CN1486702A - Injection for eliminating wrinkles and scar and its prepn - Google Patents
Injection for eliminating wrinkles and scar and its prepn Download PDFInfo
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- CN1486702A CN1486702A CNA03155833XA CN03155833A CN1486702A CN 1486702 A CN1486702 A CN 1486702A CN A03155833X A CNA03155833X A CN A03155833XA CN 03155833 A CN03155833 A CN 03155833A CN 1486702 A CN1486702 A CN 1486702A
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Abstract
The present invention belongs to the field of tissue engineering. The injection consists of 10-40 million hypodermal fibroblasts and 2-4 ml of 5% concentration glucose injection. The preparation process includes extracorporela amplification culture of patient's self hypodermal fibroblasts, and adding glucose injection to the hypodermal fibroblasts to prepare the suspension. The present invention has the advantages of baking biological material from the patient himself, no rejection, no side effect, obvious beautifying effect, natural expression and lasting effect for several years.
Description
Technical field
The present invention relates to injection of a kind of Wrinkle-and scar-removing and preparation method thereof, belong to field of tissue engineering technology.
Background technology
People adopt the collagen protein of injection cattle to treat wrinkle and cicatrix before 20 years, but it anaphylaxis can occur, can be organized simultaneously faster to absorb the time that can only keep some months.In order to overcome this anaphylactoid shortcoming, some hospital adopts and becomes collagen protein to inject from the body leather, and the result is that preparation process needs a large amount of skin, and still can be organized very fast absorption after the injection.Many in recent years people adopt creotoxin to inject and treat wrinkle and cicatrix, and its result also can only keep the relatively shorter time, and the expression utmost point of treatment back therapist is unnatural.
Summary of the invention
In order to overcome the weak point of these products, the purpose of this invention is to provide a kind of efficient, permanent, injection of the Wrinkle-and scar-removing of back nature more.
The autologous skin that this injection has adopted the people is isolated dermal fibroblast through digestion, carries out amplification in vitro, is then injected into the wrinkle or the cicatrix position of therapist, and these cells produce collagen tissue in its injection site, smoothes away wrinkles and fills and leads up cicatrix.
Another object of the present invention provides the preparation method of this injection.
For achieving the above object, the present invention is by the following technical solutions:
A kind of injection of Wrinkle-and scar-removing is made up of the glucose injection of 1,000 ten thousand-4,000 ten thousand dermal fibroblasts and 2-4ml concentration 5%.
Described dermal fibroblast is to obtain through amplification in vitro, cultivation deriving from the dermal fibroblast of patient from body.
A kind of preparation method of injection of Wrinkle-and scar-removing, be to derive from the patient to cultivate at amplification in vitro from the dermal fibroblast of body, add the glucose injection of 2-4ml concentration 5% again according to per 1,000 ten thousand-4,000 ten thousand dermal fibroblasts, make suspension.
The present invention a kind ofly is expelled to the Wrinkle-and scar-removing product that wrinkle, cicatrix position are repaired with patient's autologous skin cell external once more through propagation, after cultivating, collagen protein makes rill and cicatrix is long puts down thereby this product produces at these positions, smoothing away wrinkles and cicatrix can improve simultaneously the environment of local skin again, playing the effect of young U.S. face.
Advantage of the present invention: the biomaterial that the present invention uses is taken from the patient from body, and no rejection has no side effect, therapist with after improve looks evident in efficacyly, look natural, and the effect effect is lasting, can keep the several years.
The invention will be further described for the following specific embodiment.
The specific embodiment
The preparation of the injection of embodiment 1, Wrinkle-and scar-removing
One. from the extraction of body healthy cell
With behind 70% the alcohol disinfecting ear or arm inboard, use then 10% lignocaine and 100,000/ epinephrine carry out local surfaces anesthesia.Take off with the epidermis and the dermal tissue of cutisector with 4 square millimeters, put into tissue and preserve in the liquid (DMEM and 10% hyclone, U.S. Hyclone company), wound site is made a pin sew up, adhesive bandage covers.
