology:
High molecular weight water soluble chitin derivative generally is to obtain through chemical modification, because it can be dissolved in neutral water, has widened the application category of chitin.At present, many producers are arranged all in the Development and Production high molecular weight water soluble chitin derivative both at home and abroad.Owing in modifying process, added a large amount of properties-correcting agent, sometimes even need under concentrated acid, alkali environment, carry out, so need after modification, separate purification.It separates purification generally is to adopt method or the size exclusion chromatography (as gel permeation chromatography) that is precipitated out in organic solvent, these method ubiquities shortcomings such as purity is low, productive rate is low, cost is high, not environmental protection, and its technological process also exists certain danger.
Summary of the invention:
Purpose of the present invention is exactly in order to overcome the above problems, and a kind of method of purification of high molecular weight water soluble chitin derivative is provided, and this method purification purity height, productive rate height and cost are low.
For achieving the above object, the present invention proposes a kind of method of purification of high molecular weight water soluble chitin derivative, the chemical modification that this method is adapted to pass through chitin prepares the process of high molecular weight water soluble chitin derivative, it is characterized in that comprising the steps: that the mixed aqueous solution with high molecular weight water soluble chitin derivative sends into ultra-fine filter, its ultra-filtration membrane surface in ultra-fine filter is separated, the impurity of water and other small molecular weight sees through ultra-filtration membrane, and high molecular weight water soluble chitin derivative is then held back by ultra-filtration membrane.
Owing to adopted above scheme, the mixed aqueous solution of high molecular weight water soluble chitin derivative only needs to filter through ultra-fine filter, and separate on the ultra-filtration membrane surface in ultra-fine filter, process is simple, and cost is low, the good separating effect of ultra-filtration membrane, purification purity height, productive rate height can be up to 99.9%.
Description of drawings:
Fig. 1 is the present invention's (method of purification of high molecular weight water soluble chitin derivative) principle schematic.
Embodiment:
Also the present invention is described in further detail in conjunction with the accompanying drawings below by specific embodiment.
As shown in Figure 1, the mixed aqueous solution (2) of high molecular weight water soluble chitin derivative is filtered through ultra-fine filter, separate on its (2) ultra-filtration membrane (1) surface in ultra-fine filter, water and other water miscible small molecule by-product see through ultra-filtration membrane (1), and high molecular weight water soluble chitin derivative is then held back by ultra-filtration membrane (1).The molecular weight cutoff value of ultra-filtration membrane (1) can be regulated.The mixed aqueous solution of high molecular weight water soluble chitin derivative (2) is to be obtained after chemical modification (synthesizing) reaction by chitin/element, and it contains a large amount of inorganic salt, alkali and water-soluble modified dose.Ultra-filtration membrane (1) is a kind of porous-film of asymmetric microvoid structure, and its aperture is 5 to 10
4Nm, it can separate colloidal particle and solute from water.Ultra-filtration membrane (1) generally in biotechnology in order to filtering microorganism, or from water, separate colloidal particle and solute.The present invention has found a kind of new purposes of ultra-filtration membrane (1), is used to the high molecular weight water soluble chitin derivative of purifying.
In order to improve purification purity, in through the concentrated solution (3) of sepn process for the first time, add deionized water, continue to repeat ultra-filtration process, through repeatedly repeating said process, soluble small molecular impurity in the high molecular weight water soluble chitin derivative material is removed, obtain highly purified product, its purity can be calculated and be controlled by concentration ratio and ultrafiltration number of times.This method also can concentrate high molecular weight water soluble chitin derivative solution simultaneously, and recyclable soluble small molecular by product.
The mixed aqueous solution of high molecular weight water soluble chitin derivative (2) needs elder generation after 200 mesh filter screens filter, and inserts in the ultra-fine filter and filters.
