CN1498889A - Salt of gambogicacid class compound, its preparation method, and medication combination with salt as active ingredient - Google Patents
Salt of gambogicacid class compound, its preparation method, and medication combination with salt as active ingredient Download PDFInfo
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Abstract
The salt of garcinolic acid kind of compounds is disclosed, which isa stable compound with a single structure and high feasibility and safety in medicinal research. Said salt, especially the neogarcinolate, has higher solubility in water and high anticancer effect. Its preparing process, the antineoplastic medicines using said salt as its active component, and the application of said antineoplastic medicines in treating liver cancer, intestinal cancer, lung cancer, gastric cancer, etc are also disclosed.
Description
Technical field
The present invention relates to the salt of novel gambogic acid compounds, its preparation method and be the pharmaceutical composition of active ingredient, and their application in the preparation antitumor drug with this salt.
Background technology
There are some researches show that gambogic acid compounds has restraining effect for tumour, but the solubleness of gambogic acid compounds in water is minimum, has therefore influenced their pharmaceutical use.
Educational circles was devoted to improve the research of the water solubility of gambogic acid compounds in recent years.Since gambogic acid compounds under alkaline condition particularly pH value greater than 12 condition under the instability, therefore, the research direction of educational circles concentrates on the suitable organic bases of searching in the more weak organic bases of alkalescence, with compound water-soluble to improve it with gambogic acid compounds.
People such as the outstanding Qi Dong of China Medicine University, mixture (the Chinese invention patent application in publication number CN1309125A, open day August 22 calendar year 2001) with arginine and Methionin and gambogic acid compounds has been proposed, think the water solubility that can improve gambogic acid compounds, but fail to announce experimental data and solubleness.
Summary of the invention
Technical problem to be solved by this invention provides that a class is new, that water solubility is high, the salt of gambogic acid compounds with pharmaceutical use.
Another technical problem to be solved by this invention provides a kind of method for preparing above-mentioned salt.
The further technical problem to be solved of the present invention provides a kind of antitumor medicine composition.
Another technical problem to be solved by this invention provides the application of above-mentioned salt in preparation medicine for treating tumor thing.
The general formula of the salt of gambogic acid compounds provided by the invention is:
R=H or R=OH in the formula, X represents alkalimetal ion.
The gambogic acid compounds of indication of the present invention is morellic acid or neogambogic acid, and its preparation method can be with reference to " Jiangxi Medical College's journal " 1980, (2): 1.With Gamboge 100g, pulverize and add acetone 500ml, reflux half an hour, triplicate, united extraction liquid is concentrated into driedly, obtains to contain the mixture total cambogic acid that R is H and OH.
Total cambogic acid 10g adds acetone solution, and lysate extracts the Rf value and be 0.49 color spot after chromatographic separation, use the acetone wash-out, is concentrated into driedly, and the compound that obtains R=H is a morellic acid.
Total cambogic acid 10g adds acetone solution, and lysate extracts the Rf value and be 0.23 color spot after chromatographic separation, use the acetone wash-out, is concentrated into driedly, and the compound that obtains R=OH is a neogambogic acid.
The present invention has broken through research range in the past, with the inorganic salt of gambogic acid compounds as research object, the mixture of it and gambogic acid compounds has basic difference, one of its difference is that it is the compound of stable single structure, and mixture is the unsettled material that two compounds are combined by Intermolecular Forces.Therefore, salt compounds provided by the invention more helps the study of pharmacy of medicine and the research of medicine toxic side effect, avoids other unknown untoward reaction of bringing because of non-main medicinal ingredients thereby help it in study of pharmacy, brings injury to the patient.Therefore, salt compounds provided by the invention is than mixture more feasible and security on drug research.
The contriver finds that through a large amount of experiments the mixture that the water solubility of salt provided by the invention has more in the past is significantly improved, and specifically sees Table:
| Title | Water solubility (g/ml) |
| Total cambogic acid | 1∶500 |
| Neogambogic acid | 1∶500 |
| Morellic acid | 1∶500 |
| Total cambogic acid-Methionin mixture | 1∶70 |
| Morellic acid-arginine mixture | 1∶70 |
| Neogambogic acid-Methionin mixture | 1∶70 |
| Total cambogic acid sodium | 1∶20 |
| Morellic acid sodium | 1∶20 |
| Neogambogic acid sodium | 1∶20 |
| Morellic acid potassium | 1∶20 |
| Neogambogic acid potassium | 1∶20 |
The present invention finds that simultaneously the anticancer effect mixture more in the past of salt provided by the invention is significantly improved, and the most outstanding with shindox zanthogenate especially.Find also that by experiment salt provided by the invention to normal hemopoietic system and not obviously influence of white corpuscle, does not have obvious blood vessel irritation in the kill tumor cell.Therefore salt provided by the invention has higher pharmaceutical use.