Two. the preparation of cell suspension
1. tissue digestion (Sanya CO2 incubator): the skin histology piece is positioned over to add 2ml concentration in the 35mm culture dish be pancreatin/EDTA liquid of 0.05%, 37 ℃, 10 minutes; The DMEM+10%FBS culture fluid stops.
2. cell separation: postdigestive piece of tissue is shredded and breaks up with suction pipe.
3. centrifugal: the cell harvesting after dispelling to centrifuge tube, Backman company temperature control centrifuge, 25 ℃, 1000 commentaries on classics/min, centrifugal 5 minutes, abandoning supernatant.
4. primitive cell culture: add the low serum true chrotoplast culture fluid (Sigma company, the U.S.) of 1ml and blow outstandingly in centrifuge tube, 1 * 35mm culture dish is gone in inoculation; Sanya CO
2Incubator, 37 ℃, 5% CO
2, cultivate.
5. passage cell is cultivated: changed liquid 1 time every 2-3 days, go down to posterity after 50-70% compiles cell when cell reaches.
6. preparation injection: the dermal fibroblast after subculture in vitro separately to the 4-6 week, adding 2-4ml concentration by per 1,000 ten thousand-4,000 ten thousand fibroblasts is 5% glucose injection, is mixed with suspension.
Three. product detects
1. virus detects: after around skin histology is cultivated, get the check that culture fluid that 1ml contains cell send Beijing infectious hospital to carry out HIV and hepatitis virus, determine that this is virus-free cell.
2. immunoreation test: around the 0.1ml cell suspending liquid is carried out skin test (with penicillin skin test method) to the operation receiveing person, to guarantee not having immunoreation.
3. finished product detection
1) outward appearance: this product is the pale suspension.
2) sterility test: undertaken by " Chinese biological goods rules " (2000 editions) general rule " biological product sterility test rules " A/B item, the result meets aseptic requirement.
The storage of product and transportation should be carried out in 2 ℃-10 ℃, shady and cool, dry, the environment that cleans, avoid sunlight direct projection, non-corrosiveness gas, no weight.
Embodiment 2, clinical report
One. case standard (case load 28 examples)
1. include standard in: the age (comprised 55 years old) below 55 years old; The wrinkle person be forehead, near the eyes, perioral aging lines; The cicatrix person is acne, wound cicatrix (magnitude range no requirement (NR)).
2. exclusion standard: the age is patient more than 55 years old; Autoimmune disease is arranged; The chronic skin patient is arranged; The infection patient is arranged.
Two. prepare injection: the method according to embodiment 1 is carried out.
Three. treatment
1. check before patient's art: hematuria is routine, electrocardiogram, hepatic and renal function, HIV, HbsAg just.
With the injection site with 70% alcohol disinfecting, the lignocaine of local injection 1%, perhaps epidermis is with 2.5% lignocaine and the anesthesia of 2.5% Astral512 ointment.
3. standby in the syringe with 1.5ml cell suspension suction 3ml.
4. get No. 4.5 long syringe needles of 2.2cm, adopt multiple spot inclined-plane (20 °-45 ° of the angles of syringe needle and skin) that cell suspension is injected into the upper strata and the middle level of skin corium, the 15-20 point is chosen in each injection site, injects cell suspension 0.05-0.1ml at every.Should it be turned white the tension of injection site epidermis during injection, should make the injection site leave the disperse space during injection.Wrinkle or cicatrix portion are all injected, injected the back and used ice bag external application 2 hours.
Injection site has sensations such as mild swelling, numbness in the postoperative 24 hours, does not generally have other part and general reaction.
The course of treatment: general wrinkle injection 2 times, big wrinkle or cicatrix injection three times, per injection is 2-4 week at interval.
Four, clinical follow, nurse:
1. after the operation, apply injection place 2 hours with ice bag.
2. observe the situation of part and whole body.
3. secondary oral vitamin C every day (each 200mg, every day is 400mg altogether), perhaps the part is put on the skin and is contained ascorbic facial cream, about 6 months.
4. postoperative was prevented tanning by the sun and the careful zest cosmetics of using in 3 days.