Embodiment: will be through the high-molecular weight water-soluble carboxymethyl chitin mixed aqueous solution (soluble small molecular by products such as this solution saliferous, alkali, chloracetate, amino acid) after modified-reaction and the neutralization, through 200 mesh filter screens (is plastic filter screen, as: the polypropylene fibre material) after the filtration, insert in the material trough of ultra-fine filter, the starter motor switch, ultra-fine filter is started working.The molecular weight cutoff value of this ultra-filtration membrane (1) is designed to 10,000.At this moment, water and soluble small molecular impurity are by being discharged by a pipeline behind the ultra-fine filter, and high-molecular weight water-soluble carboxymethyl chitin then with remaining small molecules (moisture), is got back to again by another pipeline and carried out the repetition ultrafiltration in the material trough.Behind the process ultrafiltration and concentration first time (concentration ratio is 1/6～1/5), adding deionized water to groove expires.Proceed ultra-filtration and separation, repeat 5～6 times, can obtain spissated, purity up to 99.9% the high-molecular weight carboxymethyl chitin aqueous solution, but its widespread use is as bio-medical engineering material, as the carrier of production thin, water soluble membranous type medical dressing and various organized enzymes.
For dissimilar water-solubility chitin derivatives, can suitably adjust selected ultra-filtration membrane (1) with and the molecular weight cutoff value, the input and output material pipeline pressure and the temperature of charge of initial mixing pH value of aqueous solution, equipment also should be controlled, otherwise, with having influence on the work-ing life of film, finally influence quality product.The pH value of mixed aqueous solution is preferably in about 7, and pressure range is generally at 1-5 normal atmosphere, and optimum value is about 2 normal atmosphere, and temperature should be controlled at 5-80 degree centigrade, room temperature the best.
Claims (5)
1. the method for purification of a high molecular weight water soluble chitin derivative, the chemical modification that this method is adapted to pass through chitin prepares the process of high molecular weight water soluble chitin derivative, it is characterized in that comprising the steps: the mixed aqueous solution (2) of the water-solubility chitin derivative of the pbz polymer amount behind the chitin modified-reaction is sent into ultra-fine filter, its (2) ultra-filtration membrane (1) surface in ultra-fine filter is separated, water and other soluble small molecular by product see through ultra-filtration membrane (1), and high molecular weight water soluble chitin derivative is then held back by ultra-filtration membrane (1).
2. the method for purification of a kind of high molecular weight water soluble chitin derivative as claimed in claim 1, it is characterized in that also comprising the steps: in through the concentrated solution (3) after separating, to add deionized water, continue to repeat ultra-filtration process, through repeatedly repeating said process, obtain the high molecular weight water soluble chitin derivative of required purity.
3. the method for purification of a kind of high molecular weight water soluble chitin derivative as claimed in claim 1 or 2, it is characterized in that: the mixed aqueous solution (2) of the water-solubility chitin derivative of the pbz polymer amount behind the chitin modified-reaction after 200 mesh filter screens filter, is inserted in the ultra-fine filter earlier again.
4. the method for purification of a kind of high molecular weight water soluble chitin derivative as claimed in claim 1 or 2, it is characterized in that: described high molecular weight water soluble chitin derivative mixed aqueous solution is a high-molecular weight water-soluble carboxymethyl chitin mixed aqueous solution, the molecular weight cutoff value of ultra-filtration membrane (1) is set to 1,000 to 100,000.
5. the method for purification of a kind of high molecular weight water soluble chitin derivative as claimed in claim 1 or 2, it is characterized in that: the initial mixing pH value of aqueous solution is about 7, and input and output material pipeline pressure is 1 to 5 normal atmosphere, and temperature of charge is 5-80 degree centigrade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021519668A CN1500812A (en) | 2002-11-12 | 2002-11-12 | Purifying method of high molecular weight water soluble chitin derivant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021519668A CN1500812A (en) | 2002-11-12 | 2002-11-12 | Purifying method of high molecular weight water soluble chitin derivant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1500812A true CN1500812A (en) | 2004-06-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA021519668A Pending CN1500812A (en) | 2002-11-12 | 2002-11-12 | Purifying method of high molecular weight water soluble chitin derivant |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276757A (en) * | 2011-07-29 | 2011-12-14 | 武汉大学 | Method for preparing chitin quaternary ammonium salt |
-
2002
- 2002-11-12 CN CNA021519668A patent/CN1500812A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276757A (en) * | 2011-07-29 | 2011-12-14 | 武汉大学 | Method for preparing chitin quaternary ammonium salt |
| CN102276757B (en) * | 2011-07-29 | 2012-08-08 | 武汉大学 | Method for preparing chitin quaternary ammonium salt |
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