The preferred version of the salt of above-mentioned gambogic acid compounds is: X is sodium ion or potassium ion.Sodium ion or potassium ion are easier to combine salify with gambogic acid compounds, and the stability of salt is stronger.
The preparation method of the salt of gambogic acid compounds provided by the invention: gambogic acid compounds and alkali-metal mineral alkali are with 1: the ratio of 0.8-2.0 (equivalence ratio), under the condition that the pH8-12 buffer reagent exists, in water and/or 95% following alcoholic solvent, react.The preferred version of gambogic acid compounds and alkali-metal mineral alkali equivalence ratio is a gambogic acid compounds: alkali-metal mineral alkali=1: 0.8-1.5.Alkali-metal mineral alkali is preferably sodium hydroxide, potassium hydroxide, yellow soda ash, salt of wormwood, sodium bicarbonate or saleratus.The preferred version of buffer reagent is carbonate-bicarbonate buffer agent of pH9-11.Certainly buffer reagent also can be boric acid-borax buffer reagent, glycine-sodium hydroxide buffer reagent, Ches-sodium hydroxide buffer reagent, Taps-sodium hydroxide buffer reagent, the Caps-sodium hydroxide buffer reagent of pH8-12.
Adopt method provided by the invention can make gambogic acid compounds under stable status, form stable salt with the mineral alkali reaction.
Antitumor medicine composition provided by the invention: the salt that contains the above-mentioned general formula of effective therapeutic dose is active ingredient, and contains one or more pharmaceutically acceptable carriers.It preferably contains the active ingredient of weight ratio 0.1-99.9%, most preferably contains the active ingredient of weight ratio 1-99.9%.Described salt preferred be R=H or is R=OH.Described salt also can be the mixture of the arbitrary proportion of the salt of the salt of R=H and R=OH.
Mixture provided by the invention and pharmaceutical composition can be used for preparation treatment lung cancer, liver cancer, intestinal cancer, cancer of the stomach, breast cancer, random nest cancer, leukemia medicament.
Pharmaceutically acceptable carrier mentioned above is meant thinner, vehicle, weighting agent, tackiness agent, wetting agent, disintegrating agent, absorption enhancer, absorption carrier, lubricant, flavouring agent, sweeting agent etc.
Its formulation of pharmaceutical composition of the present invention comprises: tablet, capsule, granule, oral liquid, injection etc.Its preparation method can be according to the conventional production method preparation of pharmaceutical field.Active ingredient is mixed with one or more carriers, be made into required formulation then.
The amount of application of the mixture of general formula of the present invention can be according to variations such as the type of route of administration, patient's age, body weight, the disease for the treatment of and severity, and its per daily dose can be 0.01-100mg/kg, preferred 0.1-100mg/kg, but one or many is used.
Embodiment
The following examples can make the professional more fully understand the present invention, but limit this patent never in any form.
The preparation of example 1. morellic acid sodium
Get morellic acid 10g in beaker, add an amount of distilled water, stir, below 10 ℃, yellow soda ash-sodium bicarbonate buffer liquor to the morellic acid that slowly adds pH9-11 dissolves fully, after stirring, filter, the filtrate lyophilize, 11g morellic acid sodium salt.
The preparation of example 2. neogambogic acid sodium
Get neogambogic acid 10g in beaker, add an amount of distilled water, stir, below 10 ℃, yellow soda ash-sodium bicarbonate buffer the liquid that adds a small amount of pH9-11 slowly adds 1 NNaOH solution to neogambogic acid again and dissolves fully, filters, the filtrate lyophilize gets 11g neogambogic acid sodium salt.
The preparation of example 3. morellic acid potassium
Get morellic acid 10g in beaker, add 50% an amount of ethanol, stir, below 10 ℃, slowly add salt of wormwood-saleratus buffer reagent of people pH9-11, slowly add 1 NKOH solution again, to morellic acid dissolving fully, filter, the filtrate lyophilize gets 11g morellic acid sylvite.