Five. efficacy analysis
Through to the analysis of cases of 28 examples, wherein the case of a shot has 20 examples, double injection 8 examples are arranged, 6 months observing times, the result has 24 examples (its satisfaction rate is 85.7%) for my approbation of patient, and its wrinkle, cicatrix obviously disappear, and the dermal matrix situation obviously improves.
The person observed 2 years so far wherein to have the individualized treatment, the complete obiteration of its wrinkle, cicatrix, skin smooth and high resilience.
Claims (3)
1, a kind of wrinkle removal is removed the injection of scar, and it is characterized in that: this injection is made up of the glucose injection of 1,000 ten thousand-4,000 ten thousand dermal fibroblasts and 2-4ml concentration 5%.
2, the injection of Wrinkle-and scar-removing according to claim 1 is characterized in that: described dermal fibroblast be origin come from the patient from the dermal fibroblast of body through amplification in vitro, cultivate and obtain.
3, a kind of wrinkle removal is removed the preparation method of the injection of scar, it is characterized in that: this method be with derive from the patient from the dermal fibroblast of body in amplification in vitro and cultivation, add the glucose injection of 2-4ml concentration 5% again according to per 1,000 ten thousand-4,000 ten thousand dermal fibroblasts, make suspension.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB03155833XA CN1206963C (en) | 2003-08-25 | 2003-08-25 | Injection for eliminating wrinkles and scar and its prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB03155833XA CN1206963C (en) | 2003-08-25 | 2003-08-25 | Injection for eliminating wrinkles and scar and its prepn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1486702A true CN1486702A (en) | 2004-04-07 |
| CN1206963C CN1206963C (en) | 2005-06-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB03155833XA Expired - Lifetime CN1206963C (en) | 2003-08-25 | 2003-08-25 | Injection for eliminating wrinkles and scar and its prepn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1206963C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415875C (en) * | 2005-11-03 | 2008-09-03 | 毛曦 | Process for preparing self-fibroblast by skin prefibroblast |
| CN100421731C (en) * | 2006-01-11 | 2008-10-01 | 黄靖西 | Injection liquid of human autogenous collagen living cell and preparation method thereof |
| CN102205146A (en) * | 2011-04-12 | 2011-10-05 | 王影 | Stem cell repairing material as well as preparation method and application thereof |
| CN102802621A (en) * | 2009-06-17 | 2012-11-28 | 阿德兰斯研究学院有限公司 | Methods and compositions for increasing trichogenic potency of dermal cells |
| CN108066820A (en) * | 2017-12-28 | 2018-05-25 | 江苏艾尔康生物医药科技有限公司 | A kind of auto derma fibroblast parenteral solution |
-
2003
- 2003-08-25 CN CNB03155833XA patent/CN1206963C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415875C (en) * | 2005-11-03 | 2008-09-03 | 毛曦 | Process for preparing self-fibroblast by skin prefibroblast |
| CN100421731C (en) * | 2006-01-11 | 2008-10-01 | 黄靖西 | Injection liquid of human autogenous collagen living cell and preparation method thereof |
| CN102802621A (en) * | 2009-06-17 | 2012-11-28 | 阿德兰斯研究学院有限公司 | Methods and compositions for increasing trichogenic potency of dermal cells |
| CN102205146A (en) * | 2011-04-12 | 2011-10-05 | 王影 | Stem cell repairing material as well as preparation method and application thereof |
| CN108066820A (en) * | 2017-12-28 | 2018-05-25 | 江苏艾尔康生物医药科技有限公司 | A kind of auto derma fibroblast parenteral solution |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1206963C (en) | 2005-06-22 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: LV WEIGUANG Free format text: FORMER OWNER: BEIJING YILING BIOENGINEERING CO., LTD. Effective date: 20060407 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20060407 Address after: 100022, No. 1707, Jianguo Road, No. 98, Jianguo Road, Beijing, Chaoyang District, A Patentee after: Lv Weiguang Address before: 101500 Beijing City Industrial Development Zone in Miyun Dashenglu No. 60 Patentee before: Beijing Yiling Bioengineering Co.,Ltd. |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20050622 |