The preparation of example 4. morellic acid sodium injections
Morellic acid sodium 10g
Dextran 40 g
Morellic acid sodium 10g is added an amount of water for injection dissolving, and Dextran 40 g adds an amount of water for injection dissolving, and two solution are mixed, add injection and be diluted with water to 2000ml, with the filtering with microporous membrane of 0.22um, under the aseptic condition, be loaded on respectively in the 10ml cillin bottle, sabot, send in the freeze drying box, after the lyophilize, outlet, Zha Gai gets final product.
The preparation of example 5. morellic acid potassium sheets
Morellic acid potassium 10g
Starch 80g
Starch slurry (8%) is an amount of
Magnesium Stearate 0.4g
Morellic acid potassium is added the starch uniform mixing, add 8% starch slurry and make softwood, granulate with 14 order nylon mesh, 70-80 ℃ of drying adds Magnesium Stearate, and through the whole grain of 10-12 order iron wire sieve, mixing is with 12mm punch die compressing tablet.
The preparation of example 6. morellic acid sodium granules
Morellic acid sodium 10g
Soluble starch 80g
Icing Sugar 20g
Essence is an amount of
Morellic acid sodium is water-soluble, add starch 80g, Icing Sugar 20g, it is an amount of to add essence again, and mixing is granulated with the 14-16 mesh sieve, and is dry below 60 ℃, packing.
The preparation of example 7. morellic acid natrium capsules
Morellic acid sodium 10g
Microcrystalline Cellulose 40g
Lactose 60g
Sodium Hydroxymethyl Stalcs 4g
Starch slurry is an amount of
Magnesium Stearate 1g
Micropowder silica gel 1g
Morellic acid sodium, Microcrystalline Cellulose, lactose, Sodium Hydroxymethyl Stalcs sieves respectively, and mix, add starch slurry and make softwood in right amount, to cross 20 mesh sieves and granulate, wet granular is dry down in 50 ℃, dried particle is crossed the whole grain of 20 mesh sieves, with Magnesium Stearate, micropowder silica gel mixing, can capsule.
The preparation of example 8. neogambogic acid sodium oral liquids
Neogambogic acid sodium 10g
Asccharin 0.5g
Essence 0.1g
Water for injection 1000ml
After neogambogic acid sodium, asccharin, essence is dissolved in water for injection respectively, mixed, be diluted to 1000ml, packing, promptly.
Embodiment 9, inhibition knurl strain test:
Suppress the test of knurl strain selectivity with morellic acid sodium:
1. take by weighing above-claimed cpd 50mg, be dissolved in water, after the dissolving, be made into the stock solution of 2.5mg/ml fully.
2. it is an amount of to get above-mentioned stock solution, adds the cell complete culture solution, and mixing is mixed with drug level successively and is respectively: 16ug/ml, 8ug/ml, 4ug/ml, 2ug/ml, 1ug/ml, 0.5ug/ml, the nutrient solution of 0.25ug/ml.
3. cultivate by standard, use microplate reader, 570nm photometry density OD.
4. cell survival rate=(dosing cell OD/ control cells OD) * 100
Cell inhibitory rate (I)=100-cell survival rate
With the drug level C of correspondence, cell inhibitory rate I, by formula 1g (I/100-)=A+B1gC carries out line and returns, behind the equation, IC50=1g
-1[(A)/B].
Following table is an experimental result:
| Clone | IC50 |
| Lung cancer D6 cell | 5.12 |
| Liver cancer SMMC7721 cell | 2.21 |
| Intestinal cancer SW480 cell | 2.15 |
| Cancer of the stomach MNK28 cell | 4.28 |
| Breast cancer Bcap37 cell | 3.69 |
| Ovarian cancer HO-8910PM cell | 4.74 |
| The leukemia K 562 cell | 4.92 |
From last table we as can be seen morellic acid sodium the tumour cell of liver cancer and intestinal cancer is had very strong restraining effect, lung cancer, cancer of the stomach, breast cancer, ovarian cancer, leukemic tumour cell are had suitable restraining effect.Equally, the above-mentioned tumour cell of salt pair of other gambogic acid compounds provided by the invention also has identical restraining effect
The simultaneous test of the influence of 10 couples of liver cancer SMMC7721 of embodiment cell growth
In the present embodiment the material in the following table has been carried out simultaneous test to the influence of liver cancer SMMC7721 cell growth.
Experimental technique is with embodiment 9
Influence to the growth of liver cancer SMMC7721 cell
| The compound title | IC50(ug/ml) |
| Total cambogic acid | 4.67 |
| Morellic acid | 4.65 |
| Neogambogic acid | 3.12 |
| Morellic acid arginine mixture | 4.15 |
| Neogambogic acid Methionin mixture | 3.26 |
| Morellic acid sodium | 2.21 |
| Neogambogic acid sodium | 1.96 |
| Morellic acid potassium | 2.45 |
| New rattan acid potassium | 2.01 |
From above-mentioned data declaration: the mixture of the sodium of gambogic acid compounds, potassium compounds salt and other gambogic acid compounds itself and other organic bases is compared, liver cancer SMMC7721 is had the high advantage of obvious antitumor activity, and especially the sodium of neogambogic acid, potassium compounds salt antitumor activity are the strongest.
The simultaneous test of the influence of 11 couples of intestinal cancer SW480 of embodiment cell growth
In the present embodiment the material in the following table has been carried out simultaneous test to the influence of intestinal cancer SW480 cell growth.
Experimental technique is with embodiment 9
Influence to the growth of intestinal cancer SW480 cell
| The compound title | IC50(ug/ml) |
| Total cambogic acid | 2.95 |
| Morellic acid | 3.11 |
| Neogambogic acid | 2.01 |
| Morellic acid arginine mixture | 3.25 |
| Neogambogic acid Methionin mixture | 2.84 |
| Morellic acid sodium | 2.15 |
| Morellic acid potassium | 2.07 |
| Neogambogic acid sodium | 1.37 |
| Neogambogic acid potassium | 1.26 |
From above-mentioned data declaration: the mixture of the sodium of gambogic acid compounds, potassium compounds salt and other gambogic acid compounds itself and other organic bases is compared, intestinal cancer SW480 is had the high advantage of obvious antitumor activity, and especially the sodium of neogambogic acid, potassium compounds salt antitumor activity are the strongest.
The restraining effect that example 12. neogambogic acid sodium are transplanted the Heps solid tumor to mouse transplanted sarcoma S180, mouse
Medicine preparation: quantitatively take by weighing neogambogic acid sodium, add 0.9%NaCl solution and be made into desired concn.
Route of administration: ip
The administration cycle: the 24h successive administration is 9 days after inoculation, and once a day, execution in first day is dissected after the drug withdrawal.
Dosage is provided with: establish five groups altogether
The A group: neogambogic acid sodium group (8,4,2mg/kg)
B group: neogambogic acid Methionin (8mg/kg)
C group: blank group (physiological saline 0.4ml/20g)
Mouse type: Kunming mouse body weight 18-22g
Administration volume: 0.4ml/20g
Experimental technique: get 50 of above-mentioned specification mouse, press the inoculation of transplanted tumor organon, inoculate back 24 hours and claim mouse heavy, and be divided into 5 groups at random, every group 10, male and female half and half, physiological saline and neogambogic acid Methionin are respectively the yin, yang control group, and neogambogic acid sodium group is established high, medium and low three dosage groups.After drug withdrawal, weighed in first day, put to death and separation knurl piece, claim that knurl is heavy and carry out statistical procedures (t check)
Table 1 neogambogic acid sodium ip transplants the restraining effect (x ± SD) of S180 to mouse
| Group | Mouse number (only) | Dosage mg/kg | Body weight (g) | Knurl heavy (g) | Tumour inhibiting rate (%) | |
| Before the administration | After the administration | |||||
| C group A group B group | ?10 ?10 ?10 ?10 ?10 | ?8 ?4 ?2 ?8 | ?19.4±1.21 ?19.4±1.18 ?19.5±1.24 ?19.4±1.26 ?19.5±1.20 | ?25.9±1.96 ?23.9±1.68 ?25.6±1.76 ?26.3±1.82 ?23.4±1.58 | ?2.46±0.48 ?0.46±0.20 **?1.32±0.32 **?2.13±0.44 **?0.76±0.29 ** | ?81.3 ?46.3 ?13.4 ?69.1 |
*P＜0.01 is compared with the physiological saline control group
Table 2 neogambogic acid sodium ip transplants the restraining effect (x ± SD) of Heps solid tumor to mouse
| Group | Mouse number (only) | Dosage mg/kg | Body weight (g) | Knurl heavy (g) | Tumour inhibiting rate (%) | |
| Before the administration | After the administration | |||||
| C group A group B group | ?10 ?10 ?10 ?10 ?10 | ?8 ?4 ?2 ?8 | ?20.1±1.42 ?20.0±1.41 ?20.3±1.44 ?19.9±1.38 ?19.9±1.40 | ?27.6±2.14 ?25.8±2.13 ?26.9±2.03 ?27.4±2.26 ?24.9±2.10 | ?2.52±0.42 ?0.59±0.28 **?1.17±0.36 **?1.73±0.40 **?0.77±0.29 ** | ?76.6 ?53.6 ?31.3 ?69.4 |
*P＜0.01 is compared with the physiological saline control group
Experimental result: the result shows with the physiological saline control group and compares, neogambogic acid sodium has the growth effect that suppresses mouse S180, transplantability Heps solid tumor very significantly, and weight of mice do not had obvious influence, and the tumor-inhibiting action of neogambogic acid sodium is better than neogambogic acid Methionin.
Example 13. morellic acid sodium are to the prolongation effect of mice transplanted tumor life
Medicine preparation: quantitatively take by weighing morellic acid sodium, add 0.9%NaCl solution and be made into desired concn.
Route of administration: ip
The administration cycle: in the 24h administration of inoculation back, once a day, administration is 10 days altogether, observes altogether after the administration 60 days.
Dosage is provided with: establish five groups altogether
A group: morellic acid sodium group (1.50,0.75,0.50)
B group: morellic acid Methionin (1.5)
C group: blank group (physiological saline 0.4ml/20g)
Mouse type: Kunming mouse body weight 18-22g
Administration volume: 0.4ml/20g
Experimental technique: get 50 of above-mentioned specification mouse, press the inoculation of transplanted tumor organon, after the inoculation, be divided into 5 groups at random, 10 every group, male and female half and half, physiological saline and morellic acid Methionin are respectively the yin, yang control group, and morellic acid sodium group is established high, medium and low three dosage groups.After the administration, observe tumor-bearing mice survival fate (survival more than 60 days mouse by 60 days) and survival rate (by survival more than 60 days and asymptomatic mouse), and carry out statistical procedures (t check)
Table 1 morellic acid sodium ip prolongs vital action to EAC ascitic type mice-transplanted tumor
| Group | Mouse number (only) | Dosage mg/kg | The survival fate (x ± SD) | Increase in life span (%) | Survival rate (%) |
| ?C ?A ?B | ?10 ?10 ?10 ?10 ?10 | ?1.50 ?0.75 ?0.50 ?1.50 | ?10.6±2.41 ?36.3±15.31 **?24.1±5.83 **?18.3±3.81 **?31.7±7.46 ** | ?242.6 ?127.4 ?72.6 ?199.1 | ?30 ?0 ?0 ?0 |
*P＜0.01 is compared with the physiological saline control group
Table 2 morellic acid sodium ip prolongs vital action to Heps ascitic type mouse transplanted sarcoma
| Group | Mouse number (only) | Dosage mg/kg | The survival fate (x ± SD) | Increase in life span (%) | Survival rate (%) |
| ?C ?A ?B | ?10 ?10 ?10 ?10 ?10 | ?1.50 ?0.75 ?0.50 ?1.50 | ?10.5±2.16 ?34.5±14.63 **?23.0±8.71 **?17.3±5.64 **?30.1±12.45 ** | ?228.6 ?119.1 ?64.8 ?186.7 | ?20 ?0 ?0 ?10 |
*P＜0.01 is compared with the physiological saline control group
Experimental result: the result shows, compare with the physiological saline control group, morellic acid sodium has the EAC of prolongation ascitic type mouse, Heps ascitic type mouse survival fate effect (P＜0.01), and increase in life span and the survival rate of EAC ascitic type mouse, Heps ascitic type mouse all is better than morellic acid Methionin.
Example 14. neogambogic acid sodium are to the influence of leukocyte count in the mouse blood
Experimental technique: get the 18-22g mouse, press the transplanted tumor organon, inoculation S180, inoculation is afterwards weighed and be divided into 5 groups at random next day, every group 10, male and female half and half, ip administration, 9d altogether, weigh and get blood by the eye socket vein in after drug withdrawal first day, the microscopy total white blood cells, anatomical isolation knurl piece is simultaneously weighed and is carried out statistical procedures.
The A group: neogambogic acid sodium group (8,4,2mg/kg)
B group: neogambogic acid Methionin (8mg/kg)
C group: blank group (physiological saline 0.4ml/20g)
| Group | Mouse number (only) | Dosage mg/kg | Body weight (g) | ?WBC ?No./mm 3 | Knurl heavy (g) | Tumour inhibiting rate (%) | |
| Before the administration | After the administration | ||||||
| C group A group B group | ?10 ?10 ?10 ?10 ?10 | ?8 ?4 ?2 ?8 | ?19.1±1.24 ?19.3±1.28 ?19.1±1.14 ?19.3±1.30 ?19.2±1.20 | ?25.7±1.61 ?24.0±1.23 ?25.2±1.32 ?26.0±1.34 ?23.4±1.58 | ?11233±3617 ?10714±3612 ?9842±3578 ?11715±3964 ?97165±3741 | ?2.38±0.26 ?0.40±0.18 **?0.95±0.20 **?1.62±0.34 **?0.57±0.19 ** | ?83.2 ?60.1 ?31.9 ?76.0 |
*P＜0.01 is compared with the physiological saline control group
Experimental result: the result shows with the physiological saline control group and compares that neogambogic acid sodium has the growth effect that suppresses mouse S180 very significantly, and its effect is better than neogambogic acid Methionin, and white corpuscle in the mouse peripheral blood is not had obvious influence.
Example 15. neogambogic acid natremia pipe irritation tests
Experimental technique: get 2 of body weight 2.2-2.4kg healthy rabbits, rabbit is placed fixer, rabbit left side auricular vein instillation neogambogic acid sodium injection 8mg/kg amounts to 6ml, auris dextra instillation chloride injection is dared liquid 6ml/kg, drip velocity is 20 droplets/minute, every morning instils once, a continuous week.Observe left and right sides auricular vein reaction, observe the injection site blood vessel every day and have or not rubescent, oedema, have or not oozing of blood on every side, touch blood vessel and have or not the hardening phenomenon, left and right sides ear is more now examined.After time administration 12 hours,, take off left and right sides ear, cut sections for microscopic examination with sacrifice of animal.
Experimental result: after two rabbit administrations, every day, left and right ear was all normal, and is not rubescent, no oedema, and histopathological examination is Non Apparent Abnormality also.The blood vessel irritation test of neogambogic acid sodium shows that this medicine does not have obvious local irritant effect to the rabbit auricular vein.
Claims (12)
1. the salt that has the gambogic acid compounds of following general formula
R=H or R=OH in the formula, X represents alkalimetal ion.
2. the salt of gambogic acid compounds as claimed in claim 1 is characterized in that X is a sodium ion.
3. the salt of gambogic acid compounds as claimed in claim 1 is characterized in that X is a potassium ion.
4. the preparation method of the salt of the gambogic acid compounds of claim 1, it is characterized in that gambogic acid compounds and alkali-metal mineral alkali are with 1: the ratio of 0.8-2.0 (equivalence ratio), under the condition that the pH8-12 buffer reagent exists, in water and/or 95% following alcoholic solvent, react.
5. the preparation method of the salt of gambogic acid compounds as claimed in claim 4, the preferred version that it is characterized in that gambogic acid compounds and alkali-metal mineral alkali equivalence ratio is a gambogic acid compounds: alkali-metal mineral alkali=1: 0.8-1.5.
6. the preparation method of the salt of gambogic acid compounds as claimed in claim 4 is characterized in that alkali-metal mineral alkali is preferably sodium hydroxide, potassium hydroxide, yellow soda ash, salt of wormwood, sodium bicarbonate or saleratus.
7. the preparation method of the salt of gambogic acid compounds as claimed in claim 4, the preferred version that it is characterized in that buffer reagent is carbonate-bicarbonate buffer agent of pH9-11.
8. the pharmaceutical composition that is used for the treatment of tumour is characterized in that wherein containing the salt and the pharmaceutically acceptable carrier of the claim 1 of effective therapeutic dose.
9. the pharmaceutical composition that is used for the treatment of tumour as claimed in claim 8, that it is characterized in that described salt preferably is R=H.
10. the pharmaceutical composition that is used for the treatment of tumour as claimed in claim 8, that it is characterized in that described salt preferably is R=OH.
11. the pharmaceutical composition that is used for the treatment of tumour as claimed in claim 8 is characterized in that described salt is the mixture of arbitrary proportion of the salt of the salt of R=H and R=OH.
12. any salt among the claim 1-3, the application in preparation treatment lung cancer, liver cancer, intestinal cancer, cancer of the stomach, breast cancer, ovarian cancer, leukemia medicament.
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| CNB021483213A CN1315837C (en) | 2002-11-04 | 2002-11-04 | Salt of gambogicacid class compound, its preparation method, and medication combination with salt as active ingredient |
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